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The aim of this study was to evaluate, for the first time, the antidiabetic effect of the alkaloids extract of Salvia chudaei Batt. & Trab. (Lamiaceae) on alloxan-induced diabetic rats. The alkaloids extract was prepared, and the in vitro inhibitory effect of key digesting enzymes related to postprandial hyperglycemia were determined. After acute toxicity test, the Swiss albino rats were induced with alloxan to get experimental diabetes animals. The fasting mean blood glucose, lipid profile, different liver and kidney function biomarkers and antioxidant biomarkers levels, after treatment for 30 days, diabetic untreated and diabetic rats treated with alkaloids extract were estimated. The alkaloids displayed remarkable in inhibiting α-glucosidase (IC50 = 248.25±2.61 μg/ml) than α-amylase (IC50 = 262.96±9.64 μg/ml) activities. In vivo, the results proved that alkaloids extract at dose of 500 mg/kg bw decreased significantly the blood glucose, lipid profile levels and improved the liver and kidney function biomarkers and increased the activities of antioxidant enzymes (superoxide dismutase and gluthatione reductase). This study demonstrates, that alkaloids are effective in inhibiting hyperglycemia and oxidative stress caused by diabetes.

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Leuconostoc (Ln) sp. belongs to a group of lactic acid bacteria, which has the capacity to produce dextran (an exopolysaccharides) in the presence of sucrose. dextran is industrially important, it was the first microbial exopolysaccharide affirmed for commercial use. This study aimed to optimize the production of the synthesized dextran by Ln strains species isolated from different dairy products. Morphological, cultural, physiological and biochemical characteristics were employed to identify 23 isolated strains. We have identified the species: Ln. gelidum, Ln. carnosum, Ln. citreum, Ln. fallax, Ln. mesenteroides subsp mesenteroides, Ln. mesenteroides subsp dextranicum, Ln. mesenteroides subsp cremoris. 20 strains had the capacity to produce dextran from sucrose. The precipitation and quantification of EPS on MRSs (Mark rogosa et sharpe sucrose) medium revealed a difference between the strains, by the total sugars assay method, the amount of EPS varied between 0.63 ± 0.19 and 2.41 ± 0.17 g / L of strains LnF70 and LnC1 (isolated from goat's milk), respectively. The dextran production from MRSs medium was better than from liquid MSE. The optimization of production on MRSs medium with different concentration of glucose, yeast extract and sucrose showed that the strains had good production with a concentration of 2% glucose, 0.3% yeast extract and 10% sucrose. [ABSTRACT FROM AUTHOR]