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To investigate the effects of miR-32-5p on the biological behaviors of cervical cancer (CCa), the relevant mechanism was studied in CCa cell lines (HeLa) in vitro.The expression level of miR-32-5p was detected by quantitative reverse transcription polymerase chain reaction (qRT-PCR). TargetScan, miRDB, microRNA databases and Luciferase method were conducted to predict and validate the target gene of miR-32-5p; the effects of miR-32-5p on cell proliferation, clone formation, invasion and migration capacity were analyzed in vitro study.We found miR-32-5p to be significantly inhibited in CCa tissues and cells. Bioinformatics approach together with Luciferase method screened Homeobox B8 (HOXB8) as a downstream regulatory target of miR-32-5p. Besides, HOXB8 was incredibly high expression in CCa tissues and cells. After transfection in HeLa cells by miR-32-5p mimics, HOXB8 expression was indicated to be negatively correlated with miR-32-5p both in qRT-PCR and Western blot (WB) assays. The subsequent experiments showed that decreased expression of HOXB8 resulting from up-regulation of miR-32-5p could weaken the cell proliferation, clone formation, invasion and migration ability of HeLa cells.MiR-32-5p could inhibit the cellular malignant behavior through regulating the expression of HOXB8 in HeLa cells. We provide a new clue for the study of molecular mechanisms of CCa. MiR-32-5p/HOXB8 axis might serve as potential target for the clinical diagnosis and treatment of CCa.

Objective To study the structure and differences of bacterial communities in different soils, and to explore the effectiveness of 16S rRNA sequencing in identification of different soil. Methods Soil samples from 7 places in Shanghai were collected, then bacterial genomic DNA were extracted from them. The fragments of hypervariable region from 16S rRNA sequences were sequenced with high-throughput sequencing techniques. The results were quantified or visualized with bioinformatics software. The differences in diversity and abundance among the three kinds of bacterial communities in soil samples from grassland, forests and beaches were compared and analyzed. Results The statistical differences that existed among the alpha diversity indexes of bacterial communities in soil samples of grassland, forests and beaches had statistical significance. The relative abundance and diversity of bacterial communities in these three kinds of soil were significantly different. Grassland soil had higher Acidobacteria abundance, forest soil had higher Proteobacteria abundance, beach soil had higher Actinobacteria abundance. However, the differences in soil bacterial communities in artificial grasslands, natural grasslands and industrial district grasslands did not have statistical significance. Conclusion 16S rRNA sequencing can effectively distinguish different soils. This method may be able to provide clues for first crime scene inference in criminal cases.基于16S rRNA基因序列对不同土壤细菌群落的差异性分析.目的 研究不同土壤细菌群落的结构和差异，探索16S rRNA基因测序技术在不同土壤鉴定中的有效性。 方法 采集上海市7个地区的土壤样本，提取土壤细菌基因组DNA，采用高通量测序技术对16S rRNA基因序列高变区片段进行测序，测序结果通过生物信息学软件量化或可视化后，比较分析草地、树林和沙滩3类土壤样本间细菌群落多样性和丰度的差异。 结果 草地、树林和沙滩土壤样本细菌群落的alpha多样性指数差异具有统计学意义，三类土壤细菌群落的物种相对丰度和多样性差异明显，草地土壤中酸杆菌门相对丰度较高，树林土壤中变形菌门相对丰度较高，沙滩土壤中放线菌门相对丰度较高。但人工草地、天然草地和工业区草地土壤细菌群落差异无统计学意义。 结论 基于16S rRNA基因序列测序可以有效区分不同类别土壤，该方法有望为刑事案件第一犯罪现场判读提供线索。.法医病理学；法医遗传学；土壤微生物学；DNA，细菌；RNA，核糖体，16S；生物多样性；高通量测序.

There are many factors that need to be considered in the operation sequencing, and there are mutual relations among them, so it has become a hot topic worthy of study. In this paper, based on the parallel consideration of process constraint and minimum manufacturing cost, the concepts of workingsteps sequence matrix and process constraint matrix are proposed. The process constraint matrix is used to ensure that the workingsteps sequences conform to the precedence relation constraint, and the genetic algorithm is used to ensure the minimum manufacturing cost. Finally, the optimal solution of the workingsteps sequence is obtained. The connecting rod of marine diesel engine is taken as an example to verify.

Forensic microorganism is one of the hotspots of forensic science research. Due to its conservatism and specificity, the 16S rRNA gene is found to be an ideal marker for forensic identification. With the rapid development of high throughput sequencing technology, the research on microorganisms has been gradually applied to many fields such as environment and health care. In the field of forensic science, the results of forensic microbiology research, represented by 16S rRNA gene sequencing, are also gradually applied to forensic practice, such as biological samples identification, individual identification, postmortem interval estimation, and regional inference, which not only provide clues for the investigation of cases but also complement and assist traditional methods. This paper describes the research methods and related sequencing technologies of 16S rRNA gene sequencing, summarizes its research progress, and discusses the application value and potential of 16S rRNA in forensic science.16S rRNA基因测序在法医学中的研究进展.法医微生物是目前法医学研究的前沿热点之一。16S rRNA基因由于其保守性与特异性并存的特点，是法医学鉴定的理想标记。伴随着高通量测序技术的快速发展，对微生物的研究已逐步应用于环境、医疗等多个领域。在法医学领域，以16S rRNA基因测序为代表的法医微生物研究成果也逐步应用于法医学实践中的生物检材鉴定、个体识别、死亡时间推断、地域推断等方面，为案件的侦查提供线索，为传统方法作为补充和辅助。本文阐述了16S rRNA基因测序应用于法医学领域的研究方法和相关测序技术，综述了其在法医学领域的研究进展，探讨了16S rRNA在法医学中的应用价值和潜力。.法医遗传学；RNA，核糖体，16S；遗传学，微生物；综述.

Background To investigate the effects of different target plasma remifentanil concentrations on the minimum alveolar concentration of sevoflurane (MAC) for blocking adrenergic response (BAR) during laparoscopic gynaecological surgery with carbon dioxide insufflation. Methods Seventy-five gynaecological patients with ASA I–II undergoing laparoscopic surgery were randomly assigned to three groups. Anaesthesia was induced by sevoflurane, and 0.1 mg kg−1 of vecuronium i.v. was injected to facilitate tracheal intubation. After intubation the target plasma concentrations of remifentanil in Groups 1, 2, and 3 were adjusted to 0, 1, and 2 ng ml−1, respectively. The changes in haemodynamics were observed before and after the creation of carbon dioxide pneumoperitoneum. The MAC BAR of sevoflurane in each group was determined by using an up-and-down sequential-allocation technique, and blood samples were collected at corresponding time points to determine the concentrations of remifentanil, norepinephrine, and epinephrine. Results In Groups 1, 2 and 3, the MAC BAR of sevoflurane was 4.6% (CI 95%: 4.3–4.9%), 2.4% (CI 95%: 2.2–2.6%), and 1.7% (CI 95%: 1.4–2.1%), respectively. No significant differences were found in the increase of norepinephrine, epinephrine, and mean arterial pressure after compared with before insufflation of pneumoperitoneum among the three groups. Conclusions Remifentanil can effectively decrease the sevoflurane concentration to block sympathetic adrenergic response to CO2 pneumoperitoneum stimulus. At similar MAC BAR the haemodynamic and adrenergic response is not affected by the infused remifentanil concentration. Clinical trial registration The number of this clinical trial registry is ChiCTR-TRC-13004005, and the Universal Trial Number is U1111-1151-5630.

To analyse the efficiency of EX16+10Y kit on the forensic detection of the Uygur in Xinjiang province.The blood samples were extracted from 4 620 male individuals of Uygur in Xinjiang province, and amplified by EX16+10Y kit. The typing of amplification products was performed by 3130The genotyping graphs of 15 autosomal STR loci and 10 Y-chromosomal STR loci from 4 620 male individuals of Uygur in Xinjiang province were acquired completely. The genotype distribution of 15 autosomal STR loci was consistent with Hardy-Weinberg equilibrium. The heterozygosity, polymorphism information content and discrimination power of STR loci were 0.637-0.838, 0.580-0.860 and 0.811-0.978, respectively. There were 766 haplotypes in 10 Y -chromosomal STR loci.The test results of EX16+10Y kit is accurate and trustworthy, which can simultaneously be used for the individual identification and the screening of paternal pedigree in practical work.

To establish a species identification system based on DNA genetic markers for plant evidence.Two hundred common plants in Shanghai were collected and identified by morphological characteristics. The primers of gene segmentsThe success rate of amplification was in order ofThe identification system with the combination of

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To investigate the specific microbial signatures in vaginal fluid.Vaginal fluid （16 samples）, saliva （16 samples）, feces （16 samples）, semen （8 samples）, peripheral blood （8 samples）, urine （5 samples）, and nasal secretion （4 samples） were collected respectively. TheThe detected number ofThere is a potential application value to detect

Atmospheric particle number size distributions (size range 0.003–10 μm) were measured between March 2008 and August 2009 at Shangdianzi (SDZ), a rural research station in the North China Plain. These measurements were made in an attempt to better characterize the tropospheric background aerosol in Northern China. The mean particle number concentrations of the total particle, as well as the nucleation, Aitken, accumulation and coarse mode were determined to be 1.2 ± 0.9 × 104, 3.6 ± 7.9 × 103, 4.4 ± 3.4 × 103, 3.5 ± 2.8 × 103 and 2 ± 3 cm−3, respectively. A general finding was that the particle number concentration was higher during spring compared to the other seasons. The air mass origin had an important effect on the particle number concentration and new particle formation events. Air masses from northwest (i.e. inner Asia) favored the new particle formation events, while air masses from southeast showed the highest particle mass concentration. Significant diurnal variations in particle number were observed, which could be linked to new particle formation events, i.e. gas-to-particle conversion. During particle formation events, the number concentration of the nucleation mode rose up to maximum value of 104 cm−3. New particle formation events were observed on 36% of the effective measurement days. The formation rate ranged from 0.7 to 72.7 cm−3 s−1, with a mean value of 8.0 cm−3 s−1. The value of the nucleation mode growth rate was in the range of 0.3–14.5 nm h−1, with a mean value of 4.3 nm h−1. It was an essential observation that on many occasions the nucleation mode was able to grow into the size of cloud condensation nuclei (CCN) within a matter of several hours. Furthermore, the new particle formation was regularly followed by a measurable increase in particle mass concentration and extinction coefficient, indicative of a high abundance of condensable vapors in the atmosphere under study.

Source identification of human biological materials in crime scene plays an important role in reconstructing the crime process. Searching specific genetic markers to identify the source of different human biological materials is the emphasis and difficulty of the research work of legal medical experts in recent years. This paper reviews the genetic markers which are used for identifying the source of human biological materials and studied widely, such as DNA methylation, mRNA, microRNA, microflora and protein, etc. By comparing the principles and methods of source identification of human biological materials using different kinds of genetic markers, different source of human biological material owns suitable marker types and can be identified by detecting single genetic marker or combined multiple genetic markers. Though there is no uniform standard and method for identifying the source of human biological materials in forensic laboratories at present, the research and development of a series of mature and reliable methods for distinguishing different human biological materials play the role as forensic evidence which will be the future development direction.

We study wave propagation in a piezoelectric ceramic half-space with a thin semiconductor film and an air gap between the film and the half-space. Two-dimensional equations for a thin film are used to model the semiconductor film and the air gap. The half-space is governed by the three-dimensional equations of linear piezoelectricity. It is shown that an anti-plane wave can propagate in such a system. An equation that determines the dispersion relation of the wave is obtained. Solutions to the equation show that the wave has both dispersion and attenuation, and can be amplified by a biasing dc electric field.

We study the propagation of anti-plane surface waves in a piezoelectric ceramic half-space carrying a thin layer of a semiconducting material. Two-dimensional equations for the extensional motion of a thin membrane of piezoelectric semiconductors are derived to model the semiconductor layer. A surface wave solution is obtained.

The model amyloid peptide AAKLVFF was expressed as a His-tagged fusion protein with the immunoglobulin-binding domain B1 of streptococcal\ud protein G (GB1), a small (56 residues), stable, single-domain protein. It is shown that expression of this model amyloid peptide is possible and is\ud not hindered by aggregation. Formylation side reactions during the CNBr cleavage are investigated via synthesis of selectively formylated peptides.

The activity of nucleolar organizer regions (NOR) in the peripheral lymphocytes from patients with lung, gastric, intestinal or breast carcinoma was studied with a combined method of Ag-staining and G-band. The frequencies of Ag-NOR were higher in chromosome 15 and sum total from patients with lung cancer, lower in chromosome 14 from patients with breast cancer, and lower in chromosome 14 but higher in chromosome 22 from patients with gastric cancer, as compared with those of controls. No significant variation of the frequencies of Ag-NOR was found in the patients with intestinal cancer. The results indicate that a dominant Ag-stained NOR model is shown in the patients with different carcinoma, i.e., the active expression of rRNA genes may present a speciality concerning the location of carcinoma.