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524 results on '"HOUGHTEN, R. A."'

32. A CTL epitope on the circumsporozoite protein of P. yoelii

Author

Weiss, W. R., Houghten, R. A., Good, M. F., Berzofsky, J. A., Miller, L. H., and Hoffman, S. L.

Subjects
Mice, Inbred BALB C, Molecular Sequence Data, Protozoan Proteins, Antigens, Protozoan, Plasmodium yoelii, Epitopes, Mice, parasitic diseases, Animals, Amino Acid Sequence, Cells, Cultured, Spleen, Research Article, T-Lymphocytes, Cytotoxic
Abstract

Humans are infected with malaria by the bite of anophelene mosquitos carrying plasmodia sporozoites. These sporozoites pass quickly from the blood into hepatocytes, where they develop into mature liver-stage parasites over several days. The clinical stage of the illness begins only when the liver-stage parasites rupture into the bloodstream and erythrocytes are invaded. The pre-erythrocytic stages of malaria are inviting targets for vaccine development, because an effective immune response to these early stages would prevent symptomatic infections.

Published
1990

34. Identification of a nonameric H-2Kk-restricted CD8+ cytotoxic T lymphocyte epitope on the Plasmodium falciparum circumsporozoite protein

Author

Malik, A, Houghten, R, Corradin, G, Buus, S, Berzofsky, J A, Hoffman, S L, Malik, A, Houghten, R, Corradin, G, Buus, S, Berzofsky, J A, and Hoffman, S L

Abstract

Udgivelsesdato: 1995-May, Class I-restricted CD8+ cytotoxic T lymphocytes (CTL) against the circumsporozoite protein (CSP) protect mice against the rodent malaria parasite, Plasmodium yoelii, and vaccines designed to produce protective CTL against the P. falciparum CSP (PfCSP) are under development. Humans and B10.BR (H-2k) mice have been shown to have CD8+ CTL activity against a 23-amino-acid region of the PfCSP (residues 368 to 390 from the PfCSP 7G8 sequence) that is too long to bind directly to class I major histocompatibility complex molecules. To identify within this 23-amino-acid peptide a shorter peptide that binds to an H-2k class I major histocompatibility molecule, a primarily CD8+ (97.8%) T-cell line (PfCSP TCL.1) was produced by immunizing B10.BR mice with recombinant vaccinia virus expressing the PfCSP and stimulating in vitro spleen cells from these immunized mice with L cells transfected with the PfCSP gene (LPF cells). PfCSP TCL.1 lysed LPF cells and L cells pulsed with peptide PfCSP 7G8 368-390. When 15 overlapping nonamer peptides spanning the 368 to 390 sequence were tested, only one peptide, PfCSP 7G8 375-383 (Y E N D I E K K I), which includes an H-2Kk-binding motif, E at amino acid residue 2, and I at residue 9, sensitized targets for lysis by PfCSP TCL.1. Furthermore, a 10(3)- to 10(4)-fold lower concentration of the nonamer than that of the 23-amino-acid peptide was required to sensitize target cells for lysis by PfCSP TCL.1. Presentation by H-2Kk was demonstrated by using 3T3 fibroblast cells transfected with the murine H-2Kk or H-2Dk genes, and only the H-2Kk transfectants were lysed by PfCSP TCL.1 after incubation with peptide PfCSP 7G8 375-383. Binding to H-2Kk was confirmed by competitive inhibition of binding of labelled peptides to affinity-purified Kk molecules. Substitution of the anchor amino acid residue, E, at position 2 with A dramatically reduced binding to Kk and eliminated the capacity of the peptide to sensitize target cells for killing. Variation of

Published
1995

35. ChemInform Abstract: Manual Multiple Synthesis Methods

Author

Dorner, B., primary, Ostresh, J. M., additional, Houghten, R. A., additional, Frank, Ronald, additional, Tiepold, Andrea, additional, Fox, John E., additional, Bray, Andrew M., additional, Ede, Nicholas J., additional, James, Ian W., additional, and Wickham, Geoffrey, additional

Published
2000
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38. A CTL Epitope on the Circumsporozoite Protein of P. Yoelii

Author

NAVAL MEDICAL RESEARCH INST BETHESDA MD, Weiss, W. R., Houghten, R. A., Good, M. F., Berzofsky, J. A., Miller, L. H., NAVAL MEDICAL RESEARCH INST BETHESDA MD, Weiss, W. R., Houghten, R. A., Good, M. F., Berzofsky, J. A., and Miller, L. H.

Abstract

A vaccine model exists for protection against the preerythrocytic stages of malaria. Humans and rodents can be protected against sporozoite infection by immunization with radiation-attenuated sporozoites. There is evidence from different animal models that both antibodies and T cells may be involved in this immune response. However, when BALB/c mice are immunized with Plasmodium yoelii sporozoites, it appears that antibody alone plays a minor protective role, while CD8+T cells have critically important anti-sporozoite functions. The parasite antigens recognized by these protective CD8+ T cells have not been identified., Published in Bulletin of the World Health Organization, v68 (Suppl.) p99-103, 1990.

Published
1990

39. Development of two monoclonal antibodies against Plasmodium falciparum sporozoite surface protein 2 and mapping of B-cell epitopes

Author

Charoenvit, Y, primary, Fallarme, V, additional, Rogers, W O, additional, Sacci, J B, additional, Kaur, M, additional, Aguiar, J C, additional, Yuan, L F, additional, Corradin, G, additional, Andersen, E, additional, Wizel, B, additional, Houghten, R A, additional, Oloo, A, additional, De la Vega, P, additional, and Hoffman, S L, additional

Published
1997
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