Search

The aim was to develop a Quality Index Method (QIM) scheme for fresh tilapia fillets and evaluate the scheme in a shelf life study. Farmed tilapia fillets were stored at 1 and −1°C for up to 20 days, and changes during storage were observed with sensory evaluation using the QIM scheme and General Descriptive Analysis (GDA), total viable counts (TVC), and hydrogen sulphide (H2S) producing bacteria. The maximum shelf life of fillets stored at1 and −1°C was determined with GDA and microbial counts as 16 and 19 days, respectively. A high correlation of R2 = 0.943 between Quality Index (QI) and storage time at 1C° was found to be 0.843 at −1°C. At the end of shelf life, the QI was 5.3 and 6.5 out of a maximum score of 15 for fillets stored at 1 and −1°C, respectively. Total viable counts and H2S-producing bacteria showed an increasing trend during storage time for both groups. At the end of shelf life, TVC was log 7 cfu/g in the flesh of both groups, with H2S producing bacteria constituting a higher proportion...

Low field Nuclear Magnetic Resonance was used to evaluate the effect of salt and modified atmosphere packaging (MAP) on cod loins during superchilled storage. Transversal and longitudinal proton relaxation times of the cod loins were measured with Carr–Purcell–Meiboom–Gill (CPMG) and Inversion Recovery (IR) pulse sequences respectively. The relaxation parameters reflected the observed differences in the muscle caused by variation in salt concentration, the choice of salting method (brining or brine injection) and packaging (air or MAP), as well as superchilled storage temperature and storage time. Significant correlations were found between the NMR parameters and parameters describing the water dynamics of the muscle (moisture and salt content, water holding capacity, drip and cooking yield), as well as muscle pH and counts of H2S-producing bacteria in chosen sample groups. The study showed the possibility of using low field NMR to indicate fish quality deterioration, when the spoilage mechanisms affect the water properties and muscle structure.

Farmed arctic char were divided into two groups after slaughtering. One group (T1) was stored in ice up to 18 d and the other (T2) was stored at 18°C for 24 h (temperature increased from 3°C up to 12°C), then iced and stored up to 18 d. Changes during storage were observed with sensory evaluation using the Quality Index Method (QIM) and Quantitative Descriptive Analysis (QDA), total viable counts (TVC), and hydrogen sulphide (H2S)-producing bacteria. A high correlation between Quality Index (QI) and storage time in ice was found. Storage time could be predicted within ± 1.3 d. The maximum shelf life, determined with QDA and microbial counts, was 17 and 15 d, respectively, for iced (T1) and temperature-abused (T2) arctic char. At the end of shelf life, TVC was 105–106 CFU/g in the flesh of both groups, with H2S producing bacteria constituting a higher proportion of TVC in T2.

To meet modern consumer demands, it is important to be able to offer “ready-to-use” desalted cod in consumer packs with sufficient keeping quality at chill temperatures. The aim of this work was to investigate the effect of potassium sorbate, citric acid, and modified atmosphere packaging (MAP) in varying combinations on the keeping quality of desalted cod fillets in consumer packs. After desalting, cod fillets were packed in trays and stored at 1.0 ± 0.2 °C for up to 33 d. The fillets were packed in open bags, in modified atmosphere (MA) (CO2/N2/O2:75/ 20/5) only, or in MS following a potassium sorbate and/or a citric acid treatment. The rate of microbial growth was by far fastest in fillets in open bags. MAP alone decreased the growth rate considerably and still further decrease was obtained in MAP fillets treated with citric acid and/or sorbate solutions. The concurrent effect of these treatments was distinct. Quantitative descriptive analysis (QDA) was used to assess cooked samples. During the 1st d of storage, the samples were described by sweet and butter odor, salt taste, clammy and rubberlike texture, which became less evident with increasing storage time, but differed by groups and the least in samples treated with sorbate and citric acid/sorbate solutions. Sensory spoilage attributes and total volatile bases (TVB-N)/trimethylamine (TMA) measurements correlated well with microbial counts. Use of MAP increased the shelf life from 6 to 10 d to 18 to 24 d, MAP and citric acid to 24 to 28 d, while the addition of sorbate to MAP fillets extended the shelf life to at least 33 d.

The objective was to determine whether traditional quality indexes of fresh (unfrozen) fish like sensory analysis, bacterial counts and trimethylamine content could be applied to thawed whole cod, cod fillets and ocean perch fillets kept in ice. Freezing and short-term freezer storage (≤5 wk at -25 o C) had very little effect on bacterial counts. During longterm freezer storage (≥14 wk at -25 o C) total counts were reduced as well as counts of trimethylamine oxide-reducing bacteria in cod fillets but not in ocean perch fillets. When the thawed fish was unacceptable the trimethylamine was

The aim of this study was to use low-field nuclear magnetic resonance (NMR) and traditional chemical methods to investigate the physical and chemical differences in wild and farmed cod processed pre- and postrigor, and how these properties were affected by brine injection, brining, and freezing. In prerigor processed farmed or wild cod, brine injections followed by brining for 2 d, with brine concentrations up to 5.5% and 4%, respectively, were not sufficient to reach a muscle salt concentration of 2% as aimed for, while wild cod processed postrigor had sufficient salt uptake after the same processing. Low-field NMR gave valuable information about the differences in the muscle structure between wild and farmed cod as well as the state of the water in the muscle during brine injection, brining, and during rigor tension. Low-field NMR is, therefore, a valuable tool that can be used to optimize the salting and storing processes of lightly salted cod products from both wild and farmed cod. For farmed cod to be used in the production of lightly salted products further research is needed. Practical Application: Optimal processing of lightly salted cod products is important to the fish industry, due to an increasing market for this product in southern Europe. Farmed cod, which is seen as a potential steady raw material source for this production, differs considerably from its wild counterparts by having other chemical and physical muscle properties, such as lower water content and lower pH. With the processing procedures used today the farmed cod can, therefore, only be used in some of the products, where wild cod is currently used as raw material. It is, therefore, important that the processing of these products is optimized with regard to these differences in the raw material. This study gives a valuable contribution to further studies about optimal combinations of brine injections, brining, and freezing of pre- and postrigor processed farmed compared to wild cod.

Flavor characterization and quality of salt-cured and desalted cod (Gadus morhua) products was studied using sensory analysis and gas chromatography techniques. The products were produced in Iceland using two different processing methods (filleting and splitting) and three different salting procedures, i.e., the old single-step kench salting or a multistep procedure, and presalting (injection and brine salting or only brine salting), which was followed by kench salting. The main difference observed was between fillets and split fish, where the split fish was darker and had stronger flavor characteristics. Comparison of different salting procedures showed that the use of presalting improved the appearance of the salted products, which can be described as increased lightness and reduced yellowness of the products. In the same products, the intensity of curing flavors was milder, as described by sensory analysis and key aroma compounds. Derivatives from lipid and protein degradation contribute to the characteristic flavor of the salted products.

The aim of these experiments was to evaluate the effect of brining, modified atmosphere packaging (MAP), and superchilling on the quality changes of cod loins as measured by microbial, sensory, and chemical analysis. Unbrined and brined (2.5 +/- 1.0% NaCl) cod loins were kept in styrofoam boxes (air) and under modified atmosphere (MA, CO(2)/O(2)/N(2): 50/5/45) at 0, -2, and -3.6 degrees C. Samples were examined over a 4-wk period. Total viable psychrotrophic counts and counts of H(2)S-producing bacteria reached higher numbers in the air-packed brined fish at -2 and -3.6 degrees C than in comparable unbrined groups, being significantly different (P < 0.05) at the lower temperature. However, lower counts of these bacteria were obtained in the brined MAP fish than in comparable unbrined fish. Counts of Photobacterium phosphoreum increased most rapidly in air- and MA-packed loins kept at 0 degrees C. Lower counts were found at superchilled temperatures. According to sensory analysis the shelf life of unbrined air-packed loins was about 11 d at 0 degrees C and 14 to 15 d at -2 degrees C. The shelf life of MA-packed unbrined loins was about 14 to 15 d at 0 degrees C but 21 d at -2 degrees C. Thus, synergism of combined superchilling (-2 degrees C) and MA led to a considerable shelf life increase for unbrined loins despite the fact that processing and packaging took place 4 to 5 d post-catch. The shelf life of air-packed brined loins at -2 degrees C was 12 to 15 d but only 13 d under MA. The same synergistic effect did therefore not apply to brined loins as with unbrined ones.

Background The bacteriology during storage of the North-Atlantic cod has been investigated for the past decades using conventional cultivation strategies which have generated large amount of information. This paper presents a study where both conventional cultivation and cultivation independent approaches were used to investigate the bacterial succession during storage of cod loins at chilled and superchilled temperatures. Results Unbrined (0.4% NaCl) and brined (2.5% NaCl) cod loins were stored at chilled (0°C) and superchilled (-2 and -3.6°C) temperatures in air or modified atmosphere (MA, % CO2/O2/N2: 49.0 ± 0.6/7.4 ± 0.2/43.7 ± 0.4). Discrepancy was observed between cultivation enumeration and culture independent methods where the former showed a general dominance of Pseudomonas spp. (up to 59%) while the latter showed a dominance of Photobacterium phosphoreum (up to 100%). Gas chromatography-mass spectrophotometry (GC-MC) showed that trimethylamine was the most abundant volatile in mid- and late storage periods. Terminal restriction polymorphism (t-RFLP) analysis showed that the relative abundance of P. phosphoreum increased with storage time. Conclusion The present study shows the bacteriological developments on lightly salted or non-salted cod loins during storage at superchilled temperatures. It furthermore confirms the importance of P. phosphoreum as a spoilage organism during storage of cod loins at low temperatures using molecular techniques. The methods used compensate each other, giving more detailed data on bacterial population developments during spoilage.

Pseudomonas spp. is a group of microorganisms commonly found in fish and other fresh foods and is involved in their spoilage process. The aim of this study was to develop a rapid and accurate quantitative assay for Pseudomonas spp. in fish using real-time PCR. The assay targets the carbamoyl phosphate synthase gene (carA) with SYBR green based real-time PCR. The selectivity of the assay was confirmed using 24 Pseudomonas strains and 55 non-pseudomonad strains. A linear quantification was established over seven orders of magnitude, from 40 - 4(7) copies reaction(-1). The assay was validated on cod samples collected during two shelf life trials and showed a high degree of correlation to the plate count method (rP = 0.891) where the difference between the methods was 0.04 log(10) CFU g(-1) on average. The study shows that it is possible to quantify accurately the specific spoilage organisms belonging to the genus Pseudomonas in fish using real-time PCR. The method takes less than 5 h from sampling to results. The short detection time of the method can provide the fish industry with an important tool for quality control and processing management.