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Data presented here are associated with the article: “In-depth proteomic analysis of soybean ( Glycine max ) seeds during controlled deterioration treatment (CDT) reveals a shift in seed metabolism” (Min et al., 2017) [1] . Seed deterioration is one of the major problems, affecting the seed quality, viability, and vigor in a negative manner. Here, we display the gel-based and gel-free proteomic data, associated with the CDT in soybean seeds. The present data was obtained from 2-DE, shotgun proteomic analysis (label-free quantitative proteomic analysis) using Q-Exactive, and gene ontology analysis associated with CDT in soybean seeds (Min et al., 2017) [1] .

Seed aging is one of the major events, affecting the overall quality of agricultural seeds. To analyze the effect of seed aging, soybean seeds were exposed to controlled deterioration treatment (CDT) for 3 and 7 days, followed by their physiological, biochemical, and proteomic analyses. Seed proteins were subjected to protamine sulfate precipitation for the enrichment of low-abundance proteins and utilized for proteome analysis. A total of 14 differential proteins were identified on 2-DE, whereas label-free quantification resulted in the identification of 1626 non-redundant proteins. Of these identified proteins, 146 showed significant changes in protein abundance, where 5 and 141 had increased and decreased abundances, respectively while 352 proteins were completely degraded during CDT. Gene ontology and KEGG analyses suggested the association of differential proteins with primary metabolism, ROS detoxification, translation elongation and initiation, protein folding, and proteolysis, where most, if not all, had decreased abundance during CDT. Western blotting confirmed reduced level of antioxidant enzymes (DHAR, APx1, MDAR, and SOD) upon CDT. This in-depth integrated study reveals a major downshift in seed metabolism upon CDT. Reported data here serve as a resource for its exploitation to metabolic engineering of seeds for multiple purposes, including increased seed viability, vigor, and quality. Biological significance Controlled deterioration treatment (CDT) is one of the major events that negatively affects the quality and nutrient composition of agricultural seeds. However, the molecular mechanism of CDT is largely unknown. A combination of gel-based and gel-free proteomic approach was utilized to investigate the effects of CDT in soybean seeds. Moreover, we utilized protamine sulfate precipitation method for enrichment of low-abundance proteins, which are generally masked due to the presence of high-abundance seed storage proteins. Reported data here serve as resource for its exploitation to metabolic engineering of seeds for multiple purposes, including increased seed viability, vigor, and quality.

Alydids and pentatomids are economic pests of leguminous crops and fruits in Korea. Direct impacts given by these phytophagous adult and nymphal by sucking on the pods, seeds and fruits can reduce germination potential of leguminous seeds, and yield poor nutritional quality. For monitoring of these bugs, various types of traps housing species-specific baiting with pheromones are widely practiced. However, a trap developed for one species has not been found to capture other species. Here, we developed a multi-species trapping device with some modification for enhancing capture efficacy of sympatric hemipteran bugs, called ‘rocket trap with solar fan’ pertaining to its shape. The trap with and without solar fan, and yellow, black, and white color stimuli were examined for the attractiveness of sympatric hemipteran bugs against conventional traps such as pyramid and fish-net traps. In addition, the attractiveness of single or mixed baits combination to sympatric hemipteran was also tested. Among the tested traps, yellow rocket trap with solar fan was found to attract significantly higher number of stink bug species. Major stink bugs, Riptortus pedestris , Halyomorpha halys , and Piezodorus hybneri were attracted to yellow rocket trap with solar fan baited with aggregation pheromone of H. halys in the fields. The yellow rocket trap baited with aggregation pheromones along with food and synergist was found more attractive to stink bugs. Results designate that yellow rocket trap with solar fan, and yellow rocket trap baited with mixed form of baits would have enhanced the monitoring and capturing sympatric hemipteran bugs in diversified agricultural landscapes.

The azuki bean weevil, Callosobruchus chinensis L., is one of the most serious stored product pests in the world. The temperature driven stage-specific development of C. chinensis was examined at eight constant temperatures of 15.97, 18.31, 22.09, 26.15, 30.50, 34.39, 38.00, and 40.00 °C on azuki bean ( Vigna angularis [Wild.] Ohwi & Ohashi) and cowpea ( Vigna unguiculata L.) seeds. Parameters for stage-specific development such as threshold temperatures and thermal constants were determined by linear and nonlinear models. The total developmental time (egg - adult) decreased with increasing temperature from 14 to 38 °C on both seeds. However, total developmental time varied from 127.93 to 122.25 days at 15.97 °C to 19.31 and 19.17 days at 38.00 °C on azuki bean and cowpea seeds, respectively. The nonlinear Logan-6 and Performance models estimated optimal temperatures as 37.3, 37.0, 36.5 and 37.1 °C on azuki bean, and as 36.7, 36.4, 34.5 and 36.6 °C on cowpea for egg, larva, pupa and egg to adult, respectively. The linear model estimated the lower temperature thresholds (T min ) of egg, larva, pupa and egg to adult as 16.97, 10.47, 1.36, and 10.28 °C on azuki bean, respectively, and 16.84, 10.33, 10.92, and 9.83 °C on cowpea, respectively. The thermal constants ( K ) for each stage completion was 35.13, 255.21, 233.11, and 503.41° days (DD) for egg, larva, pupa, and egg to adult on azuki bean, respectively and 32.45, 246.50, 107.04, and 490.02 DD for egg, larva, pupa, and egg to adult on cowpea, respectively. Temperature also influenced the adult longevity. These findings on thermal requirements and temperature thresholds can be used to predict the occurrence, number of generations and population dynamics of C. chinensis .

This study was conducted to obtain basic information on protein profile changes by artificial aging in soybean seeds. Seed proteins were extracted using the protamine sulfate precipitation method, which improves the detection of low-abundance proteins (LAPs) by depleting the major seed storage proteins . Isolated proteins were separated by high-resolution two-dimensional gel electrophoresis (2-DE), and differentially modulated protein spots were identified by MALDI-TOF/TOF. A total of 33 differential proteins were identified of which 31 and 2 showed decreased and increased abundances, respectively. Functional annotation of the identified proteins revealed that proteins were mainly associated with primary metabolism (55%) and response to stimulus (20.9%). Proteins with increased abundance were associated with nutrient reservoir activity (spots 5, 10), while the decreased abundance proteins were mainly involved in the primary metabolism such as carbohydrate metabolic process (spots 1–3, 11), protein folding (spots 6–9, 33), glucose metabolic process (spot 25) oxidoreductase activity (spots 19–24), UDP-glucose pyrophosphorylase activity (spots 12, 13). These results provide information about proteome changes, especially, LAPs during artificial seed aging treatment.

Uniformity and leveled distributions of soil chemicals across paddy fields are critical to manage optimal crop yields, reduce environmental risks and efficiently use water in rice cultivation. In this study, an investigation of spatial distributions on soil chemical properties was conducted to evaluate the effect of land leveling on mitigation of soil chemical property heterogeneity from a remodeled paddy field. The spatial variabilities of chemical properties were analyzed by geostatistical analyses; semivariograms and kriged simulations. The soil samples were taken from a 1 ha paddy field before and after land leveling with sufficient water. The study site was located at Bon-ri site of Dalseong and river sediments were dredged from Nakdong river basins. The sediments were buried into the paddy field after 50 cm of top soils at the paddy field were removed. The top soils were recovered after the sediments were piled up. In order to obtain the most accurate spatial field information, the soil samples were taken at every 5 m by 5 m grid point and total number of samples was 100 before and after land leveling with sufficient water. Soil pH increased from 6.59 to 6.85. Geostatistical analyses showed that chemical distributions had a high spatial dependence within a paddy field. The parameters of semivariogram analysis showed similar trends across the properties except pH comparing results from before and after land leveling. These properties had smaller “sill” values and greater “range” values after land leveling than ones from before land leveling. These results can be interpreted as land leveling induced more homogeneous distributions of soil chemical properties. The homogeneous distributions were confirmed by kriged simulations and distribution maps. As a conclusion, land leveling with sufficient water may induce better managements of fertilizer and water use in rice cultivation at disturbed paddy fields.

The interspecific estimation for tolerance capacities of upland crop species to excessive soil water stress in paddy field is significant in agricultural practices. Most of upland crops can be damaged by either excessive soil water or capillary rise of the water table during rainy season in paddy fields. The major objective of this study was to evaluate water stress of upland crops under different drainage classes in converted paddy field. This experiment was carried out in poorly drained soil (PDS) and imperfectly drained soil (IDS) of alluvial sloping area located at Toero-ri, Bubuk-myeon, Miryang-si, Gyeongsangnam-do. The soil was Gagog series, which was a member of the fine silty, mixed, nonacid, mesic family of Aeric Endoaquepts (Low Humic-Gley soils). Two drainage methods, namely under Open ditch drainage methods (ODM) and, Closed pipe drainage methods (PDM) were installed within 1-m position at the lower edge of the upper paddy fields. The results showed that sum of excess water days (SWD30), which was used to represent the moisture stress index, was 42 days (the lowest) in the PDM compared with 110 days in the ODM. Most of upland crops were more susceptible to excessive soil water during panicle initial stage on more PDS than on IDS. Yield of upland crops in the PDM was continuously increased by the rate of 15.1% on sorghum, 15.4% foxtail millet, 53.6% proso millet, 49.6% soybean and 47.9% adzuki bean as compared in the ODM. The capacity for tolerance by excessive soil water based on yield of each upland crop in the poorly drained sloping paddy fields was the order of sorghum, soybean, foxtail millet, proso millet and adzuki bean. Therefore, Sorghum is relatively tolerant to excessive soil water conditions and, may be grown successfully in converted paddy field.

The biodegradable film application can escape from plastic environmental pollution. This experiments studied the effect of biodegradable mulch film on the soil microbial community using fatty acid methyl ester method in soybean production field. The soil NO₃-N content in polyethylene mulch film (PE) soil was significantly higher than biodegradable mulch film soil (p ＜ 0.05). The soil microbial community of Gram negative bacteria showed significantly higher in biodegradable mulch film soil than PE mulch film soil (p ＜ 0.05). In addition, biodegradable mulch film soil had significantly low ratio of cy17:0 to 16:1ω7c and cy19:0 to 18:1ω7c compared with those of PE mulch film soil (p ＜ 0.05), indicating that microbial stress decreased. The ratio of cy17:0 to 16:1ω7c and cy19:0 to 18:1ω7c should be considered as a potential responsible factor for the obvious differentiation that was observed between the biodegradable mulch film soil and PE mulch film soil in a upland field. The results of this experimentation show the potential of using biodegradable mulch film in place of PE.

Chinese cabbage was cultivated in upland soil with the addition of biochar in order to investigate the potential for reduction of greenhouse gas emissions. Barley straw biochar (BSB) was introduced in a Wagner pot (1/5000a) in amounts of 0 (BSB0, control), 100 (BSB100), 300 (BSB300), and 500 (BSB500) kg 10a-1. After the addition of BSB into the upland soil, carbon dioxide (CO2) emission increased while methane (CH4) and nitrous oxide (N2O) emissions decreased. The highest CO2 flux was measured for the BSB500 sample, (84.6 g m-2) followed by BSB300, BSB100, and BSB0 in decreasing order. Relative to those of control, the total CH4 flux and N2O flux for the BSB500 treatment were lower by 31.6% and 26.1%, respectively. The global warming potential (GWP) of the treatment without biochar was 281.4 g CO2 m-2 and those for treatments with biochar were in the range from 194.1 to 224.9 g CO2 m-2. Therefore, introducing BSB into upland soil to cultivate Chinese cabbages can reduce the global warming potential.

Direct seeding of sorghum (Sorghum bicolor L. Moench) has a problem of low yield including poor establishment. This poor establishment results from poor quality seed, poor seedbed preparation, seedling pests, poor sowing technique and high soil temperature. This study sought to establish the age at which sorghum seedlings can be transplanted with minimal effects on grain yield. Transplants were raised in 128 nursery tray pot. Five seedling ages were established by transplanting at 10 (T10), 15 (T15), 20 (T20), 25 (T25) and 30 (T30) days after planting (DAP). The treatment combinations were arranged in a randomized complete block design and replicated three times with an individual plot size of . Each plot had five ridges with a planting space of at one plants per stand. Results showed that seedling age on transplanting significantly affected growths and yields to sorghum after transplanting. Plant heights and diameters of transplants at T15 were longer than the other transplants. Conclusively, The advantages of this practice were better control of crop density and greater yields; either to fill gaps after emerging and thinning of crops or to compensate for a growth period that was too short for a complete crop cycle.

Soybean mosaic virus (SMV) is a prevalent pathogen that causes significant yield reduction in soybean production worldwide. SMV belongs to potyvirus and causes typical symptoms such as mild mosaic, mosaic and necrosis. SMV is seed-borne and also transmitted by aphid. Eleven SMV strains, G1 to G7, G5H, G6H, G7H, and G7a were reported in soybean varieties in Korea. A reverse transcription loop-mediated isothermal amplification (RT-LAMP) method allowed one-step detection of gene amplification by simple procedure and needed only a simple incubator for isothermal template. This RT-LAMP method allowed direct detection of RNA from virus-infected plants without thermal cycling and gel electrophoresis. In this study, we designed RT-LAMP primers named SML-F3/B3/FIP/BIP from coat protein gene sequence of SMV. After the reaction of RT-LAMP, products were identified by electrophoresis and with the detective fluorescent dye, SYBR Green I under daylight and UV light. Optimal reaction condition was at 58°C for 60 min and the primers of RT-LAMP showed the specificity for nine SMV strains tested in this study.

In order to develop optimum paddy-upland rotation system, we evaluated the 1st and the 2nd upland growth and yield characteristics of foxtail millet, proso millet, sorghum rotated from paddy field and rice rotated from upland in paddy-upland rotation. Average number of ears per hill was 3.3 in the 2nd upland cultivation. The value was greater by 1 ear as compared to 1st upland cultivation (2.2 ears per hill). In average yield per 10a, the 2nd upland cultivation showed 220.3 kg, 23% increased yield compared to the 1st upland cultivation (179 kg per 10a). In average number of ears per hill, the 2nd upland cultivation showed 8.3 ears, increased 4 ears compared to the 1st upland cultivation (4.2 ears per hill). In average yield per 10a, the 2nd upland cultivation showed 152.8 kg, 16.8% increased yield compared to the 1st upland cultivation (130.8 kg per 10a). In average days from seeding to heading of 5 sorghum varieties, there were no significant difference between the 1st (68.6 days) and the 2nd (67.4 days) upland cultivation rotated from paddy field. In the average number of grains per ears, the 2nd upland cultivation showed 2,931.6 grains per ear, 12% increased compared to the 1st upland cultivation (2,619.6 grains per ears). Average yield per 10a of sorghum in the 2nd upland cultivation showed 242.3 kg, 4.6% increased compared to the 1st upland cultivation (231.7 kg per 10a). In growth and yield characteristics of rice in paddy-upland rotation, culm length in paddy-upland-paddy plot showed 82.9 cm, 7.3 cm longer compared to the continuous rice paddy field (75.6 cm). Ear length was also 1 cm longer than that of the continuous rice paddy field. In average number of ears per hill, paddyupland- paddy plot showed 25.0 ears, 4.3 ears more than that of the continuous rice paddy field (20.7 ears per hill). In average yield of rice per 10a, the paddy-upland-paddy rotation plot showed 526.8 kg, 9.8% higher yield compared to the continuous rice paddy field (479.9 kg per 10a).

Soybean is one of the important food crop around the world. Especially in East Asia, it is the main ingredient for traditional food like soy sauce and soy paste. The double cropping system including soybean following onion, Chinese cabbage, and potato is widely adopted in Southern region of Korea. In this system, sowing date of second crop (soybean) can be delayed depending on first crops' growth period and weather condition. When planting date is delayed it is known that soybean yield is declined because of shorter vegetative growth period and earlier flowering induced by warm temperature and changes in photoperiod. The objective of this study was to determine soybean growth and yield responses as plant populations at late planting date. Field experiment was conducted at Department of Functional Crop, National Institute of Crop Science, RDA located in Miryang, Gyeongsangnam-Do for two years ('13-'14) in upland field with mid-late maturity cultivar Daewon. A split-plot block design was used with three replications. Main plots were three sowing dates from June 20 to July 20 with 15 days intervals, and subplots were 4 levels of planting densities. Data of maturity (R8) was recorded, yield components and yield were examined after harvesting. Experimental data were analyzed by using PROC GLM, and DMRT were used for mean comparison. Optimum planting population for maximizing soybean yield in late planting which compared with standard population. In mid-June planting, higher planting density causes increased plant height and decreased diameter which lead to higher risk of lodging, however, reduced growth period due to late planting alleviated this problem. Therefore higher seeding rates can provide protection against low seedling emergence caused by late planting in this region.

Rice stripe virus (RSV) is a major rice-infecting virus in Korea that has caused a severe resurgence in the western provinces in 2007–2008. The outbreak of RSV in rice was caused by the small brown planthopper Laodelphax striatellus (an RSV vector), which overwinters in temperate climates. Mass immigration of its insect vector from China has been proposed as a critical risk factor in development of the disease. A nationwide survey was done in June and July 2012–2013 in 30 Korean provinces to confirm RSV sequence variation. The sequence of the nucleocapsid protein (NCP) gene and the intergenic region (IR) of RSV RNA3 was analyzed. A phylogenetic analysis of the NCP gene and the IR demonstrated that the 33 isolates were closely related to earlier reported isolates from Japan and China, but the secondary IR structure showed strong geographic clustering. This is the first report on a nationwide RSV survey of Korea. The results will provide information on the relationships among RSV isolates to understand the epidemiology of this rice disease and the effect of climate changes and insect migration.

The effects of three temperatures, 20 °C, 25 °C and 30 °C, and four relative humidity (RH) levels, 30–35%, 50–55%, 70–75%, and 90–95%, on oviposition and development of Callosobruchus chinensis (L.) (Coleoptera: Bruchidae) were investigated. C . chinensis were introduced to 30 g azuki bean seeds (cultivar Hongeon) one day post emergence. Five pairs of male and female beetles were used for each combination of temperature and RH, and replicated ten times. The introduced adults were allowed to lay eggs for 72 h. Temperature significantly influenced oviposition with increased number of eggs at 30 °C. Relative humidity had no effect on egg laying. Development time was affected by both temperature and RH. Development time was shortest at 30 °C and 70–75% RH. Adult emergence rate was affected by both temperature and RH; adult emergence rate increased with the increase in temperature and RH (up to 70–75%) levels. The results suggest that rather than the interactive effect of temperature and RH, individual effects of temperature and RH on C . chinensis are profound and C . chinensis is likely to cause greater damage to azuki bean seeds stored at 30 °C and 70–75% RH.

This study was conducted to serve as a basis for establishing a stable production of proso millet on the rotational paddy-upland field by looking out the physicochemical and moisture characteristics of soil and the growth characteristics of proso millet by ridge width. Plant height showed Manhongchal was the smallest 71.3 cm in 240 cm wide of ridge in the first year, Ibeakchal was the highest 69.7 cm in 60 cm wide in the second year, Hwanggeumchal was the highest 72.8 cm in 60 cm wide in the first year. The retention time of the excess water in the soil during cultivation was extended wider the width of ridge. The variation width of the water content was higher by the wider the width of ridge. Yield components showed the longest ear length were 35.1 cm of Manhongchal, 34.8 cm of Ibeakchal in 60 cm wide of ridge in the first year. As the width of ridge extended, ear weight of all variety increased. 1,000 seed weight of the 60 cm wide of ridge was Manhongchal 6.8%, Ibeakchal 46.2% heavier than the 240 cm wide of ridge. Yield showed Manhongchal 221, Ibeakchal 223, Hwanggeumchal in 60 cm wide of ridge in the first year. The similar pattern of amount showed Manhongchal 278, Ibeakchal 221, Hwanggeumchal in 60 cm wide of ridge in the second year. This showed Manhongchal 103%, Ibeakchal 119%, Hwanggeumchal 85.2% was more than the 240 cm wide of ridge.

우리나라 중부지역에서 파종기이동과 멀칭유무에 따른 조, 기장, 수수의 최적파종기 및 파종한계기 구명을 위하여 5월 15일, 6월 6일, 6월 26일, 7월 13일 파종기를 4수준으로 하여 피복 및 무피복을 처리하였다. 시험품종은 조 4품종(삼다찰, 삼다메, 경관1호, 황금조), 기장 4품종(이백찰, 만홍찰, 황실찰, 황금기장), 수수 4품종(남풍찰, 황금찰, DS202, 목탁수수)을 국립식량과학원 기능성작물부와 농업기술실용 화재단에서 분양받아 2013년 경기도 안성시에서 실시한 시험결과를 요약하면 다음과 같다. 1. 간장은 조, 기장, 수수 모두 6월 6일 파종기에서 가장 컸다. 2. 수장은 조, 기장은 5월 15일 파종기에서 가장 컸고 수수는 6월 26일 파종기에서 가장 컸다. 3. 엽수는 조, 기장, 수수 모두 5월 15일 파종기에서 가장 많았다. 4. 조의 출수소요일은 파종기가 늦어짐에 따라 삼다메와 경관1호, 삼다찰은 감소하였고 황금조는 1차 파종기인 5월 15일부터 3차 파종기인 6월 26일 파종기까지는 감소하다가 7월 13일 4차 파종기에서는 6월 26일 파종기와 같았다. 기장의 출수소요일은 4품종 모두 파종기가 늦어짐에 따라 감소하였다. 수수의 출수소요일도 4품종 모두 파종기가 늦어짐에 따라 감소하다가 마지막 파종기인 7월 13일에는 증가하였다. 5. 멀칭 및 무멀칭구 간에는 조, 기장, 수수 3작물 모두 생육 및 수량 특성간 유의적인 차이가 없었다. 6. 1수립수, 천립중 및 10당 수량은 조, 기장, 수수 모두 6월 26일 파종기에서 가장 높았다. 중부지방에서 조, 기장, 수수의 최적 파종기는 6월 하순으로 판단된다. 7. 7월 13일 파종기에서는 황금조, 만홍찰, 황금기장을 제외한 나머지 품종들은 만파로 인하여 성숙기에 이르지 못하였다. 중부지방에서 조, 기장, 수수의 파종 한 계기는 7월 초순으로 판단된다. 【In order to investigate optimum and marginal sowing date of foxtail millet, proso millet and sorghum in middle area (Anseong, Gyeonggi province) of korea, four levels of sowing date [(May 15(1st), June 6(2th), June 26(3th), July 13(4th)] with mulching and non-mulching were treated in this experiment. We used three crops of foxtail millet, proso millet and sorghum with four varieties of Samdachal, Samdamae, Kyeongkwan1, Hwanggeumjo in foxtail millet, Leebaekchal, Manhongchal, Hwangsilchal, Hwanggeumgijang in proso millet and Nampungchal, Hwanggeumchal, DS202, Moktaksusu in sorghum achieved from RDA. In culm length, sowing date of June 26 was the highest in all varieties of foxtail millet, proso millet and sorghum. Sowing date of May 15 showed the highest ear length in foxtail and proso millet, whereas the highest ear length was obtained from sowing date of June 26 in sorghum. In numbers of leaf, sowing date of May 15 showed the highest in all three crops of foxtail millet, proso millet and sorghum. Days from sowing to heading date were reduced in Samdamae, Kyeongkwan1 and Samdachal as sowing date was late. In Hwanggeumjo it was reduced from the sowing date from May 15 to June 26, but it was same days was taken between June 26 and July 13 sowing date. Days from sowing to heading date of four varieties in proso millet were reduced as sowing date was late. Days from sowing to heading date in sorghum were reduced as sowing date was late, whereas it was increased the $4^{th}$ sowing date of July 13. There were no significant differences in growth and yield characteristics between mulching and non-mulching in four varieties of foxtail millet, proso millet and sorghum with four varieties. The highest grain numbers per panicle, 1000 grain weight and yield per 10a were obtained from sowing date of June 26 in four varieties of foxtail millet, proso millet and sorghum. In sowing date of July 13, all varieties of foxtail millet, proso millet and sorghum were not able to be ripened because of late sowing except Hwnaggeumjo, Manhongchal and Hwanggeumgijang.】

농경지의 볏짚 수거량이 증가하면서 부족한 유기물을 공급하기 위하여 녹비작물을 이용하고 있다. 녹비작물은 유기물공급과 화학비료 대체가 가능한 우수한 유기물원이다. 그러나 농경지에서 분해되는 과정에 메탄을 발생시키기 때문에 메탄발생량을 줄이기 위한 노력이 요구된다. 따라서 논토양에서 녹비작물을 이용할 때 메탄발생량을 줄이기 위하여 경운깊이를 달리하여 시험하였다. 벼 생육기간 중 메탄 발생량은 이앙 후 63일, 74일에 가장 많았고 이앙 후 74일 이후부터 감소되었으며 이앙 후 106일에는 거의 발생되지 않았다. 녹비종류에 따른 메탄발생량은 보리환원구에서 가장 많았고, 그 다음은 헤어리베치, 화학비료 순이었다. 경운 깊이에 따른 메탄발생량은 10cm경운보다 20 cm로 경운함으로써 화학비료는 22.5%, 헤어리베치 환원구는 12.4%, 보리환원구는 11.7% 감소되었다. 벼 재배기간 동안 대기온도는 $30{\sim}40^{\circ}C$ 였고, 지온은 대기온도보다 약 $2{\sim}10^{\circ}C$ 정도 낮았다. 산화환원전위(Eh)는 이앙 전 관개를 시작하면서 급격히 토양이 환원되어 (-)값을 나타냈다. 작물 재배기간 동안 산화환원전위차는 -300~-500 mV으로 낮았으며 관개가 중단된 이앙 후 106일 이후에는 다시 급격하게 증가되었다. 쌀 수량은 경운깊이에 따른 차이는 없었으나 녹비작물중에서는 헤어리베치 환원구에서 가장 많았다. 논토양에서 헤어리베치를 이용할 때 20cm로 경운하여 메탄발생을 효과적으로 줄일 수 있었다. 【Green manure crops are organic materials that can supply organic matter and substitute chemical fertilizer, yet emit methane while being decomposed. Therefore, we experimented with different kinds of Green manure crops and tillage depth in order to decrease the amount of methane emitted when utilizing Green manure crops in paddy soil. The amount of methane emitted during the cultivation period of rice started to increase after transplanting and peaked at 63, and 74 days after transplanting, than decreased to almost none starting from 106 days. According to the kind of Green manure crop, it was highest in barley, then hairy vetch and chemical fertilizer. Depending on the tillage depth, the amount of methane emitted decreased by 22.5% in chemical fertilizer, 12.4% in hairy vetch and 11.7% in barley in 20cm tillage compared to 10cm tillage. The air temperature of methane test period was $30{\sim}40^{\circ}C$ , and the soil temperature was more than about $2{\sim}10^{\circ}C$ lower than the air temperature. Due to the irrigation started before transplanting, the oxidation-reduction potential (Eh) of soil was rapidly reduced, and showed negative (-) values. Eh values mostly kept the range of -300~-500 mV during rice cultivation. It rapidly increased 106 days after transplanting. Rice yield the highest in hairy vetch and did not show differences according to tillage depth. Methane emission could be effectively reduced if the paddy soil was tilled by 20 cm during the application of hairy vetch.】

A sequential planting method was developed to screen rice plants with durable resistance against rice blast in a short time, and applied for several years in Korean rice breeding program. In this study, we showed the advantages of a sequential planting method compared to other pathogenicity tests. The correlation analysis among three pathogenicity tests and other factors demonstrated that durable resistance depended on the average of diseased leaf area and the number of compatible pathogens. Significant correlations were found in the nursery test but not in the field test result. In addition, we traced changes in the pathogen population during sequential planting stages through re-isolation of the pathogen. The portion of compatible pathogens was increased during sequential planting. Through this study, we provide an effective sequential planting method and direction of durable resistance in a breeding program.

Stable production of green manure crop is one of the critical factor for successful implementation of environmental-friendly agriculture in South Korea. Most green manure crops including hairy vetch cultivated in South Korea must be passed cold winter season. This study was conducted to establishment of cultivation zones on green manure crops under paddy field in South Korea using on minimum temperature and drainage class. In order to build the spatial distribution map, hairy vetch, triticale, barley, rye, and clover as green manure crop were selected and mean of daily minimum temperature and drainage class in paddy field were considered as limiting factor for stable growth of green manure crop. And cultivation zone on green manure crops was divided into two categories; `Availability area` and `Safety area`. Low limit temperature on green manure crops in this study was the following. Temperature on `Availability area` was , , , and for hairy vetch (including triticale), barley, clover, and rye respectively. And temperature on `Safety area` was lower compared to that of `Availability area` exception rye which was the same degree. Rye was expected to safely cultivated the almost whole paddy field (99.71%) of South Korea. `Safety area` among the whole paddy field for hairy vetch, barley, and clover were 86.58, 53.06, and 15.76% respectively. The spatial distribution map of green manure crops reestablished in this study could be used the policy and agricultural management for environmental-friendly agriculture using green manure crops in South Korea.

Serious problems in reclaimed land agriculture are high soil salinity and poor vertical drainage, so desalinization in these soils is very difficult. Also, although desalinization is accomplished in reclaimed top soils, before long, soils are resalinized according to capillary rise of salts from the subsurface soils. To resolve these problems, multi-layered soil columns with subsurface layer of macroporous medium utilizing coal bottom ash (CBA) were constructed and the effects of blocked resalinization of these soils were investigated. In this experiment soil samples were collected from Munpo series (coarse-loamy, nonacid, mixed, mesic, typic Fluvaquents). The soil texture was silt loam and the EC was 33.9 dS m -1 . As for groundwater seawater was used and groundwater level of 1 cm from the bottom was maintained. The overall rate of capillary rise was 2.38 cm hr -1 in soil 60 cm column, 0.25 cm hr -1 in topsoil (30 cm) + CBA (5 cm) + subsurface soil (10 cm) column and 0.08 cm hr -1 in topsoil (30 cm) + CBA (10 cm) + subsurface soil (10 cm) column. In multi-layered soil columns with CBA 20, 30 cm layer, wetting front due to capillary rise could not be seen in top soil layer. After 70 days capillary rise experiment water soluble Na+ accumulated in top soil of soil columns with CBA 20, 30 cm was diminished by 92.8, 96.5% respectively in comparison with Na+ accumulated in top soil of soil 60 cm column because CBA layer cut off capillary rise of salts from the subsurface soil. From these results we could conclude that the macroporous layer utilizing CBA placed at subsurface layer cut off capillary rise of solutes from subsurface soil, resulting in lowered level of salinity in top soil and this method can be more effective in newly reclaimed saline soil.

For application of sodium hypochlorite as a seed disinfectant to the control of bakanae disease caused by Gibberella fujikuroi in rice, we investigated the effects of sodium hypochlorite for antifungal activity, eliminating fungus from seeds and reducing disease occurrence in vitro and greenhouse. The viability of the pathogen was significantly reduced at 80 ml/l concentration of sodium hypochlorite, and the pathogens did not grow at over 100 ml/l concentration of sodium hypochlorite. The effect of eliminating fungus was 90% at treatment of 0.3% sodium hypochlorite solution to infected rice seeds for eight hours. When the rice seeds were soaked into 0.5% and 0.3% sodium hypochlorite solutions for twelve hours, the disease incidences of rice seedling were remarkably reduced to 4.3% and 4.7%, respectively, compared to 97.3% of non-treatment control. The rates of seedling stand were 29.1% and 26.9% higher with the sodium hypochlorite treatment than that of non-treatment control. When prochloraz and sodium hypochlorite was treated to naturally severely infested rice seeds with bakanae disease, the disinfection effect was higher than that of prochloraz alone treatment. When the seeds were soaked in sodium hypochlorite before or after prochloraz, the rate of seed contamination was low as 4.0% or 6.3%, respectively, compared to prochloraz alone as 13.7%. The disease incidence was low as 3.7% or 8.3%, respectively, compared to prochloraz alone as 14.3%. The disinfection effect of treatment with prochloraz after sodium hypochlorite was higher than that of treatment with prochloraz before sodium hypochlorite.

Landscape crops have decorated its surrounding landscape by being cultivated from spring to late fall. Recently, landscape crops are starting to get the limelight as crops that can be cultivated in large area farmlands. Therefore, we examined the growth characteristics of landscape crops, green manure yield and nitrogen production of crops that are cultivated during summer, which are sunflower, cosmos, sunnhemp and sesbania, in order to select crops that can be utilized as landscape crops and green manure crops. The height of landscape crops increased when the harvest time was later. Especially, sunnhemp, cosmos, sunflower and sorghum grew over 100cm. The days to flowering of sunnhemp and cosmos were 50 and 53 days each, and their flowering period of more than 50 days were long. The days to flowering of sunflower, which was 52 days, was short, and its flowering period, which was 21 days, was also short. When the harvest time was later, the green manure yield and nitrogen production of all crops increased. Individually, the green manure yield was higher in sunnhemp, sorghum, sunflower, with 7.2~7.5 Mg ha -1 , and was lowest in sesbania. The nitrogen production was higher in sunnhemp, with 168.1 kg ha -1 Therefore, as seen in its flowering characteristics, green manure yield and nitrogen production, sunnhemp had the best green manure and landscape effects among the landscape crops for summer.

This paper provides some of the scientific background on how projected environmental conditions could affect weeds and weed management in crops. Elevated CO₂ levels may have positive effects on crop competitiveness with C₄ weeds, but these are generally outnumbered by C₃ species in weed populations. Moreover, higher temperatures and drought will favor C₄ over C₃ plants. The implementation of climate change adaptation technologies, such as drought-tolerant germplasm and water-saving irrigation regimes, will have consequences for crop?weed competition. Rainfed production systems are thought to be most vulnerable to the direct effects of climate change and are likely to face increased competition from C₄ and parasitic weeds. Biotic stress-tolerant crop cultivars to be developed for these systems should encompass weed competitiveness and parasitic-weed resistance. In irrigated systems, indirect effects will be more important and weed management strategies should be diversified to lessen dependency on herbicides and mechanical control, and be targeted to perennial rhizomatous (C₃) weeds. Water-saving production methods that replace a weed-suppressive floodwater layer by intermittent or continuous periods of aerobic conditions necessitate additional weed management strategies to address the inherent increases in weed competition. Thus, climatic conditions have a great effect on weed population dynamics all over the world.

Several tomato production regions in Korea were surveyed for tomato yellow leaf curl disease (TYLCD). Tomato leaf samples showing TYLCD-like symptoms were collected from Tongyeong (To), Geoje (Gi), and Gimhae (Gh) cities of the southern part of Korea. Tomato yellow leaf curl virus (TYLCV) was detected and the full-length genomes of the isolates were sequenced. The TYLCV isolates found in Korea shared high sequence identity (> 99%) with TYLCV-IL [JR:Omu:Ng] (AB110217). Phylogenetic relationship analysis revealed that they formed two groups (with little genetic variability), and the To, Gj, and Gh isolates belonged to the TYLCV-IL group. An infectious clone of TYLCV-To (JQ013089) was constructed and agroinoculated into Nicotiana benthamiana, Nicotiana tabacum var. Xanthi, Petunia hybrida, Capsicum annuum, and Lycopersicon esculentum cv. Hausumomotaro. Agroinfection with a dimeric infectious clone of TYLCV-To induced severe leaf curling and stunting symptoms in these plants, excluding C. annuum. Tomato plants then developed typical yellow leaf curl symptoms.

In order to evaluate water balance at upland according to RCP8.5 climate change scenario distributed by Korean Meteorological Administration (KMA), we simulated soil moisture using estimation model, called AFKAE0.5 for 66 sites from 2011 to 2020, and established the water balance maps. The amount of annual average precipitation by RCP8.5 scenario was highest in 2016 as recorded 2,062 mm and lowest in 2011 with 1,134 mm. As result of analysis for monthly precipitation and runoff, the amounts of precipitation and runoff have been especially intensive in July in 2014, 2016, 2019, and 2020. Overall, the area of Kyeongbuk and Gyeonggi was estimated more dried status of soil compared with precipitation. Except 2015 and 2020, soil water balance was recorded as negative value in other years which was calculated by subtracting output from input. The status of soil moisture was the most dry in 2020 among those in other years.

In order to evaluating physiochemical properties of soil under minimum tillage and direct seeding cultivation on dry rice paddy, we conducted to analyze the soil physiochemical characteristics in treatment with 2-year minimum tillage and dry direct seeding (2MT), 3-year minimum tillage and dry direct seeding (3MT), and tillage transplanting cultivation (TT). As results of analyzing soil organic matter (OM) contents with 2 cm soil depth of interval from surface to 30 cm, OM contents with surface soil from 0 to 2MT and 3MT were higher than TT, recorded 34.6, 28.1 and 19.8 g kg -1 , respectively. But until 20cm in soil depth, it was not so large on the deviation of OM contents among the 3 treatments comparing with 2cm surface. Beneath 20 cm in soil depths, 2- and 3-year, OM contents in TT were distributed to be lower than 2MT and 3MT. The contents of total nitrogen in 2MT and 3MT were higher than the content in TT across the soil profile. Consequently, though minimum tillage and direct seeding farming is obviously the practice to saving of machinery work and labor, other practices such as continuously input OM should be needed to achieve carbon sequestration goal through minimum tillage and direct seeding on dry paddy.

Effect of Incorporation Times of Green Barley and Hairy Vetch on Rice Yield in Paddy Soil with Liquid Pig Manure Se-Won Kang, Dong-Cheol Seo, Sang-Gyu Lee, Young-Jin Seo, Ju-Wang Park, Jin-Hee Ryu, Min-Tae Kim, Hang-Won Kang, Jong-Soo Heo and Ju-Sik Cho (Department of Bio-Environmental Sciences, Sunchon National University, National Institute of Crop Science, Rural Development Administration, Division of Applied Life Science(BK21 program) & Institute of Agriculture and Life Science, Gyeongsang National University)

To investigate the effect of partial tillage seeding of hairy vetch on green manure biomass and rice yield, on-farm experiment was conducted at eco-friendly hairy vetch cultivation area located in Yesan-gun, Chungcheongnam-do. Seeding methods of hairy vetch consisted of partial tillage seeding (PTS) and broadcasting before rice harvesting (BBRH). Hairy vetch was incorporated into soil on May 18 and rice seedling was transplanted on May 26. The growths of hairy vetch before overwintering were investigated on November 11. Plant height of BBRH plot was longer than that of PTS plot, but somewhat larger number of seedling stand was found in PTS. Biomass and N production of hairy vetch were investigated on May 18. Results of the investigation showed no difference between two seeding methods. Although the seeding time was 21 days late in PTS, the biomass of hairy vetch and rice yield were equivalent to those of BBRH seeding, so we conclude that PTS could improve overwintering survival of hairy vetch in rice cropping system.

Massive intercalation of urea into montmorillonite (MUCH) was recently proposed to enhance urea use efficiency through smart suppression of emission of NH3 and NOx. This study was to synthesize citrate-incorporated MUCH (Cit-MUCH) which can enhance suppression of NH₃ volatilization. The XRD pattern of Cit-MUCH was very similar to that of MUCH to indicate successful incorporation of citric acid into MUCH. Incorporation of citric acid was confirmed by the existence of COO- symmetric stretching vibration. During the initial 4 days after application, NH₃ volatilization from both bare and perilla-planted soils was much more suppressed by application of Cit-MUCH than MUCH. A peak volatilization rate decreased from 28.3 N mg m -2 h -1 of MUCH-broadcasted soil to 22.2 N mg m -2 h -1 of Cit-MUCH-broadcast soil. NH₃ volatilization was less in planted soil than bare soil for 72 hrs after application. These results showed that incorporation of citric acid led to increase in suppression of ammonia volatilization from urea-applied soils.

In order to evaluate drought risk at upland according to climate change scenario (RCP8.5), we have carried out the simulation using agricultural water balance estimation model, called AFKAE0.5, at 66 weather station sites in 2020, 2046, 2050, 2084, and 2090. Total Drought Risk Index between the first month (f) and last month (l) (TDRI(f/l)) and maximum continuous drought risk index (MCDRI(f/l)) were defined as the index for analyzing pattern and strength of drought simulated by the model. Based on distribution maps of MCDRI (1/12), drought strength was predicted to be most severe in 2084 for all regions. Some regions showed severe risk of drought meaning over 20 days of MCDRI (1/12) in the other years, while MCDRI (1/12) in other regions did not reach 5 days. Even though maximum value of TDRI (1/12) in 2090 was greater than in 2050, more severe drought risk in 2050 than in 2090 was predicted based on MCDRI (4/6). It implies that drought risk should be assessed for each crop with its own growing season.

The effects of germanium dioxide (GeO2) and 2-carboxyethyl germanium sesquioxide (Ge-132) treatments on cucumber (Cucumis sativus L.) growth and Ge accumulation and toxicity were investigated. Accumulated Ge contents in plant treated with GeO2 were two times greater than that in plants treated with Ge-132. Germanium accumulated primarily in the shoots in the GeO2 treatments and in the roots in the Ge-132 treatments. In contrast GeO2 was easily transported from roots to shoots. In cucumber fruit, the range of Ge content in both the GeO2 and Ge-132 treatments was in the order of stalk > rind > pulp > seeds, suggesting that Ge accumulation was based on distance from xylem. Silicon (Si) content significantly decreased as GeO2 concentration increased, but there was no difference in Si content in the Ge-132 treatment. Therefore, plant growth and Ge accumulation are affected differently by GeO2 and Ge-132 treatments, based partly on treatment concentrations.

This study was conducted to select alternative herbicides to control herbicide-resistant and - troublesome weeds in rice fields, Korea. The resistant Echinochloa oryzicola to ACCase inhibitor was tested by herbicides registered to control Echinochloa oryzicola. And the resistant and troublesome weeds to sulfonylurea (SU) herbicides, ALS inhibitors, were tested by widely using herbicides to control the resistant weeds in Korea. The oxadiazon, pyrazolate, pretilachlor and benzobicyclone+thiobencarb effectively controlled resistant Echinochloa oryzicola to ACCase and ALS inhibitors at 0.5 leaf stage of the, Herbicides containing mefenacet or fentrazamide controlled effectively by the 2 leaf stage. In controlling other SU resistant weeds including Sagittaria trifolia and Ludwigia prostrate, benzobicyclon and mesotrione performed well all tested weeds, and carfentrazone and pytazolate were effective to broadleaf weeds. Sagittaria trifolia and Ludwigia prostrate, which were suspected to be resistance to SU herbicides, were well controlled by carfentrazone and pytazolate.

This study was tried to control the weedy rice using minimum tillage direct seeding of rice on dry paddy. Minimum tillage direct seeding on dry paddy is tilled with 4 cm in width at 27 cm intervals by 1-shaped rotary blade. Using this cultivation method can control weedy rice effectively by minimizing the soil disturbance. In this study, weedy rice was firstly emerged at April 28 in 2012. After 11 days emergence rate of weedy rice at May 9 was 61.9%. At this time rice was sown and then herbicides was treated at 10 and 15 days after sowing. When herbicides was treated at 10 days after sowing the control effect was 89-96%, and when treated at 15 days the control effect was 97-99%. However emergence of weedy rice differ according to the year and year. It was thought to be affected by April average temperature and total precipitation. So, for effective weedy rice control and stable rice yield, it was considered that irrigation at April 15-20 to induce the emergence of weedy rice is required.

Hairy vetch can fix nitrogen from the atmosphere as a leguminous cover crop. This research was carried out to determine optimum seeding method of hairy vetch and application effect in paddy. Field experiment was conducted at Sinheung series (fine loamy, mixed, nonacid, mesic family of Fluvaquentic Endoaquepts) from Sep. 2011 to Oct. 2012 at the National Institute of Crop Science (NICS), RDA, Suwon, Gyeonggi province, Korea. Hairy vetch used in the study was ‘Cheongpyungbora’, developed by National Institute of Crop Science. Seeding methods of hairy vetch consisted of Broadcasting Before Rice Harvesting (BBRH), Partial Tillage Seeding (PTS), Minimum Tillage Seeding (MTS), No Tillage Seeding (NTS), and Drill Seeding (DS). Both MTS and NTS showed the highest biomass among the seeding methods. The rice yield of MTS and NTS significantly increased compared to conventional fertilization (CF). Also soil properties including organic matter and bulk density were improved by incorporation of hairy vetch. Therefore, we suggested that MTS and NTS could be used to produce hairy vetch and rice in paddy.