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Abstract Background Microalgae hold promise for yielding a biofuel feedstock that is sustainable, carbon-neutral, distributed, and only minimally disruptive for the production of food and feed by traditional agriculture. Amongst oleaginous eukaryotic algae, the B race of Botryococcus braunii is unique in that it produces large amounts of liquid hydrocarbons of terpenoid origin. These are comparable to fossil crude oil, and are sequestered outside the cells in a communal extracellular polymeric matrix material. Biosynthetic engineering of terpenoid bio-crude production requires identification of genes and reconstruction of metabolic pathways responsible for production of both hydrocarbons and other metabolites of the alga that compete for photosynthetic carbon and energy. Results A de novo assembly of 1,334,609 next-generation pyrosequencing reads form the Showa strain of the B race of B. braunii yielded a transcriptomic database of 46,422 contigs with an average length of 756 bp. Contigs were annotated with pathway, ontology, and protein domain identifiers. Manual curation allowed the reconstruction of pathways that produce terpenoid liquid hydrocarbons from primary metabolites, and pathways that divert photosynthetic carbon into tetraterpenoid carotenoids, diterpenoids, and the prenyl chains of meroterpenoid quinones and chlorophyll. Inventories of machine-assembled contigs are also presented for reconstructed pathways for the biosynthesis of competing storage compounds including triacylglycerol and starch. Regeneration of S-adenosylmethionine, and the extracellular localization of the hydrocarbon oils by active transport and possibly autophagy are also investigated. Conclusions The construction of an annotated transcriptomic database, publicly available in a web-based data depository and annotation tool, provides a foundation for metabolic pathway and network reconstruction, and facilitates further omics studies in the absence of a genome sequence for the Showa strain of B. braunii, race B. Further, the transcriptome database empowers future biosynthetic engineering approaches for strain improvement and the transfer of desirable traits to heterologous hosts.

This review presents the algal diversity and their evolution through endosymbiotic process. Research findings indicate that a previously non-photosynthetic protist gave rise to eukaryotic algae through endocytosis of a procaryotic cyanobacterium. The role of endosymbiosis and the exent of endosymbiotic gene transfer are discussed.

The image of Roger Bacon as a `modern' experimental scientist was propagated as historical truth in 19th century scientific historiography. Twentieth century criticisms attacked this tradition, arguing that Bacon was primarily a medieval philosopher with `medieval' scientific interests. However, recent scholarship has provided a more careful and critical account of Bacon's science, and identifies his greatest achievement in terms of his successful attempt to assimilate the worlds of Greek and Islamic optics. It can be justly claimed that Roger Bacon was the first Western thinker in the middle ages to have mastered most of the Greek sources and the central Islamic source in optics. He made this scientific domain understandable for a Western Latin-reading audience. Yet, Bacon himself acknowledged Adelard of Bath, whose translations and commentary of Euclid's Elements set the foundations for a science of optics, as the true pioneer.

Author(s): Nicole T. Perna (corresponding author) [1, 2]; Guy Plunkett, III [3]; Valerie Burland [3]; Bob Mau [3]; Jeremy D. Glasner [3]; Debra J. Rose [3]; George F. Mayhew [3]; [...]