Search

Your search keyword '"Haga, Yuya"' showing total 43 results
Start Over Author "Haga, Yuya"
43 results on '"Haga, Yuya"'

15. Supplementary Table 1 from Host-Dependent Phenotypic Resistance to EGFR Tyrosine Kinase Inhibitors

Author

Haga, Yuya, primary, Marrocco, Ilaria, primary, Noronha, Ashish, primary, Uribe, Mary Luz, primary, Nataraj, Nishanth Belugali, primary, Sekar, Arunachalam, primary, Drago-Garcia, Diana, primary, Borgoni, Simone, primary, Lindzen, Moshit, primary, Giri, Suvendu, primary, Wiemann, Stefan, primary, Tsutsumi, Yasuo, primary, and Yarden, Yosef, primary

Published
2023
Full Text
View/download PDF

16. Supplementary Table 2 from Host-Dependent Phenotypic Resistance to EGFR Tyrosine Kinase Inhibitors

Author

Haga, Yuya, primary, Marrocco, Ilaria, primary, Noronha, Ashish, primary, Uribe, Mary Luz, primary, Nataraj, Nishanth Belugali, primary, Sekar, Arunachalam, primary, Drago-Garcia, Diana, primary, Borgoni, Simone, primary, Lindzen, Moshit, primary, Giri, Suvendu, primary, Wiemann, Stefan, primary, Tsutsumi, Yasuo, primary, and Yarden, Yosef, primary

Published
2023
Full Text
View/download PDF

17. Supplementary Table 3 from Host-Dependent Phenotypic Resistance to EGFR Tyrosine Kinase Inhibitors

Author

Haga, Yuya, primary, Marrocco, Ilaria, primary, Noronha, Ashish, primary, Uribe, Mary Luz, primary, Nataraj, Nishanth Belugali, primary, Sekar, Arunachalam, primary, Drago-Garcia, Diana, primary, Borgoni, Simone, primary, Lindzen, Moshit, primary, Giri, Suvendu, primary, Wiemann, Stefan, primary, Tsutsumi, Yasuo, primary, and Yarden, Yosef, primary

Published
2023
Full Text
View/download PDF

18. Supplementary Figures from Host-Dependent Phenotypic Resistance to EGFR Tyrosine Kinase Inhibitors

Author

Haga, Yuya, primary, Marrocco, Ilaria, primary, Noronha, Ashish, primary, Uribe, Mary Luz, primary, Nataraj, Nishanth Belugali, primary, Sekar, Arunachalam, primary, Drago-Garcia, Diana, primary, Borgoni, Simone, primary, Lindzen, Moshit, primary, Giri, Suvendu, primary, Wiemann, Stefan, primary, Tsutsumi, Yasuo, primary, and Yarden, Yosef, primary

Published
2023
Full Text
View/download PDF

19. Supplementary Information from Host-Dependent Phenotypic Resistance to EGFR Tyrosine Kinase Inhibitors

Author

Haga, Yuya, primary, Marrocco, Ilaria, primary, Noronha, Ashish, primary, Uribe, Mary Luz, primary, Nataraj, Nishanth Belugali, primary, Sekar, Arunachalam, primary, Drago-Garcia, Diana, primary, Borgoni, Simone, primary, Lindzen, Moshit, primary, Giri, Suvendu, primary, Wiemann, Stefan, primary, Tsutsumi, Yasuo, primary, and Yarden, Yosef, primary

Published
2023
Full Text
View/download PDF

23. Silver nanoparticles suppress forskolin-induced syncytialization in BeWo cells

Author

Sakahashi, Yuji, Higashisaka, Kazuma, Isaka, Ryo, Izutani, Rina, Seo, Jiwon, Furuta, Atsushi, Yamaki-Ushijima, Akemi, Tsujino, Hirofumi, Haga, Yuya, Nakashima, Akitoshi, and Tsutsumi, Yasuo

Abstract

Opportunities for the exposure of pregnant women to engineered nanoparticles have been increasing with the expanding use of these materials. Therefore, there are concerns that nanoparticles could have adverse effects on the establishment and maintenance of pregnancy. The effects of nanoparticles on the mother and fetus have been evaluated from this perspective, but there is still little knowledge about the effects on placentation and function acquisition, which are essential for the successful establishment and maintenance of pregnancy. Formation of the syncytiotrophoblast is indispensable for the acquisition of placental function, and impairment of syncytialization inevitably affects pregnancy outcomes. Here, we assessed the effect of nanoparticles on placental formation by using forskolin-treated BeWo cells, a typical in vitro model of trophoblast syncytialization. Immunofluorescence staining analysis revealed that silver nanoparticles with a diameter of 10 nm (nAg10) (at 0.156 µg/mL) significantly decreased the proportion of syncytialized BeWo cells, but gold nanoparticles with a diameter of 10 nm did not. Consistently, only nAg10 (at 0.156 µg/mL) significantly suppressed forskolin-induced elevation of CGB and SDC1 mRNA expression levels and human chorionic gonadotropin β production in a dose-dependent manner; these molecules are all markers of syncytialization. Besides, nAg10 significantly decreased the expression of ERVFRD-1, which encodes proteins associated with cell fusion. Moreover, nAg10 tended to suppress the expression of sFlt-1 e15a, a placental angiogenesis marker. Collectively, our data suggest that nAg10 could suppress formation of the syncytiotrophoblast and that induce placental dysfunction and the following poor pregnancy outcomes.

Published
2023
Full Text
View/download PDF

30. TSHZ2 is an EGF-regulated tumor suppressor that binds to the cytokinesis regulator PRC1 and inhibits metastasis

Author

Uribe, Mary L., primary, Dahlhoff, Maik, additional, Batra, Rajbir N., additional, Nataraj, Nishanth B., additional, Haga, Yuya, additional, Drago-Garcia, Diana, additional, Marrocco, Ilaria, additional, Sekar, Arunachalam, additional, Ghosh, Soma, additional, Vaknin, Itay, additional, Lebon, Sacha, additional, Kramarski, Lior, additional, Tsutsumi, Yasuo, additional, Choi, Inpyo, additional, Rueda, Oscar M., additional, Caldas, Carlos, additional, and Yarden, Yosef, additional

Published
2021
Full Text
View/download PDF

31. Host-Dependent Phenotypic Resistance to EGFR Tyrosine Kinase Inhibitors

Author

Haga, Yuya, primary, Marrocco, Ilaria, additional, Noronha, Ashish, additional, Uribe, Mary Luz, additional, Nataraj, Nishanth Belugali, additional, Sekar, Arunachalam, additional, Drago-Garcia, Diana, additional, Borgoni, Simone, additional, Lindzen, Moshit, additional, Giri, Suvendu, additional, Wiemann, Stefan, additional, Tsutsumi, Yasuo, additional, and Yarden, Yosef, additional

Published
2021
Full Text
View/download PDF

36. ETS Proteins Bind with Glucocorticoid Receptors: Relevance for Treatment of Ewing Sarcoma

Author

Srivastava, Swati, primary, Nataraj, Nishanth Belugali, additional, Sekar, Arunachalam, additional, Ghosh, Soma, additional, Bornstein, Chamutal, additional, Drago-Garcia, Diana, additional, Roth, Lee, additional, Romaniello, Donatella, additional, Marrocco, Ilaria, additional, David, Eyal, additional, Gilad, Yuval, additional, Lauriola, Mattia, additional, Rotkopf, Ron, additional, Kimchi, Adi, additional, Haga, Yuya, additional, Tsutsumi, Yasuo, additional, Mirabeau, Olivier, additional, Surdez, Didier, additional, Zinovyev, Andrei, additional, Delattre, Olivier, additional, Kovar, Heinrich, additional, Amit, Ido, additional, and Yarden, Yosef, additional

Published
2019
Full Text
View/download PDF

38. Coculture with macrophages alters ferroptosis susceptibility of triple-negative cancer cells

Author

Konishi, Hiroto, Haga, Yuya, Okumura, Moe, Tsujino, Hirofumi, Higashisaka, Kazuma, Tsutsumi, Yasuo, Konishi, Hiroto, Haga, Yuya, Okumura, Moe, Tsujino, Hirofumi, Higashisaka, Kazuma, and Tsutsumi, Yasuo

Abstract

Konishi H., Haga Y., Okumura M., et al. Coculture with macrophages alters ferroptosis susceptibility of triple-negative cancer cells. Cell Death Discovery 10, 108 (2024); https://doi.org/10.1038/s41420-024-01884-w., Various treatment options, such as molecular targeted drugs and immune checkpoint blockades, are available for patients with cancer. However, some cancer types are refractory to molecular targeted therapies or acquire drug resistance after long-term treatment. Thus, ferroptosis, a newly defined type of programmed cell death caused by the iron-dependent accumulation of lipid peroxidation, has gained attention as a novel cancer treatment strategy. Understanding cell–cell interactions in the tumor microenvironment is important for the clinical application of ferroptosis inducers. However, the effects of cell–cell interactions on ferroptosis sensitivity remain unclear. Thus, we aimed to evaluate the effects of macrophage–cancer cell interactions on ferroptosis induction. Coculture experiments showed that conditioned medium prepared from macrophages did not alter the ferroptosis sensitivity of cancer cells. By contrast, coculture via transwell, which enables cell–cell interactions through secretion, increased the sensitivity of cancer cells to ferroptosis inducers. Additionally, direct coculture increased the susceptibility of cancer cells to RSL3-induced ferroptosis. Mechanistically, coculture with macrophages upregulated the levels of intracellular ferrous ions and lipid peroxidation in cancer cells. These findings provide novel insights into the mechanisms by which cell–cell interactions influence ferroptosis induction and application of ferroptosis inducers as a cancer treatment option.

39. Polyethylene, whose surface has been modified by UV irradiation, induces cytotoxicity: A comparison with microplastics found in beaches

Author

Ikuno, Yudai, Tsujino, Hirofumi, Haga, Yuya, Manabe, Sota, Idehara, Wakaba, Hokaku, Mii, Asahara, Haruyasu, Higashisaka, Kazuma, Tsutsumi, Yasuo, Ikuno, Yudai, Tsujino, Hirofumi, Haga, Yuya, Manabe, Sota, Idehara, Wakaba, Hokaku, Mii, Asahara, Haruyasu, Higashisaka, Kazuma, and Tsutsumi, Yasuo

Abstract

Ikuno Y., Tsujino H., Haga Y., et al. Polyethylene, whose surface has been modified by UV irradiation, induces cytotoxicity: A comparison with microplastics found in beaches. Ecotoxicology and Environmental Safety 277, 116346 (2024); https://doi.org/10.1016/j.ecoenv.2024.116346., Microplastics, plastic particles 5 mm or less in size, are abundant in the environment; hence, the exposure of humans to microplastics is a great concern. Usually, the surface of microplastics found in the environment has undergone degradation by external factors such as ultraviolet rays and water waves. One of the characteristics of changes caused by surface degradation of microplastics is the introduction of oxygen-containing functional groups. Surface degradation alters the physicochemical properties of plastics, suggesting that the biological effects of environmentally degraded plastics may differ from those of pure plastics. However, the biological effects of plastics introduced with oxygen-containing functional groups through degradation are poorly elucidated owing to the lack of a plastic sample that imitates the degradation state of plastics found in the environment. In this study, we investigated the degradation state of microplastics collected from a beach. Next, we degraded a commercially available polyethylene (PE) particles via vacuum ultraviolet (VUV) irradiation and showed that chemical surface state of PE imitates that of microplastics in the environment. We evaluated the cytotoxic effects of degraded PE samples on immune and epithelial cell lines. We found that VUV irradiation was effective in degrading PE within a short period, and concentration-dependent cytotoxicity was induced by degraded PE in all cell lines. Our results indicate that the cytotoxic effect of PE on different cell types depends on the degree of microplastic degradation, which contributes to our understanding of the effects of PE microplastics on humans.

40. TSHZ2 is an EGF-regulated tumor suppressor that binds to the cytokinesis regulator PRC1 and inhibits metastasis

Author

Maik Dahlhoff, Sacha Lebon, Diana Drago-Garcia, Carlos Caldas, Ilaria Marrocco, R N Batra, Yosef Yarden, Inpyo Choi, Mary Luz Uribe, Yasuo Tsutsumi, Oscar M. Rueda, Yuya Haga, Soma Ghosh, Itay Vaknin, Arunachalam Sekar, Lior Kramarski, Nishanth Belugali Nataraj, Uribe, Mary L [0000-0002-6567-2436], Dahlhoff, Maik [0000-0001-9189-7631], Batra, Rajbir N [0000-0001-7010-8189], Nataraj, Nishanth B [0000-0003-2970-6739], Haga, Yuya [0000-0003-4148-2256], Drago-Garcia, Diana [0000-0001-6397-3123], Choi, Inpyo [0000-0003-2525-9695], Rueda, Oscar M [0000-0003-0008-4884], Caldas, Carlos [0000-0003-3547-1489], Yarden, Yosef [0000-0003-4168-7884], and Apollo - University of Cambridge Repository

Subjects
tumor suppressor, Breast Neoplasms, Cell Cycle Proteins, Biology, Biochemistry, Article, Mice, 03 medical and health sciences, 0302 clinical medicine, Epidermal growth factor, Animals, Humans, Genes, Tumor Suppressor, Breast, Cyclin B1, Molecular Biology, Mitosis, Transcription factor, Cytokinesis, 030304 developmental biology, Homeodomain Proteins, Settore BIO/11 - BIOLOGIA MOLECOLARE, 0303 health sciences, Epidermal Growth Factor, Kinase, Zinc finger homeobox 2, Cell Biology, 3. Good health, Cell biology, 030220 oncology & carcinogenesis, Female, PRC1
Abstract

Unlike early transcriptional responses to mitogens, later events are less well-characterized. Here, we identified delayed down-regulated genes (DDGs) in mammary cells after prolonged treatment with epidermal growth factor (EGF). The expression of these DDGs was low in mammary tumors and correlated with prognosis. The proteins encoded by several DDGs directly bind to and inactivate oncoproteins and might therefore act as tumor suppressors. The transcription factor teashirt zinc finger homeobox 2 (TSHZ2) is encoded by a DDG, and we found that overexpression of TSHZ2 inhibited tumor growth and metastasis and accelerated mammary gland development in mice. Although the gene TSHZ2 localizes to a locus (20q13.2) that is frequently amplified in breast cancer, we found that hypermethylation of its promoter correlated with down-regulation of TSHZ2 expression in patients. Yeast two-hybrid screens and protein-fragment complementation assays in mammalian cells indicated that TSHZ2 nucleated a multiprotein complex containing PRC1/Ase1, cyclin B1, and additional proteins that regulate cytokinesis. TSHZ2 increased the inhibitory phosphorylation of PRC1, a key driver of mitosis, mediated by cyclin-dependent kinases. Furthermore, similar to the tumor suppressive transcription factor p53, TSHZ2 inhibited transcription from the PRC1 promoter. By recognizing DDGs as a distinct group in the transcriptional response to EGF, our findings uncover a group of tumor suppressors and reveal a role for TSHZ2 in cell cycle regulation.

Published
2021

41. Orally Administered Silver Nanoparticles Are Absorbed and Migrate to Testes in Mice.

Author

Saeki Y, Higashisaka K, Izutani R, Seo J, Miyaji K, Haga Y, and Tsutsumi Y

Abstract

Given that daily exposure to nanoparticles is now unavoidable, there are concerns that nanoparticles have unexpected biological effects due to their small size. Here, we examined the biodistribution of silver nanoparticles, which are the most frequently used nanoparticles owing to their antibacterial activity, with a diameter of 10 nm (nAg10) to the male genital tract, and the effects of paternal treatment with nAg10 on fetal development. Male Slc:ICR male mice were orally treated with nAg10 for 14 consecutive days. Inductively coupled plasma mass spectrometry analysis detected silver in the blood and testis of male mice, but no general toxicological effects were induced. Moreover, there were no significant changes in fetal development when these treated male mice were mated with nontreated female mice. This implies that although orally ingested nAg10 is distributed to the male genital tract, it does not affect fetal development under the present treatment conditions., Competing Interests: The authors declare no competing financial interest., (© 2024 The Authors. Published by American Chemical Society.)

Published
2024
Full Text
View/download PDF

42. [Preparation of Degraded Microplastics That Imitate Surface Properties in the Environment].

Author

Tsujino H, Ikuno Y, Haga Y, Asahara H, Higashisaka K, and Tsutsumi Y

Subjects
Humans, Plastics, Environmental Monitoring, Polyethylene analysis, Surface Properties, Microplastics toxicity, Water Pollutants, Chemical analysis
Abstract

Microplastics are small pieces of plastic that are less than 5 mm in length. These plastics have been detected in various environments, including the ocean, soil, and air. Their abundance have raised concerns regarding their potential effects on living organisms, including humans. The surface of microplastics degrades due to external factors such as ultraviolet rays and water waves in the environment. Therefore, assessing the biological impact of microplastics and considering their state of degradation is important. Among the physical properties of microplastics, we focused on the chemical degradation of microplastics. Specifically, we used vacuum ultraviolet (VUV) light to accelerate the degradation of polyethylene (PE) and prepared PE samples representing the degradation of PE to varying degrees. The surface properties of PE samples prepared using VUV were similar to those obtained from the environment. Cytotoxicity tests were then used to evaluate the effects of undegraded and degraded PE on cells. We found that the severity of cytotoxicity increased with the extent to which the PE would have been degraded, suggesting that the degree of degradation is strongly linked to the severity of the observed deleterious effects on living organisms. In conclusion, this finding contributes to our understanding of the effects of polyethylene microplastics on the human body.

Published
2024
Full Text
View/download PDF

43. [Mechanisms of Cell Toxicity Caused by Degraded Microplastics].

Author

Haga Y, Manabe S, Tsujino H, Asahara H, Higashisaka K, and Tsutsumi Y

Subjects
Animals, Humans, Plastics toxicity, Ultraviolet Rays, Fishes, Microplastics toxicity, Water Pollutants, Chemical toxicity, Water Pollutants, Chemical analysis
Abstract

Microplastics (MPs), defined as plastic particles less than 5 mm in size, are ubiquitous in the environment. The accumulation of MPs in various environmental compartments, such as the ocean, soil, and air, has raised considerable concerns regarding their impact on ecological systems, including marine life and human health. Notably, MPs have been detected in marine organisms such as shellfish and fish, and have even been found in the human body, including in the blood and placenta. Moreover, considering that MPs have been detected in drinking water, human exposure to these particles in daily life is inevitable. To assess the risk posed by MPs to human health, it is essential to consider their physiological and chemical properties, including size, shape, surface modification, and material composition. However, current risk analyses focus primarily on spherical MPs with smooth surfaces, which differ substantially from most of the MPs detected in the environment. Environmental factors, such as ocean waves and ultraviolet radiation, alter the properties of MPs, including size, shape, and surface characteristics. In this review, we summarize current research on MPs, with a particular emphasis on the effects of MP degradation on human health. Furthermore, we generated MPs with surface degradation and evaluated their impact on cell toxicity, along with the underlying biological mechanisms.

Published
2024
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results