Wang,Ziyan, Liu,Chengxin, Wei,Jiaming, Yuan,Hui, Shi,Min, Zhang,Fei, Zeng,Qinghua, Huang,Aisi, Du,Lixin, Li,Ya, Guo,Zhihua, Wang,Ziyan, Liu,Chengxin, Wei,Jiaming, Yuan,Hui, Shi,Min, Zhang,Fei, Zeng,Qinghua, Huang,Aisi, Du,Lixin, Li,Ya, and Guo,Zhihua
Ziyan Wang,1,2,* Chengxin Liu,1,2,* Jiaming Wei,2,3 Hui Yuan,1,2 Min Shi,2,3 Fei Zhang,2,3 Qinghua Zeng,2,3 Aisi Huang,2,3 Lixin Du,4 Ya Li,4 Zhihua Guo2,3 1First Clinical College of Chinese Medicine, Hunan University of Chinese Medicine, Changsha, 410208, Peopleâs Republic of China; 2Hunan Key Laboratory of Colleges and Universities of Intelligent Traditional Chinese Medicine Diagnosis and Preventive Treatment of Chronic Diseases of Hunan Universities of Chinese Medicine, Hunan University of Chinese Medicine, Changsha, 410208, Peopleâs Republic of China; 3School of Traditional Chinese Medicine, Hunan University of Chinese Medicine, Changsha, 410208, Peopleâs Republic of China; 4School of Pharmacy, Hunan University of Chinese Medicine, Changsha, 410208, Peopleâs Republic of China*These authors contributed equally to this workCorrespondence: Ya Li; Zhihua Guo, Email 003872@hnucm.edu.cn; 004294@hnucm.edu.cnObjective: This study aims to explore the mechanism of action of Yixintai in treating chronic ischemic heart failure by combining bioinformatics and experimental validation.Materials and Methods: Five potential drugs for treating heart failure were obtained from Yixintai (YXT) through early mass spectrometry detection. The targets of YXT for treating heart failure were obtained by a search of online databases. Gene ontology (GO) functional enrichment analysis and Kyoto encyclopedia of genes and genomes (KEGG) pathway enrichment analyses were conducted on the common targets using the DAVID database. A rat heart failure model was established by ligating the anterior descending branch of the left coronary artery. A small animal color Doppler ultrasound imaging system detected cardiac function indicators. Hematoxylin-eosin (HE), Massonâs, and electron microscopy were used to observe the pathological morphology of the myocardium in rats with heart failure. The network pharmacology analysis results were validated by ELISA, qPCR, and Weste