Search

With the development of aerospace and space scientific research, satellite laser ranging (SLR) has put forward higher requirements for response speed, data density, and measurement accuracy. In coaxial common optical path laser ranging, the emitted laser and the received laser echoes pass through the same optical system. Due to the reversibility of the optical path, the laser emission, monitoring, and laser echoes’ optical path all pass through the same optical system structure, and the response speed and ranging ability of the laser ranging system have been greatly improved. Based on the SLR system of the Shanghai Astronomical Observatory (SHAO), the laser transmitting telescope with an aperture of 21 cm was used to build a polarized coaxial SLR system. It uses a picosecond pulsed laser with a pulse repetition frequency of 2 kHz and a single-pulse energy of 2 mJ. Also, a 4f system was applied to shrink the laser echo beam and filter out noise, the measurements of low-Earth orbit and long-distance high-orbit satellites were realized, and the ranging accuracy was ∼2 cm. As far as we know, this is currently the smallest aperture telescope for SLR globally, which is conducive to the miniaturization and integrated development of SLR systems.

An increasing attention is being given to treat fruits with ultraviolet C (UV-C) irradiation to extend shelf-life, senescence, and protection from different diseases during storage. However, the detailed understanding of the pathways and key changes in gene expression and metabolite accumulation related to UV-C treatments are yet to be explored. This study is a first attempt to understand such changes in banana peel irradiated with UV-C. We treated

Background: The three single nucleotide polymorphisms(SNPs) (rs4979356, rs4979357 and rs7868992) of COL27A1 has been reported to correlate with Tourette Syndrome (TS) patients in European countries as well as northern Chinese population, though there are lack of relevant studies in southern Chinese population. In this study we aimed to evaluate the distribution of COL27A1(rs4979356, rs4979357 and rs7868992) gene polymorphism and its clinical relationship in children with Tic disorder (TD) in southern China.Methods: The children with TD between November 2018 to August 2019 from Department of Neurology, Guangzhou Women and Children’s Medical Center were recruited and followed up. The control children from normal primary school were also recruited. The SNP of COL27A1 (rs4979356, rs4979357 and rs7868992) was detected in child from each groups by polymerase chain reaction(PCR) and Sanger sequencing.Results: A total of 114 children with TD and 100 healthy control children were included. 111 out of 114 TD’s children were followed up more than one year, including 32 with transient tic disorder (TTD) (Age:7.58±2.402;), 45 with chronic tic disorder (CTD) (Age:: 8.72±2.312;) and 34 with Tourette Syndrome (TS)(Age:: 8.85±2.720) respectively. The genotype frequency and gene frequency of rs4979356, rs4979357 and rs7868992 from COL27A1 showed no statistically significant difference (P > 0.05) between the both groups；The distribution of genotype CG of COL27A1 rs4979356 in TS group was significantly higher than that in TTD+CTD group( P=0.002) and in control group (P=0.001)；there was no significant difference in allele frequency among each groups (P＞0.05)；Genotype TC of COL27A1 rs4979357 was significantly more distributed in TS group than in TTD+CTD group (P=0.004) and control group (P=0.001)；There was no significant difference in allele frequency among three groups (P＞0.05);Genotype GA of COL27A1 rs7868992 was significantly more distributed in TS group than in TTD+CTD group (P=0.018) and control group (P=0.035). The distribution of G allele frequency in CTD group was significantly higher than that in TS group ( P=0.025), while the difference of other allele frequency between the groups was not statistically significant(P＞0.05).Conclusion: The genotype CG of rs4979356, the genotype TC of rs4979357 and the genotype GA of rs7868992 might serve as the high risk factors in children with TS in southern China.

Human leukocyte antigen-G (HLA-G) is a tumor-associated molecule, whose expression may help the cancer cells to escape the immune response.The aim of this study was to evaluate the diagnostic value of HLA-G level in oral squamous cell carcinoma (OSCC).A total of 52 patients who had definite pathological diagnosis and 20 cases of healthy controls were enrolled in this clinical trial. Immunohistochemisty (IHC) and quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR) analysis were considered for HLA-G identification and multilevel validations. Statistical analysis was performed using SPSS and statistical significance was determined at P0.05.IHC results demonstrated that the expression of HLA-G in OSCC was strongly positive and the rate of positive expression was 55.77% (29/52), but the expression of HLA-G in healthy controls was negative (0/20). Furthermore, RT-PCR results showed that the positive expression rate of HLA-G messenger RNA was weak in healthy controls, but strong in OSCC. Besides, HLA-G expression in the tumors was significantly correlated with histological grade.Our results suggested that HLA-G is associated with the prognosis of OSCC and may serve as a novel therapeutic target.

Surface modification of dental implants with biomolecules is of particularly interest recently. To mimic the structure and function of native extracellular matrix (ECM), a derivative of hyaluronic acid (HA), HA-GRGDSP, was synthesized, Arg-Gly-Asp (RGD)-containing collagen (Col)/HA multilayer polyelectrolyte films (MPFs) coating was fabricated on titanium (Ti) through alternate deposition of Col and HA-GRGDSP with 4.5 assembly cycles; moreover, bioactive molecule, basic fibroblast growth factor (bFGF), was also incorporated into such coating. This coating was then carefully characterized using scanning electronic microscope (SEM) and scanning force microscopy (SFM); bFGF release from the coating was also evaluated. (Col + bFGF)/HA-RGD coating was successfully deposited on Ti surface, and about 300 pg of bFGF could be slowly released from this coating for a week. This coating significantly promoted the initial cell attachment of human gingival fibroblasts (HGFs) compared with other groups (p < 0.05), and HGFs adhered and spread better on this coating than other groups (p < 0.05). Regarding cell proliferation and differentiation of HGFs, they were greatly stimulated when cultured on this coating (p < 0.05). These results indicated that surface modification of Ti using biomolecules might improve the sealing between the neck section of a dental implant and the soft tissue.

Summary Background Surface chemistry of dental implant plays an important role in osseointegration. Heat treatment might alter surface chemistry and result in different biological response. The aim of this study was to investigate the roles of heat treatment of H2O2/HCl-treated Ti implants in cell attachment, proliferation and osteoblastic differentiation. Material/Methods Sandblasted, dual acid-etched and H2O2/HCl heat-treated discs were set as the control group and sandblasted, dual acid-etched H2O2/HCl-treated discs were the test group. Both groups’ discs were sent for surface characterization. MC3T3-E1 cells were seeded on these 2 groups’ discs for 3 hours to 14 days, and then cell attachment, cell proliferation and cell differentiation were evaluated. Results Scanning electron microscope analysis revealed that the titanium discs in the 2 groups shared the same surface topography, while x-ray diffraction examination showed an anatase layer in the control group and titanium hydride diffractions in the test group. The cell attachment of the test group was equivalent to that of the control group. Cell proliferation was slightly stimulated at all time points in the control group, but the alkaline phosphatase (ALP) activity and osteocalcin (OC) production increased significantly in the test group compared with those in the control group at every time point investigated (p

To explore the development and prognosis of the acute flaccid paralysis (AFP) associated with enterovirus 71 (EV71) infection through clinical follow-up study for clinical and magnetic resonance imaging (MRI) features based on the research progress of virology and pathology.Sixteen children with HFMD associated with AFP in hospital from May 1, 2011 to August 31, 2011 were investigated and the patients received intensive rehabilitation training. The 16 cases were divided into two groups (the recovery or the sequela) by if the muscle strength recovered to level 4 after intensive rehabilitation. The MRI findings of 15 children were analyzed and among them, 6 patients were reexamined after one month. The clinical markers were compared between groups including course of disease, WBC, WBC in cerebrospinal fluid (CSF), ventilator support, therapy, the worst muscle strength, the initial tendon reflex, the muscle atrophy, and multi-limb paralysis. The data were analyzed by t test and χ2 test with SPSS10.0.All the 16 children were infected with enterovirus 71 (EV71). The myodynamia of 7 children were level 0, 4 children had serious upper limbs paralysis. The neck muscle in 3 cases and the brain stem motor ruckus in 4 cases were involved. The ankle clonus of non-completely paralyzed limbs in 14 cases occurred during rehabilitation. Eight children had the better prognosis, the other 8 children had sequela. 0 level muscle strength (0 case vs. 7 cases, χ2=12.4), the initial tendon reflex (2 cases vs. 8 cases, χ2=9.6), obvious muscle atrophy (0 case vs. 8 cases, χ2=16), were significantly different in the children with the recovery when compared to the sequela (P0.01). The severe upper limbs paralysis had the worse prognosis than the severe lower limbs paralysis. MR imaging showed signs of spinal nerve root inflammation and the bilateral hyperintense lesions, symmetrical in the posterior portions of the medulla, pons, and asymmetrical in the ventral horns of cervical spinal cord. Signal enhancement occurred only in the early MRI examination.In the evolution of AFP due to EV71 infection, the upper motor neuron damage is common, the prognosis is related with the severity of early paralysis and neuron damage. MR imaging is helpful to understand the pathological mechanism of AFP.

Objective There is no certain conclusion on the effect of recombinant human Osteogenic Protein-1 (OP-1, BMP-7) on the proliferation of the osteoblast-like cell line, MC3T3-E1. Furthermore, the optimal dose of rhOP-1 on cell differentiation still needs to be elucidated. This investigation aims to delineate the biofunctional characteristics of rhOP-1 in inducing osteoblastogenesis of MC3T3-E1 through in vitro time-course and dose–response studies. Design MC3T3-E1 cells were cultured for 1, 4, 7 days with the addition of different rhOP-1 concentrations (0, 10, 20, 50, 100, 200, 400 ng/ml), and cell proliferation and cell differentiation were examined. Results MC3T3-E1 cell proliferation was stimulated by rhOP-1 in a dose-dependent manner (0–400 ng/ml) on day 1, whereas on day 4 and 7, it was still stimulated at low concentrations (10, 20, 50 ng/ml) but inhibited at high ones (200, 400 ng/ml). The alkaline phosphatase (ALP) activity, osteocalcin (OC) production, collagen deposition and extracellular matrix mineralization were dramatically elevated by rhOP-1 treatment, as a function of culture time and rhOP-1 concentration, and all of them reached a plateau at the concentration of 200 ng/ml. Real-time quantitative RT-PCR results showed Runx2, AKP-2, OC and Nog mRNA expressions increased in a dose- and time-dependent manner, and their expressions were significantly higher at high rhOP-1 concentrations than that of low ones. No significant differences were found between the effects of 200 ng/ml rhOP-1 and 400 ng/ml rhOP-1 on the differentiation of MC3T3-E1 cells, except the expression of Nog mRNA, whose expression level was much higher at 400 ng/ml than that at 200 ng/ml. Conclusions These results suggest that cell proliferation of MC3T3-E1 is depended on culture time and rhOP-1 concentration, rhOP-1 could stimulate the differentiation of MC3T3-E1 cells and the optimal concentration could be 200 ng/ml.

The purpose of the present study was to evaluate the bioactivity of chemical treatment of titanium alloy (Ti-6Al-4V) in vitro. Smooth-surface discs of Ti-6Al-4V were used in this study. Sandblasted, dual acid-etched and H(2)O(2)/HCl heat-treated discs were set as test group, and sandblasted, dual acid-etched discs as control group. SEM and XRD analysis revealed a porous anatase gel layer on rough surface in the test group and a rough surface in the control group. Mouse pre-osteoblasts (MC3T3-E1 cells) were cultured on these 2 group discs, and then cell proliferation and differentiation were examined 4 days, 7 days, and 14 days after cell seeding. Cell proliferation was greatly stimulated at all time points when cultured in test group (P < .05). The alkaline phosphatase (ALP) activity and osteocalcin (OC) production were much higher in the test group compared with the control group at every time point investigated (P < .05). Furthermore, in the test group, the expressions of alkaline phosphatase-2, osteocalcin, and collagen type I alpha 1 mRNAs were significantly up-regulated as compared with those in the control group (P < .05 or P < .01). The results suggested that H(2)O(2)/HCl and heat-treatment might facilitate better integration of Ti-6Al-4V implants with bone.

The title complex, [Er(C5H5NO)(C2O4)(H2O)]n, is a new erbium polymer based on oxalate and 4-oxidopyridinium ligands. The ErII center is coordinated by six O atoms from three oxalate ligands, one O atom from a 4-oxidopyridinium ligand and one water mol­ecule, and displays a distorted square-anti­prismatic coordination geometry. The oxalate ligands are both chelating and bridging, and link ErII ions, forming Er–oxalate layers in which the attached water and 4-oxidopyridinium units point alternately up and down. A mirror plane passes through the Er atom, one C, the oxide O and two oxalate O atoms. The layers are assembled into a three-dimensional supra­molecular network via inter­molecular hydrogen bonding and π–π stacking inter­actions [centroid–centroid distances of 3.587 (2) Å between parallel pyridinium rings]. Both the water mol­ecule and the 4-oxidopyridinium ligand are disordered over two sites in a 1:1 ratio.

Corrigendum to Acta Cryst. (2008), E64, m649–m650., The chemical name in the title, the formula and the scheme of the paper by Gu, Hao, Song, Lin & Ma [Acta Cryst. (2008), E64, m649–m650] are corrected.