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382 results on '"Hanano, Manabu"'

11. Kinetics of active efflux via choroid plexus of beta-lactam antibiotics from the CSF into the circulation

Author

Ogawa, Makoto, Suzuki, Hiroshi, Sawada, Yasufumi, Hanano, Manabu, and Sugiyama, Yuichi

Subjects
Choroid plexus -- Research, Probenecid -- Analysis, Biological sciences
Abstract

Studies on the role of choroid plexus in clearing toxic anions from cerebrospinal fluid confirm the role of choroid plexus in the process and indicate the usefulness of isolated choroid plexus in the in vivo elimination of organic anions. Organic anions, including probenecid, ampicillin, cefodizime, cefotaxime and ceftriaxone, reduce the transport activity in a dose-dependent manner.

Published
1994

13. Different relationships between cellular ATP and hepatic uptake among taurocholate, cholate and organic ions

Author

Yamazaki, Masayo, Suzuki, Hiroshi, Hanano, Manabu, and Sugiyama, Yuichi

Subjects
Adenosine triphosphate -- Physiological aspects, Sodium metabolism -- Physiological aspects, Anions -- Physiological aspects, Liver -- Physiological aspects, Biological sciences
Abstract

The effects of varying cellular ATP conditions on the uptake of bile acids and organic anions were studied in isolated rat liver cells. The mechanism of sodium-dependent cholate uptake was found to be distict from that of taurocholate. The sodium-independent uptake of bile acids and organic anions suggest the involvement of ATP hydrolysis or a secondary active transport with an ion gradient that diffuses faster than the sodium gradient.

Published
1993

14. Na+ -independent multispecific anion transporter mediates active transport of pravastatin into rat liver

Author

Yamazaki, Masayo, Suzuki, Hiroshi, Hanano, Manabu, Tokui, Taro, Komai, Toru, and Sugiyama, Yuichi

Subjects
Pravastatin -- Physiological aspects, Biological transport -- Research, Liver cells -- Physiological aspects, Cholesterol metabolism -- Research, Biological sciences
Abstract

The selective inhibition of hepatic cholesterol synthesis by pravastatin, an inhibitor of the hydrophilic 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase, was investigated. Pravastatin uptake by isolated rat hepatocytes was evaluated to test the possibility of the existence of a specific uptake mechanism for pravastatin in the liver. The results showed that a Na+ -independent, carrier-mediated active transport mechanism is involved in pravastatin uptake. This could account for the in vivo selective inhibition of hepatic cholesterol synthesis by pravastatin.

Published
1993

35. Measurement of cerebral glucose utilization from brain uptake of [14C]2-deoxyglucose and [3H]3-O-methylglucose in the mouse

Author

Hanano Manabu, Sugiyama Yuichi, Sawada Yasufumi, Iga Tatsuji, Suzuki Hiroshi, Ishizuka Hitoshi, and ito Kiyomi

Subjects
Male, Pentobarbital, medicine.medical_specialty, Mice, Inbred Strains, 3-O-Methylglucose, Deoxyglucose, Models, Biological, Urethane, Body Temperature, Dephosphorylation, Mice, In vivo, Internal medicine, Deoxy Sugars, medicine, Animals, Anesthesia, Phosphorylation, Distribution Volume, Pharmacology, Volume of distribution, Methylglycosides, Chemistry, Brain, Methylglucosides, Metabolism, Carbon Dioxide, Hydrogen-Ion Concentration, Oxygen, Glucose, Endocrinology, Anesthetic, medicine.drug
Abstract

Glucose utilization (GU) in the mouse brain in vivo was measured by the simultaneous use of [ 14 C]2-deoxyglucose (2DG), the glucose analogue that can be phosphorylated in the brain, and [ 3 H]3-O-methylglucose (3MG), the nonmetabolizable glucose analogue. Originally, this method was developed by Gjedde et al. (1985) in the rat and in humans. The present study examined the validity of this method in the mouse brain. The effects of urethane and pentobarbital (PB) on GU were also studied. Whereas the distribution volume of 3MG reached a constant value under each condition after 10 min of the tracer circulation, the apparent volume of distribution of 2DG, which increased with time in the awake mice, did not increase so greatly in the anesthetized and hypothermie mice, indicating that the net rate of 2DG phosphorylation is lowered under these conditions. These data were fitted for the conventional three-compartmental model and the values of rate constants of influx (K 1 ), efflux (k 2 ), phosphorylation, and dephosphorylation for 2DG, and K 1 and k 2 for 3MG were computed by nonlinear least square regression method. No significant difference in the value of K 1 k 2 , the distribution volume of the precursor pool, was observed between 2DG and 3MG, indicating that 3MG can be used to estimate the distribution volume of unmetabolized 2DG in the brain. The values of GU calculated from the values of estimated parameters were not significantly different from those calculated from blood and tissue radioactivities obtained during 10 min after the injection of the tracers in both awake and PB-anesthetized mice. These findings indicate that the double tracer technique is useful for measuring GU in a short duration experiment.

Published
1990
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