The current article aimed to examine the effect of organic and biofertilizers on the growth and yield of lavender essential oil (Lavandula Officinalis L.). This experiment was conducted over two consecutive seasons in 2018/2019 and 2019/2020 at the net house of the Central Laboratory of the Agricultural Climate Research Center in Dokki, Giza Governorate, Egypt. To assess the effects of biofertilizers (Azospirillum spp., Azotobacter spp.) and active dry yeast on the vegetative development, chemical composition, yield, and essential oil concentration of lavender. The obtained results showed that, treated plants with compost + Azospirillum spp. + Azotobacter spp. was the superior treatment, which improved plant growth characters i.e., plant height, number of branches, fresh and dry weights/herb. Chlorophyll a, b and carotenoids concentrations, the phenolic compounds, flavonoid content, total carbohydrates content in dry matter of herb, Nitrogen, Phosphorus and Potassium, total free amino acids, the total antioxidants and the endogenous hormones in all cuts. Essential oil content carried out to evaluate the quality of oil. Also, significant increase was showed with active dry yeast. [ABSTRACT FROM AUTHOR]