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The influences of superheat and cooling intensity on macrostructure and macrosegregation of one new kind duplex stainless steel (DSS) were studied. Thermal simulation equipment was applied to prepare samples, which could reproduce the industrial processes of DSS manufactured by a vertical continuous slab caster. Macrostructure and macrosegregation were analyzed using the digital single lens reflex and laser-induced breakdown spectroscope (LIBSOPA-200), respectively. The percentage of both chill zone and center equiaxed zone increases with the superheat decreasing, while that of the columnar zone decreases. There is only equiaxed grain existing as the superheat is 10 and 20 °C. The lower the superheat is, the coarser the gain size is. High cooling intensity in mold could remarkably decrease the chill zone length and refine the grains in chill zone and center equiaxed zone. The influences of cooling intensity on macrosegregation are greater than those of superheat. The macrosegregation of Si, Mn and Cr is slightly dependent on superheat, while that of Cu, Mo and Ni changes greatly with superheat increasing.

Catalyst free cyclocondensation of β-ethylthio-β-indolyl-α,β-unsaturated ketones with hydrazines has been developed, and 3-pyrazolyl indoles were efficiently synthesized in excellent yields. The catalyst free protocol avoids the use of a large excess of catalysts such as acids and bases, eliminating the discharge of harmful chemicals.

The Loschmidt echo is a useful diagnostic for the perfection of quantum time-reversal process and the sensitivity of quantum evolution to small perturbations. The main challenge for measuring the Loschmidt echo is the time reversal of a quantum evolution. In this work, we demonstrate the measurement of the Loschmidt echo in a superconducting 10-qubit system using Floquet engineering and discuss the imperfection of an initial Bell-state recovery arising from the next-nearest-neighbor (NNN) coupling present in the qubit device. Our results show that the Loschmidt echo is very sensitive to small perturbations during quantum-state evolution, in contrast to the quantities like qubit population that is often considered in the time-reversal experiment. These properties may be employed for the investigation of multiqubit system concerning many-body decoherence and entanglement, etc., especially when devices with reduced or vanishing NNN coupling are used.

Eco-friendly and practical methods for the complementary regioselective synthesis of 3-(1-substituted pyrazol-3-yl) indoles and 3-(1-substituted pyrazol-5-yl) indoles, from DBSA-mediated regioselective cyclocondensation reaction of β-ethyltho-β-indolyl-α, β-unsaturated ketones and monosubstituted hydrazines in water through simply varying the appropriate reaction way, had been developed. The methods not only efficiently avoided the use of organic solvent but also exhibited attractive characteristics such as operational simplicity, broad substrate scope, easy separation of products and ease of scale-up.

Stage III should be subclassified into Stage IIIA and IIIB in the American Joint Committee on Cancer (8th Edition) staging system for pancreatic cancer

A new and efficient protocol for the synthesis of 2-hydroxybenzophenone derivatives through [5C+1C] annulation of α-alkenoyl-α-aroyl ketene dithioacetals and nitroalkanes had been developed. In the presence of 1 equivalent of K2CO3 in DMF at 70 °C, a range of α-alkenoyl-α-aroyl ketene dithioacetals smoothly react with nitroalkanes to provide various 2-hydroxy benzophenone derivatives in excellent yields. The protocol featured mild reaction conditions, no the formation of by-products, excellent yield, no involving the problems of both chemoselectivity and regioselectivity and wide substrate scope.

In this paper, we wish to report an environment friendly synthetic method for β-ketothioesters from a dodecylbenzenesulfonic acid (DBSA)-catalyzed hydrolysis reaction of chain α-oxo ketene dithioacetals in water. It was shown that the hydrolysis reaction of chain α-oxo ketene dithioacetals could efficiently occur in the presence of 7.5 mol% DBSA at 100 °C in water, affording the desired β-keto thioesters in excellent yields.

A novel FeCl3·6H2O-catalyzed tandem Friedel–Crafts alkylation–hydrolysis reaction between chain α-oxo ketene dithioacetals and alcohols to afford α-alkylated β-oxo thioesters has been successfully developed. The reaction is efficient in the presence of catalyst loading as low as 30 mol% in MeCN at room temperature, and a wide variety of α-alkylated β-oxo thioesters are efficiently synthesized in good yields.

A novel long wavelength operating colorimetric and fluorescent chemosensor, Si-rhodamine B thiolactone (Si-RBS), has been successfully synthesized and characterized. Si-RBS demonstrated high selectivity towards Hg 2+ with about 670-fold enhancement in fluorescence emission intensity at 687 nm over various environmentally relevant metal cations such as Ag + , Al 3+ , Ca 2+ , Cd 2+ , Cr 3+ , Cu 2+ , Fe 3+ , K + , Mg 2+ , Mn 2+ , Ni 2+ , Pb 2+ and Zn 2+ in pH 5.2 media. The addition of Hg 2+ induced a significant change in color of Si-RBS solution from colorless to light blue, making the “naked-eye” recognition of mercury ions possible. Importantly, high sensitivity (detection limit down to 0.25 nM) and a rapid response time ( 2+ .

We aimed to examine the possible association between glycemic variability (GV) and all-cause mortality in patients with diabetes receiving hemodialysis.We conducted a prospective longitudinal cohort study of patients with diabetes mellitus undergoing hemodialysis at the Jiaxing Hemodialysis Center between December 2012 and December 2018. GV quantified with coefficient of variation (CV). The endpoint in the current study was defined as all-cause mortality.Of 1240 patients included in the analyses, there were 340 (27.4%) patients with death from all causes during a median follow-up time of 2.4 years (range 0-3.0). In a Kaplan-Meier analysis, cumulative survival was significantly dependent on CV quartiles with an apparent dose-response (log-rank P 0.001). In a multiple Cox regression model, the highest CV quartile was independently associated with increased risk of all-cause mortality (HR 1.887, 95% CI 1.407-2.531, P 0.001).Higher GV is associated with increased mortality risk among patients with diabetes receiving hemodialysis. Future studies are needed to explore whether decreasing GV would be associated with reduced risk of mortality.

An efficient and selective synthesis of 3-acetyl free(N-H)/N-substituded indoles and 3-acetoacetyl free(N-H)/N-substituded indoles has been developed via the hydrolysis reaction of β-ethylthio-β-indoly α, β-unsaturated ketones in the presence of 3 equivalent of NaOH and 5 mol% of H2SO4, respectively. The procedure features easy operation, excellent yields, and high selectivity, compatibility and practicability.

Anxiety is a frequent and serious complication of children and adolescents receiving dialysis. Low serum vitamin D levels have been associated with anxiety in non-pediatric patients. This study sought to examine the possible association between serum vitamin D levels and the presence of anxiety in children and adolescents with dialysis in China. A total of 156 pediatric patients who were on hemodialysis or peritoneal dialysis and 100 healthy controls were included in the current study. Serum 25-hydroxyvitamin D [25(OH)D] levels were measured by using a competitive protein-binding assay. Anxiety was assessed by using the Chinese version of the Screen for Child Anxiety Related Emotional Disorders (SCARED, = 25 as cutoff). Among 156 patients, 110 had a current anxiety (70.5%) and 46 did not (29.5%). Serum levels of 25(OH)D were significantly lower in patients with anxiety than in normal controls (19.4 ± 10.3 vs. 38.6 ± 15.5 ng/ml, P P = 0.003). Our research demonstrates that low serum levels of vitamin D are independently associated with anxiety among children and adolescents on dialysis, which needs to be confirmed in future experimental and clinical studies.

AIM To ascertain the prognostic role of the T4 and N2 category in stage III pancreatic cancer according to the 8th edition of the American Joint Committee on Cancer (AJCC) classification. METHODS Patients were collected from the Surveillance Epidemiology and End Results (SEER) database (2004-2013) and were divided into three groups: T(1-3)N2, T4N(0-1), and T4N2. Overall survival (OS) and disease-specific survival (DSS) of patients were evaluated by the Kaplan-Meier method. RESULTS For the first time, we found a significant difference in OS and DSS between T(1-3)N2/T4N(0-1) and T4N2 but not between T(1-3)N2 and T4N(0-1). A higher grading correlated with a worse prognosis in the T(1-3)N2 and T4N2 groups. CONCLUSION Patients with stage T4N2 had a worse prognosis than those with stage T(1-3)N2/T4N(0-1) in the 8th edition AJCC staging system for pancreatic cancer. We recommend that stage III should be subclassified into stage IIIA [T(1-3)N2/T4N(0-1)] and stage IIIB (T4N2).

Background The objective of this retrospective study was to determine whether lymph node metastasis has a prognostic impact on patients with stage IV breast cancer. Patients and methods Seven thousand three hundred and seventy-nine patients with de novo stage IV breast cancer diagnosed from 2004 to 2013 were identified. Kaplan-Meier estimate method was fitted to measure overall survival and breast cancer-specific survival (BCSS). Cox proportional hazard analysis was used to evaluate the association between N stage and BCSS after controlling variables such as other patient/tumor characteristics. Results The primary site of M1 tumors was mainly upper-outer quadrant and overlapping lesion of the breast. Patients with N1 disease had better overall survival and BCSS than did those without lymph node metastasis. The overall survival and BCSS of M1 patients with N3 disease were significantly lower than that of those with N0, N1 and N2 disease, whereas patients with N2 and N0/N1 involvement showed no significant difference with survival. Multivariate analysis showed that lymph node metastasis was an important prognostic factor for M1 patients (N1 versus N0, hazard ratio [HR] = 0.902, 95% confidence interval [CI]: 0.825-0.986, p = 0.023; N3 versus N0, HR = 1.161, 95% CI: 1.055-1.276, p = 0.002). For M1 patients, age, race, marital status, primary site, ER, PR and HER2 were the independent prognostic factors. Conclusions The cohort study provides an insight into de novo stage IV breast cancer with lymph node metastasis. Our results indicated that accurate lymph node evaluation for stage IV patients is still necessary to obtain important prognostic information.

Considering that the collision caused by hidden terminal is particularly serious due to the narrow beams of optical devices, the multi-packet reception (MPR) is introduced to mitigate the collisions for IEEE 802.15.7 visible light communication (VLC) system. To explore the impact of MPR on system performance and investigate the interaction between physical (PHY) layer and media access control (MAC) layer, a three dimensional (3D) integrated PHY-MAC analytical model of carrier sense multiple access/collision avoidance (CSMA/CA) is established based on Markov chain theory for VLC system, in which MPR is implemented through the use of orthogonal code sequence. Throughput is derived to evaluate the performance of VLC system with MPR capability under imperfect optical channel. The results can be used for the performance optimization of a VLC system with MPR capability.

Materials with the same elastic modulus E and representative stress and strain ( σ r , ɛ r ) present similar indentation–loading curves, whatever the value of strain hardening exponent n . Based on this definition, a good approach was proposed to extract the plastic properties or constitutive equations of metals from nanoindentation test combining finite element simulation. Firstly, without consideration of strain hardening, the representative stress was determined by varying assumed representative stress over a wide range until a good agreement was reached between the computed and experimental loading curves. Similarly, the corresponding representative strain was determined with different hypothetical values of strain hardening exponent in the range of 0–0.6. Through modulating assumed strain hardening exponent values to make the computed unloading curve coincide with that of the experiment, the real strain hardening exponent was acquired. Once the strain hardening exponent was determined, the initial yield stress σ y of metals could be obtained by the power law constitution. The validity of the proposed methodology was verified by three real metals: AISI 304 steel, Fe and Al alloy.

In order to investigate the seismic performance of zipper frames, three 10-storey structure models, including a zipper frame with tension zipper columns, a zipper frame with weak zipper columns and a chevron braced frame, were designed. Some indexes, including the natural periods, steel consumption and dynamic response under frequent and rare earthquakes, were analyzed and compared. The results show that, the dynamic responses of the three structures are similar to each other and all of them can meet the requirement of no-damage under frequent earthquakes and no-collapse under rare earthquakes provided by the GB50011 code, but the steel consumption for the zipper frames is less than that of the chevron braced frames, indicating the comprehensive seismic performance of the zipper frame is better than the chevron braced frame. However, it is also found that the design method of the zipper columns is conservative and a more reasonable design method of the zipper columns is needed to be provided.

Using α-oxo-ketene dithioacetals 1a as odorless thiol equivlents, an efficient and odorless transthioacetalization of acetals 2 has been developed. In the presence of MeCOCl in MeOH, the cleavage of 1a commences to generate thiols at both room and reflux temperatures, and the generated thiols then react with acetals 2 to give correspecting thioacetals 3 in good yield. This transthioacetalization is characterized by mild reaction conditions, simple procedure, good yields, and perfect chemoselectivity. It is noteworthy that only a very faint odor of thiols can be perceived during both the reaction and workup.

To assess the safety and efficacy of temporary ileostomy and temporary colostomy after a low anterior resection for rectal cancer by comparing the postoperative complications, then investigate which type of stoma is better.Studies comparing temporary ileostomy with colostomy for colorectal anastomosis were searched. The rates of complications (i.e., clinical anastomotic leak or fistula, stoma prolapse, parastomal hernia, wound infection related to stoma closure, obstruction following stoma closure, and skin trouble) were pooled and compared using a meta-analysis. The risk ratios (RRs) were calculated with 95% confidence intervals (CIs).The study included five randomized controlled trials (RCTs) and seven non-randomized studies involving 1687 patients. The meta-analysis of the RCTs demonstrated a lower risk of stoma prolapse (RR 0.15; 95% CI: 0.04-0.48) in the temporary ileostomy group. Meta-analysis of the non-randomized studies showed a lower risk of stoma prolapse and wound infection after stoma closure in the temporary ileostomy group (both p0.05).Temporary ileostomy has a minor impact on patients; we endorse temporary ileostomy over colostomy after a low anterior resection for rectal cancer.

A series of monoor bis-sulfonylthioureas has been synthesized via nucleophilic substitution of potassium sulfonamides and sodium dithiocarbamate in water under mild conditions, and their properties as anion receptors in the absence and presence of K + have been investigated by 1 H NMR spectroscopy. It was found that calix[4]crown-5 derivative 10 shows much higher selective complexation capability with H2PO4 in the presence of K + than in the absence of K + , which indicates the physical dimension of K + and H2PO4 , respectively, matched the corresponding cavity of the host compound.

Taking the botnet incident response plan decision-making as an example, we construct a new model by using action decomposition. In this model we introduce action layer, relation between sub-actions and decompose the complex incident response plan into many layers. This model provides a graphic representation of the complex plan. By analyzing the decomposing method and the action node structure, we prove that the store space size of this graphic representation is not increased exponentially when the action node added.

The aim of this study was to evaluate the association between activating enhancer binding protein 4 (AP-4) tissue expression and patient prognosis in hepatocellular carcinoma (HCC). The levels of AP-4 mRNA and protein in tumor and para-tumor tissue were evaluated in 30 HCC cases by quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot. Additionally, AP-4 protein expression in 112 HCC was analyzed by immunohistochemistry. The correlation of AP-4 expression and patients’ clinicopathological parameters was evaluated. Survival analysis was performed using the Kaplan–Meier method and Cox’s proportional hazards model. By RT-PCR and Western blot, the levels of AP-4 mRNA and protein were significantly higher in HCC, compared to that in para-tumor tissue (p

ADAM8 behaves as an active metalloprotease in vitro, hydrolyzing myelin basic protein and a variety of peptide substrates based on the cleavage sites of membrane-bound cytokines, growth factors, and receptors. Other studies have demonstrated overexpression of some ADAM family proteins in a variety of human tumors, but no report is available on the actual expression of ADAM8 and the correlation between clinicopathologic features and prognosis of hepatocellular carcinoma (HCC) patients. In this study, serum levels of ADAM8 were measured by ELISA in 126 patients with HCC, 50 patients with liver cirrhosis (LC), and 50 healthy individuals. The expression of ADAM8 in liver tissue was further studied using Western blotting in 126 patients with HCC and 50 with LC. The correlations between ADAM8 status and various clinicopathological parameters including survival were analyzed. Survival analysis was performed using the Kaplan-Meier method and Cox's proportional hazards model. The ELISA assay showed that the serum levels of ADAM8 in the HCC, LC, and healthy groups were 136.4 ± 34.5, 64.2 ± 20.1, and 63.2 ± 22.7 U/ml, respectively. Analysis of variance was used for inter-group comparison, and differences were found between the HCC group and the other two groups (both P < 0.001), while no difference was found between the LC group and the healthy group (P = 0.365). Western blotting assay showed that ADAM8 protein expression was detected in 62.7 % (79/126) HCC and in 32 % (16/50) LC tissues. Further, ADAM8 expression was associated closely with serum AFP elevation, tumor size, histological differentiation, tumor recurrence, tumor metastasis, and tumor stage. Kaplan-Meier survival analysis showed that patients with ADAM8-positive tumors had a shorter postoperative survival time than those with ADAM8-negative tumors (P < 0.001). Multivariate analysis revealed that ADAM8 expression was an independent prognostic parameter for the overall survival rate of HCC patients. These findings provide evidence that the expression of ADAM8 serves as a poor prognostic biomarker for HCC. ADAM8 may be a potential target of antiangiogenic therapy for HCC.

The aim of the study was to investigate the reactivations of hepatitis B virus (HBV) after rituximab-containing chemotherapy in patients with B-cell lymphoma with surface antigen of hepatitis B virus (HBsAg)-positive, or hepatitis B core antibody (HBcAb)-positive. A retrospective study of HBV-related markers was performed before and after rituximab-containing treatment in 189 consecutive patients with CD20-positive B-cell lymphoma. Among the 189 non-Hodgkin’s lymphoma (NHL) patients who received rituximab combination chemotherapy, 31 (16.6%) were HBsAg positive and 82 (43.9%) HBsAg negative/HBcAb positive, and 76 were HBsAg and HBcAb negative. Of the 31 HBsAg positive patients, 3 (9.7%) experienced reactivation of HBV. The prevalence of HBV reactivation was 4.0% (1/25) in patients who received prophylactic antiviral treatment and 33.3% (2/6) in those who did not receive prophylactic antiviral treatment (P = 0.032). Prophylactic antiviral treatment decreased the rate of HBV reactivation. Among the 82 HBsAg negative/HBcAb positive patients, 1 (1.2%) experienced HBV reactivation leading to serious hepatitis. Our experience indicates that rituximab-based therapy may cause serious HBV-related complications and even death in HBsAg-positive patients. Preemptive use of antiviral treatment enabled successful management of HBV reactivation. In HBsAg-negative and HBcAb-positive lymphoma patients the prevalence of HBV reactivation is low (1.2%). Close monitoring HBV until at least 6 months after anticancer therapy is required, prophylactic antiviral therapy needs to be evaluated further.

Material rolled in heavy plate mills and roughing stands often bends upwards or downwards. This phenomenon can lead to poor shape, profile and quality of the product, as well as lower productivity. This paper focuses on the well- known bending due to the related asymmetrical entrance angle between the rolling stock and work roll which can easily cause asymmetrical shape factor in deformation zone. A model for calculating curvature quickly is deduced based on the condition of asymmetrical entrance angle. Results show that the front end bending can occur easily due to the asymmetrical entrance angle. Moreover, the bending inclination of large reduction, large radius of roll and thin rolling stock is greater than that of the reverse; the bending inclination of the entrance thickness of rolling stock is greater than that of the reduction, and the bending inclination of the reduction is greater than that of the radius of roll.

Due to the complexity of the actual rolling process, asymmetrical conditions of deformation zone can cause front end bending or lead to poor shape, profile or quality of the product, as well as reducing productivity. Therefore it is important to study the geometric parameters of deformation zone in order to determine the work parameters accurately during rolling. Because the stock is often inclined when it enters the rolls, the deformation zone is asymmetrical. In this investigation, a model of calculating contact length between stock and roll precisely is deduced according to the theory of rolling. The results by theoretical calculation and experiment prove that the projected arc is raised while the stock enters the rolls serious asymmetrically, as well as the increase of the entrance thickness of the rolling stock and the decrease of the exit thickness of the rolled stock.

A formula of calculating curvature radius was deduced according to the theory of rolling, together with the characteristics of rolling process. Moreover, the simulation of producing process is represented. Simulating results indicate that the curvature radius could be reduced by increasing friction coefficient, friction coefficient ratio, reduction or roller radius, while it could be augmented by increasing the thickness of plate. Furthermore, increasing the thickness of plate would cause more effects on front end curvature, whereas reduction would do less. The result provides theoretic basis for eliminating the front end curvature in plate and sheet rolling process, and it is important to protect controlling equipment and reduce scrap as well.

3-[Bis(alkylthio)methylene]pentane-2,4-diones (1a and 1b) have been investigated as nonthiolic and odorless thiol equivalents for thia-Michael addition reactions under solvent-free conditions. Promoted by HCl (aq.), the cleavage of compounds 1 took place, and the in-situ generated thiols underwent facile conjugate addition to α?β-unsaturated carbonyl compounds 2 affording the corresponding ????????-keto sulfides 3 in high yields.

Systematic geochronologic, geochemical, and Nd isotopic analyses were carried out for an early Paleoproterozoic high-K intrusive complex exposed in southwestern Tarim, NW China. The results provide a better understanding of the Paleoproterozoic tectonic evolution of the Tarim Block. Zircon U–Pb age dating indicates two Paleoproterozoic magmatic episodes occurring at ca. 2.41 Ga and ca. 2.34 Ga respectively, which were followed by a ca. 1.9 Ga metamorphic event. The 2.41 Ga granodiorite–adamellite suite shares characteristics of late to post-orogenic metaluminous A-type granites in its high alkalinity (Na2O + K2O = 7.6–9.3%), total REE (410–788 ppm), Zr (370–660 ppm), and Y (21.7–58.4 ppm) contents. eNd(t) values for the suite range from − 3.22 to − 4.71 and accordingly the Nd modal ages (T2DM) vary between 3.05 Ga and 3.17 Ga. Based on geochemical data, the 2.34 Ga suite can be subdivided into two sub-suites, namely A-type and S-type. However, both types have comparable Nd isotope compositions (eNd(t) ≈ − 0.41 to − 2.08) and similar narrow T2DM ranges (2.76–2.91 Ga). Geochemical and Nd isotopic data for the high-K intrusive complex, in conjunction with the regional geological setting, suggest that both the 2.41 Ga suite and the 2.34 Ga A-type sub-suite might have been produced by partial melting of the Archean mafic crust in a continental rift environment. The S-type sub-suite is thought to have formed by partial melting of felsic pelites and/or metagreywackes recycled from Archean crust (TTG?). Gabbro enclaves with positive eNd(t) value (2.15) have been found to be intermingling within the 2.34 Ga suite; ca. 2.34–2.36 Ga gabbroic dykes and adamellites have previously been documented in eastern Tarim. These observations indicate that the high-K intrusions may reflect the emergence of depleted mantle upwelling beneath the Tarim Block at that time. We suggest a three-stages model for the Precambrian crustal evolution in the Tarim Block: (1) the formation of proto-crust (TTG) by ca. 2.5 Ga, (2) episodes of felsic magmatism possibly occurring in continental rift environments at ca. 2.41 Ga and ca. 2.34–2.36 Ga, and (3) ca. 1.9 Ga metamorphism that may represent the solidification of the Precambrian basement of the Tarim Block.

The synthesis of a zinc phthalocyanine (Pc) bearing four phenoxyl substituents was described and its molecular structure was fully characterized by 1 H NMR, MS, elemental analysis, UV–vis and IR, which were consistent with the proposed structure. This Pc possesses excellent solubility in polar organic solvents, such as dichloromethane and THF, and doesn't readily aggregate to the dimer. TOF-MS and elemental analysis prove its excellent purity. The compound's electrochemical redox reactions in THF occurred in the Pc ring and were quasi-reversible single-electron processes. The electrochemical reaction mechanism was proposed.

[Objective] To investigate the effect of cordyceps sinensis (CS) on expression of uncoupling protein-2 (UCP2) and so to elucidate the role of UCP2 in development of nonalcoholic fatty liver diseases (NAFLD). [Methods] Rats were administrated with high-fat diet to produce NAFLD animal model and intervened by cordyceps sinensis. Triglyceride (TG), total cholesterol (TC) in liver were measured by biochemistry, adenosine triphosphate (ATP) by luciferin-luciferinase, and UCP2 expression by Northern blotting and immunohistochemistry. Liver histopathology was evaluated. [Results] High-fat diet fed rats developed obesity and showed a gradual increase in body weight, liver index, and a decrease in ATP level. More advanced liver disease was found histopathologically for longer high-fat diet. Up-regulation of liver UCP2 by high-fat diet stopped after week 12. However codyceps sinensis induced UCP2 up-regulation continuously, and kept liver ATP a relatively high level. [Conclusion] NAFLD rat models were produced successfully. Liver UCP2 up-regulation in NAFLD rats may be a definite beneficial adaptation to lipid exposure. Cordyceps sinensis may serve a protective role to prevent NAFLD from progression. One of the possible mechanisms involves in modulating UCP2 expression and thereby, regulating fat metabolism, energy homeostasis.

Teraaminometallophthylocyanines (TAMPcs) 3a 1 – 3a 4 and 3b 1 – 3b 4 were prepared, in two steps, from 3-nitro-phthalic anhydride and 4-nitro- o -phenyleneimine, respectively. Although simple methods were employed without using chromatographic fractionation, purer products with higher yields were obtained. Synthesized compounds were characterized by MS, 1 H NMR, UV–VIS and IR. Characterization results showed that 3a 1 – 3a 4 contained intramolecular hydrogen bonds, while 3b 1 – 3b 4 did not contain intramolecular hydrogen bonds. Property comparisons between 3a 1 – 3a 4 and 3b 1 – 3b 4 are presented in this paper.

Although the enantioselectivity of the Pseudomonas cp. lipase (PCL) used in the kinetic resolution of ( R , S )-2-octanol is moderate, the ( S )-enantiomer 2 with 98% ee and ( R )-enantiomer 6 with 95% ee can be obtained by combining dynamic kinetic resolution (DyKR) with double kinetic resolution (DoKR) and 80% racemic substrate can be converted to enantiopure products by the way. The method is based on the feasibility of using the residue of one DoKR as the substrate of the next DoKR and so on recycling them to DyKR. The strategy is adapted to large-scale resolution of common sec -alcohols, especially to which when the moderate enantioselective lipases can be employed.

Rationale Ecthyma gangrenosum (EG) is an aggressive cutaneous disease caused by local or systemic infection with Pseudomonas aeruginosa. EG is characterized by cutaneous manifestations ranging from nodule and papule, to necrotic ulceration with surrounding erythema, especially with black eschar or central crust. EG presents with characteristic skin lesions which is important to establish diagnosis of sepsis caused by P aeruginosa, a serious condition that can be treated efficiently if diagnosed early. Patient concerns A 3-month-old female infant was presented with characteristic skin lesions of EG and developed sepsis 3 days later. Diagnoses Ecthyma gangrenosum and sepsis caused by Pseudomonas aeruginosa. Interventions Meropenem was used in combination with ceftazidime at first and excision of necrotic skin lesions was performed later. Outcomes Cure. Lessons Early recognition of EG plays an important role in providing appropriate empiric antibiotic treatment at early stage of sepsis, and improves the prognosis. Surgical excision may be helpful if no improvement was achieved via antibiotic treatment.

To investigate flora distribution and drug resistance in catheter-associated urinary tract infection. From January 2003 to January 2008 1,567 patients with urinary tract infection associated with the use of indwelling urinary catheters were analyzed retrospectively, whose urine specimens were cultured for bacteria and the isolated pathogens were tested for drug sensitivity by Kirby-Baue method. 376 pathogens were isolated from the 1,567 urine specimens (24·0%), most of which were Gram-negative bacteria. The extended spectrum β-lactamase (ESBL) rate was 59·8% (79/132) for Escherichia coli and 47·4% (18/38) for Klebsiella pnuemoniae. The isolating rate of Methcillin-resistant Staphylococcus aureus and Methcillin-resistant Staphylococcus epidermidis was 54·7% and 88·2% respectively. Catheter-associated urinary tract infection is mainly caused by Gram-negative bacteria of multi-drug resistance. Use of antibiotics should be based on drug sensitivity tests.

Background:Polycystic ovary syndrome (PCOS) is inconsistently associated with increased risk of adverse pregnancy outcomes. The purpose of this meta-analysis was to summarize the evidence regarding the strength of the association between pregnancy in women with PCOS and pregnancy complicatio

Using α-oxo-ketene dithioacetals 1a as odorless thiol equivlents, an efficient and odorless transthioacetalization of acetals 2 has been developed. In the presence of MeCOCl in MeOH, the cleavage of 1a commences to generate thiols at both room and reflux temperatures, and the generated thiols then react with acetals 2 to give correspecting thioacetals 3 in good yield. This transthioacetalization is characterized by mild reaction conditions, simple procedure, good yields, and perfect chemoselectivity. It is noteworthy that only a very faint odor of thiols can be perceived during both the reaction and workup.

To investigate the safety and feasibility of laparoscopic D2 gastrectomy for advanced gastric cancer.The clinical data of 210 cases of laparoscopic gastrectomy and 180 cases of open gastrectomy for radical (D2) gastrectomy from May 2007 to Dec 2010 were analyzed retrospectively.A total of 206 cases underwent laparoscopic-assisted surgery with 4 conversions. Compared to the open group, the laparoscopic group was associated with less bleeding [(208±38) ml vs. (300±52) ml, P0.05], quicker postoperative recovery of bowel function [(2.9±0.7) d vs. (3.9±1.8) d, P0.05], shorter postoperative length of hospital stay[(12.8±6.2) d vs. (15.6±6.8) d, P0.05], longer operative time [(258±42) min vs. (193±30) min, P0.05]. The number of lymph node harvested was 20.5±1.9 in the laparoscopic group and 25.8±1.5 in the open group, and the postoperative complication rate was 8.1% (17/201) vs. 8.5% (15/180), and differences were not statistically significant (both P0.05). The recurrence rate was 2.9% (6/210) and 2.8% (5/180), and the 3-year overall survival rate was 35.6% and 37.8%, the differences were not statistically significant (both P0.05).Laparoscopic radical gastrectomy for gastric cancer is safe and effective, which can reach the same range of lymph node dissection as open gastric cancer surgery and similar survival rate.

To investigate the expression and prognostic significance of RSF-1 in gastric adenocarcinoma.RSF-1 expression was analyzed using immunohistochemical staining on tissue samples from a consecutive series of 287 gastric adenocarcinoma patients who underwent tumor resections between 2003 and 2006.The relationship between RSF-1 expression, clinicopathological factors, and patient survival was investigated.Immunohistochemical staining indicated that RSF-1 is highly expressed in 52.6% of gastric adenocarcinomas. RSF-1 expression levels were closely associated with tumor size, histological differentiation, tumor stage, and lymph node involvement. Kaplan-Meier survival analysis showed that high RSF-1 expression exhibited a significant correlation with poor prognosis for gastric adenocarcinoma patients. Multivariate analysis revealed that RSF-1 expression is an independent prognostic parameter for the overall survival rate of gastric adenocarcinoma patients.Our data suggest that RSF-1 plays an important role in gastric adenocarcinoma progression and that high RSF-1 expression predicts an unfavorable prognosis in gastric adenocarcinoma patients.

To investigate the clinicopathological features and prognostic value of lysine specific demethylase 1 (LSD1) in hepatocellular carcinoma (HCC).We examined LSD1 expression in 60 paired liver cancer tissues and adjacent noncancerous tissues by quantitative real time polymerase chain reaction (qRT-PCR) and Western blotting. In addition, we analyzed LSD1 expression in 198 HCC samples by immunohistochemistry. The relationship between LSD1 expression, clinicopathological features and patient survival was investigated.Immunohistochemistry, Western blotting, and qRT-PCR consistently confirmed LSD1 overexpression in HCC tissues compared to adjacent non-neoplastic tissues (P0.01). Additionally, immunostaining showed more LSD1-positive cells in the higher tumor stage (T3-4) and tumor grade (G3) than in the lower tumor stage (T1-2, P0.001) and tumor grade (G1-2, P0.001), respectively. Moreover, HCC patients with high LSD1 expression had significantly lower 5-year overall survival rates (P0.001) and lower 5-year disease-free survival rates (P0.001), respectively. A Cox proportional hazards model further demonstrated that LSD1 over-expression was an independent predictor of poor prognosis for both 5-year disease-free survival [hazards ratio (HR) = 1.426, 95%CI: 0.672-2.146, P0.001] and 5-year overall survival (HR = 2.456, 95%CI: 1.234-3.932, P0.001) in HCC.Our data suggest for the first time that the overexpression of LSD1 protein in HCC tissues indicates tumor progression and predicts poor prognosis.

HOXA1 overexpression is sufficient for malignant transformation of nontumorigenic epithelial cells. It is known that HOXA1, which was upregulated in squamous cell carcinomas, affects both cell growth and death. The forced expression of HOXA1 in human breast cancer cells results in increased cell growth activity. However, it has not been reported in hepatocellular carcinoma (HCC). In this study, we used immunohistochemistry to compare HOXA1 protein expression in HCC and normal liver tissues and further analyzed HOXA1 protein expression in 156 clinicopathologically characterized HCC cases. We stably knocked down the endogenous expression level of HOXA1 in HepG2 cells with specific shRNA-expressing lentiviral vector. Following the successful establishment of stable cells, we examined in vitro cell growth by the MTT assay, anchorage-independent growth through a soft agar colony formation assay and cell migration/invasion by transwell and Boyden chamber assay. In addition, we also investigated in vivo tumor growth by xenograft transplantation of HepG2 cells into nude mice. Our results showed that the protein expression level of HOXA1 was markedly higher in HCC tissues than that in normal liver tissue (P = 0.019). In addition, a high expression level of HOXA1 protein was positively correlated with the T classification (P < 0.001), the N classification (P < 0.001), distant metastasis (P = 0.004), and the clinical stage (P < 0.001) of HCC patients. Patients with higher HOXA1 expression showed a significantly shorter overall survival time compared with patients with low HOXA1 expression. Multivariate analysis suggested that HOXA1 expression might be an independent prognostic indicator (P < 0.001) for the survival of patients with HCC. HOXA1-specific shRNA (shHOXA1) successfully knocked down HOXA1 endogenous expression in HepG2 cells. Compared to the parental and control shRNA-transfected (shCtrl) HepG2 cells, the shHOXA1 cells exhibited significantly reduced in vitro cell growth, anchorage-independent growth, and cell migration and invasion (P < 0.05). In vivo, the xenograft transplants from shHOXA1 cells gave rise to much smaller tumors compared with those from shCtrl cells. Collectively, high HOXA1 expression is associated with poor overall survival in patients with HCC. The downregulation of HOXA1 inhibits growth, anchorage-independent growth, and migration and invasion of HepG2 cells.