1,127 results on '"Haemophilus Infections diagnosis"'
Search Results
2. Haemophilus influenzae endocarditis: a case report and literature review.
- Author
-
Jaafar N, Duddu A, Guru S, and Oni I
- Subjects
- Humans, Female, Aged, 80 and over, Ampicillin therapeutic use, Tricuspid Valve microbiology, Tricuspid Valve diagnostic imaging, Haemophilus Infections drug therapy, Haemophilus Infections microbiology, Haemophilus Infections diagnosis, Haemophilus influenzae isolation & purification, Anti-Bacterial Agents therapeutic use, Endocarditis, Bacterial microbiology, Endocarditis, Bacterial drug therapy, Endocarditis, Bacterial diagnosis
- Abstract
Introduction: Haemophilus influenzae (HI) is an exceedingly rare cause of infective endocarditis (IE)., Case Presentation/methods: We present a case of a 90-year-old female diagnosed with HI-IE involving the native tricuspid valve in the absence of traditional risk factors for right-sided endocarditis. She was treated with a 5-week course of IV Ampicillin from negative cultures and suffered no complications. We also conducted a thorough literature review through PubMed and Google Scholar, which yielded a mere 15 reported cases of HI-IE., Results: Fourteen of the reported HI-IE cases included epidemiological data, showing no gender predominance. The mean age of the subjects was 39.5, with the mitral valve being the most implicated (64%) and tricuspid valve involvement being rare (21%)., Conclusion: Native tricuspid valve IE is an uncommon entity, especially in the absence of IV drug use. Haemophilus influenzae is an extremely rare cause of IE, with a literature review showing merely 15 reported cases. This article cites the 16th case of HI-IE published in the literature., (© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany.)
- Published
- 2024
- Full Text
- View/download PDF
3. Respiratory Pathogens at Exacerbation in Chronic Bronchitis With Airway Bacterial Colonisation: A Cohort Study.
- Author
-
Jones TL, Roberts C, Elliott S, Glaysher S, Green B, Shute JK, and Chauhan AJ
- Subjects
- Humans, Male, Female, Aged, Middle Aged, Bronchiectasis microbiology, Bronchiectasis complications, Cohort Studies, Disease Progression, Pseudomonas Infections microbiology, Pseudomonas Infections diagnosis, Pseudomonas Infections epidemiology, Pseudomonas Infections complications, Haemophilus Infections microbiology, Haemophilus Infections diagnosis, Haemophilus Infections complications, Haemophilus Infections drug therapy, Bronchitis, Chronic microbiology, Sputum microbiology, Haemophilus influenzae isolation & purification, Pseudomonas aeruginosa isolation & purification, Pulmonary Disease, Chronic Obstructive microbiology, Pulmonary Disease, Chronic Obstructive complications
- Abstract
Background and Objective: COPD and bronchiectasis are common causes of morbidity, particularly around exacerbation. Colonisation with respiratory pathogens can increase the frequency and severity of exacerbations. However, bacterial and viral presence at exacerbation in people with airway colonisation has not been well studied., Methods: A 6-month cohort study of participants (n = 30) with chronic bronchitis due to bronchiectasis (n = 26) and/or COPD (n = 13) and colonisation with Pseudomonas aeruginosa or Haemophilus influenzae was proven on two sputum cultures at exacerbation in the previous 12 months. Participants were provided self-management education and collected sputum samples daily. Sputum samples at baseline (at least 14 days before or after an exacerbation) and at each exacerbation were examined for a panel of 34 respiratory pathogens using commercially available RT-PCR kits and compared to results obtained using culture methods for the detection of bacteria., Results: Participants provided 29 baseline samples and 71 samples at exacerbation. In 17/29 baseline samples, RT-PCR analysis confirmed the organism demonstrated by culture, while 12 samples showed a discrepancy from culture results. Most exacerbations (57.7%) were not associated with acquiring new bacteria or viruses, while 19.8% showed new bacteria, 15.7% new viruses and 7% both new viruses and bacteria., Conclusion: Over half of exacerbations were not associated with new organisms in this cohort of participants with chronic bronchitis and colonisation. However, 26.8% demonstrated a new bacterial species in sputum, which is relevant for antibiotic therapy. Baseline RT-PCR and culture results were discordant in one-third of participants., (© 2024 The Author(s). The Clinical Respiratory Journal published by John Wiley & Sons Ltd.)
- Published
- 2024
- Full Text
- View/download PDF
4. Sequential development of diffuse panbronchiolitis and myasthenia gravis after thymectomy for thymic neoplasm: a case report.
- Author
-
Chou CY, Hsieh MS, and Kuo PH
- Subjects
- Humans, Female, Middle Aged, Tomography, X-Ray Computed, Haemophilus Infections etiology, Haemophilus Infections diagnosis, Thymoma surgery, Anti-Bacterial Agents therapeutic use, Taiwan, Myasthenia Gravis etiology, Bronchiolitis etiology, Thymectomy adverse effects, Thymus Neoplasms surgery, Thymus Neoplasms complications
- Abstract
Background: Myasthenia gravis (MG) is the most common paraneoplastic disorder associated with thymic neoplasms. MG can develop after thymectomy, and this condition is referred to post-thymectomy myasthenia gravis (PTMG). Diffuse panbronchiolitis (DPB), is a rare form of bronchiolitis and is largely restricted to East Asia, has been reported in association with thymic neoplasms. Only three cases of combined MG and DPB have been reported in the literature., Case Presentation: A 45-year-old Taiwanese woman presented to our hospital with productive cough, rhinorrhea, anosmia, ear fullness, shortness of breath, and weight loss. She had a history of thymoma, and she underwent thymectomy with adjuvant radiotherapy 7 years ago. Chest computed tomography scan revealed diffuse bronchitis and bronchiolitis. DPB was confirmed after video-assisted thoracoscopic surgery lung biopsy, and repeated sputum cultures grew Pseudomonas aeruginosa. She has been on long-term oral azithromycin therapy thereafter. Intravenous antipseudomonal antibiotics, inhaled amikacin, as well as oral levofloxacin were administered. Three months after DPB diagnosis, she developed ptosis, muscle weakness, and hypercapnia requiring the use of noninvasive positive pressure ventilation. MG was diagnosed based on the acetylcholine receptor antibody and repetitive stimulation test results. Her muscle weakness gradually improved after pyridostigmine and corticosteroid therapies. Oral corticosteroids could be tapered off ten months after the diagnosis of MG. She is currently maintained on azithromycin, pyridostigmine, and inhaled amikacin therapies, with intravenous antibiotics administered occasionally during hospitalizations for respiratory infections., Conclusions: To our knowledge, this might be the first case report of sequential development of DPB followed by PTMG. The coexistence of these two disorders poses a therapeutic challenge for balancing infection control for DPB and immunosuppressant therapies for MG., (© 2024. The Author(s).)
- Published
- 2024
- Full Text
- View/download PDF
5. Case of Haemophilus influenzae urethritis in a male patient.
- Author
-
Samra RS, Plummer EL, Vodstrcil L, and Bradshaw CS
- Subjects
- Humans, Male, Anti-Bacterial Agents therapeutic use, Haemophilus influenzae, Haemophilus Infections diagnosis, Haemophilus Infections drug therapy, Urethritis diagnosis, Urethritis drug therapy
- Abstract
Competing Interests: Competing interests: None declared.
- Published
- 2024
- Full Text
- View/download PDF
6. Haemophilus parainfluenzae infective endocarditis complicated by multiorgan septic emboli.
- Author
-
Bowden J, Hunt M, Harhay J, Patel T, and Thomas-Gosain N
- Subjects
- Male, Humans, Haemophilus parainfluenzae, Echocardiography, Haemophilus Infections complications, Haemophilus Infections diagnosis, Haemophilus Infections drug therapy, Endocarditis, Bacterial complications, Endocarditis, Bacterial diagnosis, Endocarditis, Bacterial drug therapy, Endocarditis microbiology
- Abstract
Infective endocarditis (IE) caused by Haemophilus parainfluenzae is a rare but serious condition if not diagnosed and treated promptly. In this article, we describe a patient with H. parainfluenzae IE who initially presented with non-specific symptoms but subsequently developed multiple sequelae of IE. The diagnosis of IE was made based on clinical, echocardiographic, radiological and microbiological findings. He was treated successfully with a mitral valve replacement along with 4 weeks of intravenous antibiotic therapy. Our case highlights the importance of obtaining a thorough history and a complete physical examination to ensure an early diagnosis of IE., Competing Interests: Competing interests: None declared., (© BMJ Publishing Group Limited 2024. No commercial re-use. See rights and permissions. Published by BMJ.)
- Published
- 2024
- Full Text
- View/download PDF
7. Acute Emphysematous Epiglottitis: A Case Report.
- Author
-
Sunyecz I, Orabi N, and Coutras S
- Subjects
- Child, Adult, Humans, Abscess complications, Acute Disease, Incidence, Haemophilus Infections complications, Haemophilus Infections diagnosis, Epiglottitis complications, Epiglottitis diagnosis, Epiglottitis therapy
- Abstract
Epiglottitis is a bacterial infection of the upper respiratory tract that can be rapidly progressive and life-threatening. Though predominantly seen in unvaccinated children, there seems to be a shift with the incidence of adult cases rising following the Haemophilus Influenza B (HiB) vaccine. There are several reports of epiglottitis manifesting as an abscess, but few cases report on the formation of an emphysematous abscess. Additionally, little is known on the bacterial etiology of such infections. Here, we present a case of a patient found to have acute emphysematous epiglottis managed with fiberoptic intubation, drainage, and culture of the abscess. Laryngoscope, 133:2747-2750, 2023., (© 2023 The Authors. The Laryngoscope published by Wiley Periodicals LLC on behalf of The American Laryngological, Rhinological and Otological Society, Inc.)
- Published
- 2023
- Full Text
- View/download PDF
8. Septic Arthritis Caused by Haemophilus parainfluenzae : A Pediatric Case Report and Literature Review.
- Author
-
Fukayama H, Myojin S, Funaki T, Fukuda Y, Nakamura T, Ishiguro A, and Ogimi C
- Subjects
- Male, Humans, Child, Child, Preschool, Haemophilus parainfluenzae, Risk Factors, Haemophilus Infections diagnosis, Haemophilus Infections drug therapy, Arthritis, Infectious diagnosis, Arthritis, Infectious drug therapy
- Abstract
We report a healthy 5-year-old boy without apparent risk factors who developed septic arthritis of the hip from Haemohilus parainfluenzae infection. A literature review revealed only 4 pediatric cases of osteoarticular infection caused by this pathogen. To our knowledge, our case may be the first pediatric case of septic arthritis of the hip caused by H. parainfluenzae ., Competing Interests: The authors have no conflicts of interest to disclose., (Copyright © 2023 Wolters Kluwer Health, Inc. All rights reserved.)
- Published
- 2023
- Full Text
- View/download PDF
9. Haemophilus influenzae septicaemia and urinary tract infection associated with nefrocalcinosis: case report.
- Author
-
Mathiasen ASF, Bigum LH, Andreassen KH, Slotved HC, Berring DCA, and Antsupova V
- Subjects
- Humans, Haemophilus influenzae, Haemophilus Infections complications, Haemophilus Infections diagnosis, Haemophilus Infections drug therapy, Bacteremia complications, Bacteremia diagnosis, Bacteremia drug therapy, Sepsis diagnosis, Urinary Tract Infections complications, Urinary Tract Infections diagnosis
- Abstract
Haemophilus influenzae is an uncommon uropathogen with fastidious growth requirements, which must be taken into consideration in the diagnostic process. We present a rare case of urosepsis with H. influenzae in a young patient with nefrocalcinosis., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.)
- Published
- 2023
- Full Text
- View/download PDF
10. A case of diffuse panbronchiolitis caused by Mycoplasma amphoriforme.
- Author
-
Green OJ, Ganim RB, and Mueller JD
- Subjects
- Humans, Mycoplasma, Bronchiolitis complications, Bronchiolitis diagnosis, Bronchiolitis drug therapy, Haemophilus Infections diagnosis, Haemophilus Infections drug therapy
- Abstract
Mycoplasma amphoriforme is a novel specie which was discovered in 2003 and associated with congenital immune deficiency. It has been described as an underlying cause of bronchopneumonia. There is limited description of the in vitro sensitivities. In this article, we present the first description of M. amphoriforme as the causative agent of diffuse panbronchiolitis in a patient with X-linked hypogammaglobulinema and bronchiectasis, with symptoms improved by treatment with azithromycin. We also describe the difficulty obtaining this organism through routine culture and the need to consider culture independent methods of recovery when the suspicion is high., Competing Interests: Declaration of Competing Interest No competing interests., (Copyright © 2023 Elsevier Inc. All rights reserved.)
- Published
- 2023
- Full Text
- View/download PDF
11. Haemophilus influenzae Infections.
- Author
-
Contreras A and Posada R
- Subjects
- Humans, Haemophilus Infections diagnosis
- Published
- 2023
- Full Text
- View/download PDF
12. Sudden death of a child associated with invasive non-typeable Haemophilus influenzae infection with underlying IgG 2 subclass deficiency.
- Author
-
Abe Y, Takano C, Tie J, Isobe E, Ohirabaru A, Isahai I, Nishiyama H, Jike T, Masuda S, and Okuda T
- Subjects
- Child, Preschool, Humans, Male, Death, Sudden etiology, Haemophilus Infections diagnosis, Haemophilus influenzae isolation & purification, IgG Deficiency blood, IgG Deficiency diagnosis
- Abstract
Haemophilus influenzae can be divided into typeable and non-typeable strains. Although non-typeable Haemophilus influenzae (NTHi) is less likely to be a fatal bacterium, invasive NTHi infection has been reported to increase worldwide. This study presents a case of sudden death of a child with invasive NTHi infection and underlying immunoglobulin G2 (IgG
2 ) deficiency. A two years seven months male child with a high fever was found unresponsive in bed, lying face down on a soft pillow. Later, the hospital declared the subject dead. An autopsy revealed that the only noteworthy finding was tissue congestion. The histopathological findings disclosed neutrophils within blood vessels of major organs. Meanwhile, the formation of the micro abscess was not visible, which indicated bacteremia. The bacterial blood culture was positive for Haemophilus Influenzae. Polymerase chain reaction assay revealed the absence of an entire capsule locus. The transmission electron microscopy showed that the colonies did not have polysaccharide capsules. Based on the above findings, the strain was identified as NTHi. Furthermore, the value of serum IgG2 was deficient, indicating the presence of IgG2 subclass deficiency. The subject eventually died from asphyxia by smothering due to a comorbid condition with a high fever brought on by NTHi-induced bacteremia and lying face down. IgG2 subclass deficiency contributed to the development of invasive NTHi infection. The invasive NTHi infection might present a risk of sudden death, particularly for immunocompromised children. As forensic pathologists and pediatricians may encounter such a problematic clinical condition, they should be aware of this., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier B.V. All rights reserved.)- Published
- 2023
- Full Text
- View/download PDF
13. Clinical characteristics of 32 cases of diffuse panbronchiolitis.
- Author
-
Zhang L, Ou Y, Hu H, Shen C, Cao Y, Chen Z, and Ouyang R
- Subjects
- Humans, Male, Female, Middle Aged, Aged, Lung diagnostic imaging, Macrolides therapeutic use, Anti-Bacterial Agents therapeutic use, Bronchiolitis diagnosis, Bronchiolitis drug therapy, Haemophilus Infections diagnosis, Haemophilus Infections drug therapy
- Abstract
Objectives: Diffuse panbronchiolitis (DPB) is a chronic airway inflammation with low specificity and its diagnosis is often missed or delayed. This study aims to summarize the clinical characteristics and treatment of DPB in order to improve the understanding and diagnosis of the disease., Methods: The clinical data of 32 DPB patients were collected, analyzed and summarized from March 1, 2013 to March 1, 2022 in the Second Xiangya Hospital of Central South University. The basic information, clinical manifestations, laboratory tests, pulmonary function, imaging tests, treatment, and regression of patients were analyzed., Results: A total of 32 patients were enrolled in the final analysis, with a male-to-female ratio at 1.67. The median age at symptom onset was 26.5 (11.0-69.0) years, and the median age of diagnosis was 47.5 (16.0-77.0) years. All patients presented with chronic cough and copious sputum production. A total of 26 patients had post activity shortness of breath and 14 patients had a positive result (blood cold agglutination test titer≥1꞉64). Pulmonary function examination was performed in 31 patients, 18 patients showed mixed pulmonary ventilation dysfunction, 12 patients showed obstructive pulmonary ventilation, and 1 patient had normal pulmonary ventilation function. A total of 31 patients had a bilateral, diffuse, small nodule pattern on chest CT. All patients were treated with macrolides. A total of 31 patients showed improvement, and 20 patients showed improvement in partial pressure of oxygen and blood oxygen saturation compared with before at discharge. A total of 12 patients were re-examined by chest CT after completing macrolides treatment, 6 cases showed less diffuse nodules, 5 cases showed no significant changes, and 1 case showed more diffuse nodules, which indicated the disease progression. Seven patients received pulmonary function tests after completing macrolides treatment, forced expiratory volume in one second (FEV
1 ) and FEV1 /forced vital capacitywere improved, but forced expiratory flow at 25% of vital capacity did not change significantly., Conclusions: The clinical manifestations of DPB are nonspecific. Early diagnosis and treatment are very important for the prognosis of patients.- Published
- 2023
- Full Text
- View/download PDF
14. Letter to the Editor: Huge Tricuspid Vegetation Caused by Haemophilus parainfluenzae .
- Author
-
Hu X, Liang W, An Z, and Hou J
- Subjects
- Humans, Haemophilus parainfluenzae, Haemophilus Infections diagnosis, Haemophilus Infections drug therapy
- Published
- 2022
- Full Text
- View/download PDF
15. Naturally acquired antibody against Haemophilus influenzae type a in pediatric saliva.
- Author
-
Cabrera A, Nix EB, Hickman R, Zlosnik JEA, Ulanova M, and Sadarangani M
- Subjects
- Antibodies, Bacterial, Child, Haemophilus influenzae, Humans, Immunoglobulin A, Infant, Saliva, Haemophilus Infections diagnosis
- Abstract
We developed a salivary assay for the detection of naturally acquired IgA antibody against Haemophilus influenzae type a (Hia) capsular polysaccharide in healthy Indigenous children from Northwestern Ontario, Canada. Hia-specific IgA antibody was detected in the saliva of 93% of Indigenous children aged 2-7 years., (Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved.)
- Published
- 2022
- Full Text
- View/download PDF
16. Haemophilus influenzae bacteremia: A 5-year (2016-2020) retrospective study analysing the clinical and laboratory features.
- Author
-
Tiewsoh JBA, Gupta P, Angrup A, and Ray P
- Subjects
- Anti-Bacterial Agents pharmacology, Anti-Bacterial Agents therapeutic use, Cefotaxime, Child, Haemophilus influenzae, Humans, Male, Microbial Sensitivity Tests, Retrospective Studies, Bacteremia diagnosis, Haemophilus Infections diagnosis, Haemophilus Infections epidemiology
- Abstract
Haemophilus influenzae (H. influenzae) causes invasive disease like bacteremia which is rarely diagnosed. We conducted this retrospective study of H. influenzae bacteremia diagnosed between January 2016 and December 2020. Nineteen patients were identified. Majority were children ≤5 years of age (84.2%), inpatients (89.5%), males (78.9%) and admitted in ICUs (26.6%). The most common underlying primary diagnosis was malignancy. These isolates were most susceptible to carbapenems (100%) followed by cefotaxime (83.3%) and ampicillin (82.4%). The overall mortality rate was 33.3%. Increased mortality resulted in those admitted in ICUs with H. influenzae bacteremia episode along with polymicrobial/co-isolates infection., (Copyright © 2022 Indian Association of Medical Microbiologists. Published by Elsevier B.V. All rights reserved.)
- Published
- 2022
- Full Text
- View/download PDF
17. Occurrence of Haemophilus influenzae type b (Hib) meningitis in a 2 month-old infant in the Hib vaccination era.
- Author
-
Cho Y, Tomari K, Nagamine T, and Fujiwara N
- Subjects
- Antigens, Bacterial, Humans, Incidence, Infant, Vaccination, Haemophilus Infections diagnosis, Haemophilus Infections epidemiology, Haemophilus Infections prevention & control, Haemophilus influenzae type b, Meningitis
- Abstract
Competing Interests: Declaration of competing interest The authors declare no conflicts of interest associated with this manuscript.
- Published
- 2022
- Full Text
- View/download PDF
18. Epidemiology and burden of Haemophilus influenzae disease in Thai children before implementation of the routine immunisation programme: A National Health Data Analysis.
- Author
-
Intusoma U, Thewamit R, Thamcharoenvipas T, and Khantee P
- Subjects
- Child, Data Analysis, Haemophilus influenzae, Humans, Immunization Programs, Incidence, Infant, Thailand epidemiology, Haemophilus Infections diagnosis, Haemophilus Infections epidemiology, Haemophilus Infections prevention & control, Haemophilus influenzae type b, Pneumonia epidemiology, Sepsis
- Abstract
Objectives: To conduct the first pre-Haemophilus influenzae (Hi) type b (Hib) immunisation programme-based epidemiological study using national health data., Methods: We analysed National Health Security Office data, which cover 72% of the Thai population. The study population included children aged <18 years admitted for Hi disease from 2015 to 2019. Hi disease diagnosis and death were based on the International Statistical Classification of Diseases and Related Health Problems (10th revision) hospital discharge summary codes. We estimated the hospital cost per admission using diagnosis-related grouping with a global budget., Results: A total of 1125 children aged <18 years were admitted for Hi disease. During the 5-year-study, the annual incidence of Hi disease varied from 1.5 to 1.9 per 100,000 children, with an overall case fatality rate (CFR) of 2%. Pneumonia was the most common clinical form, followed by meningitis and sepsis. The incidence, clinical forms and severity of Hi disease were age specific. Infant CFR was higher than that of other age groups. The incidence of Hi disease in children aged <5 years was 4.9 per 100,000 (CFR = 2.0%). Sepsis was the primary cause of infant death, whereas pneumonia was the cause of death in children aged >5 years. The hospital cost ranged from 25,000 to 30,000 THB per admission., Conclusions: This analysis provided epidemiological data of Hi in Thai children before the Hib routine immunisation programme. The incidence of Hi disease was lower than that previously speculated. Our results could facilitate an assessment of the impact of Hib immunisation programme in Thailand., (© 2022 John Wiley & Sons Ltd.)
- Published
- 2022
- Full Text
- View/download PDF
19. A Case Report: Ruptured Popliteal Aneurysm Caused by Haemophilus Influenzae Infection.
- Author
-
Bunde SG, Osigwe CC, and Hager E
- Subjects
- Aged, 80 and over, Aneurysm, Infected diagnostic imaging, Aneurysm, Infected surgery, Aneurysm, Ruptured diagnostic imaging, Aneurysm, Ruptured surgery, Anti-Bacterial Agents therapeutic use, Debridement, Female, Haemophilus Infections complications, Haemophilus Infections diagnosis, Haemophilus Infections surgery, Humans, Popliteal Artery diagnostic imaging, Popliteal Artery surgery, Suture Techniques, Treatment Outcome, Vascular Surgical Procedures, Aneurysm, Infected microbiology, Aneurysm, Ruptured microbiology, Haemophilus Infections microbiology, Haemophilus influenzae pathogenicity, Popliteal Artery microbiology
- Abstract
Mycotic aneurysms arise from infection of an arterial wall secondary to septic emboli from endocarditis. Although rare, most mycotic aneurysms involve the abdominal aorta, with Staphylococcus aureus and Salmonella spp being the most common causative organisms. We report a case of an 81-year-old woman with a ruptured mycotic popliteal aneurysm from Haemophilus influenzae infection., (Copyright © 2021 Elsevier Inc. All rights reserved.)
- Published
- 2022
- Full Text
- View/download PDF
20. Infective Uvulitis in a Child.
- Author
-
De Pieri C, Valentini E, Pusiol A, Passone E, Gamalero L, and Cogo PE
- Subjects
- Child, Child, Preschool, Female, Humans, Streptococcus pyogenes, Uvula, Epiglottitis diagnosis, Epiglottitis drug therapy, Haemophilus Infections diagnosis, Haemophilus Infections drug therapy, Pharyngitis, Stomatitis
- Abstract
Abstract: Infective uvulitis is a rare condition in children. In this report, we describe the case of a 4-year old-patient who presented a group A Streptococcus pharyngitis with uvulitis. No signs of epiglottitis were detected at nasal fibroscopy. She recovered rapidly with intravenous antibiotic therapy and 2 days of corticosteroid. Uvulitis is usually caused by group A Streptococcus or Haemophilus influentiae, but also other bacteria can be detected. Uvulitis can be isolated, or it can occur with epiglottitis and become an emergency., Competing Interests: Disclosure: The authors declare no conflict of interest., (Copyright © 2020 Wolters Kluwer Health, Inc. All rights reserved.)
- Published
- 2022
- Full Text
- View/download PDF
21. Rapid syndromic PCR testing in patients with respiratory tract infections reduces time to results and improves microbial yield.
- Author
-
Serigstad S, Markussen D, Grewal HMS, Ebbesen M, Kommedal Ø, Heggelund L, van Werkhoven CH, Faurholt-Jepsen D, Clark TW, Ritz C, Ulvestad E, Bjørneklett R, and Knoop ST
- Subjects
- Aged, Aged, 80 and over, Anti-Bacterial Agents therapeutic use, Clinical Decision-Making, Community-Acquired Infections drug therapy, Community-Acquired Infections microbiology, Community-Acquired Infections virology, Feasibility Studies, Female, Haemophilus Infections drug therapy, Haemophilus Infections microbiology, Humans, Influenza, Human drug therapy, Influenza, Human virology, Male, Middle Aged, Pneumococcal Infections drug therapy, Pneumococcal Infections microbiology, Pneumonia, Bacterial drug therapy, Pneumonia, Bacterial microbiology, Pneumonia, Viral drug therapy, Pneumonia, Viral virology, Predictive Value of Tests, Prospective Studies, Reproducibility of Results, Community-Acquired Infections diagnosis, Haemophilus Infections diagnosis, Haemophilus influenzae genetics, Influenza A virus genetics, Influenza, Human diagnosis, Multiplex Polymerase Chain Reaction, Pneumococcal Infections diagnosis, Pneumonia, Bacterial diagnosis, Pneumonia, Viral diagnosis, Streptococcus pneumoniae genetics
- Abstract
Lack of rapid and comprehensive microbiological diagnosis in patients with community acquired pneumonia (CAP) hampers appropriate antimicrobial therapy. This study evaluates the real-world performance of the BioFire FilmArray Pneumonia panel plus (FAP plus) and explores the feasibility of evaluation in a randomised controlled trial. Patients presenting to hospital with suspected CAP were recruited in a prospective feasibility study. An induced sputum or an endotracheal aspirate was obtained from all participants. The FAP plus turnaround time (TAT) and microbiological yield were compared with standard diagnostic methods (SDs). 96/104 (92%) enrolled patients had a respiratory tract infection (RTI); 72 CAP and 24 other RTIs. Median TAT was shorter for the FAP plus, compared with in-house PCR (2.6 vs 24.1 h, p < 0.001) and sputum cultures (2.6 vs 57.5 h, p < 0.001). The total microbiological yield by the FAP plus was higher compared to SDs (91% (162/179) vs 55% (99/179), p < 0.0001). Haemophilus influenzae, Streptococcus pneumoniae and influenza A virus were the most frequent pathogens. In conclusion, molecular panel testing in adults with CAP was associated with a significant reduction in time to actionable results and increased microbiological yield. The impact on antibiotic use and patient outcome should be assessed in randomised controlled trials., (© 2022. The Author(s).)
- Published
- 2022
- Full Text
- View/download PDF
22. Percutaneous Management of Septic Left Anterior Descending Coronary Occlusion Following Minimally Invasive Surgery for Mitral Valve Infective Endocarditis.
- Author
-
Leboutte PH, Demers P, Joubert P, and Marquis-Gravel G
- Subjects
- Adult, Coronary Angiography, Coronary Occlusion complications, Coronary Occlusion diagnosis, Coronary Vessels diagnostic imaging, Coronary Vessels surgery, Endocarditis diagnosis, Endocarditis microbiology, Female, Haemophilus isolation & purification, Haemophilus Infections diagnosis, Haemophilus Infections microbiology, Heart Valve Diseases surgery, Humans, ST Elevation Myocardial Infarction diagnosis, ST Elevation Myocardial Infarction etiology, Sepsis microbiology, Coronary Occlusion surgery, Endocarditis complications, Haemophilus Infections complications, Mitral Valve surgery, Percutaneous Coronary Intervention methods, ST Elevation Myocardial Infarction surgery, Sepsis complications
- Abstract
Coronary artery embolization is an unusual complication following infective endocarditis (IE) surgery. A 43-year-old woman developed an anterior ST-elevation myocardial infarction (STEMI) with acute left anterior descending artery occlusion due to septic emboli during the immediate postoperative period following minimally invasive mitral valve repair for IE. It was successfully treated with thromboaspiration and balloon angioplasty. Coronary septic emboli should be part of the differential diagnosis in patients presenting with STEMI during the early postoperative period for IE., (Copyright © 2021 Canadian Cardiovascular Society. Published by Elsevier Inc. All rights reserved.)
- Published
- 2022
- Full Text
- View/download PDF
23. Rheumatoid Arthritis Accompanying Diffuse Panbronchiolitis.
- Author
-
Isono T, Shibata S, Matsui Y, Hosoda C, Kawabata Y, Shimizu Y, and Takayanagi N
- Subjects
- Female, Humans, Lung, Middle Aged, Arthritis, Rheumatoid complications, Arthritis, Rheumatoid diagnosis, Bronchiolitis diagnosis, Bronchiolitis diagnostic imaging, Haemophilus Infections complications, Haemophilus Infections diagnosis
- Abstract
A 58-year-old woman with rheumatoid arthritis (RA) visited our hospital complaining of a persistent cough and sputum for the past year. She had a high cold hemagglutinin titer and chronic sinusitis. Chest computed tomography revealed bilateral diffuse centrilobular nodules, bronchiectasis, and bronchial wall thickening. A surgical lung biopsy was performed that confirmed diffuse panbronchiolitis (DPB) because of the lymphocytic and plasmacytic infiltrates in the respiratory bronchioles. Her condition improved after the administration of clarithromycin. Several cases of RA complicating DPB have previously been reported, but only in Japan. We need to consider DPB as a bronchiolitis types accompanying RA among Japanese patients.
- Published
- 2021
- Full Text
- View/download PDF
24. Haemophilus Influenzae Endogenous Endophthalmitis in an Immunocompetent Host.
- Author
-
Tabuenca Del Barrio L, Mozo Cuadrado M, Compains Silva E, Aliseda Pérez De Madrid D, and Heras Mulero H
- Subjects
- Adolescent, Anti-Bacterial Agents therapeutic use, Cataract Extraction, Cefotaxime therapeutic use, Ceftazidime therapeutic use, Ciprofloxacin therapeutic use, Drug Therapy, Combination, Endophthalmitis diagnosis, Endophthalmitis drug therapy, Endotamponade, Eye Infections, Bacterial diagnosis, Eye Infections, Bacterial drug therapy, Haemophilus Infections diagnosis, Haemophilus Infections drug therapy, Humans, Immunocompromised Host, Infusions, Intravenous, Intravitreal Injections, Male, Slit Lamp Microscopy, Visual Acuity physiology, Vitreous Body microbiology, Endophthalmitis microbiology, Eye Infections, Bacterial microbiology, Haemophilus Infections microbiology, Haemophilus influenzae isolation & purification
- Abstract
Purpose : To report a case of endogenous endophthalmitis caused by Haemophilus influenzae in an immunocompetent host. Case report : A 13-year-old male presented with pain, blurriness, and decreased Visual Acuity (VA) of Hand Motion in his right eye. Slit-lamp examination revealed hypopyon. Fundoscopy showed vitritis, vasculitis, and retinal infiltrates. Echography revealed vitreous condensations. Empirical treatment with intravitreal Ganciclovir, oral Valacyclovir, and Dexamethasone was initiated with no improvement. Vitreous culture revealed Haemophilus influenzae growth. Then, intravenous Ciprofloxacin and Cefotaxime and intravitreal Ceftazidime were administered with gradual improvement. Lensectomy with Pars Plana Vitrectomy and intraocular tamponade was performed. Nevertheless, the visual outcome was poor. Conclusion : Pediatric endogenous bacterial endophthalmitis is a rare but potentially devastating infection which is often misdiagnosed. Moreover, we want to highlight the importance of an adequate clinical suspicion in cases of H. influenzae to prevent the serious complications seen in this report.
- Published
- 2021
- Full Text
- View/download PDF
25. Spontaneous Abscess of the Posterior Nasal Septum: An Unusual Cause of Nasal Obstruction in Children.
- Author
-
Berlucchi M, Tomasoni M, Bosio R, and Rampinelli V
- Subjects
- Abscess complications, Abscess therapy, Child, Female, Haemophilus Infections complications, Haemophilus Infections therapy, Humans, Nasal Obstruction diagnosis, Nasal Obstruction therapy, Abscess diagnosis, Haemophilus Infections diagnosis, Haemophilus influenzae, Nasal Obstruction etiology, Nasal Septum
- Abstract
Introduction: Nasal septal abscess is a rare disorder in pediatric patients and is mostly diagnosed as a complication of trauma or secondary to dental or sinonasal infection., Case Presentation: A 10-year-old girl presented with acute nasal obstruction, otalgia, and headache. Medical history was negative; physical examination and anterior rhinoscopy did not reveal signs of acute rhinosinusitis or septal abscess., Management and Outcome: A thorough nasal endoscopy was performed to rule out a posterior nasal infection, revealing a bilateral posterior septal bulging, in the absence of purulent discharge. CT scan and contrast-enhanced MRI were performed, confirming the diagnosis of a spontaneous posterior septal abscess. Trans-nasal endoscopic-assisted drainage was conducted under general anesthesia. Haemophilus influenzae was detected on culture., Discussion: Although rare, nasal septal abscess in the pediatric age is typically anterior and secondary to local trauma or infection. Accurate medical history and anterior rhinoscopy are usually sufficient to make the diagnosis. In cases where the clinical presentation is consistent with a nasal septal abscess, with no history of recent local trauma or infection, or signs of anterior septal bulging, nasal endoscopy should be performed to rule out spontaneous posterior septal abscess. Delay in diagnosis and treatment could potentially cause the rapid onset of life-threatening complications, including intracranial abscess, meningitis, and cavernous sinus thrombosis.
- Published
- 2021
- Full Text
- View/download PDF
26. Haemophilus urethritis in males: A series of 30 cases.
- Author
-
Vives A, da Silva GVM, Alonso-Tarrés C, Suarez JB, Palmisano F, and Cosentino M
- Subjects
- Adult, Aged, Aged, 80 and over, Female, Haemophilus genetics, Haemophilus Infections diagnosis, Haemophilus Infections epidemiology, Humans, Male, Middle Aged, Multiplex Polymerase Chain Reaction, Retrospective Studies, Urethritis diagnosis, Urethritis epidemiology, Urethritis microbiology, Young Adult, Anti-Bacterial Agents therapeutic use, Haemophilus isolation & purification, Haemophilus Infections drug therapy, Urethra microbiology, Urethritis drug therapy
- Abstract
Introduction and Objectives: Pathogens such as Haemophilus spp. have been associated with non-gonococcal urethritis, but their role is unproven. To describe the clinical characteristics and therapeutic outcomes in male patients diagnosed with Haemophilus spp. urethritis., Methods: We carried out a retrospective study of all patients who presented to our hospital (in either the emergency department or the outpatient clinic) between July 2016 and April 2018 in whom Haemophilus spp. was isolated in the urethral samples. We enrolled 30 men with Haemophilus spp.-positive urethritis, including coinfections with Neisseria gonorrhoeae and Chlamydia trachomatis. Clinical, laboratory, demographic, and behavioral data were obtained by reviewing medical histories., Results: The mean age of the patients was 36.6 years (range 21-87). Seventeen patients (63%) reported being exclusively heterosexual. Three patients (10%) were HIV infected, all of them with an undetectable viral load. The most common clinical presentation was mucopurulent urethral discharge, in 13 patients (43%). The antibiotic treatment achieved a complete clinical resolution in 73%., Conclusions: Haemophilus urethritis affected men regardless of their sexual orientation or HIV status. Unprotected oral sex may play a role in its transmission. The limitations of the study preclude verification of the pathogenic role of Haemophilus spp. in acute urethritis, but clinical response after antibiotic treatment suggests that Haemophilus spp. can play such a role., (Copyright © 2020 Asociación Española de Andrología, Medicina Sexual y Reproductiva. Publicado por Elsevier España, S.L.U. All rights reserved.)
- Published
- 2021
- Full Text
- View/download PDF
27. Invasive Haemophilus influenzae type a infection and polyarthritis in a 72-year-old Japanese man: A case report.
- Author
-
Kenzaka T, Goda K, Kubota M, Takayanagi K, Kenri T, Shibayama K, and Akita H
- Subjects
- Aged, Australia, Brazil, Canada, Haemophilus influenzae genetics, Humans, Japan, Male, Arthritis, Haemophilus Infections diagnosis, Haemophilus Infections drug therapy
- Abstract
Haemophilus influenzae is a small, nonmotile, non-spore-forming bacterium classified into 6 serotypes (a to f) and non-typeable strains that lack a capsule. Although H. influenzae serotype a (Hia) is prevalent in Canada, the United States, Brazil, Australia, across the African continent, and several other locations, it has not been reported in Japan thus far. Our case was of a 72-year-old Japanese man who sought medical consultation after presenting with chills, fever, and polyarthritis. Cultures of blood and synovial fluid from the left knee revealed H. influenzae infection. Diagnostic imaging showed poor contrast regions in both kidneys, fluid retention around both knee joints, the left shoulder joint, and both elbow joints. Subsequently, the patient was diagnosed with invasive H. influenzae infection accompanied by polyarthritis and renal infarction. 16S ribosomal RNA gene sequencing revealed that the bacterial strain was Hia. The patient was treated with antimicrobial agents and arthroscopic curettage. We present a case of invasive Hia infection accompanied by polyarthritis and renal infarction. To the best of our knowledge, this is the first case of Hia infection in Japan. The case is very rare considering that the disease occurred in an elderly patient who developed polyarthritis., (Copyright © 2021 Japanese Society of Chemotherapy and The Japanese Association for Infectious Diseases. Published by Elsevier Ltd. All rights reserved.)
- Published
- 2021
- Full Text
- View/download PDF
28. Association of Bacteremia with Vaccination Status in Children Aged 2 to 36 Months.
- Author
-
Dunnick J, Taft M, Tisherman RT, Nowalk AJ, Hickey RW, and Wilson PM
- Subjects
- Bacteremia diagnosis, Bacteremia epidemiology, Bacteremia etiology, Child, Preschool, Emergency Service, Hospital, Female, Haemophilus Infections diagnosis, Haemophilus Infections epidemiology, Haemophilus Infections etiology, Humans, Incidence, Infant, Male, New England epidemiology, Pneumococcal Infections diagnosis, Pneumococcal Infections epidemiology, Retrospective Studies, Risk Factors, Treatment Outcome, Bacteremia prevention & control, Haemophilus Infections prevention & control, Haemophilus Vaccines, Haemophilus influenzae type b isolation & purification, Pneumococcal Infections prevention & control, Pneumococcal Vaccines, Vaccination Coverage statistics & numerical data
- Abstract
Objective: To determine the association between bacteremia and vaccination status in children aged 2-36 months presenting to a pediatric emergency department., Study Design: Retrospective cohort study of children aged 2-36 months with blood cultures obtained in the pediatric emergency department between January 2013 and December 2017. The exposure of interest was immunization status, defined as number of Haemophilus influenzae type B (Hib) and Streptococcus pneumoniae vaccinations, and the main outcome positive blood culture. Subjects with high-risk medical conditions were excluded., Results: Of 5534 encounters, 4742 met inclusion criteria. The incidence of bacteremia was 1.5%. The incidence of contaminated blood culture was 5.0%. The relative risk of bacteremia was 0.79 (95% CI 0.39-1.59) for unvaccinated and 1.20 (95% CI 0.52-2.75) for undervaccinated children relative to those who had received age-appropriate vaccines. Five children were found to have S pneumoniae bacteremia and 1 child had Hib bacteremia; all of these subjects had at least 3 sets of vaccinations. No vaccine preventable pathogens were isolated from blood cultures of unvaccinated children. We found no S pneumoniae or Hib in children 2-6 months of age who were not fully vaccinated due to age (95% CI 0-0.13%) and the contamination rate in this group was high compared with children 7-36 months (6.6% vs 3.7%)., Conclusions: Bacteremia in young children is an uncommon event. Contaminated blood cultures were more common than pathogens. Bacteremia from S pneumoniae or Hib is uncommon and, in this cohort, was independent of vaccine status., (Copyright © 2021 Elsevier Inc. All rights reserved.)
- Published
- 2021
- Full Text
- View/download PDF
29. Construction and Application of MALDI-TOF Mass Spectrometry for the Detection of Haemophilus parasuis.
- Author
-
Wang X, Xu F, Ning K, Shen L, Qi X, and Wang J
- Subjects
- Animals, Swine, Haemophilus Infections diagnosis, Haemophilus Infections microbiology, Haemophilus parasuis chemistry, Haemophilus parasuis classification, Haemophilus parasuis isolation & purification, Peptide Mapping methods, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization methods, Swine Diseases diagnosis, Swine Diseases microbiology
- Abstract
To construct a protein fingerprint database of Haemophilus parasuis ( H. parasuis ), thus improving its clinical diagnosis efficiency. A total of 15 H. parasuis standard strains were collected to establish a protein fingerprint database of H. parasuis using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS), and the effects of different culture media and culture time on the quality and identification results of the protein fingerprint were investigated. The results showed that tryptone soy agar (TSA) and tryptone soy broth (TSB) media and different incubation times had no significant effect on the characteristic peaks of the protein profiles. In addition, 18 clinical isolates were used to compare the identification results of the self-built protein fingerprint database, PCR detection, and basic database. Only one strain was identified in the original VITEK-MS system database, while the self-made protein fingerprint database of H. parasuis was 100% accurate for the detection of 18 clinical isolate strains. The protein fingerprint database of H. parasuis built by our laboratory is suitable for rapid clinical diagnosis of H. parasuis , due to its high accuracy, efficiency, and strong specificity., Competing Interests: The authors declare that they have no conflicts of interest., (Copyright © 2021 Xiaoxu Wang et al.)
- Published
- 2021
- Full Text
- View/download PDF
30. Pyelonephritis and bacteremia caused by Haemophilus parainfluenzae: case-report of an unusual pathogen.
- Author
-
Nevejan L, Goegebuer T, Mast P, and Lemmens A
- Subjects
- Haemophilus, Haemophilus parainfluenzae, Humans, Male, Bacteremia diagnosis, Haemophilus Infections diagnosis, Pyelonephritis diagnosis
- Abstract
We present a case of a young man with known urinary tract abnormalities who developed pyelonephritis and bacteremia caused by Haemophilus parainfluenzae. Since routine urine culture usually does not include enriched media for Haemophilus spp., the true incidence of urinary tract infections caused by H. parainfluenzae is currently unknown. Our case, however, demonstrates that H. parainfluenzae is a potential urinary pathogen, at least in patients with urinary tract anomalies. Clinical laboratories should consider expanding their culture efforts to detect unusual pathogens in patients with underlying risk factors.
- Published
- 2021
- Full Text
- View/download PDF
31. Development of a Luminex microbead-based serotyping assay for Glaesserella parasuis.
- Author
-
Yee S, Meers J, Parke CR, Barnes TS, Blackall PJ, and Turni C
- Subjects
- Animals, Australia, Swine, Haemophilus Infections diagnosis, Haemophilus Infections microbiology, Haemophilus parasuis isolation & purification, Serogroup, Serotyping methods, Swine Diseases diagnosis, Swine Diseases microbiology
- Abstract
Glaesserella parasuis consists of 15 serovars with some of them highly virulent and some of them avirulent. As killed vaccines do not provide crossprotection across serovars, serotyping is of importance. Serotyping, previously done by gel diffusion, is now done by multiplex PCR followed by electrophoresis. Accurately differentiating 15 serovars by electrophoresis is problematic. To overcome this problem, a Luminex microbead-based multiplex assay was used to differentiate the serovars. The assay consisted of a multiplex PCR assay followed by hybridisation to microbeads which were then analysed on a Luminex machine. The newly developed assay was compared to the multiplex serotyping PCR and the gel diffusion/indirect haemagglutination assay (GD/IHA). The microbead-based assay worked very well for the 15 reference strains but when used on the 74 Australian field strains displayed some problems. The main problems were with the eight out of nine serovar 4 field isolates and the five serovar 7 and three serovar 14 field isolates. While the microbead-based assay could differentiate between the serovar 5 and 12 reference strains, which the serovar multiplex PCR could not, all four field isolates identified by GD/IHA as serovar 12 were identified as serovar 5 by the microbead-based assay. Serovar 4 has been noted to have a high diversity especially among strains from different countries. Our work clearly shows that the diversity of strains at both the national and the international level has to be taken into account when developing diagnostic assays., (Copyright © 2021 Elsevier B.V. All rights reserved.)
- Published
- 2021
- Full Text
- View/download PDF
32. Early detection of drug-resistant Streptococcus pneumoniae and Haemophilus influenzae by quantitative flow cytometry.
- Author
-
Sawada T, Katayama M, Takatani S, and Ohiro Y
- Subjects
- Anti-Bacterial Agents therapeutic use, Colony Count, Microbial, Feasibility Studies, Haemophilus Infections diagnosis, Haemophilus Infections drug therapy, Haemophilus Infections microbiology, Haemophilus influenzae drug effects, Humans, Microbial Sensitivity Tests methods, Pneumococcal Infections diagnosis, Pneumococcal Infections drug therapy, Pneumococcal Infections microbiology, Sensitivity and Specificity, Streptococcus pneumoniae drug effects, Anti-Bacterial Agents pharmacology, Drug Resistance, Bacterial, Flow Cytometry methods, Haemophilus influenzae isolation & purification, Streptococcus pneumoniae isolation & purification
- Abstract
Early detection of drug resistance contributes to combating drug-resistant bacteria and improving patient outcomes. Microbial testing in the laboratory is essential for treating infectious diseases because it can provide critical information related to identifying pathogenic bacteria and their resistance profiles. Despite these clinical requirements, conventional phenotypic testing is time-consuming. Additionally, recent rapid drug resistance tests are not compatible with fastidious bacteria such as Streptococcus and Haemophilus species. In this study, we validated the feasibility of direct bacteria counting using highly sensitive quantitative flow cytometry. Furthermore, by combining flow cytometry and a nucleic acid intercalator, we constructed a highly sensitive method for counting viable fastidious bacteria. These are inherently difficult to measure due to interfering substances from nutrients contained in the medium. Based on the conventional broth microdilution method, our method acquired a few microliter samples in a time series from the same microplate well to exclude the growth curve inconsistency between the samples. Fluorescent staining and flow cytometry measurements were completed within 10 min. Therefore, this approach enabled us to determine antimicrobial resistance for these bacteria within a few hours. Highly sensitive quantitative flow cytometry presents a novel avenue for conducting rapid antimicrobial susceptibility tests.
- Published
- 2021
- Full Text
- View/download PDF
33. Concurrent infection of Avibacterium paragallinarum and fowl adenovirus in layer chickens.
- Author
-
Mei C, Xian H, Blackall PJ, Hu W, Zhang X, and Wang H
- Subjects
- Adenoviridae classification, Adenoviridae genetics, Animals, Chickens, China, Haemophilus paragallinarum genetics, Phylogeny, Adenoviridae Infections complications, Adenoviridae Infections diagnosis, Adenoviridae Infections mortality, Adenoviridae Infections veterinary, Coinfection mortality, Coinfection pathology, Haemophilus Infections complications, Haemophilus Infections diagnosis, Haemophilus Infections mortality, Haemophilus Infections veterinary, Poultry Diseases microbiology, Poultry Diseases mortality, Poultry Diseases pathology, Poultry Diseases virology
- Abstract
The diagnosis of a concurrent infection of Avibacterium paragallinarum and fowl adenovirus (FAdV) in an infectious coryza-like outbreak in the outskirt of Beijing is reported. The primary signs of the infection were acute respiratory signs, a drop in egg production, and the presence of hydropericardium-hepatitis syndrome-like gross lesions. Laboratory examination confirmed the presence of A. paragallinarum by bacterial isolation and a species-specific PCR test. In addition, conventional serotyping identified the isolates as Page serovar A. Fowl adenovirus was isolated from chicken liver specimen and identified by hexon gene amplification. In addition, histopathologic analysis and transmission electron microscopy examination further confirmed the presence of the virus. Both hexon gene sequencing and phylogenetic analysis defined the viral isolate as FAdV-4. The pathogenic role of A. paragallinarum and FAdV was evaluated by experimental infection of specific-pathogen-free chickens. The challenge trial showed that combined A. paragallinarum and FAdV infection resulted in more severe clinical signs than that by FAdV infection alone. The concurrent infection caused 50% mortality compared with 40% mortality by FAdV infection alone and zero mortality by A. paragallinarum infection alone. To our knowledge, this is the first report of A. paragallinarum coinfection with FAdV. The case implies that concurrent infections with these 2 agents do occur and more attention should be given to the potential of multiple agents during disease diagnosis and treatment., (Copyright © 2020. Published by Elsevier Inc.)
- Published
- 2020
- Full Text
- View/download PDF
34. Development of a recombinase polymerase amplification assay for rapid detection of Haemophilus parasuis in tissue samples.
- Author
-
Han Q, Wang J, Li R, Han Q, Yuan W, and Wang J
- Subjects
- Animals, Haemophilus Infections diagnosis, Haemophilus Infections virology, Haemophilus parasuis enzymology, Nucleic Acid Amplification Techniques methods, Recombinases analysis, Sus scrofa, Swine, Swine Diseases virology, Haemophilus Infections veterinary, Haemophilus parasuis isolation & purification, Nucleic Acid Amplification Techniques veterinary, Swine Diseases diagnosis
- Abstract
Haemophilus parasuis is the etiological agent of Glässer's disease in swine, which associates with severe economic losses in the swine industry worldwide. A real-time recombinase polymerase amplification assay (real-time RPA) was developed for direct and rapid detection of H. parasuis basing on the translation-initiation factor IF2 (infB) gene. The assay was performed successfully at 39°C for 20 min in Genie III, which is portable and chargeable by battery. The developed assay was highly specific for H. parasuis, and the limit of detection of the assay was 6.0 × 10
3 fg of H. parasuis genomic DNA, which was the same as that of a real-time PCR developed previously. The assay was further evaluated on 68 pig tissue samples, and 18 (26.5%), 20 (29.4%), and 8 (11.8%) samples were positive for H. parasuis by the real-time RPA, real-time PCR and bacterial isolation, respectively. With the bacteria isolation as the reference method, the real-time RPA showed a diagnostic specificity of 83.33% and a diagnostic sensitivity of 100%. The above data demonstrated the well-potentiality and usefulness of the developed real-time RPA assay in reliable diagnosis of swine Glässer's disease, especially in resource limited settings., (© 2020 The Authors. Veterinary Medicine and Science published by John Wiley & Sons Ltd.)- Published
- 2020
- Full Text
- View/download PDF
35. Management of preseptal and orbital cellulitis for the primary care physician.
- Author
-
Gordon AA and Phelps PO
- Subjects
- Abscess diagnosis, Aspergillosis diagnosis, Aspergillosis immunology, Aspergillosis therapy, Cellulitis diagnosis, Cellulitis therapy, Haemophilus Infections diagnosis, Haemophilus Infections therapy, Humans, Immunocompromised Host immunology, Mucormycosis diagnosis, Mucormycosis immunology, Mucormycosis therapy, Orbital Cellulitis diagnosis, Periosteum, Physicians, Primary Care, Primary Health Care, Severity of Illness Index, Staphylococcal Infections diagnosis, Streptococcal Infections diagnosis, Tomography, X-Ray Computed, Abscess therapy, Anti-Bacterial Agents therapeutic use, Debridement, Drainage, Orbital Cellulitis therapy, Staphylococcal Infections therapy, Streptococcal Infections therapy
- Abstract
Preseptal cellulitis and postseptal (orbital) cellulitis represent a spectrum of orbital infections which a primary care provider and ophthalmologist may see during practice. Most often these conditions occur through spread from the sinuses, though there are a variety of other inciting factors. These conditions can both present with erythema and edema, but a complete and thorough exam can help a practitioner differentiate the two. Patients should be treated with targeting the most common pathogens and followed very closely by their providers. This article will discuss the anatomy, pathogenesis, risk factors, diagnosis, management and prognosis of preseptal and orbital cellulitis., (Copyright © 2020. Published by Elsevier Inc.)
- Published
- 2020
- Full Text
- View/download PDF
36. Discriminative Potential of the Vitek MS In Vitro Diagnostic Device Regarding Haemophilus influenzae and Haemophilus haemolyticus.
- Author
-
Nürnberg S, Claus H, Krone M, Vogel U, and Lâm TT
- Subjects
- Haemophilus, Humans, Reagent Kits, Diagnostic, Haemophilus Infections diagnosis, Haemophilus influenzae
- Published
- 2020
- Full Text
- View/download PDF
37. Invasive non-typeable Haemophilus influenzae infection due to endometritis associated with adenomyosis.
- Author
-
Nishimura Y, Hagiya H, Kawano K, Yokota Y, Oka K, Iio K, Hasegawa K, Obika M, Haruma T, Ono S, Masuyama H, and Otsuka F
- Subjects
- Adenomyosis microbiology, Ampicillin therapeutic use, Bacteremia diagnosis, Bacteremia microbiology, Bacterial Typing Techniques, Blood Culture, Drug Resistance, Multiple, Bacterial, Endometritis microbiology, Female, Haemophilus Infections blood, Haemophilus Infections complications, Haemophilus influenzae classification, Humans, Japan, Middle Aged, Reproductive Tract Infections diagnosis, Reproductive Tract Infections microbiology, Adenomyosis complications, Bacteremia etiology, Endometritis complications, Haemophilus Infections diagnosis, Haemophilus influenzae isolation & purification, Reproductive Tract Infections complications
- Abstract
Background: The widespread administration of the Haemophilus influenzae type b vaccine has led to the predominance of non-typable H. influenzae (NTHi). However, the occurrence of invasive NTHi infection based on gynecologic diseases is still rare., Case Presentation: A 51-year-old Japanese woman with a history of adenomyoma presented with fever. Blood cultures and a vaginal discharge culture were positive with NTHi. With the high uptake in the uterus with
67 Ga scintigraphy, she was diagnosed with invasive NTHi infection. In addition to antibiotic administrations, a total hysterectomy was performed. The pathological analysis found microabscess formations in adenomyosis., Conclusions: Although NTHi bacteremia consequent to a microabscess in adenomyosis is rare, this case emphasizes the need to consider the uterus as a potential source of infection in patients with underlying gynecological diseases, including an invasive NTHi infection with no known primary focus.- Published
- 2020
- Full Text
- View/download PDF
38. Hemophilus influenzae and Parainfluenza Virus Pneumonia in a Patient with AIDS.
- Author
-
Nagarakanti S and Bishburg E
- Subjects
- Anti-Bacterial Agents therapeutic use, Ceftriaxone therapeutic use, Coinfection, Haemophilus Infections drug therapy, Haemophilus influenzae isolation & purification, Humans, Male, Middle Aged, Respiration, Artificial, Respiratory Distress Syndrome etiology, Respiratory Distress Syndrome therapy, Acquired Immunodeficiency Syndrome, Antiviral Agents therapeutic use, Haemophilus Infections diagnosis, Paramyxoviridae Infections diagnosis, Paramyxoviridae Infections drug therapy, Pneumonia, Viral diagnosis, Pneumonia, Viral drug therapy, Ribavirin therapeutic use
- Abstract
BACKGROUND Parainfluenza viruses (PIV) are known to cause mild respiratory tract infections in immunocompetent patients but can cause severe infections in immune-compromised patients such as transplant recipients and children with HIV. PIV infection in HIV-infected adults has rarely been reported. We report a case of PIV pneumonia in an adult with AIDS who was successfully treated with oral ribavirin. CASE REPORT A 64-year-old man with history of acquired immune deficiency syndrome (AIDS) was admitted to the hospital with shortness of breath that began 3 days before. He was in respiratory distress and required mechanical ventilation on arrival. A bronchoalveolar lavage (BAL) culture was positive for Hemophilus influenzae and a respiratory viral panel was positive for Parainfluenza virus. The patient was initially started on Cefepime and Trimethoprim- Sulfamethoxazole and later changed to Ceftriaxone based on culture results. As the patient's condition did not improve after 48 h, oral ribavirin was added to treat PIV. The patient subsequently improved and was extubated after 72 h. CONCLUSIONS Oral ribavirin can have a beneficial effect in AIDS patients who have PIV-associated pneumonia. Further investigation of the benefit of oral ribavirin in similar cases is warranted.
- Published
- 2020
- Full Text
- View/download PDF
39. Dried blood spots for Streptococcus pneumoniae and Haemophilus influenzae detection and serotyping among children < 5 years old in rural Mozambique.
- Author
-
Pimenta FC, Moiane B, Lessa FC, Venero AL, Moura I, Larson S, Massora S, Chaúque A, Tembe N, Mucavele H, Verani JR, Whitney CG, Sigaúque B, and Carvalho MGS
- Subjects
- Aged, Carrier State, Child, Child, Preschool, Haemophilus influenzae genetics, Humans, Infant, Mozambique epidemiology, Nasopharynx, Serotyping, Streptococcus pneumoniae genetics, Haemophilus Infections diagnosis, Haemophilus Infections epidemiology, Pneumococcal Infections diagnosis
- Abstract
Background: Dried blood spots (DBS) have been proposed as potentially tool for detecting invasive bacterial diseases., Methods: We evaluated the use of DBS for S. pneumoniae and H. influenzae detection among children in Mozambique. Blood for DBS and nasopharyngeal (NP) swabs were collected from children with pneumonia and healthy aged < 5 years. Bacterial detection and serotyping were performed by quantitative PCR (qPCR) (NP and DBS; lytA gene for pneumococcus and hpd for H. influenzae) and culture (NP). Combined detection rates were compared between children with pneumonia and healthy., Results: Of 325 children enrolled, 205 had pneumonia and 120 were healthy. Pneumococci were detected in DBS from 20.5 and 64.2% of children with pneumonia and healthy, respectively; NP specimens were positive for pneumococcus in 80.0 and 80.8%, respectively. H. influenzae was detected in DBS from 22.9% of children with pneumonia and 59.2% of healthy; 81.4 and 81.5% of NP specimens were positive for H. influenzae, respectively., Conclusion: DBS detected pneumococcal and H. influenzae DNA in children with pneumonia and healthy. Healthy children were often DBS positive for both bacteria, suggesting that qPCR of DBS specimens does not differentiate disease from colonization and is therefore not a useful diagnostic tool for children.
- Published
- 2020
- Full Text
- View/download PDF
40. Three cases of diffuse panbronchiolitis in children with a past history of difficult-to-treat bronchial asthma: A case report from a single medical facility.
- Author
-
Fujita S, Suzuki R, Sagara N, Aota A, Akashi K, and Katsunuma T
- Subjects
- Adolescent, Asthma diagnosis, Asthma drug therapy, Child, Female, Humans, Lung diagnostic imaging, Lung pathology, Macrolides therapeutic use, Male, Treatment Outcome, Bronchiolitis diagnosis, Bronchiolitis drug therapy, Haemophilus Infections diagnosis, Haemophilus Infections drug therapy
- Published
- 2020
- Full Text
- View/download PDF
41. Cricoid Chondronecrosis: Case Report and Review of Literature.
- Author
-
Mims MM, Leclerc AA, and Smith LJ
- Subjects
- Adult, Alcoholism complications, Anti-Bacterial Agents therapeutic use, Cartilage Diseases complications, Cartilage Diseases pathology, Cartilage Diseases therapy, Cricoid Cartilage pathology, Depression complications, Dyspnea etiology, Haemophilus Infections complications, Haemophilus Infections therapy, Humans, Intubation, Intratracheal, Laryngoscopy, Laryngostenosis etiology, Male, Necrosis, Otorhinolaryngologic Surgical Procedures, Respiratory Sounds etiology, Streptococcal Infections complications, Streptococcal Infections therapy, Vocal Cord Paralysis diagnosis, Vocal Cord Paralysis etiology, Cartilage Diseases diagnosis, Cricoid Cartilage diagnostic imaging, Haemophilus Infections diagnosis, Laryngostenosis diagnosis, Streptococcal Infections diagnosis, Tracheostomy
- Abstract
Objective: Cricoid chondronecrosis is a rare entity that has significant consequences for patients. Reports of its occurrence are scattered in the literature and currently there is no comprehensive review to help guide providers., Methods: A case report from our institution is presented. A review of available literature is then provided with assessment of risk factors, signs and symptoms at presentation, laryngeal exam findings, radiologic findings, and surgical techniques., Results: Twenty-four cases of cricoid chondronecrosis were reviewed from the literature. Patient age ranged from 8 months to 76 years. A history of endotracheal intubation for various reasons was present in all but two cases, and duration of intubation ranged from 6 hours to 28 days. Patients presented with airway compromise in all but two cases-one asymptomatic patient with prior tracheostomy and another with dysphagia after radiation. Dysphonia (n = 6) and dysphagia (n = 3) were less commonly present. Subglottic stenosis (n = 19) was the most common exam finding followed by vocal fold impairment (n = 11). When CT scan findings were reported, fragmentation of the cartilage and/or hypodensity of the central lamina were described in all but one case. Interventions ranged from observation on antibiotics and steroids to surgical therapies including tracheostomy, dilation, and posterior cricoid split, with or without stent placement., Conclusions: Cricoid chondronecrosis is a serious, rare entity that can occur even after a short period of endotracheal intubation. Providers must have a high level of suspicion in patients that present with upper airway dyspnea with a history of prior intubation., Level of Evidence: Level 4.
- Published
- 2020
- Full Text
- View/download PDF
42. Detection of Cell-Dissociated Non-Typeable Haemophilus influenzae in the Airways of Patients with Chronic Obstructive Pulmonary Disease.
- Author
-
Thulborn SJ, Ceroni A, Haldar K, Mistry V, Cane JL, Brightling CE, Barer MR, and Bafadhel M
- Subjects
- Haemophilus influenzae, Humans, Severity of Illness Index, Sputum, Haemophilus Infections diagnosis, Pulmonary Disease, Chronic Obstructive diagnosis
- Abstract
Background: Non-typeable Haemophilus influenzae (NTHi) is the most commonly found pathogen in the lower respiratory airways of patients with COPD. NTHi is predominantly regarded as an intracellular pathogen; however, like most pathogens, it can exist and co-exist in two broad forms: cell-associated (intracellularly or adhered to cells) or cell-dissociated (biofilm dispersed or planktonic). We sought to investigate if cell-dissociated NTHi can be detected from the sputum of COPD patients and assess this relationship to disease severity and airway inflammation., Methods: DNA was extracted from the sputum plug and cell-free supernatant to quantify absolute (cell-associated and cell-dissociated NTHi) and cell-dissociated NTHi, respectively, from 87 COPD subjects attending an observational longitudinal COPD exacerbation study. NTHi was quantified using TaqMan hydrolysis probes, targeting the OMP P6 gene using qPCR., Results: At stable state cell-dissociated NTHi was detected 56% of subjects with a median (IQR) of 9.95x10
2 gene copies (1.26x102 to 1.90x104 ). Cell-dissociated NTHi correlated with absolute NTHi levels (r=0.34, p<0.01) but not airway inflammation or spirometry at stable state. At exacerbation, cell-dissociated NTHi correlated with lung function (FEV1 r=0.629, p=0.005; FEV1 %predicted r=0.564, p=0.015; FVC r=0.476 p=0.046) and sputum neutrophilic inflammation (% neutrophils r=0.688, p=0.002; total neutrophils r=0.518, p=0.028)., Conclusion: In patients with COPD, NTHi can exist in both cell-associated and cell-dissociated forms. Cell-dissociated NTHi is associated with neutrophilic airway inflammation during exacerbations of COPD and may be a driving factor in worsening lung function during these episodes., Competing Interests: CEB was funded by a Wellcome Trust Senior Fellowship. MB was funded by a National Institute for Health Research (NIHR) Fellowship. MB has received travel support from AstraZeneca, Boehringer Ingelheim and GlaxoSmithKline and consultancy honoraria outside the submitted work from AstraZeneca, Boehringer Ingelheim, Chiesi, GlaxoSmithKline and Pfizer. CEB has received grant support and consultancy honoraria outside the submitted work from AstraZeneca, Boehringer Ingelheim, Chiesi, GlaxoSmithKline, Medimmune, Novartis and Roche. IDP has received grant support and consultancy honoraria outside the submitted work from Aerocrine, AstraZeneca, Boehringer Ingelheim, GlaxoSmithKline and Novartis. The authors report no conflicts of interest in this work., (© 2020 Thulborn et al.)- Published
- 2020
- Full Text
- View/download PDF
43. A severe case with co-infection of SARS-CoV-2 and common respiratory pathogens.
- Author
-
Ou X, Zhou L, Huang H, Lin Y, Pan X, and Chen D
- Subjects
- Aged, COVID-19, Coinfection virology, Coronavirus Infections virology, Female, Haemophilus Infections microbiology, High-Throughput Nucleotide Sequencing, Humans, Moraxellaceae Infections microbiology, Pandemics, Patient Isolation methods, Patient Transfer methods, Pneumonia, Viral virology, SARS-CoV-2, Sputum virology, Betacoronavirus genetics, Coinfection diagnosis, Coronavirus Infections diagnosis, Haemophilus Infections diagnosis, Haemophilus parainfluenzae genetics, Moraxella catarrhalis genetics, Moraxellaceae Infections diagnosis, Pneumonia, Viral diagnosis
- Abstract
Competing Interests: Declaration of competing interest All authors declare no financial, potential personal or commercial conflict of interest.
- Published
- 2020
- Full Text
- View/download PDF
44. Coexistence of diffuse panbronchiolitis and sarcoidosis revealed during splenectomy: a case report.
- Author
-
Akaba T, Takeyama K, Kondo M, Kobayashi F, Okabayashi A, Sawada T, and Tagaya E
- Subjects
- Bronchiolitis drug therapy, Bronchoalveolar Lavage Fluid virology, CD4-Positive T-Lymphocytes metabolism, CD8-Positive T-Lymphocytes metabolism, Cough etiology, Female, Granuloma diagnostic imaging, Granuloma pathology, Haemophilus Infections drug therapy, Haemophilus influenzae isolation & purification, Humans, Lung diagnostic imaging, Macrolides therapeutic use, Sarcoidosis surgery, Splenectomy, Tomography, X-Ray Computed, Young Adult, Bronchiolitis complications, Bronchiolitis diagnosis, Haemophilus Infections complications, Haemophilus Infections diagnosis, Lung pathology, Sarcoidosis complications, Sarcoidosis diagnosis
- Abstract
Background: Sarcoidosis is a systemic granulomatous disease caused by CD4+ cell-dominant inflammation. Meanwhile, diffuse panbronchiolitis is a chronic inflammatory respiratory disease predominantly caused by CD8+ lymphocytes and neutrophils. Herein, we report a rare case of sarcoidosis in which the clinical presentation had become evident as diffuse panbronchiolitis after splenectomy for sarcoidosis., Case Presentation: A 23-year-old Japanese woman was referred to our hospital due to splenomegaly of unknown etiology. Upon admission, chest computed tomography scan revealed centrilobular and randomly distributed small nodules in both lungs. Bronchoalveolar lavage revealed a high proportion of lymphocytes and a decreased CD4/CD8 ratio. However, the biopsy specimens obtained from both the liver and lungs revealed noncaseating epithelioid granulomas, which confirmed the diagnosis of sarcoidosis. The patient underwent splenectomy due to progressive cytopenia and high risk of splenic rupture. After the surgery, the condition of the patient was consistently good for 3 months. Then, she gradually developed productive cough and dyspnea. Both sinus and chest computed tomography scan revealed chronic paranasal sinusitis and deterioration of centrilobular nodules in both lung fields, respectively. The second bronchoalveolar lavage revealed a high proportion of neutrophils, and the bronchoalveolar lavage fluid tested positive for Hemophilus influenzae. The titer of cold agglutinin was elevated, thereby confirming the diagnosis of diffuse panbronchiolitis. On the basis of the clinical and radiological findings, the condition of the patient improved with low-dose macrolide therapy for 3 months., Conclusions: The coexistence of sarcoidosis and diffuse panbronchiolitis has not been previously reported, and the hidden profiles of diffuse panbronchiolitis may have been revealed by splenectomy.
- Published
- 2020
- Full Text
- View/download PDF
45. Small Airway Disease: A Step Closer to Etiology-Based Classification of Bronchiolitis.
- Author
-
Urisman A and Jones KD
- Subjects
- Bronchi pathology, Bronchiolitis classification, Bronchiolitis diagnosis, Bronchiolitis pathology, Bronchiolitis Obliterans diagnosis, Bronchiolitis Obliterans etiology, Bronchiolitis Obliterans pathology, Diagnosis, Differential, Haemophilus Infections diagnosis, Haemophilus Infections etiology, Haemophilus Infections pathology, Humans, Lung pathology, Bronchiolitis etiology
- Abstract
Three major histologic patterns of bronchiolitis: obliterative bronchiolitis, follicular bronchiolitis, and diffuse panbronchiolitis, are reviewed in detail. These distinct patterns of primary bronchiolar injury provide a useful starting point for formulating a differential diagnosis and considering possible causes. In support of the aim toward a cause-based classification system of small airway disease, a simple diagnostic algorithm is provided for further subclassification of the above 3 bronchiolitis patterns according to the major associated etiologic subgroups., Competing Interests: Disclosure The authors have nothing to disclose., (Copyright © 2019 Elsevier Inc. All rights reserved.)
- Published
- 2020
- Full Text
- View/download PDF
46. Pediatric Bacterial Pericarditis.
- Author
-
Donovan M, Smith N, Holton R, and Shapiro C
- Subjects
- Anti-Bacterial Agents therapeutic use, Child, Preschool, Echocardiography, Haemophilus Infections therapy, Humans, Male, Pericardiocentesis methods, Pericarditis microbiology, Pericarditis therapy, Tomography, X-Ray Computed, Haemophilus Infections diagnosis, Haemophilus influenzae isolation & purification, Pericarditis diagnosis
- Published
- 2020
- Full Text
- View/download PDF
47. Non-typeable Haemophilus influenzae meningitis in a 3-year-old child.
- Author
-
Corkery-Lavender T, Watson O, and Goktogan S
- Subjects
- Child, Preschool, Haemophilus influenzae, Humans, Infant, Haemophilus Infections diagnosis, Haemophilus Vaccines, Meningitis
- Published
- 2020
- Full Text
- View/download PDF
48. Lessons From the Past and Present.
- Author
-
Atamian EK and Steele RW
- Subjects
- Diagnosis, Differential, Fever etiology, Humans, Infant, Male, Vomiting etiology, Haemophilus Infections complications, Haemophilus Infections diagnosis, Haemophilus influenzae isolation & purification
- Published
- 2020
- Full Text
- View/download PDF
49. Primary ciliary dyskinesia caused by a large homozygous deletion including exons 1-4 of DRC1 in Japanese patients with recurrent sinopulmonary infection.
- Author
-
Keicho N, Hijikata M, Morimoto K, Homma S, Taguchi Y, Azuma A, and Kudoh S
- Subjects
- Adult, Cilia ultrastructure, Ciliary Motility Disorders genetics, Ciliary Motility Disorders microbiology, Diagnosis, Differential, Exons, Female, Homozygote, Humans, Male, Middle Aged, Multiplex Polymerase Chain Reaction methods, Bronchiolitis diagnosis, Ciliary Motility Disorders diagnosis, Gene Deletion, Genetic Testing methods, Haemophilus Infections diagnosis, Microtubule-Associated Proteins genetics
- Abstract
Background: Diffuse panbronchiolitis (DPB) is a sinopulmonary disease mainly affecting Asian populations. Primary ciliary dyskinesia (PCD) is a genetically heterogeneous disorder impairing ciliary structure and function. These two disorders are not easily distinguished by clinical signs and symptoms., Methods: In 105 Japanese patients with recurrent sinopulmonary infection, initially diagnosed with DPB, and 37 patients with recurrent airway infection diagnosed in adulthood, the deletion allele of DRC1 or CCDC164, recently recognized as a pathogenic PCD gene variant, was searched using a multiplexed PCR-based method, and the deletion breakpoints and other variants around the gene were determined by Sanger sequencing and targeted resequencing., Results: A large homozygous deletion in DRC1 was identified in three of the 142 patients. Furthermore, heterozygous carriers of the deletion with the same breakpoint were found with the allele frequency of 0.002 in the healthy Japanese population, indicating that this loss-of-function variant may be acting as a common mutation causing PCD in Japanese., Conclusion: PCD caused by the DRC1 defect is not readily identified by either high-speed video-microscopy or ciliary ultrastructure analysis, posing significant difficulties in reaching a correct diagnosis without the aid of genetic tests. Careful investigation of the causes of sinopulmonary diseases is warranted in Asian populations., (© 2019 The Authors. Molecular Genetics & Genomic Medicine published by Wiley Periodicals, Inc.)
- Published
- 2020
- Full Text
- View/download PDF
50. Development and application of an antibody detection ELISA for Haemophilus parasuis based on a monomeric autotransporter passenger domain.
- Author
-
Liu Y, Du Y, Song Y, Tian Y, Qi Y, Zhang Q, He Q, Wang X, Chen H, Cai X, and Xu X
- Subjects
- Animals, Bacterial Proteins genetics, Bacterial Proteins immunology, Bacterial Proteins metabolism, Bacterial Vaccines immunology, Enzyme-Linked Immunosorbent Assay methods, Gene Expression Regulation, Bacterial, Haemophilus Infections diagnosis, Haemophilus Infections microbiology, Haemophilus Infections prevention & control, Haemophilus parasuis immunology, Swine, Swine Diseases diagnosis, Swine Diseases prevention & control, Enzyme-Linked Immunosorbent Assay veterinary, Haemophilus Infections veterinary, Haemophilus parasuis isolation & purification, Swine Diseases microbiology, Type V Secretion Systems immunology
- Abstract
Background: Haemophilus parasuis is a commensal pathogen in the swine upper respiratory tract and causes Glässer's disease. Surveillance, screening for infection, and vaccination response of H. parasuis is hindered by the lack of a rapid antibody detection method., Results: In the present study, a monomeric autotransporter was identified as a novel antigen for developing an indirect ELISA. The autotransporter passenger domain (Apd) was expressed, purified, and demonstrated to be specific in ELISA and western blotting. Mouse antiserum of recombinant Apd (rApd) recognized native Apd in the 15 serotype reference strains and five non-typeable isolate stains, but showed no reaction with seven other bacterial pathogens. The rApd ELISA was optimized and validated using 67 serum samples with known background, including 27 positive sera from experimentally infected and vaccinated pigs along with 40 negative sera that had been screened with H. parasuis whole cell ELISA from clinically healthy herds. The rApd ELISA provided positive and negative percent agreements of 96.4 and 94.9%, respectively, and an AUC value of 0.961, indicating that the assay produced accurate results., Conclusion: Apd was a universal antigen component among 15 serotype and non-typeable strains of H. parasuis and was also specific to this pathogen. The rApd ELISA could detect antibodies elicited by H. parasuis infection and vaccination, thereby exhibiting the potential to be applied for Glässer's disease diagnosis, H. parasuis vaccination evaluation, and large-scale serological surveillance.
- Published
- 2019
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.