Search

Your search keyword '"Han, Huanan"' showing total 8 results
Start Over Author "Han, Huanan"
8 results on '"Han, Huanan"'

4. Arabidopsis SRPKII family proteins regulate flowering via phosphorylation of SR proteins and effects on gene expression and alternative splicing

Author

Wang, Tongtong, primary, Wang, Xiaofeng, additional, Wang, Haiyan, additional, Yu, Chao, additional, Xiao, Chengyun, additional, Zhao, Yiwu, additional, Han, Huanan, additional, Zhao, Shuangshuang, additional, Shao, Qun, additional, Zhu, Jianhua, additional, Zhao, Yanxiu, additional, Wang, Pingping, additional, and Ma, Changle, additional

Published
2023
Full Text
View/download PDF

6. ArabidopsisSRPKII family proteins regulate flowering via phosphorylation of SR proteins and effects on gene expression and alternative splicing.

Author

Wang, Tongtong, Wang, Xiaofeng, Wang, Haiyan, Yu, Chao, Xiao, Chengyun, Zhao, Yiwu, Han, Huanan, Zhao, Shuangshuang, Shao, Qun, Zhu, Jianhua, Zhao, Yanxiu, Wang, Pingping, and Ma, Changle

Subjects
ALTERNATIVE RNA splicing, RNA splicing, GENE expression, RNA-binding proteins, PROTEIN expression, SHOOT apexes, SPLICEOSOMES, INTRONS
Abstract

Summary: Alternative splicing of pre‐mRNAs is crucial for plant growth and development. Serine/arginine‐rich (SR) proteins are a conserved family of RNA‐binding proteins that are critical for both constitutive and alternative splicing. However, how phosphorylation of SR proteins regulates gene transcription and alternative splicing during plant development is poorly understood.We found that the Arabidopsis thaliana L. SR protein‐specific kinase II family proteins (SRPKIIs) play an important role in plant development, including flowering. SRPKIIs regulate the phosphorylation status of a subset of specific SR proteins, including SR45 and SC35, which subsequently mediates their subcellular localization.A phospho‐dead SR45 mutant inhibits the assembly of the apoptosis‐and splicing‐associated protein complex and thereby upregulates the expression of FLOWERING LOCUS C (FLC) via epigenetic modification. The splicing efficiency of FLC introns was significantly increased in the shoot apex of the srpkii mutant. Transcriptomic analysis revealed that SRPKIIs regulate the alternative splicing of c. 400 genes, which largely overlap with those regulated by SR45 and SC35‐SCL family proteins.In summary, we found that Arabidopsis SRPKIIs specifically affect the phosphorylation status of a subset SR proteins and regulate the expression and alternative splicing of FLC to control flowering time. [ABSTRACT FROM AUTHOR]

Published
2023
Full Text
View/download PDF

8. Generation of a high-efficiency adenine base editor with TadA8e for developing wheat dinitroaniline-resistant germplasm

Author

Han, Huanan, Wu, Ziwen, Zheng, Ling, Han, Jingyi, Zhang, Yi, Li, Jihu, Zhang, Shujuan, Li, Genying, Ma, Changle, and Wang, Pingping

Abstract

Base editing using CRISPR technologies is an invaluable tool for crop breeding. One of the major base editors, the adenine base editor (ABE), has been successfully used in both model plants and many crops. However, owing to limited editing efficiency, the ABE has been difficult to apply in polyploid crops such as allohexaploid bread wheat that often require simultaneous mutation of multiple alleles for fast breeding. We have designed a wheat high-efficiency ABE (WhieABE), using the newly developed high-activity adenosine deaminase TadA8e. In vivoand in vitroanalysis demonstrated the improved applicability of TadA8e over the commonly used TadA7.10. Dinitroaniline is a widely used herbicide with high effectiveness and low toxicity to animals. However, wheat cultivars with tolerance to dinitroaniline are rare, limiting the application of dinitroaniline in wheat planting. Using A-to-G editing with WhieABE, we found that a Met-to-Thr mutation in wheat tubulin alleles located on chromosomes 1A, 1B, 1D, 4A, and 4D increased the resistance of wheat to dinitroaniline, revealing a dosage effect of edited tubulins in resistance. The WhieABE promises to be a valuable editing tool for accelerating crop improvement and developing herbicide-resistant wheat germplasm.

Published
2021
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results