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Objective: This study aimed to explore the prevalence of prenatal depression and to determine the dyadic relationship between perceived stress, marital satisfaction, and prenatal depression in pregnant women with gestational diabetes mellitus (GDM) and their partners., Background: GDM during pregnancy is a very stressful event for couples. However, previous studies have largely focused on pregnant women, with few including their partners., Methods: A cross-sectional study was conducted in Guangzhou, China, from 30 July to 2 December 2021. Three hundred fourteen couples with GDM completed the Perceived Stress Scale, Locke-Wallace Marital Adjustment Scale, and Edinburgh Postnatal Depression Scale. Dyadic analysis was conducted using the actor-partner interdependence mediation model., Results: The prevalence of depressive symptoms was 13.4% in pregnant women with GDM and 8.3% in their partners. Regarding the actor effects, perceived stress was positively associated with prenatal depression in pregnant women with GDM and their partners, respectively, and marital satisfaction acted as a mediating role. Regarding the partner effects, paternal perceived stress was negatively associated with maternal marital satisfaction, and maternal marital satisfaction mediated the association between paternal perceived stress and maternal prenatal depression., Conclusions: Dyadic effects of perceived stress, marital satisfaction, and prenatal depression exist in couples with GDM.

Aims: This study aims to examine the prevalence of anxiety symptoms and identify predictors of anxiety among pregnant women with gestational diabetes mellitus and their partners and explore the mediating role of marital satisfaction between maternal and paternal anxiety., Design: A cross-sectional study was conducted in Guangzhou, China, from July 2021 to May 2022., Methods: A total of 306 dyads of pregnant women with gestational diabetes mellitus and their partners completed the State-Trait Anxiety Inventory, Locke-Wallace Marital Adjustment Test and the socio-demographic and clinical data sheet., Results: The prevalence of anxiety symptoms was 32.4% and 36.6% in pregnant women with gestational diabetes mellitus and their partners, respectively. The predictors of maternal anxiety were paternal anxiety, maternal marital satisfaction, maternal monthly salary, fasting glucose value and 1-h glucose value. By contrast, the predictors of paternal anxiety were maternal anxiety, paternal marital satisfaction and paternal monthly salary. Moreover, the relationship between maternal and paternal anxiety was mediated by marital satisfaction., Conclusions: The anxiety symptoms of pregnant women with gestational diabetes mellitus and their partners influence each other, and this relationship was mediated by marital satisfaction. Every couple should be screened for anxiety symptoms and treated as a team rather than focusing solely on the pregnant woman., (© 2023 John Wiley & Sons Australia, Ltd.)

Brain organoids have been widely used to study diseases and the development of the nervous system. Many reports have investigated the application of brain organoids, but most of these models lack vascular structures, which play essential roles in brain development and neurological diseases. The brain and blood vessels originate from two different germ layers, making it difficult to induce vascularized brain organoids in vitro. We developed this protocol to generate brain-specific blood vessel and cerebral organoids and then fused them at a specific developmental time point. The fused cerebral organoids exhibited robust vascular network-like structures, which allows simulating the in vivo developmental processes of the brain for further applications in various neurological diseases. Key Features • Culturing vascularized brain organoids using human embryonic stem cells (hESCs). • The new approach generates not only neural cells and vessel-like networks but also brain-resident microglia immune cells in a single organoid., Competing Interests: Competing interestsThe authors declare no competing interests., (©Copyright : © 2023 The Authors; This is an open access article under the CC BY license.)

Multifunctional materials are an attractive research area. Organic-inorganic hybrid perovskites are widely used in the design of these materials due to their rich properties and flexible composition. It is easy to obtain more photoelectric properties by introducing chiral groups as ligands. In this work, we synthesized chiral one-dimensional organic-inorganic hybrid perovskites, namely (R/S-3-HP)PbBr 3 (1R/1S) (3-HP=3-hydroxy-piperidine). The enantiomer compounds undergo reversible phase transition at 349/336 K. Under the excitation light of 339 nm, 1R and 1S have a wide emission peak at 635 nm, showing orange light. In addition, the indirect bandgap is 3.29 eV and the SHG intensity is comparable to that of KDP. This work provides a way to design multifunctional chiral perovskite materials., (© 2022 Wiley-VCH GmbH.)

In this work, the single crystal to single crystal (SCSC) transformations in three mononuclear copper complexes [Cu L 2 2 ]Cl 2 ·2H 2 O ( 1 ), [Cu L 1 2 Cl 2 ] ( 2 ), and [Cu L 2 2 ]Cl 2 ·4H 2 O ( 3 ) ( L 1 = di-2-pyridyl ketone, L 2 = di(pyridin-2-yl)methanediol) are realized by the irreversible dehydration and hydration reaction of L 1 and L 2 . Dark purple crystal 1 is obtained by self-assembly of L 1 and CuCl 2 ·2H 2 O in solvothermal reactions, in which the carbonyl group of L 1 undergoes a hydration addition reaction to form L 2 . On heating, 1 transforms to 2 by dehydrating water accompanied by the change of the color and coordination octahedron of Cu II ions. In a saturated water vapor environment, 2 can absorb six water molecules and transform to 3 with the same color and coordination environment with 1 but different lattice water. The SCSC process from 2 to 3 is reversible: 3 can transform back to 2 on heating like that of 1 . Chroma rewritable behaviors in the structural transformation of the complexes make them visually identifiable temperature or water probes.

Multifunctional materials have always been an attractive research area, but how to combine multiple excellent properties in one compound remains a considerable challenge. Organic-inorganic hybrid compounds are widely used in the design of such materials due to their rich properties and flexible assembly. Herein, two new manganese(II)-based organic-inorganic hybrid compounds, (C 6 NH 16 ) 2 MnBr 4 ( 1 ) and (C 7 NH 18 ) 2 MnBr 4 ( 2 ), are prepared by the solution method. Compounds 1 and 2 both emit extremely strong green light under UV excitation, with high quantum yields of 45.93 and 50.98%, respectively. In addition, reversible solid-state phase transitions and obvious switchable dielectric properties are shown at 378/366 and 361/352 K, respectively. The coexistence of the dual stimulus-response characteristics of temperature and light in compounds 1 and 2 opens a new path for exploring more multifunctional phase transition materials.

Objective: To study the response characteristics of the posterior intralaminar nucleus (PIN) of auditory thalamus in VGluT2-Cre transgenic adult mice when exposed to white noise and 10K pure tone stimulation. Methods: All adult male Vglut2-Cre mice (8-12 weeks) were used in this study between Oct, 2017 and Oct, 2018. Using the calcium signal fiber photometry method, optic fiber was employed to locate on PIN by injecting AAV-hSyn-DIO-GCaMP6m virus, and thereafter, the activity of the target cluster neurons during different acoustic stimuli was recorded. Matlab was used for data processing and statistical analysis. Results: (1)In both white noise and 10 kHz pure tone as a continuous three-second stimulation, the peak amplitude of calcium signal activity generated in PIN by white noise was superior to that of pure tone, the statistic result showed significantly difference ( n= 6, t= 2.404, P= 0.037 1) . (2)In addition, when white noise and 10K pure tone played as consecutive 3 or 5 pips within three-second stimulation, the stimulus-following ability in a consecutive 3 pulses play within 3 seconds was far better than a consecutive 5 pips play within 3 seconds (in both white noise and 10 kHz pure tone), yet consecutive 3 pips play showed greater signal attenuation speed than that in consecutive 5 pips play, the statistic result showed significantly difference ( n= 6, t= 2.748 P= 0.033 4) .(3)Regardless of the intra-group comparisons between white noise and 10 kHz pure tone stimulation, PIN showed better signal response in a consecutive 3 pips play than consecutive 5 pips play or a continuous three-second stimulation. When came to the statistical analysis, the acoustic response degree of a continuous three-second stimulation was an intermediate between two others, both consecutive 3 or 5 pips play showed significantly difference. Conclusions: The results suggest that under the same acoustic intensity, VGluT2-Cre transgenic adult mice's PIN shows greater signal response in white noise than pure tone. PIN shows greater signal attenuation to repetition play of 10 kHz pure tone, which implies PIN shows stronger adaptation to 10 kHz pure tone than to white noise. Lastly, PIN is more responsive to a complex sound information (white noise) than to simple sound information (pure tone).

Our bioassays reviewed that antennae played crucial roles in the responses of maize weevil (Sitophilus zeamais) to food and sex volatiles. In order to identify the maize weevil odorant-binding protein (OBP) genes, we analyzed its antennal transcriptome. In total, 21,587,928 high-quality clean reads were obtained from RNA-seq, 52,206 unigenes were assembled, and 25,744 unigenes showed significant similarity ( E value < 10 -5 ) to known proteins in the NCBI nonredundant protein database. From those unigenes, we identified 41 candidate OBP proteins, which could be categorized into dimeric OBPs subfamily, minus-C OBPs subfamily, and classical OBPs subfamily. Phylogenic analysis indicated that most maize weevil OBPs were closely related to their orthologues in other beetles of the Superfamily Curculionoidea. We further investigated the expression profiles of those candidate OBP genes by quantitative real-time polymerase chain reaction. Twenty-six of forty-one maize weevil OBP genes were highly expressed in the antennae or other parts of the head. The rest were expressed in the legs, wings, or other tested tissues. The antennal transcriptomic data and candidate OBP genes described here provide a basis for the functional studies of the maize weevil chemical perception, which are potential novel targets for pest control strategies., (© 2019 Wiley Periodicals, Inc.)

Cancer harbors variable heterogeneity and plasticity. Thus far, our comprehension is greatly based on cell lines, organoids, and patient-derived tumor xenografts (PDTXs). Organoids are a three-dimensional in vitro culture platform constructed from self-organizing stem cells. They can almost accurately recapitulate tumor heterogeneity and microenvironment "in a dish," which surpass established cell lines and are not as expensive and time-consuming as PDTXs. As an intermediate model, tumor organoids are also used to study the fundamental issues of tumorigenesis and metastasis. They are specifically applied for drug testing and stored as "living biobanks." In this review, we highlight the translational applications of organoid technologies in tumor research and precision medicine, discuss the advantages and limitations compared with other mentioned methods, and provide our outlook on its future., (Copyright © 2017 Elsevier B.V. All rights reserved.)

S-doped Na2Ti6O13@TiO2 (S-TTO) core-shell nanorods, with exposed anatase TiO2 {101} facets, were synthesized by a facile calcination method. It was found that the addition of thiourea as the sulfur precursor was beneficial for the formation of anatase TiO2 with a better crystallinity and the doped sulfur atoms favorably stabilized the anatase structure. The substitution of Ti(4+) by S(6+) in the lattice of S-TTO gave rise to the visible light response and increased the amount of active groups typically as a hydroxyl radical adsorbed onto the catalyst surface. With the formation of the Ti-O-S bond, partial electrons could be transferred from S to O atoms. The electron-deficient S atoms might capture e(-) and thus inhibit the recombination of photogenerated electron-hole pairs. Meanwhile, a closely contacted interface was formed between Na2Ti6O13 and anatase TiO2, resulting in a nanoscale heterojunction structure to speed up the separation rate of photogenerated charge carriers. The exposed anatase {101} facets could act as possible reservoirs of the photogenerated electrons, yielding a highly reactive surface for the reduction of O2 to O2˙(-) and thus the decrease of recombination probability of electron-hole pairs. In addition, the anisotropically shaped titanate nanorods provided a pathway for the quick transport of charge carriers throughout the longitudinal direction. The combined effects of S doping, nano-heterojunction formation and morphology engineering led to an obviously enhanced photocatalytic performance for the degradation of methylene blue (MB) solution under visible light irradiation. The corresponding photocatalytic mechanism was investigated and discussed in detail. The present work may provide an insight into the fabrication of delicate composite photocatalysts with excellent performance.

This study investigated the effects of Ureaplasma urealyticum infection and raised seminal leucocyte levels on sperm morphology in 967 infertile males and 201 fertile healthy volunteers. U. urealyticum infection led to a significant decrease in the percentage of morphologically normal sperm in infertile males. There was a clear correlation between U. urealyticum infection, raised seminal leucocytes and abnormal sperm morphology. The percentage of morphologically normal sperm was significantly lower in U. urealyticum-positive than U. urealyticum-negative infertile males or fertile controls. The percentage of morphologically normal sperm was lowest in U. urealyticum-positive males with raised seminal leucocytes. Previous studies have found raised seminal leucocyte levels to be associated with reactive oxygen species. The authors suggest that oxidative stress contributes to the effects of U. urealyticum on sperm morphology. In conclusion, U. urealyticum infection can negatively affect sperm morphology and this study provided two possible mechanistic explanations.

Purposes: To investigate the frequencies of AZF microdeletions and chromosomal abnormalities in infertile men from Northeastern China. Moreover, to compare the prevalence of these abnormalities with other countries and regions in the world., Methods: 305 infertile men were enrolled. A complete semen analysis and reproductive hormones were measured according to standard methods. Multiplex polymerase chain reaction (PCR) amplification using nine specific sequence-tagged sites (STS) were used to detect AZF microdeletions. Karyotype analyses were performed on peripheral blood lymphocytes with standard G-banding., Results: Of the 305 infertile men, 28 (9.2%) had AZF microdeletions and 26 (8.5%) had chromosomal abnormalities. The most frequent microdeletions were in the AZFc+d, followed by AZFc, AZFb+c+d and AZFa. A total of 19 patients (82.6%) had Klinefelter's syndrome (47, XXY) in the azoospermic group., Conclusions: The freqencies of AZF microdeletions and chromosomal abnormalities in infertile men from Northeastern China were comparable with infertile men from other countries and regions. However, there was a slightly higher prevalence rate of AZF microdeletions in oligozoospermic patients than reported in previous studies.