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This paper introduces a method for improving parameter estimation in statistical models. Parameter estimation is a popular area of study in statistics, and recent years have seen the introduction of new distributions with more parameters to enhance modelling success. While finding a suitable model for a dataset is crucial, accurately estimating parameters is equally important. In some cases, classical parameter estimation methods fail to provide a closed form of estimation for parameters. As a result, researchers commonly resort to numerical methods and software programs for parameter estimation in models. The success rates of models have gained significance with the rising popularity of novel techniques like machine learning algorithms and artificial neural networks. Robust and reliable models are built on the strong theoretical foundations of statistical distributions. Specific distributions are used in various research fields to model datasets, and the assumptions associated with these distributions provide valuable insights into observations. Additionally, parameter estimation results sometimes lead researchers to direct conclusions. This paper presents an improvement method that relies on the estimation of parameters from other statistical distributions. This novel approach aims to make parameter estimation easier and more successful in certain situations. In the applications in this paper, the proposed methodology improves the success rate by up to 10% which provides an additional 6% success in the models.

In this study we aim to examine genotypic structures in terms of the κ-casein (CSN3) gene locus in 70 head of Simmental cattle raised in a private enterprise in Erzurum to determine the distribution of genotypes and allele frequencies in cattle in terms of related genes and to correlate the determined genotypes with some performance characteristics. CSN3/HinfI gene polymorphisms were identified in DNA isolated from blood samples taken from Simmental cattle used in the study using the polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) method. The frequency of the AA, AB, and BB genotype of the CSN3 gene in the population was 57.14 %, 32.86 %, and 10.00 %, respectively; the frequency of the A allele was 0.74; and the frequency of the B allele was 0.26. The mean value for the AA, AB, and BB genotypes was 5151 ± 308.6, 5805 ± 370.3, and 5772 ± 547.3 kg for real milk yield, respectively; 5313 ± 233.9, 5784 ± 280.7, and 6458 ± 414.8 kg in 305 d for milk yield; 17.9 ± 0.75, 18.6 ± 0.89, and 19.6 ± 1.32 kg for daily milk yield; and 294 ± 13.7, 316 ± 16.5, and 294 ± 24.4 d during the lactation period. The distribution of the CSN3 gene locus in the studied population is in genetic equilibrium according to the Hardy–Weinberg principle. The genotype and allele frequencies determined in terms of CSN3 gene polymorphism can be considered sufficient to reveal the genotype diversity of the breed, and it was determined that the relationship of CSN3 genotypes was only significant with 305 d milk yield (P) in terms of the association between CSN3 genotypes and some performance traits. It has been concluded that animals with the CSN3 BB genotype have an economic advantage within the herd and that CSN3 can be used for marker-assisted selection (MAS) in this regard.

Low-cost sensors offer an attractive solution to the challenge of establishing affordable and dense spatio-temporal air quality monitoring networks with greater mobility and lower maintenance costs. These low-cost sensors offer reasonably consistent measurements but require in-field calibration to improve agreement with regulatory instruments. In this paper, we report the results of a deployment and calibration study on a network of six air quality monitoring devices built using the Alphasense O3 (OX-B431) and NO2 (NO2-B43F) electrochemical gas sensors. The sensors were deployed in two phases over a period of 3 months at sites situated within two megacities with diverse geographical, meteorological and air quality parameters. A unique feature of our deployment is a swap-out experiment wherein three of these sensors were relocated to different sites in the two phases. This gives us a unique opportunity to study the effect of seasonal, as well as geographical, variations on calibration performance. We report an extensive study of more than a dozen parametric and non-parametric calibration algorithms. We propose a novel local non-parametric calibration algorithm based on metric learning that offers, across deployment sites and phases, an R2 coefficient of up to 0.923 with respect to reference values for O3 calibration and up to 0.819 for NO2 calibration. This represents a 4–20 percentage point increase in terms of R2 values offered by classical non-parametric methods. We also offer a critical analysis of the effect of various data preparation and model design choices on calibration performance. The key recommendations emerging out of this study include (1) incorporating ambient relative humidity and temperature into calibration models; (2) assessing the relative importance of various features with respect to the calibration task at hand, by using an appropriate feature-weighing or metric-learning technique; (3) using local calibration techniques such as k nearest neighbors (KNN); (4) performing temporal smoothing over raw time series data but being careful not to do so too aggressively; and (5) making all efforts to ensure that data with enough diversity are demonstrated in the calibration algorithm while training to ensure good generalization. These results offer insights into the strengths and limitations of these sensors and offer an encouraging opportunity to use them to supplement and densify compliance regulatory monitoring networks.

Soil compaction is a common problem of mineral soils under conventional tillage practices. Organic matter addition is an efficient way of reducing the effects of field traffic in soil compaction. The aim of this study was to investigate the effects of number of tractor passes (one, three, and five) on depth-dependent (0–10 and 10–20 cm) penetration resistance, bulk density, and porosity of clay-textured soil (Typic Xerofluvent) under organic vegetable cultivation practices in the 2010–2013 growing seasons. Fields were treated with farmyard manure (FYM, 35 t ha−1), green manure (GM; common vetch, Vicia sativa L.), and conventional tillage (CT). The number of tractor passes resulted in increases in bulk density and penetration resistance (CT > GM > FYM), whereas the volume of total and macropores decreased. The maximum penetration resistance (3.60 MPa) was recorded in the CT treatment with five passes at 0–10 cm depth, whereas the minimum (1.64 MPa) was observed for the FYM treatment with one pass at 10–20 cm depth. The highest bulk density was determined as 1.61 g cm−3 for the CT treatment with five passes at 10–20 cm depth; the smallest value was 1.25 g cm−3 in the FYM treatment with only one pass at 0–10 cm depth. The highest total and macropore volumes were determined as 0.53 and 0.16 cm3 cm−3 respectively at 0–10 cm depth for the FYM treatment with one pass. The volume of micropores (0.38 cm3 cm−3) was higher at 0–10 cm depth for the FYM treatment with three passes. It can be concluded that organic pre-composted organic amendment rather than green manure is likely to be more efficient in mitigating compaction problems in soil.

In this study, the effects of a diet including fenugreek (Trigonella foenum-graecum) seed on feed consumption and milk performance were examined. A total of 24 buffaloes were randomly divided into two groups (12 animals each). The diet containing ground fenugreek seed (GFS) improved total dry matter (13.17–14.00 kg day−1) (P −1) (P −1) (P

Phosphorus (P) fertilization is commonly based on soil testing, for which a variety of different soil P extraction methods are in use. The aim of this study was to compare 14 soil P extraction methods in terms of their extraction yield and their relation to soil properties. Fifty contrasting agricultural topsoils were sampled from Austria and Germany. The soils were extracted with the following methods/extractants: H2O, CaCl2, LiCl, Olsen, Bray and Kurtz II (Bray II), Mehlich 3, calcium-acetate-lactate (CAL), iron oxide impregnated filter papers (Fe-oxide Pi), cation and anion exchange membranes (CAEM), acid ammonium oxalate, citrate-bicarbonate-dithionite, HCl, organic P and total P. The extracted P varied over three orders of magnitude and increased in the order H2O < CaCl2 < LiCl < Fe-oxide Pi < Olsen < CAL < CAEM < Mehlich 3 < Bray II < dithionite < organic P < HCl < oxalate < total P. This sequence is in accordance with previous studies and reflects different extraction mechanisms and P pools. The different extraction methods were generally well correlated, especially when P extraction was achieved by a similar mechanism. The soil properties most influential on P extractability were pH, carbonate content, texture as well as iron oxide content and crystallinity. Our results show that the different extraction methods extract distinct pools of soil P with strongly varying extractability, and that the extractability of a given pool may be influenced by different soil properties to different extents. If and how these relationships translate to plant P uptake requires further examination.

The department for Planetary Geodesy at Technical University Berlin is developing routines for photogrammetric processing of planetary image data to derive 3D representations of planetary surfaces. The Integrated Software for Imagers and Spectrometers (ISIS) software (Anderson et al., 2004), developed by USGS, Flagstaff, is readily available, open source, and very well documented. Hence, ISIS was chosen as a prime processing platform and tool kit. However, ISIS does not provide a full photogrammetric stereo processing chain. Several components like image matching, bundle block adjustment (until recently) or digital terrain model (DTM) interpolation from 3D object points are missing. Our group aims to complete this photogrammetric stereo processing chain by implementing the missing components, taking advantage of already existing ISIS classes and functionality. We report here on the current status of the development of our stereo processing chain and its first application on the Lunar Apollo landing sites.

Introduction Selling of tobacco products to minors has been banned since 1996 by the tobacco control law in Turkey. However, it is also important for the public to support practices that prevent the access of tobacco products to minors. In addition, every individual has the responsibility of carrying out society based programs that restrict access to tobacco products especially to children and the youths. Social sensitivity is considered an important factor in the prevention of tobacco use. This study aims to learn about the opinions and attitudes of adults with regards to minors access to tobacco products. Material and Methods The study was a descriptive study conducted in nine city centers in Turkey. The total number of participants reached was 3241. The questionnaire was developed by the research team and consisted of 22 questions concerning knowledge and behaviors of adults on restriction of tobacco sales to minors and their observations with regards tobacco sales to minors. Data was collected through face to face interview. Pearson chi-square test was used for the bivariate analysis whereas logistic regression was investigate the relationship between “the participant’s response against tobacco sales to minors” and the following explanatory variables; “age”, “educational status”, “income level”, “working status”, “minors access to cigarettes”, “smoking ratio in high school” and “sales of tobacco to minors”. Results More than half of the participants (60.5%) belonged to the age group 25–44 years, 61.3% graduated from high school or university. Most of the participants were smoker (39.2%) or ex-smoker (19.1%), and 41.7% of the participants was non-smoker. A greater proportion of the participants (76.2%) believed that smoking prevalence was greater than 40% among high school students. One in four (27.8%) adults did not know that tobacco control law bans sell of tobacco products to minors in Turkey. More than half of the participants (57.1%) ever witnessed tobacco sales to minors and 63.6% of them did not act when confronted with the event. Almost all (96.8%) of the respondents thought that access of minors to tobacco products was not difficult. The results of logistic regression of participant’s response against tobacco sales to minor and related factors for current smokers showed that respondents who believed smoking ratio in high school was 4–5 adolescent out of 10 (aOR: 1.59; 95% CI: 1.09–2.34) were more likely to give a warning or informing the police or other people as compared to respondents whose perception on the smoking ratio among high school students was 6–7 adolescents out of 10. The results of logistic regression of non-smokers’ response against tobacco sales to minor were who are from higher educational level, higher economic status, working status and who believed smoking ratio in high school was 4–5 adolescent out of 10 and 2–3 adolescent out of 10 were more likely to give a warning or informing the police or other people as compared to the others. Conclusions Although laws prohibiting the sale of tobacco products to the under age group is very important with regards to accessibility of minors to tobacco products, most of the study participants believed that minors can still easily access tobacco products, and more than half of the participants did not act when confronted with the event. The education, information and monitoring program most especially as it concerns salesman, should be reviewed and strengthened to obey the rules on sales of tobacco products to minors. Education program should be carried out to increase the knowledge and awareness of the community for sale of tobacco to minors. Social sensitivity is important for the prevention of tobacco use and every individual have a responsibility in carrying out this society based program, most especially as it related to prevention of tobacco usage among children and youths.

The European Union Floods Directive requires the establishment of flood maps for high risk areas in all European member states by 2013. However, the current practice of flood mapping in Europe still shows some deficits. Firstly, flood maps are frequently seen as an information tool rather than a communication tool. This means that, for example, local stocks of knowledge are not incorporated. Secondly, the contents of flood maps often do not match the requirements of the end-users. Finally, flood maps are often designed and visualised in a way that cannot be easily understood by residents at risk and/or that is not suitable for the respective needs of public authorities in risk and event management. The RISK MAP project examined how end-user participation in the mapping process may be used to overcome these barriers and enhance the communicative power of flood maps, fundamentally increasing their effectiveness. Based on empirical findings from a participatory approach that incorporated interviews, workshops and eye-tracking tests, conducted in five European case studies, this paper outlines recommendations for user-specific enhancements of flood maps. More specific, recommendations are given with regard to (1) appropriate stakeholder participation processes, which allow incorporating local knowledge and preferences, (2) the improvement of the contents of flood maps by considering user-specific needs and (3) the improvement of the visualisation of risk maps in order to produce user-friendly and understandable risk maps for the user groups concerned. Furthermore, "idealised" maps for different user groups are presented: for strategic planning, emergency management and the public.

We discuss the parsec-scale structural variability of the extragalactic jet 3C111 related to a major radio flux density outburst in 2007. The data analyzed were taken within the scope of the MOJAVE, UMRAO, and F-GAMMA programs, which monitor a large sample of the radio brightest compact extragalactic jets with the VLBA, the University of Michigan 26 m, the Effelsberg 100 m, and the IRAM 30m radio telescopes. The analysis of the VLBA data is performed by fitting Gaussian model components in the visibility domain. We associate the ejection of bright features in the radio jet with a major flux-density outburst in 2007. The evolution of these features suggests the formation of a leading component andmultiple trailing components

Des antigènes solubles obtenus à partir des endozoites de cultures de Besnoitia besnoiti ont été identifiés après leur purification partielle par chromatographie par affinité. Un éluat spécifique obtenu à partir de cette méthode sur une colonne à laquelle avaient été fixés des anticorps provenant du sérum d'une vache naturellement infectée, a montré 7 bandes de polypeptides par électrophorèse (SDS-PAGE). Cinq bandes ont été observées dans l'éluat à partir d'un immuno-adsorbant auquel avaient été couplés des anticorps provenant d'un veau expérimentalement infecté. Les antigènes de l'éluat ont donné une réaction dans le test ELISA. La réactivité des antigènes séparés par électrophorèse avec des sérums bovins inoculés avec les endozoites, et des sérums provenant de cas de besnoitiose contractée sur le terrain, a été étudiée par immunotransfert selon la technique de WESTERN.

L'efficasité des sulfonamides, de la pyriméthamine, du trimothoprim, du lactate d'halofuginone, de l'oxytétracycline, de l'acéturate de dimizène et de la pentamidine a été examinée in vivo lors d'une infection expérimentale fatale à Besnoita besnoiti chez des mérions (Meriones tristami shawii). Une protection complète de l'animal a été obtenue avec une seule dose d'oxytétracycline à longue action injectée par voie intramusculaire à raison de 200 mg/Kg, alors qu'un taux de survie de 50 p. 100 a été observé à la dose de 20 mg/kg. Le traitement à l'oxytétracycline soluble à raison de 25 mg/kg pendant 9 jours consécutifs, a donné des résultats allant jusqu'à 76 p. 100 de survie. Les autres médicaments n'ont pas été efficases. Dans les expériences in vitro, l'oxytétracycline a été totalement inactive sur les endozoïtes B. Besnoiti. Le lactate d'halofiginone seul, chimiquement lié ou melangé aux immunoglobulines provenant de veaux sains, ou infectés par besnoitia, a été éxaminé in vitro dans des milieux de Vero infectés par B. benoiti. Le pourcentage de cellules parasitées a été nettement réduit à 0,5 p. 100pour les cultures traitées avec le médicaments seul, aprés une incubation de 96 heures, alors que le pourcentage était de 54 p. 100 chez les cellules témoins non traitées. La laison ou le mélange d'halofuginone avec les anticorps réduit l'activité du médicament. Cependant, l'inhibition observée dans la multiplication des endozïtes ne peut pas être attribuée à une activité spécifique anti-Besnoitia, puisque une réaction similaire a été obtenue avec des immunoglobulines provenant de bovins sains ou infectés par Besnoitia

Introduction: The intricate roles of immune cells and inflammatory factors in cancer, particularly their association with the risk of bladder cancer, are not well understood., Methods: This study aimed to clarify potential causal relationships between these elements and the development of bladder cancer using genome-wide association study (GWAS) summary statistics for 731 immune cell phenotypes and 91 circulating inflammatory factors (cases=2,053; controls=287,137). The primary analytical approach was Inverse Variance Weighting (IVW), supplemented by MR-Egger regression, weighted median, and weighted mode analyses. Sensitivity analyses included Cochran Q test, MR-Egger intercept test, and Leave-one-out test., Results: The findings indicated that monocytes are positively correlated with an increased risk of bladder cancer. On the contrary, double-negative (DN) T cells, HLA DR+CD8br, and CD28 on CD28+CD45RA+CD8br T cells exhibited an inverse correlation, suggesting a possible protective effect. Furthermore, inflammatory factors IL-20, IL-22RA1, and Eotaxin were significantly associated with an increased risk of bladder cancer., Discussion: These results suggest that certain immune cell phenotypes and inflammatory factors may play a role in the development of bladder cancer and could serve as potential biomarkers for assessing tumor risk. The findings also offer new insights into the pathogenesis of bladder cancer, indicating a need for further investigation., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The reviewer XD declared a shared parent affiliation, with no collaboration, with the authors to the handling editor at the time of the review., (Copyright © 2024 Un, Wusimanjiang, Zhan, Zhang, Li, Lei, Lin, Zhang, Chen and Wang.)

Paracetamol is one of the drugs that cause hepatic damage. Fisetin has wide pharmacological effects such as anticancer, antiinflammatory and antioxidant. We aimed to evaluate the possible protective effect of fisetin on paracetamol-induced hepatotoxicity. Fisetin was administered at 25 and 50 mg/kg doses. Paracetamol was administered orally at a dose of 2 g/kg for induce hepatotoxicity 1 h after the fisetin and NAC treatments. The rats were sacrificed 24h after the Paracetamol administration. Tumor necrosis factor-alpha (TNF-α), NFκB and CYP2E1 mRNA levels and Superoxide dismutase (SOD) activity, glutathione (GSH) and malondialdehyde (MDA) levels of livers were determined. Serum ALT, AST and ALP levels were measured. Histopathological examinations were also performed. Fisetin administration significantly decreased the ALT, AST and ALP levels in a dose dependent manner. In addition, SOD activity and GSH levels increased, and the MDA level decreased with the treatment of fisetin. The TNF-α, NFκB and CYP2E1 gene expressions were significantly lower in both doses of the fisetin groups compared with the PARA group. Histopathological examinations showed that fisetin has hepatoprotective effects. This study showed that fisetin has the liver protective effects by increasing GSH, decreasing inflammatory mediators and CYP2E1.

Background: During the COVID-19 pandemic, elective surgery has to undergo longer wait times, including nephrectomy for T1 renal cell carcinoma (RCC). This study aimed to investigate the time-to-surgery (TTS) of Chinese T1 RCC patients and its influencing factors, and to illustrate the impact of TTS on the prognosis of T1 RCC. Methods: We retrospectively enrolled 762 Chinese patients with pathological T1 RCC that underwent nephrectomy. To discover the impact of TTS on survival outcomes, we explored the possible delay intervals by week using the Kaplan-Meier method and Log-rank test. Cox proportional hazard models with inverse probability-treatment weighting (IPTW) were used to assess the association between TTS and disease-free survival (DFS) and overall survival (OS). Results: The median TTS of T1 RCC patients was 15 days. The Charlson comorbidity index, the Preoperative Aspects and Dimensions Used for an Anatomical (PADUA) score, and the maximal tumor diameter on presentation were independent influencing factors for TTS. The cut-off point of TTS was selected as 5 weeks according to the Log-rank analysis. For T1a RCC, patients with TTS > 5 weeks had similar DFS (HR = 2.39; 95% CI, 0.82−6.94; p = 0.109) and OS (HR = 1.28; 95% CI, 0.23−7.16; p = 0.779) compared to patients with TTS ≤ 5 weeks. For T1b RCC, patients with TTS > 5 weeks had shorter DFS (HR = 2.90; 95% CI = 1.46−5.75; p = 0.002) and OS (HR = 2.49, 95% CI = 1.09−5.70; p = 0.030) than patients with TTS ≤ 5 weeks. Conclusions: Prolonged TTS had no impact on the prognosis of T1a RCC while surgery delayed for over 5 weeks may lead to worse survival in T1b RCC.

Aim: We aimed to investigate the effects of rasagiline on acute lung injury that develops in the sepsis model induced with the cecal ligation and puncture in rats., Main Methods: The rats were separated into the following six groups, Group 1: Sham, Group 2: Sham + Rasagiline 4 mg/kg, Group 3: Sepsis, Group 4: Sepsis + Rasagiline 1 mg/kg, Group 5: Sepsis + Rasagiline 2 mg/kg, Group 6: Sepsis + Rasagiline 4 mg/kg. A total of four holes were opened with a 16-gauge needle through the cecum distal to the point of ligation., Key Findings: Rasagiline treatment increased glutathione level and superoxide dismutase activity while decreased the malondialdehyde level after the sepsis. There was a statistically significant improvement in the doses of 2 mg/kg and 4 mg/kg. Rasagiline also increased Tnf-α, IL1β, IL6, NF-κβand HMGB1 gene expressions in dose-dependent at 2 mg/kg and 4 mg/kg doses. In increased doses, rasagiline prevent the development of edema, the formation of inflammation, and hemorrhage., Significance: Rasagiline exerts both antioxidant and anti-inflammatory effects on the cecal ligation and puncture induced acute lung injury in rats., (© 2022. The Author(s), under exclusive licence to Springer Nature B.V.)

Objectives: We investigated, the effects of aprepitant (APRE) on the lung tissues of rats with an experimental polymicrobial sepsis model (CLP: cecal ligation and puncture) biochemically, molecularly and histopathologically., Methods: A total of 40 rats were divided into 5 groups with 8 animals in each group. Group 1 (SHAM), control group; Group 2 (CLP), cecal ligation and puncture; Group 3 (CLP + APRE10), rats were administered CLP + 10 mg/kg aprepitant; Group 4 (CLP + APRE20), rats were administered CLP + 20 mg/kg aprepitant; and Group 5 (CLP + APRE40), rats were administered CLP + 40 mg/kg aprepitant. A polymicrobial sepsis model was induced with CLP. After 16 h, lung tissues were taken for examination. Tumour necrosis factor α (TNF-α) and nuclear factor-kappa b (NFK-b) messenger ribonucleic acid (mRNA) expressions were analysed by real-time PCR (RT-PCR), biochemically antioxidant parameters such as superoxide dismutase (SOD) and glutathione (GSH) and oxidant parameters such as malondialdehyde (MDA) and lung damage histopathologically., Key Findings and Conclusions: The GSH level and SOD activity increased while the MDA level and the expressions of TNF-α and NFK-b were reduced in the groups treated with APRE, especially in the CLP + APRE40 group. The histopathology results supported the molecular and biochemical results., (© The Author(s) 2021. Published by Oxford University Press on behalf of the Royal Pharmaceutical Society. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)

Aim: Cisplatin (Cis) is widely used chemotherapeutic and has some serious side effects as nephrotoxicity. Phloretin (PH) and Phloridzin (PZ) are known their anti-oxidant anti-inflammatory effects. We aimed to examine the protective effects of PH and PZ on cisplatin-induced nephrotoxicity., Main Methods: Totally, 48 Balb/C female mice were separated into eight groups (n = 6). First day, single dose of cisplatin (20 mg/kg intraperitoneal) was administered to induce toxicity. PH and PZ were given (50 and 100 mg/kg orally) to treatment groups during 3 days. After the experimental procedures serum renal function enzymes (BUN and Creatinine), oxidative parameters (SOD, GSH and MDA), nuclear agent NFKβ, inflammatory cytokines (Tnf-α and IL1β) and HSP70 expressions and histopathological assessments were analyzed., Key Findings: Serum enzymes, tissue cytokines and oxidative stress were increased after the Cis treatment. PH and PZ treatments normalized all parameters compared to Cis administrated group. After the treatments, SOD activities and GSH levels were increased while MDA levels were decreased. PH and PZ treatments decreased Tnf-α, IL1β and NFKβ mRNA expressions. Cis significantly increased the HSP70 expression while PH and PZ administrations significantly decreased. Similar the biochemical and molecular results, PH and PZ showed positive effects on tissue pathological parameters. Cisplatin cause a lot of abnormal structures as tubular and glomeruli damages on the kidney., Significance: PH and PZ play important physiological roles in the prevention of nephrotoxicity. Antioxidant and anti-inflammatory effects of PH and PZ demonstrated visible protective effects in the cisplatin-induced nephrotoxicity model., (Copyright © 2020 Elsevier Inc. All rights reserved.)

Aprepitant is a selective SP/NK-1 receptor antagonist and used in postoperative and chemotherapeutics induced emesis and vomiting. The aim of our study is to show aprepitant may have beneficial effects on gastrointestinal complaints in cancer patients undergoing chemotherapeutics by indomethacin-induced gastric ulcer model. A total of 48 rats were fasted 24 h for ulcer experiment. Aprepitant doses of 5, 10, 20, and 40 mg/kg were evaluated for their antiulcer activity. Omeprazole (20 mg/kg) was used as a positive control group. Six hours after 25 mg/kg indomethacin administration, all stomachs were dissected out. After macroscopic analyses, tumor necrosis factor-alpha (TNF-α), interleukin-1β (IL-1β), COX-1, and COX-2 mRNA levels and SOD activity, and GSH and MDA levels of stomachs were determined. Histopathological examinations were evaluated. Aprepitant administration exerted 48.14%, 49.62%, 65.92%, and 76.77% ulcer inhibition effects at 5, 10, 20, and 40 mg/kg, respectively. Aprepitant administration decreased oxidative stress and inflammatory parameters in stomach tissues dose dependently. Aprepitant administration increased stomach COX-2 mRNA levels at 20 and 40 mg/kg doses. Although aprepitant appears to be disadvantageous in terms of treating gastric ulcer due to COX enzyme inhibition according to the previous studies, aprepitant has been shown to have ulcer healing effect in our study. When aprepitant is given as an anti-nausea and vomiting drug to cancer patients undergoing chemotherapy, we can argue that it will not be necessary to add a new gastric protective agent as it also shows beneficial effects in gastrointestinal complaints.

Objectives: This study aimed to examine the effects of genistein and daidzein on endometrial receptivity by histopathological, immunohistochemical, and biochemical techniques., Materials and Methods: In this study, 72 female Sprague-Dawley rats were randomly divided into 8 groups. The endometrial receptivity model was applied to identified groups. Experimental animals were given periorally 10 mg/kg and high 40 mg/kg doses of genistein and daidzein for 5 days by gavage. At the end of the experiment, uterine tissues were evaluated histopathologically, immunohistochemically, and biochemically., Results: When histopathological findings were examined, significant decreases in pinopod formation were observed in high dose genistein and daidzein groups. When compared with the endometrial receptivity group, immunohistochemical staining findings showed a significant decrease in the expression of integrin β3, integrin αvβ3, LIF, and HOXA10 and an increase in MUC 1 expression in the high dose of genistein and daidzein groups. In biochemical evaluations, it was determined that genistein and daidzein increased estrogen levels and decreased progesterone levels in a dose-dependent manner., Conclusion: Genistein and daidzein have a negative effect on endometrial receptivity. Therefore, individuals with a risk of infertility should pay attention to the consumption of genistein and daidzein.

Aim: Omapatrilat is an antagonist of angiotensin-converting (ACE) and neprilysin-neuropeptidase (NEP) enzymes. The aim of our study is to show that omapatrilat may have beneficial effects as a treatment for polymicrobial sepsis., Main Methods: A cecal ligation and puncture (CLP) sepsis model was used to evaluate 10 and 20 mg/kg doses of omapatrilat in mice (n = 30) fasted for 12 h. The lungs were removed 12 h after CLP, and lung levels of cytokines (tumor necrosis factor-alpha [TNF-α], interleukin-6 [IL-6], NF-κB), iNOS and eNOS mRNA expression, GSH and MDA levels, and ACE and NEP activities were determined. Histopathological examinations were also performed., Key Findings: Omapatrilat treatment provided a dose-dependent reduction in oxidative stress and inflammatory parameters in lung tissues. Omapatrilat administration decreased lung iNOS and eNOS mRNA levels at 20 mg/kg dose. Histopathological analysis revealed a decline in the thickening and edema areas in the alveolar septa in the Sepsis+OMA20 group., Significance: Omapatrilat, a dual ACE and NEP inhibitor, protected lung tissue from sepsis damage by reducing ACE and NEP activities, by decreasing the mRNA expression levels of pro-inflammatory cytokines (TNF-α, IL-6, and NF-κB), by suppressing leukocyte infiltration and edema, by restoring iNOS and eNOS levels, and by restoring SOD activity and GSH and MDA levels, thereby reducing oxidative stress., Competing Interests: Declaration of competing interest The authors declare that there are no conflicts of interest., (Copyright © 2020 Elsevier Inc. All rights reserved.)