34 results on '"Hangelbroek, Roland"'
Search Results
2. Impact of protein supplementation during endurance training on changes in skeletal muscle transcriptome
- Author
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Knuiman, Pim, Hangelbroek, Roland, Boekschoten, Mark, Hopman, Maria, and Mensink, Marco
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- 2020
- Full Text
- View/download PDF
3. No effect of 25-hydroxyvitamin D supplementation on the skeletal muscle transcriptome in vitamin D deficient frail older adults
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Hangelbroek, Roland W. J., Vaes, Anouk M. M., Boekschoten, Mark V., Verdijk, Lex B., Hooiveld, Guido J. E. J., van Loon, Luc J. C., de Groot, Lisette C. P. G. M., and Kersten, Sander
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- 2019
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4. Creatine Loading Does Not Preserve Muscle Mass or Strength During Leg Immobilization in Healthy, Young Males: A Randomized Controlled Trial
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Backx, Evelien M. P., Hangelbroek, Roland, Snijders, Tim, Verscheijden, Marie-Louise, Verdijk, Lex B., de Groot, Lisette C. P. G. M., and van Loon, Luc J. C.
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- 2017
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5. PLIS: A metabolomic response monitor to a lifestyle intervention study in older adults
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Bogaards, Fatih A., Gehrmann, Thies, Beekman, Marian, van den Akker, Erik Ben, van de Rest, Ondine, Hangelbroek, Roland W. J., Noordam, Raymond, Mooijaart, Simon P., de Groot, Lisette C. P. G. M., Reinders, Marcel J. T., and Slagboom, P. Eline
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Male ,Nutrition and Disease ,Biochemistry ,Voeding, Metabolisme en Genomica ,Voeding ,Voeding en Ziekte ,OT BVG/MNH/MDS ,Genetics ,Humans ,Insulin ,Life Science ,Obesity ,Life Style ,Molecular Biology ,Aged ,Nutrition ,VLAG ,Cholesterol, HDL ,lifestyle intervention ,bioinformatics ,response monitor ,metabolomics ,Metabolism and Genomics ,Nutritional Biology ,machine learning ,Metabolisme en Genomica ,Female ,Nutrition, Metabolism and Genomics ,healthy ageing ,Biomarkers ,Biotechnology - Abstract
The response to lifestyle intervention studies is often heterogeneous, especially in older adults. Subtle responses that may represent a health gain for individuals are not always detected by classical health variables, stressing the need for novel biomarkers that detect intermediate changes in metabolic, inflammatory, and immunity-related health. Here, our aim was to develop and validate a molecular multivariate biomarker maximally sensitive to the individual effect of a lifestyle intervention; the Personalized Lifestyle Intervention Status (PLIS). We used 1 H-NMR fasting blood metabolite measurements from before and after the 13-week combined physical and nutritional Growing Old TOgether (GOTO) lifestyle intervention study in combination with a fivefold cross-validation and a bootstrapping method to train a separate PLIS score for men and women. The PLIS scores consisted of 14 and four metabolites for females and males, respectively. Performance of the PLIS score in tracking health gain was illustrated by association of the sex-specific PLIS scores with several classical metabolic health markers, such as BMI, trunk fat%, fasting HDL cholesterol, and fasting insulin, the primary outcome of the GOTO study. We also showed that the baseline PLIS score indicated which participants respond positively to the intervention. Finally, we explored PLIS in an independent physical activity lifestyle intervention study, showing similar, albeit remarkably weaker, associations of PLIS with classical metabolic health markers. To conclude, we found that the sex-specific PLIS score was able to track the individual short-term metabolic health gain of the GOTO lifestyle intervention study. The methodology used to train the PLIS score potentially provides a useful instrument to track personal responses and predict the participant's health benefit in lifestyle interventions similar to the GOTO study.
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- 2022
6. Weight loss moderately affects the mixed meal challenge response of the plasma metabolome and transcriptome of peripheral blood mononuclear cells in abdominally obese subjects
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Fazelzadeh, Parastoo, Hangelbroek, Roland W. J., Joris, Peter J., Schalkwijk, Casper G., Esser, Diederik, Afman, Lydia, Hankemeier, Thomas, Jacobs, Doris M., Mihaleva, Velitchka V., Kersten, Sander, van Duynhoven, John, and Boekschoten, Mark V.
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- 2018
- Full Text
- View/download PDF
7. The impact of an intrinsic dried vegetable fibre on gut microbiota, bowel function and glucose homeostasis markers.
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Puhlmann, Marie-Luise, Jokela, Roosa, van Dongen, Katja, Bui, Nam, Hangelbroek, Roland, Smidt, Hauke, de Vos, Willem, Feskens, Edith, Puhlmann, Marie-Luise, Jokela, Roosa, van Dongen, Katja, Bui, Nam, Hangelbroek, Roland, Smidt, Hauke, de Vos, Willem, and Feskens, Edith
- Abstract
The study was a two-arm randomized, placebo-controlled, investigator-blinded parallel trial in 55 subjects at risk of type 2 diabetes. The Medical Ethical Review Committee Wageningen University (METC-WU nr. 17/25) approved this study, it was carried out in according to the Declaration of Helsinki and was registered at the ISRCTN registry (ISRCTN39985847). The treatment product was an intrinsic multifibre product consisting of dried chicory roots, which are rich in native inulin enclosed within plant cell-walls made of pectin, cellulose and hemi-cellulose. Participants were eligible if they were between 40 and 75 year old and had either a fasting blood glucose level between 5.0 and 5.6 mmol/L with a high risk to develop type 2 diabetes (T2D) later in life as assessed by a diabetes risk score ≥ 9, or a fasting blood glucose level of 5.6 and 6.9 mmol/L (classified as prediabetes by the American Diabetes Association). The study consisted of three weeks intervention where subjects consumed 30 g of the multifibre product (n=28) per day or an iso-caloric placebo (maltodextrin, n=27). This study period was preceded by a two-week run-in period at half-dose and followed by a two week washout. At baseline, after the two-week run-in, after the three-weeks study and after the two-week washout faecal samples were collected to determine changes in gut microbiota composition. Samples were directly frozen at home and stored in the freezer (-20°C) until transport in frozen form to the laboratory and subsequent storage at -80°C. Besides gut microbiota composition, we also assessed faecal short-chain fatty acids a well as bowel function by monitoring stool softness and stool frequency, type 2 diabetes biomarkers by measuring circulating short-chain fatty acids, fasting insulin, fasting glucose and HOMA-ir in plasma (taken at baseline and after three weeks of 30g product intake) as well as changes in the coefficient of variation by continuous glucose measurement during baseline, run-in
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- 2022
8. Relative Validity and Reliability of Isometric Lower Extremity Strength Assessment in Older Adults by Using a Handheld Dynamometer
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Grootswagers, Pol, Vaes, Anouk M.M., Hangelbroek, Roland, Tieland, Michael, van Loon, Luc J.C., de Groot, Lisette C.P.G.M., Grootswagers, Pol, Vaes, Anouk M.M., Hangelbroek, Roland, Tieland, Michael, van Loon, Luc J.C., and de Groot, Lisette C.P.G.M.
- Abstract
Background: Handheld dynamometry (HHD) is a practical alternative to traditional testing of lower extremity strength. However, its reliability and validity across different populations and settings are not clear. Hypothesis: We hypothesize that HHD is a valid and reliable device to assess lower extremity strength in a population of older adults. Study Design: Cross-sectional/cohort. Level of Evidence: Level 3. Methods: This study included 258 older adults (≥65 years). Isometric knee extension and flexion force were measured by 1 examiner, using an HHD (n = 222), including 3 repetitions to calculate within-day intrarater reliability. These measurements were repeated by the examiner in a subgroup (n = 23) to analyze intrarater reliability over a test-retest period of on average 8 weeks. In addition, HHD force measures were performed by a second examiner (n = 29) to analyze interrater reliability. In another subgroup (n = 77), isometric knee extension and flexion torque were measured by 1 examiner using both the HHD and Biodex System 4 to assess relative validity. Results: HHD and Biodex measurements were highly correlated and showed excellent concurrent validity. HHD systematically overestimated torque as compared with Biodex by 8 N·m on average. Same-day intrarater intraclass correlation coefficients (ICCs) ranged from 0.97 to 0.98. Interrater reliability ICCs ranged from 0.83 to 0.95. Conclusion: HHD represents a reliable and valid alternative to Biodex to rank individuals on leg strength, or to assess within-person changes in leg strength over time, because of the high validity and reliability. The HHD is less suited for absolute strength assessment because of significant systematic overestimations. Clinical Relevance: Clinicians are encouraged to use HHD to rank older adults on leg strength, or to assess within-person changes in leg strength over time, but not to compare readings with cut-offs or normative values.
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- 2022
9. PLIS: A metabolomic response monitor to a lifestyle intervention study in older adults
- Author
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Bogaards, Fatih A. (author), Gehrmann, Thies (author), Beekman, Marian (author), van den Akker, E.B. (author), van de Rest, Ondine (author), Hangelbroek, Roland W.J. (author), Noordam, Raymond (author), Mooijaart, Simon P. (author), Reinders, M.J.T. (author), Bogaards, Fatih A. (author), Gehrmann, Thies (author), Beekman, Marian (author), van den Akker, E.B. (author), van de Rest, Ondine (author), Hangelbroek, Roland W.J. (author), Noordam, Raymond (author), Mooijaart, Simon P. (author), and Reinders, M.J.T. (author)
- Abstract
The response to lifestyle intervention studies is often heterogeneous, especially in older adults. Subtle responses that may represent a health gain for individuals are not always detected by classical health variables, stressing the need for novel biomarkers that detect intermediate changes in metabolic, inflammatory, and immunity-related health. Here, our aim was to develop and validate a molecular multivariate biomarker maximally sensitive to the individual effect of a lifestyle intervention; the Personalized Lifestyle Intervention Status (PLIS). We used 1 H-NMR fasting blood metabolite measurements from before and after the 13-week combined physical and nutritional Growing Old TOgether (GOTO) lifestyle intervention study in combination with a fivefold cross-validation and a bootstrapping method to train a separate PLIS score for men and women. The PLIS scores consisted of 14 and four metabolites for females and males, respectively. Performance of the PLIS score in tracking health gain was illustrated by association of the sex-specific PLIS scores with several classical metabolic health markers, such as BMI, trunk fat%, fasting HDL cholesterol, and fasting insulin, the primary outcome of the GOTO study. We also showed that the baseline PLIS score indicated which participants respond positively to the intervention. Finally, we explored PLIS in an independent physical activity lifestyle intervention study, showing similar, albeit remarkably weaker, associations of PLIS with classical metabolic health markers. To conclude, we found that the sex-specific PLIS score was able to track the individual short-term metabolic health gain of the GOTO lifestyle intervention study. The methodology used to train the PLIS score potentially provides a useful instrument to track personal responses and predict the participant's health benefit in lifestyle interventions similar to the GOTO study., Pattern Recognition and Bioinformatics
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- 2022
- Full Text
- View/download PDF
10. Relative Validity and Reliability of Isometric Lower Extremity Strength Assessment in Older Adults by Using a Handheld Dynamometer
- Author
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Grootswagers, Pol, primary, Vaes, Anouk M. M., additional, Hangelbroek, Roland, additional, Tieland, Michael, additional, van Loon, Luc J. C., additional, and de Groot, Lisette C. P. G. M., additional
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- 2022
- Full Text
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11. Dried chicory root improves bowel function, benefits intestinal microbial trophic chains and increases faecal and circulating short chain fatty acids in subjects at risk for type 2 diabetes
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Puhlmann, Marie-Luise, primary, Jokela, Roosa, additional, van Dongen, Katja Catharina Wilhelmina, additional, Bui, Thi Phuong Nam, additional, Hangelbroek, Roland Willem Jan van, additional, Smidt, Hauke, additional, de Vos, Willem Meindert, additional, and Feskens, Edith Johanna Maria, additional
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- 2022
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12. Comparative Analysis of the Effects of Fish Oil and Fenofibrate on Plasma Metabolomic Profiles in Overweight and Obese Individuals
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Michielsen, Charlotte C.J.R., primary, Hangelbroek, Roland W.J., additional, Bragt, Marjolijn C.E., additional, Verheij, Elwin R., additional, Wopereis, Suzan, additional, Mensink, Ronald P., additional, and Afman, Lydia A., additional
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- 2021
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13. RNA sequencing reveals niche gene expression effects of beta-hydroxybutyrate in primary myotubes
- Author
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Ruppert, Philip MM, primary, Deng, Lei, additional, Hooiveld, Guido JEJ, additional, Hangelbroek, Roland WJ, additional, Zeigerer, Anja, additional, and Kersten, Sander, additional
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- 2021
- Full Text
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14. RNA sequencing reveals niche gene expression effects of beta-hydroxybutyrate in primary myotubes
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Ruppert, Philip M.M., Deng, Lei, Hooiveld, Guido J.E.J., Hangelbroek, Roland W.J., Zeigerer, Anja, Kersten, Sander, Ruppert, Philip M.M., Deng, Lei, Hooiveld, Guido J.E.J., Hangelbroek, Roland W.J., Zeigerer, Anja, and Kersten, Sander
- Abstract
Various forms of fasting and ketogenic diet have shown promise in (pre-)clinical studies to normalize body weight, improve metabolic health, and protect against disease. Recent studies suggest that β-hydroxybutyrate (βOHB), a fasting-characteristic ketone body, potentially acts as a signaling molecule mediating its beneficial effects via histone deacetylase inhibition. Here, we have investigated whether βOHB, in comparison to the well-established histone deacetylase inhibitor butyrate, influences cellular differentiation and gene expression. In various cell lines and primary cell types, millimolar concentrations of βOHB did not alter differentiation in vitro, as determined by gene expression and histological assessment, whereas equimolar concentrations of butyrate consistently impaired differentiation. RNA sequencing revealed that unlike butyrate, βOHB minimally impacted gene expression in primary adipocytes, macrophages, and hepatocytes. However, in myocytes, βOHB up-regulated genes involved in the TCA cycle and oxidative phosphorylation, while down-regulating genes belonging to cytokine and chemokine signal transduction. Overall, our data do not support the notion that βOHB serves as a powerful signaling molecule regulating gene expression but suggest that βOHB may act as a niche signaling molecule in myocytes.
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- 2021
15. Comparative transcriptome analysis of human skeletal muscle in response to cold acclimation and exercise training in human volunteers
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Nascimento, Emmani B.M., Hangelbroek, Roland W.J., Hooiveld, Guido J.E.J., Hoeks, Joris, van Marken Lichtenbelt, Wouter D., Hesselink, Matthijs H.C., Schrauwen, Patrick, Kersten, Sander, Nascimento, Emmani B.M., Hangelbroek, Roland W.J., Hooiveld, Guido J.E.J., Hoeks, Joris, van Marken Lichtenbelt, Wouter D., Hesselink, Matthijs H.C., Schrauwen, Patrick, and Kersten, Sander
- Abstract
This SuperSeries is composed of the SubSeries listed below. Overall design: Refer to individual Series, This SuperSeries is composed of the SubSeries listed below. Overall design: Refer to individual Series
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- 2020
16. Comparative transcriptome analysis of human skeletal muscle in response to cold acclimation and exercise training in human volunteers. [A294]
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Nascimento, Emmani B.M., Hangelbroek, Roland W.J., Hooiveld, Guido J.E.J., Hoeks, Joris, van Marken Lichtenbelt, Wouter D., Hesselink, Matthijs H.C., Schrauwen, Patrick, Kersten, Sander, Nascimento, Emmani B.M., Hangelbroek, Roland W.J., Hooiveld, Guido J.E.J., Hoeks, Joris, van Marken Lichtenbelt, Wouter D., Hesselink, Matthijs H.C., Schrauwen, Patrick, and Kersten, Sander
- Abstract
Background: Cold acclimation and exercise training were previously shown to increase peripheral insulin sensitivity in human volunteers with type 2 diabetes. Although cold is a potent activator of brown adipose tissue, the increase in peripheral insulin sensitivity by cold is largely mediated by events occurring in skeletal muscle and at least partly involves GLUT4 translocation, as is also observed for exercise training. Results: To investigate if cold acclimation and exercise training overlap in the molecular adaptive response in skeletal muscle, we performed transcriptomics analysis on vastus lateralis muscle collected from human subjects before and after 10 days of cold acclimation, as well as before and after a 12-week exercise training intervention. Methods: Cold acclimation altered the expression of 756 genes (422 up, 334 down, P<0.01), while exercise training altered the expression of 665 genes (444 up, 221 down, P<0.01). Principal Component Analysis, Venn diagram, similarity analysis and Rank–rank Hypergeometric Overlap all indicated significant overlap between cold acclimation and exercise training in upregulated genes, but not in downregulated genes. Overlapping gene regulation was especially evident for genes and pathways associated with extracellular matrix remodeling. Interestingly, the genes most highly induced by cold acclimation were involved in contraction and in signal transduction between nerve and muscle cells, while no significant changes were observed in genes and pathways related to insulin signaling or glucose metabolism. Conclusions: Overall, our results indicate that cold acclimation and exercise training have overlapping effects on gene expression in human skeletal muscle, but strikingly these overlapping genes are designated to pathways related to cell remodeling rather than metabolic pathways. Overall design: Microarray analysis was performed on a muscle biopsies taken from overweight middle-aged male subjects before and after a 12 week, Background: Cold acclimation and exercise training were previously shown to increase peripheral insulin sensitivity in human volunteers with type 2 diabetes. Although cold is a potent activator of brown adipose tissue, the increase in peripheral insulin sensitivity by cold is largely mediated by events occurring in skeletal muscle and at least partly involves GLUT4 translocation, as is also observed for exercise training. Results: To investigate if cold acclimation and exercise training overlap in the molecular adaptive response in skeletal muscle, we performed transcriptomics analysis on vastus lateralis muscle collected from human subjects before and after 10 days of cold acclimation, as well as before and after a 12-week exercise training intervention. Methods: Cold acclimation altered the expression of 756 genes (422 up, 334 down, P<0.01), while exercise training altered the expression of 665 genes (444 up, 221 down, P<0.01). Principal Component Analysis, Venn diagram, similarity analysis and Rank–rank Hypergeometric Overlap all indicated significant overlap between cold acclimation and exercise training in upregulated genes, but not in downregulated genes. Overlapping gene regulation was especially evident for genes and pathways associated with extracellular matrix remodeling. Interestingly, the genes most highly induced by cold acclimation were involved in contraction and in signal transduction between nerve and muscle cells, while no significant changes were observed in genes and pathways related to insulin signaling or glucose metabolism. Conclusions: Overall, our results indicate that cold acclimation and exercise training have overlapping effects on gene expression in human skeletal muscle, but strikingly these overlapping genes are designated to pathways related to cell remodeling rather than metabolic pathways. Overall design: Microarray analysis was performed on a muscle biopsies taken from overweight middle-aged male subjects before and after a 12 week
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- 2020
17. Transcriptomic analysis reveals niche gene expression effects of beta-hydroxybutyrate in primary myotubes
- Author
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Ruppert, Philip M. M., primary, Hooiveld, Guido J. E. J., additional, Hangelbroek, Roland W. J., additional, Zeigerer, Anja, additional, and Kersten, Sander, additional
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- 2021
- Full Text
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18. Nutritional Supplement Use by Dutch Elite and Sub-Elite Athletes: Does Receiving Dietary Counseling Make a Difference?
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Wardenaar, Floris C., Ceelen, Ingrid J. M., Van Dijk, Jan-Willem, Hangelbroek, Roland W. J., Van Roy, Lore, Van der Pouw, Britte, De Vries, Jeanne H. M., Mensink, Marco, and Witkamp, Renger F.
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CAFFEINE ,VITAMIN therapy ,THERAPEUTIC use of minerals ,CONFIDENCE intervals ,DIETARY supplements ,NUTRITION counseling ,PROBABILITY theory ,QUESTIONNAIRES ,REGRESSION analysis ,T-test (Statistics) ,ERGOGENIC aids ,ELITE athletes ,DISEASE prevalence ,CROSS-sectional method ,SPORTS drinks ,DATA analysis software ,DESCRIPTIVE statistics ,MANN Whitney U Test ,THERAPEUTICS - Abstract
The use of nutritional supplements is highly prevalent among athletes. In this cross-sectional study, we assessed the prevalence of nutritional supplement use by a large group of Dutch competitive athletes in relation to dietary counseling. A total of 778 athletes (407 males and 371 females) completed a web-based questionnaire about the use of nutritional supplements. Log-binomial regression models were applied to estimate crude and adjusted prevalence ratios (PR) for the use of individual nutritional supplements in athletes receiving dietary counseling as compared with athletes not receiving dietary counseling. Of the athletes, 97.2% had used nutritional supplements at some time during their sports career, whereas 84.7% indicated having used supplements during the last 4 weeks. The top ranked supplements used over the last 4 weeks from dietary supplements, sport nutrition products and ergogenic supplements were multivitamin and mineral preparations (42.9%), isotonic sports drinks (44.1%) and caffeine (13.0%). After adjustment for elite status, age, and weekly exercise duration, dietary counseling was associated with a higher prevalence of the use of vitamin D, recovery drinks, energy bars, isotonic drinks with protein, dextrose, beta-alanine, and sodium bicarbonate. In contrast, dietary counseling was inversely associated with the use of combivitamins, calcium, vitamin E, vitamin B2, retinol, energy drinks and BCAA and other amino acids. In conclusion, almost all athletes had used nutritional supplements at some time during their athletic career. Receiving dietary counseling seemed to result in better-informed choices with respect to the use of nutritional supplements related to performance, recovery, and health. [ABSTRACT FROM AUTHOR]
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- 2017
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19. Comparative Analysis of the Effects of Fish Oil and Fenofibrate on Plasma Metabolomic Profiles in Overweight and Obese Individuals.
- Author
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Michielsen, Charlotte C.J.R., Hangelbroek, Roland W.J., Bragt, Marjolijn C.E., Verheij, Elwin R., Wopereis, Suzan, Mensink, Ronald P., and Afman, Lydia A.
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- 2022
- Full Text
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20. No effect of 25-hydroxyvitamin D supplementation on the skeletal muscle transcriptome in vitamin D deficient frail older adults
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Hangelbroek, Roland W.J., Vaes, Anouk M.M., Boekschoten, Mark V., Verdijk, Lex B., Hooiveld, Guido J.E.J., Loon, Luc J.C., van, Groot, Lisette C.P.G.M., De, Kersten, Sander, Hangelbroek, Roland W.J., Vaes, Anouk M.M., Boekschoten, Mark V., Verdijk, Lex B., Hooiveld, Guido J.E.J., Loon, Luc J.C., van, Groot, Lisette C.P.G.M., De, and Kersten, Sander
- Abstract
Objective: Vitamin D deficiency is common among older adults and has been linked to muscle weakness. Vitamin D supplementation has been proposed as a strategy to improve muscle function in older adults. The aim of this study was to investigate the effect of calcifediol (25-hydroxycholecalciferol) on whole genome gene expression in skeletal muscle of vitamin D deficient frail older adults. Methods: A double-blind placebo-controlled trial was conducted in vitamin D deficient frail older adults (aged above 65), characterized by blood 25-hydroxycholecalciferol concentrations between 20 and 50 nmol/L. Subjects were randomized across the placebo group and the calcifediol group (10 μg per day). Muscle biopsies were obtained before and after 6 months of calcifediol (n = 10) or placebo (n = 12) supplementation and subjected to whole genome gene expression profiling using Affymetrix HuGene 2.1ST arrays. Results: Expression of the vitamin D receptor gene was virtually undetectable in human skeletal muscle biopsies, with Ct values exceeding 30. Blood 25-hydroxycholecalciferol levels were significantly higher after calcifediol supplementation (87.3 ± 20.6 nmol/L) than after placebo (43.8 ± 14.1 nmol/L). No significant difference between treatment groups was observed on strength outcomes. The whole transcriptome effects of calcifediol and placebo were very weak, as indicated by the fact that correcting for multiple testing using false discovery rate did not yield any differentially expressed genes using any reasonable cut-offs (all q-values ~ 1). P-values were uniformly distributed across all genes, suggesting that low p-values are likely to be false positives. Partial least squares-discriminant analysis and principle component analysis was unable to separate treatment groups. Conclusion: Calcifediol supplementation did not significantly affect the skeletal muscle transcriptome in frail older adults. Our findings indicate that vitamin D supplementation has no effects on skelet
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- 2019
21. Disentangling the Effects of Monounsaturated Fatty Acids from Other Components of a Mediterranean Diet on Serum Metabolite Profiles: A Randomized Fully Controlled Dietary Intervention in Healthy Subjects at Risk of the Metabolic Syndrome
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Michielsen, Charlotte C. J. R., primary, Hangelbroek, Roland W.J., additional, Feskens, Edith J. M., additional, and Afman, Lydia A., additional
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- 2019
- Full Text
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22. Plasma cytokine responses to resistance exercise with different nutrient availability on a concurrent exercise day in trained healthy males
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Knuiman, Pim, Hopman, M.T.E., Hangelbroek, Roland, Mensink, M., Knuiman, Pim, Hopman, M.T.E., Hangelbroek, Roland, and Mensink, M.
- Abstract
Contains fulltext : 192632.pdf (publisher's version ) (Open Access)
- Published
- 2018
23. Molecular assessment of muscle health and function : The effect of age, nutrition and physical activity on the human muscle transcriptome and metabolom
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Hangelbroek, Roland W.J., Wageningen University, A.H. Kersten, C.P.G.M. de Groot, and M.V. Boekschoten
- Subjects
leeftijd ,Humane Voeding & Gezondheid ,lichamelijke activiteit ,fosfocreatine ,vitamine d ,physical activity ,vitamin d ,metabolomes ,elderly ,spieren ,metabolomen ,creatine ,nutrition ,atrofie ,age ,atrophy ,transcriptomes ,ouderen ,voeding ,muscles ,transcriptomen ,phosphocreatine ,VLAG ,Human Nutrition & Health - Abstract
Prolonged lifespan and decreased fertility will lead to an increased proportion of older adults in the world population (population aging). An important strategy to deal with population aging has been to promote healthy aging; not only to prevent mounting health care costs, but also to maintain independence and quality of life of older populations for as long as possible. Close to the opposite of the healthy aging is frailty. A major component of (physical) frailty is sarcopenia: age-related loss of muscle mass. Decreased muscle size and strength has been associated with a wide variety of negative health outcomes, including increased risk of hospitalization, physical disability and even death. Therefore, maintaining muscle size and strength is very important for healthy aging. Nutrition and physical activity are possible strategies to maintain or even improve muscle function with age. The effect of nutrition, age, frailty and physical activity on the function of skeletal muscle is complex. A better understanding of the molecular mechanisms involved can provide new insights in potential strategies to maintain muscle function over the life course. This thesis aims to investigate these mechanisms and processes that underlie the effects of age, frailty and physical activity by leveraging the sensitivity and comprehensiveness of transcriptomics and metabolomics. Chapter 2 and 3 describe the effects of age, frailty and resistance-type exercise training on the skeletal muscle transcriptome and metabolome. Both the transcriptome and metabolome show significant differences between frail and healthy older adults. These differences are similar to the differneces between healthy young men and healthy older adults, suggesting that frailty presents itself as a more pronounced form of aging, somewhat independent of chronological age. These age and frailty related differences in the transcriptome are partially reversed by resistance-type exercise training, in accordance with the observed improvement in muscle strength. Regression analysis revealed that the protocadherin gamma gene cluster may be important to skeletal muscle function. Protocadherin gamma is involved in axon guidance and may be upregulated due to the denervation-reinnervation cycles observed in skeletal muscle of older individuals. The metabolome suggested that resistance-type exercise training led to a decrease in branched-chain amino acid oxidation, as shown by a decrease in amino acid derived carnitines. Lastly, the blood metabolome showed little agreement with the metabolome in skeletal muscle, indicating that blood is a poor read-out of muscle metabolism. We assessed the effect of knee immobilization with creatine supplementation or placebo on the skeletal muscle transcriptome and metabolome in chapter 4. Knee immobilization caused muscle mass loss and strength loss in all participants, with no differences between creatine and placebo groups. Knee immobilization appeared to induce the HDAC4-myogenin axis, which is primarily associated with denervation and motor neuron diseases. The metabolome showed changes consistent with the decreased expression of energy metabolism genes. While acyl-carnitine levels tended to decrease with knee immobilization, one branched-chain amino acid-derived acyl carnitine was increased after knee immobilization, suggesting increased amino acid oxidation. Vitamin D deficiency is common among older adults and has been linked to muscle weakness. Vitamin D supplementation has been proposed as a strategy to improve muscle function among older populations. In chapter 5, supplementation with vitamin D (calcifediol, 25(OH)D) is investigated as nutritional strategy to improve muscle function among frail older adults. However, we observed no effect of vitamin D on the muscle transcriptome. These findings indicate the effects of vitamin D supplementation on skeletal muscle may be either absent, weak, or limited to a small subset of muscle cells. Transcriptomic changes due to different forms of muscle disuse are compared in chapter 6 (primarily knee immobilization and bed rest). The goal was to determine the similarities and differences among various causes of muscle atrophy in humans (primarily muscle disuse). Both knee immobilization and bed rest led to significant changes in the muscle transcriptome. However, the overlap in significantly changed genes was relatively small. Knee immobilization was characterized by ubiquitin-mediated proteolysis and induction of the HDAC4/Myogenin axis, whereas bed rest revealed increased expression of genes of the immune system and increased expression of lysosomal genes. Knee immobilization showed the highest similarity with age and frailty-related transcriptomic changes. This finding suggests that knee immobilization may be the most suitable form of disuse atrophy to assess the effectiveness of strategies to prevent age-related muscle loss in humans. The transcriptome and metabolome are incredibly useful tools in describing the wide array of biological systems within skeletal muscle. These systems can be modulated using physical activity (or lack thereof) as well as nutrition. This thesis describes some of these processes and highlights several unexplored genes and metabolites that may be important for maintaining or even optimizing muscle function. In the future, it may be possible to optimize both exercise and nutrition for each individual using these techniques; or even better, cheaper and less invasive alternatives.
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- 2017
24. Attenuated strength gains during prolonged resistance exercise training in older adults with high inflammatory status
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Hangelbroek, Roland W.J., primary, Knuiman, Pim, additional, Tieland, Michael, additional, and de Groot, Lisette C.P.G.M., additional
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- 2018
- Full Text
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25. Plasma cytokine responses to resistance exercise with different nutrient availability on a concurrent exercise day in trained healthy males
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Knuiman, Pim, primary, Hopman, Maria T. E., additional, Hangelbroek, Roland, additional, and Mensink, Marco, additional
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- 2018
- Full Text
- View/download PDF
26. Molecular assessment of muscle health and function : The effect of age, nutrition and physical activity on the human muscle transcriptome and metabolom
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Kersten, A.H., de Groot, C.P.G.M., Boekschoten, M.V., Hangelbroek, Roland W.J., Kersten, A.H., de Groot, C.P.G.M., Boekschoten, M.V., and Hangelbroek, Roland W.J.
- Abstract
Prolonged lifespan and decreased fertility will lead to an increased proportion of older adults in the world population (population aging). An important strategy to deal with population aging has been to promote healthy aging; not only to prevent mounting health care costs, but also to maintain independence and quality of life of older populations for as long as possible. Close to the opposite of the healthy aging is frailty. A major component of (physical) frailty is sarcopenia: age-related loss of muscle mass. Decreased muscle size and strength has been associated with a wide variety of negative health outcomes, including increased risk of hospitalization, physical disability and even death. Therefore, maintaining muscle size and strength is very important for healthy aging. Nutrition and physical activity are possible strategies to maintain or even improve muscle function with age. The effect of nutrition, age, frailty and physical activity on the function of skeletal muscle is complex. A better understanding of the molecular mechanisms involved can provide new insights in potential strategies to maintain muscle function over the life course. This thesis aims to investigate these mechanisms and processes that underlie the effects of age, frailty and physical activity by leveraging the sensitivity and comprehensiveness of transcriptomics and metabolomics. Chapter 2 and 3 describe the effects of age, frailty and resistance-type exercise training on the skeletal muscle transcriptome and metabolome. Both the transcriptome and metabolome show significant differences between frail and healthy older adults. These differences are similar to the differneces between healthy young men and healthy older adults, suggesting that frailty presents itself as a more pronounced form of aging, somewhat independent of chronological age. These age and frailty related differences in the transcriptome are partially reversed by resistance-type exercise training, in accordance with the ob
- Published
- 2017
27. Creatine Loading Does Not Preserve Muscle Mass or Strength During Leg Immobilization in Healthy, Young Males : A Randomized Controlled Trial
- Author
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Backx, Evelien M.P., Hangelbroek, Roland, Snijders, Tim, Verscheijden, Marie Louise, Verdijk, Lex B., de Groot, Lisette C.P.G.M., van Loon, Luc J.C., Backx, Evelien M.P., Hangelbroek, Roland, Snijders, Tim, Verscheijden, Marie Louise, Verdijk, Lex B., de Groot, Lisette C.P.G.M., and van Loon, Luc J.C.
- Abstract
Background: A short period of leg immobilization leads to rapid loss of muscle mass and strength. Creatine supplementation has been shown to increase lean body mass in active individuals and can be used to augment gains in muscle mass and strength during prolonged resistance-type exercise training. Objective: Our objective was to investigate whether creatine loading can attenuate the loss of muscle mass and strength during short-term leg immobilization. Methods: Healthy young men (n = 30; aged 23 ± 1 years; body mass index [BMI] 23.3 ± 0.5 kg/m−2) were randomly assigned to either a creatine or a placebo group. Subjects received placebo or creatine supplements (20 g/d) for 5 days before one leg was immobilized by means of a full-leg cast for 7 days. Muscle biopsies were taken before creatine loading, prior to and immediately after leg immobilization, and after 7 days of subsequent recovery. Quadriceps cross-sectional area (CSA) (computed tomography [CT] scan) and leg muscle strength (one-repetition maximum [1-RM] knee extension) were assessed before and immediately after immobilization and after 1 week of recovery. Data were analyzed using repeated measures analysis of variance (ANOVA). Data are presented consistently as mean ± standard error of the mean (SEM). Results: There was a significant overall increase in muscle total creatine content following the 5-day loading phase (p = 0.049), which appeared driven by an increase in the creatine group (from 90 ± 9 to 107 ± 4 mmol/kg−1 dry muscle) with no apparent change in the placebo group (from 88 ± 4 to 90 ± 3 mmol/kg−1; p = 0.066 for time × treatment interaction). Quadriceps muscle CSA had declined by 465 ± 59 and 425 ± 69 mm2 (p <0.01) in the creatine and placebo group, respectively, with no differences between groups (p = 0.76). Leg muscle strength decreased from 56 ± 4 to 53 ± 4 kg in the creatine and from 59 ± 3 to 53 ± 3 kg in the placebo group, with no differences between groups (p = 0.20). Muscle fiber size did
- Published
- 2017
28. Expression of protocadherin gamma in skeletal muscle tissue is associated with age and muscle weakness
- Author
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Hangelbroek, Roland W. J., Fazelzadeh, Parastoo, Tieland, Michael, Boekschoten, Mark V., Hooiveld, Guido J. E. J., van Duynhoven, John P. M., Timmons, James A., Verdijk, Lex B., de Groot, Lisette C. P. G. M., van Loon, Luc J. C., Müller, Michael, RS: NUTRIM - HB/BW section A, RS: NUTRIM - R3 - Chronic inflammatory disease and wasting, and Nutrition and Movement Sciences
- Subjects
Male ,Aging ,Biopsy ,Cadherin Related Proteins ,Gene Expression ,Skeletal muscle ,Models, Biological ,Humans ,Muscle Strength ,Muscle, Skeletal ,Transcriptomics ,Exercise ,Aged ,Aged, 80 and over ,Muscle Weakness ,Frailty ,Gene Expression Profiling ,Age Factors ,Resistance Training ,Original Articles ,Cadherins ,Healthy Volunteers ,Ageing ,Original Article ,Female ,Transcriptome - Abstract
BACKGROUND: The skeletal muscle system plays an important role in the independence of older adults. In this study we examine differences in the skeletal muscle transcriptome between healthy young and older subjects and (pre-)frail older adults. Additionally, we examine the effect of resistance-type exercise training on the muscle transcriptome in healthy older subjects and (pre-)frail older adults.METHODS: Baseline transcriptome profiles were measured in muscle biopsies collected from 53 young, 73 healthy older subjects, and 61 frail older subjects. Follow-up samples from these frail older subjects (31 samples) and healthy older subjects (41 samples) were collected after 6 months of progressive resistance-type exercise training. Frail older subjects trained twice per week and the healthy older subjects trained three times per week.RESULTS: At baseline genes related to mitochondrial function and energy metabolism were differentially expressed between older and young subjects, as well as between healthy and frail older subjects. Three hundred seven genes were differentially expressed after training in both groups. Training affected expression levels of genes related to extracellular matrix, glucose metabolism ,and vascularization. Expression of genes that were modulated by exercise training was indicative of muscle strength at baseline. Genes that strongly correlated with strength belonged to the protocadherin gamma gene cluster (r = -0.73).CONCLUSION: Our data suggest significant remaining plasticity of ageing skeletal muscle to adapt to resistance-type exercise training. Some age-related changes in skeletal muscle gene expression appear to be partially reversed by prolonged resistance-type exercise training. The protocadherin gamma gene cluster may be related to muscle denervation and re-innervation in ageing muscle.
- Published
- 2016
29. The Muscle Metabolome Differs between Healthy and Frail Older Adults
- Author
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Fazelzadeh, Parastoo, primary, Hangelbroek, Roland W. J., additional, Tieland, Michael, additional, de Groot, Lisette C. P. G. M., additional, Verdijk, Lex B., additional, van Loon, Luc J. C., additional, Smilde, Age K., additional, Alves, Rodrigo D. A. M., additional, Vervoort, Jacques, additional, Müller, Michael, additional, van Duynhoven, John P. M., additional, and Boekschoten, Mark V., additional
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- 2016
- Full Text
- View/download PDF
30. Effects of acute exercise on gene expression in exercising and non-exercising human skeletal muscle
- Author
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Catoire, Milene, Mensink, Marco, Boekschoten, Mark, Hangelbroek, Roland, Muller, Michael, Schrauwen, Patricht, Kersten, Sander, Catoire, Milene, Mensink, Marco, Boekschoten, Mark, Hangelbroek, Roland, Muller, Michael, Schrauwen, Patricht, and Kersten, Sander
- Abstract
Background: Exercising is know to have an effect on exercising skeletal muscle, but unkown is the effect on non-exercising skeletal muscle. Gene expression changes in the non-exercising skeletal muscle would point to a signalling role of skeletal muscle, Background: Exercising is know to have an effect on exercising skeletal muscle, but unkown is the effect on non-exercising skeletal muscle. Gene expression changes in the non-exercising skeletal muscle would point to a signalling role of skeletal muscle
- Published
- 2012
31. Pronounced Effects of Acute Endurance Exercise on Gene Expression in Resting and Exercising Human Skeletal Muscle
- Author
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Catoire, Milène, primary, Mensink, Marco, additional, Boekschoten, Mark V., additional, Hangelbroek, Roland, additional, Müller, Michael, additional, Schrauwen, Patrick, additional, and Kersten, Sander, additional
- Published
- 2012
- Full Text
- View/download PDF
32. Molecular assessment of muscle health and function : The effect of age, nutrition and physical activity on the human muscle transcriptome and metabolom
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Hangelbroek, Roland W.J., primary
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33. PLIS: A metabolomic response monitor to a lifestyle intervention study in older adults.
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Bogaards FA, Gehrmann T, Beekman M, van den Akker EB, van de Rest O, Hangelbroek RWJ, Noordam R, Mooijaart SP, de Groot LCPGM, Reinders MJT, and Slagboom PE
- Subjects
- Aged, Biomarkers, Cholesterol, HDL, Female, Humans, Insulin, Male, Life Style, Obesity
- Abstract
The response to lifestyle intervention studies is often heterogeneous, especially in older adults. Subtle responses that may represent a health gain for individuals are not always detected by classical health variables, stressing the need for novel biomarkers that detect intermediate changes in metabolic, inflammatory, and immunity-related health. Here, our aim was to develop and validate a molecular multivariate biomarker maximally sensitive to the individual effect of a lifestyle intervention; the Personalized Lifestyle Intervention Status (PLIS). We used
1 H-NMR fasting blood metabolite measurements from before and after the 13-week combined physical and nutritional Growing Old TOgether (GOTO) lifestyle intervention study in combination with a fivefold cross-validation and a bootstrapping method to train a separate PLIS score for men and women. The PLIS scores consisted of 14 and four metabolites for females and males, respectively. Performance of the PLIS score in tracking health gain was illustrated by association of the sex-specific PLIS scores with several classical metabolic health markers, such as BMI, trunk fat%, fasting HDL cholesterol, and fasting insulin, the primary outcome of the GOTO study. We also showed that the baseline PLIS score indicated which participants respond positively to the intervention. Finally, we explored PLIS in an independent physical activity lifestyle intervention study, showing similar, albeit remarkably weaker, associations of PLIS with classical metabolic health markers. To conclude, we found that the sex-specific PLIS score was able to track the individual short-term metabolic health gain of the GOTO lifestyle intervention study. The methodology used to train the PLIS score potentially provides a useful instrument to track personal responses and predict the participant's health benefit in lifestyle interventions similar to the GOTO study., (© 2022 The Authors. The FASEB Journal published by Wiley Periodicals LLC on behalf of Federation of American Societies for Experimental Biology.)- Published
- 2022
- Full Text
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34. Expression of protocadherin gamma in skeletal muscle tissue is associated with age and muscle weakness.
- Author
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Hangelbroek RW, Fazelzadeh P, Tieland M, Boekschoten MV, Hooiveld GJ, van Duynhoven JP, Timmons JA, Verdijk LB, de Groot LC, van Loon LJ, and Müller M
- Subjects
- Age Factors, Aged, Aged, 80 and over, Aging metabolism, Biopsy, Cadherin Related Proteins, Cadherins metabolism, Exercise, Female, Gene Expression Profiling, Healthy Volunteers, Humans, Male, Models, Biological, Muscle Strength genetics, Muscle Weakness metabolism, Muscle Weakness pathology, Muscle Weakness physiopathology, Muscle, Skeletal pathology, Muscle, Skeletal physiopathology, Resistance Training, Transcriptome, Aging genetics, Cadherins genetics, Gene Expression, Muscle Weakness genetics, Muscle, Skeletal metabolism
- Abstract
Background: The skeletal muscle system plays an important role in the independence of older adults. In this study we examine differences in the skeletal muscle transcriptome between healthy young and older subjects and (pre-)frail older adults. Additionally, we examine the effect of resistance-type exercise training on the muscle transcriptome in healthy older subjects and (pre-)frail older adults., Methods: Baseline transcriptome profiles were measured in muscle biopsies collected from 53 young, 73 healthy older subjects, and 61 frail older subjects. Follow-up samples from these frail older subjects (31 samples) and healthy older subjects (41 samples) were collected after 6 months of progressive resistance-type exercise training. Frail older subjects trained twice per week and the healthy older subjects trained three times per week., Results: At baseline genes related to mitochondrial function and energy metabolism were differentially expressed between older and young subjects, as well as between healthy and frail older subjects. Three hundred seven genes were differentially expressed after training in both groups. Training affected expression levels of genes related to extracellular matrix, glucose metabolism ,and vascularization. Expression of genes that were modulated by exercise training was indicative of muscle strength at baseline. Genes that strongly correlated with strength belonged to the protocadherin gamma gene cluster ( r = -0.73)., Conclusions: Our data suggest significant remaining plasticity of ageing skeletal muscle to adapt to resistance-type exercise training. Some age-related changes in skeletal muscle gene expression appear to be partially reversed by prolonged resistance-type exercise training. The protocadherin gamma gene cluster may be related to muscle denervation and re-innervation in ageing muscle.
- Published
- 2016
- Full Text
- View/download PDF
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