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The Programmable Hyperspectral Seawater Scanner (PHySS) represents a significant breakthrough in monitoring harmful algal blooms (HABs), specifically targeting the "Florida red tide" caused by Karenia brevis. By utilizing a Fourth-Derivative Spectral Similarity Index (SI), this study establishes a strong positive correlation between the SI and phytoplankton counts, underscoring the PHySS's potential for early detection and effective management of HABs. Our findings suggest that the PHySS could act as a predictive tool, offering crucial lead time to mitigate the ecological and economic repercussions of blooms. However, this study also identifies certain limitations of the PHySS technology, such as its inability to differentiate among various phytoplankton species without additional physical verification of cell counts. This limitation highlights the need for a multifaceted approach to HAB management. Our research suggests that adopting a multi-modal monitoring strategy could lead to more sophisticated and effective methods for combating HABs, fostering an optimistic outlook for future advancements in this area. [ABSTRACT FROM AUTHOR]

The Programmable Hyperspectral Seawater Scanner (PHySS) represents a significant breakthrough in monitoring harmful algal blooms (HABs), specifically targeting the “Florida red tide” caused by Karenia brevis. By utilizing a Fourth-Derivative Spectral Similarity Index (SI), this study establishes a strong positive correlation between the SI and phytoplankton counts, underscoring the PHySS’s potential for early detection and effective management of HABs. Our findings suggest that the PHySS could act as a predictive tool, offering crucial lead time to mitigate the ecological and economic repercussions of blooms. However, this study also identifies certain limitations of the PHySS technology, such as its inability to differentiate among various phytoplankton species without additional physical verification of cell counts. This limitation highlights the need for a multifaceted approach to HAB management. Our research suggests that adopting a multi-modal monitoring strategy could lead to more sophisticated and effective methods for combating HABs, fostering an optimistic outlook for future advancements in this area.

A variety of microalgal species produce lipophilic toxins (LT) that are accumulated by filter-feeding bivalves. Their negative impacts on human health and shellfish exploitation are determined by toxic potential of the local strains and toxin biotransformations by exploited bivalve species. Chile has become, in a decade, the world’s major exporter of mussels (Mytilus chilensis) and scallops (Argopecten purpuratus) and has implemented toxin testing according to importing countries’ demands. Species of the Dinophysis acuminata complex and Protoceratium reticulatum are the most widespread and abundant LT producers in Chile. Dominant D. acuminata strains, notwithstanding, unlike most strains in Europe rich in okadaic acid (OA), produce only pectenotoxins, with no impact on human health. Dinophysis acuta, suspected to be the main cause of diarrhetic shellfish poisoning outbreaks, is found in the two southernmost regions of Chile, and has apparently shifted poleward. Mouse bioassay (MBA) is the official method to control shellfish safety for the national market. Positive results from mouse tests to mixtures of toxins and other compounds only toxic by intraperitoneal injection, including already deregulated toxins (PTXs), force unnecessary harvesting bans, and hinder progress in the identification of emerging toxins. Here, 50 years of LST events in Chile, and current knowledge of their sources, accumulation and effects, are reviewed. Improvements of monitoring practices are suggested, and strategies to face new challenges and answer the main questions are proposed.

Importance: Harmful algal blooms are among the most significant threats to drinking water safety. Blooms dominated by cyanobacteria can produce potentially harmful toxins and, despite intensive research, toxin production remains unpredictable. We measured gaseous molecules in Upper Klamath Lake, Oregon, over 2 years and used them to predict the presence and concentration of the cyanotoxin, microcystin, and microbial community composition. Subsets of gaseous compounds were identified that are associated with microcystin production during oxidative stress, pointing to ecosystem-level interactions leading to microcystin contamination. Our approach shows potential for gaseous molecules to be harnessed in monitoring critical waterways., Competing Interests: The authors declare no conflict of interest.

From June 2006 to January 2007 passive samplers (solid phase adsorbing toxin tracking, SPATT) were tested as a monitoring tool with weekly monitoring of phytoplankton and toxin content (liquid chromatography–mass spectrometry, LC-MS) in picked cells of Dinophysis and plankton concentrates. Successive blooms of Dinophysis acuminata, D. acuta and D. caudata in 2006 caused a long mussel harvesting closure (4.5 months) in the Galician Rías (NW Spain) and a record (up to 9246 ng·g resin-week−1) accumulation of toxins in SPATT discs. Best fit of a toxin accumulation model was between toxin accumulation in SPATT and the product of cell densities by a constant value, for each species of Dinophysis, of toxin content (average) in picked cells. Detection of Dinophysis populations provided earlier warning of oncoming diarrhetic shellfish poisoning (DSP) outbreaks than the SPATT, which at times overestimated the expected toxin levels in shellfish because: (i) SPATT accumulated toxins did not include biotransformation and depuration loss terms and (ii) accumulation of toxins not available to mussels continued for weeks after Dinophysis cells were undetectable and mussels were toxin-free. SPATT may be a valuable environmental monitoring and research tool for toxin dynamics, in particular in areas with no aquaculture, but does not provide a practical gain for early warning of DSP outbreaks.

Diarrhetic shellfish toxins produced by the dinoflagellate genus Dinophysis are a major problem for the shellfish industry worldwide. Separate species of the genus have been associated with the production of different analogues of the okadaic acid group of toxins. To evaluate the spatial and temporal variability of Dinophysis species and toxins in the important shellfish-harvesting region of the Scottish west coast, we analysed data collected from 1996 to 2017 in two contrasting locations: Loch Ewe and the Clyde Sea. Seasonal studies were also undertaken, in Loch Ewe in both 2001 and 2002, and in the Clyde in 2015. Dinophysis acuminata was present throughout the growing season during every year of the study, with blooms typically occurring between May and September at both locations. The appearance of D. acuta was interannually sporadic and, when present, was most abundant in the late summer and autumn. The Clyde field study in 2015 indicated the importance of a temperature front in the formation of a D. acuta bloom. A shift in toxin profiles of common mussels (Mytilus edulis) tested during regulatory monitoring was evident, with a proportional decrease in okadaic acid (OA) and dinophysistoxin-1 (DTX1) and an increase in dinophysistoxin-2 (DTX2) occurring when D. acuta became dominant. Routine enumeration of Dinophysis to species level could provide early warning of potential contamination of shellfish with DTX2 and thus determine the choice of the most suitable kit for effective end-product testing.

From June 2006 to January 2007 passive samplers (solid phase adsorbing toxin tracking, SPATT) were tested as a monitoring tool with weekly monitoring of phytoplankton and toxin content (liquid chromatography-mass spectrometry, LC-MS) in picked cells of Dinophysis and plankton concentrates. Successive blooms of Dinophysis acuminata, D. acuta and D. caudata in 2006 caused a long mussel harvesting closure (4.5 months) in the Galician Rías (NW Spain) and a record (up to 9246 ng·g resin-week-1) accumulation of toxins in SPATT discs. Best fit of a toxin accumulation model was between toxin accumulation in SPATT and the product of cell densities by a constant value, for each species of Dinophysis, of toxin content (average) in picked cells. Detection of Dinophysis populations provided earlier warning of oncoming diarrhetic shellfish poisoning (DSP) outbreaks than the SPATT, which at times overestimated the expected toxin levels in shellfish because: (i) SPATT accumulated toxins did not include biotransformation and depuration loss terms and (ii) accumulation of toxins not available to mussels continued for weeks after Dinophysis cells were undetectable and mussels were toxin-free. SPATT may be a valuable environmental monitoring and research tool for toxin dynamics, in particular in areas with no aquaculture, but does not provide a practical gain for early warning of DSP outbreaks. [ABSTRACT FROM AUTHOR]

Fluorescence in situ hybridisation (FISH) is a powerful molecular biological tool to detect and enumerate harmful microorganism in the marine environment. Different FISH methods are available, and especially in combination with automated counting techniques, the potential for a routine monitoring of harmful marine microalgae is attainable. Various oligonucleotide probes are developed for detecting harmful microalgae. However, FISH-based methods are not yet regularly included in monitoring programmes tracking the presence of harmful marine microalgae. A limitation factor of the FISH technique is the currently available number of suited fluorochromes attached to the FISH probes to detect various harmful species in one environmental sample at a time. However, coupled automated techniques, like flow cytometry or solid-phase cytometry, can facilitate the analysis of numerous field samples and help to overcome this drawback. A great benefit of FISH in contrast to other molecular biological detection methods for harmful marine microalgae is the direct visualisation of the hybridised target cells, which are not permitted in cell free formats, like DNA depending analysis methods. Therefore, an additional validation of the FISH-generated results is simultaneously given. [ABSTRACT FROM AUTHOR]

Abstract: The effects of the toxic dinoflagellate Heterocapsa circularisquama on the valve movements of adult Ruditapes philippinarum (Bivalvia, Veneridae) were investigated. The valve movement behaviour was continuously measured using a non invasive Hall element sensor-automated data acquisition system. Individual clams acclimated to the experimental conditions were subjected to different food regimes over a 96 h period: (1) unfed in filtered seawater for the first 24 h, (2) batch-fed1, in which clams were given one ration of non-toxic Isochrysis galbana at the beginning of the second 24 h, (3) batch-fed2, in which clams were given a mixture of I. galbana and H. circularisquama (5×104 cells ml−1) for the third 24 h, and (4) starved for 24 h in filtered seawater after exposure to the toxic alga. For each food regime, the valve activity was analyzed by quantifying: (1) the duration of valve opening (DVO, %), (2) the amplitude of valve opening (AVO, mm), and (3) the frequency of valve adductions (FVA, adductions h−1). The real-time monitoring of the clams'' valve movements proved that R. philippinarum detects H. circularisquama in the mixture of food at as low as 0.5 cells ml−1. Typically, the addition of the toxic alga induced a short and incomplete valve closure reaction. A strong correlation between the closure reaction time and H. circularisquama concentration was found (R 2 =0.91), the closure reaction occurring faster with increasing concentrations. Reopening of the valves was characterized by a significant increase in the FVA starting from 5 cells ml−1 (ANOVA, P <0.001) and a significant decrease in the AVO at 500 cells ml−1 (Wilcoxon, P <0.05). After exposure, clams transferred to clean seawater resumed normal valve adduction activity, but showed a significant increase in the DVO after exposure to 0.5 and 5 cells ml−1 (ANOVA, LSD, P <0.05), and a significant increase in the AVO (ANOVA, P <0.01). Such increase could have been associated with recovery behaviour. Our results indicate that the Manila clam is highly sensitive to the toxic H. circularisquama and detects efficiently its presence at extremely low concentrations, in mixture of food, and in a time- and concentration-dependent manner. The Manila clam and the valve movement monitor could thus be used as an early biological warning system to monitor the outbreaks of this toxic alga along marine coastal areas and in bivalve farms. However, field experiments are required to provide further evidence for the use of this monitor in natural environment where several factors may interfere with the valve movement behaviour. [Copyright &y& Elsevier]

A variety of microalgal species produce lipophilic toxins (LT) that are accumulated by filter-feeding bivalves. Their negative impacts on human health and shellfish exploitation are determined by toxic potential of the local strains and toxin biotransformations by exploited bivalve species. Chile has become, in a decade, the world's major exporter of mussels (Mytilus chilensis) and scallops (Argopecten purpuratus) and has implemented toxin testing according to importing countries' demands. Species of the Dinophysis acuminata complex and Protoceratium reticulatum are the most widespread and abundant LT producers in Chile. Dominant D. acuminata strains, notwithstanding, unlike most strains in Europe rich in okadaic acid (OA), produce only pectenotoxins, with no impact on human health. Dinophysis acuta, suspected to be the main cause of diarrhetic shellfish poisoning outbreaks, is found in the two southernmost regions of Chile, and has apparently shifted poleward. Mouse bioassay (MBA) is the official method to control shellfish safety for the national market. Positive results from mouse tests to mixtures of toxins and other compounds only toxic by intraperitoneal injection, including already deregulated toxins (PTXs), force unnecessary harvesting bans, and hinder progress in the identification of emerging toxins. Here, 50 years of LST events in Chile, and current knowledge of their sources, accumulation and effects, are reviewed. Improvements of monitoring practices are suggested, and strategies to face new challenges and answer the main questions are proposed. [ABSTRACT FROM AUTHOR]

Harmful algae blooms (HABs) cause acute effects on marine ecosystems due to their production of endogenous toxins or their enormous biomass, leading to significant impacts on local economies and public health. Although HAB monitoring has been intensively performed at spatiotemporal scales in coastal areas of the world over the last decades, procedures have not yet been standardized. HAB monitoring procedures are complicated and consist of many methodologies, including physical, chemical, and biological water sample measurements. Each monitoring program currently uses different combinations of methodologies depending on site specific purposes, and many prior programs refer to the procedures in quotations. HAB monitoring programs in Chile have adopted the traditional microscopic and toxin analyses but not molecular biology and bacterial assemblage approaches. Here we select and optimize the HAB monitoring methodologies suitable for Chilean geography, emphasizing on metabarcoding analyses accompanied by the classical tools with considerations including cost, materials and instrument availability, and easiness and efficiency of performance. We present results from a pilot study using the standardized stepwise protocols, demonstrating feasibility and plausibility for sampling and analysis for the HAB monitoring. Such specific instructions in the standardized protocol are critical obtaining quality data under various research environments involving multiple stations, different analysts, various time-points, and long HAB monitoring duration.

Diarrhetic shellfish toxins produced by the dinoflagellate genus Dinophysis are a major problem for the shellfish industry worldwide. Separate species of the genus have been associated with the production of different analogues of the okadaic acid group of toxins. To evaluate the spatial and temporal variability of Dinophysis species and toxins in the important shellfish-harvesting region of the Scottish west coast, we analysed data collected from 1996 to 2017 in two contrasting locations: Loch Ewe and the Clyde Sea. Seasonal studies were also undertaken, in Loch Ewe in both 2001 and 2002, and in the Clyde in 2015. Dinophysis acuminata was present throughout the growing season during every year of the study, with blooms typically occurring between May and September at both locations. The appearance of D. acuta was interannually sporadic and, when present, was most abundant in the late summer and autumn. The Clyde field study in 2015 indicated the importance of a temperature front in the formation of a D. acuta bloom. A shift in toxin profiles of common mussels (Mytilus edulis) tested during regulatory monitoring was evident, with a proportional decrease in okadaic acid (OA) and dinophysistoxin-1 (DTX1) and an increase in dinophysistoxin-2 (DTX2) occurring when D. acuta became dominant. Routine enumeration of Dinophysis to species level could provide early warning of potential contamination of shellfish with DTX2 and thus determine the choice of the most suitable kit for effective end-product testing. [ABSTRACT FROM AUTHOR]