222 results on '"H Sattler"'
Search Results
2. PNS175 GAMIFICATION OF PHARMACOECONOMICS FOR PHARMACY STUDENTS
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A. Edwards, H. Sattler, J. Oestreich, and R. Hendershot
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Pharmacoeconomics ,Medical education ,business.industry ,Health Policy ,Public Health, Environmental and Occupational Health ,Medicine ,Pharmacy ,business - Published
- 2020
3. Deracemisation of profenol core by combining laccase/TEMPO-mediated oxidation and alcohol dehydrogenase-catalysed dynamic kinetic resolution
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Johann H. Sattler, Alba Diaz-Rodriguez, Vicente Gotor, Vicente Gotor-Fernández, Nicolás Ríos-Lombardía, Iván Lavandera, and Wolfgang Kroutil
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Laccase ,chemistry.chemical_classification ,biology ,Chemistry ,Biocatalysis ,biology.protein ,Enantioselective synthesis ,Organic chemistry ,Aldehyde ,Catalysis ,Alcohol dehydrogenase ,Kinetic resolution - Abstract
A mild one-pot methodology has been developed to deracemise rac-2-phenyl-1-propanol by combining the use of non-selective laccase/TEMPO-mediated oxidation with enantioselective bioreduction of the racemic aldehyde intermediate under dynamic conditions. The process was easily scalable and stereocontrollable by selecting the suitable biocatalyst.
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- 2015
4. Introducing an In Situ Capping Strategy in Systems Biocatalysis To Access 6-Aminohexanoic acid
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Philip Engel, Francesco G. Mutti, John M. Woodley, Johann H. Sattler, Jan Pfeffer, Michael Fuchs, Barbara Grischek, and Wolfgang Kroutil
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chemistry.chemical_classification ,Molecular Structure ,Chemistry ,Carboxylic acid ,Cyclohexanol ,Esterases ,General Medicine ,General Chemistry ,Cyclohexanols ,Catalysis ,Enzyme catalysis ,chemistry.chemical_compound ,Ammonia ,Monomer ,Nucleophile ,Liver ,Biocatalysis ,Aminocaproic Acid ,Organic chemistry ,Animals ,Methanol ,Horses - Abstract
The combination of two cofactor self-sufficient biocatalytic cascade modules allowed the successful transformation of cyclohexanol into the nylon-6 monomer 6-aminohexanoic acid at the expense of only oxygen and ammonia. A hitherto unprecedented carboxylic acid capping strategy was introduced to minimize the formation of the dead-end intermediate 6-hydroxyhexanoic acid. For this purpose, the precursor e-caprolactone was converted in aqueous medium in the presence of methanol into the corresponding methyl ester instead of the acid. Hence, it was shown for the first time that esterases--specifically horse liver esterase--can perform the selective ring-opening of e-caprolactone with a clear preference for methanol over water as the nucleophile.
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- 2014
5. Speicheldrüsensonografie in der Differentialdiagnostik von Patienten mit Siccasymptomatik
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H Sattler, Raoul Bergner, Christian Löffler, and Daniel Wadsack
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- 2017
6. FRI0294 Ultrasound of the salivary glands helps to distinguish between primary and secondary sjÖgren syndrome
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H Sattler, Raoul Bergner, Daniel Wadsack, and Christian Löffler
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musculoskeletal diseases ,030203 arthritis & rheumatology ,medicine.medical_specialty ,Pathology ,Secondary Sjögren Syndrome ,Salivary gland ,business.industry ,Ultrasound ,Gastroenterology ,eye diseases ,Rheumatology ,SSS ,stomatognathic diseases ,03 medical and health sciences ,0302 clinical medicine ,medicine.anatomical_structure ,stomatognathic system ,Sicca symptoms ,Internal medicine ,Major Salivary Gland ,medicine ,In patient ,030212 general & internal medicine ,business - Abstract
Background Previous studies have demonstrated typical findings in the ultrasound of salivary glands (USG) in patients with primary Sjogren syndrome (pSS) compared with healthy controls. However, it is unknown, if these findings are only seen in patients with pSS but also in patients with secondary Sjogren syndrome (sSS) or other connective tissue diseases with positive Ro-SSA/La-SSB antibodies. Methods We used an ultrasound score developed by Zhang [1] to investigate salivary glands with a score ranging from 0–48. We compared the score from patients with pSS according the criteria of the American-European Consensus Group (group 1) with patients who fulfilled the clinical criteria (1–4), but were Ro-SSA/La-SSB negative (group 2), with patients who had another rheumatic disease, but had sicca symptoms and were Ro-SSA and/or La-SSB positive (sSS) (group 3), with patients with other rheumatic disease without Ro-SSA or La-SSB antibodies (group 4), with patients with other rheumatic disease with Ro-SSA antibodies but no sicca symptoms (group 5) and with patients with no rheumatic disease (group 6), respectively. We investigated the parotid and the submandibular salivary glands bilaterally. The USG was assessed with a score from 0–48 points with a maximum of 12 points for 4 items each (hyoechoic areas, hyperechoic reflexes, inhomogeneity and distinct organ border). If available the score was correlated with the scintigraphically measured function of the salivary glands. Results We included USG of 92 patients in our study. Group 1 (n=33) had a score of 16.6±11.6; group 2 (n=7) 2.4±3.5; group 3 (n=16) 8.6±9.5; group 4 (n=16) 5.3±7.8; group 5 (n=11) 4.5±6.7 and group 6 (n=9) 1.5±2.3, respectively. The score between group 1 and all other groups was significantly different (p Conclusions USG showed significantly higher scores in patients with pSS, than in patients with sSS or other rheumatic disease. USG as a non invasive investigation might be similarly helpful for the diagnosis of pSS like salivary gland functional tests. USG findings can reliable distinguish between pSS and sSS associated with other rheumatic disease, also if they are positive for Ro-SSA/La-SSB. References Zhang X, Zhang S, He J, Hu F, Liu H, Li J, Zhu J, Li Z. Ultrasonographic evaluation of major salivary glands in primary Sjogren9s syndrome: comparison of two scoring systems. Rheumatology (Oxford). 2015 Sep;54(9):1680–7. Disclosure of Interest None declared
- Published
- 2017
7. Deracemisierung durch simultane bio-oxidative Racematspaltung und Stereoinversion
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Joerg H. Schrittwieser, Eva-Maria Fischereder, Nicholas J. Turner, Peter Macheroux, Wolfgang Kroutil, Elisabeth Fuchs, Bas Groenendaal, Diego Ghislieri, Barbara Grischek, Silvia Wallner, Johann H. Sattler, and Verena Resch
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General Medicine - Abstract
Deracemisierung, also die Umwandlung eines Racemats in ein enantiomerenreines Produkt mit theoretisch 100 % Ausbeute und 100 % ee, stellt eine attraktive, aber auch anspruchsvolle Option fur die asymmetrische Synthese dar. Hier beschreiben wir ein neuartiges Konzept einer chemo-enzymatischen Deracemisierung mittels einer Kaskadenreaktion. Die Reaktionsfolge beinhaltet zwei enantioselektive Oxidationsschritte und einen nicht stereoselektiven Reduktionsschritt, durch welche zur gleichen Zeit eine Stereoinversion und eine kinetische Racematspaltung realisiert werden. Dieses Konzept wird anhand der Umwandlung von rac-Benzylisochinolinen in optisch reine (S)-Berbine vorgestellt. Die racemischen Substrate wurden in enantiomerenreine Produkte (ee>97 %) umgewandelt, wobei Umsatze von bis zu 98 % und Ausbeuten von bis zu 88 % erreicht wurden.
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- 2014
8. Enzymatic asymmetric synthesis of the silodosin amine intermediate
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Johann H. Sattler, Ferdinand Zepeck, Nina Richter, Wolfgang Kroutil, Judith E. Farnberger, Christine S. Fuchs, and Robert C. Simon
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chemistry.chemical_classification ,Reaction conditions ,Organic Chemistry ,Enantioselective synthesis ,Silodosin ,Reductive amination ,Catalysis ,Kinetic resolution ,Inorganic Chemistry ,Enzyme ,chemistry ,Yield (chemistry) ,medicine ,Organic chemistry ,Amine gas treating ,Physical and Theoretical Chemistry ,medicine.drug - Abstract
Various enantiocomplementary ω-transaminases (ωTAs) were investigated in kinetic resolution and asymmetric reductive amination reactions to prepare silodosin amine. Whilst the enzymatic kinetic resolution gave moderate to good results with respect to the yield and enantioselectivity, the asymmetric reductive amination proved to be superior. The best results were obtained with the ωTA originating from (R)-Arthrobacter sp. which afforded the desired bioactive (R)-enantiomer in enantiomerically pure form (ee >97%) at excellent conversion (conv. >97%) under mild and benign reaction conditions.
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- 2014
9. Deracemisation of benzylisoquinoline alkaloids employing monoamine oxidase variants
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Verena Resch, Simon C. Willies, Bas Groenendaal, Barbara Grischek, Johann H. Sattler, Joerg H. Schrittwieser, Diego Ghislieri, Ian Rowles, Wolf-Dieter Lienhart, Nicholas J. Turner, Wolfgang Kroutil, and Eva-Maria Fischereder
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Reticuline ,Steric effects ,chemistry.chemical_compound ,chemistry ,Docking (molecular) ,Stereochemistry ,Monoamine oxidase ,Enantioselective synthesis ,Iminium ,Enantiomeric excess ,Benzylisoquinoline ,Catalysis - Abstract
Chemo-enzymatic deracemisation was applied to obtain the (S)-enantiomer of 1-benzylisoquinolines from the racemate in high isolated yield (up to 85%) and excellent optical purity (ee > 97%). The one-pot deracemisation protocol encompassed enantioselective oxidation by a monoamine oxidase (MAO-N) and concomitant reduction of the resulting iminium species by ammonia-borane. The challenge was the oxidation at the sterically demanding chiral centre. Recently developed variants of MAO-N, featuring an enlarged active-site pocket, turned out to be suitable biocatalysts for these substrates. In contrast to previous MAO-N variants, which preferentially converted the (S)-enantiomer, the MAO-N variant D11 used in the present study was found to oxidise all tested benzylisoquinoline substrates with (R)-enantiopreference. The structural determinants of enantioselectivity were investigated by means of protein–ligand docking simulations. The applicability of the deracemisation system was demonstrated on preparative scale (150 mg) for three benzylisoquinoline alkaloids (natural as well as non-natural), including the hypotensive and antispasmodic agent (S)-reticuline.
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- 2014
10. Asymmetric Amination of Tetralone and Chromanone Derivatives Employing ω-Transaminases
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Wolfgang Kroutil, Tanja Knaus, Desiree Pressnitz, Johann H. Sattler, Francesco G. Mutti, Peter Macheroux, and Christine S. Fuchs
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010405 organic chemistry ,Enantioselective synthesis ,General Chemistry ,010402 general chemistry ,01 natural sciences ,Catalysis ,0104 chemical sciences ,chemistry.chemical_compound ,Enantiopure drug ,chemistry ,Biocatalysis ,Yield (chemistry) ,Tetralone ,Organic chemistry ,Amination - Abstract
Various (S)-selective and (R)-selective ω-transaminases were investigated for the amination of 1- and 2-tetralone and derivatives as well as of 3- and 4-chromanone. All ketones tested were aminated to give the corresponding enantiopure amines (ee > 99%) employing at least one of the enzymes investigated. In most of the cases the (S)- as well as the (R)-enantiomer was obtained in optically pure form. The amination of 3-chromanone was performed on a 100 mg scale leading to optically pure (R)-3-aminochromane (ee > 99%) with complete conversion and 78% isolated yield.
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- 2013
11. Artificial Multi-Enzyme Networks for the Asymmetric Amination ofsec-Alcohols
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Johann H. Sattler, Katharina Tauber, Thomas Dr. Haas, Wolfgang Kroutil, Dominik Koszelewski, Kurt Faber, Jan Pfeffer, Michael Fuchs, Julia Pitzer, and Desiree Pressnitz
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Ketone ,Alcohol ,Stereoisomerism ,010402 general chemistry ,01 natural sciences ,Catalysis ,chemistry.chemical_compound ,Organic chemistry ,Amines ,Amination ,Alcohol dehydrogenase ,chemistry.chemical_classification ,Primary (chemistry) ,biology ,010405 organic chemistry ,Hydride ,Organic Chemistry ,General Chemistry ,0104 chemical sciences ,chemistry ,Alcohols ,biology.protein ,Amine gas treating ,Oxidation-Reduction - Abstract
Various artificial network designs that involve biocatalysts were tested for the asymmetric amination of sec-alcohols to the corresponding α-chiral primary amines. The artificial systems tested involved three to five redox enzymes and were exemplary of a range of different sec-alcohol substrates. Alcohols were oxidised to the corresponding ketone by an alcohol dehydrogenase. The ketones were subsequently aminated by employing a ω-transaminase. Of special interest were redox-neutral designs in which the hydride abstracted in the oxidation step was reused in the amination step of the cascade. Under optimised conditions up to 91 % conversion of an alcohol to the amine was achieved.
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- 2013
- Full Text
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12. Grundzüge eines Vorschlags zur Erweiterung des KDCS-TAS-Kerns für die verteilte Transaktionsverarbeitung.
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Jörg Gonschorek and H. Sattler
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- 1986
13. Verschiedene Möglichkeiten zur Simulation von Druckstoß-Vorgängen in Wasserkraftanlagen.
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Karl Heinz Fasol and H. Sattler
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- 1984
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14. Imaging remission by musculoskeletal ultrasound leads to a better functional outcome – results of the us 7-score implementation study in early rheumatoid arthritis
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I. Fischer, Sarah Ohrndorf, H. Sattler, Anne-Marie Glimm, G. Schmittat, J. Strunk, G.-R. Burmester, Wolfgang A. Schmidt, H. Kellner, Marina Backhaus, and Wolfgang Hartung
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medicine.medical_specialty ,business.industry ,Physical therapy ,Medicine ,Radiology, Nuclear Medicine and imaging ,Musculoskeletal ultrasound ,Early rheumatoid arthritis ,business ,Outcome (game theory) - Published
- 2016
15. [Arthrosonography]
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H, Sattler
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Diagnosis, Differential ,Evidence-Based Medicine ,Rheumatic Diseases ,Humans ,Joints ,Image Enhancement ,Endoscopic Ultrasound-Guided Fine Needle Aspiration ,Ultrasonography - Published
- 2016
16. Duplex sonography of the mesenteric vessels--a critical evaluation of inter-observer variability
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C. F. Dietrich, H. Weiss, Andreas Schuler, G. Schuessler, H. Sattler, Christian Jenssen, N. Boerner, A. Bruening, A von Herbay, K. Dirks, R. Kubale, and A. Ignee
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Adult ,Male ,medicine.medical_specialty ,Sensitivity and Specificity ,Ischemic colitis ,030218 nuclear medicine & medical imaging ,03 medical and health sciences ,Young Adult ,0302 clinical medicine ,Reference Values ,medicine.artery ,medicine ,Humans ,Mesenteric arteries ,Observer Variation ,Reproducibility ,Ultrasonography, Doppler, Duplex ,Common hepatic artery ,business.industry ,Gastroenterology ,Reproducibility of Results ,Blood flow ,medicine.disease ,Surgery ,Mesenteric Arteries ,medicine.anatomical_structure ,Vascular resistance ,030211 gastroenterology & hepatology ,Female ,Vascular Resistance ,Radiology ,Clinical Competence ,business ,Observer variation ,Perfusion ,Blood Flow Velocity - Abstract
Introduction: The number of publications concerning mesenteric Doppler sonography (mesDS) is immense and does not correlate with the frequency of its use in clinical practice. This is astonishing since it provides real time blood flow (perfusion) information without side effects. Despite uncontrollable parameters like the technical limitations in some situations the optimization of (possibly) controllable parameters like standardization, production of normal values and reduction of the investigator variability by evaluating stable parameters could change the situation. Patients and methods: 10 investigators experienced in abdominal sonography (“DEGUM-Seminarleiter”) performed mesenteric Doppler sonography in 5 healthy subjects with 5 different machines. Results: The portal vein at the confluence and the common hepatic artery provide a significant portion of investigations with intromission angles of more than 60°. Values of diameter, resistance index and pulsatility index of the celiac trunc could be obtained with inter-observer variability values below 25 %. The proper and the common hepatic artery show no differences in inter-observer variability values, whereas the intrahepatic measure point of the portal vein showed a higher reproducibility. Discussion: We define frame conditions for future mesenteric Doppler studies: the portal vein should be investigated at the intrahepatic measure point. Pathophysiological studies should refrain from velocity parameters except in the case of larger vessels running in a straight course towards the probe.
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- 2016
17. The US7 score is sensitive to change in a large cohort of patients with rheumatoid arthritis over 12 months of therapy
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H. Sattler, Tina M Backhaus, J. Strunk, Sarah Ohrndorf, Wolfgang A. Schmidt, Christof Iking-Konert, Marina Backhaus, Wolfgang Hartung, Herbert Kellner, and Gerd R Burmester
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Adult ,Male ,medicine.medical_specialty ,Adolescent ,Hand Joints ,Immunology ,Rheumatoid Arthritis ,Sensitivity and Specificity ,General Biochemistry, Genetics and Molecular Biology ,Arthritis, Rheumatoid ,Cohort Studies ,Young Adult ,Rheumatology ,Foot Joints ,Synovitis ,Internal medicine ,medicine ,DAS28 ,Humans ,Immunology and Allergy ,Ultrasonography, Doppler, Color ,Young adult ,skin and connective tissue diseases ,Ultrasonography ,Aged ,Aged, 80 and over ,Tenosynovitis ,business.industry ,Clinical and Epidemiological Research ,Middle Aged ,medicine.disease ,Connective tissue disease ,Surgery ,Large cohort ,Treatment Outcome ,Antirheumatic Agents ,Rheumatoid arthritis ,Cohort ,Female ,business ,TNF-alpha ,Cohort study - Abstract
PurposeTo determine the sensitivity to change of the US7 score among RA patients under various therapies and to analyze the effect of each therapeutic option over 1 year. To estimate predictors for development of destructive bone changes.MethodsMusculoskeletal ultrasound (US7 score), DAS28, CRP and ESR were performed in 432 RA patients at baseline and after 3, 6 and 12 months. The cohort was divided into four sub-groups: first-line DMARDs (Group 1; 27.3%), therapy switch: DMARDs to second DMARDs (Group 2; 25.0%), first-line biologic after DMARDs therapy (Group 3; 35.4%) and therapy change from biologic to second biologic (Group 4; 12.3%).ResultsThe US7 synovitis and tenosynovitis sum scores in grey-scale (GSUS) and power Doppler ultrasound (PDUS) as well as ESR, CRP decreased significantly (pConclusionsThe comparable developments of the US7 score with clinical and laboratory data illustrates its potential to reflect therapeutic response. Therefore, the novel US7 score is sensitive to change. Patients who switched from one biologic to another exhibited a significant decline in erosions after 12 months, while the erosions scores in the other groups were stable.
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- 2012
18. Improved chemoenzymatic asymmetric synthesis of (S)-Rivastigmine
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Johann H. Sattler, Mathias Pickl, Wilfried Banko, Wolfgang Kroutil, Dominik Koszelewski, Katharina Tauber, Kurt Faber, Michael Fuchs, and Anja K. Holzer
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chemistry.chemical_classification ,Carbamate ,biology ,Transamination ,Stereochemistry ,medicine.medical_treatment ,Organic Chemistry ,Enantioselective synthesis ,biology.organism_classification ,Biochemistry ,chemistry ,Biocatalysis ,Drug Discovery ,medicine ,Side chain ,Amine gas treating ,Paracoccus denitrificans ,Pharmacophore - Abstract
(S)-Rivastigmine [(S)-1] was obtained via a four-step synthesis using an asymmetric enzymatic transamination protocol as the key step. An early introduction of the carbamate pharmacophore side chain avoided the use of protective group strategies and hence led to a considerable shortcut. This strategy required a novel ω-transaminase from Paracoccus denitrificans, which could transform the highly polar key substrate 3-acetylphenyl ethyl(methyl)carbamate (4) to the corresponding amine (S)-5 in 99% ee and >80% conversion.
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- 2012
19. Oxidation of Alcohols
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Francesco G. Mutti, Bo Yuan, Michael J. Karmilowicz, Nicholas J. Turner, Franck Escalettes, Johann H. Sattler, Wolfgang Kroutil, Carlos A. Martinez, Rajesh Kumar, Katharina Tauber, Nathan Wymer, and Jim Cawley
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Chemistry ,Alcohol oxidation ,Organic chemistry - Published
- 2012
20. Development and evaluation of a novel ultrasound score for large joints in rheumatoid arthritis: One year of experience in daily clinical practice
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Marina Backhaus, J. Strunk, H. Sattler, Boris Ehrenstein, Wolfgang A. Schmidt, Martin Fleck, Herbert Kellner, and Wolfgang Hartung
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musculoskeletal diseases ,medicine.medical_specialty ,medicine.diagnostic_test ,business.industry ,Arthritis ,medicine.disease ,Rheumatology ,Surgery ,Synovitis ,Erythrocyte sedimentation rate ,Rheumatoid arthritis ,Internal medicine ,Severity of illness ,Cohort ,medicine ,business ,Cohort study - Abstract
Objective To introduce and evaluate a new standardized ultrasound (US) score developed for large joints in patients with rheumatoid arthritis (RA). Methods A US score was designed to determine the degree of inflammation in the shoulder, the elbow, the hip, and the knee joint in patients with RA (Sonography of Large Joints in Rheumatology [SOLAR] score). Synovitis and synovial vascularity were scored semiquantitatively (grade 0–3) by gray-scale US (GSUS) and power Doppler US (PDUS). Patients with RA were examined at baseline and 3, 6, and 12 months after initiation of local or systemic therapy (disease-modifying antirheumatic drugs [DMARDs]/biologic agents). Erythrocyte sedimentation rate, anti–cyclic citrullinated peptide antibodies, and the clinical Disease Activity Score in 28 joints (DAS28) were determined. Results A cohort of 199 patients were analyzed and followed up over 12 months. At baseline, before modification of the therapy, patients received either DMARDs (n = 131), DMARDs plus biologic agents (n = 46), biologic monotherapy (n = 8), or no DMARD therapy (n = 14). At baseline, the mean DAS28 score was 4.6 and decreased to 3.2 after 1 year of therapy (P < 0.001). All US scores demonstrated a statistically significant improvement except for the PDUS scores for the shoulder and the hip. In detail, the mean synovitis GSUS score for the knee decreased from 5.2 at baseline to 2.2 after 12 months of followup. The mean GSUS score for the shoulder fell from 2.6 to 1.6, for the elbow fell from 5.2 to 2.6, and for the hip fell from 2.2 to 0.4 (P < 0.05 for each). Conclusion The SOLAR score is a feasible tool for the qualitative and quantitative evaluation of large joint involvement in patients with RA using US.
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- 2012
21. Möglichkeiten der Arthrosonografie in der Differenzialdiagnose bei entzündlich-rheumatischen Erkrankungen
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H. Sattler and T. N. Sattler
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Gynecology ,medicine.medical_specialty ,Rheumatology ,business.industry ,medicine ,business - Abstract
Die Sonografie ermoglicht sehr gut, allgemeine Arthritiszeichen zu erfassen. Sie hat aber ihre Grenze in der Differenzialdiagnose. Der folgende Beitrag versucht, die Moglichenkeiten und Grenzen der Sonografie bei entzundlich-rheumatischen Erkrankungen aufzuzeigen. Die endgultige Diagnose ist immer im Kontext von Laborergebnissen, klinischer Untersuchung und Bildgebung zu stellen.
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- 2012
22. Biocatalytic Organic Synthesis of Optically Pure (S)-Scoulerine and Berbine and Benzylisoquinoline Alkaloids
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Wolf-Dieter Lienhart, Silvia Wallner, Verena Resch, Wolfgang Kroutil, Johann H. Sattler, Peter Macheroux, Joerg H. Schrittwieser, and Jasmin Resch
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Stereochemistry ,Berberine Alkaloids ,010402 general chemistry ,Benzylisoquinolines ,01 natural sciences ,Chemical synthesis ,Article ,chemistry.chemical_compound ,Alkaloids ,Scoulerine ,Organic chemistry ,Bischler–Napieralski reaction ,Enantiomeric excess ,Molecular Structure ,010405 organic chemistry ,Chemistry ,Organic Chemistry ,Enantioselective synthesis ,Total synthesis ,Stereoisomerism ,Organic Chemistry Phenomena ,0104 chemical sciences ,Cyclization ,Biocatalysis ,Organic synthesis ,Oxidation-Reduction - Abstract
A chemoenzymatic approach for the asymmetric total synthesis of the title compounds is described that employs an enantioselective oxidative C-C bond formation catalyzed by berberine bridge enzyme (BBE) in the asymmetric key step. This unique reaction yielded enantiomerically pure (R)-benzylisoquinoline derivatives and (S)-berbines such as the natural product (S)-scoulerine, a sedative and muscle relaxing agent. The racemic substrates rac-1 required for the biotransformation were prepared in 4-8 linear steps using either a Bischler-Napieralski cyclization or a C1-Cα alkylation approach. The chemoenzymatic synthesis was applied to the preparation of fourteen enantiomerically pure alkaloids, including the natural products (S)-scoulerine and (R)-reticuline, and gave overall yields of up to 20% over 5-9 linear steps.
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- 2011
23. Biokatalytische enantioselektive oxidative C-C-Kupplung durch C-H-Aktivierung mit molekularem Sauerstoff
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Verena Resch, Peter Macheroux, Johann H. Sattler, Katharina Durchschein, Joerg H. Schrittwieser, Karl Gruber, Wolf-Dieter Lienhart, Andreas Winkler, and Wolfgang Kroutil
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Chemistry ,General Medicine - Published
- 2011
24. Recent biocatalytic oxidation–reduction cascades
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Verena Resch, Francesco G. Mutti, Johann H. Sattler, Wolfgang Kroutil, and Joerg H. Schrittwieser
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Bacteria ,010405 organic chemistry ,Chemistry ,Fungi ,Oxidation reduction ,010402 general chemistry ,01 natural sciences ,Biochemistry ,Combinatorial chemistry ,Article ,0104 chemical sciences ,Analytical Chemistry ,Enzymes ,Redox enzymes ,Biocatalysis ,Organic chemistry ,Animals ,Humans ,Oxidation-Reduction - Abstract
The combination of an oxidation and a reduction in a cascade allows performing transformations in a very economic and efficient fashion. The challenge is how to combine an oxidation with a reduction in one pot, either by running the two reactions simultaneously or in a stepwise fashion without isolation of intermediates. The broader availability of various redox enzymes nowadays has triggered the recent investigation of various oxidation–reduction cascades.
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- 2011
25. Stereoselectivity of Four (R)-Selective Transaminases for the Asymmetric Amination of Ketones
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Christine S. Fuchs, Johann H. Sattler, Francesco G. Mutti, Wolfgang Kroutil, and Desiree Pressnitz
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biology ,010405 organic chemistry ,Chemistry ,Stereochemistry ,Arthrobacter sp ,Substrate (chemistry) ,General Chemistry ,010402 general chemistry ,biology.organism_classification ,01 natural sciences ,0104 chemical sciences ,Transaminase ,Biocatalysis ,Amine gas treating ,Stereoselectivity ,Aspergillus terreus ,Amination - Abstract
Four (R)-ω-transaminases originating from Hyphomonas neptunium (HN-ωTA), Aspergillus terreus (AT-ωTA) and Arthrobacter sp. (ArR-ωTA), as well as an evolved transaminase (ArRmut11-ωTA) were successfully employed for the amination of prochiral ketones leading to optically pure (R)-amines. The first three transaminases displayed perfect stereoselectivity for the amination of all substrates tested (ee >99%). Furthermore, the transaminase AT-ωTA led in most cases to better conversion than ArR-ωTA and HN-ωTA using D-alanine as amine donor. α-Tetralone, which was the only substrate not accepted by HN-ωTA, ArR-ωTA, and AT-ωTA, was successfully transformed with perfect enantioselectivity (ee >99%) into the corresponding optically pure amine employing the variant ArRmut11-ωTA.
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- 2011
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26. Evaluation of a novel 7-joint ultrasound score in daily rheumatologic practice: A pilot project
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H. Sattler, G.-R. Burmester, H. Sörensen, Herbert Kellner, Wolfgang Hartung, J. Strunk, T. M. Backhaus, J. Kaufmann, Wolfgang A. Schmidt, Marina Backhaus, Katinka Albrecht, Karsten Becker, L. Meier, and Sarah Ohrndorf
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Adult ,Male ,musculoskeletal diseases ,medicine.medical_specialty ,Hand Joints ,Immunology ,Metatarsophalangeal joints ,Arthritis ,Pilot Projects ,Severity of Illness Index ,Arthritis, Rheumatoid ,Psoriatic arthritis ,Rheumatology ,Foot Joints ,Germany ,Synovitis ,Internal medicine ,medicine ,Humans ,Immunology and Allergy ,Rheumatoid factor ,Pharmacology (medical) ,Prospective Studies ,Aged ,Ultrasonography ,Aged, 80 and over ,Tenosynovitis ,Tumor Necrosis Factor-alpha ,business.industry ,Arthritis, Psoriatic ,Reproducibility of Results ,Middle Aged ,medicine.disease ,Surgery ,Radiography ,Treatment Outcome ,medicine.anatomical_structure ,Antirheumatic Agents ,Rheumatoid arthritis ,Female ,business - Abstract
Objective To introduce a new standardized ultrasound score based on 7 joints of the clinically dominant hand and foot (German US7 score) implemented in daily rheumatologic practice. Methods The ultrasound score included the following joints of the clinically dominant hand and foot: wrist, second and third metacarpophalangeal and proximal interphalangeal, and second and fifth metatarsophalangeal joints. Synovitis and synovial/tenosynovial vascularity were scored semiquantitatively (grade 0–3) by gray-scale (GS) and power Doppler (PD) ultrasound. Tenosynovitis and erosions were scored for presence. The scoring range was 0–27 for GS synovitis, 0–39 for PD synovitis, 0–7 for GS tenosynovitis, 0–21 for PD tenosynovitis, and 0–14 for erosions. Patients with arthritis were examined at baseline and after the start or change of disease-modifying antirheumatic drug (DMARD) and/or tumor necrosis factor α (TNFα) inhibitor therapy 3 and 6 months later. C-reactive protein level, erythrocyte sedimentation rate, rheumatoid factor, anti–cyclic citrullinated peptide, Disease Activity Score in 28 joints (DAS28), and radiographs of the hands and feet were performed. Results One hundred twenty patients (76% women) with rheumatoid arthritis (91%) and psoriatic arthritis (9%) were enrolled. In 52 cases (43%), erosions were seen in radiography at baseline. Patients received DMARDs (41%), DMARDs plus TNFα inhibitors (41%), or TNFα inhibitor monotherapy (18%). At baseline, the mean DAS28 was 5.0 and the synovitis scores were 8.1 in GS ultrasound and 3.3 in PD ultrasound. After 6 months of therapy, the DAS28 significantly decreased to 3.6 (Δ = 1.4), and the GS and PD ultrasound scores significantly decreased to 5.5 (−32%) and 2.0 (−39%), respectively. Conclusion The German US7 score is a viable tool for examining patients with arthritis in daily rheumatologic practice because it significantly reflects therapeutic response.
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- 2009
27. Der Frankfurt-Score: Eine arthrosonografische Stadieneinteilung bei rheumatischem Befall der Hand- und Fingergelenke
- Author
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H. Sattler, T. Schick, H. Eggers, and S. Rehart
- Subjects
musculoskeletal diseases ,medicine.medical_specialty ,medicine.diagnostic_test ,business.industry ,Cartilage ,Soft tissue ,Physical examination ,medicine.disease ,Surgery ,medicine.anatomical_structure ,Rheumatology ,Rheumatoid arthritis ,Clinical investigation ,Significant positive correlation ,Medicine ,Radiology ,Stage (cooking) ,skin and connective tissue diseases ,business ,Rheumatoide arthritis - Abstract
BACKGROUND: The early manifestation of the rheumatoid arthritis consists of a soft tissue affection of the joints. GOAL: Purpose of the study was the production of an arthrosonographic staging for the description of the soft tissue and cartilage/bone changes with rheumatoid arthritis of the hand and finger joints. Correlations of the arthrosonographic examination to the established DAS 28 as well as to parameters of the clinical examination were undertaken. PATIENTS AND METHODS: Clinical and arthrosonographic examinations as well as raising the established DAS 28 of 35 patients (with confirmed rheumatoid arthritis) and 10 control persons were carried out. The results werecompared and correlated to each other. RESULTS: A detailed staging can be made which exhibits a very high positive correlation to DAS 28 as well as to parameters of the clinical investigation. More than a quarter of the arthrosonographically determined joint changes correspond to the stage 1 (purely soft tissue changes) and would thus not be detectable radiologically. CONCLUSIONS: On account of the outstanding results of the arthrosonographic examination it is meaningful to establish anduse this imaging method as a standardized staging in the routine diagnostics of the rheumatoid arthritis.
- Published
- 2007
28. Sonografie der Hand- und Fingergelenke beim 'Rheumatiker' - Korrelation zum radiologischen Befund
- Author
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H. Sattler, T. Schick, S. Rehart, and H. Eggers
- Subjects
musculoskeletal diseases ,medicine.medical_specialty ,business.industry ,Early rheumatoid arthritis ,medicine.disease ,Surgery ,Disease activity ,Hand joint ,Rheumatology ,Rheumatoid arthritis ,medicine ,Ultrasonography ,skin and connective tissue diseases ,Nuclear medicine ,business ,Pathological ,Rheumatoide arthritis - Abstract
BACKGROUND: Manisfestations of rheumatoid arthritis in the musculoskeletal system affect above all the small joints of the hand and here initially the soft-tissue parts. Destruction of bone is assessed by means of established radiological classifications (e. g., “Larsen stages”). AIM: In this study a new sonographic system to assess the status of the hand and finger joints in a collective of rheumatoid arthritis patients is presented, validated and compared with conventional X-ray diagnostics. MATERIALS AND METHODS: The hand and finger joints of 20 patients with a confirmed diagnosis of rheumatoid arthritis were examined by means of ultrasonography and conventional X-ray methods. The results were compared and correlated. The DAS 28 score was used as the criterion to assess disease activity. RESULTS: In total, on sonography 20 % more joints showed pathological sot-tissue affections (synovialitis/effusions) than on X-ray diagnosis. The correlation of the sonographic assessment system with the DAS 28 score was higher than that with the X-ray findings. In the initial studies, even with low DAS 28 scores, joint affections, e. g., also erosions, could be detected. CONCLUSIONS: The high sensitivity (in totals, 20 % more joint affections were detected) and specificity (Spearman’s correlation coefficient between sonographic score and DAS 28 score = + 0.4) of the sonographic method justify its routine use in diagnosis and follow-up, especially in early rheumatoid arthritis as well. This method makes possible the early and secure detection of joint changes due to inflammation.
- Published
- 2007
29. Kontrastmitelsonografie bei Coxitis, nützlich oder nur teuer?
- Author
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C Löffler and H Sattler
- Subjects
Radiology, Nuclear Medicine and imaging - Published
- 2015
30. Standardebenen und dynamische sonographische Untersuchung an den Bewegungsorganen aus rheumatologischer Sicht
- Author
-
H. Sattler
- Subjects
Rheumatology ,business.industry ,Medicine ,business - Published
- 2006
31. Bildgebende Verfahren in der Rheumatologie: Sonographie bei rheumatoider Arthritis
- Author
-
H. Sattler, Marina Backhaus, Herbert Kellner, and Wolfgang A. Schmidt
- Subjects
Gynecology ,medicine.medical_specialty ,Rheumatology ,business.industry ,Ultrasound imaging ,Medicine ,Ultrasonography ,business ,Rheumatoide arthritis - Abstract
Die Sonographie der Bewegungsorgane ist ein wichtiges bildgebendes Verfahren in der Diagnostik der rheumatoiden Arthritis und Bestandteil der Weiterbildung zum Rheumatologen in Deutschland. Daruber hinaus werden zahlreiche Kurse angeboten, um spezielle Kenntnisse und praktische Fahigkeiten zu erwerben. Die internationale Bedeutung der Sonographie in der Rheumatologie hat in den letzten funf Jahren erheblich zugenommen. Die Sonographie kann in der Praxis und am Krankenbett als Fortsetzung des klinischen Status durchgefuhrt werden. Sie ist fur den Patienten nicht belastend und kann jederzeit und beliebig oft wiederholt werden. Der Einfluss auf therapeutische Entscheidungen ist erheblich. Voraussetzung ist ein Ultraschallgerat mit einem Linearschallkopf mit ≥5 MHz. Am haufigsten werden Schallkopfe mit ca.7,5 MHz verwendet. Moderne, hoherfrequente, hochauflosende Schallkopfe (>7,5 MHz) erlauben zusatzliche diagnostische Aussagen. Die Sonographie ist dem konventionellen Rontgen beim Nachweis von Erosionen uberlegen soweit die knochernen Lasionen der sonographischen Untersuchung zuganglich sind. Sie ist sensitiver als die klinische Untersuchung, was den Nachweis von Synovitiden, Tenosynovitiden, Tendinitiden und Bursitiden sowie deren Differenzierung voneinander betrifft. Die Farbdoppler-Sonographie ermoglicht daruber hinaus die Einschatzung der Entzundungsaktivitat und hilft bei der Differenzierung intraartikularer Strukturen. Carpal- und Ulnartunnelsyndrom konnen bei der rheumatoiden Arthritis auftreten. Sie sind haufig mit einer sonographisch nachweisbaren charakteristischen Schwellung der Nerven verbunden. Punktionen und Injektionen konnen sonographie-gestutzt oder sonographie-gesteuert durchgefuhrt werden.
- Published
- 2003
32. Technik und Stellenwert der Arthrosonographie in der rheumatologischen Diagnostik
- Author
-
H. Sattler, Wolfgang A. Schmidt, M. Bohl-Bühler, Jürgen Braun, R.-W. Hauer, Marina Backhaus, Holger Mellerowicz, and Dietrich Banzer
- Subjects
Value (ethics) ,medicine.medical_specialty ,Supraspinatus muscle ,Standardization ,media_common.quotation_subject ,Sternoclavicular joint ,Osteoarthritis ,Knee Joint ,Rheumatology ,Synovitis ,medicine ,Acromioclavicular joint ,Quality (business) ,Medical physics ,Rotator cuff ,Acromion ,media_common ,Gynecology ,business.industry ,Ultrasound ,Frozen shoulder ,medicine.disease ,medicine.anatomical_structure ,Ultrasound imaging ,Shoulder joint ,Ultrasonography ,business ,Nuclear medicine ,Quality assurance - Abstract
Der Einsatz der Arthrosonographie an der Hand gewinnt insbesondere in der Fruharthritisdiagnostik eine zunehmende Bedeutung. Sehr sensitiv gelingt der Nachweis kleinster Flussigkeitsansammlungen in den Gelenken und Sehnenscheiden sowie das Aufdecken kleinster erosiver Knochenlasionen mitunter eher als mit der konventionellen Rontgendiagnostik. Die Arthrosonographie ist ebenfalls hilfreich bei der morphologischen Analyse der Veranderungen des Nervus medianus beim Karpaltunnel-Syndrom. Folgende Standardschnittebenen werden fur die sonographische Untersuchung am Handgelenk empfohlen: 1. dorsaler Longitudinalschnittuber dem Radiokarpal- und 2. Ulnokarpalgelenk mit der Frage nach Gelenkerguss und Synovialisproliferation, Ganglien, Sehnenscheidenentzundungen, Unregelmasigkeiten der knochernen Oberflache wie bei knochernen Appositionen sowie bei Erosionen und 3. dorsaler Transversalschnitt uber dem Handgelenk zur Darstellung der Strukturen in einer weiteren Ebene, 4. volarer Longitudinalschnitt uber dem Radiocarpal- und 5. Ulnokarpalgelenk und 6. volarer Transversalschnittuber dem Handgelenk mit gleicher Fragestellung wie in den vorgenannten Ebenen sowie zur Beurteilung des Nervus medianus im Rahmen der Abklarung eines Karpaltunnel-Syndroms, optional 7. ulnarer Longitudinal- und 8. Transversalschnitt uber dem ulnaren Gelenkspalt und dem M. extensor carpi ulnaris zur Erfassung einer Tenosynovitis in diesem Bereich sowie des Caput ulnae Syndroms, 9. radialer Longitudinal- und 10. Transversalschnitt mit gleicher Fragestellung wie in den vorgenannten Ebenen. An den Fingergelenken werden folgende Standardschnittebenen fur die sonographische Untersuchung empfohlen: 1.volarer Longitudinal- und 2.volarer Transversalschnitt in Extension uber dem Daumensattelgelenk (DSG), MCP-, PIP- und DIP-Gelenken mit der Frage nach Gelenkerguss und Synovialisproliferation, Sehnenscheidenentzundungen, Unregelmasigkeiten der knochernen Oberflache wie bei knochernen Appositionen (Osteophyten) sowie bei Erosionen, 3. dorsaler Longitudinal und 4.dorsaler Transversalschnittin Extension und Flexion von ca. 70° uber den MCP-, PIP- und DIP-Gelenken sowie dem DSG in Extension mit der Frage nach Gelenkerguss und Synovialisproliferation, Sehnenscheidenentzundungen bzw. Tendinitis, Unregelmasigkeiten der knochernen Oberflache wie bei Arthrose sowie bei Erosionen, optional 5. Medialer Longitudinalschnitt in Hohe der MCP-Gelenke I, II, sowie der PIP- und DIP-Gelenke und 6. lateraler Longitudinalschnitt in Hohe des MCP-Gelenkes V sowie der PIP- und DIP-Gelenke zur Erfassung von Erosionen und Instabilitaten. Verwendet werden sollten Linearschallsonden mit einer Frequenz von etwa 7,5–12 MHz. Ein Erguss oder eine Synovialitis im Handgelenk gelten als wahrscheinlich bei einem ventralen Knochen-Gelenkkapsel-Abstand von ≥3 mm und als sicher bei ≥4 mm. Er ist wahrscheinlich bei einer Seitendifferenz von ≥1mm und sicher bei ≥2 mm. Bei einer Querschnittsflache des Nervus medianus von ≥12 mm2 ist ein Karpaltunnel-Syndrom wahrscheinlich.
- Published
- 2002
33. Technik und Stellenwert der Arthrosonographie in der rheumatologischen Diagnostik Teil 2: Sonographie der Hüftregion
- Author
-
M. Bohl-Bühler, Marina Backhaus, Jürgen Braun, H. Mellerowicz, H. Sattler, Wolfgang A. Schmidt, R.-W. Hauer, and D. Banzer
- Subjects
Gynecology ,medicine.medical_specialty ,Rheumatology ,business.industry ,Ultrasound imaging ,medicine ,Ultrasonography ,business - Abstract
Die Arthrosonographie im Bereich der Huftregion hat eine grose Bedeutung als leicht einzusetzende, kostengunstige und patientenfreundliche Untersuchungsmethode, da die klinische Untersuchung bei rheumatischen Fragestellungen oft unzureichend ist. Folgende Standardebenen werden empfohlen: 1. ventraler Longitudinalschnitt mit der Frage nach Gelenkerguss, Bursitis iliopectinea, Unregelmasigkeiten der knochernen Oberflache bei Arthrose, Morbus Perthes sowie bei Erosionen, 2. ventraler Transversalschnitt zur Darstellung der Strukturen in einer weiteren Ebene, 3. lateraler Longitudinalschnitt am Huftgelenk mit gleicher Fragestellung wie in den vorgenannten Ebenen sowie 4. lateraler Longitudinalschnitt und 5. lateraler Transversalschnitt am Trochanter major zur Beurteilung einer Bursitis trochanterica und knochernen Unregelmasigkeiten bei Enthesiopathien und 6. optional ein dorsaler Schragschnitt. Dabei konnen seltenere, ausschlieslich dorsal lokalisierte Huftgelenkergusse und Pannus nachgewiesen werden. Eine rotierende Gelenkbewegung ist unerlasslich, um kleine Ergusse zu erfassen. Verwendet werden sollten Schallkopfe mit 5 bis 7,5MHz. Bei adiposen oder muskularen Patienten muss gelegentlich wegen der notwendigen Eindringtiefe auf einen 3,5MHz Schallkopf ausgewichen werden. Ein Huftgelenkerguss oder eine Synovialitis gelten als wahrscheinlich bei einem ventralen Knochen-Gelenkkapsel-Abstand von ≥7mm und als sicher bei ≥8mm. Er ist wahrscheinlich bei einer Seitendifferenz von ≥2mm und sicher bei ≥3mm.
- Published
- 2002
34. Stereoselective synthesis of γ-hydroxynorvaline through combination of organo- and biocatalysis
- Author
-
Joerg H. Schrittwieser, Wolfgang Kroutil, Johann H. Sattler, Jörg Pietruszka, Robert C. Simon, Eduardo Busto, and Kurt Faber
- Subjects
Ketone ,Reducing agent ,Catalysis ,Stereocenter ,2-Propanol ,Materials Chemistry ,Organic chemistry ,Mannich reaction ,chemistry.chemical_classification ,Binding Sites ,Metals and Alloys ,Diastereomer ,Stereoisomerism ,Valine ,General Chemistry ,Ketones ,Butyrophenones ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,Protein Structure, Tertiary ,Molecular Docking Simulation ,Alcohol Oxidoreductases ,chemistry ,Biocatalysis ,Reducing Agents ,Yield (chemistry) ,ddc:540 ,Ceramics and Composites ,Solvents ,Stereoselectivity ,Oxidation-Reduction ,NADP - Abstract
An efficient route for the synthesis of all four diastereomers of PMP-protected α-amino-γ-butyrolacton to access γ-hydroxynorvaline was established. The asymmetric key steps comprise an organocatalytic Mannich reaction and an enzymatic ketone reduction. Three reaction steps could be integrated in a one-pot process, using 2-PrOH both as solvent and as reducing agent. The sequential construction of stereogenic centres gave access to each of the four stereoisomers in high yield and with excellent stereocontrol.
- Published
- 2014
35. Sonografische Detektion intraossärer Läsionen im Vergleich zwischen gesundem und osteoporotischem Knochen – eine in-vitro Studie
- Author
-
A Ficklscherer, P Müller, M Pietschmann, TN Sattler, and H Sattler
- Subjects
Radiology, Nuclear Medicine and imaging - Published
- 2014
36. Steric vs. electronic effects in the Lactobacillus brevis ADH-catalyzed bioreduction of ketones
- Author
-
Johann H. Sattler, Iván Lavandera, Cristina Rodríguez, Wolfgang Kroutil, Wioleta Borzęcka, and Vicente Gotor
- Subjects
chemistry.chemical_classification ,Steric effects ,Models, Molecular ,Ketone ,Molecular Structure ,Aryl ,Organic Chemistry ,Levilactobacillus brevis ,Substituent ,Alcohol Dehydrogenase ,Electrons ,Ketones ,Biochemistry ,chemistry.chemical_compound ,chemistry ,Propiophenone ,Alcohols ,Electronic effect ,Organic chemistry ,Physical and Theoretical Chemistry ,Oxidation-Reduction ,Alkyl ,Acetophenone - Abstract
Lactobacillus brevis ADH (LBADH) is an alcohol dehydrogenase that is commonly employed to reduce alkyl or aryl ketones usually bearing a methyl, an ethyl or a chloromethyl as a small ketone substituent to the corresponding (R)-alcohols. Herein we have tested a series of 24 acetophenone derivatives differing in their size and electronic properties for their reduction employing LBADH. After plotting the relative activity against the measured substrate volumes we observed that apart from the substrate size other effects must be responsible for the activity obtained. Compared to acetophenone (100% relative activity), other small substrates such as propiophenone, α,α,α-trifluoroacetophenone, α-hydroxyacetophenone, and benzoylacetonitrile had relative activities lower than 30%, while medium-sized ketones such as α-bromo-, α,α-dichloro-, and α,α-dibromoacetophenone presented relative activities between 70% and 550%. Moreover, the comparison between the enzymatic activity and the obtained final conversions using an excess or just 2.5 equiv. of the hydrogen donor 2-propanol, denoted again deviations between them. These data supported that these hydrogen transfer (HT) transformations are mainly thermodynamically controlled. For instance, bulky α-halogenated derivatives could be quantitatively reduced by LBADH even employing 2.5 equiv. of 2-propanol independently of their kinetic values. Finally, we found good correlations between the IR absorption band of the carbonyl groups and the degrees of conversion obtained in these HT processes, making this simple method a convenient tool to predict the success of these transformations.
- Published
- 2014
37. Creating a Biocatalyst for the Production of an Optically Pure Sterically Hindered Amine
- Author
-
Katharina Tauber, Johann H. Sattler, Francesco G. Mutti, and Wolfgang Kroutil
- Subjects
Steric effects ,010405 organic chemistry ,Chemistry ,Organic Chemistry ,Enantioselective synthesis ,010402 general chemistry ,01 natural sciences ,Catalysis ,0104 chemical sciences ,Enzyme catalysis ,Inorganic Chemistry ,Biocatalysis ,Organic chemistry ,Amine gas treating ,Physical and Theoretical Chemistry ,Amination - Published
- 2010
38. Evaluation of the novel ultrasound score for large joints in psoriatic arthritis and ankylosing spondylitis: six month experience in daily clinical practice
- Author
-
H. Sattler, Martin Fleck, Boris Ehrenstein, Wolfgang A. Schmidt, Marina Backhaus, Valentin S. Schäfer, Herbert Kellner, Wolfgang Hartung, and J. Strunk
- Subjects
Adult ,Male ,musculoskeletal diseases ,medicine.medical_specialty ,Sports medicine ,medicine.medical_treatment ,Severity of Illness Index ,Psoriatic arthritis ,Rheumatology ,Internal medicine ,Large joints ,Ultrasound ,Epidemiology ,medicine ,Humans ,Spondylitis, Ankylosing ,Orthopedics and Sports Medicine ,Ultrasonography ,Ankylosing spondylitis ,Rehabilitation ,business.industry ,Arthritis, Psoriatic ,Middle Aged ,medicine.disease ,Antirheumatic Agents ,Orthopedic surgery ,Cohort ,Physical therapy ,Female ,Joints ,business ,Scoring ,Research Article - Abstract
Background To evaluate the utility of the recently introduced SOLAR score (sonography of large joints in Rheumatology), which has been validated in RA patients, in a cohort of patients with Psoriatic Arthritis (PsA) and Ankylosing Spondylitis (AS) presenting with involvement of large peripheral joints. Methods The recently established SOLAR score has been designed to determine the degree of inflammation in the shoulder, the elbow, the hip and the knee joint in patients suffering from RA. Since large joints are frequently involved in PsA and AS, synovitis and synovial vascularity were scored semiquantitatively (grade 0–3) by grey scale (GSUS) and power Doppler ultrasound (PDUS) utilizing the validated scoring system. Each joint was scanned from different angles, the knee joint for example was divided into four areas to score for synovitis: the suprapatellar longitudinal, the medial longitudinal, the lateral longitudinal, and the posterior region. Each area was scored from 0–3, so a maximum score of 12 could be achieved. PsA and AS patients presenting with peripheral joint disease involving large joints were examined at baseline, 3 and 6 months after initiation of local or systemic therapy (DMARDs/Biologics). For evaluation of the inflammatory status, the erythrocyte sedimentation rate (ESR) was determined. Results A cohort of 126 patients were enclosed, and 83 of these were followed for 6 months. At baseline before modification of the therapy, patients received DMARDs (n = 83), DMARDs plus biologics (n = 30), or biologic monotherapy (n = 29). Following intervention, all US scores demonstrated a marked improvement. The GSUS and the PDUS scores for all joint areas, except the PDUS score of the hip, exhibited a significant improvement (p Conclusion The SOLAR score, which has been recently introduced for RA patients, is a very suitable instrument for the qualitative and quantitative evaluation of large joint involvement in PsA and AS patients and allows for treatment monitoring.
- Published
- 2013
39. Asymmetric Preparation of
- Author
-
Wolfgang, Kroutil, Eva-Maria, Fischereder, Christine S, Fuchs, Horst, Lechner, Francesco G, Mutti, Desiree, Pressnitz, Aashrita, Rajagopalan, Johann H, Sattler, Robert C, Simon, and Elina, Siirola
- Subjects
Review - Abstract
This account focuses on the application of ω-transaminases, lyases, and oxidases for the preparation of amines considering mainly work from our own lab. Examples are given to access α-chiral primary amines from the corresponding ketones as well as terminal amines from primary alcohols via a two-step biocascade. 2,6-Disubstituted piperidines, as examples for secondary amines, are prepared by biocatalytical regioselective asymmetric monoamination of designated diketones followed by spontaneous ring closure and a subsequent diastereoselective reduction step. Optically pure tert-amines such as berbines and N-methyl benzylisoquinolines are obtained by kinetic resolution via an enantioselective aerobic oxidative C–C bond formation.
- Published
- 2013
40. OP0124 Imaging Remission by Musculoskeletal Ultrasound Leads To A Better Functional Outcome – Results of The US Impera Study - Us 7-Score Implementation Study in Early Rheumatoid Arthritis
- Author
-
G.-R. Burmester, Wolfgang A. Schmidt, Anne-Marie Glimm, Marina Backhaus, Sarah Ohrndorf, G. Schmittat, H. Sattler, Wolfgang Hartung, J. Strunk, Herbert Kellner, and I. Fischer
- Subjects
musculoskeletal diseases ,0301 basic medicine ,medicine.medical_specialty ,Visual analogue scale ,Immunology ,Subgroup analysis ,Musculoskeletal ultrasound ,Outcome (game theory) ,General Biochemistry, Genetics and Molecular Biology ,03 medical and health sciences ,0302 clinical medicine ,Rheumatology ,Internal medicine ,medicine ,Immunology and Allergy ,skin and connective tissue diseases ,030203 arthritis & rheumatology ,medicine.diagnostic_test ,business.industry ,Ultrasound ,Imaging Procedures ,Early rheumatoid arthritis ,030104 developmental biology ,Erythrocyte sedimentation rate ,Physical therapy ,business - Abstract
Background Novel treatment options together with a treat-to-target strategy present new challenges to imaging procedures in terms of therapy monitoring. Objectives To evaluate the functional outcome measured by Health Assessment Questionnaire (HAQ) as the primary outcome in patients with early rheumatoid arthritis (RA) who were treated by standard clinical care (clinical cohort) in comparison to patients in whom treatment decisions were additionally based on musculoskeletal ultrasound (US) using the US7 score (US-cohort) in a nationwide investigator initiated study in Germany. Methods Functional (HAQ), clinical (DAS28, patient9s Visual Analogue Scale [VAS] for disease activity) and laboratory parameters (erythrocyte sedimentation rate [ESR], C-reactive protein [CRP]) were analyzed at seven different time points for a total of 18 months and compared between the two study cohorts. Treatment decision was made according to local standard of care (SOC) with a treatment goal of DAS28 Results Data of 313 patients (US-cohort [n=166], clinical cohort [n=147]) demonstrated a reduction of HAQ, DAS28, VAS and laboratory activity (ESR, CRP) to a low disease activity status 18 months after the beginning/change of antirheumatic therapy according to SOC (table 1). The results of functional and clinical outcome in both cohorts did not show significant differences. However, subgroup analysis of the US-cohort with imaging remission resulted in a significantly lower HAQ, DAS28 and VAS compared to patients not fulfilling these US remission criteria (table 1). Conclusions Treatment strategies by a treat to target approach in early RA patients reduced disease activity and improved functional outcome of HAQ irrespective of imaging guidance. However, patients with imaging remission by US exhibited a significantly better functional outcome and lower disease activity status. Accordingly, an additional ultrasound examination aiming at imaging remission should be strongly considered in clinical practice. Disclosure of Interest None declared
- Published
- 2016
41. Zur Bedeutung des Doppelkonturzeichens (DKZ) bei Kristallarthropathie (Arthritis urica und Calcium-Pyro-Phosphat-Arthritis) – Eine Überprüfung der Arthrosonografie mit polarisationsoptischer Mikroskopie
- Author
-
V. Schmitt, L. Peters, H Sattler, and R Bergner
- Subjects
Radiology, Nuclear Medicine and imaging - Published
- 2012
42. Sonographie bei rheumatischer Omarthritis
- Author
-
H. Sattler
- Subjects
Gynecology ,medicine.medical_specialty ,business.industry ,medicine ,Orthopedics and Sports Medicine ,business - Abstract
Es gibt fruhe unspezifische und spate spezifische Zeichen einer Omarthritis. Je mehr spezifische Zeichen zusammengetragen werden konnen, desto sicherer ist die Diagnose, die Verlaufsbeobachtungen erfordert. Besonders wichtige Schnitte sind axillare Quer- und Langsschnitte, um die perikapitale Synovialitis und fruhe unspezifische Ergusse im axillaren Recessus zu sehen. Destruktionen wie Usuren sind hochspezifisch.
- Published
- 2002
43. Biocatalytic enantioselective oxidative C-C coupling by aerobic C-H activation
- Author
-
Verena Resch, Andreas Winkler, Peter Macheroux, Johann H. Sattler, Wolfgang Kroutil, Wolf-Dieter Lienhart, Joerg H. Schrittwieser, Karl Gruber, and Katharina Durchschein
- Subjects
Oxidative Coupling ,010405 organic chemistry ,Chemistry ,Stereochemistry ,Berberine Alkaloids ,Enantioselective synthesis ,Stereoisomerism ,Oxidoreductases, N-Demethylating ,General Chemistry ,Oxidative phosphorylation ,010402 general chemistry ,01 natural sciences ,Benzylisoquinolines ,Catalysis ,0104 chemical sciences ,Enzyme catalysis ,Oxygen ,Biocatalysis ,Organic chemistry ,Combinatorial Chemistry Techniques ,Oxidative coupling of methane - Published
- 2010
44. Interobserver-Varianzprüfung zur Darstellung der Vaskularisation bei Tenovaginitis der langen Bizepssehne
- Author
-
W Hartung and H Sattler
- Subjects
Radiology, Nuclear Medicine and imaging - Published
- 2010
45. Lässt sich die 'moribunde' Meniskussonografie durch Einsatz der Elastografie reanimieren?
- Author
-
H Sattler
- Subjects
Radiology, Nuclear Medicine and imaging - Published
- 2010
46. Dynamic MR an den Bewegungsorganen – eine neue Technik zur Bildverbesserung – tatsächlicher Fortschritt oder nur 'nice to have it'
- Author
-
H Sattler
- Subjects
Radiology, Nuclear Medicine and imaging - Published
- 2010
47. Kontrastmittelsonografie und Elastografie im Vergleich mit Powerdopplersonografie und Kontrastmittel-MRT am Beispiel der Tenosynovitis im Handgelenk- und Hohlhandbereich
- Author
-
A Zahn, S Briem, MA Weber, HM Lorenz, P Hallscheidt, and H Sattler
- Subjects
Radiology, Nuclear Medicine and imaging - Published
- 2010
48. Prof. Dr. C. H. Sattler University Eye Clinic, Breslau Head: Prof. Dr. A. Bielschowsky. Experiences with the elimination of amblyopia and the restoration of binocular vision in squinters, Part II. 1927
- Author
-
C H, Sattler
- Subjects
Strabismus ,Vision, Binocular ,Humans ,History, 20th Century ,Amblyopia - Published
- 2010
49. Ist die dorsale Hüftgelenkssonografie notwendig?
- Author
-
H Sattler
- Subjects
Radiology, Nuclear Medicine and imaging - Published
- 2009
50. Erste Erfahrungen im Einsatz des POWER MODE (CDI) zur Darstellung der Angioneogenese bei 'Achillodynie'
- Author
-
H Sattler
- Subjects
Radiology, Nuclear Medicine and imaging - Published
- 2009
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