149 results on '"Hai-Xia Wu"'
Search Results
2. Uncertainty aversion and farmers’ innovative seed adoption: Evidence from a field experiment in rural China
- Author
-
Hai-xia WU, Yan SONG, Le-shan YU, and Yan GE
- Subjects
ambiguity aversion ,risk aversion ,technology adoption ,field experiment ,Agriculture (General) ,S1-972 - Abstract
Based on the microdata of 705 wheat farmers in the Loess Plateau, this study empirically analyzes the impact of uncertainty on farmers’ adoption of innovative seeds using a field experiment. The results indicate that farmers are generally ambiguity-averse and risk-averse. In addition, farmers with higher ambiguity aversion and risk aversion are less likely to adopt innovative wheat seeds, where their risk aversion plays a dominant role. Enhancing information access will alleviate the negative influence of ambiguity aversion on farmers’ adoption of innovative seeds, and interlinked insurance and credit contracts will be beneficial to ease the adverse effect of risk aversion on the adoption of innovative wheat seeds. Meanwhile, heterogeneity analysis reveals that the inhibitory effects of ambiguity aversion and risk aversion on innovative seed adoption are more significant among farmers with lower education and household income. The government can establish both ex-ante and ex-post relevant guarantee mechanisms to help farmers preferably cope with various uncertainties in the production process, remitting farmers’ ambiguity aversion and risk aversion to enhance new agricultural technology adoption rates.
- Published
- 2023
- Full Text
- View/download PDF
3. Gut microbiota differs between two cold-climate lizards distributed in thermally different regions
- Author
-
Jun-Qiong Chen, Lu-Wen Zhang, Ru-Meng Zhao, Hai-Xia Wu, Long-Hui Lin, Peng Li, Hong Li, Yan-Fu Qu, and Xiang Ji
- Subjects
16S rRNA gene sequencing ,Cold-climate adaptation ,Gut microbiota ,Phrynocephalus erythrurus ,Phrynocephalus przewalskii ,Toad-headed lizard ,Ecology ,QH540-549.5 ,Evolution ,QH359-425 - Abstract
Abstract Background The metabolic cold-climate adaption hypothesis predicts that animals from cold environments have relatively high metabolic rates compared with their warm-climate counterparts. However, studies testing this hypothesis are sparse. Here, we compared gut microbes between two cold-climate lizard species of the genus Phrynocephalus to see if gut microbiota could help lizards adapt to cold environments by promoting metabolism. We conducted a 2 species (P. erythrurus and P. przewalskii) × 2 temperatures (24 and 30 °C) factorial design experiment, whereby we kept lizards of two Phrynocephalus species at 24 and 30 °C for 25 d and then collected their fecal samples to analyze and compare the microbiota based on 16S rRNA gene sequencing technology. Results The gut microbiota was mainly composed of bacteria of the phyla Proteobacteria, Firmicutes, Bacteroidetes, and Verrucomicrobia in both species (Proteobacteria > Firmicutes > Verrucomicrobiota in P. erythrurus, and Bacteroidetes > Proteobacteria > Firmicutes in P. przewalskii). Further analysis revealed that the gut microbiota promoted thermal adaptation in both lizard species, but with differences in the relative abundance of the contributory bacteria between the two species. An analysis based on the Kyoto Encyclopedia of Genes and Genomes revealed that the gut microbiota played important roles in metabolism, genetic information processing, cellular processes, and environmental information processing in both species. Furthermore, genes related to metabolism were more abundant in P. erythrurus at 24 °C than in other species ⋅ temperature combinations. Conclusion Our study provides evidence that gut microbiota promotes thermal adaptation in both species but more evidently in P. erythrurus using colder habitats than P. przewalskii all year round, thus confirming the role of gut microbiota in cold-climate adaptation in lizards.
- Published
- 2022
- Full Text
- View/download PDF
4. Revitalizing quality of life: a case report on the beneficial impact of comprehensive rehabilitation therapy in treating upper-limb lymphedema following breast cancer surgery
- Author
-
Yan-Fang Sui, Lang-Qian Tong, Xia-Fei Lin, Hai-Xia Wu, Jing-Qin Shi, Shan-Shan Wang, Bu-He Tang, and Zhen-Hua Song
- Subjects
breast cancer ,comprehensive rehabilitation ,functional brace ,lymphedema ,seven-step decongestion therapy ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
ObjectiveTo underscore the paramount significance of incorporating comprehensive rehabilitation therapy as a crucial aspect of managing lymphedema caused by breast cancer surgery, and to illuminate our first-hand experience and insights gained in utilizing this approach.MethodsWe present a case report of a breast cancer survivor who had been suffering from persistent left upper-limb edema for over 15 years, who was effectively treated with a combination of conventional rehabilitation (seven-step decongestion therapy) and a comprehensive rehabilitation program (seven-step decongestion therapy, along with core and respiratory function training, as well as functional brace wearing). The efficacy of the rehabilitation therapy was evaluated through a comprehensive assessmentResultsAlthough the patient underwent the conventional rehabilitation program for one month, only limited improvement was observed. However, after an additional month of comprehensive rehabilitation treatment, the patient exhibited significant improvement in both lymphedema and the overall function of the left upper limb. The patient’s progress was quantified by measuring the reduction in arm circumference, which demonstrated a notable decrease. Furthermore, improvements in joint range of motion were observed, with forward flexion of the shoulder enhancing by 10°, forward flexion improving by 15°, and elbow flexion increasing by 10°. In addition, manual muscular strength tests revealed an increase in strength from Grade 4 to Grade 5. The patient’s quality of life was also significantly improved, as evidenced by the increase in the Activities of Daily Living score from 95 to 100 points, the increase in the the Functional Assessment of Cancer Therapy: Breast score from 53 to 79 points, and the decrease in the Kessler Psychological Distress Scale score from 24 to 17 points.ConclusionWhile seven-step decongestion therapy has been shown to be effective in reducing upper-limb lymphedema caused by breast cancer surgery, it has limitations in treating more chronic cases of the condition. However, when combined with core and respiratory function training and functional brace wearing, seven-step decongestion therapy has been shown to be even more effective in reducing lymphedema and improving limb function, ultimately leading to significant improvements in quality of life.
- Published
- 2023
- Full Text
- View/download PDF
5. Which evaluation criteria of the short-term efficacy can better reflect the long-term outcomes for patients with nasopharyngeal carcinoma?
- Author
-
Li-qin Ma, Hai-xia Wu, Xiang-quan Kong, Zhao-dong Fei, Wei-ning Fang, Kai-xin Du, Fei Chen, Dan Zhao, and Zhu-peng Wu
- Subjects
Nasopharyngeal carcinoma ,Induction chemotherapy ,Tumor measurement ,RECIST ,Response assessment ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Purpose: To compare the consistency of one-dimensional Response Evaluation Criteria in Solid Tumors (1D-RECIST), two-dimensional WHO criteria (2D-WHO), and three-dimensional (3D) measurement for therapeutic response assessment of nasopharyngeal carcinoma (NPC). Materials and methods: Retrospective data of 288 newly diagnosed NPC patients were reviewed. Tumor size was assessed on magnetic resonance imaging (MRI) according to the 1D-RECIST, 2D-WHO, and 3D measurement criteria. Agreement between tumor responses was assessed using unweighted k statistics. The receiver operating characteristic (ROC) curve was used to determine the optimal cut-off point of the PTV. The Kaplan–Meier method and Cox regression were used for the survival analysis. Results: The optimal cut-off point of the PTV for progression-free survival (PFS) was 29.6%. Agreement with PTV measurement was better for 1D measurement than for 2D and 3D measurements (kappa values of 0.646, 0.537, and 0.577 for 1D, 2D, and 3D measurements, respectively; P
- Published
- 2022
- Full Text
- View/download PDF
6. The insights into the evolutionary history of Translucidithyrium: based on a newly-discovered species
- Author
-
Xinhao Li, Hai-Xia Wu, Jinchen Li, Hang Chen, and Wei Wang
- Subjects
Botany ,QK1-989 - Abstract
During the field studies, a Translucidithyrium-like taxon was collected in Xishuangbanna of Yunnan Province, during an investigation into the diversity of microfungi in the southwest of China. Morphological observations and phylogenetic analysis of combined LSU and ITS sequences revealed that the new taxon is a member of the genus Translucidithyrium and it is distinct from other species. Therefore, Translucidithyrium chinense sp. nov. is introduced here. The Maximum Clade Credibility (MCC) tree from LSU rDNA of Translucidithyrium and related species indicated the divergence time of existing and new species of Translucidithyrium was crown age at 16 (4–33) Mya. Combining the estimated divergence time, paleoecology and plate tectonic movements with the corresponding geological time scale, we proposed a hypothesis that the speciation (estimated divergence time) of T. chinense was earlier than T. thailandicum. Our findings provided new insights into the species of Translucidithyrium about ecological adaptation and speciation in two separate areas.
- Published
- 2020
- Full Text
- View/download PDF
7. Taxonomy, Phylogenetic and Ancestral Area Reconstruction in Phyllachora, with Four Novel Species from Northwestern China
- Author
-
Jin-Chen Li, Hai-Xia Wu, Yuying Li, Xin-Hao Li, Jia-Yu Song, Nakarin Suwannarach, and Nalin N. Wijayawardene
- Subjects
Ancestral reconstruction ,Multigene phylogeny ,Morphology ,four new taxa ,Phyllachora ,Biology (General) ,QH301-705.5 - Abstract
The members of Phyllachora are biotrophic, obligate plant parasitic fungi featuring a high degree of host specificity. This genus also features a high degree of species richness and worldwide distribution. In this study, four species occurring on leaf and stem of two different species of grass were collected from Shanxi and Shaanxi Provinces, China. Based on morphological analysis, multigene (combined data set of LSU, SSU, and ITS) phylogenetic analyses (maximum likelihood and Bayesian analysis), and host relationship, we introduce herein four new taxa of Phyllachora. Ancestral area reconstruction analysis showed that the ancestral area of Phyllachora occurred in Latin America about 194 Mya. Novel taxa are compared with the related Phyllachora species. Detailed descriptions, illustrations, and notes are provided for each species.
- Published
- 2022
- Full Text
- View/download PDF
8. Retrospective study of topical medical treatment for glaucoma
- Author
-
Xiu-Juan Guo, Di Chen, Hai-Xia Wu, Yuan-Bo Liang, and Shu-Ke Luo
- Subjects
glaucoma ,topical medication ,changing patterns ,Ophthalmology ,RE1-994 - Abstract
AIM:To document the change in pattern of topical anti-glaucoma medicine by retrospective analysis in the outpatient of a local tertiary eye hospital(Second People's Hospital of Foshan)in China during 2009-2016. METHODS: The data of topical anti-glaucoma medications in outpatients in our hospital were collected from January 1st 2009 to December 31st 2016. These data were analyzed retrospectively. The main outcome measures were the percentages of prescriptions for all kinds of anti-glaucoma medications, the ratio of the mono-therapy and combination therapies, and the defined daily doses. RESULTS: The ratio of beta-adrenergic receptor blocker declined from 81.51% in 2009 to 69.37% in 2016. The use of cholinergic agent decreased from 12.04% in 2009 to 3.78% in 2016. The prostaglandin derivatives increased from 8.40% in 2009 to 31.40% in 2016. Alpha 2 adrenergic receptor agonists increased from 0 in 2009 to 29.44% in 2016. Local carbonic anhydrase inhibitors changed slightly. The proportion of mono-therapy was reduced from 65.83% in 2009 to 46.22% in 2016. The ration of two agents combination increased from 32.49% in 2009 to 38.77% in 2016. The three medications combination was increased from 1.68% in 2009 to 12.96% in 2016. The proportion of four medications combination increased from 0 in 2009 to 1.97% in 2016, while the combined use of five drugs was an isolated case. CONCLUSION: The proportion of topical anti-glaucoma agents has changed remarkably. The beta-adrenergic receptor blockers and cholinergic medications are decreasing, while the prostaglandin derivatives and alpha 2 adrenergic receptor agonists are increasing. The proportion of mono-therapy is declining, while the combined therapies are rising.
- Published
- 2018
- Full Text
- View/download PDF
9. Structure, Distribution, Chemical Composition, and Gene Expression Pattern of Glandular Trichomes on the Leaves of Rhus potaninii Maxim
- Author
-
Qin Lu, Nawaz Haider Bashir, Hai-Xia Wu, Weiwei Wang, Jinwen Zhang, Yongzhong Cui, and Hang Chen
- Subjects
distribution pattern ,gene expression pattern ,glandular trichome ,histological structure ,secretion ,plant defense ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Rhus potaninii Maxim is an economically and medicinally important tree species in China. It produces galls (induced by aphids) with a high abundance of tannins. Here, we discuss the histology, cellular structures and their distribution, and the macromolecular components of secretive glandular trichomes on the leaves of R. potaninii. A variation in the density of glandular trichomes and tomenta was found between the adaxial and abaxial sides of a leaf in different regions and stages of the leaf. The glandular trichomes on R. potaninii trees comprise a stalk with no cellular structure and a head with 8–15 cells. Based on staining, we found that the secretion of glandular trichomes has many polysaccharides, phenolic compounds, and acidic lipids but very few neutral lipids. The dense glandular trichomes provide mechanical protection for young tissues; additionally, their secretion protects the young tissues from pathogens by a special chemical component. According to transcriptome analysis, we found enhanced biosynthetic and metabolism pathways of glycan, lipids, toxic amino acids, and phenylpropanoids. This shows a similar tendency to the staining. The numbers of differentially expressed genes were large or small; the averaged range of upregulated genes was greater than that of the downregulated genes in most subpathways. Some selectively expressed genes were found in glandular trichomes, responsible for the chitinase activity and pathogenesis-related proteins, which all have antibacterial activity and serve for plant defense. To our knowledge, this is the first study showing the components of the secretion from glandular trichomes on the leaf surface of R. potaninii.
- Published
- 2021
- Full Text
- View/download PDF
10. Variation and diversification of the microbiome of Schlechtendalia chinensis on two alternate host plants.
- Author
-
Hai-Xia Wu, Xiaoming Chen, Hang Chen, Qin Lu, Zixiang Yang, Weibin Ren, Juan Liu, Shuxia Shao, Chao Wang, Kirst King-Jones, and Ming-Shun Chen
- Subjects
Medicine ,Science - Abstract
Schlechtendalia chinensis, a gall-inducing aphid, has two host plants in its life cycle. Its wintering host is a moss (typically Plagiomnium maximoviczii) and its main host is Rhus chinensis (Sumac), on which it forms galls during the summer. This study investigated bacteria associated with S. chinensis living on the two different host plants by sequencing 16S rRNAs. A total of 183 Operational Taxonomic Units (OTUs) from 50 genera were identified from aphids living on moss, whereas 182 OTUs from 49 genera were found from aphids living in Sumac galls. The most abundant bacterial genus among identified OTUs from aphids feeding on both hosts was Buchnera. Despite similar numbers of OTUs, the composition of bacterial taxa showed significant differences between aphids living on moss and those living on R. chinensis. Specifically, there were 12 OTUs from 5 genera (family) unique to aphids living on moss, and 11 OTUs from 4 genera (family) unique to aphids feeding in galls on R. chinensis. Principal Coordinate Analysis (PCoA) also revealed that bacteria from moss-residing aphids clustered differently from aphids collected from galls. Our results provide a foundation for future analyses on the roles of symbiotic bacteria in plant-aphid interactions in general, and how gall-specific symbionts differ in this respect.
- Published
- 2018
- Full Text
- View/download PDF
11. Crystal structure of diaquabis(bicyclo[2.2.1]hept-5-ene-2,3-dicarboxylate-κ4O, O′:/O′′,O′′′)bis-(2,2′-bipyridine-κ2N, N′)dicadmium(II) hydrate
- Author
-
Hai-Xia Wu, Hai-Yan Zhang, and Ling-Yun Xin
- Subjects
1434839 ,Physics ,QC1-999 ,Crystallography ,QD901-999 - Abstract
The complex: C19H18CdN2O5, Triclinic, P-1, a = 9.6654(11) Å, b = 10.5458(12) Å, c = 10.7081(12) Å, α = 108.5220(10)°, β = 92.0640(10)°, γ = 111.4760(10)°, V = 948.62(19) Å3, Rgt(F) = 0.0200, wRref(F2) = 0.0535, Z = 2, T = 293(2) K.
- Published
- 2016
- Full Text
- View/download PDF
12. Crystal structure of catena-poly[aqua-(μ2-5-norbornene-2,3-dicarboxylato-κ3O,O′:O′′)-(1,10-phenanthroline-κ2N,N′)zinc(II)], [Zn(C9H8O4)(C12H8N2)(H2O)]n
- Author
-
Hai-Xia Wu, Ling-Yun Xin, Ying-Ge Zhang, and Yan-Yan Zhang
- Subjects
1267/4368 ,Physics ,QC1-999 ,Crystallography ,QD901-999 - Abstract
The complex : C21H18N2O5Zn, Monoclinic, P2(1)/n, a = 12.4476(15) Å, b = 9.1446(11) Å, c = 17.420(2) Å, β = 98.012(2), V = 1963.6(4) Å3, Rgt(F) = 0.0370, wRref(F2) = 0.0867; Z = 4; T = 296(2) K.
- Published
- 2015
- Full Text
- View/download PDF
13. Electrospun N-halamine/ZnO-based platform eradicates bacteria through multimodal antimicrobial mechanism of action
- Author
-
Hui-Hui Qu, Cong Wang, Yi-Xuan Guo, Zi-Ying Zhao, Long Qiao, Jie-Bing Yang, Hai-Xia Wu, Quan-Shun Li, and Alideertu Dong
- Subjects
Materials Chemistry ,Metals and Alloys ,Physical and Theoretical Chemistry ,Condensed Matter Physics - Published
- 2022
14. Taxonomy and evolution history of two new litter-decomposing Ciliochorella (Amphisphaeriales, Sporocadaceae).
- Author
-
Jia-Yu Song, Hai-Xia Wu, Jin-Chen Li, Wei-Feng Ding, Cui-Ling Gong, Xiang-Yu Zeng, Wijayawardene, Nalin N., and Da-Xin Yang
- Subjects
- *
FOREST litter , *SAVANNAS , *TAXONOMY , *LACCASE , *PALEOGENE , *CELLULASE - Abstract
The genus Ciliochorella is a group of pestalotioid fungi, which typically occurs in subtropical and tropical areas. Species from the Ciliochorella genus play important roles in the decomposition of litter. In this study, we introduce two new species (Ciliochorella chinensis sp. nov. and C. savannica sp. nov.) that were found on leaf litter collected from savanna-like vegetation in hot dry valleys of southwestern China. Phylogenetic analyses of combined LSU, ITS and tub2 sequence datasets indicated that C. chinensis and C. savannica respectively form a distinct clade within the Ciliochorella genus. The comparison of the morphological characteristics indicated that the two new species are well differentiated within this genus species. Analysis of the evolutionary history suggests that Ciliochorella originated from the Eurasian continent during the Paleogene (38 Mya). Further, we find that both new species can produce cellulase and laccase, playing a decomposer role. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
15. Metagenomic analysis suggests unique gut‐microbiota composition and GH family constitution of Macrotermes annandalei
- Author
-
Yongzhong Cui, Weiwei Wang, Qin Lu, Hai‐Xia Wu, Xiaofei Ling, Juan Liu, Jinwen Zhang, and Hang Chen
- Subjects
Insect Science - Published
- 2022
16. Yunnan–Guizhou Plateau: a mycological hotspot
- Author
-
Yanxia Liu, Lakmali S. Dissanayake, Hai-Xia Wu, Dong-Qi Dai, Yuan-Pin Xiao, Ting-Chi Wen, Saowaluck Tibpromma, Nalin N. Wijayawardene, Chun-Ying Deng, Ji-Chuan Kang, Yong Wang, Li-Zhou Tang, Samantha C. Karunarathna, Qi-Rui Li, Yingqian Kang, Xiang-Chun Shen, and Huang Zhang
- Subjects
geography ,Ophiocordycipitaceae ,Plateau ,geography.geographical_feature_category ,biology ,Ecology ,Hypocreales ,Tolypocladium ,Species diversity ,Plant Science ,Sordariomycetes ,biology.organism_classification ,Biodiversity hotspot ,Taxonomy (biology) ,Ecology, Evolution, Behavior and Systematics - Abstract
Guizhou and Yunnan Provinces (Yungui Plateau) in Southwestern China are well known as biodiversity hotspots. We introduce two new species in this study viz., Mucispora hydei (in Fuscosporellaceae, Fuscosporellales, Sordariomycetes) and Tolypocladium cucullae (Ophiocordycipitaceae, Hypocreales, Sordariomycetes) and six new records based on morpho-molecular analyses. Full descriptions, color photographs and phylogenetic trees to indicate the placements of new species are provided.
- Published
- 2021
17. Ag/AgX nanostructures serving as antibacterial agents: achievements and challenges
- Author
-
Alideertu Dong, Hai-Xia Wu, and Pei-Pei Li
- Subjects
Materials science ,Nanostructure ,Human life ,Visible light irradiation ,Metals and Alloys ,Light irradiation ,Nanotechnology ,Condensed Matter Physics ,Antimicrobial ,Nanomaterials ,Materials Chemistry ,Photocatalysis ,Physical and Theoretical Chemistry ,Antibacterial agent - Abstract
Bacterial infections, especially the frequently emerging “superbugs”, seriously affect the quality of human life and even threaten human health. As the emerging antimicrobial agents that effectively eradicate pathogens, nanomaterials have been widely explored due to their effectiveness against wide-spectrum bacteria and “superbugs”. Of them, Ag/AgX nanostructures (X representing Cl, Br or I) have emerged as an excellent antibacterial agent because of their excellent photocatalytic performance in inactivating pathogens under light irradiation, which provides a new opportunity for the development of high-efficient visible-light driven photocatalytic sterilization. To date, Ag/AgX nanostructures have been widely employed in antibacterial associated fields because they are efficient in producing reactive oxygen species (ROS) and reactive chlorine species (RCS) under visible light irradiation. In this review, we summarized the recent progress of Ag/AgX nanostructures as plasmonic photocatalysts in the antibacterial field, focusing on the antibacterial effects and mechanisms of Ag/AgX nanostructures, as well as their potent applications. Finally, the challenges and prospects of Ag/AgX nanostructures acting as active antibacterial agents were discussed.
- Published
- 2021
18. Obstetric and neonatal outcomes after frozen–thawed embryos transfer with laser-assisted hatching: a retrospective cohort study
- Author
-
Mei Zhao, Peng Cheng Kong, Hai Xia Wu, Jia Ping Pan, Shan Shan Liang, Mei Yuan Huang, Xiao Ming Teng, and Yi Ping Liu
- Subjects
China ,medicine.medical_specialty ,Placenta ,Fertilization in Vitro ,Miscarriage ,Preeclampsia ,Pregnancy ,medicine ,Fetal macrosomia ,Humans ,reproductive and urinary physiology ,Retrospective Studies ,Placental abruption ,business.industry ,Obstetrics ,Lasers ,Infant, Newborn ,Pregnancy Outcome ,Obstetrics and Gynecology ,General Medicine ,medicine.disease ,female genital diseases and pregnancy complications ,Embryo transfer ,Gestational diabetes ,embryonic structures ,Premature Birth ,Female ,business ,Premature rupture of membranes - Abstract
To ascertain if assisted hatching (AH) increases the risk of placenta-associated diseases and perinatal outcomes after frozen–thawed cleavage-stage embryo transfer. We retrospectively evaluated 924 women who conceived with frozen–thawed cleavage-stage embryos transfer with (n = 390) or without (n = 534) laser-AH between 2013 and 2015. Data were obtained from the database on in vitro fertilization (IVF) patients in Shanghai First Maternity and Infant Hospital. We assessed neonatal (preterm birth, low birthweight, fetal macrosomia, stillbirth) and obstetric (miscarriage, ectopic pregnancy, post-term pregnancy, gestational diabetes (GDM), preeclampsia, intrahepatic cholestasis (ICP), placenta previa, placental abruption, premature rupture of membranes) outcomes. In twins, the median birthweight was lower in the AH group than that in the control group, and the prevalence of low birthweight (
- Published
- 2021
19. A new species of foliicolous lichenized fungi from southwest China: Calopadia ruiliensis sp. nov
- Author
-
Xin-Hao Li, Wei-Bin Ren, Zi-Xiang Yang, and Hai-Xia Wu
- Subjects
Geography ,biology ,Botany ,Plant Science ,Foliicolous ,Calopadia ,China ,biology.organism_classification - Published
- 2021
20. Gut microbiome contributes to cold-climate adaptation in lizards
- Author
-
Jun-Qiong Chen, Lu-Wen Zhang, Ru-Meng Zhao, Hai-Xia Wu, Long-Hui Lin, Peng Li, Hong Li, Yan-Fu Qu, and Xiang Ji
- Abstract
The metabolic cold-climate adaption hypothesis predicts that animals from cold environments have relatively high metabolic rates compared with their warm-climate counterparts. However, studies testing this hypothesis are sparse. Here, we compared gut microbes between two cold-climate lizard species of the genus Phrynocephalus to test the hypothesis that gut microbiota can help lizards adapt to cold environments by promoting metabolism and absorption efficiency. We kept lizards at 24°C and 30°C for 25 d, and then collected their fecal samples to analyze and compare the microbiota based on 16S rRNA gene sequencing technology. The gut microbiota was mainly composed of Proteobacteria, Firmicutes, Bacteroidetes, and Verrucomicrobia at the phylum level in both species (Proteobacteria > Firmicutes > Verrucomicrobiota in P. erythrurus, and Bacteroidota > Proteobacteria > Firmicutes in P. przewalskii). Further analysis revealed that the gut microbiota contributed to the host’s cold adaptation, but with differences in the relative abundance of these contributory bacteria between the two species. KEGG analysis revealed that the gut microbiota primarily played roles in metabolism, genetic information processing, cellular processes, and environmental information processing in both species. Furthermore, genes related to metabolism were more abundant in P. erythrurus at 24 °C than in other species × temperature combinations, indicating the role of gut microbiota in long-term cold-climate adaptation. Our finding that gut microbiome contributes to cold-climate adaptation in both species but more evidently in P. erythrurus using colder habitats than P. przewalskii throughout a year confirms the gut microbiota’s role in the cold-climate adaptation in lizards.IMPORTANCEThis study proves that temperature affects the composition and relative abundance of the gut microbiota in two Phrynocephalus lizards in a species-specific manner. Both species harbor specific gut microbiota with significant roles in cold-climate adaptation. Specifically, P. erythrurus has a higher Proteobacteria ratio and relative abundance of metabolism-related microbial genes in the gut than P. przewalskii. Given that P. erythrurus uses colder habitats than P. przewalskii throughout a year, these results suggest that gut microbiota contributes to cold-climate adaptation in both species lizards but more evidently in P. erythrurus. This study provides evidence linking gut microbiome with cold adaptation. The co-evolution mechanism between gut microbiota and their hosts in extreme environments will provide new insights into animal adaptation.
- Published
- 2022
21. The insights into the evolutionary history of Translucidithyrium: based on a newly-discovered species
- Author
-
Wei Wang, Hang Chen, Hai-Xia Wu, Xinhao Li, and Jinchen Li
- Subjects
0106 biological sciences ,China ,Microfungi ,Biology ,phylogeny ,010603 evolutionary biology ,01 natural sciences ,030308 mycology & parasitology ,03 medical and health sciences ,taxonomy ,Ascomycota ,Phylogenetics ,Translucidithyrium ,lcsh:Botany ,Clade ,Divergence time ,Ecology, Evolution, Behavior and Systematics ,new species ,0303 health sciences ,Phaeothecoidiellaceae ,Phylogenetic tree ,Fungi ,Capnodiales ,lcsh:QK1-989 ,Taxon ,morphological characteristics ,speciation ,Evolutionary Ecology ,Evolutionary biology ,Dothideomycetes ,Paleoecology ,Taxonomy (biology) ,Research Article - Abstract
During the field studies, a Translucidithyrium-like taxon was collected in Xishuangbanna of Yunnan Province, during an investigation into the diversity of microfungi in the southwest of China. Morphological observations and phylogenetic analysis of combined LSU and ITS sequences revealed that the new taxon is a member of the genus Translucidithyrium and it is distinct from other species. Therefore, Translucidithyrium chinensesp. nov. is introduced here. The Maximum Clade Credibility (MCC) tree from LSU rDNA of Translucidithyrium and related species indicated the divergence time of existing and new species of Translucidithyrium was crown age at 16 (4–33) Mya. Combining the estimated divergence time, paleoecology and plate tectonic movements with the corresponding geological time scale, we proposed a hypothesis that the speciation (estimated divergence time) of T. chinense was earlier than T. thailandicum. Our findings provided new insights into the species of Translucidithyrium about ecological adaptation and speciation in two separate areas.
- Published
- 2020
22. Refined families of Dothideomycetes: orders and families incertae sedis in Dothideomycetes
- Author
-
Ausana Mapook, Digvijayini Bundhun, Kasun M. Thambugala, Jian-Kui Liu, Subashini C. Jayasiri, V. Venkateswara Sarma, Danushka S. Tennakoon, Ave Suija, Mekala Niranjan, Felix Schumm, Gareth E.B. Jones, Xiang-Yu Zeng, Gang Liu, Alan J. L. Phillips, Jadson D. P. Bezerra, Hiroyuki Kashiwadani, Nimali I. de Silva, Hong-Bo Jiang, Diana Sandamali, Rajesh Jeewon, Milan C. Samarakoon, Dhandevi Pem, Zong-Long Luo, Jing Yang, K. W. Thilini Chethana, Satinee Suetrong, Mingkwan Doilom, Ishara S. Manawasinghe, Jayarama D. Bhat, Huang Zhang, Sinang Hongsanan, Anuruddha Karunarathna, Nalin N. Wijayawardene, Hiran A. Ariyawansa, Ning Xie, Rungtiwa Phookamsak, Hai-Xia Wu, Yao Feng, Jutamart Monkai, Dulanjalee Harishchandra, Yong-Zhong Lu, Pranami D. Abeywickrama, Putarak Chomnunti, Dong-Qin Dai, Emmanuël Sérusiaux, Vinodhini Thiyagaraja, Ricardo Miranda-González, Chayanard Phukhamsakda, Saowaluck Tibpromma, André Aptroot, Chitrabhanu S. Bhunjun, Anusha H. Ekanayaka, Asha J. Dissanayake, Eric H. C. McKenzie, Napalai Chaiwan, Ruvishika S. Jayawardena, Shu-Hua Jiang, Jiesheng Zheng, Sheng-Nan Zhang, Chada Norphanphoun, Sergio Pérez-Ortega, Chanokned Senwanna, Qing Tian, Jin-Feng Zhang, Kevin D. Hyde, Jianchu Xu, Damien Ertz, Monika C. Dayarathne, Ning-Guo Liu, Cécile Gueidan, B. Devadatha, Dhanushka N. Wanasinghe, Dan-Feng Bao, Subodini N. Wijesinghe, Achala R. Rathnayaka, Robert Lücking, Saranyaphat Boonmee, and Kwang Hee Moon
- Subjects
Taxon ,Ecology ,Phylogenetic tree ,Pleosporomycetidae ,biology ,Phylogenetics ,Evolutionary biology ,Dothiorella ,Dothideomycetidae ,Dothideomycetes ,biology.organism_classification ,Incertae sedis ,Ecology, Evolution, Behavior and Systematics - Abstract
Numerous new taxa and classifications of Dothideomycetes have been published following the last monograph of families of Dothideomycetes in 2013. A recent publication by Honsanan et al. in 2020 expanded information of families in Dothideomycetidae and Pleosporomycetidae with modern classifications. In this paper, we provide a refined updated document on orders and familiesincertae sedisof Dothideomycetes. Each family is provided with an updated description, notes, including figures to represent the morphology, a list of accepted genera, and economic and ecological significances. We also provide phylogenetic trees for each order. In this study, 31 orders which consist 50 families are assigned as ordersincertae sedisin Dothideomycetes, and 41 families are treated as familiesincertae sedisdue to lack of molecular or morphological evidence. The new order, Catinellales, and four new families,Catinellaceae,Morenoinaceae NeobuelliellaceaeandThyrinulaceaeare introduced. Seven genera (Neobuelliella,Pseudomicrothyrium,Flagellostrigula,Swinscowia,Macroconstrictolumina,Pseudobogoriella, andSchummia) are introduced. Seven new species (Acrospermum urticae,Bogoriella complexoluminata,Dothiorella ostryae,Dyfrolomyces distoseptatus,Macroconstrictolumina megalateralis,Patellaria microspora, andPseudomicrothyrium thailandicum) are introduced base on morphology and phylogeny, together with two new records/reports and five new collections from different families. Ninety new combinations are also provided in this paper.
- Published
- 2020
23. Two new species of Phaeosaccardinula from Southwest China
- Author
-
Chao Deng, Chang-Hua Gu, Hai-Xia Wu, Jian-Min Wang, Hui Yang, and Zhi-Chang Zhang
- Subjects
Taxon ,biology ,Phylogenetic tree ,Genus ,Evolutionary biology ,Morphology (biology) ,Plant Science ,Internal transcribed spacer ,Phaeosaccardinula ,biology.organism_classification ,Ribosomal DNA ,Ecology, Evolution, Behavior and Systematics ,Molecular analysis - Abstract
Two new fungal species, Phaeosaccardinula dalbergiae and Phaeosaccardinula yunnanensis collected from Yunnan Province, China are described based on morphology and molecular data. They are compared with related taxa of Phaeosaccardinula. Detailed description, illustrations and notes are provided for each species. Molecular analysis based on the phylogenetic tree of combined internal transcribed spacer and large subunit nuclear ribosomal DNA sequences show that the two species form distinct clusters within the genus Phaeosaccardinula.
- Published
- 2020
24. Refined families of Dothideomycetes: Dothideomycetidae and Pleosporomycetidae
- Author
-
Janith V. S. Aluthmuhandiram, Dhanushka N. Wanasinghe, E. B. G. Jones, Yin Lu, Cécile Gueidan, Jadson D. P. Bezerra, R. Phookamsak, Dulanjalee Harishchandra, Jutamart Monkai, Subashini C. Jayasiri, Jian-Kui Liu, D. S. Sandamali, Milan C. Samarakoon, Saranyaphat Boonmee, Satinee Suetrong, Kasun M. Thambugala, Ishara S. Manawasinghe, N. Chaiwan, Sirinapa Konta, G. Liu, Kwang Hee Moon, K. W. T. Chethana, Achala R. Rathnayaka, V. Venkateswara Sarma, Robert Lücking, M. Niranjan, Dan-Feng Bao, Y. Feng, Emmanuël Sérusiaux, N. I. de Silva, Anuruddha Karunarathna, Kevin D. Hyde, Nalin N. Wijayawardene, Danushka S. Tennakoon, Hai-Xia Wu, Subodini N. Wijesinghe, Mingkwan Doilom, Haiyan Zhang, Darbhe J. Bhat, Hiroyuki Kashiwadani, Alan J. L. Phillips, Jing Yang, Chitrabhanu S. Bhunjun, Jianchu Xu, Junyan Zhang, H. B. Jiang, S. N. Zhang, Sergio Pérez-Ortega, Pranami D. Abeywickrama, Rajesh Jeewon, André Aptroot, Ave Suija, Ausana Mapook, Felix Schumm, Digvijayini Bundhun, Damien Ertz, Monika C. Dayarathne, Ning-Guo Liu, B. Devadatha, Xiang Yu Zeng, Chanokned Senwanna, Qing Tian, Putarak Chomnunti, S. Hongsanan, J. S. Zheng, Chayanard Phukhamsakda, Dhandevi Pem, Zong-Long Luo, Hiran A. Ariyawansa, Ning Xie, Anusha H. Ekanayaka, Ruvishika S. Jayawardena, Shu-Hua Jiang, Dong-Qin Dai, Chada Norphanphoun, Vinodhini Thiyagaraja, Ricardo Miranda-González, Saowaluck Tibpromma, Asha J. Dissanayake, and Eric H. C. McKenzie
- Subjects
Dothideales ,Hysteriales ,Myriangiales ,500 Naturwissenschaften und Mathematik::570 Biowissenschaften ,Biologie::579 Mikroorganismen, Pilze, Algen ,biology ,Dothideomycetidae ,Plant Science ,Dothideomycetes ,Capnodiales ,biology.organism_classification ,6 new taxa ,Pleosporomycetidae ,Evolutionary biology ,Mytilinidiales ,new family ,Pleosporales ,Taxonomy (biology) ,Gloniales ,Ecology, Evolution, Behavior and Systematics - Abstract
The class Dothideomycetes is the largest and most ecologically diverse class of fungi, comprising endophytes, epiphytes, saprobes, human and plant pathogens, lichens, and lichenicolous, nematode trapping and rock-inhabiting taxa. Members of this class are mainly characterized by bitunicate asci with fissitunicate dehiscence, and occur on broad range of hosts in aquatic and terrestrial habitats. Since the last monograph of families of Dothideomycetes in 2013, numerous novel species, genera, families and orders have been discovered. This has expanded information which has led to the modern classification in Dothideomycetes. In this paper, we provide a refined updated document on families of Dothideomycetes with emphasis on Dothideomycetidae and Pleosporomycetidae. We accept three orders with 25 families and four orders with 94 families in Dothideomycetidae and Pleosporomycetidae, respectively. The new family Paralophiostomataceae is introduced in Pleosporales. Each family is provided with an updated description, notes, including figures to represent the morphology, list of accepted genera, and economic and ecological significances. We also provide an overall phylogenetic tree of families in Dothideomycetes based on combined analysis of LSU, SSU, rpb-2 and tefl sequence data, and phylogenetic trees for each order in Dothideomycetidae and Pleosporomycetidae. Family-level trees are provided for the families which include several genera such as Mycosphaerellaceae and Teratosphaeriaceae. Two new genera (Ligninsphaeriopsis and Paralophiostoma) are introduced. Five new species (Biatrisopora borsei, Comoclathris galatellae, Ligninsphaeriopsis thailandica, Paralophiostoma hysterioides and Torula thailandica) are introduced based on morphology and phylogeny, together with nine new reports and seven new collections from different families.
- Published
- 2020
25. Tick (Acari: Ixodoidea) Fauna and Zoogeographic Division of Jiangxi Province, China
- Author
-
Jun-Hua Tian, Kun Li, Shao-Zai Zhang, Zhong-Ji Xu, Hai-Xia Wu, Hong-Bin Xu, and Chao-Liang Lei
- Subjects
Infectious Diseases ,Insect Science ,Parasitology ,Microbiology - Abstract
Tick fauna and zoogeographic distribution of Jiangxi Province remain largely unknown due to the lack of data on distribution, occurrence, and host associations of ticks. Considering this, we collected 1,817 individual samples from natural hosts, humans, and vegetation in 18 counties/districts throughout Jiangxi Province, China, from 2015 to 2021. These 1,817 individuals were found to 13 tick species, 4 genera, and 1 family. In addition, the tick sample data from 8 sampling localities (counties and districts) reported in previous studies were also included in our data. A total of 4,021 individuals, including our sample collection and the previously reported data, were assigned to at least 18 species, 6 genera, and 2 families. One newly recorded species Dermacentor sp. (near D. steini Schulze) was found; three misidentified species (Ixodes acuminatus, Haemaphysalis spinigera, and Haemaphysalis verticalis) reported previously were deleted; and one misidentified species Dermacentor auratus Supino was revised as Dermacentor steini Schulze. In addition, we divided the tick fauna in Jiangxi Province into 5 zoogeographic areas and assigned the 18 tick species collected from 26 localities to these 5 zoogeographic areas. To summarize, our findings provide valuable information on the distribution, tick-host associations, and zoogeographic division of ticks in Jiangxi Province, China. Their molecular characterizations, phylogenetic relationships, and tick-borne pathogens that they may transmit should be further explored.
- Published
- 2022
26. Formation mechanisms and supervisory prediction of scaling in water supply pipelines: A review
- Author
-
Chang-Geng, Li, Cheng, Liu, Wen-Hui, Xu, Ming-Gang, Shan, and Hai-Xia, Wu
- Subjects
Corrosion ,Environmental Engineering ,Metals ,Water Supply ,Water Quality ,Ecological Modeling ,Pollution ,Waste Management and Disposal ,Calcium Carbonate ,Water Science and Technology ,Civil and Structural Engineering - Abstract
The scaling problem in the water supply pipeline will increase the resistance coefficient of the pipeline and the pressure of the water supply pipeline, which will not only affect the operation safety of the water supply pipeline, but also cause energy waste. The scale in the pipeline will also enrich heavy metal ions and pathogenic microorganisms, affecting the safety of water supply water quality and causing secondary pollution of water quality. At present, a lot of research has been done on the composition structure and crystallization process of the scale. The study found that calcite is the main component of the scale; the scale process is a heterogeneous nucleation process induced by heavy metal particles and their corrosion products in the pipeline. The introduction of electrochemical detection technology, density functional theory and molecular dynamics simulation has greatly improved the accuracy and timeliness of water scaling conditions detection and realized the visualization of scaling mechanism. In this paper, the measurement methods of the scale in the water supply pipeline and the corresponding material composition and crystal structure characteristics are reviewed, and the mechanism of the scale and the water quality conditions are summarized. At the end of this paper, based on summarizing the existing water quality scaling tendency evaluation methods, it is proposed to establish a water quality potential scaling risk assessment framework based on Puckorius scaling index (PSI) and electrochemical impedance spectroscopy (EIS) in the future.
- Published
- 2022
27. Taxonomy and the evolutionary history of Micropeltidaceae
- Author
-
Xiang-Yu Zeng, Kevin D. Hyde, Ting-Chi Wen, Rajesh Jeewon, Sinang Hongsanan, Sajeewa S. N. Maharachchikumbura, Hai-Xia Wu, and Putarak Chomnunti
- Subjects
0106 biological sciences ,0303 health sciences ,Ecology ,Phylogenetic tree ,biology ,biology.organism_classification ,Crown group ,01 natural sciences ,Micropeltidaceae ,030308 mycology & parasitology ,03 medical and health sciences ,Herbarium ,Evolutionary biology ,Scolecopeltidium ,Taxonomy (biology) ,Molecular clock ,Ecology, Evolution, Behavior and Systematics ,010606 plant biology & botany ,Lecanoromycetes - Abstract
Micropeltidaceae species are flyspeck fungi which have been subjected to few systematic studies. We re-examined 27 genera which were accepted in the Micropeltidaceae and re-described them based on herbaria materials and protologues. Based on morphology and phylogenetic investigations, we transfer Micropeltidaceae to a new order, Micropeltidales (Lecanoromycetes). Genera with bluish or greenish upper walls (Dictyopeltella, Dictyothyriella, Dictyothyrina, Dictyothyrium, Haplopeltheca, Micropeltis, Scolecopeltidium and Stomiopeltopsis) are accepted in the new taxonomic concept for Micropeltidaceae. A molecular clock approach estimated the divergence time of the Micropeltidaceae crown group at 130 (165–104) Mya, which also supports its rank as an order (diverging from 220–100 Mya). The evolutionary histories between Micropeltidaceae species and host plants are interpreted by cophylogenetic analyses calibrated by their divergence times. The result indicates that the diversification of Angiospermae (130–80 Mya) fosters the formation of genera of Micropeltidaceae mainly via cospeciation events, and this codivergent period would be an important reference when establishing generic boundaries of epifoliar fungi.
- Published
- 2019
28. Type studies in Micropeltidaceae and suggested transfer of three excluded genera
- Author
-
Cheng-shun Yi, Ke Rong, Hang Chen, and Hai-Xia Wu
- Subjects
Evolutionary biology ,Taxonomy (biology) ,Plant Science ,Biology ,biology.organism_classification ,Ecology, Evolution, Behavior and Systematics ,Micropeltidaceae - Published
- 2019
29. Long Afterglow of a Nonporous Coordination Polymer with Tunable Room-Temperature Phosphorescence by the Doping of Dye Molecules
- Author
-
Xiao-Min Lu, Xiao-Gang Yang, Jia-Yi Chen, Lu-Fang Ma, Hai-Xia Wu, and Wen-Jing Qin
- Subjects
chemistry.chemical_classification ,010405 organic chemistry ,Chemistry ,Coordination polymer ,Doping ,Biasing ,Polymer ,Photoelectric effect ,010402 general chemistry ,Photochemistry ,01 natural sciences ,0104 chemical sciences ,Inorganic Chemistry ,chemistry.chemical_compound ,Molecule ,Physical and Theoretical Chemistry ,Phosphorescence ,Eosin Y - Abstract
Metal-organic frameworks (MOFs) or coordination polymers (CPs)-based phosphorescence materials may provide a powerful route for photoelectric and optical recording devices. Herein, two phosphorescence ligands, iso-phthalic acid (IPA) and 2-methylimidazole (MIM), were selected to construct an nonporous CP {Zn(IPA)(MIM)2} (1) with a long-lived phosphorescence lifetime up to 552 ms. By the doping of Eosin Y (EY) dye molecules under an in situ process, the phosphorescence emission color of 1 can be expressly tuned from green to red. The light-harvesting range can also be vastly broadened from the UV to the visible region (550 nm). Photoelectron measurements reveal that the synergistic effect of bias voltage and illumination can greatly restrain electron-hole recombination for the generation of additional free charges.
- Published
- 2021
30. Early Cumulus Cell Removal Increases Cumulative Live Birth Rate While Having No Negative Effect on the Malformation Rate in in Vitro Fertilization: A Propensity Score-Matched Cohort Study
- Author
-
Min Hao Liu, Mei Yuan Huang, Xiao Ming Teng, Jia Ping Pan, Hai Xia Wu, Li Juan Sun, and Shan Shan Liang
- Subjects
Cultural Studies ,History ,medicine.medical_specialty ,Public Administration ,Social Psychology ,Sociology and Political Science ,Visual Arts and Performing Arts ,Polymers and Plastics ,General Mathematics ,Strategy and Management ,medicine.medical_treatment ,Geography, Planning and Development ,Ocean Engineering ,Management, Monitoring, Policy and Law ,Development ,Industrial and Manufacturing Engineering ,Miscarriage ,Human fertilization ,History and Philosophy of Science ,Management of Technology and Innovation ,Architecture ,medicine ,Business and International Management ,Engineering (miscellaneous) ,General Environmental Science ,Civil and Structural Engineering ,In vitro fertilisation ,Obstetrics ,business.industry ,Communication ,General Social Sciences ,Embryo ,Polyspermy ,medicine.disease ,Urban Studies ,Philosophy ,Pregnancy rate ,Propensity score matching ,General Earth and Planetary Sciences ,General Agricultural and Biological Sciences ,Live birth ,business - Abstract
Background: Early cumulus cell removal (ECCR) in IVF may benefit from reducing potential detrimental exposure to free oxygen radicals (ROS) and other metabolic products which come from spermatozoa. While the other studies showed that EECR may be detrimental to embryo development and may break the important cross-talk between oocyte and cumulus cells during early embryos development. The effect of ECCR remains controversial to date. The aim of this study was to investigate the efficacy and safety of ECCR during human in vitro fertilization (IVF). Methods: A retrospective analysis was performed between January 2011 and December 2019. The study enrolled 1,131 couples who underwent IVF treatment with ECCR. After propensity score matching at a 1:1 ratio, 1,131 couples who underwent overnight coincubation of gametes were selected. The main outcome measure was the cumulative live birth rate. Secondary outcome measures included the cumulative pregnancy rate, polyspermy rate, available embryo rate, miscarriage rate, malformation rate, time to live birth, and oocyte-to-baby rate. Findings: There were no significant differences found between the two groups in the polyspermy rate, available embryo rate, miscarriage rate, time to live birth, oocyte-to-baby rate, and neonatal congenital anomalies rate. The results of the study showed that ECCR was associated with a significantly higher cumulative live birth rate and cumulative pregnancy rate, along with a significantly lower fertilization rate. Interpretation: ECCR tended to confer increased cumulative live birth rate and had no negative effect on the neonatal malformation rate. Funding Information: This work was supported by the Shanghai Public Health Bureau. Declaration of Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. Ethics Approval Statement: The protocol of this study was reviewed and approved by the Institutional Reviews Boards and Ethics Committee of Shanghai First Maternity and Infant Hospital, China on August 15, 2019 (reference number: 2019-57).
- Published
- 2021
31. Early cumulus cell removal reduces available embryo rate while having no negative effect on live-birth and malformation rate in IVF: a propensity score-matched cohort study
- Author
-
Min Hao Liu, Li Juan Sun, Jia Ping Pan, Shan Shan Liang, Mei Yuan Huang, and Hai Xia WU
- Abstract
Background Previous studies of the effect of early cumulus cell removal (ECCR) on clinical outcomes remain controversial. Some studies indicated that ECCR combined early rescue ICSI contributed to avoid total fertilization failure, while the other studies demonstrated that ECCR may be detrimental to early embryo development. The aim of this study is to investigate the efficacy and safety of early cumulus cell removal (ECCR) during human IVF. Methods A retrospective analysis was performed between January 2011 and December 2016. The study enrolled 655 couples who underwent IVF treatments with ECCR. After propensity score matching at a 1:2 ratio, 1310 couples who underwent overnight coincubation of gametes were selected. All data were obtained from the Shanghai First Maternity and Infant Hospital IVF patient database. The main outcome measure was the live birth rate and the secondary outcome measures were the normal fertilization rate, polyspermy rate, available embryo rate, clinical pregnancy rate, miscarriage rate and malformation rate. Results No significant differences were found in the live birth rate (28.55% vs 28.4%; RR of 1.008; 95% CI: 0.869-1.170; p=0.916), clinical pregnancy rate (48.28% vs 45.16%; RR of 1.069; 95% CI: 0.951-1.202; p=0.268), implantation rate (32.67% vs 33%; p=0.896), miscarriage rate (13.33% vs 9.32%; RR of 1.43; 95% CI: 0.916-2.232; p=0.115), neonatal congenital anomalies rate (1.32% vs 1.01%; RR of 1.306; 95% CI: 0.315-5.417; p=0.713) or birthweight between the two groups. The study showed that ECCR was associated with a significantly lower fertilization rate (73.86% vs 80.12%; p=0.000), normal fertilization rate (2PN)(62.76% vs 69%, p=0.000) and available embryo rate (59.62% vs 62.29%, p=0.001). There were no significant differences in the polyspermy rate (11.10% vs 11.11%, p=0.982) and cleavage rate (93.93% vs 93.50%, p=0.279) between the ECCR group and traditional insemination group. Conclusions ECCR tended to confer increased risk of a lower available embryo rate but had no negative effect on the live birth rate or the neonatal malformation rate.
- Published
- 2020
32. Diblock Polymer Brush (PHEAA-b-PFMA): Microphase Separation Behavior and Anti-Protein Adsorption Performance
- Author
-
Hai-Xia Wu, Chuanjun Liu, Lu-Fang Ma, Xiao-Hong Zhang, and Lin Huang
- Subjects
Silicon ,Materials science ,Surface Properties ,Dispersity ,Acrylic Resins ,02 engineering and technology ,010402 general chemistry ,Polymer brush ,01 natural sciences ,Contact angle ,Adsorption ,Polymethacrylic Acids ,Electrochemistry ,Copolymer ,Animals ,General Materials Science ,Spectroscopy ,chemistry.chemical_classification ,Atom-transfer radical-polymerization ,Fibrinogen ,Serum Albumin, Bovine ,Surfaces and Interfaces ,Polymer ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,0104 chemical sciences ,chemistry ,Chemical engineering ,Wettability ,Cattle ,Muramidase ,0210 nano-technology ,Protein adsorption - Abstract
In this paper, a series of amphiphilic diblock polymers of poly(hydroxyethylacrylamide)- b-poly(1H,1H-pentafluoropropyl methacrylate) (PHEAA- b-PFMA) were grafted from silicon wafer via surface-initiated atom transfer radical polymerization (SI-ATRP). Surface wettability and chemical compositions of the modified surfaces were characterized by contact angle goniometer and X-ray photoelectron spectroscopy (XPS) respectively. Molecular weight and polydispersity of each block were measured using gel permeation chromatography (GPC). The topography and the microphase separation behavior of PHEAA- b-PFMA surfaces were investigated by atomic force microscope (AFM). The results show that only when the grafting density (σ) and thickness of PHEAA brush were in the range of 0.9-1.3 (chain/nm2) and 6.6-15.1 nm, respectively, and the ratio of PFMA/PHEAA varied from 89/42 to 89/94, could the diblock copolymer phase separate into nanostructures. Further, the antiprotein adsorption performance of the modified surfaces against BSA, fibrinogen, and lysozyme was studied. The results indicated the modified surfaces could reduce the protein adsorption compared to the pristine silicon wafer. For Fibrinogen, the antiadsorption effect of PHEAA- b-PFMA-modified surfaces with microphase segregation was better than that of corresponding PHEAA modified surfaces. The results provide further evidence that surface composition and microphase segregation of fluorinated moieties of block copolymer brushes significantly impact protein adsorption behaviors.
- Published
- 2018
33. From Homogeneous to Heterogeneous: A Simple Approach to Prepare Polymer Brush Modified Surfaces for Anti-Adhesion of Bacteria
- Author
-
Xiao-Hong Zhang, Lin Huang, Chuan-Jun Liu, and Hai-Xia Wu
- Subjects
Materials science ,biology ,Brush ,02 engineering and technology ,Adhesion ,010402 general chemistry ,021001 nanoscience & nanotechnology ,Polymer brush ,Grafting ,biology.organism_classification ,01 natural sciences ,Surface energy ,0104 chemical sciences ,Surfaces, Coatings and Films ,law.invention ,Colloid and Surface Chemistry ,Chemical engineering ,Polymerization ,law ,Materials Chemistry ,Actinomyces naeslundii ,Wafer ,Physical and Theoretical Chemistry ,0210 nano-technology ,Biotechnology - Abstract
In this paper, the hydrophilic p(N-hydroxyethylacrylamide) (PHEAA) brush was grafted from silicon wafer, followed by the decoration of C3F7 groups with low surface energy to the side chains of PHEAA brush. The anti-adhesion performance of the modified surfaces was evaluated using Actinomyces naeslundii and Streptococcus mutans. For 4 h incubation, the silicon wafer with PHEAA brush (Si-PHEAA) was able to resist the adhesion of bacteria, while the anti-adhesion of the PHEAA-C3F7 decorated silicon wafer (Si-PHEAA-C3F7) had been improved. When the incubation time was extended to 24 h, the Si-PHEAA-C3F7 still exhibited excellent adhesion-repellent property against bacteria. And among the Si-PHEAA-C3F7 surfaces with 2.6%, 9% and 13% grafting degrees of C3F7, there was no obvious difference in anti-adhesion performance. The results indicated that the method of polymerization prior to decoration is easy to operate, moreover the prepared Si-PHEAA-C3F7 possessed the long-term and high-efficiency anti-adhesion of bacteria ability.
- Published
- 2018
34. Validity analysis of particles in the induced crystallization softening technology and optimization of the application mode
- Author
-
Tariq Mehmood, Chang-geng Li, Hai-xia Wu, and Cheng Liu
- Subjects
Materials science ,Terminal velocity ,Turbulence ,Process Chemistry and Technology ,Mixing (process engineering) ,law.invention ,law ,Water treatment ,Particle size ,Crystallization ,Composite material ,Safety, Risk, Reliability and Quality ,Waste Management and Disposal ,Effluent ,Softening ,Biotechnology - Abstract
Induced crystallization softening technology has been widely used in applied drinking water treatment projects. The application mode needs to be optimized to achieve the complete utilization of induced crystallization particles. Therefore, a series of experiments were conducted to evaluate the change in induced crystallization particles, water hardness removal and effluent characteristics. Subsequently, the main determinants and the corresponding optimal operating mode were discussed. The results showed an increase in particle size, reaction time, and a decrease in solution pH reduced the water hardness removal efficiency of reactor. More likely, bigger crystallization particles slow down the water turbulence, cause uneven mixing of softening agents, decline the solution pH, and eventually softening reaction stop. Until Ref / Rep decreases to 1, the particles will completely lose the ability to remove hardness. The initial particle size and the dosage of softening agents as well as accelerating the up-flow velocity employed in the system were found to be key factor for the time particles take to reach a critical size and terminal velocity that stops the reaction. Therefore, filling the smaller particles and gradually increasing the up-flow velocity of the system could prolong the lifetime of the particles in practical applications, improving the utilization efficiency of the unit volume. Furthermore, it optimizes the sand discharge cycle and produces safer waste residues.
- Published
- 2021
35. Research on Cultivation of Positive Occupational Emotions of Counselors in Colleges and Universities
- Author
-
Hai-xia Wu
- Published
- 2019
36. Fungal diversity notes 367–490: taxonomic and phylogenetic contributions to fungal taxa
- Author
-
Kevin D. Hyde, Sinang Hongsanan, Rajesh Jeewon, D. Jayarama Bhat, Eric H. C. McKenzie, E. B. Gareth Jones, Rungtiwa Phookamsak, Hiran A. Ariyawansa, Saranyaphat Boonmee, Qi Zhao, Faten Awad Abdel-Aziz, Mohamed A. Abdel-Wahab, Supharat Banmai, Putarak Chomnunti, Bao-Kai Cui, Dinushani A. Daranagama, Kanad Das, Monika C. Dayarathne, Nimali I. de Silva, Asha J. Dissanayake, Mingkwan Doilom, Anusha H. Ekanayaka, Tatiana Baptista Gibertoni, Aristóteles Góes-Neto, Shi-Ke Huang, Subashini C. Jayasiri, Ruvishika S. Jayawardena, Sirinapa Konta, Hyang Burm Lee, Wen-Jing Li, Chuan-Gen Lin, Jian-Kui Liu, Yong-Zhong Lu, Zong-Long Luo, Ishara S. Manawasinghe, Patinjareveettil Manimohan, Ausana Mapook, Tuula Niskanen, Chada Norphanphoun, Moslem Papizadeh, Rekhani H. Perera, Chayanard Phukhamsakda, Christian Richter, André L. C. M. de A. Santiago, E. Ricardo Drechsler-Santos, Indunil C. Senanayake, Kazuaki Tanaka, T. M. D. S. Tennakoon, Kasun M. Thambugala, Qing Tian, Saowaluck Tibpromma, Benjarong Thongbai, Alfredo Vizzini, Dhanushka N. Wanasinghe, Nalin N. Wijayawardene, Hai-Xia Wu, Jing Yang, Xiang-Yu Zeng, Huang Zhang, Jin-Feng Zhang, Timur S. Bulgakov, Erio Camporesi, Ali H. Bahkali, Mohammad A. Amoozegar, Lidia Silva Araujo-Neta, Joseph F. Ammirati, Abhishek Baghela, R. P. Bhatt, Dimitar Bojantchev, Bart Buyck, Gladstone Alves da Silva, Catarina Letícia Ferreira de Lima, Rafael José Vilela de Oliveira, Carlos Alberto Fragoso de Souza, Yu-Cheng Dai, Bálint Dima, Tham Thi Duong, Enrico Ercole, Fernando Mafalda-Freire, Aniket Ghosh, Akira Hashimoto, Sutakorn Kamolhan, Ji-Chuan Kang, Samantha C. Karunarathna, Paul M. Kirk, Ilkka Kytövuori, Angela Lantieri, Kare Liimatainen, Zuo-Yi Liu, Xing-Zhong Liu, Robert Lücking, Gianfranco Medardi, Peter E. Mortimer, Thi Thuong Thuong Nguyen, Itthayakorn Promputtha, K. N. Anil Raj, Mateus A. Reck, Saisamorn Lumyong, Seyed Abolhassan Shahzadeh-Fazeli, Marc Stadler, Mohammad Reza Soudi, Hong-Yan Su, Takumasa Takahashi, Narumon Tangthirasunun, Priyanka Uniyal, Yong Wang, Ting-Chi Wen, Jian-Chu Xu, Zhong-Kai Zhang, Yong-Chang Zhao, Jun-Liang Zhou, and Lin Zhu
- Subjects
0301 basic medicine ,03 medical and health sciences ,030104 developmental biology ,Ecology ,030108 mycology & parasitology ,Ecology, Evolution, Behavior and Systematics - Published
- 2016
37. VLSI implementation of an ultra-low-cost and low-power image compressor for wireless camera networks
- Author
-
Hai-Xia Wu, Jing Nie, Szu-Yin Lin, Tse-Yen Liu, Ting-Lan Lin, Shih-Lun Chen, Chih-Hsien Hsia, and Rih-Lung Chung
- Subjects
Very-large-scale integration ,business.industry ,Computer science ,ComputingMethodologies_IMAGEPROCESSINGANDCOMPUTERVISION ,020206 networking & telecommunications ,020207 software engineering ,02 engineering and technology ,computer.file_format ,Block Truncation Coding ,JPEG ,Computer graphics ,Embedded system ,0202 electrical engineering, electronic engineering, information engineering ,Wireless ,Bitmap ,business ,Gas compressor ,computer ,Computer hardware ,Information Systems ,Coding (social sciences) - Abstract
In this paper, a novel hardware-oriented color image compression algorithm based on digital halftoning and block truncation coding (BTC) techniques is proposed for very large-scale integration (VLSI) implementation. The proposed technique consists of a threshold generator, a bitmap generator, a BTC training module, a predictor, and a signed Golomb–Rice coding module. Because the wireless camera network is developed for perceiving, measuring, and collecting information from the environment, each camera sensing node should be designed to achieve low cost, small size, high compression rates, and low power consumption. Hence, a novel low-complexity, high-performance, transform-free, hardware-oriented color image compression algorithm based on digital halftoning and BTC is proposed. In order to achieve the performance of compressing images in real time, the proposed color image compression algorithm was realized by a VLSI technique. The VLSI architecture in this work contains only 8.1-k gate counts, and the core area is 81,000 μm2 synthesized using a TSMC 0.18-μm CMOS process. The operating frequency of this work is 100 MHz and consumes 2.91 mW, which is efficient to develop wireless electronic systems with the demand of color image compression in real time. Compared with JPEG- and JPEG-LS-based designs, this work reduces gate counts by at least 71.1 % and power consumption by 53 % and only requires a one-line-buffer memory.
- Published
- 2015
38. Recommended names for pleomorphic genera in Dothideomycetes
- Author
-
Eric W.A. Boehm, Johannes Z. Groenewald, Satinee Suetrong, Saranyaphat Boonmee, André Aptroot, Dhanushka N. Wanasinghe, Alan J. L. Phillips, Nalin N. Wijayawardene, Ying Zhang, Kevin D. Hyde, Dong-Qin Dai, Walter M. Jaklitsch, José Jailson Lima Bezerra, Saowaluck Tibpromma, Kazuaki Tanaka, David L. Hawksworth, J.H.C. Woudenberg, Margarita Hernández-Restrepo, Asha J. Dissanayake, Eric H. C. McKenzie, Indunil C. Senanayake, Jiye Yan, Hai-Xia Wu, Sinang Hongsanan, Huzefa A. Raja, Tao Yang, E. B. Gareth Jones, Rungtiwa Phookamsak, Erio Camporesi, Uwe Braun, Ruvishika S. Jayawardena, Jutamart Monkai, Amy Y. Rossman, James D. Lawrey, Jayarama D. Bhat, Pedro W. Crous, Putarak Chomnunti, Keith A. Seifert, Saowanee Wikee, Ausana Mapook, Li Wen Jing, Qing Tian, Melvina J. D’souza, Bernard Slippers, Joanne E. Taylor, Paul M. Kirk, and Kasun M. Thambugala
- Subjects
Teratosphaeria ,Ramularia ,Fusicoccum ,Phyllosticta ,biology ,Fungal systematics ,genera of fungi ,pleomorphism ,Dothideomycetes ,one fungus one name ,biology.organism_classification ,Agricultural and Biological Sciences (miscellaneous) ,Corynespora ,Article ,Venturia ,Botany ,Phaeosphaeria ,Ecology, Evolution, Behavior and Systematics - Abstract
This paper provides recommendations of one name for use among pleomorphic genera in Dothideomycetes by the Working Group on Dothideomycetes established under the auspices of the International Commission on the Taxonomy of Fungi (ICTF). A number of these generic names are proposed for protection because they do not have priority and/or the generic name selected for use is asexually typified. These include: Acrogenospora over Farlowiella; Alternaria over Allewia, Lewia, and Crivellia; Botryosphaeria over Fusicoccum; Camarosporula over Anthracostroma; Capnodium over Polychaeton; Cladosporium over Davidiella; Corynespora over Corynesporasca; Curvularia over Pseudocochliobolus; Elsinoë over Sphaceloma; Excipulariopsis over Kentingia; Exosporiella over Anomalemma; Exserohilum over Setosphaeria; Gemmamyces over Megaloseptoria; Kellermania over Planistromella; Kirschsteiniothelia over Dendryphiopsis; Lecanosticta over Eruptio; Paranectriella over Araneomyces; Phaeosphaeria over Phaeoseptoria; Phyllosticta over Guignardia; Podonectria over Tetracrium; Polythrincium over Cymadothea; Prosthemium over Pleomassaria; Ramularia over Mycosphaerella; Sphaerellopsis over Eudarluca; Sphaeropsis over Phaeobotryosphaeria; Stemphylium over Pleospora; Teratosphaeria over Kirramyces and Colletogloeopsis; Tetraploa over Tetraplosphaeria; Venturia over Fusicladium and Pollaccia; and Zeloasperisporium over Neomicrothyrium. Twenty new combinations are made: Acrogenospora carmichaeliana (Berk.) Rossman & Crous, Alternaria scrophulariae (Desm.) Rossman & Crous, Pyrenophora catenaria (Drechsler) Rossman & K.D. Hyde, P. dematioidea (Bubák & Wróbl.) Rossman & K.D. Hyde, P. fugax (Wallr.) Rossman & K.D. Hyde, P. nobleae (McKenzie & D. Matthews) Rossman & K.D. Hyde, P. triseptata (Drechsler) Rossman & K.D. Hyde, Schizothyrium cryptogamum (Batzer & Crous) Crous & Batzer, S. cylindricum (G.Y. Sun et al.) Crous & Batzer, S. emperorae (G.Y. Sun & L. Gao) Crous & Batzer, S. inaequale (G.Y. Sun & L. Gao) Crous & Batzer, S. musae (G.Y. Sun & L. Gao) Crous & Batzer, S. qianense (G.Y. Sun & Y.Q. Ma) Crous & Batzer, S. tardecrescens (Batzer & Crous) Crous & Batzer, S. wisconsinense (Batzer & Crous) Crous & Batzer, Teratosphaeria epicoccoides (Cooke & Massee) Rossman & W.C. Allen, Venturia catenospora (Butin) Rossman & Crous, V. convolvularum (Ondrej) Rossman & Crous, V. oleaginea (Castagne) Rossman & Crous, and V. phillyreae (Nicolas & Aggéry) Rossman & Crous, combs. nov. Three replacement names are also proposed: Pyrenophora grahamii Rossman & K.D. Hyde, Schizothyrium sunii Crous & Batzer, and Venturia barriae Rossman & Crous noms. nov.
- Published
- 2015
39. A new species of foliicolous lichenized fungi from southwest China: Calopadia ruiliensis sp. nov.
- Author
-
Hai-Xia WU, Wei-Bin REN, Xin-Hao LI, and Zi-Xiang YANG
- Subjects
- *
SPECIES , *FUNGI - Abstract
Calopadia ruiliensis, found on living leaves in southwest China, is described as a new species, based on morphology and phylogenetic analysis. It is characterized by a foliicolous thallus with yellow greenish to creamy yellow color, rounded apothecia with nonpruinose to thinly pruinose disc, a dark olive-green hypothecium and black-brown subhymenium, and 1-spored asci producing muriform ascospores. Phylogenetic analysis of LSU sequence data shows that the new species clusters in the genus Calopadia with high bootstrap support. The new species is compared with closely related and similar species of Calopadia and a comprehensive description and micrographs are provided. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
40. A randomized triple blind controlled trial comparing the live birth rate of IVF following brief incubation versus standard incubation of gametes
- Author
-
Ernest Hung Yu Ng, Jia Ping Pan, Yu Wang, Xiao Ming Teng, Mei Zhao, Hai Xia Wu, and zhi qin chen
- Subjects
Adult ,Male ,China ,Time Factors ,Fertilization in Vitro ,Insemination ,law.invention ,Miscarriage ,Andrology ,03 medical and health sciences ,0302 clinical medicine ,Randomized controlled trial ,law ,Pregnancy ,Outcome Assessment, Health Care ,Medicine ,Humans ,Advanced maternal age ,Birth Rate ,Incubation ,030219 obstetrics & reproductive medicine ,business.industry ,Rehabilitation ,Obstetrics and Gynecology ,medicine.disease ,Spermatozoa ,Reproductive Medicine ,Infertility ,Oocytes ,Workforce ,Gestation ,Female ,business ,Live birth ,Laboratories ,Live Birth - Abstract
Study question Does brief incubation of oocytes and spermatozoa improve the live birth rate (LBR) of IVF when compared with that of standard incubation? Summary answer Brief incubation of gametes does not improve the LBR of IVF when compared with standard incubation. What is known already Some small randomized studies showed that brief incubation was associated with a significantly higher ongoing pregnancy rate than standard incubation. Study design, size, duration This is a randomized triple blind study of 320 infertile women for their first or repeated cycles undergoing IVF between September 2015 and October 2016. Participants/materials, setting, methods Women were randomized into the brief incubation group (n = 160) or the standard incubation group (n = 160) according to a computer-generated randomization list. Oocytes were incubated with spermatozoa (0.3-1.2 million motile sperm/ml) for 3-4 h in the brief incubation group while oocytes were incubated with spermatozoa at similar concentration for 20 h in the standard incubation group. The primary outcome was the LBR (a baby born alive after 22 weeks gestation) in the fresh cycle. Main results and the role of chance There was no significant difference in the LBR between the brief and standard incubation groups based on both intention-to-treat [33.0% (53/160) versus 36.8% (59/160), relative risk (RR) 0.898 (95% CI = 0.666-1.212), P = 0.482] and per protocol [41.4%(53/128) versus 41.0% (59/144), RR1.011 (95% CI = 0.760-1.343), P = 0.942] analyses. Clinical pregnancy, ongoing pregnancy, miscarriage, multiple pregnancy and implantation rates were comparable for the two groups. Similar results were found with subgroup analysis of advanced maternal age, abnormal semen analysis and repeated IVF cycles. No differences were observed in cumulative LBR between two groups. Limitations, reasons for caution Various motile sperm concentrations of 0.3-1.2 million per ml were used for insemination and the reactive oxygen species level in the insemination medium was not measured. The highest level at 1.2 million per ml is still relatively low compared to prior studies, therefore we do not know whether brief incubation can improve the LBR using higher concentrations of spermatozoa. The present sample size may not be adequate to detect a smaller difference in the LBR. Wider implications of the findings The present study demonstrated that a brief incubation of gametes had no significant beneficial effect on the LBR when compared with the standard incubation. The practice of brief incubation of gametes is not necessary and this can save the already tight manpower in many laboratories. Study funding/competing interests The study was supported by the Merck-Serono China Research Fund for Fertility Experts (2015), which was not involved in study design, execution, data analysis and manuscript preparation. There are no conflicts of interest for all authors. Trial registration number ClinicalTrials.gov Identifier NCT02534857. Trial registration date 28 August 2015. Date of first patient’s enrolment 8 September 2015.
- Published
- 2018
41. Highly Efficient Antibacterial Surface Grafted with a Triclosan-Decorated Poly(N-Hydroxyethylacrylamide) Brush
- Author
-
Jian-Yun Xiao, Ren-Xi Zhuo, Lei Tan, Mei-Yan Yang, Chuanjun Liu, Zhao-Wen Tang, and Hai-Xia Wu
- Subjects
Materials science ,biology ,Surface Properties ,fungi ,Acrylic Resins ,Adhesion ,biology.organism_classification ,Polymer brush ,Streptococcus mutans ,Bacterial Adhesion ,Triclosan ,Anti-Bacterial Agents ,Contact angle ,chemistry.chemical_compound ,chemistry ,Attenuated total reflection ,Actinomyces naeslundii ,Actinomyces ,General Materials Science ,Composite material ,Bacteria ,Nuclear chemistry - Abstract
This work presented a highly efficient antibacterial Ti-surface which was grafted with poly(N-hydroxyethylacrylamide) (PHEAA) brush and further decorated with triclosan (TCS). The modified surfaces were characterized using contact angle measurements, X-ray photoelectron spectroscopy, and attenuated total reflectance Fourier transform infrared. The antibacterial performance of the modified surfaces was evaluated using the Streptococcus mutans and Actinomyces naeslundii attachment test. The Ti surface with PHEAA brush (Ti-PHEAA) was able to resist the adhesion of the bacteria, while the TCS-decorated Ti surface (Ti-TCS) showed the capability of killing the bacteria adhered on the surface. As we coupled the TCS to the PHEAA brush, the surface showed highly efficient antibacterial performance due to the combination of the resistance to the bacteria adhesion and its activity of killing bacteria.
- Published
- 2015
42. Glucose- and pH-Responsive Nanogated Ensemble Based on Polymeric Network Capped Mesoporous Silica
- Author
-
Zhao-Wen Tang, Ren-Xi Zhuo, Mei-Yan Yang, Lei Tan, Jian-Yun Xiao, Hai-Xia Wu, and Chuanjun Liu
- Subjects
Acrylate ,Materials science ,Polymers ,Atom-transfer radical-polymerization ,Inorganic chemistry ,Nanoparticle ,Hydrogen-Ion Concentration ,Mesoporous silica ,Silicon Dioxide ,Diffusion ,Nanopores ,chemistry.chemical_compound ,Hydrolysis ,Glucose ,Nanocapsules ,chemistry ,Delayed-Action Preparations ,Materials Testing ,Polymer chemistry ,General Materials Science ,Particle Size ,Phenylboronic acid ,Mesoporous material ,Porosity ,Acrylic acid - Abstract
In this paper, a glucose and pH-responsive release system based on polymeric network capped mesoporous silica nanoparticles (MSN) has been presented. The poly(acrylic acid) (PAA) brush on MSN was obtained through the surface-initiated atom transfer radical polymerization (SI-ATRP) of t-butyl acrylate and the subsequent hydrolysis of the ester bond. Then the PAA was glycosylated with glucosamine to obtain P(AA-AGA). To block the pore of silica, the P(AA-AGA) chains were cross-linked through the formation of boronate esters between 4,4-(ethylenedicarbamoyl)phenylboronic acid (EPBA) and the hydroxyl groups of P(AA-AGA). The boronate esters disassociated in the presence of glucose or in acidic conditions, which lead to opening of the mesoporous channels and the release of loaded guest molecules. The rate of release could be tuned by varying the pH or the concentration of glucose in the environment. The combination of two stimuli exhibited an obvious enhanced release capacity in mild acidic conditions (pH 6.0).
- Published
- 2015
43. Effects of acupotomylysis on basic fibroblast growth factor and CD34 levels in rabbits with third lumbar vertebral transverse foramen syndrome
- Author
-
Nai-Gang Liu, Chang-Qing Guo, Hong-Mei Sun, Hong Xu, Xiangri Li, Hai-Xia Wu, and Yingying Zhang
- Subjects
Muscle tissue ,Electroacupuncture ,medicine.medical_treatment ,Basic fibroblast growth factor ,CD34 ,Neovascularization, Physiologic ,Antigens, CD34 ,Lumbar vertebrae ,Revascularization ,chemistry.chemical_compound ,Lumbar ,Genetics ,medicine ,Foramen ,Animals ,Molecular Biology ,Lumbar Vertebrae ,business.industry ,Back Muscles ,Syndrome ,General Medicine ,Anatomy ,medicine.anatomical_structure ,chemistry ,Fibroblast Growth Factor 2 ,Rabbits ,business - Abstract
This study observed the local tissue homogenates in rabbits with third lumbar vertebral transverse foramen syndrome and explored the mechanism of acupotomylysis in local tissue revascularization. Thirty Japanese white rabbits were randomly divided into the following 5 groups of 6 rabbits each: normal, model, acupotomy, electroacupuncture (EA), and acupotomy-EA groups. All except the normal group were comprised of animal models of third lumbar vertebral transverse foramen syndrome prepared by embedding sponge in the left third lumbar transverse process. The rabbits in the acupotomy and EA groups underwent bilateral acupotomylysis intervention; those in the acupotomy-EA group underwent acupotomylysis and EA interventions. On the 28th day after modeling, the double-antibody ELISA was used to detect b-FGF and CD34 levels in the serum and homogenates of a muscle tissue sample from the left side of the third lumbar transverse process. The b-FGF levels in local muscle homogenates were significantly higher in the modeled rabbits than in the normal rabbits (P0.01), and the CD34 levels in the modeled group were significantly lower than in the normal group (P0.01). The b-FGF and CD34 levels in the EA, acutopomy, and acutopomy-EA groups were significantly lower than those in the modeled group (P0.01); the CD34 levels were significantly higher in the acupotomy-EA group than in the model group (P0.05); and the differences among the EA, acupotomy, and acupotomy-EA groups were not significant (P0.05). In conclusion, acupotomylysis regulates the levels of b-FGF and CD34 levels in serum and muscle tissue as well as local tissue revascularization.
- Published
- 2015
44. The dual-stimulated release of size-selected cargos from cyclodextrin-covered mesoporous silica nanoparticles
- Author
-
Lei Tan, Hai-Xia Wu, Chuanjun Liu, Ren-Xi Zhuo, and Mei-Yan Yang
- Subjects
chemistry.chemical_classification ,Cyclodextrin ,General Chemical Engineering ,Analytical chemistry ,Nanoparticle ,General Chemistry ,Mesoporous silica ,Fluorescence spectra ,Calcein ,chemistry.chemical_compound ,chemistry ,Ferrocene ,Chemical engineering ,Drug delivery ,Molecule - Abstract
A drug delivery system of dual-stimulated release of size-selected cargos from β-cyclodextrin-covered mesoporous silica nanoparticles was prepared. Calcein loaded mesoporous silica nanoparticles (MSN) were capped by β-cyclodextrin (β-CD) through photocleavable moieties to control its release. Then the small size cargo p-coumaric acid (CA) was loaded. The cavity of β-cyclodextrin was blocked by ferrocene through the host–guest interaction between the ferrocene and β-cyclodextrin. The small cargo can be released by the escape of ferrocene under +1.5 V electro-stimuli. Calcein molecules could not pass through the cavity of the β-cyclodextrin due to its bigger size. The calcein was released from the MSN after the detachment of the β-cyclodextrin cap from the MSN surface with UV irradiation. Fluorescence spectra demonstrate that different sized cargoes were released successfully step by step under external UV-light and electro stimuli respectively.
- Published
- 2015
45. Protein-resistance performance of amphiphilic copolymer brushes consisting of fluorinated polymers and polyacrylamide grafted from silicon surfaces
- Author
-
Ren-Xi Zhuo, Chuanjun Liu, Lei Tan, Hai-Xia Wu, and Mei-Yan Yang
- Subjects
chemistry.chemical_classification ,Materials science ,biology ,Atom-transfer radical-polymerization ,General Chemical Engineering ,General Chemistry ,Polymer ,Methacrylate ,Contact angle ,Adsorption ,chemistry ,Polymer chemistry ,Copolymer ,biology.protein ,Bovine serum albumin ,Protein adsorption - Abstract
A series of random copolymer brushes of acrylamide (AM) and 2-(perfluorinated hexyl)ethyl methacrylate (FMA) were grafted from initiator-functionalized silicon wafers by surface-initiated atom transfer radical polymerization. The water contact angle, X-ray photoelectron spectroscopy and atomic force microscopy were used to characterize surface wettability, surface composition, and morphology of the surfaces modified with polymer brushes, respectively. The protein-resistance performance of the surfaces grafted with polymer brushes was evaluated using micro-BCA protein assay reagent. It was found that the random copolymer brushes with the optimal ratio of AM to FMA in monomers showed the best protein-resistance performance, however, the optimal ratio is different for resisting bovine serum albumin and human plasma fibrinogen adsorption. For resisting bovine serum albumin adsorption, the optimal ratio of AM to FMA in monomers is 1 : 3, while the optimal is 3 : 1 for human plasma fibrinogen adsorption resistance. The results provide further evidence that surface compositional heterogeneities and microphase segregation of fluorinated moieties of amphiphilic random copolymer brushes significantly impact protein adsorption behaviors.
- Published
- 2015
46. Clinicopathological features in endometrial carcinoma with mismatch repair deficiency among Chinese women younger than 50 years of age
- Author
-
Yi-xin Liu, Shujie Pang, Jian-chan Song, Yi-quan Shi, Ommega Internationals, Hai-xia Wu, and Cheng Wang
- Subjects
Oncology ,medicine.medical_specialty ,business.industry ,Internal medicine ,medicine ,Carcinoma ,MISMATCH REPAIR DEFICIENCY ,Clinicopathological features ,business ,medicine.disease - Published
- 2015
47. Research on the Problem of Migrant Workers’ Rights Protection Predicament and Multi-dimensional Exclusion from the Capability Perspective
- Author
-
Xiao-wei Zhou, Shu-rong Han, and Hai-xia Wu
- Subjects
Empirical data ,Order (exchange) ,Migrant workers ,Political economy ,Perspective (graphical) ,Multi dimensional ,ComputingMilieux_LEGALASPECTSOFCOMPUTING ,Business ,Safeguarding ,Economic system ,Economic benefits - Abstract
In the decentralized economy, migrant workers, enterprises and local governments gradually differentiate into independent interest groups. In order to maximize economic benefits, enterprises and local governments may have negative effect on safeguarding the legitimate rights and interests of migrant workers. This paper uses empirical data to explain the difference between various interest groups has the resources and the resulting different capabilities under the capability perspective, which results in inferior status of migrant workers in the interests of the game to safeguard their legitimate rights and interests. There formal relief channels and passive way to achieve the purpose of safeguarding the interests of migrant workers to safeguard the interests. Based on the above, this paper puts forward the policy recommendations.
- Published
- 2017
48. Maleic Anhydride Grafted Modified PA6 as Compatilizer Preparation of PLA/PA6 Blend
- Author
-
Hai Rong Cui, Shui Qing Jiang, Jie Yuan Cui, and Hai Xia Wu
- Subjects
chemistry.chemical_compound ,Mechanical property ,Materials science ,chemistry ,Chemical engineering ,Maleic anhydride ,General Medicine - Abstract
This thesis is mainly about modifying PLA with PA6 as compatilizer and blending PLA with PA6. The alloying of PLA and PA6 can change the original fragility and mutability of PLA, and therefore improve its mechanical property. The blending of PLA into PA6 decreases the water absorbability and light resistance of PA6. Since PLA is of high cost, the blending of PA6 can lower the cost.
- Published
- 2014
49. PCSK6 regulated by LH inhibits the apoptosis of human granulosa cells via activin A and TGFβ2
- Author
-
Ying Wang, Li-Ping Jin, Ming-Qing Li, Hai-Xia Wu, Deng-Xuan Fan, Yuan Zhang, and Xiao-Hui Wang
- Subjects
Follistatin ,endocrine system ,medicine.medical_specialty ,Endocrinology, Diabetes and Metabolism ,Granulosa cell ,Apoptosis ,In Vitro Techniques ,Cell Line ,Transforming Growth Factor beta2 ,Aromatase ,Endocrinology ,Internal medicine ,medicine ,Humans ,Secretion ,RNA, Messenger ,Cells, Cultured ,Gene knockdown ,Granulosa Cells ,biology ,Serine Endopeptidases ,Estrogens ,Transforming growth factor beta ,Luteinizing Hormone ,Activins ,Up-Regulation ,biology.protein ,Receptors, FSH ,Female ,Proprotein Convertases ,Folliculogenesis ,Follicle-stimulating hormone receptor - Abstract
Mammalian proprotein convertases (PCs) play an important role in folliculogenesis, as they proteolytically activate a variety of substrates such as the transforming growth factor beta (TGFβ) superfamily. PC subtilism/kexin 6 (PCSK6) is a member of the PC family and is ubiquitously expressed and implicated in many physiological and pathological processes. However, in human granulosa cells, the expression of the PC family members, their hormonal regulation, and the function of PCs are not clear. In this study, we found that PCSK6 is the most highly expressed PC family member in granulosa cells. LH increasedPCSK6mRNA level and PCSK6 played an anti-apoptosis function in KGN cells. Knockdown ofPCSK6not only increased the secretion of activin A and TGFβ2 but also decreased the secretion of follistatin, estrogen, and the mRNA levels of FSH receptor (FSHR) andP450AROM(CYP19A1). We also found that, in the KGN human granulosa cell line, TGFβ2 and activin A could promote the apoptosis of KGN cells and LH could regulate the follistatin level. These data indicate that PCSK6, which is regulated by LH, is highly expressed in human primary granulosa cells of pre-ovulatory follicles and plays important roles in regulating a series of downstream molecules and apoptosis of KGN cells.
- Published
- 2014
50. Re-appraisal of Scolecopeltidium
- Author
-
Hai-Xia Wu and Kevin D. Hyde
- Subjects
biology ,Scolecopeltidium ,Engineering ethics ,Plant Science ,biology.organism_classification ,Ecology, Evolution, Behavior and Systematics - Published
- 2013
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.