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4 results on '"HUMAN ERYTHROVIRUSES"'

1. Extinct type of human parvovirus B19 persists in tonsillar B cells

Author

Elina Mäntylä, Lari Pyöriä, Taru Ilmarinen, Klaus Hedman, Maria Söderlund-Venermo, Leena-Maija Aaltonen, Maija Vihinen-Ranta, Mari Toppinen, Lea Hedman, Maria F. Perdomo, Medicum, Department of Virology, Clinicum, Korva-, nenä- ja kurkkutautien klinikka, Human Parvoviruses: Epidemiology, Molecular Biology and Clinical Impact, HUS Head and Neck Center, and Virus infections and immunity

Subjects
0301 basic medicine, SYNOVIAL TISSUE, viruses, Palatine Tonsil, General Physics and Astronomy, Antibodies, Viral, Genotype, INFECTION, Parvovirus B19, Human, REAL-TIME PCR, Child, Cells, Cultured, B-Lymphocytes, Multidisciplinary, biology, cell type harbouring, virus diseases, U937 Cells, Middle Aged, 3. Good health, HUMAN ERYTHROVIRUSES, solut, Real-time polymerase chain reaction, medicine.anatomical_structure, PLASMA-CELLS, Child, Preschool, GENETIC DIVERSITY, Antibody, Adult, Cell type, Adolescent, BONE-MARROW, Science, 030106 microbiology, QUANTITATIVE PCR, ta3111, Article, General Biochemistry, Genetics and Molecular Biology, Cell Line, Parvoviridae Infections, Young Adult, 03 medical and health sciences, Immune system, Cell Line, Tumor, medicine, Humans, Aged, B cells, parvovirus B19, Parvovirus, Monocyte, ta1182, General Chemistry, DNA, virus types, biology.organism_classification, Virology, CELLULAR CORECEPTOR, 030104 developmental biology, Cell culture, DNA, Viral, Immunology, biology.protein, cells, 3111 Biomedicine
Abstract

Parvovirus B19 (B19V) DNA persists lifelong in human tissues, but the cell type harbouring it remains unclear. We here explore B19V DNA distribution in B, T and monocyte cell lineages of recently excised tonsillar tissues from 77 individuals with an age range of 2–69 years. We show that B19V DNA is most frequent and abundant among B cells, and within them we find a B19V genotype that vanished from circulation >40 years ago. Since re-infection or re-activation are unlikely with this virus type, this finding supports the maintenance of pathogen-specific humoral immune responses as a consequence of B-cell long-term survival rather than continuous replenishment of the memory pool. Moreover, we demonstrate the mechanism of B19V internalization to be antibody dependent in two B-cell lines as well as in ex vivo isolated tonsillar B cells. This study provides direct evidence for a cell type accountable for B19V DNA tissue persistence., The cell type that hosts parvovirus B19 (B19V) DNA lifelong is currently unknown. Here, the authors identify tonsillar B cells as a reservoir, detect an extinct B19V type in older adults, supporting a long-term association, and show that B19V uptake into B cells is antibody dependent.

Published
2017

2. Extinct type of human parvovirus B19 persists in tonsillar B cells

Author

University of Helsinki, Medicum, University of Helsinki, Department of Virology, University of Helsinki, Korva-, nenä- ja kurkkutautien klinikka, Pyöriä, Lari, Toppinen, Mari, Mantyla, Elina, Hedman, Lea, Aaltonen, Leena-Maija, Vihinen-Ranta, Maija, Ilmarinen, Taru, Soderlund-Venermo, Maria, Hedman, Klaus, Perdomo, Maria, University of Helsinki, Medicum, University of Helsinki, Department of Virology, University of Helsinki, Korva-, nenä- ja kurkkutautien klinikka, Pyöriä, Lari, Toppinen, Mari, Mantyla, Elina, Hedman, Lea, Aaltonen, Leena-Maija, Vihinen-Ranta, Maija, Ilmarinen, Taru, Soderlund-Venermo, Maria, Hedman, Klaus, and Perdomo, Maria

Abstract

Parvovirus B19 (B19V) DNA persists lifelong in human tissues, but the cell type harbouring it remains unclear. We here explore B19V DNA distribution in B, T and monocyte cell lineages of recently excised tonsillar tissues from 77 individuals with an age range of 2-69 years. We show that B19V DNA is most frequent and abundant among B cells, and within them we find a B19V genotype that vanished from circulation 440 years ago. Since re-infection or re-activation are unlikely with this virus type, this finding supports the maintenance of pathogen-specific humoral immune responses as a consequence of B-cell long-term survival rather than continuous replenishment of the memory pool. Moreover, we demonstrate the mechanism of B19V internalization to be antibody dependent in two B-cell lines as well as in ex vivo isolated tonsillar B cells. This study provides direct evidence for a cell type accountable for B19V DNA tissue persistence.

Published
2017

3. Bones hold the key to DNA virus history and epidemiology

Author

Samantha Lycett, Klaus Hedman, Mari Toppinen, Maria F. Perdomo, Antti Sajantila, Jorma Palo, Peter Simmonds, Maria Söderlund-Venermo, Department of Virology, Medicum, Forensic Medicine, Klaus Hedman / Principal Investigator, Human Parvoviruses: Epidemiology, Molecular Biology and Clinical Impact, Virus infections and immunity, and PaleOmics Laboratory

Subjects
Most recent common ancestor, HUMAN PARVOVIRUS B19, Genotype, World War II, MARROW CELL-CULTURES, Exhumation, Biology, Real-Time Polymerase Chain Reaction, Article, Bone and Bones, VIRAL EVOLUTION, Parvoviridae Infections, EMERGENCE, 03 medical and health sciences, Virology, INFECTION, Cadaver, Parvovirus B19, Human, Prevalence, ANCIENT DNA, Humans, PHYLOGENETIC ANALYSIS, Phylogeny, 030304 developmental biology, Genetics, 0303 health sciences, Multidisciplinary, IDENTIFICATION, 030306 microbiology, Viral Epidemiology, Parvovirus, PERSISTENCE, Haplotype, DNA virus, History, 20th Century, biology.organism_classification, HUMAN ERYTHROVIRUSES, Europe, Military Personnel, Ancient DNA, TISSUE, Viral evolution, DNA, Viral, REPLICATION, 3111 Biomedicine, USSR
Abstract

DNA in human skeletal remains represents an important historical source of host genomic information and potentially of infecting viruses. However, little is known about viral persistence in bone. We searched ca. 70-year-old long bones of putative Finnish casualties from World War II for parvovirus B19 (B19V) DNA and found a remarkable prevalence of 45%. The viral sequences were exclusively of genotypes 2 (n = 41), which disappeared from circulation in 1970´s, or genotype 3 (n = 2), which has never been reported in Northern Europe. Based on mitochondrial and Y-chromosome profiling, the two individuals carrying B19V genotype 3 were likely from the Soviet Red Army. The most recent common ancestor for all genotypes was estimated at early 1800s. This work demonstrates the forms of B19V that circulated in the first half of the 20th century and provides the first evidence of the suitability of bone for exploration of DNA viruses.

Published
2015
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4. Bones hold the key to DNA virus history and epidemiology

Author

University of Helsinki, Medicum, Toppinen, M., Perdomo, Maria, Palo, J. U., Simmonds, P., Lycett, S. J., Soderlund-Venermo, M., Sajantila, A., Hedman, K., University of Helsinki, Medicum, Toppinen, M., Perdomo, Maria, Palo, J. U., Simmonds, P., Lycett, S. J., Soderlund-Venermo, M., Sajantila, A., and Hedman, K.

Abstract

DNA in human skeletal remains represents an important historical source of host genomic information and potentially of infecting viruses. However, little is known about viral persistence in bone. We searched ca. 70-year-old long bones of putative Finnish casualties from World War II for parvovirus B19 (B19V) DNA, and found a remarkable prevalence of 45%. The viral sequences were exclusively of genotypes 2 (n = 41), which disappeared from circulation in 1970's, or genotype 3 (n = 2), which has never been reported in Northern Europe. Based on mitochondrial and Y-chromosome profiling, the two individuals carrying B19V genotype 3 were likely from the Soviet Red Army. The most recent common ancestor for all genotypes was estimated at early 1800s. This work demonstrates the forms of B19V that circulated in the first half of the 20th century and provides the first evidence of the suitability of bone for exploration of DNA viruses.

Published
2015

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