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Abstract Background Improving feed efficiency (FE) is one of the main objectives in broiler breeding. It is difficult to directly measure FE traits, and breeders hence have been trying to identify biomarkers for the indirect selection and improvement of FE traits. Metabolome is the "bridge" between genome and phenome. The metabolites may potentially account for more of the phenotypic variation and can suitably serve as biomarkers for selecting FE traits. This study aimed to identify plasma metabolite markers for selecting high-FE broilers. A total of 441 birds from Northeast Agricultural University broiler lines divergently selected for abdominal fat content were used to analyze plasma metabolome and estimate the genetic parameters of differentially expressed metabolites. Results The results identified 124 differentially expressed plasma metabolites (P

Rice, a crucial staple in China, is cultivated through various techniques, including seedling transplanting, dry direct seeding, and film mulching. Despite its significance, rice production is a considerable environmental burden. Using a life cycle assessment (LCA) methodology, this study aimed to evaluate the environmental impacts of four rice cultivation methods (transplanting rice, dry direct-seeding rice, dry direct-seeding rice with polyethylene film (PE), and dry direct-seeding rice with biodegradable film) in Northeast China. The results indicate that the magnitude of environmental impacts among treatments was consistent across years. The potential values of all environmental impacts of the four different cultivation methods of rice in the 2021 field trial were smaller than the results of the same cultivation method of rice system in the 2022 field trial. Among the four rice cultivation methods, the consumption of energy showed inconsistency over the two years, with the highest energy consumption in the first year being for dry seeding with PE film and in the second year for dry seeding without film. Additionally, transplanting exhibited the highest impact on water resource consumption and climate change. Dry direct-seeding rice displayed the highest eutrophication and ecotoxicity. Dry direct-seeding rice with a biodegradable film had the least impact in terms of acidification. Moreover, dry direct-seeding rice with a biodegradable film minimized water consumption and greenhouse gas emissions without compromising yield.

In the realm of agriculture, biodegradable films are emerging as a promising substitute for traditional polyethylene (PE) films. Despite their potential, there has been a notable lack of extensive research on their effectiveness in the context of dry direct-seeded rice cultivation. Addressing this gap, a comprehensive biennial study was conducted in the northeastern regions of China, focusing on the ‘Baonong 5’ rice variety. This study meticulously compared three distinct cultivation methods: (1) employing biodegradable film mulching, (2) using conventional plastic film mulching, and (3) cultivating without any mulch. The findings revealed that biodegradable film mulching significantly enhanced soil moisture control, increased leaf area, and improved rice yield and water utilization efficiency (p < 0.05) compared to the plots without mulch. Notably, there was no marked difference in outcomes between the plastic film mulching and the unmulched plots. This research underscores the profound benefits of biodegradable film in rice cultivation, particularly from an environmental sustainability perspective. This innovative method not only boosts agricultural productivity but also addresses critical environmental challenges like climate change and water conservation. The application of biodegradable mulch has proven to be remarkably effective in improving irrigation efficiency and crop water conservation, leading to enhanced rice development and higher yields. The study recorded a substantial increase in water productivity—30% in 2021 and between 52.85% and 60% in 2022—compared to traditional cultivation practices. Furthermore, the use of biodegradable mulch resulted in significantly higher rice yields than the non-mulched plots, thus contributing to increased profitability. Such methods not only yield higher crop outputs but also mitigate environmental issues like water pollution and help alleviate prevalent water shortages in rice farming.

Rapid growth is one of the most important economic traits in broiler breeding programs. Identifying markers and genes for growth traits may not only benefit marker-assisted selection (MAS)/genomic selection (GS) but also provide important information for understanding the genetic architecture of growth traits in broilers. In the present study, an F2 resource population derived from a cross between the broiler and Baier yellow chicken (a Chinese local breed) was used and body weights from 1 to 12 weeks of age [body weight (BW) 1–BW12)] were measured. A total of 519 F2 birds were genome re-sequenced, and a combination of genome-wide association study (GWAS) and selective sweep analysis was carried out to characterize the genetic architecture affecting chicken body weight comprehensively. As a result, 1,539 SNPs with significant effects on body weights at different weeks of age were identified using a genome-wide efficient mixed-model association (GEMMA) package. These SNPs were distributed on chromosomes 1 and 4. Besides, windows under selection identified for BW1–BW12 varied from 1,581 to 2,265. A total of 42 genes were also identified with significant effects on BW1–BW12 based on both GWAS and selective sweep analysis. Among these genes, diacylglycerol kinase eta (DGKH), deleted in lymphocytic leukemia (DLEU7), forkhead box O17 (FOXO1), karyopherin subunit alpha 3 (KPNA3), calcium binding protein 39 like (CAB39L), potassium voltage-gated channel interacting protein 4 (KCNIP4), and slit guidance ligand 2 (SLIT2) were considered as important genes for broiler growth based on their basic functions. The results of this study may supply important information for understanding the genetic architecture of growth traits in broilers.

Abstract KLF3 is a member of the Kruppel‐like factor (KLF) family of transcription factors, and plays an important role in several biological processes, including adipogenesis, erythropoiesis and B‐cell development. The purposes of this study are to search for polymorphisms of KLF3 coding region and to provide functional evidence for abdominal fat in chickens. A total of 168 SNPs in KLF3 coding region were detected in a unique chicken population, the Northeast Agricultural University broiler lines divergently selected for abdominal fat content (NEAUHLF). Of which three single nucleotide polymorphisms (g.3452T > C, g.8663A > G and g.10751G > A) were significantly correlated with abdominal fat weight (AFW) and abdominal fat percentage (AFP) of 329 birds from the 19th generation of NEAUHLF (FDR A (p A is a functional variant that potential would be applied to marker‐assisted selection (MAS) for selective breeding programme.

This study aims to identify molecular marker loci that could be applied in broiler breeding programs. In this study, we used public databases to locate the Transcription factor 21 (TCF21) gene that affected the economically important traits in broilers. Ten single nucleotide polymorphisms were detected in the TCF21 gene by monoclonal sequencing. The polymorphisms of these 10 SNPs in the TCF21 gene were significantly associated (p < 0.05) with multiple growth and body composition traits. Furthermore, the TT genotype of g.-911T>G was identified to significantly increase the heart weight trait without affecting the negative traits, such as abdominal fat and reproduction by multiple methods. Thus, it was speculated that the g.-911T>G identified in the TCF21 gene might be used in marker-assisted selection in the broiler breeding program.