Search

Traditionally, firearm and toolmark examiners manually evaluate the similarity of features on two bullets using comparison microscopy. Advances in microscopy have made it possible to collect 3D topographic data, and several automated comparison algorithms have been introduced for the comparison of bullet striae using these data. In this study, open-source approaches for cross-correlation, congruent matching profile segments, consecutive matching striations, and a random forest model were evaluated. A statistical characterization of these automated approaches was performed using four datasets of consecutively manufactured firearms to provide a challenging comparison scenario. Each automated approach was applied to all samples in a pairwise fashion, and classification performance was compared. Based on these findings, a Bayesian network was empirically learned and constructed to leverage the strengths of each individual approach, model the relationship between the automated results, and combine them into a posterior probability for the given comparison. The network was evaluated similarly to the automated approaches, and the results were compared. The developed Bayesian network classified 99.6% of the samples correctly, and the resultant probability distributions were significantly separated more so than the automated approaches when used in isolation., (© 2024 American Academy of Forensic Sciences.)

Cellular metabolism comprises a complex network of biochemical anabolic and catabolic processes that fuel the growth and survival of living organisms. The enzyme malate dehydrogenase (MDH) is most known for its role in oxidizing malate to oxaloacetate (OAA) in the last step of the tricarboxylic acid (TCA) cycle, but it also participates in the malate-aspartate shuttle in the mitochondria as well as the glyoxylate cycle in plants. These pathways and the specific reactions within them are dynamic and must be carefully calibrated to ensure a balance between nutrient/energy supply and demand. MDH structural and functional complexity requires a variety of regulatory mechanisms, including allosteric regulation, feedback, and competitive inhibition, which are often dependent on whether the enzyme is catalyzing its forward or reverse reaction. Given the role of MDH in central metabolism and its potential as a target for therapeutics in both cancer and infectious diseases, there is a need to better understand its regulation. The involvement of MDH in multiple pathways makes it challenging to identify which effectors are critical to its activity. Many of the in vitro experiments examining MDH regulation were done decades ago, and though allosteric sites have been proposed, none to date have been specifically mapped. This review aims to provide an overview of the current knowledge surrounding MDH regulation by its substrate, products, and other intermediates of the TCA cycle while highlighting all the gaps in our understanding of its regulatory mechanisms., (© 2024 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society.)

There is abundant research showing the disproportionate impacts of violence on health in disadvantaged neighborhoods, making an understanding of recent violent crime trends essential for promoting health equity. Carjackings have been of particular interest in the media, although little research has been undertaken on this violent crime. We use interrupted time series models to examine the impact of the police killing of George Floyd on the spatiotemporal patterns of carjacking in Minneapolis in relation to neighborhood disadvantage. To provide grounding, we compare our results to the well-studied patterns of homicides. Results indicate that carjackings both increased and dispersed spatially after the murder of George Floyd and subsequent social unrest, more so than homicides. Socially disadvantaged neighborhoods experienced the greatest absolute increase while more advantaged neighborhoods saw a greater relative increase. The challenge ahead is to identify policy responses that will effectively curb such violence without resorting to harsh and inequitable policing and sentencing practices., Competing Interests: Declaration of competing interest None., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

The high-profile police murder of George Floyd is likely to have an aftermath of negative health consequences, particularly among Black people. Our study evaluates the impact of the murder of Mr. Floyd on mental health in Black, Latine, and white communities in Minneapolis, Minnesota. We constructed a panel dataset merging data from the Minnesota Hospital Association, Minnesota Department of Natural Resources, Minneapolis Police Department, and American Community Survey. First, we specify an overall and racial subgroup autoregressive interrupted time-series design to identify the impact of the murder on rates of mental health hospital discharge at the city-level. We then examine the spatial heterogeneity in the impact of the murder by specifying zip code tabulation area (ZCTA)-level panel models. We find a 0.23 per 1,000 increase in mental health conditions among Black people in the immediate post-murder period, followed by a weekly decline (-.007) in mental health diagnoses. We do not find a substantial rate increase in White or Latine residents. Further, our analyses at the ZCTA-week-level corroborate these findings, while showing that the increase for Black residents was global. These findings speak to the traumatizing effects of police violence and the short- and longer-term public health consequences for Black communities., (© The Author(s) 2024. Published by Oxford University Press on behalf of the Johns Hopkins Bloomberg School of Public Health. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)

Religious fundamentalism, characterized by rigid adherence to a set of beliefs putatively revealing inerrant truths, is ubiquitous across cultures and has a global impact on society. Understanding the psychological and neurobiological processes producing religious fundamentalism may inform a variety of scientific, sociological, and cultural questions. Research indicates that brain damage can alter religious fundamentalism. However, the precise brain regions involved with these changes remain unknown. Here, we analyzed brain lesions associated with varying levels of religious fundamentalism in two large datasets from independent laboratories. Lesions associated with greater fundamentalism were connected to a specific brain network with nodes in the right orbitofrontal, dorsolateral prefrontal, and inferior parietal lobe. This fundamentalism network was strongly right hemisphere lateralized and highly reproducible across the independent datasets ( r = 0.82) with cross-validations between datasets. To explore the relationship of this network to lesions previously studied by our group, we tested for similarities to twenty-one lesion-associated conditions. Lesions associated with confabulation and criminal behavior showed a similar connectivity pattern as lesions associated with greater fundamentalism. Moreover, lesions associated with poststroke pain showed a similar connectivity pattern as lesions associated with lower fundamentalism. These findings are consistent with the current understanding of hemispheric specializations for reasoning and lend insight into previously observed epidemiological associations with fundamentalism, such as cognitive rigidity and outgroup hostility., Competing Interests: Competing interests statement:The authors declare no competing interest.

We describe a full account of our synthetic strategy leading to the first total synthesis of the manzamine alkaloid lissodendoric acid A . These efforts demonstrate that strained cyclic allenes are valuable synthetic building blocks and can be employed efficiently in total synthesis., (© 2024 Wiley-VCH GmbH.)

This in-depth review delves into the multifaceted realm of mass shootings and explores their epidemiology from a psychological perspective. The article presents a comprehensive examination of the prevalence, perpetrator and victim profiles, motives, and contributing factors associated with mass shootings. By investigating the intricate relationship between masculinity, domestic violence, military service, social media, fame-seeking, suicidal ideation, mental illness, and firearms, this article sheds light on the multifaceted nature of mass shootings. Moreover, it discusses the importance of implementing effective prevention strategies to address this growing public health concern. The findings from this review serve as a valuable resource for researchers, practitioners, policy makers, and the community at large, facilitating a deeper understanding of mass shootings and fostering the development of evidence-based solutions to prevent these tragic incidents.

The Caribbean & Mesoamerica Biogeochemical Isotope Overview (CAMBIO) is an archaeological data community designed to integrate published biogeochemical data from the Caribbean, Mesoamerica, and southern Central America to address questions about dynamic interactions among humans, animals, and the environment in the region over the past 10,000 years. Here we present the CAMBIO human dataset, which consists of more than 16,000 isotopic measurements from human skeletal tissue samples (δ 13 C, δ 15 N, δ 34 S, δ 18 O, 87 Sr/ 86 Sr, 206/204 Pb, 207/204 Pb, 208/204 Pb, 207/206 Pb) from 290 archaeological sites dating between 7000 BC to modern times. The open-access dataset also includes detailed chronological, contextual, and laboratory/sample preparation information for each measurement. The collated data are deposited on the open-access CAMBIO data community via the Pandora Initiative data platform ( https://pandoradata.earth/organization/cambio )., (© 2024. The Author(s).)

Emergent-based practices of leadership development (such as intentional emergence (IE), case-in-point, or group relations) rely a great deal on stopping the action in order to publicly notice group behaviors and patterns and connect what is happening authentically to conscious actions and ideas (such as course content, readings, theories, etc.). However, when a facilitator or participant practices stopping the action and calling out these behaviors, there is a danger that they will go beyond productive tension into a level that causes casualties. This article explores the foundational need for compassion and purpose when using the common tools of heat and noticing in intentionally emergent spaces., (© 2024 The Authors. New Directions for Student Leadership published by Wiley Periodicals LLC.)

While emergent pedagogies offer potentially high impact, the risks in using such pedagogies can be significant when not handled carefully. This article explores the cautions and limitations of emergent-based pedagogies such as case-in-point, intentional emergence, and group relations. Leadership educators who use emergent-based approaches need to be well-versed in how to hold the heat in the classroom, even when the heat may be directed at them. The learning and impact from the pedagogy may sometimes take months or even years to be felt. As such, considerations like administration buy-in are necessary in addition to the adequate development of the facilitator. This article explores resources to develop educators in their use of emergent pedagogies, including hallmark readings and trainings that are available to those interested in learning more., (© 2024 Wiley Periodicals, LLC.)

In a post-pandemic context, the need for leadership students to navigate ambiguous conditions and examine their automatic responses to authority has increased. Yet, common approaches to teaching leadership, such as group discussions and simulations, overlook the potential for using development spaces as living laboratories. This article explores three emergent-based pedagogies (group relations, case-in-point, and intentional emergence) that de-center the instructor, prioritize co-creation and emergence, and provide living laboratories for students to examine their assumptions and default behaviors related to leadership., (© 2024 Wiley Periodicals, LLC.)

Leadership development, like other professional areas such as medicine, teaching, and law, requires students to become as adept at practicing leadership as they are at understanding the theory behind it. For example, K-12 teachers have student teaching, medical students have residencies, cadavers, and virtual reality-but where is the learning laboratory for leadership? The intentional emergence (IE) pedagogy provides a framework for instructors and learners that both honors the role of the instructor as a goal/outcome-setter while also embracing the complexities of the leadership development space as a living laboratory and practice field for leadership. Additionally, IE attempts to bring unconscious and default behaviors to the foreground so that critical components of leadership development such as power, privilege, and identities can be seen and included., (© 2024 Wiley Periodicals, LLC.)

Objective: Predicting which young people are likely to use tobacco in the future is critical for prevention and intervention. Although measures for assessing susceptibility to using tobacco have fulfilled this goal for decades, there is almost no standard for the number of items that should be administered, or which items should be administered for which products. This study explored whether brief but psychometrically sound versions of commonly used susceptibility measures can adequately capture the construct relative to longer measures., Method: A sample of young people ( N = 451; M age = 16.5 years; 64% females; 65% White) completed 33 susceptibility items, which are designed to assess susceptibility to use different types of tobacco products (cigarette, smokeless tobacco, vaping products, and little cigars/cigarillos) of various flavors (tobacco, menthol, and sweet)., Results: Analysis of these 33 items indicated that asking about the likelihood of using each tobacco product class when a best friend offers it (four items in all) captures 98.5% of information that is captured using the longer set of items; asking the best friend question for each product by each flavor category (11 items in all) captures 99.7% of the information., Conclusions: Depending on research needs, tobacco use susceptibility can be measured with little loss of information by administering a limited set of items assessing the likelihood that a young person will use a tobacco product if a friend offers it for any product-flavor combination. (PsycInfo Database Record (c) 2024 APA, all rights reserved).

Swarming locusts present a quintessential example of animal collective motion. Juvenile locusts march and hop across the ground in coordinated groups called hopper bands. Composed of up to millions of insects, hopper bands exhibit aligned motion and various collective structures. These groups are well-documented in the field, but the individual insects themselves are typically studied in much smaller groups in laboratory experiments. We present, to our knowledge, the first trajectory data that detail the movement of individual locusts within a hopper band in a natural setting. Using automated video tracking, we derive our data from footage of four distinct hopper bands of the Australian plague locust, Chortoicetes terminifera . We reconstruct nearly 200 000 individual trajectories composed of over 3.3 million locust positions. We classify these data into three motion states: stationary, walking and hopping. Distributions of relative neighbour positions reveal anisotropies that depend on motion state. Stationary locusts have high-density areas distributed around them apparently at random. Walking locusts have a low-density area in front of them. Hopping locusts have low-density areas in front and behind them. Our results suggest novel insect interactions, namely that locusts change their motion to avoid colliding with neighbours in front of them.

Objective: To determine the impact of the police murder of George Floyd in Minneapolis, MN on firearm violence, and examine the spatial and social heterogeneity of the effect., Methods: We analyzed a uniquely constructed panel dataset of Minneapolis Zip Code Tabulation Areas from 2016-2020 (n = 5742), consisting of Minnesota Hospital Association, Minneapolis Police Department, Minneapolis Public Schools, Census Bureau, and Minnesota Department of Natural Resources data. Interrupted time-series and random effects panel models were used to model the spatiotemporal effects of police killing event on the rate of firearm assault injuries., Results: Findings reveal a rising and falling temporal pattern post-killing and a spatial pattern in which disadvantaged, historically Black communities near earlier sites of protest against police violence experienced the brunt of the post-killing increase in firearm assault injury. These effects remain after adjusting for changes in police activity and pandemic-related restrictions, indicating that rising violence was not a simple byproduct of changes in police behavior or COVID-19 response., Conclusions: The results suggest that the increases in firearm violence as a result of police violence are disproportionately borne by underserved communities., Competing Interests: Declaration of Competing Interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Jeanie Santaularia reports financial support was provided by University of Minnesota Twin Cities and the National Institute for Child Health and Human Development., (Copyright © 2023. Published by Elsevier Ltd.)

Objectives: The role of migration in the cultural development of central Mexico has long been debated. Archaeological models suggest that central Mexico likely experienced increased migration during the Epiclassic period (600-900 CE) and that migrants may have originated in northwestern Mexico. While previous biodistance analyses of Classic and Postclassic populations have come to similar conclusions, none have incorporated Epiclassic skeletal populations. This study uses multi-scalar biodistance analyses to directly evaluate archaeological Epiclassic migration models within central Mexico., Materials and Methods: This study uses finite mixture and relationship (R) matrix analyses of cervicometric tooth dimensions to reconstruct patterns of biological affinity among Classic and Epiclassic Mesoamerican populations (n = 333), including at the central Mexican Epiclassic shrine site of Non-Grid 4 where the remains of at least 180 individuals were interred., Results: Estimated inter-site phenotypic distances demonstrate support for some degree of both biological continuity and extra-local gene flow within central Mexican populations during the Classic-Epiclassic transition. Furthermore, estimated phenotypic distances and finite mixture posterior probabilities indicate central Mexican Epiclassic populations were biologically diverse, originating from various source populations throughout Mesoamerica, including the Bajío region, the Malpaso Valley, and the Oaxaca Valley., Discussion: Results suggest that emphasizing both local and extra-local gene flow rather than population replacement may be more appropriate to understand central Mexican population structure during the Classic-Epiclassic transition. Moreover, analyses support previous archaeological migration models positing that Epiclassic migrants into central Mexico originated in northwestern Mexico, but also find evidence of Epiclassic migrants originating from previously unanticipated locales like southern Mexico., (© 2023 Wiley Periodicals LLC.)

Background: The risk for problematic gambling and associated high-risk behaviors is elevated during adolescence and emerging adulthood. Activities with gambling-like features and novel forms of gambling may place youth at an increased risk for problem gambling., Aim and Method: The aim of this review is to provide an overview of the association between both activities with gambling-like features and novel gambling activities and problem gambling among youth while examining the role of psychopathology and cognitive processes. Six databases (PsychINFO, MEDLINE, PubMed, Social Work Abstracts, Technology Collection, and Scopus) were searched in November 2021 for peer-reviewed articles investigating the association between the aforementioned variables among youth up to the age of 25 years. Risk of bias was assessed using the Observational Study Quality Evaluation., Findings: Forty-five articles were included in the review. Positive associations were observed between engagement in activities with gambling-like features (e.g., video games, social casino games, loot boxes) and problem gambling. Increased involvement with novel forms of gambling (e.g., online sports betting, fantasy sports, and esports betting) were also associated with a greater risk for problematic gambling. Males reported higher rates of engagement in these activities and a greater risk of problem gambling than females. Impulsivity, risk taking, cognitive distortions, and specific emotional vulnerabilities were associated with an increased risk of problem gambling., Conclusions: Despite the need for additional longitudinal research controlling for relevant confounders, these findings underline how engagement in activities with gambling-like features are relevant in the developmental trajectory toward problem gambling., (© 2022 Association for Child and Adolescent Mental Health.)

During the examination of trace evidence, often a realignment along the edges of known and questioned items are made to determine if a physical fit is present and if those objects were once one continuous piece or object. Duct tape is an evidence type in which the evaluation of physical fits is often conducted and is regarded as conclusive evidence of an association between the items. The examination and conclusion of a physical fit between edges relies heavily on examiner discretion to identify distinctive features across the edges since there are no statistical approaches or objective methodologies for the comparison. This study developed an automated image processing and comparison method to quantify tape end matches using cross-correlation scores and an empirical approach to the assessment. Characterization of 150 hand torn duct tape end pair physical fits were also conducted where matching and non-matching sample distributions were created. This study also evaluated partial duct tape edges and the influence this has on a comparison. Given the strength associated with a physical fit and the presence of stretching or deformation along the fractured edge, an understanding of the value these samples have is paramount. Furthermore, random match probabilities were calculated based on the correlation scores from the inter-comparisons to model the weight of evidence or strength of association between the edges. Finally, the study demonstrated that not every true match holds the same association strength through score distributions, but the approach is able to distinguish matching and non-matching samples at edge widths greater than 27 %., (Copyright © 2022 Elsevier B.V. All rights reserved.)

Community-level vulnerability to pyramid scheme fraud may be affected by place-based sources of strain and opportunity. Using national victim data from a pyramid scheme fraud case from 2000-2013, this research explores pyramid scheme adoption with group-based trajectory modeling (GBTM). GBTM is used to look for distinct trajectories of pyramid scheme join rates and to explore the effect of strain, as measured by a county's Social Vulnerability Index and unemployment rate, and opportunity or protection, as measured by a series of social capital variables, on the group trajectories. Findings suggest that county-level strain, including the county's Social Vulnerability Index and unemployment rate are related to pyramid scheme victimization, especially early adoption. We also find that social capital variables - which can, in theory, reduce strain or increase opportunity - have a nuanced relationship with fraud victimization. While our findings are drawn from a single pyramid scheme, they point to the potential to analyze case data to inform preventative and monitoring strategies appropriate to local-level characteristics., (© The Author(s), under exclusive licence to Springer Nature B.V. 2022, Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.)

Metformin is used globally to treat type II diabetes, has demonstrated anti-ageing and COVID mitigation effects and is a major anthropogenic pollutant to be bioremediated by wastewater treatment plants (WWTPs). Metformin is not adsorbed well by activated carbon and toxic N-chloro derivatives can form in chlorinated water. Most earlier studies on metformin biodegradation have used wastewater consortia and details of the genomes, relevant genes, metabolic products, and potential for horizontal gene transfer are lacking. Here, two metformin-biodegrading bacteria from a WWTP were isolated and their biodegradation characterized. Aminobacter sp. MET metabolized metformin stoichiometrically to guanylurea, an intermediate known to accumulate in some environments including WWTPs. Pseudomonas mendocina MET completely metabolized metformin and utilized all the nitrogen atoms for growth. Pseudomonas mendocina MET also metabolized metformin breakdown products sometimes observed in WWTPs: 1-N-methylbiguanide, biguanide, guanylurea, and guanidine. The genome of each bacterium was obtained. Genes involved in the transport of guanylurea in Aminobacter sp. MET were expressed heterologously and shown to serve as an antiporter to expel the toxic guanidinium compound. A novel guanylurea hydrolase enzyme was identified in Pseudomonas mendocina MET, purified, and characterized. The Aminobacter and Pseudomonas each contained one plasmid of 160 kb and 90 kb, respectively. In total, these studies are significant for the bioremediation of a major pollutant in WWTPs today., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2022 Martinez-Vaz, Dodge, Lucero, Stockbridge, Robinson, Tassoulas and Wackett.)

The implementation of course-based undergraduate research experiences (CUREs) has made it possible to expose large undergraduate populations to research experiences. For these research experiences to be authentic, they should reflect the increasingly collaborative nature of research. While some CUREs have expanded, involving multiple schools across the nation, it is still unclear how a structured extramural collaboration between students and faculty from an outside institution affects student outcomes. In this study, we established three cohorts of students: 1) no-CURE, 2) single-institution CURE (CURE), and 3) external collaborative CURE (ec-CURE), and assessed academic and attitudinal outcomes. The ec-CURE differs from a regular CURE in that students work with faculty member from an external institution to refine their hypotheses and discuss their data. The sharing of ideas, data, and materials with an external faculty member allowed students to experience a level of collaboration not typically found in an undergraduate setting. Students in the ec-CURE had the greatest gains in experimental design; self-reported course benefits; scientific skills; and science, technology, engineering, and mathematics (STEM) importance. Importantly this study occurred in a diverse community of STEM disciplinary faculty from 2- and 4-year institutions, illustrating that exposing students to structured external collaboration is both feasible and beneficial to student learning.

From differences in brain development to balancing complex roles, the needs of graduate students significantly differ from undergraduates and yet often go unacknowledged. In this article, I explore how these differences can be taken into account when supporting graduate student leadership identity during turbulent times and beyond through in-person and online leadership development programs., (© 2022 Wiley Periodicals, LLC.)

Premise: Reproductive fitness of individual plants depends on the timing of flowering, especially in mate-limited populations, such as those in fragmented habitats. When flowering time traits are associated with differential reproductive success, the narrow-sense heritability (h 2 ) of traits will determine how rapidly trait means evolve in response to selection. Heritability of flowering time is documented in many annual plants. However, estimating h 2 of flowering time in perennials presents additional methodological challenges, often including paternity assignment and trait expression over multiple years., Methods: We evaluated the h 2 of onset and duration of flowering using offspring-midparent regressions and restricted maximum likelihood methods in an experimental population of an iterocarpic, perennial, herbaceous plant, Echinacea angustifolia, growing in natural conditions. We assessed the flowering time of the parental cohort in 2005 and 2006; the offspring in 2014 through 2017. We also examined the effects of the paternity assignment from Cervus and MasterBayes on estimates of h 2 ., Results: We found substantial h 2 for onset and duration of flowering. We also observed variation in estimates among years. The most reliable estimates for both traits fell in the range of 0.1-0.17. We found evidence of a genotype by year interaction for onset of flowering and strong evidence that genotypes are consistent in their duration of flowering across years., Conclusions: Substantial heritabilities in this population imply the capacity for a response to natural selection, while also suggesting the potential for differential contributions to adaptive evolution among seasons., (© 2022 Botanical Society of America.)

White individuals in the United States (US) have historically had disproportionate access to firearms. The real-life availability of firearms, including those most lethal, may still be greater among White populations, manifesting in the number of victims in shootings. We compared the severity of US mass public shootings since Columbine by race and/or ethnicity of the perpetrator using The Violence Project Database of Mass Shooters, assessing fatalities (minimum four), total victims, type, and legal status of guns used. We used data visualization and Quasi-Poisson regression of victims minus four - accounting for truncation at 4 fatalities - to assess fatality and total victim rates comparing Non-Hispanic (NH) White with NH Black shooters, using winsorization to account for outlier bias from the 2017 Las Vegas shooting. In 104 total mass public shootings until summer 2021, NH White shooters had higher median fatalities (6 [IQR 5-9] versus 5 [IQR 4-6]) and total victims (9 [IQR 6-19] versus 7 [IQR 5-12]) per incident. Confidence intervals of NH Black versus NH White fatalities rate ratios (RR) ranged from 0.17-1.15, and of total victim RRs from 0.15-1.04. White shooters were overrepresented in mass public shootings with the most victims, typically involving legally owned assault rifles. To better understand the consequences when firearms are readily available, including assault rifles, we need a database of all US gun violence. Our assessment of total victims beyond fatalities emphasizes the large number of US gun violence survivors and the need to understand their experiences to capture the full impact of gun violence., (Copyright © 2022 Elsevier Inc. All rights reserved.)

This article presents outcomes from a Workshop entitled "Bioarchaeology: Taking Stock and Moving Forward," which was held at Arizona State University (ASU) on March 6-8, 2020. Funded by the National Science Foundation (NSF), the School of Human Evolution and Social Change (ASU), and the Center for Bioarchaeological Research (CBR, ASU), the Workshop's overall goal was to explore reasons why research proposals submitted by bioarchaeologists, both graduate students and established scholars, fared disproportionately poorly within recent NSF Anthropology Program competitions and to offer advice for increasing success. Therefore, this Workshop comprised 43 international scholars and four advanced graduate students with a history of successful grant acquisition, primarily from the United States. Ultimately, we focused on two related aims: (1) best practices for improving research designs and training and (2) evaluating topics of contemporary significance that reverberate through history and beyond as promising trajectories for bioarchaeological research. Among the former were contextual grounding, research question/hypothesis generation, statistical procedures appropriate for small samples and mixed qualitative/quantitative data, the salience of Bayesian methods, and training program content. Topical foci included ethics, social inequality, identity (including intersectionality), climate change, migration, violence, epidemic disease, adaptability/plasticity, the osteological paradox, and the developmental origins of health and disease. Given the profound changes required globally to address decolonization in the 21st century, this concern also entered many formal and informal discussions., (© 2022 American Association of Biological Anthropologists.)

During vertebrate development, neural crest cells (NCCs) migrate extensively and differentiate into various cell types that contribute to structures like the craniofacial skeleton and the peripheral nervous system. While it is critical to understand NCC migration in the context of a 3D embryo, isolating migratory cells in 2D culture facilitates visualization and functional characterization, complementing embryonic studies. The present protocol demonstrates a method for isolating chick cranial neural folds to generate primary NCC cultures. Migratory NCCs emerge from neural fold explants plated onto a fibronectin-coated substrate. This results in dispersed, adherent NCC populations that can be assessed by staining and quantitative morphological analyses. This simplified culture approach is highly adaptable and can be combined with other techniques. For example, NCC emigration and migratory behaviors can be evaluated by time-lapse imaging or functionally queried by including inhibitors or experimental manipulations of gene expression (e.g., DNA, morpholino, or CRISPR electroporation). Because of its versatility, this method provides a powerful system for investigating cranial NCC development.

Fetal alcohol spectrum disorders (FASD) are neurodevelopmental/neurobehavioral conditions caused by prenatal alcohol exposure (PAE). Impairments caused by PAE contribute to the over-representation of individuals with FASD in the United States juvenile and adult criminal justice systems. These same impairments can equally impact on individuals with FASD who are witnesses to or victims of crime who also have to navigate the complexities of the criminal justice system. Difficulties include increased susceptibility to confabulation throughout the legal process that, in turn, can contribute to increased rates of poor outcomes including false confessions and wrongful convictions. Individuals with FASD are particularity at risk of confabulation when they are subjected to tactics, such as stressful and anxiety-provoking situations, threats, and leading, suggestive, or coercive questioning. Many professionals in the forensic context are unfamiliar with FASD or related confabulation risk and may unintentionally utilize tactics that intensify impacts of pre-existing impairment. This article serves as a beginner's guide for professionals working in criminal justice settings by (a) providing research-based overviews of FASD and confabulation, (b) describing how FASD may lead to confabulation, and (c) suggesting ways that professionals can modify protocols when interacting with individuals with FASD. Suggestions in this article hold the potential to decrease the risk of confabulation in the criminal justice system and decrease problematic outcomes, such as false confessions and wrongful convictions among individuals with FASD., (© 2021 John Wiley & Sons Ltd.)

The objective prevalence of and subjective vulnerability to infectious diseases are associated with greater ingroup preference, conformity, and traditionalism. However, evidence directly testing the link between infectious diseases and political ideology and partisanship is lacking. Across four studies, including a large sample representative of the U.S. population ( N  > 12,000), we demonstrate that higher environmental levels of human transmissible diseases and avoidance of germs from human carriers predict conservative ideological and partisan preferences. During the COVID-19 pandemic ( N  = 848), we replicated this germ aversion finding and determined that these conservative preferences were primarily driven by avoidance of germs from outgroups (foreigners) rather than ingroups (locals). Moreover, socially conservative individuals expressed lower concerns of being susceptible to contracting infectious diseases during the pandemic and worried less about COVID-19. These effects were robust to individual-level and state-level controls. We discuss these findings in light of theory on parasite stress and the behavioral immune system and with regard to the political implications of the COVID-19 pandemic., (© 2021 The Authors. Political Psychology published by Wiley Periodicals LLC on behalf of International Society of Political Psychology.)

Chains of nickel nanoparticles coated with few nitrogen-doped graphitic carbon layers (Ni@NC) are synthesized by hydrogen-assisted pyrolysis of Ni-ZIF. Hydrogen and temperature can play key roles in the formation of oriented Ni@NC nanoparticle chains, and carbon shells can protect Ni nanoparticles from external oxidation and aggregations. Under the optimized potential (0.60 V vs. Ag/AgCl), the Ni@NC7H nanoparticle chains obtained at 700 °C under H 2 /Ar atmosphere (Ni@NC7H) demonstrate outstanding performances, such as high sensitivity of 1.44 mA mM -1  cm -2 (RSD = 1.0%), low detection limit of 0.34 μM (S/N = 3), broad linear range from 1 μM to 1.81 mM, and excellent application potential in artificial sweat and human serum. Therefore, the findings above indicate that this study will provide a general methodology for the synthesis of chains-like core-shell nanoparticle electrocatalysts for non-enzymatic glucose detection., (© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.)

From a forensic perspective, a presumptive test, one which indicates the presence or absence of a certain target material such as blood, is an invaluable tool. Among these tests, there are different specificities, sensitivities, and shelf lives. The accuracy of a test is an algebraic combination of the specificity and sensitivity of the test. Each test has limitations as given by its false positive and false negative rates. The aim of this study was to illustrate how the false positive and false negative rates are to be properly determined using a simulation study for the phenolphthalein test. New presumptive tests must be properly evaluated/validated through testing of commonly encountered household items and other potentially probative items usually found at crime scenes, however, the makeup of test sets must appropriately capture all error rates. In order to correctly use these results when the test is applied to an unknown sample recovered at a crime scene, the error rates cannot be applied directly to estimate whether or not the sample is actually the analyte of interest. In a validation study, the forensic scientist calculates the false positive rate as the p(Positive Reaction|Blood), whereas at the scene, the crime scene investigator wishes to determine the p(Blood|Positive Reaction). All crime scene investigators need to ensure that the conditional is not transposed when interpreting such results. Furthermore, this work provides a model for the assessment of a multiple test diagnostic system intended for investigators., (© 2021 American Academy of Forensic Sciences.)

Importance: Understanding the motivation of a mass shooter's intent to do harm can help practitioners and policy makers develop more effective intervention strategies., Objective: To examine the prevalence of communication of intent to do harm, known as leakage, in a sample of 170 mass public shooters from 1966 to 2019; the characteristics of perpetrators who do and do not leak their plans; and whether leakage is a form of fame-seeking behavior or a cry for help among individuals who are in crisis or suicidal., Design, Setting, and Participants: This cross-sectional study included perpetrators who killed 4 or more people in a public space from 1996 to 2019 and were included in a comprehensive database of US mass shootings. That database was built from August 2017 to December 2019, and analysis took place from January to May 2021., Main Outcomes and Measures: Leakage was identified and coded using publicly available records. Any communication to a third party of an intent to do harm prior to the shooting was coded as leakage. Logistic regression models were used to examine the risk factors associated with leakage. Models estimating leakage were examined to assess the 2 hypothesized pathways to leakage (the cry-for-help model and the fame-seeking model)., Results: The 170 participants in this sample included 166 (97.7%) male perpetrators and 3 (2.3%) female perpetrators, with a mean (SD) age of 34 (12) years. Overall, 161 participants had known race and ethnicity: 11 (6.8%) Asian individuals, 35 (21.7%) Black individuals, 14 (8.7%) Latinx individuals, 7 (4.4%) Middle Eastern individuals, 3 (1.9%) Native American individuals, 89 (55.3%) White individuals, and 2 (1.2%) individuals with other race and ethnicity. Overall, 79 mass shooters (46.5%) leaked their plans. Of perpetrators who leaked their plans, 35 (44.3%) leaked specific plans about a mass shooting, and 44 (55.1%) leaked nonspecific plans about generalized violence. The study findings indicate that leakage was associated with receiving counseling (odds ratio, 7.0; 95% CI, 2.0-24.8) and suicidality (odds ratio, 3.7; 95% CI, 1.0-13.6), suggesting that leakage may best be characterized as a cry for help from perpetrators prior to their act., Conclusions and Relevance: In this study, nearly half of the perpetrators of mass shootings leaked their plans. Leakage was associated with receiving counseling and suicidality. Leakage is a critical moment for mental health intervention to prevent gun violence. Opportunities to report threats of violence need to be increased. Traditional threat assessment models focused on specific threats of violence may miss critical opportunities for intervention.

A melt-salt-assisted method is utilized to construct an onion-like hybrid with Co/CoO nanoparticles embedded in graphitic Fe-N-doped carbon shells (Co/CoO@FeNC) as a bifunctional electrocatalyst. The iron-polypyrrole (Fe-PPy) is firstly prepared with a reverse emulsion. Direct pyrolysis of Fe-PPy yields turbostratic Fe-N-doped carbon (FeNC) with excellent oxygen reduction reaction (ORR) electrocatalysis, while the melt salt (CoCl 2 ) mediated pyrolysis of Fe-PPy obtains onion-like Co/CoO@FeNC with a reversible overvoltage value of 0.695 V, largely superior to Pt/C and IrO 2 (0.771 V) and other Co-based catalysts reported so far. The ORR activity is mainly due to the graphitic FeNC and further enhanced by CoN x bonds, whereas the oxygen evolution reaction (OER) activity is principally due to the Co/CoO composite. Concurrently, Co/CoO@FeNC as cathode catalyst enables Zn-air battery with a high open circuit voltage of 1.42 V, a peak power density of 132.8 mW cm -2 , a specific capacity of 813 mAh g Zn -1 , and long-term stability., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2021 Elsevier Inc. All rights reserved.)

Maize is a cold sensitive crop that exhibits severe retardation of growth and development when exposed to cold spells during and right after germination, including the slowdown in development of new leaves and in formation of the photosynthetic apparatus. Improving cold tolerance in maize would allow early sowing to improve crop yield by prolonging a growing season and by decreasing the negative effects of summer drought, diseases, and pests. Two maize inbreds widely incorporated into American maize germplasm, B73 and Mo17, exhibit different levels of tolerance to low temperature exposure at seedling stage. In addition, thirty seven diverse inbred maize lines showed large variation for seedling response to low temperature exposure with lines with extremely low tolerance to seedling exposure to low temperatures falling into stiff stalk, non-stiff stalk, and tropical clades. We employed the maize intermated B73×Mo17 (IBM) recombinant inbred line population (IBM Syn4 RIL) to investigate the genetic architecture of cold stress tolerance at a young seedling stage and to identify quantitative trait loci (QTLs) controlling this variation. A panel of 97 recombinant inbred lines of IBM Syn4 were used to measure, and score based on several traits related to chlorophyll concentration, leaf color, and tissue damage. Our analysis resulted in detection of two QTLs with high additive impact, one on chromosome 1 (bin 1.02) and second on chromosome 5 (bin 5.05). Further investigation of the QTL regions using gene expression data provided a list of the candidate genes likely contributing to the variation in cold stress response. Among the genes located within QTL regions identified in this study and differentially expressed in response to low temperature exposure are the genes with putative functions related to auxin and gibberellin response, as well as general abiotic stress response, and genes coding for proteins with broad regulatory functions., Competing Interests: The authors have declared that no competing interests exist.