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352 results on '"Han, Yingchao"'

4. Programmable DNA hydrogel provides suitable microenvironment for enhancing autophagy-based therapies in intervertebral disc degeneration treatment

Author

Song Qingxin, Jiang Kai, Zheng Dandan, Jin Linyu, Chen Xiuyuan, Feng Yubo, Wang Kun, Han Yingchao, Chen Hao, Song Jie, Chen Zhi, and Shen Hongxing

Subjects
Intervertebral disc degeneration, Gene therapy, DNA hydrogel, Autophagy, Extracellular matrix, Biotechnology, TP248.13-248.65, Medical technology, R855-855.5
Abstract

Abstract The pathogenesis of intervertebral disc degeneration (IVDD) is attributed to metabolic dysregulation within the extracellular matrix and heightened apoptosis of nucleus pulposus cells (NPC). Therefore, a potential therapeutic strategy for managing IVDD involves the reestablishment of metabolic equilibrium within the extracellular matrix and the suppression of excessive myeloid cell apoptosis. The microRNA, miR-5590, displays marked differential expression in degenerative nucleus pulposus (NP) tissues and exerts a direct influence on the regulation of DDX5 expression. This, in turn, modulates mammalian target of rapamycin (mTOR) phosphorylation, thereby impacting autophagy and apoptosis. However, ensuring the smooth delivery of miRNA to a specific injury site poses a significant challenge. To address this issue, a multifunctional DNA hydrogel was developed and subsequently loaded with miR-5590 via spherical nucleic acids (SNAs) for the treatment of IVDD. The hydrogel, which exhibits versatility, has the potential to be administered through injection at the site of injury, resulting in a consistent and prolonged release of miR-5590. This leads to the creation of a genetic microenvironment within the NP, which triggers the onset of autophagy in NPCs and subsequently suppresses apoptosis. As a result, this process regulates the metabolic equilibrium within the extracellular matrix, thereby impeding the in vitro and in vivo progression of IVDD. The amalgamation of miRNAs and biomaterials offers a promising therapeutic strategy for the management of IVDD in clinical settings.

Published
2023
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31. Polyacrylic Acid-Ca2+(Ce3+) Nanoclusters as Fluorescent "Off–On–Off" Probes for Quantitative Detection of Pyrophosphate and Alkaline Phosphatase.

Author

Fan, Yiran, Ding, Ziyou, Chen, Jia, Li, Chengyu, and Han, Yingchao

Abstract

Alkaline phosphatase (ALP) is an essential enzyme widely distributed in the human body, and it can hydrolyze pyrophosphate (PPi) under alkaline conditions. Therefore, ALP and PPi activities are associated with many diseases and it is crucial to develop sensitive and selective methods for detecting ALP and PPi. Herein, poly-(acrylic acid) (PAA)-Ca2+(Ce3+) nanoclusters fluorescent probe was synthesized aiming to detect PPi and ALP quantitatively. The nanoclusters present monodisperse, and their particle size is 0.9–2.4 nm. The probe exhibits no fluorescence due to electron transfer; whereas it can combine with PPi to yield fluorescence increase, arising from the enhanced electric-dipole transition of Ce3+ under the ligand effect of PPi. Moreover, the probe displays good selectivity to PPi, and the fluorescence changes display a linear relationship with PPi concentration of 3–20 mM (y = 11.29x – 23.47, R2 = 0.994). Furthermore, the probe was mixed with PPi to form PAA-Ca-(Ce):PPi nanoparticle (Monodisperse, average size: 1.94 nm) and showed high fluorescence selectivity and responsiveness to ALP due to the ALP hydrolyzing effect and the fluorescence difference between PAA-Ca-(Ce):PPi and PAA-Ca-(Ce):Pi. Based on the relationship between the fluorescence reduction rate of the probe and the logarithm of ALP concentration (y = 0.0594x – 0.00111, 5–200 U/L, R2 = 0.996), ALP was successfully detected quantitatively, and the detection limit is calculated to be 0.62 U/L. Through this approach, we developed a fluorescence "turn-on" for PPi detection and "turn-off" for ALP detection. This study provides a promising strategy for the diagnosis of diseases related to PPi and ALP. [ABSTRACT FROM AUTHOR]

Published
2024
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