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Q345B/2205 dissimilar steel multi-layer multi-pass welded joints were prepared using gas metal arc welding (GMAW). The effects of heat inputs on the microstructures and properties of welded joints were studied. The morphology and composition distribution of Q345B base metal near the fusion line were investigated. The results showed that the welded joints under different heat inputs were all present “carbon depleted zone” and “carburized zones” on both sides of the Q345B base metal and transition layer interface. The microstructure of the “carburized zones” was composed of martensite phase and granular bainite phase. It was found that only the welded joints with heat inputs of 1.765 kJ/mm and 2.860 kJ/mm met the usage requirements. The corrosion behavior and characteristics of the welded joint with a heat input of 1.765 kJ/mm in a 3.5 wt % NaCl solution were investigated intensively. During the soaked process of 14 days, the total impedance value of the welded joint showed first increasing and then decreasing. The maximum value of 8.7 kΩ cm2 was obtained on the 5th day. As the soaked time increased, the corrosion products of the welded joint underwent the formation, thickening, cracking and detachment, which was the main reason of the change in the corrosion resistance. The research results of this article have significant implications for the application of low-alloy steel and stainless steel welded joints in engineering.

Abstract This study aimed to elucidate the molecular mechanisms by which PLD2 controls apoptosis and edema in pancreatic cells via the Nrf2/NF-κB pathway. AR42J rat pancreatic cells were treated with 10 nM mitomycin to create an in vitro pancreatitis model (model group), with a control group receiving phosphate-buffered saline. Cells were transfected with a PLD2 overexpression plasmid using Lipofectamine 3000, forming the PLD2 overexpression group. PLD2 protein expression was assessed by Western blotting, and TNF-α, IL-6, and IL-10 levels were measured by RT-qPCR. Nrf2/NF-κB protein expressions were also analyzed. Apoptosis and necrosis were evaluated using Annexin V-FITC/PI staining and the LDH release test. Cell edema was assessed by cell volume, ion content, and membrane damage. Western blotting was used to analyze pan-apoptosis-related proteins. PLD2 expression was lower in the model group compared to controls (P

Recent years have seen the Ulva green tide and Sargassum golden tide become commonplace in the coastal waters of China. However, little is known on how the combination of ocean warming and eutrophication would affect the interaction of green and golden tides. In this study, we cultured the green tide alga Ulva prolifera and the golden tide alga Sargassum horneri under different temperatures (5, 10, 15, 20, 25, and 30°C) and two nutrient concentrations (Low nutrient, LN: 5 μM-nitrate and 0.5 μM-phosphate; High nutrient, HN: 500 μM-N and 50 μM-P) in both monoculture and coculture systems to investigate the physiological responses and their competitive relationships. In monocultures, the growth of U. prolifera and S. horneri, along with pigment concentrations and photosynthesis, increased with rising temperature, reaching a plateau at 15 - 25°C. However, when the temperature increased to 30°C, the growth of U. prolifera and S. horneri decreased abruptly, with S. horneri even suffering death. In coculture, the growth of both U. prolifera and S. horneri was inhibited compared to the monoculture, with the greatest decline observed in S. horneri at 25°C under two nutrient conditions. Our results show that U. prolifera would outcompete S. horneri under high temperature in coculture, suggesting that ocean warming would enhance the competitive advantage of green tide over golden tide under eutrophication in the future.

Benzophenone-4 (BP-4), a widely utilized organic ultraviolet (UV) filter, is recognized as a pseudo-persistent contaminant in aquatic environments. To elucidate the effects and mechanisms of BP-4 on marine diatoms, an investigation was conducted on the growth rate, photosynthetic pigment content, photosynthetic parameters, antioxidant enzyme activity, malondialdehyde (MDA) levels, cellular structure, and transcriptome profile of the model species, Phaeodactylum tricornutum. The results showed a pronounced inhibition of algal growth upon exposure to BP-4, with a 144 h-EC50 value of 201 mg·L−1. In addition, BP-4 exposure resulted in a significant reduction in biomass, disruption of cell membrane integrity, and increased MDA accumulation, with levels escalating 3.57-fold at 125 mg·L−1 of BP-4. In the BP-4-treated samples, 1556 differentially expressed genes (DEGs) were identified, of which 985 were upregulated and 571 were downregulated. Gene ontology and KEGG pathway enrichment analysis revealed that the carbon fixation and carbon metabolism processes in P. tricornatum were disrupted in response to BP-4 exposure, along with excessive reactive oxygen species (ROS) production. The upregulation of genes associated with photosynthetic pigment (chlorophyll and carotenoids) synthesis, phospholipid synthesis, ribosome biogenesis, and translation-related pathways may be regarded as a component of P. tricornatum's tolerance mechanism towards BP-4. These results provide preliminary insights into the toxicity and tolerance mechanisms of BP-4 on P. tricornatum. They will contribute to a better understanding of the ecotoxicological impacts of BP-4 on the marine ecosystem and provide valuable information for elimination of BP-4 in aquatic environment by bioremediation.

Abstract Lack of liver regenerative capacity is the primary cause of hepatic failure and even mortality in patients undergoing hepatectomy, with no effective intervention strategies currently available. Therefore, identifying efficacious interventions to enhance liver regeneration is pivotal for optimizing clinical outcomes. Recent studies have demonstrated that vagotomy exerts an inhibitory effect on liver regeneration following partial hepatectomy, thereby substantiating the pivotal role played by the vagus nerve in the process of liver regeneration. In recent years, electroacupuncture (EA) has emerged as a non‐invasive technique for stimulating the vagus nerve. However, EA on hepatic regeneration remains uncertain. In this study, a 70% partial hepatectomy (PH) mouse model is utilized to investigate the effects of EA on acute liver regeneration and elucidate its underlying molecular mechanisms. It is observed that EA at ST36 acutely activated cholinergic neurons in the dorsal motor nucleus of the vagus nerve (DMV), resulting in increased release of acetylcholine from hepatic vagal nerve endings and subsequent activation of IL‐6 signaling in liver macrophages. Ultimately, these events promoted hepatocyte proliferation and facilitated liver regeneration. These findings provide insights into the fundamental brain‐liver axis mechanism through which EA promotes liver regeneration, offering a novel therapeutic approach for post‐hepatectomy liver regeneration disorders.

The Electric Fish Optimization (EFO) algorithm is inspired by the predation behavior and communication of weak electric fish. It is a novel meta-heuristic algorithm that attracts researchers because it has few tunable parameters, high robustness, and strong global search capabilities. Nevertheless, when operating in complex environments, the EFO algorithm encounters several challenges including premature convergence, susceptibility to local optima, and issues related to passive electric field localization stagnation. To address these challenges, this study introduces Adaptive Electric Fish Optimization Algorithm Based on Standstill Label and Level Flight (SLLF-EFO). This hybrid approach incorporates the Golden Sine Algorithm and good point set theory to augment the EFO algorithm’s capabilities, employs a variable-step-size Levy flight strategy to efficiently address passive electric field localization stagnation problems, and utilizes a standstill label strategy to mitigate the algorithm’s tendency to fall into local optima during the iterative process. By leveraging multiple solutions to optimize the EFO algorithm, this framework enhances its adaptability in complex environments. Experimental results from benchmark functions reveal that the proposed SLLF-EFO algorithm exhibits improved performance in complex settings, demonstrating enhanced search speed and optimization accuracy. This comprehensive optimization not only enhances the robustness and reliability of the EFO algorithm but also provides valuable insights for its future applications.

Abstract Active electrolocation organ of weakly electric fish act as a proximity detection system with high accuracy in recognizing object parameters such as size and shape. In contrast, some fish with passive electrolocation organ are able to detect objects at a greater range. This paper proposes a joint active–passive electrolocation algorithm for long-range and high-precision underwater localization, inspired by the active and passive electroreceptive organs of fish. The study begins by designing a large experimental platform for the underwater localization system to investigate the response of underwater objects to active and passive electric fields. Based on the response, the paper proposes separate underwater active and passive electrolocation algorithms, which are then combined to form a joint algorithm. Experimental results demonstrate that the proposed algorithm achieves high localization accuracy and long detection distance. The joint active–passive electrolocation algorithm has potential applications in submarine resource exploration, underwater robotics, and maritime military projects, while also providing new ideas for future research on long-range underwater object detection and identification based on electrolocation.

Macroalgal biomass blooms, including those causing the green and golden tides, have been rising along Chinese coasts, resulting in considerable social impacts and economic losses. To understand the links between the ongoing climate changes (ocean warming and acidification) and algal tide formation, the effects of temperature (20 and 24 °C), pCO2 concentration (Partial Pressure of Carbon Dioxide, 410 ppm and 1000 ppm) and their interaction on the growth of Ulva prolifera and Ulva lactuca (green tide forming species), as well as Sargassum horneri (golden tide forming species) were investigated. The results indicate that the concurrent rises in temperature and pCO2 level significantly boosted the growth and nutrient uptake rates of U. lactuca. For U. prolifera, the heightened growth and photosynthetic efficiency under higher CO2 conditions are likely due to the increased availability of inorganic carbon. In contrast, S. horneri exhibited negligible responsiveness to the individual and combined effects of the increased temperature and CO2 concentration. These outcomes indicate that the progressive climate changes, characterized by ocean warming and acidification, are likely to escalate the incidence of green tides caused by Ulva species, whereas they are not anticipated to precipitate golden tides.

In order to test the influence of the structural design of a no-tillage corn precision planter on vibration stability performance, a vibration model was constructed with the help of MATLAB/Simulink, and it was concluded that the vibration response curve of the no-tillage corn precision planter was relatively smooth. Based on the theory of the discrete element method (DEM), taking the planting apparatus of the no-tillage corn precision planter as the research object, firstly, a DEM single-factor test was carried out to investigate the effects of the slot inclination angle, number of slots, and rotational speed of the planter plate on the disturbance performance. Then, a three-factor, three-level orthogonal test was conducted with the maximum amount of seed discharging, the minimum average speed, and the minimum average kinetic energy as the final optimization objectives, and the qualified rate of seed discharging and the leakage rate as the evaluation indexes. The results show that the larger the inclination angle, the higher the number of slots, and the faster the rotational speed, the more violent the particle disturbance. At the same time, when the slot inclination angle of the planter plate is 60°, the number of slots is 20, and the rotational speed is 55 rpm, the seed discharge efficiency is the highest, at this time, the seed discharge qualification rate of maize particles is 95%, and the leakage rate is 3%; the results of this test can provide technical support for the research of the same kind of precision sowing equipment in the future.

Abstract Background Recently, periumbilical fat (PF) mass, an autologous material with a high survival rate, has been transplanted to treat sunken or dissatisfactory double eyelids. However, the intricate complications of PF grafts and associated reconstructive strategies are infrequently discussed. Methods During 3 years, 20 patients (33 eyes) with eyelid malformations caused by PF grafts into the orbital septum or on the surface of the levator aponeurosis underwent corrective blepharoplasty. We recorded patients’ subjective feelings and identified deformities from crease abnormalities, bloated appearance, and problems with the eyelid’s height. Then, we categorize them into three types based on their complexity: type I, swollen appearance; type II, obvious adhesion; type III, severe comprehensive damage. The relevant management included removing fat implants, releasing the adhesion, and rebuilding the physical structure according to the anatomic damage mechanism. The improvement effect was assessed with a satisfaction survey from patients and doctors at 6 months of follow-up. Results The swollen appearance was observed in 26 eyes (78.8%), an unsmooth double-eyelid line was in 23 eyes (69.7%), and the incidence of adhesion was in 22 eyes (66.7%). Following a comprehensive evaluation, 15 eyes (45.5%) and 13 (39.4%) were classified as type I and type II respectively. After the 6-month follow-up, 22 eyes (66.7%) showed exceptional aesthetic results, whereas only 2 eyes as type III had a poor outcome. Conclusions The deformities emerging from periumbilical fat into the upper eyelid are associated with the shape of the fat and the adhesion in tissues. Graft removal, adhesion release, and restoration of the natural anatomic structure can have positive outcomes.

Forest soils play a vital role in purifying exogenous nitrogen ions and mitigating eutrophication in downstream water bodies. However, the heterogeneity of nitrogen ion removal efficiency in different forest soils and the underlying driving mechanisms remain not fully known. In our research, we selected soils from four typical forest stands, namely, Pinus massoniana-Quercus variabilis mixed forest (MF), Quercus variabilis forest (QF), Pinus massoniana forest (PF), and Citrus reticulata forest (CF), surrounding China’s second largest reservoir in the Yangtze River Basin. Using laboratory simulation experiments, we observed variations in concentration of ammonium nitrogen (NH4+-N) and nitrate nitrogen (NO3−-N) in soil leachate after addition of exogenous NH4+-N and NO3−-N solutions, along with the corresponding physical, chemical, and microbial factors. The results showed that on Day 21, all soils exhibited comparable removal rates of approximately 93% for NH4+-N and 70% for NO3−-N. On Day 4, 6–15, PF and QF demonstrated significantly higher removal efficiencies for NH4+-N and NO3−-N, respectively. Combining direct with indirect effects from Partial Least Squares Path Modeling (PLS-PM), NH4+-N removal efficiency was promoted by total organic carbon (TOC), soil NO3−-N and NO2−-N, while it was inhibited by soil water content (SWC), urease activity, soil NH4+-N, and ammonia-oxidizing archaea (AOA). NO3−-N removal efficiency was facilitated by SWC, TOC, and urease, while it was restrained by soil NO3−-N and ammonia-oxidizing bacteria (AOB). Notably, TOC was the only factor that had a comprehensive promoting effect on both NH4+-N and NO3−-N removal efficiency. Our study indicates that soil in coniferous forest excels in NH4+-N removal while broadleaf forest in NO3−-N, and the spatial layout of water source forests should consider water replenishment time, the form of nitrogen ion pollutants, and the nitrogen purification features of different forest types when the prime objective is to purity the water. Additionally, enhancing soil organic carbon could be an effective forest management approach to improve soil nitrogen ion purification and mitigate agricultural and nitrogen deposition pollution.

Abstract Background Near 70% of hepatocellular carcinoma (HCC) recurrence is early recurrence within 2-year post surgery. Long non-coding RNAs (lncRNAs) are intensively involved in HCC progression and serve as biomarkers for HCC prognosis. The aim of this study is to construct a lncRNA-based signature for predicting HCC early recurrence. Methods Data of RNA expression and associated clinical information were accessed from The Cancer Genome Atlas Liver Hepatocellular Carcinoma (TCGA-LIHC) database. Recurrence associated differentially expressed lncRNAs (DELncs) were determined by three DEG methods and two survival analyses methods. DELncs involved in the signature were selected by three machine learning methods and multivariate Cox analysis. Additionally, the signature was validated in a cohort of HCC patients from an external source. In order to gain insight into the biological functions of this signature, gene sets enrichment analyses, immune infiltration analyses, as well as immune and drug therapy prediction analyses were conducted. Results A 4-lncRNA signature consisting of AC108463.1, AF131217.1, CMB9-22P13.1, TMCC1-AS1 was constructed. Patients in the high-risk group showed significantly higher early recurrence rate compared to those in the low-risk group. Combination of the signature, AFP and TNM further improved the early HCC recurrence predictive performance. Several molecular pathways and gene sets associated with HCC pathogenesis are enriched in the high-risk group. Antitumor immune cells, such as activated B cell, type 1 T helper cell, natural killer cell and effective memory CD8 T cell are enriched in patients with low-risk HCCs. HCC patients in the low- and high-risk group had differential sensitivities to various antitumor drugs. Finally, predictive performance of this signature was validated in an external cohort of patients with HCC. Conclusion Combined with TNM and AFP, the 4-lncRNA signature presents excellent predictability of HCC early recurrence.

Abstract Background Deer antler is a traditional Chinese medicine with the function of tonifying kidney and strengthening bone, which is often used to treat orthopedic diseases. Methods Eight-week-old C57BL/6 mice were used as the fixation model of open tibial fracture with intramedullary nail. The mice were treated with deer antler extract (DAE) or PBS by oral gavage once daily. The tibial fracture samples were collected and performed to the tissue analysis, including X-ray, micro-CT, histology, qRT-PCR, immunohistochemistry. MC3T3-E1 cells were used to detect the effect of deer antler extract on ability of cell proliferation and migration by CCK-8 assay and cell scratch test. Results Imaging and micro-CT showed that DAE could promote the healing of tibial fracture in mice, and histological analysis showed that DAE could promote the transformation of cartilage callus to bone callus in fracture area. The results of qRT-PCR and immunohistochemistry showed that DAE could promote intrachondral ossification in fracture zone and the mechanism of promoting fracture healing may be related to the activation of BMP-2/SMAD4 signaling pathway. In the cytological experiment of DAE, it can be found that DAE promoted the proliferation of MC3T3-E1 cells and the migration of MC3T3-E1 cells at a certain concentration, which is also related to the promotion of fracture healing by DAE. Conclusion DAE can promote fracture healing by activating BMP-2/SMAD4 signaling pathway. DAE has the potential to be used in clinic as an important means of promoting fracture healing.

Based on the technique of the discrete one-turn transfer maps, the problem of linear coupling between horizontal and vertical betatron oscillations in an accelerator has been treated exactly and entirely in explicit form. The stability region in the fractional part of the horizontal and the vertical betatron tune space as a function of the linear coupling strength has been obtained, and the increment/decrement of the horizontal and the vertical betatron oscillations in the case of the linear sum resonance has been shown to be approximately equal to the half of the coupling strength. The normal form parameterization of the one-turn linear map with horizontal-to-vertical coupling has been developed in detail in the spirit of Edwards and Teng formalism. The motion in the normal mode in the new normal form coordinates is decoupled by implying that two independent Courant-Snyder invariants exist, which have been found explicitly.

Abstract The gut microbiota of amphibians plays a crucial role in maintaining health and adapting to various developmental stages. The composition of gut microbial community is influenced by the phylogeny, habitat, diet, and developmental stage of the host. The present study analyzed the microbiota in the intestine of O. tormota at 11 developmental stages (from the tadpole at Gosner stage 24 to the 3‐year‐old adult) using high‐throughput 16S rRNA sequencing. Alpha diversity index analysis of the microbiota revealed that the index decreased from tadpole at Gosner stage 24 to adult frog stage, remained stable during the adult frog stages, but increased significantly at the early metamorphosis and hibernation preparation stages. The gut microbiota structure is similar in adult frogs but differs significantly in other developmental stages. Furthermore, the dominant phyla of gut microbiota in tadpoles were Proteobacteria, Firmicutes, Actinobacteria, and Bacteroidetes, whereas those in adult frogs were Proteobacteria, Firmicutes, Bacteroidetes, and Verrucomicrobia. Host and environmental factors jointly affected the gut microbial diversity and community composition of O. tormota, but developmental stage, feeding habit, and habitat type had a more significant influence. The microbial community in the gut varies with the developmental stage of the host and constantly adapts to the survival requirements of the host. These findings advance our understanding of the evolutionary mechanism of amphibian gut microbiota in maintaining health homeostasis and adaptation.

Abstract Background Acute pancreatitis (AP) is an inflammatory process unexpectedly occurring in the pancreas, imposing a substantial burden on healthcare systems. Herein, we aimed to clarify the mechanism of action of phospholipase D2 (PLD2) in cerulein‐treated AR42J cells, affording valuable insights into the treatment of AP. Methods The levels of PLD2, miR‐5132‐5p, inflammatory factors (interleukin [IL]−10, IL‐6, and tumor necrosis factor‐α), caspase‐3 activity, and apoptosis‐related proteins (Bax and Bcl‐2) in cerulein‐treated AR42J cells were detected using reverse transcription‐quantitative polymerase chain, caspase‐3 activity, and Western blot analysis. Protein levels of nuclear Factor erythroid 2‐Related Factor 2 (Nrf2) and nuclear factor‐k‐gene binding (NF‐κB) were detected by Western blot analysis. TargetScan predicted upstream microRNAs (miRNAs) of PLD2, and the interaction between miR‐5132‐5p and PLD2 was verified using a luciferase assay. Results In cerulein‐treated AR42J cells, PLD2 levels were downregulated, while miR‐5132‐5p expression was upregulated. Overexpression of PLD2 attenuated the cerulein‐mediated facilitatory effect on inflammation and apoptosis in AR42J cells by regulating the Nrf2/NFκB pathway. Luciferase reporter analysis revealed that miR‐5132‐5p targeted PLD2, and miR‐5132‐5p negatively regulated PLD2. Upregulation of miR‐5132‐5p expression exacerbated inflammation and apoptosis and reversed the protective effect of PLD2 overexpression on AP. Conclusion PLD2 targeted by miR‐5132‐5p can attenuate cerulein‐induced AP in AR42J cells via the Nrf2/NFκB pathway, providing therapeutic targets for patients with AP.

Macroalgae, playing a crucial role in coastal marine ecosystems, are subject to multiple environmental challenges due to tidal and seasonal alterations. In this work, we investigated the physiological responses of Pyropia yezoensis to ocean acidification (ambient CO2 (AC: 400 μatm) and elevated CO2 (HC: 1000 μatm)) under changing salinity (20, 30 psu) and light intensities (50, 100 μmol photons m−2 s−1) by measuring the growth, pigment content, chlorophyll fluorescence, and soluble sugar content. The key results are the following: (1) P. yezoensis exhibited better growth under normal salinity (30 psu) compared to hyposaline conditions (20 psu). (2) Intermediate light intensity increased phycoerythrin content, ultimately enhancing thalli growth without significant changes to the contents of chlorophyll a and carotenoids. (3) Ocean acidification alleviated hyposaline stress by enhancing pigment production in P. yezoensis only at a salinity of 20 psu, highlighting the complex interplay of these environmental factors. These findings indicate that higher light intensities and elevated pCO2 levels could mitigate the stress caused by low salinity.

Background: Immune evaluation of biomaterials for tissue regeneration is a critical preclinical evaluation. The current evaluation criterion (ISO 10993-1 or GB/T 16886) uses rodents to perform the immune evaluation. However, the immune system of rodents is different from humans, the obtained results may not be reliable, which could lead directly to the failure of clinical trials. Granulocyte-macrophage colony-stimulating factor (GM-CSF) shows a great potential application in tissue regeneration by regulating local immune responses. The presented work combines the advantages of GM-CSF (immunoregulation) and hierarchically 3D nanofiber scaffolds (tissue regeneration).Methods: Firstly, we fabricated GM-CSF loaded 3D radially aligned nanofiber scaffolds, and then subcutaneous implantation was performed in humanized mice. The whole scaffold and surrounding tissue were harvested at each indicated time point. Finally, the cell infiltration and local immune responses were detected by histological observations, including H&E and Masson staining and immunochemistry.Results: We found significant cell migration and extracellular matrix deposition within the 3D radially aligned nanofiber scaffold after subcutaneous implantation. The locally released GM-CSF could accelerate the expression of human dendritic cells (CD11c) only 3 days after subcutaneous implantation. Moreover, higher expression of human cytotoxic T cells (CD3+/CD8+), M1 macrophages (CD68/CCR7) was detected within GM-CSF loaded radially aligned nanofiber scaffolds and their surrounding tissues.Conclusions: The 3D radially aligned scaffold can accelerate cell migration from surrounding tissues to regenerate the wound area. And the locally released GM-CSF enhances dendritic cell recruitment and activation of cytotoxic T cells and M1 macrophages. Taken together, the GM-CSF loaded 3D radially aligned nanofiber scaffolds have a promising potential for achieving tissue regeneration.

Abstract The competitive relationship and coexistence pattern among close related species have long been one of the hot issues in ecological research. Interspecies interactions can exert important influences on the local distribution of rare species. Black muntjac Muntiacus crinifrons is an endemic species to eastern China, currently restricted to limited regions. In contrast, Chinese muntjac Muntiacus reevesi is the most common and widespread deer in southern China. Both species co‐occur in southern Anhui and western Zhejiang Province. Little is known about the interaction of these two sympatric‐related species. In this study, to investigate the site use determinants and co‐occurrence pattern of the two sympatric muntjac species, we conducted a camera trap survey across about 250 km2 in mountainous area of southern Anhui Province, China. We adopted a multistep approach to incorporate habitat preferences while modeling occupancy and detection. We found that the two species did not separate along elevation gradient (range from 400 m to 1,400 m) as described in previous studies. Results of single‐species occupancy models indicated that elevation had positive effects on the site use of both species, while slope had an opposite influence on their site use. Positive effects of elevation on the site use implied that both species try to avoid human interference at low elevations. Significant negative effect of slope on the site use of black muntjac suggested that the species prefer habitat with gentle slope and avoided steep. Co‐occurrence models and species interaction factors provided evidence that the two muntjac species had an independent occupancy (ψBM CM = ψBM cm, SIF = 1) and exhibited a positive species interaction in detection probability (pBM

Abstract Background Early recurrence is the major cause of poor prognosis in hepatocellular carcinoma (HCC). Long non-coding RNAs (lncRNAs) are deeply involved in HCC prognosis. In this study, we aimed to establish a prognostic lncRNA signature for HCC early recurrence. Methods The lncRNA expression profile and corresponding clinical data were retrieved from total 299 HCC patients in TCGA database. LncRNA candidates correlated to early recurrence were selected by differentially expressed gene (DEG), univariate Cox regression and least absolute shrinkage and selection operator (LASSO) regression analyses. A 25-lncRNA prognostic signature was constructed according to receiver operating characteristic curve (ROC). Kaplan-Meier and multivariate Cox regression analyses were used to evaluate the performance of this signature. ROC and nomogram were used to evaluate the integrated models based on this signature with other independent clinical risk factors. Gene set enrichment analysis (GSEA) was used to reveal enriched gene sets in the high-risk group. Tumor infiltrating lymphocytes (TILs) levels were analyzed with single sample Gene Set Enrichment Analysis (ssGSEA). Immune therapy response prediction was performed with TIDE and SubMap. Chemotherapeutic response prediction was conducted by using Genomics of Drug Sensitivity in Cancer (GDSC) pharmacogenomics database. Results Compared to low-risk group, patients in high-risk group showed reduced disease-free survival (DFS) in the training (p

Perfluorobutanesulfonate (PFBS) is a ubiquitous pollutant in the aquatic environment, but its toxic effects and mechanisms on amphibian species remain largely unknown. In the present study, tadpoles (Lithobates catesbeianus) were exposed to various concentrations of PFBS (0, 1, 3, 10, and 30 ​μg/L) for 14 days, with the goal of unveiling the impairment of intestinal health. Histopathological examination showed that sub-lethal exposure of tadpoles to PFBS at concentrations as low as 3 ​μg/L could result in the injury of intestinal structures. In a clear concentration-dependent manner, the expressions of epithelial barrier components (i.e., Claudin 1 gene and tight junction protein 2) were significantly decreased in PFBS-exposed intestines, while the intestinal content of lipopolysaccharide (LPS) and transcriptions of downstream responsive genes (e.g., TLR4, MyD88, and NF-κB) were concurrently significantly increased by exposure to 3, 10, and 30 ​μg/L of PFBS. As a consequence, the number of eosinophils and expression of pro-inflammatory cytokines (e.g., IL-1β and TNF-α) were increased therein. Furthermore, PFBS exposure induced oxidative stress in intestinal tissues by increasing the level of reactive oxygen species (ROS) and suppressing antioxidant capacity. The transcriptional levels of CytoC and Bax genes as well as activities of caspase 9 and caspase 3 enzymes were remarkably increased, while the transcript abundance of Bcl-2 was down-regulated significantly after PFBS exposure, thereby favoring apoptosis in tadpole intestines. PFBS sub-lethal exposure also drove the composition of intestinal microbiota to a dysbiosis status. Correlation analysis further revealed that the relative abundance of members of the genus Bosea was positively related with the contents of LPS and IL-1β. Overall, the present study provides the first evidence for pronounced impacts of PFBS on amphibian intestinal ecology, highlighting the susceptibility of tadpoles to the environmental risks of PFBS.

In this work, a fluorophores-assisted excitation/emission matrix (EEM) fluorescence method was proposed to trace the origin of lily in the Chinese market. There are few active components in lilies that have fluorescent signals, and too few characteristic variables may lead to unsatisfactory accuracy in the subsequent classification. Therefore, three fluorophores, 2-Aminoethyl diphenylborinate (DPBA), o-Phthalaldehyde (OPA) and Rhodamine B (RB), were used to enrich the information of the fluorescent fingerprint of lily, which can improve the classification accuracy. The lily samples were characterized by using EEM fluorescence coupled with the alternating trilinear decomposition (ATLD) algorithm, which was able to extract information of various fluorophores in lily samples. Two chemical pattern recognition methods, principal component analysis-linear discriminant analysis (PCA-LDA) and partial least squares-discrimination analysis (PLS-DA), were used to model and trace the origin of different lilies. When the fluorophores were added, the accuracy of the test set and prediction set obtained by the classification model increased from 71.4% to 92.9% and 66.7% to 100%, respectively. The proposed method combined fluorophores-assisted EEM fluorescence with multi-way chemometric methods to extract comprehensive information on the samples, which provided a potential method for the origin traceability of traditional Chinese medicine.

Abstract Background & aims Transient regeneration–associated steatosis (TRAS) is a process of temporary hepatic lipid accumulation and is essential for liver regeneration by providing energy generated from fatty acid β‐oxidation, but the regulatory mechanism underlying TRAS remains unknown. Parkinsonism‐associated deglycase (Park7)/Dj1 is an important regulator involved in various liver diseases. In nonalcoholic fatty liver diseased mice, induced by a high‐fat diet, Park7 deficiency improves hepatic steatosis, but its role in liver regeneration remains unknown Methods Park7 knockout (Park7−/−), hepatocyte‐specific Park7 knockout (Park7△hep) and hepatocyte‐specific Park7‐Pten double knockout mice were subjected to 2/3 partial hepatectomy (PHx) Results Increased PARK7 expression was observed in the regenerating liver of mice at 36 and 48 h after PHx. Park7−/− and Park7△hep mice showed delayed liver regeneration and enhanced TRAS after PHx. PPARa, a key regulator of β‐oxidation, and carnitine palmitoyltransferase 1a (CPT1a), a rate‐limiting enzyme of β‐oxidation, had substantially decreased expression in the regenerating liver of Park7△hep mice. Increased phosphatase and tensin homolog (PTEN) expression was observed in the liver of Park7△hep mice, which might contribute to delayed liver regeneration in these mice because genomic depletion or pharmacological inhibition of PTEN restored the delayed liver regeneration by reversing the downregulation of PPARa and CPT1a and in turn accelerating the utilization of TRAS in the regenerating liver of Park7△hep mice Conclusion Park7/Dj1 is a novel regulator of PTEN‐dependent fatty acid β‐oxidation, and increasing Park7 expression might be a promising strategy to promote liver regeneration.

The prognosis of patients with cholangiocarcinoma (CCA) is closely related to both immune cell infiltration and mRNA expression. Therefore, we aimed at conducting multi-immune-related gene analyses to improve the prediction of CCA recurrence. Immune-related genes were selected from the Gene Expression Omnibus (GEO), The Cancer Genome Atlas (TCGA), and the Immunology Database and Analysis Portal (ImmPort). The least absolute shrinkage and selection operator (LASSO) regression model was used to establish the multi-gene model that was significantly correlated with the recurrence-free survival (RFS) in two test series. Furthermore, compared with single genes, clinical characteristics, tumor immune dysfunction and exclusion (TIDE), and tumor inflammation signature (TIS), the 8-immune-related differentially expressed genes (8-IRDEGs) signature had a better prediction value. Moreover, the high-risk subgroup had a lower density of B-cell, plasma, B-cell naïve, CD8+ T-cell, CD8+ T-cell naïve, and CD8+ T-cell memory infiltration, as well as more severe immunosuppression and higher mutation counts. In conclusion, the 8-IRDEGs signature was a promising biomarker for distinguishing the prognosis and the molecular and immune features of CCA, and could be beneficial to the individualized immunotherapy for CCA patients.

Abstract Background Systematic studies on the development and adaptation of hindlimb muscles in anura amphibians are rare. Here, we integrated analysis of transcriptome and histomorphological data for the hindlimb thigh muscle of Odorrana tormota (concave-eared torrent frog) at different developmental stages, to uncover the developmental traits of hindlimb thigh muscle from O. tormota and its adaptability to different life history stages. Results The development of hindlimb thigh muscle from O. tormota has the following characteristics. Before metamorphosis, myogenous cells proliferate and differentiate into myotubes, and form 11 muscle groups at G41; Primary myofibers and secondary myofibers appeared during metamorphosis; 11 muscle groups differentiated continuously to form myofibers, accompanied by myofibers hypertrophy after metamorphosis; During the growth process of O. tormota from G42 to G46, there were differences between the sexes in the muscle groups that differentiate into muscle fibers, indicating that there was sexual dimorphism in the hindlimb thigh muscles of O. tormota at the metamorphosis stages. Some genes and pathways related to growth, development, and movement ability of O. tormota at different developmental stages were obtained. In addition, some pathways associated with adaptation to metamorphosis and hibernation also were enriched. Furthermore, integrated analysis of the number of myofibers and transcriptome data suggested that myofibers of specific muscle groups in the hindlimbs may be degraded through lysosome and ubiquitin pathways to transform into energy metabolism and other energy-related substances to meet the physiological needs of hibernation. Conclusions These results provide further understanding the hindlimb thigh muscle development pattern of frogs and their adaption to life history stages.

Background & Aims: DJ-1 is universally expressed in various tissues and organs and is involved in the physiological processes in various liver diseases. However, the role of DJ-1 in liver ischemia-reperfusion (I/R) injury is largely unknown. Methods: In this study, we first examined the DJ-1 expression changes in the liver tissues of mice and clinical donor after hepatic I/R by both quantitative polymerase chain reaction and Western blotting assays. Then we investigated the role of DJ-1 in I/R injury by using a murine liver I/R model. Results: We demonstrated that DJ-1 down-regulation in both human and mouse liver tissues in response to I/R injury and Dj-1 deficiency in hepatocytes but not in myeloid cells could significantly ameliorate I/R induced liver injury and inflammatory responses. This hepatoprotective effect was dependent on enhanced autophagy in Dj-1 knockout mice, because inhibition of autophagy by 3-methyladenine and chloroquine could reverse the protective effect on hepatic I/R injury in Dj-1 knockout mice. Conclusions: Dj-1 deficiency in hepatocytes significantly enhanced mitochondrial accumulation and protein stability of PARKIN, which in turn promotes the onset of mitophagy resulting in elevated clearance of damaged mitochondria during I/R injury.

Despite the importance of the skin mucosal barrier and commensal microbiota for the health of amphibians, the potential of environmental contaminants to disrupt the skin mucosal barrier and microbiota have rarely been studied in toxicology. In this study, tadpoles (Lithobates catesbeianus) were exposed to 0, 0.5, and 2 μg/L of microcystin-leucine arginine (MC-LR) for 30 days to explore the impacts of environmentally realistic MC-LR concentrations on the physical skin barrier, immune barrier, commensal microbiota, and skin resistance to pathogenic bacterial invasion. MC-LR exposure significantly reduced the collagen fibrils in the dermis of skin tissues and down-regulated tight junction and stratum corneum-related gene transcriptions, suggesting the damage caused by MC-LR to the physical barrier of the skin. Increased skin eosinophils and upregulated transcriptions of inflammation-related genes in the exposed tadpoles underline the development of skin inflammation resulting from MC-LR exposure even at environmentally realistic concentrations. Comparative transcriptome and immunobiochemical analyses found that antimicrobial peptides (Brevinin-1PLc, Brevinin-2GHc, and Ranatuerin-2PLa) and lysozyme were down-regulated in the exposed groups, while complement, pattern recognition receptor, and specific immune processes were up-regulated. However, the content of endotoxin lipopolysaccharide produced by bacteria increased in a dose-dependent pattern. The disc diffusion test showed a reduced ability of skin supernatant to inhibit pathogenic bacteria in the exposed groups. Analysis of microbial 16 S rRNA gene by high-throughput sequencing revealed that MC-LR interfered with the abundance, composition, and diversity of the skin commensal microbiota, which favored the growth of pathogen-containing genera Rhodococcus, Acinetobacter, and Gordonibacter. In summary, the current study provides the first clues about the impact of MC-LR on the integrity and function of skin barrier of amphibians. These new toxicological evidences can facilitate a more comprehensive evaluation of the ecological risk of MC-LR to amphibians.

In arid and semiarid regions with water shortage, forestry development is limited by water availability. Understanding how tree sap flow responds to water stress and microclimatic variables is essential for the management of trees and the understanding of the eco-physiological properties of trees in arid areas. In the city of Tianjin in northern China, we measured the sap flow of Fraxinus pennsylvanica, a widely distributed urban greening tree species in semiarid regions of China. We measured the sap flow in four F. pennsylvanica trees over 6 months (April–September 2021), using a thermal diffusion probe method, and simultaneously monitored microclimatic variables and soil moisture. Results indicated that high nighttime sap flow velocity might be produced under the water stress condition. In addition, the nighttime sap flow velocity under the water stress condition was more susceptible to the combined effects of meteorological factors at night. The daytime sap flow velocity exerted a highly significant positive effect on the nighttime sap flow velocity during the whole research period, and the model fit was higher in the early growing season than that in the late growing season (early growing season: R2 = 0.51, P < 0.01; late growing season: R2 = 0.36, P < 0.01). Vapor pressure deficit had a positive effect on daytime sap flow. However, net vapor pressure deficit restrained daytime sap flow velocity when the intercorrelation between the microclimatic variables was removed. Our study highlights that drought areas perhaps have higher nighttime sap flow and that more emphasis should be placed on nighttime sap flow and the response of nighttime sap flow to microclimatic variables. In addition, the influence of other microclimatic variables on vapor pressure deficit needs to be considered when analyzing the relationship between daytime sap flow and vapor pressure deficit. An increase in net VPD can suppress the daytime sap flow.

Enhanced nucleoside metabolism is one of the hallmarks of cancer. Uridine-cytidine kinase 2 (UCK2) is a rate-limiting enzyme of the pyrimidine salvage synthesis pathway to phosphorylate uridine and cytidine to uridine monophosphate (UMP) and cytidine monophosphate (CMP), respectively. Recent studies have shown that UCK2 is overexpressed in many types of solid and hematopoietic cancers, closely associates with poor prognosis, and promotes cell proliferation and migration in lung cancer and HCCs. Although UCK2 is thought to catalyze sufficient nucleotide building blocks to support the rapid proliferation of tumor cells, we and other groups have recently demonstrated that UCK2 may play a tumor-promoting role in a catalytic independent manner by activating oncogenic signaling pathways, such as STAT3 and EGFR-AKT. By harnessing the catalytic activity of UCK2, several cytotoxic ribonucleoside analogs, such as TAS-106 and RX-3117, have been developed for UCK2-mediated cancer chemotherapy. Moreover, we have demonstrated that the concurrent targeting of the catalytic dependent and independent features of UCK2 could synergistically inhibit tumor growth. These findings suggest that UCK2 may serve as a potential therapeutic target for cancer treatment. In this mini-review, we introduced the genomic localization and protein structure of UCK2, described the role of UCK2 in tumor development, discussed the application of UCK2 in anti-tumor treatment, and proposed concurrent targeting of the catalytic and non-catalytic roles of UCK2 as a potential therapeutic strategy for cancer treatment.

Although the Chinese government has conducted much work in recent years to reduce land-based pollutant discharge, eutrophication continues to occur frequently in many rivers, estuaries, and coastal waters. This may indicate that sediment is a major source rather than a sink for nitrogen (N) and phosphorus (P). To clarify the endogenous mechanisms of eutrophication in coastal river basins, the eutrophication status, physicochemical properties, and bacterial parameters of overlying waters and sediments in the catchment (CA), estuarine (EA), and offshore (OA) areas in the Duliujian River Basin of Bohai Bay were investigated. The results showed that the eutrophication index (EI) of CA, EA, and OA were 62.71, 57.86, and 36.51, respectively. The EI was more sensitive to increases in P (slope = 3.887) than to increases in N (slope = 0.734) of the overlying water, indicating that P is the main factor driving eutrophication in the coastal river basin. However, a nonlinear relationship was found between P in sediments and overlying waters, suggesting that bacterial mediation may occur during P release. As speculated in this study, P in the overlying water increased more quickly than N with increasing bacterial diversity and metabolic abundance, indicating that the allometric release of N and P mediated by bacteria increases the risk of eutrophication. Redundancy analysis showed that organic matter and total N in sediment have positive effects on bacterial communities, which explains 21.8% and 23.7% variation in bacterial diversity, and explains 31.3% and 7.1% variation in bacterial metabolism. This also suggests that the accumulation of N in the sediment promotes the release of P and further aggravates the eutrophication of water. Therefore, simultaneous control of N and P is necessary to control water eutrophication in coastal river basins.

Sennoside A (SA) is a natural dianthrone glycoside mainly from medicinal plants of Senna and Rhubarb, and used as a folk traditional irritant laxative and slimming health food. Accumulating evidences suggest that SA possesses numerous pharmacological properties, such as laxative, anti-obesity, hypoglycemic, hepatoprotective, anti-fibrotic, anti-inflammatory, anti-tumor, anti-bacterial, anti-fungal, anti-viral, and anti-neurodegenerative activities. These pharmacological effects lay the foundation for its potential application in treating a variety of diseases. However, numerous published studies suggest that a long-term use of SA in large doses may have some adverse effects, including the occurrence of melanosis coli and carcinogenesis of colon cancer, thereby limiting its clinical use. It remains to be established whether SA or its metabolites are responsible for the pharmacological and toxicity effects. In this review, the latest advances in the pharmacology, toxicology, and metabolism of SA were summarizedbased on its biological characteristics and mechanism.

In this paper, we propose a spectrum sensing algorithm based on the Jones vector covariance matrix (JCM) and AlexNet model, i.e., the JCM-AlexNet algorithm, by taking advantage of the different state characteristics of the signal and noise in the polarization domain. We use the AlexNet model, which is good at extracting matrix features, as the classification model and use the Jones vector, which characterizes the polarization state, to calculate its covariance matrix and convert it into an image and then use it as the input to the AlexNet model. Then, we calculate the likelihood ratio test statistic (AlexNet-LRT) based on the output of the model to achieve the classification of the signal and noise. The simulation analysis shows that the JCM-AlexNet algorithm performs better than the conventional polarization detection (PSD) algorithm and the other three (LeNet5, long short-term memory (LSTM), multilayer perceptron (MLP)) excellent deep-learning-based spectrum sensing algorithms for different signal-to-noise ratios and different false alarm probabilities.

The golden tide, a large biomass bloom of the brown macroalgae Sargassum horneri, occurs yearly in the Yellow Sea, where it causes enormous economic and ecologic losses. To investigate the response of S. horneri to global warming and eutrophication, S. horneri was cultured under six conditions of varying temperature combinations (20 and 24 °C) and nitrogen levels (5, 30, and 300 μM). The growth, photosynthetic performance, pigment content, and contents of soluble protein were assessed. The growth of S. horneri followed an increasing trend with increasing N concentration at ambient temperature. Elevated temperatures had an inhibitory effect on growth and photosynthesis in S. horneri, which was further enhanced by eutrophication. This suggests that in the globally warming environment of the future, eutrophication may reduce the frequency and scale of gold tide outbreaks during the hot season.

Background: Acute liver failure (ALF) and acute-on-chronic liver failure (ACLF) are characterized by systemic inflammation and high mortality, but there is no effective clinical treatment. As a classic traditional Chinese medicine (TCM) formula, MaHuang-LianQiao-ChiXiaoDou decoction (MHLQD) has been used clinically for centuries to treat liver diseases. Methods: The LPS/D−GalN-induced ALF mice model and the CCl4+LPS/D−GalN-induced ACLF mice model were used to observe the therapeutic effects of MHLQD on mice mortality, hepatocytes death, liver injury, and immune responses. Results: MHLQD treatment significantly improved mice mortality. Liver injury and systemic and hepatic immune responses were also ameliorated after MHLQD treatment. Mechanistically, proteomic changes in MHLQD-treated liver tissues were analyzed and the result showed that the thrombogenic von Willebrand factor (VWF) was significantly inhibited in MHLQD-treated ALF and ACLF models. Histological staining and western blotting confirmed that VWF/RAP1B/ITGB3 signaling was suppressed in MHLQD-treated ALF and ACLF models. Furthermore, mice treated with the VWF inhibitor ADAMTS13 showed a reduced therapeutic effect from MHLQD treatment. Conclusions: Our study indicated that MHLQD is an effective herbal formula for the treatment of ALF and ACLF, which might be attributed to the protection of hepatocytes from death via VWF/RAP1B/ITGB3 signaling.

Background: Gliomas are the most common intracranial malignant neoplasms and have high recurrence and mortality rates. Recent literatures have reported that centromere protein N (CENPN) participates in tumor development. However, the clinicopathologic significance and biological functions of CENPN in glioma are still unclear.Methods: Clinicopathologic data and gene expression profiles of glioma cases downloaded from The Cancer Genome Atlas (TCGA) and Chinese Glioma Genome Atlas (CGGA) databases were utilized to determine the associations between the expression of CENPN and clinical features of glioma. Kaplan-Meier and ROC curves were plotted for prognostic analysis. Gene set enrichment analysis (GSEA) and single sample gene set enrichment analysis (ssGSEA) were applied to identify immune-related functions and pathways associated with CENPN’ differential expression. In vitro experiments were conducted to investigate the impacts of CENPN on human glioma cells.Results: Elevated CENPN expression was associated with unfavorable clinical variables of glioma patients, which was validated in clinical specimens obtained from our institution by immunohistochemical staining (IHC). The GSEA and ssGSEA results revealed that CENPN expression was strongly correlated with inflammatory activities, immune-related signaling pathways and the infiltration of immune cells. Cell experiments showed that CENPN deficiency impaired cell proliferation, migration and invasion ability and increased glioma apoptosis.Conclusion: CENPN could be a promising therapeutic target for glioma.

Abstract Background A study was conducted to evaluate the choroidal thickness (CT) and retinal thickness (RT) in paediatric patients with hypoalbuminaemia caused by nephrotic syndrome (NS). We also studied the correlation between the subfoveal choroidal thickness (SFCT) and serum protein concentration. Methods This was a cross-sectional study. Fifty-one paediatric patients with hypoalbuminaemia caused by NS and 41 normal subjects were included in the study. Enhanced depth imaging optical coherence tomography (EDI-OCT) was performed to measure the RT and CT. The RT and CT were measured manually at intervals of 0.5 mm along a horizontal line through the macular fovea between 2.5 mm nasal and 2.5 mm temporal to the fovea. Clinical data including measurements of serum proteins were obtained. Results The mean RTs at the T2.5, T2, N1.5, N2, and N2.5 locations and the average macular horizontal RT were slightly greater in the NS group than those in the control group. The mean CTs at all locations were significantly greater in the NS group than those in the control group; the difference was most significant at the fovea (373.8 ± 74.9 μm vs. 280.2 ± 57.1; p

Background & Aims: Chemokine-mediated immune cell recruitment plays pivotal roles in liver inflammation. C-C motif chemokine ligand 5 (CCL5) has been shown to be responsible for the recruitment of monocytes/macrophages and has been implicated in various liver diseases, including nonalcoholic fatty liver disease, fibrosis, and hepatocellular carcinoma. Previous studies have also shown that inhibition of CCL5 appears to be a promising therapeutic approach for several chronic liver diseases. However, whether blocking CCL5 could benefit immune cell–mediated hepatitis remains largely elusive. Methods: By adopting a specific agonist, alpha-galactosylceramide (α-Galcer), of invariant natural killer T cells (iNKTs), we investigated the function and mechanism of CCL5 in the iNKT induced murine hepatitis model. Results: We found significantly increased CCL5 expression in α-Galcer–induced hepatitis murine model. Such an increase in CCL5 is mainly enriched in non-parenchymal cells such as macrophages and iNKTs but not in hepatocytes. Surprisingly, CCL5 blockage by genetic deletion of Ccl5 does not affect the α-Galcer–induced iNKT activation but greatly worsens α-Galcer–induced liver injury accompanied by an increased hepatic neutrophil infiltration. Mechanistically, we demonstrated that greater neutrophil accumulation in the liver is responsible for the enhanced liver injury in Ccl5-/- mice. Such an increased hepatic neutrophil infiltration is mainly caused by an enhanced CXCL1-CXCR2 signal in Ccl5-/- mice. Therapeutically, either antibody-mediated neutrophil depletion or a CXCR2 antagonist, SB225002, mediated CXCR2 signaling blockage significantly ameliorated α-Galcer–induced liver injury in Ccl5-/- mice. Conclusions: Our present study demonstrates that (1) α-Galcer–induced murine hepatitis could greatly induce CCL5 production in macrophages and iNKT cells; (2) loss of CCL5 could enhance CXCL1 expression in hepatocytes and activate CXCL1-CXCR2 axis in neutrophils to augment their hepatic infiltration; and (3) neutrophil depletion or blockage of CXCL1-CXCR2 axis greatly improves α-Galcer–induced liver injury in Ccl5-/- mice. This study suggests that clinical utilization of CCL5 blockage may compensatorily induce the activation of other chemokine pathways to enhance neutrophil recruitment and liver injury in hepatitis. Keywords: CCL5, Invariant NKT, Neutrophils, CXCL1, CXCR2

The vast majority of existing sub-Nyquist sampling wideband spectrum sensing (WSS) methods default to a sparse spectrum. However, research data suggests that in the near future, the wideband spectrum will no longer be sparse. This article proposes a sub-Nyquist sampling WSS algorithm that can adapt well to non-sparse spectrum scenarios. The algorithm continues to implement the idea of our previously proposed “no reconstruction (NoR) of spectrum” algorithm, thus having low computational complexity. The new one is actually an advanced version of the NoR algorithm, so it is called AdNoR. The key to its advancement lies in the establishment of a folded time-frequency (TF) spectrum model with the same special structure as in the fold spectrum model of the NoR algorithm. For this purpose, we have designed a comprehensive sampling technique which consists of multicoset sampling, digital fractional delay, and TF transform. It is verified by simulation that the AdNoR algorithm maintains a good sensing performance with low computational complexity in the non-sparse scenario.

Microcystin-LR (MC-LR) is widely present in waters around the world, but its potential toxic effects and mechanisms on amphibian gills remain unknown. In the present study, tadpoles (Lithobates catesbeianus) were exposed to environmentally realistic concentrations of 0.5, 2 μg/L MC-LR, and 0 μg/L MC-LR (Control) for 30 days with the objective to unveil the impairment of gill health. The lysozyme was downregulated, while pattern recognition receptors and complement and adaptive immune processes were upregulated and the ability of gill supernatant to inhibit pathogenic bacteria decreased in the 0.5 and 2 μg/L MC-LR groups. The transcriptions of epithelial barrier components (e.g., CLDN1) were significantly decreased in MC-LR-exposed gills, while the gill content of lipopolysaccharide (LPS) endotoxins and the transcriptions of downstream responsive genes (e.g., TLR4 and NF-κB) were concurrently increased. In addition, the number of eosinophils and the expression of pro-inflammatory cytokines (e.g., IL-1β and TNF-α) were increased. These results imply that exposure of tadpoles to low environmentally concentrations of MC-LR leads to inflammation, increased permeability, and a reduced ability to inhibit pathogenic bacteria. The epithelial cells of inner gill filaments increased and transcriptions of hypoxic stress genes (e.g., HIF-1α, FLT1, and SERPINE1) were upregulated within the exposed group. As a consequence, exposure to MC-LR may lead to hypoxic stress. MC-LR exposure also drove gill microbiota to a dysbiosis. The relative abundance of Elizabethkingia was positively correlated with content of LPS and transcriptions of NF-κB and TNF-α. Overall, this study presents the first evidence about the pronounced impacts of MC-LR exposure on gills of amphibians, highlighting the susceptibility of early developing tadpoles to the environmental risks of MC-LR.

Hepatocellular carcinoma (HCC) is one of the leading causes of cancer-related death worldwide. Due to the lack of potent diagnosis and prognosis biomarkers and effective therapeutic targets, the overall prognosis of survival is poor in HCC patients. Circular RNAs (circRNAs) are a class of novel endogenous non-coding RNAs with covalently closed loop structures and implicated in diverse physiological processes and pathological diseases. Recent studies have demonstrated the involvement of circRNAs in HCC diagnosis, prognosis, development, and drug resistance, suggesting that circRNAs may be a class of novel targets for improving HCC diagnosis, prognosis, and treatments. In fact, some artificial circRNAs have been engineered and showed their therapeutic potential in treating HCV infection and gastric cancer. In this review, we introduce the potential of circRNAs as biomarkers for HCC diagnosis and prognosis, as therapeutic targets for HCC treatments and discuss the challenges in circRNA research and chances of circRNA application.

Sennoside A (SA) is a bioactive component of Rheum officinale Baill. with an activity of irritant laxative, which has been reported to possess therapeutic potential in various diseases or conditions including obesity, insulin resistance, liver steatosis, prostate cancer and pancreatic cancer progression. However, whether SA has therapeutic potential in hepatocellular carcinoma (HCC) treatment remains elusive. In this study, we treated two HCC cell lines, HepG2 and SMMC-7721 with SA and found that SA selectively inhibited the growth of HCC cells by proliferation assay. SA has a good inhibitory effect on proliferation of HepG2 cells in a concentration dependent manner, but there was no effect on SMMC-7721 cells. Then we conducted transwell assays and transcriptome analysis in HCC cells and examined the effects of SA on HCC in vivo. The results showed that SA significantly inhibited the migration and invasion of HCC. Comparison of RNA-seq transcriptome profiles from control groups and SA-treated groups identified 171 and 264 differentially expressed genes (DEGs) in HepG2 and SMMC-7721 cells respectively, in which includes 2 overlapping up-regulated DEGs and 12 overlapping down-regulated DEGs between HepG2 and SMMC-7721 cells. The qPCR were applied to investigate the transcriptional level of 9 overlapping down-regulated DEGs related to cancer metastasis, and the results were consistent with RNA-seq data. The dominate pathways including Wnt signaling pathway, TNF signaling pathway, VEGF signaling pathway, and NF-κB signaling pathway were strongly inhibited by SA, which are involved in regulating cancer metastasis. Finally, we confirmed that the downregulation of KRT7 and KRT81 could inhibit HCC metastasis. This study has provided new insight into the understanding of the inhibitory effects and potential targets of SA on the metastasis of HCC.

Because of the requirement of the mechanical properties of the damaged surrounding granite rock and the existence of the fracture water in hydraulic fracturing engineering, the strength of granite is related to the fabric, and the roughness of the section is also related to the liquid flow rate, a method of roughly determining the strength of the specimen by observing the failure mode of rock is needed. Considering that the physical and mechanical properties of granite are obviously affected by the fabric, the discrete element numerical simulation method was used to reconstruct the granite fabric based on the spatial correlation function to simulate the splitting experiment to investigate the failure mode of the specimen. The relationship between strength, the fractal value of cross-section, and the fabric was analyzed, which was verified through experiment. The results show that (1) the Voronoi GBM model with spatial correlation function of the discrete element can effectively simulate the controllable granite fabric and carry out micromechanical analysis. (2) The strength of the granite specimen and the fractal value of the cross-section have an obvious linear relationship with the fabric; besides, there is also a certain linear relationship between the strength of the specimen and the fractal value, which is influenced by granite fabric. (3) The predicted strength of the specimen according to the fractal value of the section is in good agreement with the actual strength with the error rate of 30%. In a word, this method can predict the strength of the specimen through the failure section and analyze the hydraulic fracture section and water pressure.

CUX1 belongs to the homeodomain transcription factor family and is evolutionarily and functionally conserved from Drosophila to humans. In addition to the involvement in various physiological events including tissue development, cell proliferation, differentiation and migration, and DNA damage response, CUX1 has been implicated in tumorigenesis. Interestingly, CUX1 has been recently recognized as a haploinsufficient tumor suppressor, which is paradoxically overexpressed in tumor cells. While loss of heterozygosity and/or mutations of CUX1 have been frequently detected in many types of cancers, genomic amplification, and overexpression of CUX1 have also been reported in cancer tissues and are correlated with higher tumor grade and poor prognosis. Therefore, deciphering the roles of different CUX1 isoforms and in different tumor stages is required to establish a CUX1-based therapeutic strategy for cancer treatment.

Long noncoding RNAs (lncRNAs) are important regulatory factors in multiple biological processes, and several lncRNAs are known to respond to insecticides. However, the lncRNA functions that are associated with terpinen-4-ol resistance in the red flour beetle (Tribolium castaneum) have not yet been identified. In this study, we determined the differentially transcribed lncRNAs between fumigated and control experimental groups. In the six libraries that underwent RNA sequencing, 34,546 transcripts were identified, including 8267 novel lncRNAs, 4155 novel mRNAs, 1151 known lncRNAs, and 20,973 known mRNAs. Among these, we found that the expression of 1858 mRNAs and 1663 lncRNAs was significantly different in the fumigated group compared with the control group. Among the differentially transcribed lncRNAs, 453 were up-regulated and 1210 were down-regulated lncRNAs. In addition, we identified the regulatory function targets of the lncRNAs. Functionally, all lncRNAs and target genes associated with terpinen-4-ol metabolism were enriched in several metabolic pathways, like the ATP-binding cassette transporter, pentose interconversion, and glucuronate interconversion. To the best of our knowledge, this study represents the first global identification of lncRNAs and their potential association with terpinen-4-ol metabolism in the red flour beetle. These results will provide reference information for studies on the resistance to terpinen-4-ol and other essential oil compounds and chemical pesticides, as well as an understanding of other biological processes in T. castaneum.

This paper presents spectrum sensing as a classification problem, and uses a spectrum-sensing algorithm based on a signal covariance matrix and long short-term memory network (CM-LSTM). We jointly exploited the spatial cross-correlation of multiple signals received by the antenna array and the temporal autocorrelation of single signals; we used the long short-term memory network (LSTM), which is good at extracting temporal correlation features, as the classification model; we then input the covariance matrix of the signals received by the array into the LSTM classification model to achieve the fusion learning of spatial correlation features and temporal correlation features of the signals, thus significantly improving the performance of spectrum sensing. Simulation analysis shows that the CM-LSTM-based spectrum-sensing algorithm shows better performance compared with support vector machine (SVM), gradient boosting machine (GBM), random forest (RF), and energy detection (ED) algorithm-based spectrum-sensing algorithms for different signal-to-noise ratios (SNRs) and different numbers of secondary users (SUs). Among them, SVM is a classical machine-learning algorithm, GBM and RF are two integrated learning methods with better generalization capability, and ED is a classical, traditional, and spectrum-sensing algorithm.

Understanding how the direct and indirect effects of climate change may affect species distributions is a key topic in ecology. We used maximum entropy models to explore the distribution of two species of shrews (Chodsigoa hypsibia and Anourosorex squamipes) in China and analyzed the main environmental factors affecting their current distribution and potential distribution changes under two future climate scenarios. The results showed that the major environmental factors determining the current distribution of C. hypsibia were the mean temperature of the coldest quarter (contributing 47.4%), annual mean temperature (contributing 24.7%), precipitation of the driest quarter (contributing 21.1%) and isothermality (contributing 6%). Annual precipitation (contributing 42.9%), precipitation of the driest month (contributing 28.1%), annual mean temperature (contributing 14.8%) and temperature seasonality (contributing 12.6%) had the highest contributions to the distribution of A. squamipes. Under future climate scenarios, the suitable habitat range of C. hypsibia increased while that of A. squamipes decreased. These findings demonstrate that different small mammal species respond differently to climate change.

Reactive oxygen species (ROS) and immune response play critical roles in the progression of liver diseases. DJ-1, also known as Parkinson disease 7 (Park7), is extensively expressed in cells and tissues, where it governs numerous biological functions including chaperone activity, protease function, transcriptional and mitochondrial regulation, and ROS modulation. Moreover, we have established that DJ-1 plays a critical role in initiating an inflammatory response by modulating ROS generation. Therefore, DJ-1 may play an important role in the progression of liver diseases by modulating ROS and the immune response. Recently, we have shown that DJ-1 deficiency negatively regulates proliferation of hepatic progenitor cells (HPCs) by impairing the formation of HPC-associated fibrosis and inflammatory niches. Deficiency of DJ-1 ameliorates liver fibrosis by inhibiting hepatic ROS production and inflammation; moreover, in a classic diethylnitrosamine (DEN)-mediated hepatocellular carcinoma (HCC) mouse model, deletion of DJ-1 ameliorates tumorigenesis and HCC cell proliferation by regulating hepatic inflammation and reducing the activity of the interleukin 6/signal transducer and activator of transcription 3 (IL-6/STAT3) signaling pathway. Taken together, these data suggest a critical function for, and therapeutic value of, DJ-1 in treatment of liver diseases. The aim of this review is to summarize these functions and the underlying molecular mechanisms of DJ-1 in liver diseases, and to highlight the potential therapeutic value and future research direction of DJ-1 in liver diseases. Keywords: DJ-1, Liver disease, Oxidative stress, Reactive oxygen species (ROS), Immune response

More degrees of freedom not only enable multiphase drives to be fault-tolerant but also allow non-sinusoidal electromotive forces (NS-EMFs) in high-quality vector control. NS-EMFs lead to lower costs of design and manufacturing of electrical machines. However, the presence of multi-harmonics in NS-EMFs possibly generates pulsating torque in both healthy and faulty conditions of multiphase drives. To facilitate the use of NS-EMFs, this two-part study proposes control schemes to adaptively improve torque quality of multiphase drives in dealing with multi-harmonics of NS-EMFs. The proposed schemes are based on a simple but effective type of artificial intelligence, Adaptive Linear Neuron (Adaline). The knowledge of multiphase drives including the harmonic ranks of NS-EMFs and the rotor position is exploited to design the online-trained optimal Adalines. The first part of this study is to propose a control scheme using an Adaline for healthy mode with high-quality torque regardless of numerous harmonics in NS-EMFs. The second part of this study introduces a control scheme using another Adaline for open-circuit faults. The proposed schemes are numerically and experimentally validated on a seven-phase permanent magnet synchronous machine (PMSM) possessing a high total harmonic distortion (THD = 38%) of NS-EMFs.

Fault tolerance has been known as one of the main advantages of multiphase drives. When an open-circuit fault happens, smooth torque can be obtained without any additional hardware. However, a reconfiguration strategy is required to determine new reference currents. Despite advantages of non-sinusoidal electromotive forces (NS-EMFs) such as high torque density, multi-harmonics existing in NS-EMFs cause more challenges for control, especially under faulty conditions. Therefore, to guarantee high-quality vector control of multiphase drives with multi-harmonic NS-EMFs, this two-part study proposes control schemes using adaptive linear neurons (Adalines) to adaptively eliminate torque ripples. The proposed simple Adalines are efficient because of taking advantage of the knowledge of rotor position and of torque harmonic rank induced by the NS-EMFs. The control scheme using an Adaline for healthy mode was described in part I of this study. In this second part, the control scheme using another Adaline for an open-circuit operation, under the impacts of multi-harmonics in NS-EMFs, is proposed. Notably, smooth torque and similar copper losses in the remaining healthy phases can be obtained. Experimental tests are carried out on a seven-phase permanent magnet synchronous machine (PMSM) with a high total harmonic distortion (THD = 38%) of NS-EMFs. A demonstration video is provided with this paper.

Biologists have discovered a kind of weakly electric fish that identifies its prey by using active electrolocation in virtual darkness. In this study, we built an underwater active electrolocation system platform designed to investigate the biological mechanism allowing these fish to distinguish objects and determine how the amplitude information-frequency characteristic (AIFC) response are affected by the geometric characteristics of target objects in the active electrolocation system. We used a single-frequency sinusoidal signal to scan metal objects in different orientations and observed the amplitude information response variation of the disturbed detection signal. The detection frequency dead zone (DFDZ) and the frequency inflection point (FIP) were used to characterize the variation. In addition, we repeated the experiments after replacing the metal objects with objects of different materials and geometric characteristics to summarize the general laws. Our results showed that the FIP value of the detection signal was lowest when the object was detected in the orientation of its corner and highest when the object was detected in the orientation of its surface. The geometrical characteristics of metal objects in different orientations have a certain influence on the amplitude of the detection signal. Article Highlights: (1) The general law between the shape of metal probed objects, and electric field detection signal was found and summarized. (2) We used a single-frequency sinusoidal signal to scan regular metal probed objects, and it was found that the frequency inflection point (FIP) of the metal probed objects edge was the highest, whereas that of the corner was the lowest. (3) The shape of a metal object can be recognized by scanning regular metal objects with an electric field signal.