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1. Calculated Nationalism in Contemporary South Korea

Author

Han, Gil-Soo

Subjects
Grassroots Nationalism, Candlelight Revolution, Political and Social Movements, Critical Realism, thema EDItEUR::J Society and Social Sciences::JP Politics and government, thema EDItEUR::J Society and Social Sciences::JB Society and culture: general
Abstract

Nationalism in a nation-state reflects its emergent structural, cultural, and personal properties at a given time. In the politico-historical context of South Korea and the globe, the fruits of the 1968 Revolution in France could not reach Korean society under its military regime and exploitative economic structure. This continued to frustrate the grassroots and especially social actors in South Korea, which eventually brought about the June Struggle in 1987 and the 2016–2017 Candlelight Revolution. Calculated Nationalism in Contemporary South Korea sketches Korean grassroots’ perception of their nation-state, national identities, and what they desire regarding the future direction of their nation-state. The grassroots have openly spoken out about their frustrations through political rallies and media. This book attempts to reflect the minds of Korean progressives regarding, in particular, the forcibly recruited Japanese military “comfort women,” Abe’s trade provocation against South Korea in 2019, reunification, the 2016–2017 Candlelight Revolution, National Flag-carriers’ struggles, and bullying at work.

Published
2023
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8. Calculated Nationalism in Contemporary Soüth Korea : Movements for Political and Economic Democratization in the 21st Century

Author

Han, Gil-Soo

Subjects
Social Science, Political Science / World / Asian, Political Science
Abstract

Nationalism in a nation-state reflects its emergent structural, cultural, and personal properties at a given time. In the politico-historical context of South Korea and the globe, the fruits of the 1968 Revolution in France could not reach Korean society under its military regime and exploitative economic structure. This continued to frustrate the grassroots and especially social actors in South Korea, which eventually brought about the June Struggle in 1987 and the 2016–2017 Candlelight Revolution. Calculated Nationalism in Contemporary South Korea sketches Korean grassroots’ perception of their nation-state, national identities, and what they desire regarding the future direction of their nation-state. The grassroots have openly spoken out about their frustrations through political rallies and media. This book attempts to reflect the minds of Korean progressives regarding, in particular, the forcibly recruited Japanese military “comfort women,” Abe’s trade provocation against South Korea in 2019, reunification, the 2016–2017 Candlelight Revolution, National Flag-carriers’ struggles, and bullying at work.

Published
2023

27. Introduction

Author

Budarick, John, Han, Gil-Soo, Budarick, John, editor, and Han, Gil-Soo, editor

Published
2017
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37. Expression of Human CTP Synthetase in Saccharomyces cerevisiae Reveals Phosphorylation by Protein Kinase A*

Author

Han, Gil-Soo, Sreenivas, Avula, Choi, Mal-Gi, Chang, Yu-Fang, Martin, Shelley S, Baldwin, Enoch P, and Carman, George M

Subjects
Biochemistry and Cell Biology, Biological Sciences, Genetics, Generic health relevance, Carbon-Nitrogen Ligases, Catalysis, Cyclic AMP-Dependent Protein Kinases, DNA, Dose-Response Relationship, Drug, Electrophoresis, Polyacrylamide Gel, Escherichia coli, Genetic Complementation Test, Humans, Immunoblotting, Models, Chemical, Mutation, Peptides, Phenotype, Phosphoamino Acids, Phospholipids, Phosphorylation, Plasmids, Polymerase Chain Reaction, Saccharomyces cerevisiae, Time Factors, Chemical Sciences, Medical and Health Sciences, Biochemistry & Molecular Biology, Biological sciences, Biomedical and clinical sciences, Chemical sciences
Abstract

CTP synthetase (EC 6.3.4.2, UTP:ammonia ligase (ADP-forming)) is an essential enzyme in all organisms; it generates the CTP required for the synthesis of nucleic acids and membrane phospholipids. In this work we showed that the human CTP synthetase genes, CTPS1 and CTPS2, were functional in Saccharomyces cerevisiae and complemented the lethal phenotype of the ura7Delta ura8Delta mutant lacking CTP synthetase activity. The expression of the CTPS1- and CTPS2-encoded human CTP synthetase enzymes in the ura7Delta ura8Delta mutant was shown by immunoblot analysis of CTP synthetase proteins, the measurement of CTP synthetase activity, and the synthesis of CTP in vivo. Phosphoamino acid and phosphopeptide mapping analyses of human CTP synthetase 1 isolated from (32)P(i)-labeled cells revealed that the enzyme was phosphorylated on multiple serine residues in vivo. Activation of protein kinase A activity in yeast resulted in transient increases (2-fold) in the phosphorylation of human CTP synthetase 1 and the cellular level of CTP. Human CTP synthetase 1 was also phosphorylated by mammalian protein kinase A in vitro. Using human CTP synthetase 1 purified from Escherichia coli as a substrate, protein kinase A activity was dose- and time-dependent, and dependent on the concentrations of CTP synthetase 1 and ATP. These studies showed that S. cerevisiae was useful for the analysis of human CTP synthetase phosphorylation.

Published
2005

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