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29 results on '"Hähl, Hendrik"'

3. Hydrophobin Bilayer as Water Impermeable Protein Membrane

Author

Nolle, Friederike, primary, Starke, Leonhard J., additional, Griffo, Alessandra, additional, Lienemann, Michael, additional, Jacobs, Karin, additional, Seemann, Ralf, additional, Fleury, Jean-Baptiste, additional, Hub, Jochen S., additional, and Hähl, Hendrik, additional

Published
2023
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5. Cholesterol promotes clustering of PI(4,5)P2 driving unconventional secretion of FGF2

Author

Lolicato, Fabio, primary, Saleppico, Roberto, additional, Griffo, Alessandra, additional, Meyer, Annalena, additional, Scollo, Federica, additional, Pokrandt, Bianca, additional, Müller, Hans-Michael, additional, Ewers, Helge, additional, Hähl, Hendrik, additional, Fleury, Jean-Baptiste, additional, Seemann, Ralf, additional, Hof, Martin, additional, Brügger, Britta, additional, Jacobs, Karin, additional, Vattulainen, Ilpo, additional, and Nickel, Walter, additional

Published
2022
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6. Hydroxyapatite Pellets as Versatile Model Surfaces for Systematic Adhesion Studies on Enamel: A Force Spectroscopy Case Study

Author

Mischo, Johannes, primary, Faidt, Thomas, additional, McMillan, Ryan B., additional, Dudek, Johanna, additional, Gunaratnam, Gubesh, additional, Bayenat, Pardis, additional, Holtsch, Anne, additional, Spengler, Christian, additional, Müller, Frank, additional, Hähl, Hendrik, additional, Bischoff, Markus, additional, Hannig, Matthias, additional, and Jacobs, Karin, additional

Published
2022
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7. Cholesterol promotes both head group visibility and clustering of PI(4,5)P2driving unconventional secretion of Fibroblast Growth Factor 2

Author

Lolicato, Fabio, primary, Saleppico, Roberto, additional, Griffo, Alessandra, additional, Pokrandt, Bianca, additional, Müller, Hans-Michael, additional, Ewers, Helge, additional, Hähl, Hendrik, additional, Fleury, Jean-Baptiste, additional, Seemann, Ralf, additional, Brügger, Britta, additional, Jacobs, Karin, additional, Vattulainen, Ilpo, additional, and Nickel, Walter, additional

Published
2021
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9. Hydroxyapatite pellets as versatile model surfaces for systematic studies on enamel

Author

Mischo, Johannes, primary, Faidt, Thomas, additional, McMillan, Ryan B., additional, Dudek, Johanna, additional, Gunaratnam, Gubesh, additional, Bayenat, Pardis, additional, Holtsch, Anne, additional, Spengler, Christian, additional, Müller, Frank, additional, Hähl, Hendrik, additional, Bischoff, Markus, additional, Hannig, Matthias, additional, and Jacobs, Karin, additional

Published
2021
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13. Adhesion Properties of Freestanding Hydrophobin Bilayers

Author

Hähl, Hendrik, primary, Vargas, Jose Nabor, additional, Jung, Michael, additional, Griffo, Alessandra, additional, Laaksonen, Päivi, additional, Lienemann, Michael, additional, Jacobs, Karin, additional, Seemann, Ralf, additional, and Fleury, Jean-Baptiste, additional

Published
2018
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14. Single-Molecule Force Spectroscopy Study on Modular Resilin Fusion Protein

Author

Griffo, Alessandra, primary, Hähl, Hendrik, additional, Grandthyll, Samuel, additional, Müller, Frank, additional, Paananen, Arja, additional, Ilmén, Marja, additional, Szilvay, Géza R., additional, Landowski, Christopher P., additional, Penttilä, Merja, additional, Jacobs, Karin, additional, and Laaksonen, Päivi, additional

Published
2017
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17. Synthesis of Hydroxyapatite Substrates: Bridging the Gap between Model Surfaces and Enamel

Author

Zeitz, Christian, primary, Faidt, Thomas, additional, Grandthyll, Samuel, additional, Hähl, Hendrik, additional, Thewes, Nicolas, additional, Spengler, Christian, additional, Schmauch, Jörg, additional, Deckarm, Michael Johannes, additional, Gachot, Carsten, additional, Natter, Harald, additional, Hannig, Matthias, additional, Müller, Frank, additional, and Jacobs, Karin, additional

Published
2016
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18. Dynamic Assembly of Class II Hydrophobins from T. reeseiat the Air–Water Interface

Author

Hähl, Hendrik, Griffo, Alessandra, Safaridehkohneh, Neda, Heppe, Jonas, Backes, Sebastian, Lienemann, Michael, Linder, Markus B., Santen, Ludger, Laaksonen, Päivi, and Jacobs, Karin

Abstract

Class II hydrophobins are amphiphilic proteins produced by filamentous fungi. One of their typical features is the tendency to accumulate at the interface between an aqueous phase and a hydrophobic phase, such as the air–water interface. The kinetics of the interfacial self-assembly of wild-type hydrophobins HFBI and HFBII and some of their engineered variants at the air–water interface were measured by monitoring the accumulated mass at the interface via nondestructive ellipsometry measurements. The resulting mass vs time curves revealed unusual kinetics for a monolayer formation that did not follow a typical Langmuir-type of behavior but had a rather coverage-independent rate instead. Typically, the full surface coverage was obtained at masses corresponding to a monolayer. The formation of multilayers was not observed. Atomic force microscopy revealed formation and growth of non-fusing protein clusters at the interface. The mechanism of the adsorption was studied by varying the structure or charges of the protein or the ionic strength of the subphase, revealing that the lateral interactions between the hydrophobins play a role in their interfacial assembly. Additionally, a theoretical model was introduced to identify the underlying mechanism of the unconventional adsorption kinetics.

Published
2019
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19. Self-assembled silane monolayers: A step-by-step high speed recipe for high-quality, low energy surfaces

Author

Lessel, Matthias, Bäumchen, Oliver, Klos, Mischa, Hähl, Hendrik, Fetzer, Renate, Seemann, Ralf, and Jacobs, Karin

Subjects
Condensed Matter - Materials Science, Materials Science (cond-mat.mtrl-sci), FOS: Physical sciences
Abstract

Silanization bases on the adsorption, selfassembly and covalent binding of silane molecules onto surfaces, resulting in a densely packed self-assembled monolayer (SAM). Following standard recipes, however, the quality of the monolayer is often variable and therefore unsatisfactory. The process of self-assembly is highly affected by the chemicals involved in the wet-chemical process, by the ambient parameters during preparation such as humidity or temperature and by possibly present contaminants (or impurities). Here, we present a reliable, efficient, and wet-chemical recipe for the preparation of ultra-smooth, highly ordered alkylterminated silane SAMs on Si wafers.

Published
2012
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20. Effect of Phosphate on the Molecular Properties, Interactions, and Assembly of Engineered Spider Silk Proteins

Author

Yin, Yin, Griffo, Alessandra, Gutiérrez Cruz, Adrián, Hähl, Hendrik, Jacobs, Karin, and Linder, Markus B.

Abstract

Phosphate plays a vital role in spider silk spinning and has been utilized in numerous artificial silk spinning attempts to replicate the remarkable mechanical properties of natural silk fiber. Its application in artificial processes has, however, yielded varying outcomes. It is thus necessary to investigate the origins and mechanisms behind these differences. By using recombinant silk protein SC-ADF3 derived from the garden spider Araneus diadematus, here, we describe its conformational changes under various conditions, elucidating the effect of phosphate on SC-ADF3 silk protein properties and interactions. Our results demonstrate that elevated phosphate levels induce the irreversible conformational conversion of SC-ADF3 from random coils to β-sheet structures, leading to decreased protein solubility over time. Furthermore, exposure of SC-ADF3 to phosphate stiffens already formed structures and reduces the ability to form new interactions. Our findings offer insights into the underlying mechanism through which phosphate-induced β-sheet structures in ADF3-related silk proteins impede fiber formation in the subsequent phases. From a broader perspective, our studies emphasize the significance of silk protein conformation for functional material formation, highlighting that the formation of β-sheet structures at the initial stages of protein assembly will affect the outcome of material forming processes.

Published
2024
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29. Cholesterol promotes clustering of PI(4,5)P2 driving unconventional secretion of FGF2.

Author

Lolicato, Fabio, Saleppico, Roberto, Griffo, Alessandra, Meyer, Annalena, Scollo, Federica, Pokrandt, Bianca, Müller, Hans-Michael, Ewers, Helge, Hähl, Hendrik, Fleury, Jean-Baptiste, Seemann, Ralf, Hof, Martin, Brügger, Britta, Jacobs, Karin, Vattulainen, Ilpo, and Nickel, Walter

Subjects
*CHOLESTEROL, *BILAYER lipid membranes, *CELL membranes, *MOLECULAR dynamics, *SECRETION, *PHOSPHOINOSITIDES
Abstract

FGF2 is a cell survival factor involved in tumor-induced angiogenesis that is secreted through an unconventional secretory pathway based upon direct protein translocation across the plasma membrane. Here, we demonstrate that both PI(4,5)P2-dependent FGF2 recruitment at the inner plasma membrane leaflet and FGF2 membrane translocation into the extracellular space are positively modulated by cholesterol in living cells. We further revealed cholesterol to enhance FGF2 binding to PI(4,5)P2-containing lipid bilayers. Based on extensive atomistic molecular dynamics (MD) simulations and membrane tension experiments, we proposed cholesterol to modulate FGF2 binding to PI(4,5)P2 by (i) increasing head group visibility of PI(4,5)P2 on the membrane surface, (ii) increasing avidity by cholesterol-induced clustering of PI(4,5)P2 molecules triggering FGF2 oligomerization, and (iii) increasing membrane tension facilitating the formation of lipidic membrane pores. Our findings have general implications for phosphoinositide-dependent protein recruitment to membranes and explain the highly selective targeting of FGF2 toward the plasma membrane, the subcellular site of FGF2 membrane translocation during unconventional secretion of FGF2. [ABSTRACT FROM AUTHOR]

Published
2022
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