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202 results on '"Hütter, Gero"'

1. Dynamics of virological and immunological markers of HIV persistence after allogeneic haematopoietic stem-cell transplantation in the IciStem cohort: a prospective observational cohort study

Author

Salgado, Maria, primary, Gálvez, Cristina, additional, Nijhuis, Monique, additional, Kwon, Mi, additional, Cardozo-Ojeda, E Fabian, additional, Badiola, Jon, additional, Gorman, Matthew J, additional, Huyveneers, Laura E P, additional, Urrea, Victor, additional, Bandera, Alessandra, additional, Jensen, Björn-Erik Ole, additional, Vandekerckhove, Linos, additional, Jurado, Manuel, additional, Raj, Kavita, additional, Schulze zur Wiesch, Julian, additional, Bailén, Rebeca, additional, Eberhard, Johanna M, additional, Nabergoj, Mitja, additional, Hütter, Gero, additional, Saldaña-Moreno, Raquel, additional, Oldford, Sharon, additional, Barrett, Lisa, additional, Ramirez, Maria Luisa Montes, additional, Garba, Salisu, additional, Gupta, Ravi Kumar, additional, Revollo, Boris, additional, Ferra-Coll, Christelle, additional, Kuball, Jurgen, additional, Alter, Galit, additional, Sáez-Cirión, Asier, additional, Diez-Martin, Jose Luis, additional, Duke, Elizabeth R, additional, Schiffer, Joshua T, additional, Wensing, Annemarie, additional, Martinez-Picado, Javier, additional, Muscatello, Antonio, additional, Calmy, Alexandra, additional, Chapel, Anais, additional, Mamez, Anne-Claire, additional, Passaes, Caroline, additional, Brisseau, Clarissa, additional, Olivarria, Eduardo, additional, Knops, Elena, additional, Heger, Eva, additional, Bikhezar, Fatima, additional, Perdomo-Celis, Federico, additional, Kobbe, Guido, additional, Gabriel, Ian H, additional, Lunzen, Jan V, additional, Dalmau, Judith, additional, Martín-Carbonero, Luz, additional, Puertas, Mari Carmen, additional, Garcia-Guerrero, Mari Carmen, additional, de Scheerder, Marie-Angélique, additional, Angin, Mathieu, additional, Lübke, Nadine, additional, Balsalobre, Pascual, additional, de Paz, Raquel, additional, Kaiser, Rolf, additional, Kerre, Tessa, additional, Harrer, Thomas, additional, Luedde, Tom, additional, and Rocha, Vanderson, additional

Published
2024
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2. Salvage treatment with plerixafor in poor mobilizing allogeneic stem cell donors: results of a prospective phase II-trial

Author

Hölig, Kristina, Schmidt, Helmuth, Hütter, Gero, Kramer, Michael, Teipel, Raphael, Heidrich, Katharina, Zimmer, Kristin, Heidenreich, Falk, Blechschmidt, Matthias, Torosian, Tigran, Ordemann, Rainer, Kroschinsky, Frank, Rücker-Braun, Elke, Gopsca, Laszlo, Wagner-Drouet, Eva Maria, Oelschlaegel, Uta, Schmidt, Alexander H., Bornhäuser, Martin, Ehninger, Gerhard, and Schetelig, Johannes

Published
2021
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3. Dependence on the CCR5 Coreceptor for Viral Replication Explains the Lack of Rebound of CXCR4-Predicted HIV Variants in the Berlin Patient

Author

Symons, Jori, Vandekerckhove, Linos, Hütter, Gero, Wensing, Annemarie MJ, van Ham, Petra M, Deeks, Steven G, and Nijhuis, Monique

Subjects
Medical Microbiology, Biomedical and Clinical Sciences, Immunology, Stem Cell Research, Transplantation, HIV/AIDS, Stem Cell Research - Nonembryonic - Human, Regenerative Medicine, Sexually Transmitted Infections, Infectious Diseases, Genetics, Infection, HIV Infections, HIV-1, Humans, Leukemia, Myeloid, Acute, Receptors, CCR5, Receptors, CXCR4, Receptors, HIV, Stem Cell Transplantation, Treatment Outcome, Viral Tropism, Virus Replication, HIV, cure, Berlin-Patient, coreceptor, stem cell transplantation, Biological Sciences, Medical and Health Sciences, Microbiology, Clinical sciences
Abstract

The "Berlin patient" is the first patient cured of HIV-1 infection after allogeneic transplantation with nonfunctional CCR5 coreceptor stem cells. We demonstrate that CXCR4-predicted minority viruses present prior to transplantation were unable to rebound after transplantation due to their dependence on CCR5 for replication and high genetic barrier toward CXCR4 usage.

Published
2014

4. HIV Antibody Characterization as a Method to Quantify Reservoir Size During Curative Interventions

Author

Burbelo, Peter D, Bayat, Ahmad, Rhodes, Craig S, Hoh, Rebecca, Martin, Jeffrey N, Fromentin, Rémi, Chomont, Nicolas, Hütter, Gero, Kovacs, Joseph A, and Deeks, Steven G

Subjects
Medical Microbiology, Biomedical and Clinical Sciences, Immunology, Infectious Diseases, HIV/AIDS, Biotechnology, Sexually Transmitted Infections, Stem Cell Research - Nonembryonic - Human, Stem Cell Research, Clinical Research, Infection, Good Health and Well Being, Adult, DNA, Viral, Gene Expression Regulation, Viral, HIV Antibodies, HIV Infections, Human Immunodeficiency Virus Proteins, Humans, Immunity, Humoral, Male, Stem Cell Transplantation, antibodies, elite controllers, HIV-1, HIV-1 persistence, serology, viral reservoirs, Biological Sciences, Medical and Health Sciences, Microbiology, Biological sciences, Biomedical and clinical sciences, Health sciences
Abstract

Quantitative humoral profiling of recent samples from a human immunodeficiency virus (HIV)-infected adult who was cured following a delta32/delta32 CCR5 stem cell transplant in 2007 revealed no antibodies against p24, matrix, nucleocapsid, integrase, protease, and gp120, but low levels of antibodies against reverse transcriptase, tat, and gp41. Antibody levels to these HIV proteins persisted at high and stable levels in most noncontrollers, elite controllers, and antiretroviral-treated subjects, but a rare subset of controllers had low levels of antibodies against matrix, reverse transcriptase, integrase, and/or protease. Comprehensive HIV antibody profiles may prove useful for monitoring curative interventions.

Published
2014

6. Differences in Cellular Composition of Peripheral Blood Stem Cell Grafts from Healthy Stem Cell Donors Mobilized with Either Granulocyte Colony-Stimulating Factor (G-CSF) Alone or G-CSF and Plerixafor

Author

Teipel, Raphael, Oelschlägel, Uta, Wetzko, Katrin, Schmiedgen, Maria, Kramer, Michael, Rücker-Braun, Elke, Hölig, Kristina, von Bonin, Malte, Heidrich, Katharina, Fuchs, Anke, Ordemann, Rainer, Kroschinsky, Frank, Bornhäuser, Martin, Hütter, Gero, Schmidt, Helmuth, Ehninger, Gerhard, Schetelig, Johannes, and Heidenreich, Falk

Published
2018
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7. Challenges in Detecting HIV Persistence during Potentially Curative Interventions: A Study of the Berlin Patient

Author

Yukl, Steven A, Boritz, Eli, Busch, Michael, Bentsen, Christopher, Chun, Tae-Wook, Douek, Daniel, Eisele, Evelyn, Haase, Ashley, Ho, Ya-Chi, Hütter, Gero, Justement, J Shawn, Keating, Sheila, Lee, Tzong-Hae, Li, Peilin, Murray, Danielle, Palmer, Sarah, Pilcher, Christopher, Pillai, Satish, Price, Richard W, Rothenberger, Meghan, Schacker, Timothy, Siliciano, Janet, Siliciano, Robert, Sinclair, Elizabeth, Strain, Matt, Wong, Joseph, Richman, Douglas, and Deeks, Steven G

Subjects
Medical Microbiology, Biomedical and Clinical Sciences, Immunology, Biotechnology, Clinical Research, Transplantation, HIV/AIDS, Sexually Transmitted Infections, Infectious Diseases, Genetics, 4.1 Discovery and preclinical testing of markers and technologies, Infection, Adult, Allografts, Anti-Retroviral Agents, Antibodies, Viral, DNA, Viral, HIV, HIV Infections, Hematopoietic Stem Cell Transplantation, Humans, RNA, Viral, Microbiology, Virology, Medical microbiology
Abstract

There is intense interest in developing curative interventions for HIV. How such a cure will be quantified and defined is not known. We applied a series of measurements of HIV persistence to the study of an HIV-infected adult who has exhibited evidence of cure after allogeneic hematopoietic stem cell transplant from a homozygous CCR5Δ32 donor. Samples from blood, spinal fluid, lymph node, and gut were analyzed in multiple laboratories using different approaches. No HIV DNA or RNA was detected in peripheral blood mononuclear cells (PBMC), spinal fluid, lymph node, or terminal ileum, and no replication-competent virus could be cultured from PBMCs. However, HIV RNA was detected in plasma (2 laboratories) and HIV DNA was detected in the rectum (1 laboratory) at levels considerably lower than those expected in ART-suppressed patients. It was not possible to obtain sequence data from plasma or gut, while an X4 sequence from PBMC did not match the pre-transplant sequence. HIV antibody levels were readily detectable but declined over time; T cell responses were largely absent. The occasional, low-level PCR signals raise the possibility that some HIV nucleic acid might persist, although they could also be false positives. Since HIV levels in well-treated individuals are near the limits of detection of current assays, more sensitive assays need to be developed and validated. The absence of recrudescent HIV replication and waning HIV-specific immune responses five years after withdrawal of treatment provide proof of a clinical cure.

Published
2013

9. Correction to: Salvage treatment with plerixafor in poor mobilizing allogeneic stem cell donors: results of a prospective phase II-trial

Author

Hölig, Kristina, Schmidt, Helmuth, Hütter, Gero, Kramer, Michael, Teipel, Raphael, Heidrich, Katharina, Zimmer, Kristin, Heidenreich, Falk, Blechschmidt, Matthias, Torosian, Tigran, Ordemann, Rainer, Kroschinsky, Frank, Rücker-Braun, Elke, Gopsca, Laszlo, Wagner-Drouet, Eva Maria, Oelschlaegel, Uta, Schmidt, Alexander H., Bornhäuser, Martin, Ehninger, Gerhard, and Schetelig, Johannes

Published
2022
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14. Autopsy Study Defines Composition and Dynamics of the HIV-1 Reservoir after Allogeneic Hematopoietic Stem Cell Transplantation with CCR5Δ32/Δ32 Donor Cells

Author

Huyveneers, Laura E. P., primary, Bruns, Anke, additional, Stam, Arjen, additional, Ellerbroek, Pauline, additional, de Jong, Dorien, additional, Nagy, Noémi A., additional, Gumbs, Stephanie B. H., additional, Tesselaar, Kiki, additional, Bosman, Kobus, additional, Salgado, Maria, additional, Hütter, Gero, additional, Brosens, Lodewijk A. A., additional, Kwon, Mi, additional, Diez Martin, Jose, additional, van der Meer, Jan T. M., additional, de Kort, Theun M., additional, Sáez-Cirión, Asier, additional, Schulze zur Wiesch, Julian, additional, Boelens, Jaap Jan, additional, Martinez-Picado, Javier, additional, Kuball, Jürgen H. E., additional, Wensing, Annemarie M. J., additional, and Nijhuis, Monique, additional

Published
2022
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18. Correction to: Salvage treatment with plerixafor in poor mobilizing allogeneic stem cell donors: results of a prospective phase II-trial

Author

Hölig, Kristina, primary, Schmidt, Helmuth, additional, Hütter, Gero, additional, Kramer, Michael, additional, Teipel, Raphael, additional, Heidrich, Katharina, additional, Zimmer, Kristin, additional, Heidenreich, Falk, additional, Blechschmidt, Matthias, additional, Torosian, Tigran, additional, Ordemann, Rainer, additional, Kroschinsky, Frank, additional, Rücker-Braun, Elke, additional, Gopsca, Laszlo, additional, Wagner-Drouet, Eva Maria, additional, Oelschlaegel, Uta, additional, Schmidt, Alexander H., additional, Bornhäuser, Martin, additional, Ehninger, Gerhard, additional, and Schetelig, Johannes, additional

Published
2021
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21. Transplantation of selected or transgenic blood stem cells – a future treatment for HIV/AIDS?

Author

Hütter, Gero, Schneider, Thomas, and Thiel, Eckhard

Subjects
Chemokine receptors -- Health aspects, Highly active antiretroviral therapy -- Dosage and administration, Genetic engineering -- Methods, AIDS treatment -- Genetic aspects, Stem cells -- Transplantation, HIV infection -- Care and treatment -- Genetic aspects, Health
Abstract

Interaction with the chemokine receptor, CCR5, is a necessary precondition for maintaining HIV‐1 infection. Individuals with the CCR5‐delta32 deletion who lack this receptor are highly resistant to infection by the most common forms of HIV‐1. We recently reported on the successful transplantation in an HIV‐1‐positive patient of allogeneic stem cells homozygous for the CCR5‐delta32 allele, which stopped viral replication for more than 27 months without antiretroviral therapy. Here, we report on the results of a meeting regarding the potential implications and future directions of stem cell‐targeted HIV treatments. The meeting drew together an international panel of hematologists, immunologists, HIV specialists and representatives from bone marrow donor registries. The meeting came to an agreement to support further attempts to use CCR5‐delta32 deleted stem cells, for example, prescreened cord blood stem cells, to treat probable HIV‐1‐positive patients with malignancies. Furthermore, improvement of HIV‐1 therapy that interferes with the entry mechanism seems to be a promising approach in HIV‐1‐infected patients with no matching CCR5‐delta32 deleted donor., Introduction Entry of the HIV‐1 into the host cells requires the interaction of the viral envelope with the CD4 surface molecule and certain co‐receptors, predominantly represented by the chemokine receptor, [...]

Published
2009
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26. Salvage treatment with plerixafor in poor mobilizing allogeneic stem cell donors: results of a prospective phase II-trial

Author

Hölig, Kristina, primary, Schmidt, Helmuth, additional, Hütter, Gero, additional, Kramer, Michael, additional, Teipel, Raphael, additional, Heidrich, Katharina, additional, Zimmer, Kristin, additional, Heidenreich, Falk, additional, Blechschmidt, Matthias, additional, Torosian, Tigran, additional, Ordemann, Rainer, additional, Kroschinsky, Frank, additional, Rücker-Braun, Elke, additional, Gopsca, Laszlo, additional, Wagner-Drouet, Eva Maria, additional, Oelschlaegel, Uta, additional, Schmidt, Alexander H., additional, Bornhäuser, Martin, additional, Ehninger, Gerhard, additional, and Schetelig, Johannes, additional

Published
2020
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27. Vulnerability to reservoir reseeding due to high immune activation after allogeneic hematopoietic stem cell transplantation in individuals with HIV-1

Author

Eberhard, Johanna M., primary, Angin, Mathieu, additional, Passaes, Caroline, additional, Salgado, Maria, additional, Monceaux, Valerie, additional, Knops, Elena, additional, Kobbe, Guido, additional, Jensen, Björn, additional, Christopeit, Maximilian, additional, Kröger, Nicolaus, additional, Vandekerckhove, Linos, additional, Badiola, Jon, additional, Bandera, Alessandra, additional, Raj, Kavita, additional, van Lunzen, Jan, additional, Hütter, Gero, additional, Kuball, Jürgen H. E., additional, Martinez-Laperche, Carolina, additional, Balsalobre, Pascual, additional, Kwon, Mi, additional, Díez-Martín, José L., additional, Nijhuis, Monique, additional, Wensing, Annemarie, additional, Martinez-Picado, Javier, additional, Schulze zur Wiesch, Julian, additional, and Sáez-Cirión, Asier, additional

Published
2020
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30. Vulnerability to reservoir reseeding due to high immune activation after allogeneic hematopoietic stem cell transplantation in individuals with HIV-1

Author

CTI Kuball, MS Hematologie, Infection & Immunity, Regenerative Medicine and Stem Cells, Cancer, MMB Research line 3a, MMB opleiding Arts microbioloog, Eberhard, Johanna M., Angin, Mathieu, Passaes, Caroline, Salgado, Maria, Monceaux, Valerie, Knops, Elena, Kobbe, Guido, Jensen, Björn, Christopeit, Maximilian, Kröger, Nicolaus, Vandekerckhove, Linos, Badiola, Jon, Bandera, Alessandra, Raj, Kavita, van Lunzen, Jan, Hütter, Gero, Kuball, Jürgen H.E., Martinez-Laperche, Carolina, Balsalobre, Pascual, Kwon, Mi, Díez-Martín, José L., Nijhuis, Monique, Wensing, Annemarie, Martinez-Picado, Javier, Schulze Zur Wiesch, Julian, Sáez-Cirión, Asier, CTI Kuball, MS Hematologie, Infection & Immunity, Regenerative Medicine and Stem Cells, Cancer, MMB Research line 3a, MMB opleiding Arts microbioloog, Eberhard, Johanna M., Angin, Mathieu, Passaes, Caroline, Salgado, Maria, Monceaux, Valerie, Knops, Elena, Kobbe, Guido, Jensen, Björn, Christopeit, Maximilian, Kröger, Nicolaus, Vandekerckhove, Linos, Badiola, Jon, Bandera, Alessandra, Raj, Kavita, van Lunzen, Jan, Hütter, Gero, Kuball, Jürgen H.E., Martinez-Laperche, Carolina, Balsalobre, Pascual, Kwon, Mi, Díez-Martín, José L., Nijhuis, Monique, Wensing, Annemarie, Martinez-Picado, Javier, Schulze Zur Wiesch, Julian, and Sáez-Cirión, Asier

Published
2020

34. The effect of the CCR5-delta32 deletion on global gene expression considering immune response and inflammation

Author

Hütter Gero, Neumann Martin, Nowak Daniel, Klein Stefan, Klüter Harald, and Hofmann Wolf-K

Subjects
Chemokine, CCR5-delat32, Graft versus host disease, transplantation, Therapeutics. Pharmacology, RM1-950
Abstract

Abstract Background The natural function of the C-C chemokine receptor type 5 (CCR5) is poorly understood. A 32 base pair deletion in the CCR5 gene (CCR5-delta32) located on chromosome 3 results in a non-functional protein. It is supposed that this deletion causes an alteration in T-cell response to inflammation. For example, the presence of the CCR5-delta32 allele in recipients of allografts constitutes as an independent and protective factor associated with a decreased risk of graft-versus-host disease (GVHD) and graft rejection. However, the mechanism of this beneficial effect of the deletion regarding GVHD is unknown. In this survey we searched for a CCR5-delta32 associated regulation of critical genes involved in the immune response and the development of GVHD. Methods We examined CD34+ hematopoietic progenitor cells derived from bone marrow samples from 19 healthy volunteers for the CCR5-delta32 deletion with a genomic PCR using primers flanking the site of the deletion. Results 12 individuals were found to be homozygous for CCR5 WT and 7 carried the CCR5-delta32 deletion heterozygously. Global gene expression analysis led to the identification of 11 differentially regulated genes. Six of them are connected with mechanisms of immune response and control: LRG1, CXCR2, CCRL2, CD6, CD7, WD repeat domain, and CD30L. Conclusions Our data indicate that the CCR5-delta32 mutation may be associated with differential gene expression. Some of these genes are critical for immune response, in the case of CD30L probably protective in terms of GVHD.

Published
2011
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35. High correlation of the proteome patterns in bone marrow and peripheral blood blast cells in patients with acute myeloid leukemia

Author

Thiel Eckhard, Sinha Pranav, Poland Julia, Nowak Daniel, Letsch Anne, Hütter Gero, and Hofmann Wolf-K

Subjects
Medicine
Abstract

Abstract Background When comparing myelogenous blasts from bone marrow and peripheral blood, immunophenotyping usually show a strong correlation of expression of surface antigens. However, it remains to be determined, whether this correlation also exists on the level of protein expression. Method Therefore, we investigated both bone marrow and peripheral blood blast cells from six patients with newly diagnosed acute myeloid leukemia (AML) using conventional two-dimensional electrophoresis in the first dimension and linear polyacrylamide gels (12%) in the second dimension. Proteins were visualized using the silver staining method and image analysis was performed using the PDQuest system. Results For each patient over 80 proteins were evaluated in the sample from peripheral blood and bone marrow. We could demonstrate that the protein expression profile of bone marrow did not significantly differ from the expression patterns of peripheral blast cells. Conclusion The proteome-set of leukemic blast cells from marrow and blood, does not differ substantially when drawn from AML patients with over 80 percent blast cells in both compartments. This indicates that in AML, blasts from peripheral blood samples can be considered suitable for investigations of the proteome using 2D-electrophoresis.

Published
2009
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37. GMP-compliant production of a mesenchymal stem cell based cartilage therapy

Author

Schmidt, Christian, Petters, Oliver, Hütter, Gero, Marquaß, Bastian, Henkelmann, Ralf, and Schulz, Ronny

Subjects
Translation, genetic structures, ddc: 610, MSC based therapy, 610 Medical sciences, Medicine, GMP production
Abstract

Objectives: Due to the limited regeneration potential of articular cartilage, it is still an ambitious clinical challenge to treat cartilage defects with long-term success. Current treatment modalities like microfracture and (matrix-associated) autologous chondrocyte transplantation (M/ACT) have[for full text, please go to the a.m. URL], Deutscher Kongress für Orthopädie und Unfallchirurgie (DKOU 2016)

Published
2016
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38. Plerixafor As a Salvage Regimen to Mobilize Allogeneic Stem Cells in Healthy Volunteers (NCT01954914)

Author

Hoelig, Kristina, primary, Schmidt, Helmuth, additional, Hütter, Gero, additional, Kramer, Michael, additional, Teipel, Raphael, additional, Zimmer, Kristin, additional, Blechschmidt, Matthias, additional, Torosian, Tigran, additional, Ordemann, Rainer, additional, Kroschinsky, Frank, additional, Schmidt, Alexander H., additional, Bornhäuser, Martin, additional, Ehninger, Gerhard, additional, and Schetelig, Johannes, additional

Published
2016
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44. Chemoresistenzassoziierte Veränderungen der Proteinexpression bei Kolon-, Mamma-, Magen-, Pankreaskarzinom und Fibrosarkom mit Hilfe der hochauflösenden zweidimensionalen Elektrophorese im immobilisierten pH-Gradienten

Author

Hütter, Gero, Schadendorf, D., Dietel, M., and Sinha, P.

Subjects
Proteomics, Krebs, 2D-electrophoresis, Chemoresistenz, 610 Medizin, chemoresistance, XH 2550, ddc:610, 2D-Electrophorese, 33 Medizin, Cancer
Abstract

Die Therapie von disseminierten malignen Tumoren durch Chemotherapie allein oder in Kombination mit Bestrahlung oder Hyperthermie führt nur in 20-50% zu einem Ansprechen. Dieser Behandlungserfolg wird häufig dadurch limitiert, daß im Verlauf der Therapie zur Entwicklung einer Chemoresistenz kommt. Ziel der Arbeit war es, eine generelle Analyse der Proteinexpression in der in vitro herbeigeführten Chemoresistenz durchzuführen. Dafür wurden Zellkulturen von Magen-, Kolon-, Pankreas, Mammakarzinom und Fibrosarkom die eine Resistenz gegen Daunorubicin und Mitoxantron besitzen benutzt und mit der zweidimensionalen Gelelektrophorese im immobilisierten pH-Gradienten (pH 4,0-8,0) in der ersten Dimension und einen linearen Polyarcylamidgel (12%) in der zweiten Dimension analysiert. Nach Färbung in Coomassie blau wurde eine rechnergestützte Imageanalyse mit dem PDQuest System durchgeführt. Proteinspots die eine auffällige Änderung zeigten wurden isoliert, und nach enzymatischer Gelhydrolyse mikrosequenziert bzw. durch Massenspektrometrie und anschließender micropore HPLC Analyse identifiziert. Acht Proteine, die in den chemoresistenten Zellinien überexprimiert waren, konnten identifiziert werden: Thioredoxin, Annexin 1; Cofilin, Stratifin(14-3-3sigma), Rho-GDP-Dissoziations Inhibitor, Fettsäurebindendes Protein(E-FABP), Adenin Phosphoribosyl Transferase, und BCSG-1, The therapy of advanced cancer using chemotherapy alone or in combination with radiation or hyperthermia yields an overall response rate of about 20-50%. This success is often marred by the development of resistance to cytostatic drugs. The aim was to study the global analysis of protein expression in the development of chemoresistance in vitro. We therefore used a cell culture model derived from the gastric, colorectal, pancreatic, mamma carcinoma and fibrosarcoma cell line selected to daunorubicin and mitoxantrone. These cell lines were analysed using two-dimensional electrophoresis in immobilized pH-gradients (pH 4.0-8.0) in the first dimension and linear polyacrylamide gels (12%) in the second dimension. After staining with coomassie brilliant blue, image analysis was performed using the PDQuest system. Spots of interest were isolated using preparative two-dimensional electrophoresis and subjected to microsequencing after enzymatic hydrolysis in gel, mass spectrometric data and sequencing of the peptides after their fractionation using microbore HPLC identified. Eight proteins were identified that were overexpressed in chemoresistant cell lines: Thioredoxin, Annexin 1, Cofilin, Stratifin(14-3-3sigma), Rho-GDP-dissoziation Inhibitor, fatty acid binding protein(E-FABP), adenin phosphoribosyl Transferase, and BCSG-1.

Published
2000

47. SNP array analysis of acute promyelocytic leukemia may be of prognostic relevance and identifies a potential high risk group with recurrent deletions on chromosomal subband 1q31.3

Author

Nowak, Daniel, primary, Klaumuenzer, Marion, additional, Hanfstein, Benjamin, additional, Mossner, Maximilian, additional, Nolte, Florian, additional, Nowak, Verena, additional, Oblaender, Julia, additional, Hecht, Anna, additional, Hütter, Gero, additional, Ogawa, Seishi, additional, Kohlmann, Alexander, additional, Haferlach, Claudia, additional, Schlegelberger, Brigitte, additional, Braess, Jan, additional, Seifarth, Wolfgang, additional, Fabarius, Alice, additional, Erben, Philipp, additional, Saussele, Susanne, additional, Müller, Martin C., additional, Reiter, Andreas, additional, Buechner, Thomas, additional, Weiss, Christel, additional, Hofmann, Wolf‐Karsten, additional, and Lengfelder, Eva, additional

Published
2012
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49. Identification of Recurrent Submicroscopic Deletions of chromosome1q31.3 As Possible Molecular Prognostic Markers in Acute Promyelocytic Leukemia (APL)

Author

Nowak, Daniel, primary, Klaumuenzer, Marion, additional, Hanfstein, Benjamin, additional, Mossner, Maximilian, additional, Nolte, Florian, additional, Nowak, Verena, additional, Oblaender, Julia, additional, Hecht, Anna, additional, Hütter, Gero, additional, Ogawa, Seishi, additional, Kohlmann, Alexander, additional, Haferlach, Claudia, additional, Schlegelberger, Brigitte, additional, Braess, Jan, additional, Seifarth, Wolfgang, additional, Fabarius, Alice, additional, Erben, Philipp, additional, Saussele, Susanne, additional, Müller, Martin C., additional, Buechner, Thomas, additional, Weiss, Christel, additional, Hofmann, Wolf-Karsten, additional, and Lengfelder, Eva, additional

Published
2011
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50. Medical importance of Sindbis virus in south-west Germany

Author

Jöst, Hanna, primary, Bürck-Kammerer, Simone, additional, Hütter, Gero, additional, Lattwein, Erik, additional, Lederer, Sabine, additional, Litzba, Nadine, additional, Bock-Hensley, Oswinde, additional, Emmerich, Petra, additional, Günther, Stephan, additional, Becker, Norbert, additional, Niedrig, Matthias, additional, and Schmidt-Chanasit, Jonas, additional

Published
2011
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