Search

Your search keyword '"Hamanaka, Sanae"' showing total 27 results
Start Over Author "Hamanaka, Sanae"
27 results on '"Hamanaka, Sanae"'

2. In vitro and in vivo functions of T cells produced in complemented thymi of chimeric mice generated by blastocyst complementation

Author

Yamazaki, Kazuto, Kubara, Kenji, Ishii, Satoko, Li, Peng, Dairiki, Ryo, Hihara, Taro, Ishizuka, Yuta, Izumi, Yukina, Kumai, Minoru, Kamisako, Tsutomu, Ishizaki, Hiroyoshi, Sato, Hideyuki, Masaki, Hideki, Mizuno, Naoaki, Mitsuhashi, Kaoru, Ito, Masashi, Hamanaka, Sanae, Yamaguchi, Tomoyuki, Watanabe, Motoo, Sugiyama, Fumihiro, and Nakauchi, Hiromitsu

Published
2022
Full Text
View/download PDF

9. Compensation of Disabled Organogeneses in Genetically Modified Pig Fetuses by Blastocyst Complementation

Author

Matsunari, Hitomi, primary, Watanabe, Masahito, additional, Hasegawa, Koki, additional, Uchikura, Ayuko, additional, Nakano, Kazuaki, additional, Umeyama, Kazuhiro, additional, Masaki, Hideki, additional, Hamanaka, Sanae, additional, Yamaguchi, Tomoyuki, additional, Nagaya, Masaki, additional, Nishinakamura, Ryuichi, additional, Nakauchi, Hiromitsu, additional, and Nagashima, Hiroshi, additional

Published
2020
Full Text
View/download PDF

11. Generation of Vascular Endothelial Cells and Hematopoietic Cells by Blastocyst Complementation

Author

Hamanaka, Sanae, primary, Umino, Ayumi, additional, Sato, Hideyuki, additional, Hayama, Tomonari, additional, Yanagida, Ayaka, additional, Mizuno, Naoaki, additional, Kobayashi, Toshihiro, additional, Kasai, Mariko, additional, Suchy, Fabian Patrik, additional, Yamazaki, Satoshi, additional, Masaki, Hideki, additional, Yamaguchi, Tomoyuki, additional, and Nakauchi, Hiromitsu, additional

Published
2018
Full Text
View/download PDF

12. In vitro platform of allogeneic stem cell-derived cardiomyocyte transplantation for cardiac conduction defects

Author

Yoshida, Akira, primary, Lee, Jong-Kook, additional, Tomoyama, Satoki, additional, Miwa, Keiko, additional, Shirakawa, Keiichi, additional, Hamanaka, Sanae, additional, Yamaguchi, Tomoyuki, additional, Nakauchi, Hiromitsu, additional, Miyagawa, Shigeru, additional, Sawa, Yoshiki, additional, Komuro, Issei, additional, and Sakata, Yasushi, additional

Published
2018
Full Text
View/download PDF

13. Practical selection methods for rat and mouse round spermatids without DNA staining by flow cytometric cell sorting

Author

Hayama, Tomonari, primary, Yamaguchi, Tomoyuki, additional, Kato‐Itoh, Megumi, additional, Ishii, Yumiko, additional, Mizuno, Naoaki, additional, Umino, Ayumi, additional, Sato, Hideyuki, additional, Sanbo, Makoto, additional, Hamanaka, Sanae, additional, Masaki, Hideki, additional, Hirabayashi, Masumi, additional, and Nakauchi, Hiromitsu, additional

Published
2016
Full Text
View/download PDF

15. Generation of Mouse Functional Oocytes in Rat by Xeno-Ectopic Transplantation of Primordial Germ Cells1

Author

Hayama, Tomonari, primary, Yamaguchi, Tomoyuki, additional, Kato-Itoh, Megumi, additional, Hamanaka, Sanae, additional, Kawarai, Mami, additional, Sanbo, Makoto, additional, Tamura, Chihiro, additional, Lee, Youn-Su, additional, Yanagida, Ayaka, additional, Murayama, Hideyuki, additional, Mizuno, Naoaki, additional, Umino, Ayumi, additional, Sato, Hideyuki, additional, Yamazaki, Satoshi, additional, Masaki, Hideki, additional, Kobayashi, Toshihiro, additional, Hirabayashi, Masumi, additional, and Nakauchi, Hiromitsu, additional

Published
2014
Full Text
View/download PDF

19. Development of an All-in-One Inducible Lentiviral Vector for Gene Specific Analysis of Reprogramming

Author

Yamaguchi, Tomoyuki, primary, Hamanaka, Sanae, additional, Kamiya, Akihide, additional, Okabe, Motohito, additional, Kawarai, Mami, additional, Wakiyama, Yukiko, additional, Umino, Ayumi, additional, Hayama, Tomonari, additional, Sato, Hideyuki, additional, Lee, Youn-Su, additional, Kato-Itoh, Megumi, additional, Masaki, Hideki, additional, Kobayashi, Toshihiro, additional, Yamazaki, Satoshi, additional, and Nakauchi, Hiromitsu, additional

Published
2012
Full Text
View/download PDF

21. Generation of Germline-Competent Rat Induced Pluripotent Stem Cells

Author

Hamanaka, Sanae, primary, Yamaguchi, Tomoyuki, additional, Kobayashi, Toshihiro, additional, Kato-Itoh, Megumi, additional, Yamazaki, Satoshi, additional, Sato, Hideyuki, additional, Umino, Ayumi, additional, Wakiyama, Yukiko, additional, Arai, Mami, additional, Sanbo, Makoto, additional, Hirabayashi, Masumi, additional, and Nakauchi, Hiromitsu, additional

Published
2011
Full Text
View/download PDF

22. Generation of Rat Pancreas in Mouse by Interspecific Blastocyst Injection of Pluripotent Stem Cells

Author

Kobayashi, Toshihiro, primary, Yamaguchi, Tomoyuki, additional, Hamanaka, Sanae, additional, Kato-Itoh, Megumi, additional, Yamazaki, Yuji, additional, Ibata, Makoto, additional, Sato, Hideyuki, additional, Lee, Youn-Su, additional, Usui, Jo-ichi, additional, Knisely, A.S., additional, Hirabayashi, Masumi, additional, and Nakauchi, Hiromitsu, additional

Published
2010
Full Text
View/download PDF

27. In vitro platform of allogeneic stem cell-derived cardiomyocyte transplantation for cardiac conduction defects.

Author

Yoshida A, Lee JK, Tomoyama S, Miwa K, Shirakawa K, Hamanaka S, Yamaguchi T, Nakauchi H, Miyagawa S, Sawa Y, Komuro I, and Sakata Y

Subjects
Activins pharmacology, Allogeneic Cells, Animals, Animals, Newborn, Benzothiazoles pharmacology, Bone Morphogenetic Protein 4 pharmacology, Calmodulin-Binding Proteins metabolism, Cell Differentiation, Connexin 43 metabolism, Electrophysiological Phenomena, Flow Cytometry, Heart Ventricles cytology, In Vitro Techniques, Induced Pluripotent Stem Cells drug effects, Myocytes, Cardiac cytology, Myocytes, Cardiac transplantation, Rats, Sarcomeres, Transplantation, Homologous, Action Potentials physiology, Cardiac Conduction System Disease therapy, Induced Pluripotent Stem Cells cytology, Myocytes, Cardiac physiology
Abstract

Aims: The aim of the present study is to develop in vitro experimental analytical method for the electrophysiological properties of allogeneic induced pluripotent stem cell-derived cardiomyocytes (CMs) in cardiac conduction defect model., Methods and Results: Cardiomyocytes were derived from rat induced pluripotent stem cells CMs (riPSC-CMs) using an embryoid body-based differentiation method with the serial application of growth factors including activin-A, bone morphogenetic protein 4 (BMP-4), and inhibitor of wnt production 2 (IWP-2). Flow cytometry analysis showed that 74.0 ± 2.7% of riPSC-CMs expressed cardiac troponin-T (n = 3). Immunostaining analysis revealed organized sarcomeric structure in riPSC-CMs and the expression of connexin 43 between riPSC-CMs and neonatal rat ventricular CMs (NRVMs). Ca2+ transient recordings revealed the simultaneous excitement of riPSC-CMs and NRVMs, and prolonged Ca2+ transient duration of riPSC-CMs as compared with NRVMs (731 ± 15.9 vs. 610 ± 7.72 ms, P < 0.01, n = 3). Isolated NRVMs were cultured in two discrete regions to mimic cardiac conduction defects on multi-electrode array dish, and riPSC-CMs were seeded in the channel between the two discrete regions. Membrane potential imaging with di-8-ANEPPS discerned the propagation of the electrical impulse from one NRVM region to the other through a riPSC-CM pathway. This pathway had significantly longer action potential duration as compared with NRVMs. Electrophysiological studies using a multi-electrode array platform demonstrated the longer conduction time and functional refractory period of the riPSC-CM pathway compared with the NRVM pathway., Conclusion: Using an in vitro experimental system to mimic cardiac conduction defect, transplanted allogeneic riPSC-CMs showed electrical coupling between two discrete regions of NRVMs. Electrophysiological testing using our platform will enable electrophysiological screening prior to transplantation of stem cell-derived CMs.

Published
2018
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results