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Radiation-induced pulmonary fibrosis (RIPF) is a severe late-stage complication of radiotherapy (RT) to the chest area, typically used in lung cancer treatment. This condition is characterized by the gradual and irreversible replacement of healthy lung tissue with fibrous scar tissue, leading to decreased lung function, reduced oxygen exchange and critical respiratory deficiencies. Currently, predicting and managing lung fibrosis post-RT remains challenging, with limited preventive and treatment options. Accurate prediction of fibrosis onset and progression is therefore clinically crucial. We present a personalized in silico model for pulmonary fibrosis that encompasses tumour regression, fibrosis development and lung tissue remodelling post-radiation. Our continuum-based model was developed using data from 12 RT-treated lung cancer patients and integrates computed tomography (CT) and dosimetry data to simulate the spatio-temporal evolution of fibrosis. We demonstrate the ability of the in silico model to capture the extent of fibrosis in the entire cohort with a less than 1% deviation from clinical observations, in addition to providing quantitative metrics of spatial similarity. These findings underscore the potential of the model to improve treatment planning and risk assessment, paving the way for more personalized and effective management of RIPF.

High dose-rate brachytherapy presents a promising therapeutic avenue for prostate cancer management, involving the temporary implantation of catheters which deliver radioactive sources to the cancerous site. However, as catheters puncture and penetrate the prostate, tissue deformation is evident which may affect the accuracy and efficiency of the treatment. In this work, a data-driven in silico modelling procedure is proposed to simulate brachytherapy while accounting for prostate biomechanics. Comprehensive magnetic resonance and transrectal ultrasound images acquired prior, during and post brachytherapy are employed for model personalisation, while the therapeutic procedure is simulated via sequential insertion of multiple catheters in the prostate gland. The medical imaging data are also employed for model evaluation, thus, demonstrating the potential of the proposed in silico procedure to be utilised pre- and intra-operatively in the clinical setting., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2024 Hadjicharalambous, Roussakis, Bourantas, Ioannou, Miller, Doolan, Strouthos, Zamboglou and Vavourakis.)

The current study aimed to examine (a) whether the dominant leg (DL) was associated with the contralateral side of functional scoliosis and (b) if any of the postural asymmetries' evaluation variables may be a reliable predictor of the functional scoliosis development in young male soccer players. Six hundred-nine ( n = 609) male soccer players (age: 10.8 ± 2.7 years; height: 147 ± 17 cm; weight: 43.4 ± 14.6 kg; DL: Right 81.6%, Left 14%, Both 4.4%) participated in this study. The spinal asymmetries evaluation included thoracic kyphosis, lumbar lordosis, truncal rotation, shoulders alignment from posterior view, anterior and posterior pelvic tilt, anterior superior iliac spine (ASIS), hamstring tightness, and lower extremities discrepancy. A significant association was observed between the DL and the truncal rotation side: χ 2 (4) = 30.84, p = 0.001, V = 0.16. Participants with longer left legs were likelier to present a spinal asymmetry (OR = 1.18). The participants with higher left shoulders were 2.13 times more likely to have spinal asymmetry than the participants with normal shoulders level. Participants with left ASIS higher were 3.08 times more likely to present asymmetry than those with normally aligned ASIS levels. There was also a significant association between the DL and the side of truncal rotation: χ 2 (2) = 13.30, p = 0.001, V = 0.449. Logistic regression analysis for the functional scoliotic group and truncal rotation side demonstrated that the taller participants and participants with shorter right legs were more likely to have asymmetry on the left side (OR = 1.29, OR = 0.32). Participants with greater right hamstring stiffness were likelier to have a truncal rotation on the right side (OR = 0.93). Participants with higher left shoulders were 0.20 times less likely to have a truncal rotation on the left side than the participants with normal shoulders level. In conclusion, leg dominance in children and in youth soccer players may be a factor causing truncal rotation on the contralateral side. Additional causes, such as leg length discrepancy and pelvic tilt, may progressively lead to functional scoliosis.

Abstract: Kyriacou-Rossi, A, Hadjicharalambous, M, and Zaras, N. The influence of strength level on handball-specific fitness elements between stronger and weaker professional players. J Strength Cond Res 38(5): 966-975, 2024-The aim of the study was to investigate the influence of strength level between stronger and weaker handball players on handball specific fitness elements and to explore the correlations between strength and sport-specific fitness elements. Twenty-one professional male handball-players (age: 25.9 ± 6.9 years; mass: 87.9 ± 13.9 kg; height: 1.81 ± 0.08 m), participated in the study. Players were divided into the stronger (SG) and weaker group (WG) according to their relative to body mass 1 repetition maximum strength (1RM). Measurements included body composition, countermovement jump (CMJ), isometric leg extension peak torque (IPT) and rate of torque development (RTD), 5-step long-jump, 0-20 m linear sprint, T-half test, throwing velocity, and 1RM in bench press and squat. No significant difference was found for body composition between SG and WG ( p > 0.05). However, SG had significantly higher CMJ height (21.5%, p = 0.002), IPT (22.4%, p = 0.008), RTD relative to body mass ( p < 0.05), 5-step long jump (10.9%, p = 0.005), lower 0-20 linear sprint (-6.3%, p = 0.012), lower T-half test time trial (-7.3%, p = 0.001), and higher throwing velocity compared with WG ( p < 0.05). When all players included in one group, large to very large correlations were found between 1RM strength and IPT with fat-free mass ( r = 0.518-0.774) and throwing velocity ( r = 0.472-0.819). Very large correlations were found between RTD with fat-free mass ( r = 0.760) and throwing velocity ( r = 0.780-0.835). Stronger players have greater performance in all handball-fitness attributes compared with their weaker counterparts. The significant correlations between handball-specific fitness elements with strength and RTD suggest that strength training is essential for handball players as it may link to higher on court performance., (Copyright © 2023 National Strength and Conditioning Association.)

The purpose of this study was to investigate whether a dynamic warm-up either with weighted vests (WVs) or with resistance bands (RBs) may enhance the physical fitness and combat ability of kumite karate athletes. Nine male athletes (age range from 16 to 30 years), participated in this study. Athletes followed three different warm-up programs in a randomized order: (a) with their body mass only (C), (b) with a WV of 10% load of their body mass and (c) with a wearable RB of 10% tension of their body mass. Following the warm-up, athletes performed the following tests: countermovement jump (CMJ), standing long jump (LJ), punch and kick reaction time, bench press throws, T-Half test and the kick frequency test. CMJ remained unaltered following the WV and RB warm-up compared to the C. Standing long jump increased significantly following the RB warm-up compared to the C (2.2 ± 1.7%, p = 0.011). Punch reaction time was significantly shortened following the RB warm-up compared to C (-7.3 ± 5.9%, p = 0.019). No changes were observed for kick reaction time, bench press throws or the T-Half test. The kick decrement index, measured from the kick frequency test, was significantly decreased following the RB warm-up compared to the C (33.1 ± 20.2%, p = 0.011). These results suggest that an RB warm-up may significantly enhance the physical fitness and combat ability in kumite karate athletes.

The purpose of this study was to investigate the correlation between the reactive strength index (RSI) using the drop jump (DJ) and the isometric rate of torque development (RTD) with weightlifting performance in national-level weightlifters. Seven male weightlifters (age: 28.3 ± 5.7 years, body mass: 80.5 ± 6.7 kg, body height: 1.73 ± 0.07 m) participated in this study. Measurements were performed 2 weeks prior to the national championship and included the countermovement jump (CMJ), the squat jump (SJ), the DJ from three different drop heights (20, 30, and 40 cm), and the isometric peak torque (IPT) and RTD. Performance in CMJ and SJ was significantly correlated with weightlifting performance (r ranging from 0.756 to 0.892). Significant correlations were found between weightlifting performance with DJ contact time (r ranging from -0.759 to -0.899) and RSI (r ranging from 0.790 to 0.922). Moreover, the best RSI was significantly correlated with the snatch (r = 0.921, p = 0.003) and total performance (r = 0.832, p = 0.020). Small to very large correlations were found between IPT and RTD with weightlifting performance (r ranging from 0.254 to 0.796). These results suggest that RSI and contact time variables from DJ may predict weightlifting performance in well-trained weightlifters. Additionally, IPT and RTD may provide useful insights into the neuromuscular fitness condition of the weightlifter.

Introduction: Pilates-mat is an effective training method to enhance several physiological and fitness parameters, although the effects of pilates-mat detraining on body composition and physical fitness remains largely unknown. The purpose of the study was to investigate the effect of pilates-mat training and detraining on body composition and physical fitness in pilates-trained females., Methods: Twenty females (age: 45.1 ± 8.7 years) followed an 8-week pilates-mat training program followed by a 3-week detraining period. Measurements performed at the beginning of the training intervention, at the end of the 8-week training program and at the end of the 3-week detraining period and included: anthropometric characteristics, body composition analysis, thigh, hip and waist circumferences, blood pressure, heart rate at rest (HRr), maximum handgrip strength, abdominal curls, lower body flexibility and aerobic capacity., Results: Pilates-mat training significantly decreased body fat percentage (BF%), visceral fat and HRr (p < 0.05) but these variables returned to baseline after detraining. Trunk fat, thigh and waist circumferences decreased significantly after training and remained significantly low following detraining (p < 0.05). Abdominal curls, lower body flexibility and aerobic capacity increased significantly following pilates-mat training and remained unchanged after detraining (p < 0.05)., Conclusions: These results suggest that 8 weeks of pilates-mat training enhanced body composition and physical fitness and these physiological and physical fitness benefits were remained unaltered during the 3-week of pilates-mat detraining period. However, BF% and visceral fat returned to baseline levels following the detraining period suggesting that for maintaining the reduction in BF% and visceral fat a long-period of pilates-mat detraining should be avoided., Competing Interests: Declaration of competing interest Nothing to declare., (Copyright © 2023 Elsevier Ltd. All rights reserved.)

Purpose: Research on whether caffeine habituation reduces its ergogenicity is scarce and conflicting. The purpose of the present study was to examine the influence of habitual caffeine consumption on exercise performance and biological responses during a simulated soccer-game protocol following acute caffeine ingestion., Methods: Twenty professional male soccer players were categorized as higher (n = 9) or lower caffeine consumers (n = 11) after answering a validated questionnaire. Participants performed a simulated treadmill soccer-game protocol on treadmill following either caffeine (6 mg kg -1 ) or placebo ingestion, during which several variables were evaluated., Results: Time to exhaustion, countermovement jump height, mean arterial pressure, heart rate, plasma glucose, and lactate were higher (P ≤ 0.001), while rating of perceived exertion (RPE) was lower (P = 0.002), following caffeine compared to placebo ingestion, with no differences between groups (P > 0.05). Plasma non-esterified fatty acids exhibited a higher response to caffeine in the higher vs lower caffeine consumers. Reaction time, plasma glycerol and epinephrine, carbohydrate and fat oxidation, and energy expenditure were not affected by caffeine (P > 0.05)., Conclusion: Caffeine ingestion largely improved cardiovascular and neuromuscular performance, while reducing RPE, in both higher and lower caffeine consuming athletes during prolonged intermitted exercise to exhaustion., (© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany.)

Anti-angiogenic (AA) treatments have received significant research interest due to the key role of angiogenesis in cancer progression. AA agents can have a strong effect on cancer regression, by blocking new vessels and reducing the density of the existing vasculature. Moreover, in a process termed vascular normalisation, AA drugs can improve the abnormal structure and function of the tumour vasculature, enhancing the delivery of chemotherapeutics to the tumour site. Despite their promising potential, an improved understanding of AA treatments is necessary to optimise their administration as a monotherapy or in combination with other cancer treatments. In this work we present an in silico multiscale cancer model which is used to systematically interrogate the role of individual mechanisms of action of AA drugs in tumour regression. Focus is placed on the reduction of vascular density and on vascular normalisation through a parametric study, which are considered either as monotherapies or in combination with conventional/ metronomic chemotherapy. The model is specified to data from a mammary carcinoma xenograft in immunodeficient mice, to enhance the physiological relevance of model predictions. Our results suggest that conventional chemotherapy might be more beneficial when combined with AA treatments, hindering tumour growth without causing excessive damage on healthy tissue. Notably, metronomic chemotherapy has shown significant potential in stopping tumour growth with minimal toxicity, even as a monotherapy. Our findings underpin the potential of our in silico framework for non-invasive and cost-effective evaluation of treatment strategies, which can enhance our understanding of combined therapeutic strategies and contribute towards improving cancer treatment management., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

Ventricular-vascular interaction is central in the adaptation to cardiovascular disease. However, cardiomyopathy patients are predominantly monitored using cardiac biomarkers. The aim of this study is therefore to explore aortic function in dilated cardiomyopathy (DCM). Fourteen idiopathic DCM patients and 16 controls underwent cardiac magnetic resonance imaging, with aortic relative pressure derived using physics-based image processing and a virtual cohort utilized to assess the impact of cardiovascular properties on aortic behaviour. Subjects with reduced left ventricular systolic function had significantly reduced aortic relative pressure, increased aortic stiffness, and significantly delayed time-to-pressure peak duration. From the virtual cohort, aortic stiffness and aortic volumetric size were identified as key determinants of aortic relative pressure. As such, this study shows how advanced flow imaging and aortic hemodynamic evaluation could provide novel insights into the manifestation of DCM, with signs of both altered aortic structure and function derived in DCM using our proposed imaging protocol., (© 2021. The Author(s).)

The purpose of the study was to investigate the effect of dry-land priming (DLP) versus swimming priming (SP) on the 50 m crawl performance of well-trained adolescent swimmers. Thirteen adolescent swimmers were randomly assigned to perform either a DLP or SP 24 h prior to a 50 m sprint crawl time-trial. Baseline measurements included a 50 m sprint crawl time-trial as a control (C) condition, the evaluation of body composition, countermovement jump (CMJ), isometric peak torque (IPT), and rate of torque development (RTD). Rating of perceived exertion (RPE) was obtained following the DLP and SP programs. Both DLP and SP significantly decreased the 50 m crawl time-trial, by −2.51 ± 2.43% and −2.59 ± 1.89% (p < 0.01), respectively, compared with the C time-trial. RPE was not different between DLP and SP (p = 0.919). CMJ performance remained unchanged after DLP and SP programs compared with the C trial (p > 0.05). The percentage decrease in the 50 m crawl after DLP was significantly correlated with the percentage decrease in the 50 m crawl following SP (r = 0.720, p = 0.006). CMJ power, lean body mass, IPT, and RTD were significantly correlated with 50 m crawl performance. These results suggest that both DLP and SP strategies, when applied 24 h prior to a 50 m crawl time-trial, may enhance performance in well-trained adolescent swimmers.

The effectiveness of chemotherapy in cancer cell regression is often limited by drug resistance, toxicity, and neoplasia heterogeneity. However, due to the significant complexities entailed by the many cancer growth processes, predicting the impact of interference and symmetry-breaking mechanisms is a difficult problem. To quantify and understand more about cancer drug pharmacodynamics, we combine in vitro with in silico cancer models. The anti-proliferative action of selected cytostatics is interrogated on human colorectal and breast adenocarcinoma cells, while an agent-based computational model is employed to reproduce experiments and shed light on the main therapeutic mechanisms of each chemotherapeutic agent. Multiple drug administration scenarios on each cancer cell line are simulated by varying the drug concentration, while a Bayesian-based method for model parameter optimisation is employed. Our proposed procedure of combining in vitro cancer drug screening with an in silico agent-based model successfully reproduces the impact of chemotherapeutic drugs in cancer growth behaviour, while the mechanisms of action of each drug are characterised through model-derived probabilities of cell apoptosis and division. We suggest that our approach could form the basis for the prospective generation of experimentally-derived and model-optimised pharmacological variables towards personalised cancer therapy.

The purpose of the study was to examine the effect of seven-week inter-repetition rest vs. traditional resistance training on upper body maximum strength, the rate of force development and triceps brachii muscle architecture. Sixteen male participants were equally assigned into the inter-repetition rest and the traditional group. In both groups, training included the bench press exercise performed with 4 sets of 6 maximum repetitions, two training sessions per week. Twenty-second inter-repetition rest was employed for the inter-repetition rest group only. Measurements before and after the training period included maximum strength in the bench press, the isometric upper body rate of force development and peak force and triceps brachii muscle architecture. Maximum strength increased significantly in both groups (inter-repetition rest group: 21.5 ± 5.7% vs. traditional group: 13.5 ± 7.2%, p < 0.05), however, the maximum strength percentage increase was greater in the inter-repetition rest group compared to the traditional group (p = 0.027). Upper body isometric peak force increased only after inter-repetition rest training (10.7 ± 10.3%, p = 0.009). The rate of force development remained unchanged for both groups (p > 0.05), although percentage changes in time frames of 0-80 and 0-100 milliseconds were greater for the inter-repetition rest group compared to the traditional training group (p = 0.024 and p = 0.044, respectively). Triceps brachii thickness increased similarly for both groups (p < 0.05). These results suggest that inter-repetition rest may induce greater increases in maximum strength and the rate of force development compared to traditional training during the initial weeks of resistance training., (© 2022 Nikolaos Zaras, Angeliki-Nikoletta Stasinaki, Thomas Mpampoulis, Polyxeni Spiliopoulou, Marios Hadjicharalambous, Gerasimos Terzis, published by Sciendo.)

Pseudomonas citronellolis SJTE-3 was isolated as a highly efficient microorganism for biodegradation and valorization of drilling fluids (DF) wastewater. The strain metabolised DF and oily mud exhibiting up to 93%, 86%, 85% and 88% of chemical oxygen demand (COD), n-dodecane, n-tetradecane and naphthalene removal efficiency respectively. Enhanced bioconversion was enabled through production of biosurfactants that reduced the surface tension of water by 53% and resulted in 43.3% emulsification index (E24), while synthesizing 24% of dry cell weight (DCW) as medium-chain-length polyhydroxyalkanoates (PHA). Expression from the main pathways for alkanes and naphthalene biodegradation as well as biosurfactants and PHA biosynthesis revealed that although the alkanes and naphthalene biodegradation routes were actively expressed even at stationary phase, PHA production was stimulated at late stationary phase and putisolvin could comprise the biosurfactant synthesized. The bioconversion of toxic petrochemical residues to added-value thermoelastomers and biosurfactants indicate the high industrial significance of P. citronellolis SJTE-3., (Copyright © 2021 Elsevier Ltd. All rights reserved.)

Understanding the biomechanics of the heart in health and disease plays an important role in the diagnosis and treatment of heart failure. The use of computational biomechanical models for therapy assessment is paving the way for personalized treatment, and relies on accurate constitutive equations mapping strain to stress. Current state-of-the art constitutive equations account for the nonlinear anisotropic stress-strain response of cardiac muscle using hyperelasticity theory. While providing a solid foundation for understanding the biomechanics of heart tissue, most current laws neglect viscoelastic phenomena observed experimentally. Utilizing experimental data from human myocardium and knowledge of the hierarchical structure of heart muscle, we present a fractional nonlinear anisotropic viscoelastic constitutive model. The model is shown to replicate biaxial stretch, triaxial cyclic shear and triaxial stress relaxation experiments (mean error ∼7.68%), showing improvements compared to its hyperelastic (mean error ∼24%) counterparts. Model sensitivity, fidelity and parameter uniqueness are demonstrated. The model is also compared to rate-dependent biaxial stretch as well as different modes of biaxial stretch, illustrating extensibility of the model to a range of loading phenomena. STATEMENT OF SIGNIFICANCE: The viscoelastic response of human heart tissues has yet to be integrated into common constitutive models describing cardiac mechanics. In this work, a fractional viscoelastic modeling approach is introduced based on the hierarchical structure of heart tissue. From these foundations, the current state-of-the-art biomechanical models of the heart muscle are transformed using fractional viscoelasticity, replicating passive muscle function across multiple experimental tests. Comparisons are drawn with current models to highlight the improvements of this approach and predictive responses show strong qualitative agreement with experimental data. The proposed model presents the first constitutive model aimed at capturing viscoelastic nonlinear response across multiple testing regimes, providing a platform for better understanding the biomechanics of myocardial tissue in health and disease., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2021 The Authors. Published by Elsevier Ltd.. All rights reserved.)

A major concern in personalised models of heart mechanics is the unknown zero-pressure domain, a prerequisite for accurately predicting cardiac biomechanics. As the reference configuration cannot be captured by clinical data, studies often employ in-vivo frames which are unlikely to correspond to unloaded geometries. Alternatively, zero-pressure domain is approximated through inverse methodologies, which, however, entail assumptions pertaining to boundary conditions and material parameters. Both approaches are likely to introduce biases in estimated biomechanical properties; nevertheless, quantification of these effects is unattainable without ground-truth data. In this work, we assess the unloaded state influence on model-derived biomechanics, by employing an in-silico modelling framework relying on experimental data on porcine hearts. In-vivo images are used for model personalisation, while in-situ experiments provide a reliable approximation of the reference domain, creating a unique opportunity for a validation study. Personalised whole-cycle cardiac models are developed which employ different reference domains (image-derived, inversely estimated) and are compared against ground-truth model outcomes. Simulations are conducted with varying boundary conditions, to investigate the effect of data-derived constraints on model accuracy. Attention is given to modelling the influence of the ribcage on the epicardium, due to its close proximity to the heart in the porcine anatomy. Our results find merit in both approaches for dealing with the unknown reference domain, but also demonstrate differences in estimated biomechanical quantities such as material parameters, strains and stresses. Notably, they highlight the importance of a boundary condition accounting for the constraining influence of the ribcage, in forward and inverse biomechanical models., (© 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

Solid tumour growth is often associated with the accumulation of mechanical stresses acting on the surrounding host tissue. Due to tissue nonlinearity, the shear modulus of the peri-tumoural region inherits a signature from the tumour expansion which depends on multiple factors, including the soft tissue constitutive behaviour and its stress/strain state. Shear waves used in MR-elastography (MRE) sense the apparent change in shear modulus along their propagation direction, thereby probing the anisotropic stiffness field around the tumour. We developed an analytical framework for a heterogeneous shear modulus distribution using a thick-shelled sphere approximation of the tumour and soft tissue ensemble. A hyperelastic material (plastisol) was identified to validate the proposed theory in a phantom setting. A balloon-catheter connected to a pressure sensor was used to replicate the stress generated from tumour pressure and growth while MRE data were acquired. The shear modulus anisotropy retrieved from the reconstructed elastography data confirmed the analytically predicted patterns at various levels of inflation. An alternative measure, combining the generated deformation and the local wave direction and independent of the reconstruction strategy, was also proposed to correlate the analytical findings with the stretch probed by the waves. Overall, this work demonstrates that MRE in combination with non-linear mechanics, is able to identify the apparent shear modulus variation arising from the strain generated by a growth within tissue, such as an idealised model of tumour. Investigation in real tissue represents the next step to further investigate the implications of endogenous forces in tissue characterisation through MRE., Competing Interests: The authors have declared that no competing interests exist.

Objective: Both low-load-high-repetitions (LLHR) and Pilates programs constitute popular forms of exercise, accompanied by health benefits for the participants involved. Notably, the effect of such programs on aerobic fitness is still controversial. The aim of this study was to examine the effects of both programs on physical fitness and body composition on previously inactive adult women., Methods: Twenty-six women (39.8 ± 9.1y) were assigned to a LLHR program, and sixteen women (39.1 ± 12.2y) were assigned to a Pilates program. Both programs were performed in a group setting, 3 times per week for 3 months. Aerobic fitness, flexibility, handgrip strength and lower extremities explosiveness were assessed by a battery of field testing. Total body fat and trunk fat levels were assessed by bioelectrical impedance analysis. Heart rate response during exercise was recorded once every month by using a telemetry system., Results: Aerobic fitness, lower extremities explosive power, left arm handgrip strength and body composition significantly improved in the LLHR group; while flexibility significantly improved only in the Pilates group, following the intervention period (p < 0.05). LLHR was superior to the Pilates program in improving aerobic fitness and body composition; whilst Pilates was superior in improving flexibility (p < 0.05)., Conclusion: LLHR group-based exercise programs may improve various aspects of physical fitness, including aerobic fitness, in inactive adult women. This medium-intensity form of exercise is generally well tolerated and might be used as an option for women who cannot perform training on higher intensities. In contrast, the Pilates program failed to improve physical fitness-related parameters except flexibility levels., (Copyright © 2020 Elsevier Ltd. All rights reserved.)

Introduction: Innovations in 3D spatial technology and augmented reality imaging driven by digital high-tech industrial science have accelerated experimental advances in breast cancer imaging and the development of medical procedures aimed to reduce invasiveness., Presentation of Case: A 57-year-old post-menopausal woman presented with screen-detected left-sided breast cancer. After undergoing all staging and pre-operative studies the patient was proposed for conservative breast surgery with tumor localization. During surgery, an experimental digital and non-invasive intra-operative localization method with augmented reality was compared with the standard pre-operative localization with carbon tattooing (institutional protocol). The breast surgeon wearing an augmented reality headset (Hololens) was able to visualize the tumor location projection inside the patient's left breast in the usual supine position., Discussion: This work describes, to our knowledge, the first experimental test with a digital non-invasive method for intra-operative breast cancer localization using augmented reality to guide breast conservative surgery. In this case, a successful overlap of the previous standard pre-operative marks with carbon tattooing and tumor visualization inside the patient's breast with augmented reality was obtained., Conclusion: Breast cancer conservative guided surgery with augmented reality can pave the way for a digital non-invasive method for intra-operative tumor localization., Competing Interests: Declaration of competing interest No conflict of interest and financial relationships relevant to the content of this article have been disclosed by any of the other authors., (Copyright © 2021 The Author(s). Published by Elsevier Ltd.. All rights reserved.)

Purpose: The purpose of the present study was to investigate the relationship between weightlifting performance and the rate of force development (RFD), muscle architecture, and body composition in elite Olympic weightlifters., Methods: Six male Olympic weightlifters (age 23.3 [3.4] y, body mass 88.7 [10.2] kg, body height 1.76 [0.07] m, snatch 146.7 [15.4] kg, clean and jerk 179.4 [22.1] kg), all members of the national team, participated in the study. Athletes completed a 16-week periodized training program aiming to maximize their performance at the national competition event. Measurements, including maximal strength (1-repetition maximum) in snatch, clean and jerk, back and front squat, isometric leg press RFD and peak force, countermovement jump, vastus lateralis muscle architecture, and body composition, were performed before and after the training period., Results: Weightlifting performance increased significantly after training (P < .05). Leg press RFD increased only in time windows of 0 to 200 and 0 to 250 milliseconds after training (8.9% [8.5%] and 9.4% [7.7%], respectively, P < .05) while peak force remained unaltered (P < .05). Front squat strength increased significantly (P < .05), while countermovement jump power increased 2.3% (2.1%) (P < .05). No changes were observed for muscle architecture and lean body mass (P > .05). Significant correlations were observed between performance in snatch and clean and jerk with isometric leg press RFD, at all time windows, as well as with lean body mass and squat 1-repetition maximum., Conclusions: These results suggest that regular examination of RFD, lean body mass, and lower extremities' 1-repetition maximum may be useful performance predictors in elite Olympic weightlifters.

In silico cancer models have demonstrated great potential as a tool to improve drug design, optimise the delivery of drugs to target sites in the host tissue and, hence, improve therapeutic efficacy and patient outcome. However, there are significant barriers to the successful translation of in silico technology from bench to bedside. More precisely, the specification of unknown model parameters, the necessity for models to adequately reflect in vivo conditions, and the limited amount of pertinent validation data to evaluate models' accuracy and assess their reliability, pose major obstacles in the path towards their clinical translation. This review aims to capture the state-of-the-art in in silico cancer modelling of vascularised solid tumour growth, and identify the important advances and barriers to success of these models in clinical oncology. Particular emphasis has been put on continuum-based models of cancer since they - amongst the class of mechanistic spatio-temporal modelling approaches - are well-established in simulating transport phenomena and the biomechanics of tissues, and have demonstrated potential for clinical translation. Three important avenues in in silico modelling are considered in this contribution: first, since systemic therapy is a major cancer treatment approach, we start with an overview of the tumour perfusion and angiogenesis in silico models. Next, we present the state-of-the-art in silico work encompassing the delivery of chemotherapeutic agents to cancer nanomedicines through the bloodstream, and then review continuum-based modelling approaches that demonstrate great promise for successful clinical translation. We conclude with a discussion of what we view to be the key challenges and opportunities for in silico modelling in personalised and precision medicine., (Copyright © 2020 Elsevier Inc. All rights reserved.)

Background: Previous evidence has demonstrated that serum leptin is correlated with appetite in combination with, but not without, modest exercise., Aim: The present experiments investigated the effects of exogenous adrenaline and α/β adrenoceptor blockade in combination with moderate exercise on serum leptin concentrations, appetite/satiety sensations and subsequent food intake in obese women., Methods: A total of 10 obese women ((mean ± SEM), age: 50 (1.9) years, body mass index 36 (4.1) kg/m 2 , waist 104.8 (4.1) cm) participated in two separate, double-blind randomised experimental trials. Experiment 1: moderate exercise after α/β adrenergic blocker (labetalol, 100 mg orally) versus moderate exercise plus placebo; experiment 2: adrenaline infusion for 20 minutes versus saline infusion. Appetite/satiety and biochemistry were measured at baseline, pre- and immediately post-intervention, then 1 hour post-intervention (i.e., before dinner). Food intake was assessed via ad libitum buffet-style dinner., Results: No differences were found in appetite/satiety, subsequent food intake or serum leptin in any of the studies (experiment 1 or experiment 2). In experiment 1, blood glucose was higher ( p < 0.01) and plasma free fatty acids lower ( p = 0.04) versus placebo. In experiment 2, plasma free fatty acids ( p < 0.05) increased after adrenaline versus saline infusion., Conclusions: Neither inhibition of exercise-induced adrenergic activity by combined α/β adrenergic blockade nor moderate increases in adrenergic activity induced by intravenous adrenaline infusion affected acute appetite regulation.

Background: Equivocal findings examining the influence of caffeine on performance and biological responses to exercise may be due to inter-individual variability in cardiorespiratory or neuromuscular fitness. This study examined whether the effects of caffeine ingestion on exercise performance and biological responses to prolonged intermittent exercise to exhaustion depend on cardiorespiratory or neuromuscular fitness., Methods: Twenty male soccer players, separated according to either cardiorespiratory fitness (high vs medium) or neuromuscular fitness (high vs medium) underwent two trials simulating the cardiovascular demands of a soccer game to exhaustion on treadmill after ingesting either caffeine (6 mg∙kg - 1 ) or placebo. Physical performance, cardiorespiratory and metabolic parameters and blood metabolites were evaluated., Results: Time to exhaustion (719 ± 288 vs 469 ± 228 s), jump height (42.7 ± 4.2 vs 38.6 ± 4.4 cm), heart rate (163 ± 12 vs 157 ± 13 b∙min - 1 ), mean arterial blood pressure (98 ± 8 vs 92 ± 10 mmHg), plasma glucose (5.6 ± 0.7 vs 5.3 ± 0.6 mmol∙l - 1 ) and lactate (3.3 ± 1.2 vs 2.9 ± 1.2 mmol∙l - 1 ) were higher, while rating of perceived exertion (12.6 ± 1.7 vs 13.3 ± 1.6) was lower with caffeine vs placebo (p < 0.01), independent of cardiorespiratory or neuromuscular fitness level. Reaction time; plasma glycerol, non-esterified fatty acids and epinephrine; carbohydrate and fat oxidation rates; and energy expenditure were not affected by caffeine (p > 0.05)., Conclusions: Caffeine was effective in improving endurance and neuromuscular performance in athletes with either high or medium cardiorespiratory and neuromuscular fitness. Cardiorespiratory and neuromuscular fitness do not appear to modulate the ergogenic effects of caffeine supplementation in well-trained athletes.