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Abstract Background Intervertebral disc degeneration (IDD) is the main pathogenesis of low back pain. MicroRNAs (miRNAs) have been found to exert regulatory function in IDD. This study aimed to investigate the effect and potential mechanism of miR-96-5p in IDD. Methods In vitro cell model of IDD was established by treating human nucleus pulposus cells (HNPCs) with interleukin-1β (IL-1β). The level of peroxisome proliferator-activated receptor γ (PPARγ) was examined in the IDD cell model by Western blot and quantification real-time reverse transcription-polymerase chain reaction (qRT-PCR). The expression level of miR-96-5p was detected by RT-qPCR. Effects of PPARγ or/and PPARγ agonist on inflammatory factors, extracellular matrix (ECM), apoptosis, and nuclear factor-kappaB (NF-κB) nuclear translocation were examined through enzyme-linked immunosorbent assay (ELISA), Western blot, flow cytometry assay, and immunofluorescence staining. The Starbase database and dual luciferase reporter assay were used to predict and validate the targeting relationship between miR-96-5p and PPARγ, and rescue assay was performed to gain insight into the role of miR-96-5p on IDD through PPARγ/NF-κB signaling. Results PPARγ expression reduced with concentration and time under IL-1β stimulation, while miR-96-5p expression showed the reverse trend (P

Abstract Cost‐effective non‐noble metal‐based catalysts for selective hydrogenation with excellent activity, selectivity, and durability are still the holy grail. Herein, an oxygen‐doped carbon (OC) chainmail encapsulated dilute Cu–Ni alloy is developed by simple pyrolysis of Cu/Ni‐metal–organic framework. The CuNi0.05@OC catalyst displays superior performance for atmospheric pressure transfer hydrogenation of p‐chloronitrobenzene and p‐nitrophenol, and for hydrogenation of furfural, all in water and with exceptional durability. Comprehensive characterizations confirm the close interactions between the diluted Ni sites, the base Cu, and optimized three‐layered graphene chainmail. Theoretical calculations demonstrate that the properly tuned lattice strain and Schottky junction can adjust electron density to facilitate specific adsorption on the active centers, thus enhancing the catalytic activity and selectivity, while the OC shell also offers robust protection. This work provides a simple and environmentally friendly strategy for developing practical heterogeneous catalysts that bring the synergistic effect into play between dilute alloy and functional carbon wrapping.

Lattice anchoring, in its varied forms, has proven effective at regulating the energetics of metastable phases of polymorphic crystals. Here, the authors utilize top-down photolithography to embed a tessellating 3D interfacial network into otherwise-unstable CsPbI3 perovskite thin films and devices, stabilizing the perovskite phase.

Abstract Objectives To analyze the changes of gene expression at different timepoints after spinal cord injury (SCI) with tenth segment thoracic injury. Methods Two SCI models, the complete paraplegia (H) and Allen’s strike (D) methods were applied to induce SCI in rats, and transcriptome sequencing was performed 1, 3, 7, 14, 56, and 70 days after SCI, respectively. Principal component analysis, differentially expressed gene analysis, and hierarchical clustering analysis were applied to analyze the differentially expressed genes (DEGs). Gene Ontology GO enrichment analysis, Kyoto Encyclopedia of Genes and Genomes enrichment analysis, and Gene Set Enrichment Analysis revealed the pathway of gene enrichment. Results There were 1,907, 3,120, 3,728, 978, 2,319, and 3,798 DEGs in the complete paraplegia group and 2,380, 878, 1,543, 6,040, 1,945, and 3,850 DEGs in the Allen’s strike method group and after SCI at 1, 3, 7, 14, 56, and 70 days, respectively. The transcriptome contours of D1, H1, D3, and H14 were clustered with C; the H56, D56, H70, and D70 transcriptome contours were similar and clustered together. H3, D7, and H7 were clustered together, and D14 was clustered separately. The transcriptome differences of the two SCI models were mainly concentrated during the first 2 weeks after SCI. The DEGs after SCI in the complete paraplegia group were more concentrated. Most of the early transcriptional regulation stabilized within 2 weeks after injury. Conclusions There were DEGs between the two SCI models. Through the gene changes and pathway enrichment of the entire time period after SCI, the molecular mechanism of SCI repair was revealed in depth, which provided a reference for SCI treatment in the future.

The fabrication of highly qualified patterns for photomasks using an electron beam writer is one of the key methods to achieve the requirements for high-end semiconductor devices manufacturing. We demonstrate in-situ examination of morphology changes in the electron-beam resist using super-resolution single-molecule fluorescence microscopy. An integrated scanning electron and fluorescence microscope was used to apply different e-beam doses on a PMMA model resist doped with fluorophores. The experiment shows that the density of fluorophores decreases at accumulated e-beam doses, revealed by the increasing average distances between the neighboring fluorophores before and after the e-beam treatment. The observation coincides with the film thinning in the e-beam treated sample area under AFM. Combining the data from super-resolution microscopy and AFM, we could identify different e-beam impact that is associated with e-beam doses. Particularly, we could observe certain degree of resist degradation outside the exposed area when high doses are applied. The degradation might be correlated to effects such as resist outgassing. This proof-of-concept illustrates a faster alternative method to AFM for characterizing the resist's morphological changes during e-beam processes.

Abstract Background Magnetic resonance imaging (MRI) is widely used for the evaluation of knee injuries, however, the accuracy of MRI in classifying multiple ligament knee injuries (MLKIs) remains unknown. This study aimed to investigate the accuracy of MRI in diagnosing and classifying acute traumatic MLKIs, we hypothesize that MRI had high accuracy in detecting and classifying MLKIs. Methods The clinical data of 97 patients who were diagnosed with acute traumatic MLKIs and managed by multi-ligament reconstruction between 2012 and 2020 were retrospectively reviewed. The MR images were read by two experienced radiologists and results were compared with intraoperative findings, which were considered as the reference for the identification of injured structures. The value of MRI in detecting injuries of anterior cruciate ligament (ACL), posterior cruciate ligament (PCL), medial collateral ligament (MCL), lateral collateral ligament (LCL), and meniscus was evaluated by calculating the sensitivity, specificity, positive predictive value, negative predictive value, positive likelihood ratio, negative likelihood ratio, and kappa coefficients analysis. The value of MRI in classifying MLKIs was evaluated by calculating the agreement between MRI and intraoperative findings. Results For detecting the specific injured structures in MLKIs, MRI had high sensitivity (90.7% for ACL, 90.4% for PCL, and moderate specificity (63.6% for ACL, 50% for PCL) in detecting cruciate ligament injuries, moderate sensitivity (79.1% for MCL, 55.6% for LCL) and specificity (46.7% for MCL, 68.4% for LCL) in detecting collateral ligament injuries, fair sensitivity (61.5%) and low specificity (39.4%) in the diagnosis of injuries to the meniscus. For classifying the MIKIs, MRI had a moderate agreement with intraoperative findings in classifying KD-V (kappa value = 0.57), poor agreement in the KD-I (kappa value = 0.39) and KD-IIIM (kappa value = 0.31), meaningless in the KD-II and KD-IIIL (kappa value

Reduced-dimensional halide perovskites are promising for light-emitting diodes but suffer from photo-degradation. Here Quan et al. identify the edge of the perovskite nanoplatelets as the degradation channels and use phosphine oxides to passivate the edges and boost device performance and lifetime.

Background. This study is aimed at to establish an effective prognostic nomogram for patients with atrial fibrillation (AF) and acute coronary syndrome (ACS) underwent percutaneous coronary intervention (PCI). Methods. The nomogram was based on a retrospective study of 977 patients with AF and ACS who underwent PCI who were admitted to any of the 11 tertiary hospitals in the Beijing area between 2009 and 2015. The predictive accuracy and discriminative ability of the nomogram were determined by a concordance index (C-index) and calibration curve and were compared using current risk scores such as GRACE, CRUSADE, CHA2DS2-VASc, and HAS-BLED. The results were validated using bootstrap resampling and a retrospective cohort study of 409 patients enrolled in Fuwai Hospital at the same institution. Results. Independent factors derived from multivariable analysis of the primary cohort to predict all-cause mortality were age, pattern of ACS, red blood cell distribution width, N-terminal proBNP, and serum creatinine, all of which were assembled into the nomogram. The calibration curve for the probability of recurrence showed that the nomogram-based predictions were in good agreement with actual observations. The C-index of the nomogram for predicting mortality was 0.764 (95% CI, 0.718-0.810), which was statistically higher than the C-index values for the current risk scores (from 0.573 to 0.681). In the validation cohort, the C-index of the nomogram for predicting all-cause death was 0.706 (95% CI 0.601-0.811), with no significant differences compared with GRACE and CRUSADE, but better than that of CHA2DS2-VASc and HAS-BLED. Conclusions. The nomogram has good prognostic prediction for patients with AF and ACS who underwent PCI.

A commercial interest in the improvement in the separation performance and permeability of porous materials is driving efforts to deeply explore new preparation methods. In this study, the porous silicate cement membranes (PSCMs) were successfully prepared through an adjustable combination of hot–dry casting and a cement hydration process. The obtained membrane channel was unidirectional, and the surface layer was dense. The physical characteristics of the PSCMs including their pore morphology, porosity, and compressive strength, were diversified by adjusting the solid content and hot–dry temperature. The results indicated that with the solid content increasing from 40 wt. % to 60 wt. %, the porosity decreased by 8.07%, while the compressive strength improved by 12.46%. As the hot–dry temperature increased from 40 °C to 100 °C, the porosity improved by 23.04% and the BET specific surface area and total pore volume enlarged significantly, while the compressive strength decreased by 27.03%. The pore size distribution of the PSCMs exhibited a layered structure of macropores and mesopores, and the pore size increased with the hot–dry temperature. Overall, the PSCMs, which had typical structures and adjustable physical characteristics, exhibited excellent permeability and separation performance.

Purpose: The purpose of this meta-analysis was to summarize the evidence of associations between obesity factors and spinal epidural lipomatosis (SEL) and to evaluate the strength and validity of these associations. Methods: Electronic databases such as Wiley Online Library, PubMed, Embase, Cochrane Library were searched and manual retrieval of references, the time limit was from the establishment of the database to May 2020. Methodological quality evaluations of the included studies were assessed using the bias risk assessment tool recommended by the Cochrane Guidelines. The RevMan 5.3 software was used for meta-analysis. Results: Finally, seven studies were included for meta-analysis, all of which were observational studies with mixed bias risk. These studies involved 807 patients, with an average age of 64 to 73.6 years, and 59.4 percent of the participants were male. The sample sizes for the included studies ranged from 28 to 288. The results of meta-analysis showed that high body mass index (BMI) was one of the factors affecting SEL ( P < 0.01, MD 1.37, 95% CI [0.81, 1.92]). All reviews had a high risk of bias, and the most common source of bias was that there was no strict unified case diagnosis standard between researches, and some studies (four items) did not clearly describe the confounders that they controlled. Conclusions: We suggest that physicians should consider high BMI as a factor leading to SEL, and to control body weight actively should be considered as the preferred treatment strategy before surgical intervention is conducted.

Using the cloud-ground lightning locating data from 2007 to 2017 and the thunderstorm day data from 1961 to 2003 in Hubei, we selected six lightning parameters such as thunderstorm day, cloud-ground lightning density, lightning current wave-head gradients, lightning current amplitude, large current density and small current density as risk evaluation indices of lightning disaster in Hubei province, and then combined them with the projection pursuit method to establish a lightning disaster risk assessment model. Based on this model, we conducted a risk zoning of lightning disasters in Hubei province. The results indicate that the lightning hazards in Hubei province can be divided into five risk levels, i.e., higher risk, sub high risk, medium risk, inferior risk and lower risk. The lightning disaster risk is higher in the east than that in the west, which gradually decreases from east to west. The higher and sub high risk areas mainly locate in the eastern regions of Hongan, Xiaochang, Xiaonan, Dongxihu, Caidian, Hannan and Honghu, while the inferior and lower risk areas mainly distribute in the western regions of Xiangzhou, Xiangcheng, Gucheng, Baokang, Xingshan, Zigui, Badong and Wufeng. The zone between the eastern and western regions mentioned above is mainly a medium-risk zone with a total of 27 districts or counties or cities. The results of lightning disaster risk zoning can provide scientific basis for local lightning disaster prevention and mitigation work for effective reduction of disaster losses.

The weak effects induced by lattice disorder on the optoelectronic properties of halide perovskites still remain elusive. Here Wu et al. confirm the indirect transition tail states in perovskite crystals which explain their low photoluminescence quantum yield, dual emission peaks and difficulties in realizing lasing.

Purpose: The purpose of this study is to explore if the surgical treatment will accelerate the progression of spinal cord injury (SCI) in patients with cervical Ossification of the posterior longitudinal ligament (OPLL) and if surgery will have better curative effect than conservative treatment. Methods: An extensive search of literature was implemented in PubMed, EMBASE, and other online databases. The quality of the included articles was evaluated according to the Newcastle-Ottawa Quality Assessment Scale, as recommended by the Cochrane manual, and meta-analysis was performed using the review manage5.3 software. Results: No obvious statistical difference was observed in the rate of SCI progression (P > 0.05, OR 1.15 [0.66, 2.00]), cervical range of motion, (P > 0.05, weighted mean difference (WMD) 4.52 [−5.75, 14.79]), and Japanese Orthopedic Association scores before surgery (P > 0.05, WMD −2.78 [−7.87, 2.32]) between the surgical group and conservative treatment group. However the surgical group illustrated obviously higher neurofunctional recovery rate (P < 0.05, OR 6.07 [1.55, 23.78]) and postoperative JOA score of the surgery group (P < 0.05, WMD −0.77 [−1.21, −0.33]) than conservative group. Conclusions: Based on this meta-analysis, there is not enough evidence to indicate that surgery will accelerate the progress of SCI with OPLL. However, the superiority of surgical efficacy can be observed over conservative treatment in terms of relieving neurological symptoms.

In order to study the distributions of the biomarker of the continental source rocks in the Sichuan Basin, 71 source rock samples were collected from the Upper Triassic-Lower Jurassic strata in different regions. The n- alkanes, isoprenoids, terpane, sterane, sesquiterpenes, caranes and aromatics in the extracts were analyzed in detail. GC-MS analysis has been conducted to analyze the biomarker of the continental source rocks. The results of GC-MS analysis indicate that the Upper Triassic source rocks are high in the content of extended tricyclic terpanes, pristane, phytane, gammacerane, C 28 regular sterane and carotene. However, they are low in content of rearranged compounds. The ratio of Pr/Ph is less than 1, with the characteristics of tricyclic terpane C 21 > C 23 . The Lower Jurassic source rocks are extremely low in content (even zero) of extended tricyclic terpanes, pristane, phytane, gammacerane, C 28 regular sterane and carotene, and high in content of rearranged compounds. The ratio of Pr/Ph is more than 1, with tricyclic terpane C 21 > C 23 . These characteristics are still preserved after maturation. Moreover, during the sedimentation of the source rocks of T 3 x 2 –T 3 x 3 members, the supply of continental plants was low (TAR C 29 , 1-MP/9-MP C 29 , 1-MP/9-MP

The performance of wide-bandgap perovskite photovoltaics is limited by the undesired phase transition and high density of deep level traps. Here, Tan et al. incorporate dipolar methylammonium cation to make the material defect-tolerant and achieve a high power conversion efficiency of 20.7%.

Abstract: The multi-stage minerals filled in pore space were sequenced, and the charging stages of fluid and hydrocarbon were reconstructed based on the observation of drilling cores and thin sections, homogeneous temperature testing of fluid inclusions, Laser Raman composition analysis and isotope geochemical analysis. The Cambrian Longwangmiao Formation in the study area went through 5 stages of fluid charging, in which 3 stages, mid-late Triassic, early-mid Jurassic and early-mid Cretaceous, were related to oil and gas charging. Especially the oil and gas charging event in early-mid Cretaceous was the critical period of gas accumulation in the study area, and was recorded by methane gas inclusions in the late stage quartz fillings. The 40Ar-39Ar dating of the 3rd stage methane inclusions shows that the natural gas charging of this stage was from 125.8±8.2 Ma. Analysis of Si, O isotopes and 87Sr/86Sr of the late stage quartz indicates that the fluid source of the quartz was formation water coming from long term evolution and concentration of meteoric water, but not from deep part or other sources, this also reflects that, in the critical charging period of natural gas, the Cambrian Longwangmiao Formation in Moxi structure had favorable conservation conditions for hydrocarbon accumulation, which was favorable for the formation of the Longwangmiao large natural gas pool. Key words: Sichuan Basin, Moxi structure, Cambrian Longwangmiao Formation, fluid charging, fluid inclusions, hydrocarbon accumulation

Formamidinium-lead-iodide-based perovskites have a preferred bandgap below 1.55 eV for solar cell applications but suffer from operational instability. Here, Zhao et al. improve the film quality using cesium-containing seeded growth to show high stabilized efficiency and more than 100 h lifetime under simulated sunlight.

The Bohai Bay region is a primary accumulation area of oil and gas in offshore China, in which overpressure commonly occurs in the Paleogene strata; the analysis on distribution characteristics and genetic mechanisms of the overpressure would provide geologic evidences for making plans of well drilling and logging as well as oil and gas exploitation; additionally, it could lay the geological foundation for studying how overpressure controlled hydrocarbon accumulation. Based on research, the overpressure of the study area starts from the second member of the Dongying Formation and ends in the third member of the Shahejie Formation. The distribution of overpressure is mainly controlled by the sag–salient tectonic framework within the basin, which means overpressure mainly develops in sags or slopes; however, high areas stay normal pressured. In the study area, pressure develops around Bozhong Sag and in northern Liaodong Bay reaches the peak. The genetic mechanisms of overpressures in the Paleogene reservoirs are mainly disequilibrium compaction, hydrocarbon generation of the organic matter, fluid charging, and transmission or the superimposition of the former two. Different strata have different genetic mechanisms of overpressure. The chief genetic mechanisms for the generation of overpressure of the Dongying Formation are disequilibrium compaction while the genesis of the formation of overpressure in the Shahejie Formation is more complicated in some extent. The first member of the Shahejie Formation dominated by disequilibrium compaction and hydrocarbon generation of the organic matter plays a supplemental role, while the second member of the Shahejie Formation, as the primary reservoir strata, is dominated by fluid charging and transmission, and the third member of the Shahejie Formation is the main source rock interval; its overpressure is closely related to hydrocarbon generation. Each contribution ratio for overpressure forming by different genetic mechanisms has been judged and figured out quantitatively according to geological, geophysical, and geochemical characteristics of the target strata.

Object: To investigate the efficacy of debridement, open drainage, and early feeding to treat early esophageal fistula complicated with anterior cervical surgery. Methods: Retrospective analysis was conducted on data from 3154 patients who had undergone anterior cervical surgeries for cervical vertebra diseases between January 2006 and January 2013, in which eight patients had esophageal fistula with five males and three females. Four patients had cervical spinal injuries and four patients had cervical spondylosis. All of whom had postoperative esophageal fistulas and underwent debridement and drainage. The wounds were left open for natural drainage. No drainage devices were placed in surgical sites, and no gastric tubes were placed after surgeries. Such normal food as rice balls but not liquid or semiliquid diet was recommended. Local pressurization was conducted with fists during feeding to prevent food overflowing from orificium fistulae. Results: Healed esophageal fistulas were achieved in all of the patients after 1–2 weeks treatment. There weren’t recurrent esophageal fistulas and delayed infection found during 2–5 years follow-up. Good swallowing functions and stable cervical vertebrae were achieved in all of the patients. The satisfactory therapeutic effects were achieved in patients with previous neck diseases. Frankel classifications were increased by 1–2 grades in patients with cervical spinal injuries. JOA scores were increased from 9.5 before surgery to 15.5 after surgery in patients with cervical vertebra diseases. Conclusions: Early postoperative esophageal fistula complication after anterior cervical surgery can successfully be treated by debridement, drainage without gastric tube, and with early oral postoperative feeding but without fluids.

The original version of this article incorrectly listed the present address of Bo Wu as ‘Present address: Institute of Electronic Paper Displays, South China Academy of Advanced Optoelectronics, South China Normal University, Guangzhou, Guangdong Province 510006, China’. This is the author’s primary affiliation. This has been corrected in both the PDF and HTML versions of the article.