Search

Your search keyword '"H. Motta"' showing total 41 results
Start Over Author "H. Motta"
41 results on '"H. Motta"'

3. Desenvolvimento de um método para a determinação de tioconazol associado a nanocápsulas poliméricas por cromatografia líquida

Author

Andréia P. G. Härter, Mariana H. Motta, Júlia G. de Barros, Roseane F. Ribeiro, Andréa I. H. Adams, Scheila R. Schaffazick, and Cristiane de B. da Silva

Subjects
polymeric nanocapsules, tioconazole, validation, Chemistry, QD1-999
Abstract

The aim of this work was to develop and validate an analytical method for the quantification of tioconazole in polymeric nanocapsule suspensions by high performance liquid chromatography with UV detection. The analysis was performed with a mobile phase composed of methanol:water (80:20) and 0.18% ammonium hydroxide; RP-18 column and UV detection at 219 nm. The method proved to be linear in the concentration range of 5-50 µg mL-1 (r = 0.9999), specific, precise (repeatability RSD = 1.42%, intermediate precision RSD = 1.17%), accurate (98 - 102%) and robust (RSD < 2.0%). In conclusion, a simple and rapid method was validated proving suitable for quantification of tioconazole in polymeric nanocapsules.

Published
2014
Full Text
View/download PDF

4. Energy and protein requirements for maintenance of Texel lambs

Author

Carla Joice Härter, Andressa Ana Martins, Stefani Macari, Rafael Sanches Venturini, Cleber Cassol Pires, William Soares Teixeira, J. H Motta, and Sérgio Carvalho

Subjects
Male, growing lambs, retained protein, 040301 veterinary sciences, Randomized block design, Weaning, Biology, Breeding, heat production, Weight Gain, Energy requirement, SF1-1100, 0403 veterinary science, Animal science, Latin square, Animals, Dry matter, net requirements, Sheep, 0402 animal and dairy science, Nutritional Requirements, Proteins, metabolizability, 04 agricultural and veterinary sciences, 040201 dairy & animal science, Animal Feed, Diet, Animal culture, Food restriction, Body Composition, Animal Science and Zoology, Composition (visual arts), Texel, Energy Metabolism, Purebred
Abstract

It can be hypothesized that the body composition characteristics of different sheep breeds affect their nutritional requirements. However, no study has yet been carried out to determine the nutritional requirements for maintenance of Texel purebred lambs, despite their growing importance in sheep meat production globally. Our objective was therefore to determine the energy and protein requirements for maintenance of Texel lambs. Thirty-four Texel lambs were used, all intact males that were weaned at 50 days old, and confined in individual pens. Two experiments were conducted, as follows. In Experiment 1, a digestibility assay was performed to determine the dietary energy value, in a 3×3 double Latin square design, in which lambs were submitted to three levels of feed restriction (0%, 55% and 70% of ad libitum feed intake). In Experiment 2, the energy and protein requirements for maintenance of Texel lambs from 21 to 40 kg BW were determined using a randomized block design, in which lambs were also submitted to three levels of feed restriction (0%, 55% and 70% of ad libitum feed intake). The requirements for net energy for maintenance (NEm), metabolizable energy for maintenance (MEm), net protein for maintenance (NPm) and metabolizable protein for maintenance (MPm) were determined. The digestibility of dry matter, energy, protein and metabolizability were similar between food restriction levels, averaging 74.4%, 75.5%, 80.3% and 0.636, respectively. The NEm determined for growing Texel lambs was 263 kJ/kg of the metabolic fasting BW (FBW), the MEm was 417 kJ/kg0.75 FBW and the efficiency of use of MEm was 0.63. In addition, the NPm was 1.24 g/day per kg0.75 FBW and the MPm was 2.98 g/day per kg0.75 FBW. The energy requirements of Texel lambs are different from those reported in the literature, possibly due to differences between breeds, diets and environmental effects, whereas the protein requirements are different from literature mainly due to methodological differences; further studies are need to address these aspects that affects the nutritional requirements for raising sheep from different breeds in different environments.

Published
2019

6. Sustainability of the Black Press as Social Justice: A Digital Technology Gap Study

Author

Indhira Suero Acosta and Bernardo H. Motta

Subjects
Political science, Sustainability, Public administration, Internet, jornalismo, imprensa negra, jornais Afro-Americanos, Afro-jornais, Social justice, Technology gap
Abstract

The National Newspaper Publishers Association (NNPA) enlists a total of 157 members - publications directed to the African-American community in the United States. There is currently no research on how these publications have adopted technology through time, or if the adoption of new media contributes to their growth and survival in the publishing industry. In Florida, The Weekly Challenger, Daytona Times and Florida Courier, three of 13 historical newspapers, are connected in history and structure and show different types of survival methods. How have these publications adopted technology through time? What types of trends are reflected in these newspapers? What challenges are faced by the Black community weeklies? To answer these questions, the authors conducted case studies employing participant observation, lengthy interviews, historical research and qualitative questionnaires. A significant finding of this research demonstrates the difficulty to get responses due to suspicion and intimidation from the targeted audience. These newspapers struggle with a diminished workforce who lack professional and technical training and must perform multiple roles. Findings also show that 14 of the newspapers listed as current NNPA members are not currently in circulation and that the publications’ pattern of adoption is not planned, but a consequence of availability and chance.

Published
2018

7. Intake and performance of lambs finished in feedlot with wet brewer’s grains

Author

William Soares Teixeira, Sérgio Carvalho, Gustavo Jaurena, Marcelo Machado Severo, M. F. Frasson, A. M. Menegon, and J. H Motta

Subjects
Silage, Veterinary (miscellaneous), 01 natural sciences, Biochemistry, Genetics and Molecular Biology (miscellaneous), Feed conversion ratio, Animal science, Ruminant, medicine, Dry matter, Animal nutrition, Weight gain, lcsh:SF1-1100, Ecology, biology, Research, 010401 analytical chemistry, 0402 animal and dairy science, 04 agricultural and veterinary sciences, Feed conversion, Sorghum, biology.organism_classification, 040201 dairy & animal science, 0104 chemical sciences, Ovines, Feedlot, Animal Science and Zoology, lcsh:Animal culture, medicine.symptom, Food Science
Abstract

Background The use of agroindustrial by-products in ruminant nutrition to be an interesting alternative in order to reduce production costs and environmental impacts arising from the inadequate destination of residues. The initial step of beer production yields a large volume of wet brewer’s grains all year around, which is available at a low cost and has a high nutritional quality, and hence a big potential for animal production. Methods Twenty-four Suffolk non-castrated male lambs, from simple parturition were kept in individual spots and allocated to four treatments constituted by four levels of substitution of sorghum silage by WBG (i.e.0; 33.5; 66.5 and 100% of substitution). It was used roughage: concentrate rate of 50:50, based on dry matter. Results The ether extract intake increased while the acid detergent fiber intake decreased linearly (P ≤ 0.05). Substituion of sorghum silage by WBG did not change lambs’ feed DMI, daily weight gain and feed conversion. Conclusion The substitution of sorghum silage by WBG as roughage showed to be a viable alternative from the productive and economic point of view for finishing of feedlot lambs.

Published
2018

8. Características da carcaça e composição tecidual da carne cordeiros confinados com o uso de dietas de alto grão

Author

Vagner Lopes Mello, G. M. C Bernardes, Rafael Sanches Venturini, André Cocco Pesamosca, William Soares Teixeira, Sérgio Carvalho, J. H Motta, Luiza Ilha Borges, Juliene da Silva Rosa, and Matheus Lehnhart de Moraes

Subjects
animal structures, Fat content, animal diseases, food and beverages, Biology, Subcutaneous fat, Husk, CORN GRAIN, Animal science, Feedlot, General Agricultural and Biological Sciences, Texel, Tissue composition, Consumer market
Abstract

This study aimed to evaluate the effect of using different high-grain diets on the carcass characteristics and tissue composition of the meat of feedlot-finished lambs. Thirty-two male castrated Texel lambs born from single births and weaned at approximately 50 days of age were allotted to treatments that consisted of different unprocessed grains, as follows: corn grain, white oat grain, black oat grain, or grain of rice in the husk. The animals were slaughtered upon reaching the pre-established slaughter weight of 32 kg, which corresponds to 60% of the mature weight of their mothers. Lambs fed the high-corn grain diet had higher weights and yields of hot and cold carcass and larger loin-eye areas than those finished in the feedlot receiving high-grain diets based on white oat, black oat, or rice in the husk. Additionally, they had a higher degree of fatness and a thicker subcutaneous fat layer at slaughter, which lead to decreased cooler shrink loss. The neck, shoulder, ribs, and leg cuts were heavier in lambs fed the high-corn grain diet. By contrast, these lambs had a lower proportion of leg, more rib, and higher meat fat content, which may be undesirable for the consumer market. The use of high-corn grain diets, in comparison with high-grain diets based on black oat, white oat, and rice in the husk, may be recommended for finishing lambs in the feedlot when they are slaughtered at similar weights.

Published
2018

9. Few data but many fish: marine small-scale fisheries catches for Mozambique and Tanzania

Author

Helen E. Fox, Dirk Zeller, H. Motta, A. Ngusaru, and Jennifer Jacquet

Subjects
Food security, biology, business.industry, Fishing, Qualitative property, Aquatic Science, biology.organism_classification, Fishery, Geography, Tanzania, Agriculture, Scale (social sciences), Mainland, Fisheries management, business, Ecology, Evolution, Behavior and Systematics
Abstract

The fisheries data supplied to the Food and Agriculture Organization of the United Nations (FAO) by national agencies have served as the primary tool for many global and regional studies. However, it is recognised that these data are incomplete and often underestimate actual catches, particularly for small-scale fisheries. This study reconstructed total marine fisheries catches from 1950 to 2005 for Mozambique and the United Republic of Tanzania, by applying an established catch reconstruction approach utilising all available quantitative and qualitative data, combined with assumption-based estimations and interpolations. Since the 1950s, Mozambique has reported primarily industrial catches and has substantially under-reported the country's small-scale fishing sector due to lack of resources and civil war. In Tanzania, Zanzibar's recorded fisheries statistics prior to 2000 are absent from Tanzania's marine fisheries catches reported to FAO, and total mainland catches are at least one-third larger than off...

Published
2010

10. Effect of single and multiple overloading on the residual fatigue life of a structural steel

Author

M. S. Ramos, M. V. Pereira, F. A. Darwish, S. H. Motta, and M. A. Carneiro

Subjects
Materials science, Growth retardation, business.industry, Mechanical Engineering, Work (physics), Fracture mechanics, Structural engineering, Paris' law, Residual, Crack closure, Mechanics of Materials, Residual stress, General Materials Science, business, Stress concentration
Abstract

This work was carried out to determine the effect of overload cycles on the fatigue life of a structural steel used for offshore applications. Single and multiple overloads were adopted and the corresponding fatigue crack growth retardation was evaluated. Residual stress fields were measured in the vicinity of the crack tip using an X-ray diffraction technique and their size compared with that of the overload cyclic plastic zone. In regard to crack growth retardation, the results indicated that the extension in fatigue life increases with an increase in overload, as a consequence of the generation of higher compressive residual stress levels over a larger distance ahead of the crack tip. The effect of two equal and consecutive overloads, with the second one applied at different intervals of crack propagation from the first, was also considered. Larger intervals were shown to lead to a longer residual fatigue life.

Published
2003

11. Automated screening of conventional gynecological cytology smears: feasible and reliable

Author

Tania H. Motta, Cristovam Scapulatempo, Monica M.A. Stiepcich, Mauro Ajaj Saieg, Maria E. Fodra, Adhemar Longatto-Filho, and Universidade do Minho

Subjects
Adult, medicine.medical_specialty, Histology, Adolescent, Uterine Cervical Neoplasms, Risk Assessment, Pathology and Forensic Medicine, Workflow, Young Adult, Automation, Predictive Value of Tests, Risk Factors, Cytology, Image Interpretation, Computer-Assisted, Conventional smears, Medicine, Humans, Medical physics, Aged, Retrospective Studies, Colposcopy, Gynecology, Aged, 80 and over, Automation, Laboratory, Vaginal Smears, Science & Technology, medicine.diagnostic_test, business.industry, Gynecological cytology, Reproducibility of Results, General Medicine, Middle Aged, Prognosis, 3. Good health, Feasibility Studies, Female, business, Precancerous Conditions
Abstract

Objectives: We tested the ability of automated screening in processing conventional gynecological cytology smears and its efficacy in assessing sample adequacy and stratifying cases for risk of malignancy. Study Design: Cases were retrospectively selected, including unsatisfactory samples and slides with various sorts of artifacts. Automated screening was performed using the FocalPoint GS Imaging System (Becton Dickinson, Franklin Lakes, N.J., USA), with classification into five quintiles. For agreement purposes, cases were grouped into high risk for malignancy (quintiles 1 and 2) and low risk for malignancy (quintiles 3, 4 and 5). Results: A total of 120 cases (median age 37.5 years, range 18-85) were included in the study. Eighty-three cases (69.2%) could be successfully classified into quintiles. When divided by risk, 31 cases were placed in the high-risk and 52 in the low-risk group. The overall sensitivity and specificity of the automated analysis was 100 and 70.3%, respectively. Conclusions: Automated analysis could analyze the majority of conventional smears, including one case previously screened as unsatisfactory. All malignant and high-grade lesions were correctly classified into the high-risk group. Broad use of this automation system could potentially decrease screening time and augment the efficacy in detecting precursor neoplastic changes in cervical cytology smears.

Published
2014

12. Recognition of altered segments in Brazilian Sign Language

Author

Verdu,Ana Claudia M. Almeida, Caneguim,Janaína de Fatima Castro, Rose,Júlio C. de, and Bandini,Heloisa H. Motta

Subjects
segments of signs, Brazilian Sign Language, adolescents, discrimination
Abstract

The purpose of this study was to verify discriminative control by segments of signs in adolescents with deafness who use Brazilian Sign Language (BSL). Four adolescent with bilateral deafness, with 3 years of BSL teaching, saw a video presenting a children's tale in BSL. After showing accurate understanding of the story, participants saw another video of the same story with 12 signs altered in one of their segments (hand configuration, place of articulation, or movement). They apparently did not detect the alterations. However, when the signs were presented in isolation in a matching-to-sample test, they virtually always selected the picture corresponding to the unaltered signs. Three participants selected an unfamiliar picture in 50% or more trials with an altered sign as a sample, showing that they could detect the majority of the altered signs.

Published
2012

13. Control strategies applied to autonomous underwater vehicle for inspection of dams

Author

Deisson Cassiano Diedrich, Carlos H. F. dos Santos, Antonio C. B. Chiella, Jeremy G. Rauber, and Lucas R. H. Motta

Subjects
Engineering, Hydroelectricity, business.industry, Control (management), Trajectory, Mobile robot, Feedback linearization, Energy supply, Underwater, business, Maintenance engineering, Marine engineering
Abstract

Hydroelectric power plants need to ensure an uninterrupted energy supply. To achieve this objective, activities of maintenance and inspection are done periodically. The use of divers to do these maintenance in submerged areas, implied high risks for them. In this sense, the automated inspection of such facilities through underwater vehicles can be a satisfactory solution. This article presents a comparison between two control techniques applied to underwater vehicle, Feedback Linearization and Sliding Mode. These strategies are responsible to ensure the following two requirements. First, to make the vehicle overcome the velocity gradient caused by dam's water intakes. Second, to follow the desired trajectory. The control laws were tested by analysing their performance in a helical reference trajectory with the presence of disturbance caused by water intakes.

Published
2012

16. Multiple jet production at low transverse energies inpp¯collisions ats=1.8TeV

Author

V. V. Babintsev, G. Ginther, Jianming Qian, L. Duflot, V. Simak, B. Kothari, P. Padley, Alexey Volkov, G. Briskin, N.V. Mokhov, A. Leflat, Jeffrey F. Krane, M. I. Martin, Elemer Nagy, A. P. Heinson, P. Demine, Mitchell Wayne, H. Schellman, J. M. Kohli, H. Zheng, Yu-tin Huang, E. Kajfasz, R. E. Hall, M. Sosebee, D. Edmunds, Stefan Grünendahl, Elizabeth Gallas, T. McMahon, D. Coppage, J. T. Eltzroth, R. Jesik, K. A. Johns, Z. Casilum, Nikos Varelas, Andre Sznajder, S. Tentindo-Repond, Christos Leonidopoulos, Bing Zhou, R. K. Shivpuri, L. Lueking, Anurag Gupta, Mossadek Talby, B. Gómez, Pushpalatha C Bhat, Yuri Gershtein, H. L. Melanson, S. Krzywdzinski, Freya Blekman, Wendy Taylor, D. R. Claes, A. Patwa, V. Oguri, H. E. Fisk, T. C. Bacon, Philip Baringer, Shashikant Dugad, H. Motta, S. Fu, Gustaaf Brooijmans, D. Buchholz, P. Krivkova, J. Warchol, S. Doulas, J. Womersley, A. Zylberstejn, F. Lehner, H. Greenlee, B. M. Sabirov, S. Banerjee, Z. Zhou, J. Solomon, N. A. Naumann, J. H. Christenson, V. M. Abazov, Kaushik De, Neeti Parashar, M. A.C. Cummings, Florian Beaudette, B. Hoeneisen, Lee Sawyer, L. Babukhadia, M. A. Strang, Andrew White, Roger Moore, D. P. Stoker, Q. Xu, David H. Adams, Alice Bean, D. A. Stoyanova, B. G. Pope, E. Popkov, D. Karmanov, C. S. Mishra, Andrew Brandt, R. Engelmann, M. Zielinski, R. L. McCarthy, A. S. Turcot, S. Reucroft, R. Gilmartin, F. Canelli, Volker Buescher, S. M. Tripathi, M. Gao, Gregory R Snow, B. Knuteson, Ia Iashvili, E. A. Kozlovsky, H.E. Miettinen, Suyong Choi, A. Goussiou, A. V. Kostritskiy, M. Bhattacharjee, B. Connolly, Richard Breedon, K. Gounder, Vipin Bhatnagar, Sissel Hansen, R. Van Kooten, G. Obrant, D. Mihalcea, U. Bassler, A. Bross, A. Besson, O. Peters, Andrei Nomerotski, Haiyan Wang, S.A. Kahn, Elizaveta Shabalina, Vasken Hagopian, R. Partridge, X. Zhang, T. Rockwell, Iain Alexander Bertram, M. Demarteau, T. G. Trippe, G. A. Davis, Serban Protopopescu, M. Strovink, P.F. Ermolov, Bobby Samir Acharya, H. A. Neal, H. Weerts, M. Roco, Z. M. Wang, John Hobbs, L. Coney, M. Merkin, Stephen Wimpenny, Michael Begel, V. L. Malyshev, Lev Dudko, D. Fein, P. M. Tuts, V. E. Kuznetsov, R. W. Stephens, Michael Rijssenbeek, D. Chakraborty, A. Zieminski, J. T. White, J. T. Linnemann, W. Kahl, E. G. Zverev, V. Sorín, K. Yip, Sharon Hagopian, Don Lincoln, P. W. Balm, J. Barreto, V. S. Burtovoi, T. Marshall, G. Eppley, D. A. Wijngaarden, H. S. Mao, K. Papageorgiou, Alberto Santoro, A. Stone, Daniel John Karmgard, C. Hebert, Heriberto Castilla-Valdez, M. Buehler, Darien Wood, K. Bos, Seong Keun Kim, J. G.R. Lima, Gregorio Bernardi, D. Denisov, P. Yamin, Nicholas John Hadley, Robert Hirosky, L. Stutte, Raymond Brock, Boaz Klima, N. R. Stanton, G. D. Alexeev, Flera Rizatdinova, A.S. Dyshkant, V. N. Evdokimov, R. A. Sidwell, Michael A. Strauss, P. Rubinov, J. M. Heinmiller, Srinivasan Rajagopalan, R. Beuselinck, S. Blessing, Hal Evans, Scott Snyder, Thomas Ferbel, A. M. Kalinin, D. Bauer, Jing Li, R. Demina, Meenakshi Narain, S. J. Jong, A. S. Ito, S. Willis, R. Piegaia, G. Gutierrez, Gilvan Alves, Vladimir Gavrilov, Gordon Watts, F. Fleuret, Y. Kulik, E. Flattum, R. J. Madaras, E. W. Anderson, L. S. Vertogradov, A. N. Galyaev, Alexander Belyaev, V. Sirotenko, R. Snihur, D. Casey, Y. Fisyak, J. Kotcher, G. Steinbrück, O. V. Eroshin, Y. Song, Marvin Johnson, Suman Bala Beri, S. Chopra, Q. Z. Li, P. A. Rapidis, D. K. Cho, Y. Arnoud, Patrick Slattery, K. Genser, Phillip Gutierrez, Harald Fox, A. V. Kozelov, A. Jonckheere, R. Illingworth, W. E. Cooper, S. Duensing, V. S. Narasimham, L. Groer, Ariel Schwartzman, A. Juste, A. P. Vorobiev, Stephan Linn, Rajendran Raja, A. Alton, N. Graf, N. Oshima, Daria Zieminska, Y. D. Mutaf, A. Khanov, N. Parua, N. Sotnikova, V.V. Abramov, Melissa Ridel, R. Kehoe, R. D. Schamberger, Arnaud Duperrin, A. A. Mayorov, D. Hedin, Ulrich Heintz, M. A. Kubantsev, R. J. Genik, M. Jaffré, M. H.G. Souza, Jong-Sung Yu, Brad Abbott, Shuichi Kunori, H. Jöstlein, Chris Hays, Frank Filthaut, V. A. Bezzubov, Christophe Royon, Pierre Petroff, Arnaud Lucotte, M. Diesburg, Alexander Kupco, J. P. Negret, R. P. Smith, Sebastian Grinstein, H. E. Montgomery, V. Vaniev, J. Snow, K. M. Chan, Michael Shupe, Michael Hildreth, Randy Ruchti, H. D. Wahl, M. Zanabria, Winston Ko, J. Estrada, Y. A. Yatsunenko, Harrison Prosper, S. Desai, T. Yasuda, S. N. Ahmed, M. Fortner, N. W. Reay, Emanuela Barberis, L. T. Goss, Charles Leggett, Tulika Bose, H. Haggerty, M. B. Przybycien, F. Borcherding, N. I. Bojko, G. Sajot, D. O'Neil, J. N. Butler, P. D. Grannis, P. I. Goncharov, C. Miao, Miguel Mostafa, B. Olivier, S. Youssef, M. Abolins, Sarah Catherine Eno, N. K. Mondal, H. T. Diehl, T. A. Bolton, H. Singh, D. Whiteson, A. Boehnlein, J. Ellison, A. K.A. Maciel, Sa. Jain, S. Fuess, A. Abdesselam, V. Zutshi, Zeno Dixon Greenwood, S. P. Denisov, M. R. Krishnaswamy, D. Shpakov, A. R. Clark, Sergey Chekulaev, K. W. Merritt, T. Nunnemann, B. Baldin, J. F. Bartlett, V. Manankov, Y. Ducros, W. M. Lee, A. Kharchilava, F. Nang, Richard B. Lipton, Howard Gordon, Sergey Kuleshov, F. Villeneuve-Seguier, Victor Daniel Elvira, D. Cutts, Wagner Carvalho, Greg Landsberg, K. Soustruznik, A. Baden, G. C. Blazey, R. Yamada, John Rutherfoord, C. Luo, Mary Beth Adams, S. N. Gurzhiev, Viatcheslav Stolin, James C. Green, C. Lundstedt, Carlos Avila, Sabine Crépé-Renaudin, J. M. Hauptman, and Cecilia Elena Gerber

Subjects
Physics, Quantum chromodynamics, Nuclear and High Energy Physics, Jet (fluid), Particle physics, 010308 nuclear & particles physics, Astrophysics::High Energy Astrophysical Phenomena, High Energy Physics::Phenomenology, 01 natural sciences, 7. Clean energy, Spectral line, Nuclear physics, Transverse plane, 0103 physical sciences, High Energy Physics::Experiment, Production (computer science), Nuclear Experiment, 010306 general physics, Bar (unit)
Abstract

We present data on multiple production of jets with transverse energies near 20 GeV in p (p) over bar collisions at roots=1.8 TeV. QCD calculations in the parton-shower approximation of PYTHIA and HERWIG and the next-to-leading order approximation of JETRAD are compared to the data for one, two, three, and four jet inclusive production. Transverse energy spectra and multiple jet angular and summed transverse-energy distributions are adequately described by the shower approximation while next-to-leading order calculations describe the data poorly.

Published
2003

17. tt¯production cross section inpp¯collisions ats=1.8TeV

Author

M. Bhattacharjee, S. J. Jong, H. D. Wahl, M. Zanabria, Winston Ko, T. G. Trippe, Chris Hays, T. Fahland, Vipin Bhatnagar, Sharon Hagopian, K. Papageorgiou, V. A. Bezzubov, J. P. Negret, T. Yasuda, D. Buchholz, J. M. Hauptman, Cecilia Elena Gerber, Wendy Taylor, A. Patwa, R. P. Smith, A. Khanov, Alexander Belyaev, N. Parua, Roger Moore, N. Sotnikova, R. J. Genik, P. Tamburello, Heng Xu, Florian Beaudette, R. Partridge, X. Zhang, V.V. Abramov, Heriberto Castilla-Valdez, R. Piegaia, Patrick Slattery, Michael Shupe, Michael Hildreth, V. V. Babintsev, P. A. Rapidis, G. Ginther, V. L. Malyshev, M. Jaffré, D. Fein, D. Chakraborty, R. Snihur, Gregory R Snow, Robert Hirosky, S. Fu, J. Estrada, S. P. Denisov, H. S. Mao, Jianming Qian, A. M. Kalinin, S. M. Chang, A. Jonckheere, P.F. Ermolov, Arnaud Lucotte, D. Bauer, R. Demina, A. Alton, P. Demine, H. Zheng, Q. Xu, M. R. Krishnaswamy, Alexander Kupco, Emanuela Barberis, L. T. Goss, Charles Leggett, T. C. Bacon, Elizabeth Gallas, T. McMahon, E. Flattum, A. P. Vorobiev, Stephan Linn, N. Oshima, Daria Zieminska, Mark Johnson, R. D. Schamberger, V. S. Burtovoi, A. R. Clark, G. Gutierrez, Gilvan Alves, G. Briskin, Jeffrey F. Krane, Ulrich Heintz, H. Jöstlein, Y. A. Yatsunenko, A. V. Kotwal, Harrison Prosper, D. P. Stoker, D. Coppage, Anurag Gupta, Randy Ruchti, Zeno Dixon Greenwood, H. L. Melanson, Sergey Chekulaev, Frank Filthaut, S. Desai, M. A. Kubantsev, Tulika Bose, V. M. Abazov, S. Chopra, Don Lincoln, Erich Varnes, P. W. Balm, A. Stone, Daniel John Karmgard, Pushpalatha C Bhat, Yu-tin Huang, M. Diesburg, G. Steinbrück, K. Genser, J. H. Christenson, David H. Adams, K. M. Chan, B. M. Sabirov, V. S. Narasimham, D. A. Stoyanova, M. A.C. Cummings, C. Hebert, Rajendran Raja, N. A. Naumann, Alice Bean, A. S. Turcot, H. Haggerty, B. Hoeneisen, Lee Sawyer, L. Groer, J. T. Eltzroth, R. J. Madaras, Nikos Varelas, Bing Zhou, B. Knuteson, T. Rockwell, Iain Alexander Bertram, R. L. McCarthy, Brad Abbott, M. Ridel, Stephen Wimpenny, F. Canelli, K. W. Merritt, R. Jesik, Laurent Duflot, Haiyan Wang, S.A. Kahn, Michael Rijssenbeek, S. Reucroft, Y. Ducros, M. Abolins, Pierre Petroff, K. A. Johns, Michael Begel, A. A. Mayorov, Volker Buescher, J. F. Bartlett, M. Demarteau, C. Yoshikawa, R. Van Kooten, A. Besson, T. Marshall, V. Manankov, U. Bassler, D. Stewart, Ariel Schwartzman, E. Smith, E. J. Ramberg, S. N. Gurzhiev, M. H.G. Souza, V. N. Evdokimov, A. Nomerotski, Jing Li, A. Zylberstejn, J. Snow, S. N. Ahmed, M. Fortner, Michael A. Strauss, P. Yamin, A. Zieminski, H. E. Montgomery, W. Kahl, G. D. Alexeev, Flera Rizatdinova, M. Roco, Darien Wood, N. W. Reay, N. K. Mondal, H. T. Diehl, G. Eppley, T. A. Bolton, Nicholas John Hadley, V. Oguri, E. G. Zverev, Y. Fisyak, J. G.R. Lima, S. Blessing, H. Singh, N.V. Mokhov, M. Chung, D. Whiteson, A. Boehnlein, Y. Kulik, M. I. Martin, Elemer Nagy, Scott Snyder, Gustaaf Brooijmans, L. Lueking, R. Kehoe, J. Ellison, A. K.A. Maciel, A. P. Heinson, Sa. Jain, S. Fuess, A. V. Kozelov, A. S. Ito, Shuichi Kunori, R. K. Shivpuri, E. Popkov, V. Vaniev, J. Warchol, D. Hedin, Suman Bala Beri, Matthew Jones, Viatcheslav Stolin, Christos Leonidopoulos, D. R. Claes, Meenakshi Narain, P. I. Goncharov, C. Cretsinger, M. Strovink, R. E. Hall, J. Womersley, S. Doulas, D. Mihalcea, Z. Casilum, A. Juste, C. Miao, B. Olivier, M. B. Przybycien, Andrew White, C. Royon, P. M. Tuts, B. Pawlik, Howard Gordon, V. E. Kuznetsov, Kaushik De, E. W. Anderson, S. Krzywdzinski, B. Kothari, K. C. Frame, R. W. Stephens, D. Karmanov, Q. Z. Li, M. Sosebee, C. Lundstedt, A. Goussiou, D. Edmunds, D. Casey, S. M. Tripathi, J. Kotcher, F. Lehner, H. Greenlee, Carlos Avila, N. Amos, Sebastian Grinstein, Ia Iashvili, E. Amidi, Harald Fox, Andre Sznajder, A. Bross, Sabine Crépé-Renaudin, A. V. Kostritskiy, K. Gounder, Yuri Gershtein, Sissel Hansen, L. S. Vertogradov, O. Peters, John Hobbs, J. Bantly, Freya Blekman, N. Graf, A. N. Galyaev, G. A. Davis, Serban Protopopescu, L. Coney, Suyong Choi, N. Sen, Y. D. Mutaf, J. Barreto, H. Motta, E. Kajfasz, P. Krivkova, Bobby Samir Acharya, H. A. Neal, J. T. Linnemann, V. Simak, P. Padley, L. Babukhadia, Alexey Volkov, D. K. Cho, Y. Arnoud, James H Cochran, V. Sorín, K. Yip, R. Illingworth, Mitchell Wayne, H. Schellman, J. M. Kohli, Stefan Grünendahl, J. Thompson, B. Connolly, Mossadek Talby, Richard Breedon, B. Gómez, L. Stutte, Z. H. Zhu, Shashikant Dugad, Andrew Brandt, W. E. Cooper, R. Engelmann, A.S. Dyshkant, S. Duensing, E. A. Kozlovsky, H.E. Miettinen, M. A. Strang, C. S. Mishra, F. Fleuret, Arnaud Duperrin, D. A. Wijngaarden, A. Abdesselam, K. Bos, Gregorio Bernardi, D. Denisov, Jong-Sung Yu, Vasken Hagopian, H. Weerts, Z. M. Wang, Lev Dudko, J. M. Heinmiller, Srinivasan Rajagopalan, Hal Evans, Thomas Ferbel, M. Merkin, Vladimir Gavrilov, Gordon Watts, Raymond Brock, Boaz Klima, R. Beuselinck, V. Sirotenko, O. V. Eroshin, Y. Song, Phillip Gutierrez, R. A. Sidwell, S. Willis, Neeti Parashar, B. G. Pope, M. Zielinski, N. I. Bojko, D. O'Neil, Elizaveta Shabalina, J. N. Butler, P. D. Grannis, J. T. White, S. Youssef, V. Zutshi, A. Leflat, D. Shpakov, Eunil Won, W. G. Cobau, T. Nunnemann, B. Baldin, S. Tentindo-Repond, W. M. Lee, A. Kharchilava, Sergey Kuleshov, Victor Daniel Elvira, Philip Baringer, S. Banerjee, V. Balamurali, M. Buehler, N. R. Stanton, Alberto Santoro, Seong Keun Kim, P. Rubinov, H. E. Fisk, L. J. Pan, Z. Zhou, J. Solomon, R. Gilmartin, M. Gao, A. Baden, G. C. Blazey, R. Yamada, John Rutherfoord, C. Luo, Mary Beth Adams, James C. Green, D. Cutts, Wagner Carvalho, Greg Landsberg, M. Paterno, K. Soustruznik, F. Borcherding, G. Sajot, Miguel Mostafa, M. M. Baarmand, Sarah Catherine Eno, F. Nang, Richard B. Lipton, and F. Villeneuve-Seguier

Subjects
Physics, Nuclear and High Energy Physics, Cross section (physics), Particle physics, 010308 nuclear & particles physics, 0103 physical sciences, Production (computer science), 010306 general physics, 01 natural sciences
Published
2003

18. Search for minimal supergravity in single-electron events with jets and large missing transverse energy inpp¯collisions ats=1.8TeV

Author

P.F. Ermolov, E. Flattum, S. Chopra, Ulrich Heintz, Ariel Schwartzman, E. Smith, Frank Filthaut, Raymond Brock, Boaz Klima, R. Beuselinck, A. Khanov, N. Parua, V.V. Abramov, V. S. Burtovoi, R. K. Shivpuri, D. R. Claes, V. Simak, P. Padley, Alexey Volkov, S. Krzywdzinski, M. Diesburg, K. Genser, M. Jaffré, Rajendran Raja, Brad Abbott, Pierre Petroff, R. Kehoe, S. Willis, R. J. Madaras, S. Doulas, Kaushik De, Mitchell Wayne, H. Schellman, J. M. Kohli, Stefan Grünendahl, Mossadek Talby, B. Gómez, P. I. Goncharov, Suyong Choi, C. Miao, R. Van Kooten, A. Besson, L. S. Vertogradov, A. N. Galyaev, Arnaud Lucotte, Alexander Kupco, S. N. Ahmed, M. Fortner, N. W. Reay, Meenakshi Narain, T. Nunnemann, B. Baldin, H. A. Neal, A. Zieminski, W. Kahl, F. Lehner, H. Greenlee, Andrew White, D. K. Cho, Y. Arnoud, R. Illingworth, D. Buchholz, S. M. Tripathi, M. Abolins, P. A. Rapidis, R. A. Sidwell, Randy Ruchti, B. Kothari, W. M. Lee, A. Kharchilava, Florian Beaudette, Sergey Kuleshov, V. Sorín, K. Yip, D. Edmunds, A. Bross, O. Peters, W. E. Cooper, S. Duensing, N. Sotnikova, A. Goussiou, S. Fu, R. J. Genik, S. J. Jong, Victor Daniel Elvira, L. Stutte, A.S. Dyshkant, Shuichi Kunori, Andrei Nomerotski, N. K. Mondal, H. T. Diehl, T. A. Bolton, H. Singh, Wendy Taylor, A. Patwa, Arnaud Duperrin, Gregory R Snow, Andre Sznajder, G. A. Davis, Serban Protopopescu, Jong-Sung Yu, L. Lueking, John Hobbs, S. Youssef, J. Krane, M. Sosebee, F. Fleuret, A. V. Kostritskiy, R. D. Schamberger, K. Gounder, Roger Moore, J. Barreto, D. Hedin, Sissel Hansen, S. R. Dugad, V. Vaniev, V. Zutshi, D. Whiteson, A. Boehnlein, T. G. Trippe, M. A. Strang, M. Buehler, L. Coney, Yuri Gershtein, E. Kajfasz, D. Fein, Alexander Belyaev, J. Ellison, A. K.A. Maciel, M. B. Przybycien, N. Sen, C. S. Mishra, M. R. Adams, Chris Hays, V. A. Bezzubov, J. P. Negret, Christos Leonidopoulos, P. Demine, Freya Blekman, H. Motta, Sa. Jain, S. Fuess, D. Chakraborty, P. Krivkova, Don Lincoln, H. Zheng, D. Shpakov, R. L. McCarthy, Sharon Hagopian, K. Papageorgiou, M. H.G. Souza, Patrick Slattery, Elizabeth Gallas, T. McMahon, H. Jostlein, N. I. Bojko, N. R. Stanton, R. P. Smith, D. O'Neil, Howard Gordon, U. Bassler, D. Coppage, D. A. Stoyanova, P. W. Balm, A. Stone, Daniel John Karmgard, L. Babukhadia, K. C. Frame, A. Jonckheere, E. Popkov, H. E. Montgomery, E. W. Anderson, A. Baden, D. Denisov, A. S. Turcot, Yu-tin Huang, J. Womersley, Michael Shupe, Michael Hildreth, Vasken Hagopian, G. Steinbrück, A. P. Vorobiev, Stephan Linn, N. A. Naumann, H. Weerts, Andrew Brandt, R. Engelmann, C. Hebert, Q. Z. Li, G. C. Blazey, Z. M. Wang, B. Olivier, Kenneth Johns, Richard Breedon, B. S. Acharya, J. N. Butler, P. D. Grannis, R. Yamada, A. Zylberstejn, J. Estrada, J. M. Heinmiller, Srinivasan Rajagopalan, Hal Evans, Thomas Ferbel, E. A. Kozlovsky, H.E. Miettinen, Michael Begel, M. Ridel, S. Reucroft, Nikos Varelas, R. Piegaia, R. Snihur, S. P. Denisov, Alberto Santoro, M. Roco, Sebastian Grinstein, Lev Dudko, F. Villeneuve-Seguier, G. Eppley, H. Haggerty, Bing Zhou, N. Oshima, A. V. Kotwal, Harrison Prosper, S. Desai, John Rutherfoord, J. G.R. Lima, G. Bernardi, Emanuela Barberis, D. Karmanov, Q. Xu, Christophe Royon, Zeno Dixon Greenwood, A. Alton, D. Mihalcea, M. R. Krishnaswamy, Heriberto Castilla-Valdez, Daria Zieminska, V. M. Abazov, Y. Fisyak, L. T. Goss, Charles Leggett, T. Rockwell, Iain Alexander Bertram, C. Luo, J. T. Eltzroth, P. Rubinov, J. M. Hauptman, M. A. Kubantsev, Stephen Wimpenny, T. Marshall, H. D. Wahl, M. Zanabria, Winston Ko, E. G. Zverev, Ia Iashvili, Darien Wood, R. Jesik, Laurent Duflot, P. M. Tuts, V. E. Kuznetsov, R. W. Stephens, Tulika Bose, Jing Li, Cecilia Elena Gerber, Michael Rijssenbeek, N.V. Mokhov, A. S. Ito, Nicholas John Hadley, Vladimir Gavrilov, E. J. Ramberg, M. I. Martin, M. Merkin, K. M. Chan, A. R. Clark, Robert Hirosky, V. V. Babintsev, Gordon Watts, S. Blessing, V. Sirotenko, A. Leflat, Sergey Chekulaev, Marvin Johnson, A. M. Kalinin, A. P. Heinson, G. Ginther, Scott Snyder, O. V. Eroshin, Y. Song, Phillip Gutierrez, D. A. Wijngaarden, A. Abdesselam, P. Yamin, D. Bauer, G. D. Alexeev, Flera Rizatdinova, K. Bos, James C. Green, M. Chung, Y. Ducros, A. V. Kozelov, T. Yasuda, V. Oguri, R. Demina, S. Tentindo-Repond, R. E. Hall, Michael A. Strauss, G. Gutierrez, Gilvan Alves, Gustaaf Brooijmans, V. N. Evdokimov, Z. Casilum, J. T. Linnemann, Neeti Parashar, Jianming Qian, D. Cutts, K. W. Merritt, J. Warchol, Philip Baringer, J. F. Bartlett, S. Banerjee, V. S. Narasimham, L. Groer, V. Manankov, M. Zielinski, T. C. Bacon, Wagner Carvalho, B. G. Pope, G. Briskin, Y. Kulik, A. A. Mayorov, Greg Landsberg, D. P. Stoker, J. Snow, S. N. Gurzhiev, Elizaveta Shabalina, B. Connolly, Suman Bala Beri, Y. A. Yatsunenko, J. T. White, A. Juste, Viatcheslav Stolin, C. Lundstedt, Carlos Avila, M. Strovink, Sabine Crépé-Renaudin, M. Paterno, K. Soustruznik, M. Bhattacharjee, H. E. Fisk, L. J. Pan, Z. Zhou, J. Solomon, F. Borcherding, R. Gilmartin, M. Gao, G. Sajot, B. M. Sabirov, Miguel Mostafa, M. M. Baarmand, Sarah Catherine Eno, Anurag Gupta, F. Nang, Richard B. Lipton, Elemer Nagy, Vipin Bhatnagar, R. Partridge, X. Zhang, V. L. Malyshev, H. S. Mao, B. Pawlik, D. Casey, J. Kotcher, Harald Fox, N. Graf, Y. D. Mutaf, H. L. Melanson, S. K. Kim, J. H. Christenson, David H. Adams, Alice Bean, F. Canelli, Volker Buescher, Pushpalatha C Bhat, M. A.C. Cummings, B. Hoeneisen, Lee Sawyer, B. Knuteson, Haiyan Wang, S.A. Kahn, M. Demarteau, and T. Fahland

Subjects
Physics, Nuclear and High Energy Physics, Particle physics, Large Hadron Collider, 010308 nuclear & particles physics, Supergravity, High Energy Physics::Phenomenology, Tevatron, Electron, Expected value, 7. Clean energy, 01 natural sciences, law.invention, Standard Model, Nuclear physics, law, 0103 physical sciences, High Energy Physics::Experiment, Fermilab, 010306 general physics, Collider
Abstract

We describe a search for evidence of minimal supergravity (MSUGRA) in 92.7 pb(-1) of data collected with the D empty set detector at the Fermilab Tevatron p (p) over bar collider at roots=1.8 TeV. Events with a single electron, four or more jets, and large missing transverse energy were used in this search. The major backgrounds are from W+jets, misidentified multijet, t (t) over bar, and WW production. We observe no excess above the expected number of background events in our data. A new limit in terms of MSUGRA model parameters is obtained.

Published
2002

19. PM266 Predicting 30-years cardiovascular disease in riverside communities in the Brazilian Amazon

Author

Otávio Rizzi Coelho, Diego Jordão Lino Dias, Marcella S. Martello, Manoel G. Souza Neto, Carlos Henrique Spesia, Raitany Costa Almeida, Vinicius R. Grillo, Karime Tuyane Pinheiro Deguchi, and Victor H. Motta

Subjects
Community and Home Care, Epidemiology, Amazon rainforest, business.industry, Environmental health, Medicine, Disease, Cardiology and Cardiovascular Medicine, business
Published
2014

20. Front & Back Matter

Author

Ivana Kholová, Marie Ludvíková, Satoshi Kuwamoto, Aisha Farhat Sheerin, Ralph H. Hruban, Marilyn Davis, Yuki Murai, Anayo L. Ohadugha, Davoud Asgari, Myung Kwan Lim, Luca Mazzucchelli, Makoto Ohta, Julie Weigner, Kennichi Kakudo, Elliot K. Fishman, Yoshihiro Okabe, Ha Young Lee, Cristovam Scapulatempo, Luan Hugolino de Morais, Zahra Maleki, Toshihiko Masago, Yukari Endo, Young Chae Chu, Ibrahim M. Zardawi, Thiraphon Boonyaarunnate, Massimo Bongiovanni, Adhemar Longatto-Filho, Hyunkyung Yoo, Jae Kyun Kim, Pierangela Grassi, Tomoko Honda, Lisa Rahangdale, Naoto Kuroda, Anne Marie Lennon, Yoshiaki Imamura, Paul E. Wakely, Christopher J. VandenBussche, Syed Z. Ali, Fátima Madeira, Monica M.A. Stiepcich, Ramneesh Bhatnagar, Mauro Ajaj Saieg, Laila Tawfik, Tania H. Motta, Satz Mengensatzproduktion, Jae-Yol Lim, Masayuki Shintaku, Debra Budwit, Stephen G. Braye, Momin T. Siddiqui, Young Hye Kang, Anna Novak, Masaki Mori, Takako Kawada, Aadil Ahmed, Cintia Xavier de Mello, Armando de Oliveira Schubach, Fang Fan, Tomoko Wakasa, Hideki Maegawa, Matthew T. Olson, Susan Dillon, Kumiko Inayama, Yasushi Horie, Ossama Tawfik, Valerio A. Vitale, Patrick McElduff, Francisco J. Diaz, Jung Hwan Baek, Werner Druck Medien Ag, Naoyo Ishikura, and Maria E. Fodra

Subjects
Histology, Optics, business.industry, Urology, Pediatrics, Perinatology and Child Health, Medicine, Surgery, Neurology (clinical), General Medicine, Cardiology and Cardiovascular Medicine, business, Pathology and Forensic Medicine, Front (military)
Published
2014

21. Fatigue Life Extension in a Structural Steel

Author

M. A. Carneiro, M. V. Pereira, M. S. Ramos, and S. H. Motta

Subjects
Life extension, Materials science, business.industry, Structural engineering, business
Published
2001

22. Search for dilepton signatures from minimal low-energy supergravity inpp¯collisions ats=1.8TeV

Author

D. Edmunds, Suman Bala Beri, Andre Sznajder, Yuri Gershtein, M. D. Peters, L. Oesch, L. Babukhadia, A. Juste, D. Mihalcea, B. Connolly, Richard Breedon, D. A. Wijngaarden, E. Popkov, E. G. Zverev, Meenakshi Narain, M. Chung, B. Baldin, D. Shpakov, M. Sosebee, T. Heuring, Y. Ducros, P. Demine, H. Zheng, A. N. Galyaev, J. Bantly, Bobby Samir Acharya, H. A. Neal, H. C. Shankar, J. T. Linnemann, H. Motta, X. C. Meng, P. Hanlet, D. Coppage, V. Sorín, K. Yip, Y. Fisyak, Sergey Kuleshov, D. K. Cho, P. A. Rapidis, Elemer Nagy, L. Stutte, Jinhong Yu, A.S. Dyshkant, F. Borcherding, Andrew Brandt, R. Engelmann, R. L. McCarthy, Victor Daniel Elvira, Marvin Johnson, A. Jonckheere, A. P. Vorobiev, Neeti Parashar, U. Bassler, W. E. Cooper, S. Duensing, T. Fahland, Stephan Linn, E. A. Kozlovsky, H.E. Miettinen, S. Youssef, A. V. Kozelov, M. Zielinski, N. Oshima, Daria Zieminska, K. M. Mauritz, B. Olivier, S. Reucroft, R. D. Schamberger, Robert Kehoe, Heriberto Castilla-Valdez, Y. Kulik, Suyong Choi, Miguel Mostafa, P.F. Ermolov, M. Roco, V. Zutshi, G. Eppley, E. Flattum, M. A. Kubantsev, M. M. Baarmand, Sarah Catherine Eno, G. Loreto, J. Womersley, B. May, M. Buehler, Robert Hirosky, J. G.R. Lima, S. Chopra, V. S. Burtovoi, N. R. Stanton, E. J. Ramberg, A. S. Ito, D. P. Stoker, F. Nang, D. Karmanov, Ariel Schwartzman, J. A. Wightman, B. Gibbard, E. Smith, Alberto Santoro, R. Demina, Wendy Taylor, S. Negroni, Richard B. Lipton, A. Patwa, K. Streets, Vipin Bhatnagar, P. Yamin, A. Baden, Brajesh C Choudhary, G. Gutierrez, Seong Keun Kim, D. Toback, Gilvan Alves, G. C. Blazey, R. Jesik, N. I. Bojko, R. D. Martin, R. Partridge, M. Merkin, S. Mani, Yu-tin Huang, D. Denisov, J. Sculli, A. A. Mayorov, Anurag Gupta, R. Yamada, F. Stichelbaut, P. Rubinov, J. M. Hauptman, Cecilia Elena Gerber, Shuichi Kunori, V. Vaniev, R. J. Madaras, R. Snihur, M. Strovink, Srinivasan Rajagopalan, Hal Evans, V. S. Narasimham, Andrew White, P. Gartung, Nikos Varelas, Jasvinder A. Singh, D. Buchholz, Peter W. Draper, Pushpalatha C Bhat, K. Gounder, V. V. Babintsev, G. Ginther, J. Rha, Thomas Ferbel, John Rutherfoord, Raymond Brock, Boaz Klima, R. K. Shivpuri, S. A. Jerger, Jianming Qian, D. Hedin, H. S. Mao, D. R. Claes, Mary Beth Adams, M. A.C. Cummings, B. Hoeneisen, Lee Sawyer, A. Leflat, D. Norman, Anna Goussiou, P. van Gemmeren, J. Snow, S. Doulas, B. Knuteson, M. I. Martin, John Hobbs, S. Willis, G. Briskin, S. N. Gurzhiev, L. Lueking, Haiyan Wang, James C. Green, S. Tentindo-Repond, K. S. Hahn, Kaushik De, S.A. Kahn, T. Rockwell, Iain Alexander Bertram, Jeffrey F. Krane, J. McDonald, M. Demarteau, Gregory R Snow, C. Klopfenstein, D. Cutts, Michael Rijssenbeek, Wagner Carvalho, Greg Landsberg, J. S. Hoftun, K. Davis, J. N. Butler, P. D. Grannis, H. Piekarz, H. L. Melanson, P. Z. Quintas, Philip Baringer, K. Del Signore, S. Banerjee, Viatcheslav Stolin, M. Paterno, K. Soustruznik, V. N. Evdokimov, B. G. Pope, M. H.G. Souza, J. H. Christenson, C. Lundstedt, David H. Adams, Chris Hays, V. A. Bezzubov, J. P. Negret, R. P. Smith, H. E. Montgomery, H. E. Fisk, L. J. Pan, K. M. Chan, Dhiman Chakraborty, Alice Bean, Elizaveta Shabalina, F. Canelli, Michael Shupe, Z. Yu, P. M. Tuts, V. E. Kuznetsov, Z. Zhou, J. Solomon, R. W. Stephens, M. Bhattacharjee, Stephen Wimpenny, J. Estrada, N. Sotnikova, R. J. Genik, T. G. Trippe, J. T. White, B. Pawlik, A. V. Kotwal, Harrison Prosper, S. Desai, Sharon Hagopian, A. V. Kostritskiy, T. Marshall, R. Gilmartin, D. Casey, H. D. Wahl, Sissel Hansen, J. Kotcher, M. Zanabria, Winston Ko, Darien Wood, O. I. Dahl, R. Piegaia, L. Coney, N. Sen, N. Graf, Nicholas John Hadley, S. Blessing, T. Yasuda, Scott Snyder, V. Buescher, E. W. Anderson, Q. Z. Li, Allen Mincer, J. V.D. Wirjawan, Sebastian Grinstein, E. Kajfasz, V. Simak, V. Oguri, P. Padley, Alexey Volkov, James H Cochran, Gustaaf Brooijmans, M. L. Stevenson, J. Warchol, Mitchell Wayne, H. Schellman, J. M. Kohli, Elizabeth Gallas, T. McMahon, Stefan Grünendahl, J. Thompson, S. R. Hou, B. Gómez, K. A. Johns, Z. H. Zhu, Shashikant Dugad, R. Van Kooten, S. Feher, A. Besson, G. Graham, A. Zieminski, R. A. Sidwell, G. Steinbrück, Rajendran Raja, Brad Abbott, C. S. Mishra, Vasken Hagopian, H. Weerts, Z. M. Wang, Lev Dudko, S. Fuess, Vladimir Gavrilov, Gordon Watts, V. Sirotenko, P. I. Goncharov, O. V. Eroshin, Phillip Gutierrez, C. Miao, M. Abolins, D. A. Stoyanova, A. S. Turcot, Gervasio Gomez, N. K. Mondal, V.V. Abramov, H. T. Diehl, H. Singh, Michael Begel, D. Whiteson, A. Boehnlein, Matthew Jones, K. Genser, J. Ellison, A. K.A. Maciel, Arnaud Lucotte, Ulrich Heintz, H. Jöstlein, N. Parua, Randy Ruchti, M. Diesburg, Alexander Belyaev, Patrick Slattery, S. Krzywdzinski, A. Zylberstejn, F. Lehner, H. Greenlee, A. Bross, O. Peters, G. A. Davis, Serban Protopopescu, N.V. Mokhov, A. P. Heinson, R. E. Hall, J. L. González Solís, Z. Casilum, D. Fein, Don Lincoln, P. W. Balm, Daniel John Karmgard, C. Hebert, Jing Li, N. Amos, G. Bernardi, Emanuela Barberis, L. T. Goss, H. Haggerty, M. Fortner, N. W. Reay, J. Perkins, K. W. Merritt, J. F. Bartlett, V. Manankov, F. Fleuret, Howard Gordon, K. C. Frame, S. P. Denisov, P. Grudberg, M. R. Krishnaswamy, A. R. Clark, A. Para, Sergey Chekulaev, and Michael A. Strauss

Subjects
Physics, Nuclear and High Energy Physics, Gluino, Particle physics, 010308 nuclear & particles physics, Supergravity, High Energy Physics::Phenomenology, Supersymmetry, Coupling (probability), 01 natural sciences, Chargino, 0103 physical sciences, Neutralino, High Energy Physics::Experiment, 010306 general physics, Energy (signal processing), Lepton
Abstract

We report on a search for supersymmetry using the D\O{} detector. The 1994--1996 data sample of $\sqrt{s}=1.8$ TeV $p\overline{p}$ collisions was analyzed for events containing two leptons $(e$ or $\ensuremath{\mu}),$ two or more jets, and missing transverse energy. Assuming the minimal supergravity model, with ${A}_{0}=0$ and $\ensuremath{\mu}l0,$ various thresholds were employed to optimize the search. No events were found beyond expectation from the background. We set a lower limit at the 95% C.L. of 255 $\mathrm{GeV}{/c}^{2}$ for equal mass squarks and gluinos for $\mathrm{tan}\ensuremath{\beta}=2,$ and present exclusion contours in the ${(m}_{0}{,m}_{1/2})$ plane for $\mathrm{tan}\ensuremath{\beta}=2$--6.

Published
2001

23. Measurement of the angular distribution of electrons fromW→eνdecays observed inpp¯collisions ats=1.8TeV

Author

S. N. Gurzhiev, J. Womersley, G. Eppley, J. G.R. Lima, M. Abolins, D. Karmanov, A. S. Ito, Viatcheslav Stolin, E. G. Zverev, C. Lundstedt, F. Stichelbaut, J. A. Wightman, T. Fahland, N. K. Mondal, L. Lueking, G. Loreto, H. T. Diehl, H. Singh, A. Jonckheere, Suyong Choi, J. Krane, Neeti Parashar, A. V. Kostritskiy, B. May, A. P. Vorobiev, Stephan Linn, M. Zielinski, N. I. Bojko, D. Whiteson, A. Boehnlein, R. D. Martin, Gregorio Bernardi, D. Denisov, T. G. Trippe, K. Del Signore, Srinivasan Rajagopalan, M. Sosebee, Hal Evans, Thomas Ferbel, N. Oshima, J. Ellison, Raymond Brock, Boaz Klima, H. A. Neal, A. K.A. Maciel, Sissel Hansen, H. E. Fisk, S. Fuess, H. C. Shankar, V. Sorín, Daria Zieminska, Sharon Hagopian, T. Rockwell, Iain Alexander Bertram, K. Yip, C. Klopfenstein, L. J. Pan, Michael Rijssenbeek, L. Stutte, Jinhong Yu, E. Flattum, F. Borcherding, J. Bantly, H. Motta, L. Coney, M. A. Kubantsev, A.S. Dyshkant, D. Norman, P. M. Tuts, N. Sen, V. E. Kuznetsov, R. W. Stephens, E. J. Ramberg, Anurag Gupta, S. Willis, S. Chopra, R. D. Schamberger, R. L. McCarthy, A. A. Mayorov, V. N. Evdokimov, Z. Zhou, J. Solomon, S. A. Jerger, J. T. Linnemann, R. Piegaia, M. Strovink, G. Steinbrück, Shuichi Kunori, A. Leflat, Robert Hirosky, K. A. Johns, Andrew Brandt, J. S. Hoftun, K. Davis, J. N. Butler, P. D. Grannis, R. Engelmann, K. Streets, Vipin Bhatnagar, P. Yamin, D. Mihalcea, Richard Breedon, M. I. Martin, Ariel Schwartzman, R. Snihur, V. Buescher, M. Chung, H. Piekarz, R. Demina, V. Vaniev, A. N. Galyaev, M. Roco, G. Gutierrez, Gilvan Alves, R. Partridge, R. Gilmartin, E. Smith, S. Tentindo-Repond, E. W. Anderson, D. Shpakov, J. Rha, T. Heuring, H. S. Mao, M. Bhattacharjee, V. S. Narasimham, L. Groer, J. McDonald, Q. Z. Li, P. Demine, Allen Mincer, E. A. Kozlovsky, Miguel Mostafa, M. M. Baarmand, H. Zheng, Sarah Catherine Eno, P. Hanlet, D. K. Cho, W. E. Cooper, S. Duensing, B. Baldin, J. Snow, U. Bassler, M. Merkin, D. Coppage, Sebastian Grinstein, Andrew White, D. Hedin, H.E. Miettinen, Y. Ducros, V. Simak, J. B. Singh, Philip Baringer, P. Padley, Alexey Volkov, F. Nang, Peter W. Draper, B. Pawlik, Chris Hays, Richard B. Lipton, Heriberto Castilla-Valdez, Elizabeth Gallas, T. McMahon, H. Jostlein, James H Cochran, Mitchell Wayne, V. A. Bezzubov, J. P. Negret, R. P. Smith, D. Casey, H. Schellman, J. M. Kohli, S. Banerjee, S. Negroni, N. Sotnikova, R. J. Genik, J. Kotcher, Y. Fisyak, Michael Shupe, K. M. Chan, Dhiman Chakraborty, Wendy Taylor, N. Amos, A. Patwa, X. C. Meng, K. S. Hahn, O. I. Dahl, Alberto Santoro, Stefan Grünendahl, J. Thompson, D. A. Wijngaarden, S. R. Hou, John Hobbs, Marvin Johnson, D. Cutts, Roger Moore, Y. Kulik, S. Reucroft, Pushpalatha C Bhat, B. Gómez, Z. H. Zhu, H. Haggerty, D. Toback, J. Estrada, K. Gounder, A. V. Kozelov, Sergey Kuleshov, N. Graf, Wagner Carvalho, M. R. Adams, Greg Landsberg, Victor Daniel Elvira, A. V. Kotwal, Harrison Prosper, M. Buehler, P. Rubinov, P. I. Goncharov, S. Desai, P. Gartung, M. A.C. Cummings, B. Hoeneisen, Lee Sawyer, H. L. Melanson, P. Z. Quintas, L. Babukhadia, B. Connolly, J. M. Hauptman, Cecilia Elena Gerber, S. Youssef, N. R. Stanton, M. Fortner, S. K. Kim, J. H. Christenson, B. S. Acharya, M. Paterno, K. Soustruznik, C. Miao, B. Olivier, D. P. Stoker, N. W. Reay, Robert Kehoe, V. V. Babintsev, G. Ginther, V. Zutshi, J. Perkins, David H. Adams, B. Knuteson, Alice Bean, Haiyan Wang, S.A. Kahn, A. Baden, G. C. Blazey, Jianming Qian, M. Demarteau, R. Yamada, R. Jesik, H. D. Wahl, M. Zanabria, Winston Ko, Emanuela Barberis, F. Canelli, John Rutherfoord, L. T. Goss, D. Edmunds, James C. Green, T. Yasuda, G. Briskin, B. G. Pope, Andre Sznajder, Yuri Gershtein, Elizaveta Shabalina, M. D. Peters, L. Oesch, J. T. White, Michael A. Strauss, Suman Bala Beri, A. Juste, Vladimir Gavrilov, Gordon Watts, M. H.G. Souza, V. Sirotenko, O. V. Eroshin, Phillip Gutierrez, V.V. Abramov, Matthew Jones, Arnaud Lucotte, H. E. Montgomery, N. Parua, Randy Ruchti, C. S. Mishra, Vasken Hagopian, H. Weerts, Z. M. Wang, Lev Dudko, Z. Yu, D. Buchholz, J. V.D. Wirjawan, Anna Goussiou, Gregory R Snow, E. Kajfasz, K. M. Mauritz, P.F. Ermolov, V. Oguri, E. Popkov, Meenakshi Narain, V. S. Burtovoi, Gustaaf Brooijmans, M. L. Stevenson, J. Warchol, Stephen Wimpenny, R. J. Madaras, T. Marshall, Darien Wood, Nicholas John Hadley, S. Blessing, Scott Snyder, S. Krzywdzinski, R. K. Shivpuri, D. R. Claes, Alexander Belyaev, P. van Gemmeren, Patrick Slattery, D. A. Stoyanova, A. S. Turcot, Gervasio Gomez, Michael Begel, S. Doulas, A. Zylberstejn, Ulrich Heintz, F. Lehner, H. Greenlee, M. Diesburg, Kaushik De, A. Bross, O. Peters, G. A. Davis, Serban Protopopescu, S. M. Tripathi, B. Gibbard, N.V. Mokhov, Elemer Nagy, Yu-tin Huang, J. Sculli, Nikos Varelas, A. P. Heinson, R. E. Hall, J. L. González Solís, Z. Casilum, D. Fein, Don Lincoln, P. W. Balm, Daniel John Karmgard, C. Hebert, F. Fleuret, S. R. Dugad, Jing Li, Howard Gordon, K. C. Frame, K. W. Merritt, J. F. Bartlett, V. Manankov, S. P. Denisov, P. Grudberg, M. R. Krishnaswamy, A. R. Clark, A. Para, Sergey Chekulaev, R. Van Kooten, S. Feher, A. Besson, G. Graham, A. Zieminski, P. A. Rapidis, R. A. Sidwell, K. Genser, Rajendran Raja, and Brad Abbott

Subjects
Physics, Quantum chromodynamics, Nuclear and High Energy Physics, Particle physics, 010308 nuclear & particles physics, High Energy Physics::Phenomenology, Tevatron, Perturbative QCD, Electron, 01 natural sciences, Nuclear physics, Particle decay, 0103 physical sciences, High Energy Physics::Experiment, Fermilab, Polar coordinate system, 010306 general physics, Parametrization
Abstract

We present the first measurement of the electron angular distribution parameter α 2 in W→eν events produced in proton-antiproton collisions as a function of the W boson transverse momentum. Our analysis is based on data collected using the DO detector during the 1994–1995 Fermilab Tevatron run. We compare our results with next-to-leading order perturbative QCD, which predicts an angular distribution of (1±α 1 cosθ ∗ +α 2 cos 2 θ ∗ ), where θ ∗ is the polar angle of the electron in the Collins-Soper frame. In the presence of QCD corrections, the parameters α 1 and α 2 become functions of p T W , the W boson transverse momentum. This measurement provides a test of next-to-leading order QCD corrections which are a non-negligible contribution to the W boson mass measurement.

Published
2001

27. Effect of fiber-matrix adhesion on the creep behavior of CF/PPS composites: temperature and physical aging characterization

Author

J. W. Luinge, Harald E.N. Bersee, M. H. Motta Dias, Rinze Benedictus, and Kaspar M. B. Jansen

Subjects
viscoelastic creep behavior, Materials science, Sulfide, General Chemical Engineering, physical aging, Aerospace Engineering, 02 engineering and technology, 010402 general chemistry, 01 natural sciences, Article, Isothermal process, Viscoelasticity, Materials Science(all), Ultimate tensile strength, General Materials Science, Time aging–time superposition, Composite material, time superposition, time aging, CF/PPS composites, chemistry.chemical_classification, Mechanical Engineering, time temperature superposition principle (TTSP), Adhesion, Test method, 021001 nanoscience & nanotechnology, 0104 chemical sciences, chemistry, Creep, Solid mechanics, Chemical Engineering(all), 0210 nano-technology
Abstract

The influence of fiber-matrix adhesion on the linear viscoelastic creep behavior of ‘as received’ and ‘surface modified’ carbon fibers (AR-CF and SM-CF, respectively) reinforced polyphenylene sulfide (PPS) composite materials was investigated. Short-term tensile creep tests were performed on ±45° specimens under six different isothermal conditions, 40, 50, 60, 65, 70 and 75 °C. Physical aging effects were evaluated on both systems using the short-term test method established by Struik. The results showed that the shapes of the curves were affected neither by physical aging nor by the test temperature, allowing then superposition to be made. A unified model was proposed with a single physical aging and temperature-dependent shift factor, \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$a_{T,te}$\end{document}aT,te. It was suggested that the surface treatment carried out in SM-CF/PPS had two major effects on the creep response of CF/PPS composites at a reference temperature of 40 °C: a lowering of the initial compliance of about 25 % and a slowing down of the creep response of about 1.1 decade.

Full Text
View/download PDF

30. Comparative microbiome analysis in cystic fibrosis and non-cystic fibrosis bronchiectasis.

Author

Motta H, Reuwsaat JCV, Lopes FC, Viezzer G, Volpato FCZ, Barth AL, de Tarso Roth Dalcin P, Staats CC, Vainstein MH, and Kmetzsch L

Subjects
Humans, Male, Female, Adult, Middle Aged, Sputum microbiology, Young Adult, Cohort Studies, Aged, Bronchiectasis microbiology, Bronchiectasis drug therapy, Bronchiectasis diagnosis, Cystic Fibrosis microbiology, Cystic Fibrosis drug therapy, Cystic Fibrosis diagnosis, Microbiota physiology, Microbiota drug effects
Abstract

Background: Bronchiectasis is a condition characterized by abnormal and irreversible bronchial dilation resulting from lung tissue damage and can be categorized into two main groups: cystic fibrosis (CF) and non-CF bronchiectasis (NCFB). Both diseases are marked by recurrent infections, inflammatory exacerbations, and lung damage. Given that infections are the primary drivers of disease progression, characterization of the respiratory microbiome can shed light on compositional alterations and susceptibility to antimicrobial drugs in these cases compared to healthy individuals., Methods: To assess the microbiota in the two studied diseases, 35 subjects were recruited, comprising 10 NCFB and 13 CF patients and 12 healthy individuals. Nasopharyngeal swabs and induced sputum were collected, and total DNA was extracted. The DNA was then sequenced by the shotgun method and evaluated using the SqueezeMeta pipeline and R., Results: We observed reduced species diversity in both disease cohorts, along with distinct microbial compositions and profiles of antimicrobial resistance genes, compared to healthy individuals. The nasopharynx exhibited a consistent microbiota composition across all cohorts. Enrichment of members of the Burkholderiaceae family and an increased Firmicutes/Bacteroidetes ratio in the CF cohort emerged as key distinguishing factors compared to NCFB group. Staphylococcus aureus and Prevotella shahii also presented differential abundance in the CF and NCFB cohorts, respectively, in the lower respiratory tract. Considering antimicrobial resistance, a high number of genes related to antibiotic efflux were detected in both disease groups, which correlated with the patient's clinical data., Conclusions: Bronchiectasis is associated with reduced microbial diversity and a shift in microbial and resistome composition compared to healthy subjects. Despite some similarities, CF and NCFB present significant differences in microbiome composition and antimicrobial resistance profiles, suggesting the need for customized management strategies for each disease., (© 2024. The Author(s).)

Published
2024
Full Text
View/download PDF

31. The small heat shock protein Hsp12.1 has a major role in the stress response and virulence of Cryptococcus gattii.

Author

Motta H, Catarina Vieira Reuwsaat J, Daidrê Squizani E, da Silva Camargo M, Wichine Acosta Garcia A, Schrank A, Henning Vainstein M, Christian Staats C, and Kmetzsch L

Subjects
Animals, Mice, Phagocytosis, Virulence, Cryptococcosis microbiology, Cryptococcus gattii genetics, Heat-Shock Proteins, Small metabolism
Abstract

Cryptococcus gattii is one of the etiological agents of cryptococcosis. To achieve a successful infection, C. gattii cells must overcome the inhospitable host environment and deal with the highly specialized immune system and poor nutrients availability. Inside the host, C. gattii uses a diversified set of tools to maintain homeostasis and establish infection, such as the expression of remarkable and diverse heat shock proteins (Hsps). Grouped by molecular weight, little is known about the Hsp12 subset in pathogenic fungi. In this study, the function of the C. gattii HSP12.1 and HSP12.2 genes was characterized. Both genes were upregulated during murine infection and heat shock. The hsp12.1 Δ null mutant cells were sensitive to plasma membrane and oxidative stressors. Moreover, HSP12 deletion induced C. gattii reactive oxygen species (ROS) accumulation associated with a differential expression pattern of oxidative stress-responsive genes compared to the wild type strain. Apart from these findings, the deletion of the paralog gene HSP12.2 did not lead to any detectable phenotype. Additionally, the double-deletion mutant strain hsp12.1 Δ /hsp12.2 Δ presented a similar phenotype to the single-deletion mutant hsp12.1 Δ, suggesting a minor participation of Hsp12.2 in these processes. Furthermore, HSP12.1 disruption remarkably affected C. gattii virulence and phagocytosis by macrophages in an invertebrate model of infection, demonstrating its importance for C. gattii pathogenicity., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Inc. All rights reserved.)

Published
2023
Full Text
View/download PDF

32. Polyketides produced by the entomopathogenic fungus Metarhizium anisopliae induce Candida albicans growth.

Author

Sbaraini N, Hu J, Roux I, Phan CS, Motta H, Rezaee H, Schrank A, Chooi YH, and Staats CC

Subjects
Animals, Antifungal Agents, Aspergillus nidulans genetics, Fungi drug effects, Gene Expression Regulation, Fungal, Genome, Fungal genetics, Larva microbiology, Microbial Interactions physiology, Microbial Sensitivity Tests, Moths, Multigene Family, Polyketides chemistry, Polyketides isolation & purification, Secondary Metabolism genetics, Biosynthetic Pathways genetics, Candida albicans drug effects, Candida albicans growth & development, Metarhizium genetics, Metarhizium metabolism, Polyketides metabolism, Polyketides pharmacology
Abstract

Metarhizium anisopliae is an important entomopathogenic species and model for arthropod-fungus interaction studies. This fungus harbors a diverse arsenal of unexplored secondary metabolite biosynthetic gene clusters, which are suggested to perform diverse roles during host interaction and soil subsistence as a saprophytic species. Here we explored an unusual carnitine acyltransferase domain-containing highly reducing polyketide synthase found in the genome of M. anisopliae. Employing heterologous expression in Aspergillus nidulans, two new polyketides were obtained, named BAA and BAB, as well as one known polyketide [(2Z,4E,6E)-octa-2,4,6-trienedioic acid]. Intra-hemocoel injection of the most abundant compound (BAA) in the model-arthropod Galleria mellonella larvae did not induce mortality or noticeable alterations, suggesting that this compound may not harbor insecticidal activity. Also, the potential role of such molecules in polymicrobial interactions was evaluated. Determination of minimum inhibitory concentration assays using distinct fungal species revealed that BAA and BAB did not alter Cryptococcus neoformans growth, while BAA exhibited weak antifungal activity against Saccharomyces cerevisiae. Unexpectedly, these compounds increased Candida albicans growth compared to control conditions. Furthermore, BAA can mitigate the fungicidal effects of fluconazole over C. albicans. Although the exact role of these compounds on the M. anisopliae life cycle is elusive, the described results add up to the complexity of secondary metabolites produced by Metarhizium spp. Moreover, up to our knowledge, these are the first polyketides isolated from filamentous fungi that can boost the growth of another fungal species., (Copyright © 2021 Elsevier Inc. All rights reserved.)

Published
2021
Full Text
View/download PDF

33. New nanotechnological formulation based on amiodarone-loaded lipid core nanocapsules displays anticryptococcal effect.

Author

Oliveira NK, Frank LA, Squizani ED, Reuwsaat JCV, Marques BM, Motta H, Garcia AWA, Kinskovski UP, Barcellos VA, Schrank A, Pohlmann AR, Staats CC, Guterres SS, Vainstein MH, and Kmetzsch L

Subjects
Animals, Antifungal Agents pharmacology, Antifungal Agents therapeutic use, Fluconazole therapeutic use, Lipids therapeutic use, Mice, Microbial Sensitivity Tests, Nanotechnology, Amiodarone, Cryptococcosis drug therapy, Nanocapsules therapeutic use
Abstract

Cryptococcus neoformans is the etiological agent of cryptococcal meningoencephalitis. The recommended available treatment has low efficiency, with high toxicity and resistance as recurrent problems. In the search of new treatment protocols, the proposal of new pharmacological approaches is considered an innovative strategy, mainly nanotechnological systems considering fungal diseases. The antiarrhythmic drug amiodarone has demonstrated antifungal activity against a range of fungi, including C. neoformans. Here, considering the importance of calcium storage mediated by transporters on cryptococcal virulence, we evaluated the use of the calcium channel blocker amiodarone as an alternative therapy for cryptococcosis. C. neoformans displayed high sensitivity to amiodarone, which was also synergistic with fluconazole. Amiodarone treatment influenced some virulence factors, interrupting the calcium-calcineurin signaling pathway. Experiments with murine cryptococcosis models revealed that amiodarone treatment increased the fungal burden in the lungs, while its combination with fluconazole did not improve treatment compared to fluconazole alone. In addition, we have developed different innovative nanotechnological formulations, one of which combining two drugs with different mechanisms of action. Lipid-core nanocapsules (LNC) loaded with amiodarone (LNC AMD ), fluconazole (LNC FLU ) and both (LNC AMD+FLU ) were produced to achieve a better efficacy in vivo. In an intranasal model of treatment, all the LNC formulations had an antifungal effect. In an intraperitoneal treatment, LNC AMD showed an enhanced anticryptococcal effect compared to the free drug, whereas LNC FLU or LNC AMD+FLU displayed no differences from the free drugs. In this way, nanotechnology using amiodarone formulations could be an effective therapy for cryptococcal infections., (Copyright © 2021. Published by Elsevier B.V.)

Published
2021
Full Text
View/download PDF

34. The Transcription Factor Pdr802 Regulates Titan Cell Formation and Pathogenicity of Cryptococcus neoformans.

Author

Reuwsaat JCV, Agustinho DP, Motta H, Chang AL, Brown H, Brent MR, Kmetzsch L, and Doering TL

Subjects
Animals, Female, Fungal Proteins metabolism, Gene Deletion, Giant Cells microbiology, Mice, Mice, Inbred BALB C, Transcription Factors metabolism, Virulence Factors metabolism, Cryptococcus neoformans genetics, Cryptococcus neoformans pathogenicity, Fungal Proteins genetics, Gene Expression Regulation, Fungal genetics, Giant Cells physiology, Host-Pathogen Interactions, Transcription Factors genetics
Abstract

Cryptococcus neoformans is a ubiquitous, opportunistic fungal pathogen that kills almost 200,000 people worldwide each year. It is acquired when mammalian hosts inhale the infectious propagules; these are deposited in the lung and, in the context of immunocompromise, may disseminate to the brain and cause lethal meningoencephalitis. Once inside the host, C. neoformans undergoes a variety of adaptive processes, including secretion of virulence factors, expansion of a polysaccharide capsule that impedes phagocytosis, and the production of giant (Titan) cells. The transcription factor Pdr802 is one regulator of these responses to the host environment. Expression of the corresponding gene is highly induced under host-like conditions in vitro and is critical for C. neoformans dissemination and virulence in a mouse model of infection. Direct targets of Pdr802 include the quorum sensing proteins Pqp1, Opt1, and Liv3; the transcription factors Stb4, Zfc3, and Bzp4, which regulate cryptococcal brain infectivity and capsule thickness; the calcineurin targets Had1 and Crz1, important for cell wall remodeling and C. neoformans virulence; and additional genes related to resistance to host temperature and oxidative stress, and to urease activity. Notably, cryptococci engineered to lack Pdr802 showed a dramatic increase in Titan cells, which are not phagocytosed and have diminished ability to directly cross biological barriers. This explains the limited dissemination of pdr802 mutant cells to the central nervous system and the consequently reduced virulence of this strain. The role of Pdr802 as a negative regulator of Titan cell formation is thus critical for cryptococcal pathogenicity. IMPORTANCE The pathogenic yeast Cryptococcus neoformans presents a worldwide threat to human health, especially in the context of immunocompromise, and current antifungal therapy is hindered by cost, limited availability, and inadequate efficacy. After the infectious particle is inhaled, C. neoformans initiates a complex transcriptional program that integrates cellular responses and enables adaptation to the host lung environment. Here, we describe the role of the transcription factor Pdr802 in the response to host conditions and its impact on C. neoformans virulence. We identified direct targets of Pdr802 and also discovered that it regulates cellular features that influence movement of this pathogen from the lung to the brain, where it causes fatal disease. These findings significantly advance our understanding of a serious disease., (Copyright © 2021 Reuwsaat et al.)

Published
2021
Full Text
View/download PDF

35. Effects of foliar fungicide on yield, micronutrients, and cadmium in grains from historical and modern hard winter wheat genotypes.

Author

Motta-Romero H, Niyongira F, Boehm JD Jr, and Rose DJ

Subjects
Edible Grain metabolism, Genotype, Seasons, Soil Pollutants analysis, Triticum drug effects, Triticum genetics, Triticum metabolism, Cadmium metabolism, Fungicides, Industrial adverse effects, Micronutrients analysis, Minerals analysis, Plant Breeding methods, Triticum growth & development
Abstract

Plant breeding and disease management practices have increased the grain yield of hard winter wheat (Triticum aestivum L.) adapted to the Great Plains of the United States during the last century. However, the effect of genetic gains for seed yield and the application of fungicide on the micronutrient and cadmium (Cd) concentration in wheat grains is still unclear. The objectives of this study were to evaluate the effects of fungicide application on the productivity and nutritional quality of wheat cultivars representing 80 years of plant breeding efforts. Field experiments were conducted over two crop years (2017 and 2018) with eighteen hard winter wheat genotypes released between 1933 and 2013 in the presence or absence of fungicide application. For each growing season, the treatments were arranged in a split-plot design with the fungicide levels (treated and untreated) as the whole plot treatments and the genotypes as split-plot treatments in triplicate. The effects on seed yield, grain protein concentration (GPC), micronutrients, phytic acid, and Cd in grains were measured. While the yield of wheat was found to increase at annualized rates of 26.5 and 13.0 kg ha-1 yr-1 in the presence and absence of fungicide (P < 0.001), respectively, GPC (-190 and -180 mg kg-1 yr-1, P < 0.001), Fe (-35.0 and -44.0 μg kg-1 yr-1, P < 0.05), and Zn (-68.0 and -57.0 μg kg-1 yr-1, P < 0.01) significantly decreased during the period studied. In contrast to the other mineral elements, grain Cd significantly increased over time (0.4 μg kg-1 yr-1, P < 0.01) in the absence of fungicide. The results from this study are of great concern, as many mineral elements essential for human nutrition have decreased over time while the toxic heavy metal, Cd, has increased, indicating modern wheats are becoming a better vector of dietary Cd., Competing Interests: The authors have declared that no competing interests exist.

Published
2021
Full Text
View/download PDF

36. Participation of Zip3, a ZIP domain-containing protein, in stress response and virulence in Cryptococcus gattii.

Author

Garcia AWA, Kinskovski UP, Diehl C, Reuwsaat JCV, Motta de Souza H, Pinto HB, Trentin DDS, de Oliveira HC, Rodrigues ML, Becker EM, Kmetzsch L, Vainstein MH, and Staats CC

Subjects
Animals, Cryptococcosis genetics, Cryptococcosis microbiology, Cryptococcosis pathology, Cryptococcus gattii metabolism, Cryptococcus gattii pathogenicity, Humans, Macrophages metabolism, Manganese metabolism, Phenotype, Reactive Oxygen Species metabolism, Virulence genetics, Cation Transport Proteins genetics, Cryptococcus gattii genetics, Saccharomyces cerevisiae Proteins genetics, Ubiquitin-Protein Ligases genetics
Abstract

Cryptococcus gattii is an etiologic agent of cryptococcosis, a potentially fatal disease that affects humans and animals. The successful infection of mammalian hosts by cryptococcal cells relies on their ability to infect and survive in macrophages. Such phagocytic cells present a hostile environment to intracellular pathogens via the production of reactive nitrogen and oxygen species, as well as low pH and reduced nutrient bioavailability. To overcome the low-metal environment found during infection, fungal pathogens express high-affinity transporters, including members of the ZIP family. Previously, we determined that functional zinc uptake driven by Zip1 and Zip2 is necessary for full C.gattiivirulence. Here, we characterized the ZIP3 gene of C. gattii, an ortholog of the Saccharomyces cerevisiae ATX2, which codes a manganese transporter localized to the membrane of the Golgi apparatus. Cryptococcal cells lacking Zip3 were tolerant to toxic concentrations of manganese and had imbalanced expression of intracellular metal transporters, such as the vacuolar Pmc1 and Vcx1, as well as the Golgi Pmr1. Moreover, null mutants of the ZIP3 gene displayed higher sensitivity to reactive oxygen species (ROS) and substantial alteration in the expression of ROS-detoxifying enzyme-coding genes. In line with these phenotypes, cryptococcal cells displayed decreased virulence in a non-vertebrate model of cryptococcosis. Furthermore, we found that the ZIP3 null mutant strain displayed decreased melanization and secretion of the major capsular component glucuronoxylomannan, as well as an altered extracellular vesicle dimensions profile. Collectively, our data suggest that Zip3 activity impacts the physiology, and consequently, several virulence traits of C. gattii., (Copyright © 2020 Elsevier Inc. All rights reserved.)

Published
2020
Full Text
View/download PDF

37. Calcium Binding Protein Ncs1 Is Calcineurin Regulated in Cryptococcus neoformans and Essential for Cell Division and Virulence.

Author

Squizani ED, Reuwsaat JCV, Lev S, Motta H, Sperotto J, Kaufman-Francis K, Desmarini D, Vainstein MH, Staats CC, Djordjevic JT, and Kmetzsch L

Subjects
Animals, Cryptococcus neoformans chemistry, Female, Fungal Proteins genetics, Gene Expression Regulation, Fungal, Humans, Mice, Mice, Inbred C57BL, Signal Transduction, Virulence genetics, Calcineurin genetics, Calcium-Binding Proteins genetics, Cell Division genetics, Cryptococcus neoformans genetics, Cryptococcus neoformans pathogenicity, Neuronal Calcium-Sensor Proteins genetics, Neuropeptides genetics
Abstract

Intracellular calcium (Ca 2+ ) is crucial for signal transduction in Cryptococcus neoformans , the major cause of fatal fungal meningitis. The calcineurin pathway is the only Ca 2+ -requiring signaling cascade implicated in cryptococcal stress adaptation and virulence, with Ca 2+ binding mediated by the EF-hand domains of the Ca 2+ sensor protein calmodulin. In this study, we identified the cryptococcal ortholog of neuronal calcium sensor 1 (Ncs1) as a member of the EF-hand superfamily. We demonstrated that Ncs1 has a role in Ca 2+ homeostasis under stress and nonstress conditions, as the ncs1Δ mutant is sensitive to a high Ca 2+ concentration and has an elevated basal Ca 2+ level. Furthermore, NCS1 expression is induced by Ca 2+ , with the Ncs1 protein adopting a punctate subcellular distribution. We also demonstrate that, in contrast to the case with Saccharomyces cerevisiae , NCS1 expression in C. neoformans is regulated by the calcineurin pathway via the transcription factor Crz1, as NCS1 expression is reduced by FK506 treatment and CRZ1 deletion. Moreover, the ncs1Δ mutant shares a high temperature and high Ca 2+ sensitivity phenotype with the calcineurin and calmodulin mutants ( cna1 Δ and cam1Δ ), and the NCS1 promoter contains two calcineurin/Crz1-dependent response elements (CDRE1). Ncs1 deficiency coincided with reduced growth, characterized by delayed bud emergence and aberrant cell division, and hypovirulence in a mouse infection model. In summary, our data show that Ncs1 has a significant role as a Ca 2+ sensor in C. neoformans , working with calcineurin to regulate Ca 2+ homeostasis and, consequently, promote fungal growth and virulence. IMPORTANCE Cryptococcus neoformans is the major cause of fungal meningitis in HIV-infected patients. Several studies have highlighted the important contributions of Ca 2+ signaling and homeostasis to the virulence of C. neoformans Here, we identify the cryptococcal ortholog of neuronal calcium sensor 1 (Ncs1) and demonstrate its role in Ca 2+ homeostasis, bud emergence, cell cycle progression, and virulence. We also show that Ncs1 function is regulated by the calcineurin/Crz1 signaling cascade. Our work provides evidence of a link between Ca 2+ homeostasis and cell cycle progression in C. neoformans ., (Copyright © 2020 Squizani et al.)

Published
2020
Full Text
View/download PDF

38. A Predicted Mannoprotein Participates in Cryptococcus gattii Capsular Structure.

Author

Reuwsaat JCV, Motta H, Garcia AWA, Vasconcelos CB, Marques BM, Oliveira NK, Rodrigues J, Ferrareze PAG, Frases S, Lopes W, Barcellos VA, Squizani ED, Horta JA, Schrank A, Rodrigues ML, Staats CC, Vainstein MH, and Kmetzsch L

Subjects
Animals, Cell Line, Cell Wall chemistry, Cryptococcosis immunology, Cryptococcus gattii genetics, Cryptococcus gattii pathogenicity, Female, Fungal Proteins genetics, Macrophages immunology, Membrane Glycoproteins genetics, Mice, Mutation, Phagocytosis, Phenotype, Polysaccharides genetics, Virulence, Virulence Factors genetics, Cryptococcus gattii chemistry, Fungal Capsules chemistry, Fungal Proteins chemistry, Membrane Glycoproteins chemistry, Polysaccharides chemistry, Virulence Factors chemistry
Abstract

The yeast-like pathogen Cryptococcus gattii is an etiological agent of cryptococcosis. The major cryptococcal virulence factor is the polysaccharide capsule, which is composed of glucuronoxylomannan (GXM), galactoxylomannan (GalXM), and mannoproteins (MPs). The GXM and GalXM polysaccharides have been extensively characterized; however, there is little information about the role of mannoproteins in capsule assembly and their participation in yeast pathogenicity. The present study characterized the function of a predicted mannoprotein from C. gattii , designated Krp1. Loss-of-function and gain-of-function mutants were generated, and phenotypes associated with the capsular architecture were evaluated. The null mutant cells were more sensitive to a cell wall stressor that disrupts beta-glucan synthesis. Also, these cells displayed increased GXM release to the culture supernatant than the wild-type strain did. The loss of Krp1 influenced cell-associated cryptococcal polysaccharide thickness and phagocytosis by J774.A1 macrophages in the early hours of interaction, but no difference in virulence in a murine model of cryptococcosis was observed. In addition, recombinant Krp1 was antigenic and differentially recognized by serum from an individual with cryptococcosis, but not with serum from an individual with candidiasis. Taken together, these results indicate that C. gattii Krp1 is important for the cell wall structure, thereby influencing capsule assembly, but is not essential for virulence in vivo IMPORTANCE Cryptococcus gattii has the ability to escape from the host's immune system through poorly understood mechanisms and can lead to the death of healthy individuals. The role of mannoproteins in C. gattii pathogenicity is not completely understood. The present work characterized a protein, Kpr1, that is essential for the maintenance of C. gattii main virulence factor, the polysaccharide capsule. Our data contribute to the understanding of the role of Kpr1 in capsule structuring, mainly by modulating the distribution of glucans in C. gattii cell wall., (Copyright © 2018 Reuwsaat et al.)

Published
2018
Full Text
View/download PDF

39. Isolation of Egg Yolk Granules as Low-Cholesterol Emulsifying Agent in Mayonnaise.

Author

Motta-Romero H, Zhang Z, Tien Nguyen A, Schlegel V, and Zhang Y

Subjects
Centrifugation, Cholesterol analysis, Cholesterol, Dietary, Food, Viscosity, Egg Yolk chemistry, Emulsifying Agents, Emulsions chemistry, Food Handling methods
Abstract

Egg yolk is an essential ingredient for many food products due to its excellent functional properties such as emulsification. However, the consumers' concern of its cholesterol level has led food industry to seek solutions for its replacement. Utilization of low-fat egg yolk granules as emulsifier can be an alternative strategy. In this study, granules with low cholesterol content were separated from egg yolk by a simple method under easily scalable centrifugal conditions. The egg yolk granules isolated within 0.17 M NaCl solution achieved a 22.5% yield that was similar to that using higher centrifugation speeds and longer time. The yield of egg yolk granules increased from 25% to 32% when the yolk:water ratio was changed from 1:1 to 1:2. Mayonnaise was prepared to evaluate the emulsifying capacity of the egg yolk granules. It was found that egg yolk granules exhibited similar emulsifying activity as that of whole egg yolk, but a better emulsion stabilizing property that is evidenced by the higher viscosity of mayonnaise prepared with the granules. In addition, the viscosities of mayonnaise prepared by spray dried yolk and granules were slightly higher than the liquid counterpart, showing a further improvement by spray drying on the emulsion stabilizing properties. The mechanical spectra of mayonnaise samples by frequency sweep also suggested that granules favored the formation of stronger 3-dimensional arrangements of oil droplets and therefore a more stable emulsion. Results suggest that granules isolated within 0.17 M NaCl solution present best emulsifying properties and can be applied as whole yolk replacer in food emulsions., (© 2017 Institute of Food Technologists®.)

Published
2017
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results