20 results on '"Hanan M. Alharbi"'
Search Results
2. Evaluation of diethyl 4-(5-bromo-1H-indol-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3,5-dicarboxylate: synthesis, anti-corrosion potential, and biomedical applications
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F. M. Mashood Ahamed, M. Syed Ali Padusha, A. Mushira Banu, Swastika Maitra, Hanan M. Alharbi, Vinoth Kumarasamy, Daniel E. Uti, Popat Mohite, Athanasios Alexiou, and Iftikhar Ali
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Dihydropyridine ,Corrosion ,Mild steel ,Antimicrobial ,Antioxidant ,Free radical ,Chemistry ,QD1-999 - Abstract
Abstract The pursuit of advanced multifunctional compounds has gained significant momentum in recent scientific endeavours. This study is dedicated to elucidating the synthesis, rigorous characterization, and multifaceted applications—encompassing anti-corrosion, antimicrobial, and antioxidant properties—of Diethyl 4-(5-bromo-1H-indol-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3,5-dicarboxylate. The 1,4-dihydropyridine derivative was meticulously synthesized through a strategic reaction of ethyl acetoacetate, ammonium acetate, and 5-bromoindole-3-carboxaldehydein the ethanol medium at 60 C. Subsequent spectral validations were conducted using sophisticated techniques, namely FTIR, NMR, and Mass spectrometry, resulting in data that perfectly resonated with the hypothesized chemical structure of the compound. Its anti-corrosive potential was assessed on mild steel subjected to an aggressive acidic environment, employing comprehensive methodologies like gravimetric analysis, Tafel polarization, and EIS. Concurrently, its antimicrobial prowess was ascertained against a spectrum of bacterial and fungal pathogens viz., Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Pseudomonas, Candida albicansandAspergillusniger, leveraging the disc diffusion method and using Gentamicin as a reference standard.The empirical results illustrated a substantial decrement in corrosion rates with ascending concentrations of the organic compound, achieving an apex of anti-corrosive efficacy at 81.89% for a concentration of 2 × 103 M. Furthermore, the compound outperformed Gentamicin in antimicrobial screenings, manifesting superior efficacy against all tested pathogens. The antioxidant potential, quantified using the DPPH free radical scavenging assay against ascorbic acid as a benchmark, was found to have an IC50 value of 113.964 ± 0.076 µg/ml.This comprehensive investigation accentuates the paramount potential of the synthesized dihydropyridine derivative in diverse domains—from industrial applications as a corrosion inhibitor to therapeutic avenues given its pronounced antimicrobial and antioxidant capabilities. The compelling results obtained pave the way for expansive research and development initiatives cantered around this multifaceted compound. Graphical Abstract
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- 2024
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3. Nanotechnological synergy of mangiferin and curcumin in modulating PI3K/Akt/mTOR pathway: a novel front in ovarian cancer precision therapeutics
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Hanan M. Alharbi, Taha Alqahtani, Ali H. Alamri, Vinoth Kumarasamy, Vetriselvan Subramaniyan, and K. Suresh Babu
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ovarian cancer therapeutics ,therapeutic efficacy ,mangiferin ,curcumin ,PI3K/Akt/mTOR pathway ,synergistic interplay ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Background: Ovarian cancer, colloquially termed the “silent killer” among gynecological malignancies, remains elusive due to its often-asymptomatic progression and diagnostic challenges. Central to its pathogenesis is the overactive PI3K/Akt/mTOR signaling pathway, responsible for various cellular functions, from proliferation to survival. Within this context, the phytochemical compounds mangiferin (derived from Mangifera indica) and curcumin (from Curcuma longa) stand out for their potential modulatory effects. However, their inherent bioavailability challenges necessitate innovative delivery systems to maximize therapeutic benefits.Objective: This study seeks to synergize the merits of nanotechnology with the therapeutic properties of mangiferin and curcumin, aiming to bolster their efficacy against ovarian cancer.Methods: Employing specific nanotechnological principles, we engineered exosomal and liposomal nano-carriers for mangiferin and curcumin, targeting the PI3K/Akt/mTOR pathway. Molecular docking techniques mapped the interactions of these phytochemicals with key proteins in the pathway, analyzing their binding efficiencies. Furthermore, molecular dynamics simulations, spanning 100 nanoseconds, verified these interactions, with additional computational methodologies further validating our findings. The rationale for the 100 nanoseconds time span lies in its sufficiency to observe meaningful protein-ligand interactions and conformational changes. Notably, liposomal technology provided an enhancement in drug delivery by protecting these compounds from degradation, allowing controlled release, and improving cellular uptake.Results: Our computational investigations demonstrated notable binding affinities of mangiferin and curcumin: PI3K at −11.20 kcal/mol, Akt at −15.16 kcal/mol, and mTOR at −10.24 kcal/mol. The adoption of exosome/liposome-mediated delivery significantly amplified the bioavailability and cellular uptake of these nano-formulated compounds, positioning them as potential stalwarts in ovarian cancer intervention. A brief explanation of exosome/liposome-mediated delivery involves the use of these vesicles to encapsulate and transport therapeutic agents directly to the target cells, enhancing drug delivery efficiency and minimizing side effects.Conclusion: Addressing ovarian cancer’s intricacies, dominated by the erratic PI3K/Akt/mTOR signaling, mandates innovative therapeutic strategies. Our pioneering approach converges nanotechnological liposomal delivery with mangiferin and curcumin’s natural efficacies. This confluence, validated by computational insights, heralds a paradigm shift in ovarian cancer treatment. As our findings underscore the collaborative potential of these phytochemicals, it beckons further exploration in translational studies and clinical applications, ensuring the best intersection of nature and technology for therapeutic advantage.
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- 2024
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4. The immunomodulatory effects of probiotics and azithromycin in dextran sodium sulfate-induced ulcerative colitis in rats via TLR4-NF-κB and p38-MAPK pathway
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Shereen E. Elkholy, Shymaa Ahmad Maher, Noura R. Abd el-hamid, Heba A. Elsayed, Wael Abdou Hassan, Asmaa K.K. Abdelmaogood, Samar M. Hussein, Mariusz Jaremko, Samar Zuhair Alshawwa, Hanan M. Alharbi, and Samar Imbaby
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Ulcerative colitis ,Azithromycin ,Probiotics ,Dextran sodium sulphate (DSS) model ,P-38 MAPK ,Toll-Like Receptor (TLR4) ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Ulcerative colitis (UC), a chronic autoimmune disease of the gut with a relapsing and remitting nature, considers a major health-care problem. DSS is a well-studied pharmacologically-induced model for UC. Toll-Like Receptor 4 (TLR4) and its close association with p-38-Mitogen-Activated Protein Kinase (p-38 MAPK) and nuclear factor kappa B (NF-κB) has important regulatory roles in inflammation and developing UC. Probiotics are gaining popularity for their potential in UC therapy. The immunomodulatory and anti-inflammatory role of azithromycin in UC remains a knowledge need. In the present rats-established UC, the therapeutic roles of oral probiotics (60 billion probiotic bacteria per kg per day) and azithromycin (40 mg per kg per day) regimens were evaluated by measuring changes in disease activity index, macroscopic damage index, oxidative stress markers, TLR4, p-38 MAPK, NF-κB signaling pathway in addition to their molecular downstream; tumor necrosis factor alpha (TNFα), interleukin (IL)1β, IL6, IL10 and inducible nitric oxide synthase (iNOS). After individual and combination therapy with probiotics and azithromycin regimens, the histological architecture of the UC improved with restoration of intestinal tissue normal architecture. These findings were consistent with the histopathological score of colon tissues. Each separate regimen lowered the remarkable TLR4, p-38 MAPK, iNOS, NF-κB as well as TNFα, IL1β, IL6 and MDA expressions and elevated the low IL10, glutathione and superoxide dismutase expressions in UC tissues. The combination regimen possesses the most synergistic beneficial effects in UC that, following thorough research, should be incorporated into the therapeutic approach in UC to boost the patients’ quality of life.
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- 2023
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5. Lupenone, a wonder chemical obtained from Euphorbia segetalis to boost affinity for the transcriptional factor escalating drought-tolerance in Solanum Lycopersicum: A cutting-edge computational biology approach
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Sandip Debnath, Taha Alqahtani, Ali Alqahtani, Hanan M. Alharbi, and Shopnil Akash
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Medicine ,Science - Published
- 2023
6. Association of recurrent spontaneous abortion with polycystic ovarian syndrome under the influence of killer immunoglobulin like receptors
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Hanan M. Alharbi, Afrah F. Alkhuriji, Suliman Y. Alomar, Zainb A. Babay, Alaa A. Alnafjan, Hussah M. Alobaid, Wazirah G. Allharbi, and Lamjed A. Mansour
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Killer immunoglobulin like receptors ,Recurrent spontaneous abortion ,Ovarian polycystic syndrome ,HLA-C1 ,HLA-C2 ,Natural killer cell ,Science (General) ,Q1-390 - Abstract
Aims: The primary aim of the current study is to decide if genetic polymorphisms of the killer immunoglobulin-like receptor (KIR) and human leukocyte antigen (HLA-C) genes are related to recurrent spontaneous abortion (RSA) in the Saudi women who are affected with polycystic ovarian syndrome (PCO) and in PCO patients without RSA. Materials and methods: The study was conducted in Riyadh and included 199 cases divided as follows: 69 PCO Saudi women with an RSA history, i.e. three or more spontaneous abortion cases, 65 patients were exclusively with PCO and 65 healthy controls, typed for 17 KIR genes and the HLA-C1 and HLA-C2 allotypes using polymerase chain reaction-sequence-specific primer methodology. Results: The study reported that the recurrences of KIR3DL1, 2DS4ins, 2DL2, and KIR2DS2 significantly reduced among RSA-PCO women in comparison with healthy controls (OR = 337.45, 39.08, 2.4, 4.59 with p
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- 2022
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7. Antioxidant and Gastroprotective Activity of Suaeda fruticosa Forssk. Ex J.F.Gmel
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Afsheen Ayaz, QurratUlAin Jamil, Musaddique Hussain, Fayyaz Anjum, Adeel Sarfraz, Taha Alqahtani, Nadia Hussain, Reem M. Gahtani, Ayed A. Dera, Hanan M. Alharbi, and Shahid M. Iqbal
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gastroprotective ,antioxidant ,Suaeda fruticosa ,mucin contents ,Organic chemistry ,QD241-441 - Abstract
Suaeda fruticosa Forssk. Ex J.F.Gmel is traditionally used for inflammatory and digestive disorders, as a carminative, and for diarrhea. This plant is widely distributed in Asia, Africa, and the Mediterranean region. Aqueous methanolic extract of S. fruticosa (Sf.Cr) was prepared and screened for phytoconstituents through qualitative and GC-MS analysis. Quantification of total phenolic and flavonoid contents was performed, while antioxidant capacity was determined by DPPH, CUPRAC, FRAP, and ABTS assays. The gastroprotective activity was assessed in an ethanol-induced ulcer model. Gastric secretory parameters and macroscopic ulcerated lesions were analyzed and scored for ulcer severity. After scoring, histopathology was performed, and gastric mucus contents were determined. Oral pre-treatment of Sf.Cr demonstrated significant gastroprotection. The gastric ulcer severity score and ulcer index were reduced while the %-inhibition of ulcer was increased dose-dependently. The Sf.Cr significantly elevated the pH of gastric juice, while a decrease in total acidity and gastric juice volume was observed. Histopathology demonstrated less oedema and neutrophil infiltration in gastric mucosa of rats pre-treated with the Sf.Cr in comparison to ethanol-intoxicated animals. Furthermore, the gastric mucus contents were increased as determined by alcian blue binding. Sf.Cr showed marked gastroprotective activity, which can be attributed to antioxidant, antisecretory, and cytoprotective effects.
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- 2022
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8. Pharmacological Validation for the Folklore Use of Ipomoea nil against Asthma: In Vivo and In Vitro Evaluation
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Taha Alqahtani, Sajida Parveen, Yahia Alghazwani, Hanan M. Alharbi, Reem M. Gahtani, Nadia Hussain, Kashif ur Rehman, and Musaddique Hussain
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Ipomoea nil seeds ,bronchodilator ,anti-asthmatic ,IgE antibody ,inflammatory cells ,hyper-responsive ,Organic chemistry ,QD241-441 - Abstract
Oxidative stress is the key factor that strengthens free radical generation which stimulates lung inflammation. The aim was to explore antioxidant, bronchodilatory along with anti-asthmatic potential of folkloric plants and the aqueous methanolic crude extract of Ipomoea nil (In.Cr) seeds which may demonstrate as more potent, economically affordable, having an improved antioxidant profile and providing evidence as exclusive therapeutic agents in respiratory pharmacology. In vitro antioxidant temperament was executed by DPPH, TFC, TPC and HPLC in addition to enzyme inhibition (cholinesterase) analysis; a bronchodilator assay on rabbit’s trachea as well as in vivo OVA-induced allergic asthmatic activity was performed on mice. In vitro analysis of 1,1-Diphenyl-2-picrylhydrazyl radical (DPPH) expressed as % inhibition 86.28 ± 0.25 with IC50 17.22 ± 0.56 mol/L, TPC 115.5 ± 1.02 mg GAE/g of dry sample, TFC 50.44 ± 1.06 mg QE/g dry weight of sample, inhibition in cholinesterase levels for acetyl and butyryl with IC50 (0.60 ± 0.67 and 1.5 ± 0.04 mol/L) in comparison with standard 0.06 ± 0.002 and 0.30 ± 0.003, respectively, while HPLC characterization of In.Cr confirmed the existence with identification as well as quantification of various polyphenolics and flavonoids i.e., gallic acid, vanillic acid, chlorogenic acid, quercetin, kaempferol and others. However, oral gavage of In.Cr at different doses in rabbits showed a better brochodilation profile as compared to carbachol and K+-induced bronchospasm. More significant (p < 0.01) reduction in OVA-induced allergic hyper-responses i.e., inflammatory cells grade, antibody IgE as well as altered IFN-α in airways were observed at three different doses of In.Cr. It can be concluded that sound mechanistic basis i.e., the existence of antioxidants: various phenolic and flavonoids, calcium antagonist(s) as well as enzymes’ inhibition profile, validates folkloric consumptions of this traditionally used plant to treat ailments of respiration.
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- 2022
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9. Nano-formulations composed of cell membrane-specific cellular lipid extracts derived from target cells: physicochemical characterization and in vitro evaluation using cellular models of breast carcinoma
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Hanan M. Alharbi and Robert B. Campbell
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Breast cancer ,Cell lines ,Cholesterol ,Liposomes ,Drug delivery systems ,Therapeutics. Pharmacology ,RM1-950 ,Pharmacy and materia medica ,RS1-441 - Abstract
Abstract Highly selective drug targeting is an important goal in the development of cancer nanotechnologies. In an effort to improve tumor targeting a method was developed to formulate cell membrane lipid-extracted nanoliposomes (CLENs). The main ingredients were extracted directly from the membrane of cancer cells. For this study we used three different breast cancer cell lines (4 T1, BT-20, and SK-BR-3). As controls for the normal breast and cancer tissue environments we employed the normal breast fibroblast (CRL-2089) and ovarian cancer (SK-OV-3) cell lines, respectively. We evaluated physicochemical properties, efficiency of drug loading, cellular uptake, and cytotoxicity. The mean diameter and zeta potential values for the 5 different CLENs were 202 ± 38 nm and − 15 ± 3.8 mv, respectively. Doxorubicin hydrochloride (5 mol%) increased the size of 4 T1-CLENs from 158 ± 2 nm to 212 ± 59 nm, with no significant change in the negatively-charged surface potential. Percent of drug loaded ranged from 40 to 93%, varying according to the ratio of lipid extract to conventional components employed. The additional inclusion of cholesterol and DPPE-PEG5000 increased drug loading in CLENs, similar to Doxil preparations. The most promising cellular uptake and cytotoxicity profiles were observed when the lipid ingredients were derived from the eventual target cell. Given the ability of CLENs to better recognize target cells compared to nanosystems consisting of non-specific lipid extracts or conventional liposome ingredients alone, CLENs has demonstrated early promise as a nano-delivery system for cancer treatment.
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- 2018
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10. Enhancing the Dissolution of Oral Dasatinib Tablets Using Zein–Hydroxypropyl Methylcellulose Solid Dispersions
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Hanan M. Alharbi, Taha Alqahtani, Afnan Batubara, Aisha Alshaer, Bushra Alqurashi, Lama Bahwairth, Huda Khawaji, and And Majd Almohammadi
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General Medicine - Published
- 2023
11. GC-MS analysis of Taraxacum officinale flowers and investigation of antimicrobial, anti-pellicle & anti-biofilm activities
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Hoor Shumail, Tahira Hassan, null Barkatullah, Shah Khalid, Naveed Akhtar, Ali Al-Qahtani, Afaf Aldahish, Taha Alqahtani, Hanan M. Alharbi, and Reem M. Gahtani
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Inorganic Chemistry ,Organic Chemistry ,Materials Chemistry ,food and beverages - Abstract
Plants synthesize large amount of useful and complex products which have no obvious metabolic and growth functions. These complex materials are said to be as secondary metabolites—phytochemicals which are plants active compounds possessing the potential to inhibit diseases. The purpose of the recent study was to investigate the pharmaceutical values of the flowers of Taraxacum officinale, for antimicrobial, anti-pellicle and anti-biofilm properties. Metanolic extracts with chloroform and n-hexane fractions against selected different bacterial (E.coli, P.aeruginosa, S.aureus, S.typhi) and fungal (F.oxysporum, A.niger, A.alternata, A.Terreus) strains were tested and GC-MS, FTIR and HPLC techniques, for detection of various secondary metabolites which are responsible for these activities, were performed. In antimicrobial assay, the result of the methanolic extract and fractions of the flowers was found to be effective against the tested bacterial and fungal strains. The GC-MS and FTIR analysis of chloroform fractions of T. officinale flowers reported the presence of a wide range of phytochemicals and secondery metabolites liable for the biological activities that can be purified in future for the synthesis of noval improved and valuable pharmaceutical products.
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- 2022
12. Natural Products and Their Promise Against COVID 19: Review
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Abeer Temraz, Fatma Abo-Elghiet, and Hanan M. Alharbi
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Engineering ,Nutrition and Dietetics ,Coronavirus disease 2019 (COVID-19) ,business.industry ,Public Health, Environmental and Occupational Health ,food and beverages ,Biochemical engineering ,business ,Natural (archaeology) ,Food Science - Abstract
Background: Since the beginning of medical history, plants have been exemplary sources of a variety of pharmacological compounds that are still used in modern medication. Respiratory infections are a serious and persistent global health problem, most acute and chronic respiratory infections are caused by viruses, whose ability to mutate rapidly may result in epidemics and pandemics, as seen recently with MERS-COV (2012) and SARS-COV-2 (2019), the latter causing coronavirus disease 2019 (COVID-19). Methods: This study aims to highlight the tremendous benefits of plants that have been widely used as dietary supplements or traditional treatment for various respiratory infections, with a focus on the most effective constituents and studies that revealed their activities against COVID-19. Results: Several traditional plants and their phytoconstituents have shown activity against respiratory viruses, including SARS-COV-2. The presented plants are Nigella sativa, Punica granatum, Panax ginseng, Withania somnifera, Glycyrrhiza glabra, Curcuma longa, Zingiber officinale, Camellia sinensis, Echinacea purpurea, Strobilanthes cusia, Stephania tetrandra, and genus Sambucus. Conclusion: The data discussed in this review can encourage carrying out in-vivo studies that may help in the discovery of herbal leads that can be feasibly used to alleviate, prevent or treat COVID-19 infection.
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- 2022
13. Chitosan nanocomposite film incorporating Nigella sativa oil, Azadirachta indica leaves’ extract, and silver nanoparticles
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Afnan S. Batubara, Najla A. Obaid, Hanan M. Alharbi, Turki Mohammed Altalhi, Mohammed Abdullah Alasmari, Ammar Zuhair Alghamdi, and Eram Sharmin
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Polymers and Plastics ,General Chemical Engineering ,Physical and Theoretical Chemistry - Abstract
Nanocomposite films have been prepared from chitosan, cinnamaldehyde, Nigella sativa or blackseed oil, and silver nanoparticles (NPs) biosynthesized in Azadirachta indica or neem leaves’ extract. The methodology involved simple blending of components through “green chemistry” route. The films obtained were soft and foldable. The morphology by scanning electron microscopy confirmed the inclusion of NPs in the films. Microbial penetration study demonstrated that the films offered good resistance to secondary bacterial infection. The antibacterial study against Staphylococcus aureus (ATCC 29213) and E. coli (ATCC 25922) indicated moderate antibacterial behavior of the films. The swelling behavior in water, phosphate buffer saline, and simulated wound fluid was found to be appropriate for use as wound dressings. The films were biodegradable in soil and showed good thermal stability up to 200°C.
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- 2023
14. Influence of Open Apex on Working Length Determination Using Cone-Beam Computed Tomography and Apex Locators: A Comparative In Vitro Study
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Swati Srivastava, Hanan M. Alharbi, Mai Soliman, Elzahraa Eldwakhly, and Manal M. Abdelhafeez
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Article Subject ,General Immunology and Microbiology ,Tooth Apex ,Humans ,Odontometry ,Bicuspid ,General Medicine ,Cone-Beam Computed Tomography ,Dental Pulp Cavity ,General Biochemistry, Genetics and Molecular Biology ,Root Canal Preparation - Abstract
Objectives. The aim of this study is to assess the effect of open apex on working length (WL) determination with aid of cone-beam computed tomography (CBCT) and electronic apex locators (EALs). Methods. Thirty-two extracted human mandibular premolars were selected, and apical 5 mm was removed. Root canals were prepared from the apical to the coronal direction of the canal using peeso reamers up to size 5 (retrograde) to simulate open apex. The samples were analyzed by CBCT, and WL was established (CWL) by a radiologist. An endodontist prepared the access cavities, and visual working length (VWL) was recorded. The samples were embedded in a freshly mixed alginate mould up to cementoenamel junction. Each root canal length was measured with two different EALs—Root ZX mini and i Root. The measurements were repeated 3 times by using a digital caliper, and the mean was recorded by the endodontist who was blinded to the results of the CWL. The recorded data was statistically analyzed using the SPSS software. Results. The results of this study showed statistically significant difference between VWL and i Root, CWL and i Root, and Root ZX mini and i Root ( p < 0.05 ). Amongst EALs, a superior accuracy was noted for Root ZX mini than as compared to i Root. However, no statistically significant difference was seen between Root ZX mini and CWL ( p > 0.05 ). Conclusion. The present study showed that CWL is as accurate and reliable as VWL which is the gold standard. Amongst EALs, Root ZX mini performed more accurately than i Root. Preexisting CBCT scans should be used as an advantage in determining WL.
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- 2022
15. Assessment of the Proximity of the Inferior Alveolar Canal with the Mandibular Root Apices and Cortical Plates—A Retrospective Cone Beam Computed Tomographic Analysis
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Swati Srivastava, Hanan M. Alharbi, Afnan S. Alharbi, Mai Soliman, Elzahraa Eldwakhly, and Manal M. Abdelhafeez
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cone beam computed tomography ,cortical plates ,endodontic mishaps ,iatrogenic error ,inferior alveolar canal ,inferior alveolar nerve ,mandibular teeth ,neuropathy ,over-instrumentation ,paresthesia ,proximity ,root apex ,Medicine (miscellaneous) - Abstract
Various endodontic interventions often lead to iatrogenic damage to the inferior alveolar nerve present in the inferior alveolar canal (IAC). The purpose of the present study was to analyze the relationships of IAC with the root apices of mandibular teeth and with the mandibular cortical plates. Materials: 116 cone beam computed tomography (CBCT) scans were examined and the shortest distance of IAC with the root apices of mandibular canines, premolars and molars, and with cortical plates was analyzed. The data were statistically analyzed using SPSS. Results: The shortest mean distance between IAC and lingual cortical plate (LCP) was found in the third molar area, and between IAC and buccal cortical plate (BCP) in the second premolar area. A high incidence of 60% direct communication (DC) was present in mandibular second molars; 38% in mandibular third molars; 13% in mandibular second premolars; 12% in mandibular first molars; and 1% in mandibular first premolars. Conclusion: Anteriorly, IAC was found to be significantly present in close approximation to the roots of mandibular canines. Posteriorly, IAC was found to be in significant proximity to the distal roots of mandibular second molars.
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- 2022
16. Personalized Nanoparticles for Cancer Therapy: A Call for Greater Precision
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Shye Richardson, Lee Christopher, Robert B. Campbell, Valery Forcha-Etieundem, Nare Sahakyan, Amir Haddad, and Hanan M. Alharbi
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0301 basic medicine ,Pharmacology ,Cancer Research ,medicine.medical_specialty ,business.industry ,Cancer therapy ,Nanotechnology ,Cancer treatment ,Clinical trial ,03 medical and health sciences ,030104 developmental biology ,Neoplasms ,Drug delivery ,Cancer drug delivery ,Humans ,Nanoparticles ,Molecular Medicine ,Nanomedicine ,Medicine ,Medical physics ,Personalized medicine ,Precision Medicine ,Disease management (health) ,business - Abstract
Nanotechnology has brought about the advent of personalized medicine in the era of targeted therapeutic strategies for cancer therapy. The ability to exploit tumor features for therapeutic gain has made it possible to manufacture more effective nanomedicines for cancer treatment. However, known obstacles, including the inability to overcome pathophysiological barriers of tumors, have impeded disease management. In spite of this, recent efforts have been made to develop more functionalized nanosystems that utilize the active-targeting approach. This article reviews the FDA-approved cancer drug delivery systems in the general framework of personalized nanomedicine. We discuss the latest efforts in the development of functionalized nano-systems, and summarize relevant ongoing preclinical and clinical trials.
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- 2017
17. Abstract 4666: An evaluation of breast cancer cellular membrane lipid-extracted nanoliposomes (CLENs) in relation to formulation design, stability, mechanism of cellular entry and cardioprotective function in vitro
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Robert B. Campbell and Hanan M. Alharbi
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Cancer Research ,Liposome ,Chemistry ,Cell ,Cancer ,Pharmacology ,medicine.disease ,In vitro ,Cell membrane ,medicine.anatomical_structure ,Oncology ,Targeted drug delivery ,medicine ,Nanocarriers ,Cytotoxicity - Abstract
One of the longstanding issues limiting the use of chemotherapeutic agents is the lack of tumor specificity, which leads to systemic toxicity. Therefore, various targeted nanocarriers have been developed to concentrate the cytotoxic drug agents at tumor regions to avoid uptake by normal healthy tissues. Previous studies from our laboratory have demonstrated that cell membrane lipid-extracted nanoliposomes (CLENs) are capable of selective targeting compared to nanoliposomes consisting of mainly conventional lipid materials. Also, CLENs were relatively nontoxic when employed at concentrations traditionally used to evaluate nanoparticles in vitro. However, parameters such as lipid composition, size, and surface charge all have a direct impact on liposomal cellular uptake. In this study, we prepared different CLENs different molar ratios of natural and cellular-derived lipids extracted directly from breast cancer cells (4T1), cholesterol, and DPPE-PEG-5000. The formulations were used to investigate the mechanisms of CLENs uptake and determine the underlying mechanisms regulating cellular entry. In the study, CLENs were used to evaluate cytotoxicity and cellular uptake for both target and off-target cell populations. CLENs containing cholesterol and DPPE-PEG-5000 (70/25/5) were able to retain doxorubicin in relevant therapeutic concentrations. CLEN formulations were able to exert selective cytotoxic drug effects against different target breast cancer cells in vitro. 4T1 CLENs containing cholesterol and DPPE-PEG-5000 (70/25/5) demonstrated greater binding to 4T1 (target) cells compared to CLENs prepared using 4T1 lipid extracts alone. The binding was not only temperature- and time-dependent, but also composition- and cell type-dependent. Off-target effect studies showed that 4T1 CLENs were taken up minimally by off-target cells (including normal breast fibroblasts and normal myocytes), compared to controls. Our studies collectively support the use of lipid extracts derived from target cells for selective drug targeting and enhanced cytotoxicity. Future studies will explore underlying mechanisms of cellular entry and cardioprotective function. Citation Format: Hanan M. Alharbi, Robert B. Campbell. An evaluation of breast cancer cellular membrane lipid-extracted nanoliposomes (CLENs) in relation to formulation design, stability, mechanism of cellular entry and cardioprotective function in vitro [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2018; 2018 Apr 14-18; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2018;78(13 Suppl):Abstract nr 4666.
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- 2018
18. Abstract 3876: Development and in vitro evaluation of cell membrane lipid-extracted nanoliposomes for lung cancer treatment
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Aarohi Bhatt, Robert K. Campbell, Jasmine King, Hanan M. Alharbi, Thomas Preteroti, and Drew Goodrich
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Cell membrane ,Cancer Research ,medicine.anatomical_structure ,Oncology ,Chemistry ,medicine ,Cancer research ,Lung cancer ,medicine.disease - Abstract
Background: Every year more deaths are attributed to lung cancer compared to colon, prostate, and breast cancers combined. Lung cancer is typically diagnosed at late stages, and has demonstrated poor treatment outcomes due to acquired drug resistance. Unfortunately, as one method is introduced to overcome drug resistance a new mechanism can arise. In an effort to overcome drug resistance lipid extracts (LE) were derived from target cells, and the ingredients were used to develop nanoliposomes that would selectively target a desired cell population compared to controls. The early studies were designed to demonstrate the use of CLENs (cellular membrane lipid-extracted nanoliposomes) for the targeting of lung cancer. Experimental Procedures: CLENs were initially prepared using various ratios of LE/DOPC/Chol/PEG by thin film evaporation. Particle size and zeta potential values were determined for all preparations of CLENs (with and without drug). CLENs were evaluated for selectivity for target compared to control cells. Cytotoxicity studies were performed on various doxorubicin hydrochloride-loaded CLENs using a cell model of lung cancer (ChagGo-K-1). For cellular uptake studies ChaGo-K-1 cells were seeded at 10,000 cells/mL in a 48-well plate and allowed to incubate for 24 hours prior to cell studies. A fluorescence microplate reader was used to determine fluorescence intensity values for the two studies. Results: ChaGo-K-1 CLENs demonstrated an average particle size of 206+/-2 nm and zeta-potential of -21+/-3 mV, illustrating favorable characteristics for drug delivery and interstitial drug targeting. The cellular uptake studies suggested that ChaGo-K-1 CLENs were selective for ChaGo-K-1 cells in vitro, when compared to all other control varieties of CLENs. Drug-loaded ChaGo-K-1 CLENs displayed significant cytotoxicity against ChaGo-K-1 cells compared to control cells. Fluorescence microscopy revealed association of ChagGo-K-1 CLENs with target cells. Conclusions: Based on promising early finding CLENs may represent a relatively tumor specific drug delivery system that can be used to overcome drug resistance, improve efficacy, and to limit off-target drug effects. Preclinical studies will investigate whether the relative selectivity and cytotoxicity observed against lung cancer cells in vitro translates to favorable tumor uptake and efficacy in vivo. Citation Format: Jasmine King, Aarohi Bhatt, Drew Goodrich, Thomas Preteroti, Hanan Alharbi, Robert Campbell. Development and in vitro evaluation of cell membrane lipid-extracted nanoliposomes for lung cancer treatment [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2018; 2018 Apr 14-18; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2018;78(13 Suppl):Abstract nr 3876.
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- 2018
19. Abstract 3881: Formulating a cell membrane lipid-extracted nanoliposome (CLENS) drug platform for the treatment of prostate cancer
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Ali Alqarni, Abubker Omaer, Carlos Randulfe, Robert B. Campbell, and Hanan M. Alharbi
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010302 applied physics ,Cancer Research ,Liposome ,Chromatography ,Chemistry ,Cancer ,02 engineering and technology ,021001 nanoscience & nanotechnology ,medicine.disease ,01 natural sciences ,Cell membrane ,medicine.anatomical_structure ,Oncology ,0103 physical sciences ,Cancer cell ,Drug delivery ,medicine ,Doxorubicin Hydrochloride ,Doxorubicin ,0210 nano-technology ,Cytotoxicity ,medicine.drug - Abstract
Background: Liposome-encapsulated doxorubicin has proven to be an effective drug delivery method to cancer cells. Lipid extracts derived from human prostate cancer cells along with conventional components were employed to develop cell membrane lipid-extracted nanoliposomes (CLENs) for prostate cancer treatment. In an effort to balance efficiency of drug loading with cellular targeting efficiency we report on physicochemical characteristics (i.e., particle size, zeta potential, drug loading efficiency) and cellular uptake studies. Methods: Five preparations of CLENS were prepared consisting of variable concentrations of DOPC and CRL-1740 lipid extract material varying as a function of optimized fixed ratios of conventional components (Cholesterol and DSPE-PEG5000). The controls used to investigate the effect of the different components on drug incorporation efficiency were preparations of DOPC (100%) and CRL-1740 (100%). Doxorubicin hydrochloride (5 mol%) was employed during the preparation of all formulations. Samples were readily prepared by thin film evaporation. For cellular uptake studies CRL-1740 cells were seeded at 1 x 104 mL in 48-well plate and incubated for 24 hours prior to use. The cells were next exposed to various amounts of rhodamine labeled CLENS for 24 hrs. Cellular uptake was determined using fluorescence intensity values determined by a fluorescence microplate reader. Fluorescence microscopy supplement cellular uptake studies. Results: The DOPC drug-loaded liposome preparation consistently achieved the lowest percent of drug incorporation. Likewise, 100% CRL-1740 CLENs demonstrated a significant degree of formulation instability, resulting in a relatively low percent of drug incorporation compared to preparations that consisted of lipid extract and conventional components. The finding were supported by values determined for zeta potential and particle size. Cellular uptake studies also favored the preparations that consisted of lipid extract material and conventional components compared to each alone. The results collectively support the importance of balancing lipid extract material derived from prostate cancer target cells with cholesterol and DSPE-PEG5000 for formulation development and cellular uptake. Conclusion: We formulated seven preparations of CLENS as part of the drug platform. The most promising candidates were determined following completion of the study. Future studies should evaluate the cytotoxicity and safety profile of the most promising formulations vivo. Citation Format: Abubker Omaer, Ali Alqarni, Carlos Randulfe, Hanan Alharbi, Robert Campbell. Formulating a cell membrane lipid-extracted nanoliposome (CLENS) drug platform for the treatment of prostate cancer [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2018; 2018 Apr 14-18; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2018;78(13 Suppl):Abstract nr 3881.
- Published
- 2018
20. Abstract 5513: Employing a unique panel of breast cancer cellular lipids extracts in the development of a novel nanoliposome drug platform
- Author
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Hanan M. Alharbi and Robert B. Campbell
- Subjects
Drug ,Cancer Research ,Biodistribution ,Liposome ,business.industry ,media_common.quotation_subject ,Cell ,Cancer ,medicine.disease ,medicine.anatomical_structure ,Breast cancer ,Oncology ,Pharmacokinetics ,Immunology ,Drug delivery ,medicine ,Cancer research ,business ,media_common - Abstract
Breast cancer is a major cause of cancer death in women worldwide. Breast cancer is a heterogeneous disease characterized by variant pathological features, disparate responses to therapeutics, and substantial differences in patient's long-term survival. The use of drug delivery systems such as liposomes can favorably alter the pharmacokinetic and biodistribution profile of commonly used drugs used to treat breast cancer disease. An important feature of a drug delivery system is its ability to recognize and selectively target tumor cells with relatively high affinity when compared to healthy tissues. For this reason, the goal of this study was to develop a novel nanoliposome system that would target tumor cells to a significantly greater extent when compared to more conventional liposome systems. In this study, natural lipids extracted directly from breast cancer cells were used to prepare cellular lipid-extracted nanoliposomes (CLENs). The rationale is that an array of different lipids with a unique composition profile would represent a more relevant source of lipid material when compared to more traditional methods of liposome preparation. We therefore investigated whether the inclusion of extracted lipids derived from breast cancer cells would improve the selective uptake of liposomes by breast cancer cells, and related formulation features. To achieve these goals, three breast cancer cell lines were used (4T1, BT-20, and SK-BR-3), a normal breast fibroblast (CRL-2089), and an ovarian cancer cell line (SK-OV-3- used as a negative control). The size of CLENs fell within the size range of 120- 203 nm and possessed a negatively-charged liposome surface charge potential (ranging between -10.97 and -20.97 mV). The nanoliposomes were also non-toxic to cells within the concentration range evaluated (up to 1μmol). Furthermore, without exception, the cellular uptake of CLENs was greatest when the extracted material used to prepare CLENs was the same as the target cell from which the lipids were extracted. In addition, when the breast cancer cell lines (4T1, SK-BR-3 and BT-20) were used to prepare CLENs, SK-OV-3 and CRL-2089 cell lines took up CLENs to a lesser extent, suggesting a more selective and differential cellular uptake. In conclusion, the novel nanoliposome platform represents a promising drug delivery alternative to more conventional nano drug delivery systems. Additional formulation studies are currently underway. Citation Format: Hanan M. Alharbi, Robert B. Campbell. Employing a unique panel of breast cancer cellular lipids extracts in the development of a novel nanoliposome drug platform. [abstract]. In: Proceedings of the 106th Annual Meeting of the American Association for Cancer Research; 2015 Apr 18-22; Philadelphia, PA. Philadelphia (PA): AACR; Cancer Res 2015;75(15 Suppl):Abstract nr 5513. doi:10.1158/1538-7445.AM2015-5513
- Published
- 2015
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