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818 results on '"Han, Jongyoon"'

6. Additional contributions from: Nobel Symposium 162 - Microfluidics

Author

Löfås, Stefan, Herr, Amy E., Qin, Jianhua, Knowles, Tuomas, Kitamori, Takehiko, Lu, Hang, Beebe, David J., Han, Jongyoon, Landers, James, Manz, Andreas, Zengerle, Roland, Weitz, David A., Elf, Johan, and Laurell, Thomas

Subjects
Physics - Fluid Dynamics, Quantitative Biology - Other Quantitative Biology
Abstract

Series of short contributions that are part of Nobel Symposium 162 - Microfluidics arXiv:1712.08369., Comment: Nobel Symposium 162, Stockholm, Sweden, 2017 arXiv:1712.08369v1 Report-no: Nobel162/2017/00

Published
2018

7. Theoretical Limits and Scaling Laws for Electrokinetic Molecular Concentration via Ion Concentration Polarization

Author

Ouyang, Wei, Li, Zirui, Ye, Xinghui, and Han, Jongyoon

Subjects
Physics - Fluid Dynamics, Condensed Matter - Soft Condensed Matter
Abstract

We develop the first theoretical model for the analytical description of ion concentration polarization (ICP)-based electrokinetic molecular concentration, which had not been possible due to the extraordinary complexity of the system. We define the two separate limits for the enrichment factor achievable in a given system and derive the scaling laws for critical parameters, which are validated by numerical simulations and experiments. This work provides clear theoretical explanations on the diverse experimental behaviors previously observed yet unexplainable, while setting solid foundation for the engineering of ICP-based concentrators and other fluid-coupled electrokinetic systems.

Published
2018

12. Accurate Multi-physics Numerical Analysis of Particle Preconcentration Based on Ion Concentration Polarization

Author

Li, Zirui, Liu, Wei, Zhu, Yudan, Lu, Xiaohua, Gu, Yuantong, and Han, Jongyoon

Subjects
Physics - Fluid Dynamics
Abstract

This paper studies mechanism of preconcentration of charged particles in a straight micro-channel embedded with permselective membranes, by numerically solving coupled transport equations of ions, charged particles and solvent fluid without any simplifying assumptions. It is demonstrated that trapping and preconcentration of charged particles are determined by the interplay between drag force from the electroosmotic fluid flow and the electrophoretic force applied trough the electric field. Several insightful characteristics are revealed, including the diverse dynamics of co-ions and counter ions, replacement of co-ions by focused particles, lowered ion concentrations in particle enriched zone, and enhanced electroosmotic pumping effect etc. Conditions for particles that may be concentrated are identified in terms of charges, sizes and electrophoretic mobilities of particles and co-ions. Dependences of enrichment factor on cross-membrane voltage, initial particle concentration and buffer ion concentrations are analyzed and the underlying reasons are elaborated. Finally, post priori a condition for validity of decoupled simulation model is given based on charges carried by focused charge particles and that by buffer co-ions. These results provide important guidance in the design and optimization of nanofluidic preconcentration and other related devices., Comment: 18 pages, 11 firgures

Published
2017
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35. Scalable Mesenchymal Stem Cells Enrichment from Bone Marrow Aspirate using Deterministic Lateral Displacement (DLD) Microfluidics Sorting

Author

Tan, Nicholas, primary, Kwek, Kerwin Zeming, additional, Teo, Kim Leng, additional, Loberas, Mavis, additional, Lee, Jia Ling, additional, Goh, Chin Ren, additional, Yang, Dahou, additional, Oh, Steve, additional, Hui, James, additional, Cool, Simon, additional, Wei Hou, Han, additional, and Han, Jongyoon, additional

Published
2023
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39. Continuous-flow macromolecular sieving in slanted nanofilter array: stochastic model and coupling effect of electrostatic and steric hindrance.

Author

Ko, Sung Hee, Park, Pyeong Jun, and Han, Jongyoon

Subjects
STERIC hindrance, STOCHASTIC models, SIEVES, ONLINE monitoring systems, ELECTRIC fields, MACROMOLECULES
Abstract

Microfabricated slanted nanofilter arrays are a promising technology for integrated biomolecule analysis systems such as online monitoring and point-of-care quality validation, due to their continuous-flow and one-step operation capability. However, an incomplete understanding of the system limits the performance and wider applications of slanted nanofilter arrays. In this paper, we present rigorous theoretical and experimental studies on macromolecule sieving in a slanted nanofilter array. From both stochastic and kinetic models, an explicit theoretical solution describing size-dependent molecule sieving was derived, which was validated using experimental sieving results obtained for various sieving conditions. Our results not only detail the relationship between sieving conditions and sieving efficiency but also demonstrate that sieving is affected by multiple hindrance effects (electrostatic hindrance), not steric hindrance alone. There is an optimal sieving condition for achieving the greatest separation efficiency for DNAs of a certain size range. Small DNA has great size selectivity in small nanofilters and in weak electric fields, whereas large DNA is present in large nanofilters and in strong electric fields. This study provides insights into designing a slanted nanofilter array for particular target applications and understanding the sieving principles in the nanofilter array. [ABSTRACT FROM AUTHOR]

Published
2023
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40. Scalable mesenchymal stem cell enrichment from bone marrow aspirate using deterministic lateral displacement (DLD) microfluidic sorting.

Author

Tan Kwan Zen, Nicholas, Zeming, Kerwin Kwek, Teo, Kim Leng, Loberas, Mavis, Lee, Jialing, Goh, Chin Ren, Yang, Da Hou, Oh, Steve, Hui Hoi Po, James, Cool, Simon M., Hou, Han Wei, and Han, Jongyoon

Subjects
BONE marrow, BLOOD cells, MESENCHYMAL stem cells, STEM cell treatment, REGENERATIVE medicine, HARVESTING, CENTRIFUGATION
Abstract

The growing interest in regenerative medicine has opened new avenues for novel cell therapies using stem cells. Bone marrow aspirate (BMA) is an important source of stromal mesenchymal stem cells (MSCs). Conventional MSC harvesting from BMA relies on archaic centrifugation methods, often leading to poor yield due to osmotic stress, high centrifugation force, convoluted workflow, and long experimental time (∼2–3 hours). To address these issues, we have developed a scalable microfluidic technology based on deterministic lateral displacement (DLD) for MSC isolation. This passive, label-free cell sorting method capitalizes on the morphological differences between MSCs and blood cells (platelets and RBCs) for effective separation using an inverted L-shaped pillar array. To improve throughput, we developed a novel multi-chip DLD system that can process 2.5 mL of raw BMA in 20 ± 5 minutes, achieving a 2-fold increase in MSC recovery compared to centrifugation methods. Taken together, we envision that the developed DLD platform will enable fast and efficient isolation of MSCs from BMA for effective downstream cell therapy in clinical settings. [ABSTRACT FROM AUTHOR]

Published
2023
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48. Ultra-High Throughput Enrichment of Viable Circulating Tumor Cells

Author

Khoo, B. L., Warkiani, M. E., Guan, Guofeng, Tan, Daniel Shao-Weng, Lim, Alvin S. T., Lim, Wan-Teck, Yap, Yoon Sim, Lee, Soo Chin, Soo, Ross A., Han, Jongyoon, Lim, Chwee Teck, MAGJAREVIC, Ratko, Editor-in-chief, Ładyzynsk, Piotr, Series editor, Ibrahim, Fatimah, Series editor, Lackovic, Igor, Series editor, Rock, Emilio Sacristan, Series editor, and Goh, James, editor

Published
2014
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