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Undergraduate research is a "high-impact" educational practice that enriches student learning and facilitates student career advancement. This sequential explanatory mixed methods study, composed of a quantitative online questionnaire followed by qualitative focus group interviews, sought to explore undergraduate student attitudes on research and elicit perceived facilitators and barriers to undergraduate research engagement. The survey respondents (N = 377), all undergraduate health sciences students at McMaster University in Hamilton, Ontario, generally had positive attitudes toward undergraduate research, but had polarized perceptions of its accessibility, supportiveness, and appreciation. Follow-up focus group interviews with selected participants (N = 11) revealed four main themes: (1) the hidden curriculum of undergraduate research; (2) the paucity of meaningful research work for emerging student researchers; (3) the administrative barriers within the undergraduate research landscape; and (4) the inequitable access to undergraduate research opportunities. This study's findings suggest potential avenues to improve the undergraduate student research experience. more...

This review aims to analyse the published data on preclinical and human experimental and clinical adenosine modulation for pain management. We summarise the translatability of the adenosine pathway for further drug development and aim to reveal subgroups of pain patients that could benefit from targeting the pathway. more...

Despite increasing awareness, food loss and waste in food supply chains remains a significant challenge resulting in environmental risks and ethical concerns, shaped by the conflicting logics of abundance and scarcity. Systemic overproduction, driven by high-quality demands and the perishability of fresh food, underscores the need for new approaches to food procurement. This article explores the relationship between public procurement processes and significant loss of fresh fruit and vegetables in Denmark. Two major Danish wholesalers and Copenhagen municipality's food procurement agency formed the empirical basis for an ethnographic study undertaken between 2020 and 2023. The study involved participant observation at the wholesalers, semi-structured interviews, and informal conversations with a broad range of actors across the public food procurement chain. Findings revealed that the food quality requirements that guide everyday practices of quality assessment, both contractually and culturally, are being pushed to the limits of what growing seasons, shelf-life characteristics and distribution systems can accommodate. This results in practices where food produce that is still usable is thrown away. This article emphasizes the need for novel understandings of quality parameters in order to (re)design structures in the public food procurement process so that reducing food loss can be adopted as a regulatory tool within procurement contracts. [ABSTRACT FROM AUTHOR] more...

Drawing on a recent doctoral research project that examined the everyday life perspectives during kindergarten mealtime, this paper discusses the methodological issues related to the concepts of child and adult perspectives during mealtime, and to the children's participation in research. Through the paper, we take part in a critical discussion of the dichotomization of child and adult perspectives (Lee, 2001; Spyrou, 2011; Wyness, 2013). Instead, we suggest to approach perspectives as something that is created through situated interactions between human and nonhuman elements. We argue that perspectives could be studied as constructed from a net of human, material, and discursive elements. In doing so, we also question children's participation in research as a source for production of knowledge that is more authentic than that produced by adults (Gallacher & Gallagher, 2008; Lomax, 2012). Hence, the kindergarten mealtime will provide the arena for discussing methodological issues that are relevant beyond studies on food and meals. more...

Background and Aims Epidemiological studies have shown that subnormal levels of vitamin D (25[OH]D) are associated with a more aggravated clinical course of ulcerative colitis [UC]. Despite an increased focus on the therapeutic importance of vitamin D and vitamin D receptor [VDR] signalling, the mechanisms underlying the effects of the vitamin D–VDR axis on UC remain elusive. Therefore, we aimed to investigate whether exposure to active vitamin D (1,25[OH]2D3/VDR) signalling in human organoids could influence the maintenance of the colonic epithelium. Methods Intestinal VDR expression was studied by immunohistochemistry, RNA expression arrays, and single-cell RNA sequencing of colonic biopsy specimens obtained from patients with UC and healthy individuals. To characterise the functional and transcriptional effects of 1,25[OH]2D3, we used patient-derived colonic organoids. The dependency of VDR was assessed by knocking out the receptor with CRISPR/Cas9. Results Our results suggest that 1,25[OH]2D3/VDR stimulation supports differentiation of the colonic epithelium and that impaired 1,25[OH]2D3/VDR signalling thereby may compromise the structure of the intestinal epithelial barrier, leading to flares of UC. Furthermore, a transcriptional response to VDR activity was observed primarily in fully differentiated cells at the top of the colonic crypt, and this response was reduced during flares of UC. Conclusions We identified an important role of vitamin D signalling in supporting differentiated cell states in the human colonic epithelium, and thereby maintenance of the intestinal barrier integrity. This makes the vitamin D–VDR signalling axis an interesting target for therapeutic efforts to achieve and maintain remission in patients with UC. [ABSTRACT FROM AUTHOR] more...

Background: Eosinophilic oesophagitis (EoE) is a chronic immune-mediated disease. In Denmark, the budesonide orodispersible tablet (BOT) is recommended as a second-line treatment for proton pump inhibitor-refractory EoE patients. Aims: To evaluate the effectiveness of treatment with BOT in adult EoE patients in a population-based setting in Denmark. Methods: This was a retrospective, registry-based, DanEoE cohort study of all 76 adult EoE patients treated with BOT and diagnosed between 2007 and 2021 in the North Denmark Region. After medical record revision, the EoE diagnosis was defined according to the AGREE consensus. Symptomatic response was based on the information found in the patients' medical reports and histologic remission was defined as <15 eosinophils per high-power field (eos/hpf). Results: Histologic remission was achieved in 89% of the patients treated with BOT who underwent histologic evaluation. Clinicohistologic remission was achieved in 71% of the patients who underwent both symptomatic and histologic evaluation. Despite histologic remission, 18% of patients still experienced symptoms. Non-responders were found in 7% of the patients. Complications were rare, with dilation of strictures performed in 7% and food bolus obstruction (FBO) occurring in 3%. Discontinuation of the treatment due to unacceptable side effects was observed in 11% of the treated patients. Conclusions: Treatment with BOT effectively induced histologic remission in most of the EoE patients. Despite achieving histologic remission, approximately 1/5 of the patients were still symptomatic. Complications were rare. In non-responders and those with unacceptable side effects, alternative treatment options such as biologic agents might be needed. [ABSTRACT FROM AUTHOR] more...

Neurodegenerative diseases constitute a global health issue and a major economic burden. They significantly impair both cognitive and motor functions, and their prevalence is expected to rise due to ageing societies and continuous population growth. Conventional therapies provide symptomatic relief, nevertheless, disease-modifying treatments that reduce or halt neuron death and malfunction are still largely unavailable. Amongst the common hallmarks of neurodegenerative diseases are protein aggregation, oxidative stress, neuroinflammation and mitochondrial dysfunction. Transcription factor nuclear factor-erythroid 2-related factor 2 (NRF2) constitutes a central regulator of cellular defense mechanisms, including the regulation of antioxidant, antiinflammatory and mitochondrial pathways, making it a highly attractive therapeutic target for disease modification in neurodegenerative disorders. Here, we describe the role of NRF2 in the common hallmarks of neurodegeneration, review the current pharmacological interventions and their challenges in activating the NRF2 pathway, and present alternative therapeutic approaches for disease modification. [ABSTRACT FROM AUTHOR] more...