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The vision system provides an important way for construction robots to obtain the type and spatial location information of the object. The characteristics of the construction environment, construction object, and robot structure are jointly examined in this paper to propose an approach of object segmentation by spraying the robot based on multi-layer perceptron. Firstly, the hand-eye system experimental platform is built through establishing the mathematical model of the system and calibrating the parameters of the model. Secondly, effort is made to carry out research on image preprocessing algorithms and related experiments, and compare the effects of different binocular stereo-matching algorithms in the actual engineering environment. Finally, research and an experiment are conducted to identify the applicability and effect of the depth image object segmentation algorithm based on multi-layer perceptron. The experimental results prove that the application of multi-layer perceptron to object segmentation by spraying robots can meet the requirement on solution accuracy and is suitable for the object segmentation of complex projects in real life. This approach not only overcomes the shortcomings of the existing recognition methods that are poor in accuracy and difficult to be used widely, but also provides basic data for the subsequent three-dimensional reconstruction, thus making a significant contribution to the research of image processing by spraying robots. [ABSTRACT FROM AUTHOR]