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1. Generation of nanobodies from transgenic ‘LamaMice’ lacking an endogenous immunoglobulin repertoire

Author

Eden, Thomas, Schaffrath, Alessa Z., Wesolowski, Janusz, Stähler, Tobias, Tode, Natalie, Richter, Nathalie, Schäfer, Waldemar, Hambach, Julia, Hermans-Borgmeyer, Irm, Woens, Jannis, Le Gall, Camille M., Wendler, Sabrina, Linke-Winnebeck, Christian, Stobbe, Martina, Budnicki, Iwona, Wanney, Amelie, Heitz, Yannic, Schimmelpfennig, Lena, Schweitzer, Laura, Zimmer, Dennis, Stahl, Erik, Seyfried, Fabienne, Gebhardt, Anna J., Dieckow, Lynn, Riecken, Kristoffer, Fehse, Boris, Bannas, Peter, Magnus, Tim, Verdoes, Martijn, Figdor, Carl G., Hartlepp, Klaus F., Schleer, Hubertus, Füner, Jonas, Tomas, Nicola M., Haag, Friedrich, Rissiek, Björn, Mann, Anna M., Menzel, Stephan, and Koch-Nolte, Friedrich

Published
2024
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5. BCMA x CD3 T-cell engager in a patient with penta-refractory multiple myeloma and HIV: a clinical and immunological report

Author

Cords, Leon, primary, Schaefers, Christoph, additional, Kamili, Abdulaziz, additional, Hoffmann, Christian, additional, Cichutek, Sophia, additional, Haag, Friedrich, additional, Polywka, Susanne, additional, Bokemeyer, Carsten, additional, Leypoldt, Lisa, additional, Alsdorf, Winfried, additional, Wiesch, Julian Schulze zur, additional, and Weisel, Katja C., additional

Published
2024
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6. Generation and characterization of antagonistic anti-human CD39 nanobodies

Author

Menzel, Stephan, primary, Duan, Yinghui, additional, Hambach, Julia, additional, Albrecht, Birte, additional, Wendt-Cousin, Dorte, additional, Winzer, Riekje, additional, Tolosa, Eva, additional, Rissiek, Anne, additional, Guse, Andreas H., additional, Haag, Friedrich, additional, Magnus, Tim, additional, Koch-Nolte, Friedrich, additional, and Rissiek, Björn, additional

Published
2024
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7. Multi-dimensional and longitudinal systems profiling reveals predictive pattern of severe COVID-19

Author

Woo, Marcel S., Haag, Friedrich, Nierhaus, Axel, Jarczak, Dominik, Roedl, Kevin, Mayer, Christina, Brehm, Thomas T., van der Meirschen, Marc, Hennigs, Annette, Christopeit, Maximilian, Fiedler, Walter, Karagiannis, Panagiotis, Burdelski, Christoph, Schultze, Alexander, Huber, Samuel, Addo, Marylyn M., Schmiedel, Stefan, Friese, Manuel A., Kluge, Stefan, and Schulze zur Wiesch, Julian

Published
2021
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8. Development of Antibody and Nanobody Tools for P2X7

Author

Stähler, Tobias, primary, Danquah, Welbeck, additional, Demeules, Melanie, additional, Gondé, Henri, additional, Hardet, Romain, additional, Haag, Friedrich, additional, Adriouch, Sahil, additional, Koch-Nolte, Friedrich, additional, and Menzel, Stephan, additional

Published
2022
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12. Generation and characterization of antagonistic anti-human CD39 nanobodies.

Author

Duan, Stephan Menzel Yinghui, Hambach, Julia, Albrecht, Birte, Wendt-Cousin, Dorte, Winzer, Riekje, Tolosa, Eva, Rissiek, Anne, Guse, Andreas H., Haag, Friedrich, Magnus, Tim, Koch-Nolte, Friedrich, and Rissiek, Björn

Subjects
IMMUNOGLOBULINS, ADENOSINE monophosphate, ADENOSINE diphosphate, ADENOSINE triphosphate, MONOCLONAL antibodies
Abstract

CD39 is the major enzyme controlling the levels of extracellular adenosine triphosphate (ATP) via the stepwise hydrolysis of ATP to adenosine diphosphate (ADP) and adenosine monophosphate (AMP). As extracellular ATP is a strong promoter of inflammation, monoclonal antibodies (mAbs) blocking CD39 are utilized therapeutically in the field of immune-oncology. Though anti- CD39 mAbs are highly specific for their target, they lack deep penetration into the dense tissue of solid tumors, due to their large size. To overcome this limitation, we generated and characterized nanobodies that targeted and blocked human CD39. From cDNA-immunized alpacas we selected 16 clones from seven nanobody families that bind to two distinct epitopes of human CD39. Among these, clone SB24 inhibited the enzymatic activity of CD39. Of note, SB24 blocked ATP degradation by both soluble and cell surface CD39 as a 15kD monomeric nanobody. Dimerization via fusion to an immunoglobulin Fc portion further increased the blocking potency of SB24 on CD39-transfected HEK cells. Finally, we confirmed the CD39 blocking properties of SB24 on human PBMCs. In summary, SB24 provides a new small biological antagonist of human CD39 with potential application in cancer therapy. [ABSTRACT FROM AUTHOR]

Published
2024
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13. Immunosuppressive M2 TAMs represent a promising target population to enhance phagocytosis of ovarian cancer cells in vitro

Author

Brauneck, Franziska, primary, Oliveira-Ferrer, Leticia, additional, Muschhammer, Jana, additional, Sturmheit, Tabea, additional, Ackermann, Christin, additional, Haag, Friedrich, additional, Schulze zur Wiesch, Julian, additional, Ding, Yi, additional, Qi, Minyue, additional, Hell, Louisa, additional, Schmalfeldt, Barbara, additional, Bokemeyer, Carsten, additional, Fiedler, Walter, additional, and Wellbrock, Jasmin, additional

Published
2023
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20. ADP-Ribosylation of P2X7: A Matter of Life and Death for Regulatory T Cells and Natural Killer T Cells

Author

Rissiek, Björn, Haag, Friedrich, Boyer, Olivier, Koch-Nolte, Friedrich, Adriouch, Sahil, Compans, Richard W, Series editor, Cooper, Max D., Series editor, Gleba, Yuri Y., Series editor, Honjo, Tasuku, Series editor, Oldstone, Michael B. A., Series editor, Vogt, Peter K., Series editor, Malissen, Bernard, Series editor, Aktories, Klaus, Series editor, Kawaoka, Yoshihiro, Series editor, Rappuoli, Rino, Series editor, Galan, Jorge E., Series editor, Ahmed, Rafi, Series editor, and Koch-Nolte, Friedrich, editor

Published
2015
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21. Identification and Analysis of ADP-Ribosylated Proteins

Author

Haag, Friedrich, Buck, Friedrich, Compans, Richard W, Series editor, Cooper, Max D., Series editor, Gleba, Yuri Y., Series editor, Honjo, Tasuku, Series editor, Oldstone, Michael B. A., Series editor, Vogt, Peter K., Series editor, Malissen, Bernard, Series editor, Aktories, Klaus, Series editor, Kawaoka, Yoshihiro, Series editor, Rappuoli, Rino, Series editor, Galan, Jorge E., Series editor, Ahmed, Rafi, Series editor, and Koch-Nolte, Friedrich, editor

Published
2015
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22. A transplant “immunome” screening platform defines a targetable epitope fingerprint of multiple myeloma

Author

Schieferdecker, Aneta, Oberle, Anna, Thiele, Benjamin, Hofmann, Fabian, Göthel, Markus, Miethe, Sebastian, Hust, Michael, Braig, Friederike, Voigt, Mareike, von Pein, Ute-Marie, Koch-Nolte, Friedrich, Haag, Friedrich, Alawi, Malik, Indenbirken, Daniela, Grundhoff, Adam, Bokemeyer, Carsten, Bacher, Ulrike, Kröger, Nicolaus, and Binder, Mascha

Published
2016
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25. TIGIT blockade repolarizes AML-associated TIGIT+M2 macrophages to an M1 phenotype and increases CD47-mediated phagocytosis

Author

Brauneck, Franziska, primary, Fischer, Brit, additional, Witt, Marius, additional, Muschhammer, Jana, additional, Oelrich, Jennyfer, additional, da Costa Avelar, Pedro Henrique, additional, Tsoka, Sophia, additional, Bullinger, Lars, additional, Seubert, Elisa, additional, Smit, Daniel J, additional, Bokemeyer, Carsten, additional, Ackermann, Christin, additional, Wellbrock, Jasmin, additional, Haag, Friedrich, additional, and Fiedler, Walter, additional

Published
2022
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26. A simple, sensitive, and low‐cost FACS assay for detecting antibodies against the native SARS‐CoV‐2 spike protein

Author

Hambach, Julia, Stähler, Tobias, Eden, Thomas, Wendt, Dorte, Tode, Natalie, Haag, Friedrich, Tolosa, Eva, Altfeld, Marcus, Fathi, Anahita, Dahlke, Christine, Addo, Marylyn M., Menzel, Stephan, and Koch‐Nolte, Friedrich

Subjects
Adult, Aged, 80 and over, SARS-CoV-2, flow cytometry, COVID-19, Original Articles, Middle Aged, RC581-607, Antibodies, Viral, infectious diseases, virology, Cricetulus, HEK293 Cells, Cricetinae, Spike Glycoprotein, Coronavirus, Animals, Humans, antibodies, Original Article, Immunologic diseases. Allergy, Aged
Abstract

Background: Hamburg is a city state of approximately 1.9 Mio inhabitants in Northern Germany. Currently, the COVID‐19 epidemic that had largely subsided during last summer is resurging in Hamburg and in other parts of the world, underlining the need for additional tools to monitor SARS‐CoV‐2 antibody responses. Aim: We aimed to develop and validate a simple, low‐cost assay for detecting antibodies against the native coronavirus 2 spike protein (CoV‐2 S) that does not require recombinant protein or virus. Method: We transiently co‐transfected HEK cells or CHO cells with expression vectors encoding CoV‐2 S and nuclear GFP. Spike protein‐specific antibodies in human serum samples bound to transfected cells were detected with fluorochrome conjugated secondary antibodies by flow cytometry orimmunofluorescence microscopy. We applied this assay to monitor antibody development in COVID‐19 patients, household contacts, and hospital personnel during the ongoing epidemic in the city state of Hamburg. Results: All recovered COVID‐19 patients showed high levels of CoV‐2 S‐specific antibodies. With one exception, all household members that did not develop symptoms also did not develop detectable antibodies. Similarly, lab personnel that worked during the epidemic and followed social distancing guidelines remained antibody‐negative. Conclusion: We conclude that high‐titer CoV‐2 S‐specific antibodies are found in most recovered COVID‐19 patients and in symptomatic contacts, but only rarely in asymptomatic contacts. The assay may help health care providers to monitor disease progression and antibody responses in vaccination trials, to identify health care personnel that likely are resistant to re‐infection, and recovered individuals with high antibody titers that may be suitable asplasma and/or antibody donors., We report a low‐cost FACS assay for antibodies against the native severe acute respiratory syndrome coronavirus 2 spike protein that does not require recombinant proteins or infectious virus. We demonstrate the utility of the assay by assessing antibody responses of hospitalized patients, health care personnel, and house‐holds of coronavirus diasease 2019 + individuals and their co‐quarantined room mates in the city state of Hamburg

Published
2021

28. Effective targeting of microglial P2X7 following intracerebroventricular delivery of nanobodies and nanobody-encoding AAVs

Author

Pinto-Espinoza, Carolina, primary, Guillou, Charlotte, additional, Rissiek, Björn, additional, Wilmes, Maximilian, additional, Javidi, Ehsan, additional, Schwarz, Nicole, additional, Junge, Marten, additional, Haag, Friedrich, additional, Liaukouskaya, Nastassia, additional, Wanner, Nicola, additional, Nicke, Annette, additional, Stortelers, Catelijne, additional, Tan, Yossan-Var, additional, Adriouch, Sahil, additional, Magnus, Tim, additional, and Koch-Nolte, Friedrich, additional

Published
2022
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29. Human dental pulp cells modulate CD8+ T cell proliferation and efficiently degrade extracellular ATP to adenosine in vitro

Author

Ahmadi, Parimah, primary, Yan, Ming, additional, Bauche, Andreas, additional, Smeets, Ralf, additional, Müller, Christa E., additional, Koch-Nolte, Friedrich, additional, Haag, Friedrich, additional, Fliegert, Ralf, additional, Kluwe, Lan, additional, Schulze zur Wiesch, Julian, additional, and Hartjen, Philip, additional

Published
2022
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31. 'Natural' RT6-1 and RT6-2 'Knock-Out' Mice

Author

Matthes, Martina, Hollmann, Christiane, Bertuleit, Heinrich, Kühl, Maren, Thiele, Heinz-Günter, Haag, Friedrich, Koch-Nolte, Friedrich, Haag, Friedrich, editor, and Koch-Nolte, Friedrich, editor

Published
1997
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37. Analysis of the humoral and cellular response after the third COVID ‐19 vaccination in patients with autoimmune hepatitis

Author

Hartl, Johannes, primary, Rüther, Darius Ferenc, additional, Duengelhoef, Paul Maria, additional, Brehm, Thomas Theo, additional, Steinmann, Silja, additional, Weltzsch, Jan Philipp, additional, Glaser, Fabian, additional, Sterneck, Martina, additional, Sebode, Marcial, additional, Weiler‐Normann, Christina, additional, Lütgehetmann, Marc, additional, Schaub, Golda Melina, additional, Haag, Friedrich, additional, Schramm, Christoph, additional, Wiesch, Julian Schulze zur, additional, and Lohse, Ansgar Wilhelm, additional

Published
2022
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38. Transcriptional Pattern Analysis of Virus-Specific CD8+ T Cells in Hepatitis C Infection: Increased Expression of TOX and Eomesodermin During and After Persistent Antigen Recognition

Author

Wildner, Nils H., primary, Walker, Andreas, additional, Brauneck, Franziska, additional, Ditt, Vanessa, additional, Peine, Sven, additional, Huber, Samuel, additional, Haag, Friedrich, additional, Beisel, Claudia, additional, Timm, Joerg, additional, and Schulze zur Wiesch, Julian, additional

Published
2022
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41. Inversed Ratio of CD39/CD73 Expression on γδ T Cells in HIV Versus Healthy Controls Correlates With Immune Activation and Disease Progression

Author

Kolbe, Katharina, primary, Wittner, Melanie, additional, Hartjen, Philip, additional, Hüfner, Anja-Dorothee, additional, Degen, Olaf, additional, Ackermann, Christin, additional, Cords, Leon, additional, Stellbrink, Hans-Jürgen, additional, Haag, Friedrich, additional, and Schulze zur Wiesch, Julian, additional

Published
2022
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42. SARS‐CoV‐2 vaccination response in patients with autoimmune hepatitis and autoimmune cholestatic liver disease

Author

Duengelhoef, Paul, primary, Hartl, Johannes, additional, Rüther, Darius, additional, Steinmann, Silja, additional, Brehm, Thomas T., additional, Weltzsch, Jan Philipp, additional, Glaser, Fabian, additional, Schaub, G. M., additional, Sterneck, Martina, additional, Sebode, Marcial, additional, Weiler‐Normann, Christina, additional, Addo, Marylyn M., additional, Lütgehetmann, Marc, additional, Haag, Friedrich, additional, Schramm, Christoph, additional, Schulze zur Wiesch, Julian, additional, and Lohse, Ansgar W., additional

Published
2022
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48. Analysis of the humoral and cellular response after the thirdCOVID‐19 vaccination in patients with autoimmune hepatitis.

Author

Hartl, Johannes, Rüther, Darius Ferenc, Duengelhoef, Paul Maria, Brehm, Thomas Theo, Steinmann, Silja, Weltzsch, Jan Philipp, Glaser, Fabian, Sterneck, Martina, Sebode, Marcial, Weiler‐Normann, Christina, Lütgehetmann, Marc, Schaub, Golda Melina, Haag, Friedrich, Schramm, Christoph, Wiesch, Julian Schulze zur, and Lohse, Ansgar Wilhelm

Subjects
SEROCONVERSION, AUTOIMMUNE hepatitis, CELL analysis, BOOSTER vaccines, COVID-19 vaccines, ANTIBODY titer
Abstract

Background & aims: To explore the humoral and T‐cell response to the third COVID‐19 vaccination in autoimmune hepatitis (AIH). Methods: Anti‐SARS‐CoV‐2 antibody titers were prospectively determined in 81 AIH patients and 53 healthy age‐ and sex‐matched controls >7 days (median 35) after the first COVID‐19 booster vaccination. The spike‐specific T‐cell response was assessed using an activation‐induced marker assay (AIM) in a subset of patients. Results: Median antibody levels were significantly lower in AIH compared to controls (10 908 vs. 25 000 AU/ml, p <.001), especially in AIH patients treated with MMF (N = 14, 4542 AU/ml, p =.004) or steroids (N = 27, 7326 AU/ml, p =.020). Also, 48% of AIH patients had antibody titers below the 10% percentile of the healthy controls (9194 AU/ml, p <.001). AIH patients had a high risk of failing to develop a spike‐specific T‐cell response (15/34 (44%) vs. 2/16 (12%), p =.05) and showed overall lower frequencies of spike‐specific CD4 + T cells (median: 0.074% vs 0.283; p =.01) after the booster vaccination compared to healthy individuals. In 34/81 patients, antibody titers before and after booster vaccination were available. In this subgroup, all patients but especially those without detectable/low antibodies titers (<100 AU/ml) after the second vaccination (N = 11/34) showed a strong, 148‐fold increase. Conclusion: A third COVID‐19 vaccination efficiently boosts antibody levels and T‐cell responses in AIH patients and even seroconversion in patients with the absent immune response after two vaccinations, but to a lower level compared to controls. Therefore, we suggest routinely assessing antibody levels in AIH patients and offering additional booster vaccinations to those with suboptimal responses. [ABSTRACT FROM AUTHOR]

Published
2023
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50. Prevention in neurology

Author

Doherty, Christopher, Stockmanns, Andreas, Weingärtner, Jan-Hinnerk, Job, Frank Peter, Hanrath, Peter, Ringelstein, Erich Bernd, Widder, B., Kleiser, B., Dürr, A., Hinse, Paul, Schulz, Antje, Haag, Friedrich, Carvajal, Manuel, Thie, Andreas, Grotemeyer, K.-H., Scharafiniski, H. W., Husstedt, I. W., Mielke, U., Marian, A., Lederer, K., Mayer, C., Schimrigk, K., Hartard, C., Scharein, S., Kunze, K., Rühl, H., Schimirigk, K., Strittmatter, M., Hamann, G., Reuner, C., Cramer, H., Klockgether, T., Wüllner, U., Petersen, D., Dichgans, J., Walter, G., Haaß, A., and Schimrigk, K.

Published
1992
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