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39 results on '"Höhn, Miriam"'

10. Lipoamino bundle LNPs for efficient mRNA transfection of dendritic cells and macrophages show high spleen selectivity

Author

Haase, Franziska, primary, Pöhmerer, Jana, additional, Yazdi, Mina, additional, Grau, Melina, additional, Zeyn, Yanira, additional, Wilk, Ulrich, additional, Burghardt, Tobias, additional, Höhn, Miriam, additional, Hieber, Christoph, additional, Bros, Matthias, additional, Wagner, Ernst, additional, and Berger, Simone, additional

Published
2023
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23. Tuning the Morphological Appearance of Iron(III) Fumarate: Impact on Material Characteristics and Biocompatibility

Author

Hirschle, Patrick, primary, Hirschle, Christian, additional, Böll, Konstantin, additional, Döblinger, Markus, additional, Höhn, Miriam, additional, Tuffnell, Joshua M., additional, Ashling, Christopher W., additional, Keen, David A., additional, Bennett, Thomas D., additional, Rädler, Joachim O., additional, Wagner, Ernst, additional, Peller, Michael, additional, Lächelt, Ulrich, additional, and Wuttke, Stefan, additional

Published
2020
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28. Supramolecular Assembly of Aminoethylene‐Lipopeptide PMO Conjugates into RNA Splice‐Switching Nanomicelles

Author

Kuhn, Jasmin, primary, Klein, Philipp M., additional, Al Danaf, Nader, additional, Nordin, Joel Z., additional, Reinhard, Sören, additional, Loy, Dominik M., additional, Höhn, Miriam, additional, El Andaloussi, Samir, additional, Lamb, Don C., additional, Wagner, Ernst, additional, Aoki, Yoshitsugu, additional, Lehto, Taavi, additional, and Lächelt, Ulrich, additional

Published
2019
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31. Coordinative Binding of Polymers to Metal–Organic Framework Nanoparticles for Control of Interactions at the Biointerface

Author

Zimpel, Andreas, primary, Al Danaf, Nader, additional, Steinborn, Benjamin, additional, Kuhn, Jasmin, additional, Höhn, Miriam, additional, Bauer, Tobias, additional, Hirschle, Patrick, additional, Schrimpf, Waldemar, additional, Engelke, Hanna, additional, Wagner, Ernst, additional, Barz, Matthias, additional, Lamb, Don C., additional, Lächelt, Ulrich, additional, and Wuttke, Stefan, additional

Published
2019
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38. Tuning the Morphological Appearance of Iron(III) Fumarate: Impact on Material Characteristics and Biocompatibility

Author

Hirschle, Patrick, Hirschle, Christian, Böll, Konstantin, Döblinger, Markus, Höhn, Miriam, Tuffnell, Joshua M., Ashling, Christopher W., Keen, David A., Bennett, Thomas D., Rädler, Joachim O., Wagner, Ernst, Peller, Michael, Lächelt, Ulrich, Wuttke, Stefan, Hirschle, Patrick, Hirschle, Christian, Böll, Konstantin, Döblinger, Markus, Höhn, Miriam, Tuffnell, Joshua M., Ashling, Christopher W., Keen, David A., Bennett, Thomas D., Rädler, Joachim O., Wagner, Ernst, Peller, Michael, Lächelt, Ulrich, and Wuttke, Stefan

Abstract

Iron(III) fumarate materials are well suited for biomedical applications as they feature biocompatible building blocks, porosity, chemical functionalizability, and magnetic resonance imaging (MRI) activity. The synthesis of these materials however is difficult to control, and it has been challenging to produce monodisperse particle sizes and morphologies that are required in medical use. Here, we report the optimization of iron(III) fumarate nano- and microparticle synthesis by surfactant-free methods, including room temperature, solvothermal, microwave, and microfluidic conditions. Four variants of iron(III) fumarate with distinct morphologies were isolated and are characterized in detail. Structural characterization shows that all iron(III) fumarate variants exhibit the metal–organic framework (MOF) structure of MIL-88A. Nanoparticles with a diameter of 50 nm were produced, which contain crystalline areas not exceeding 5 nm. Solvent-dependent swelling of the crystalline particles was monitored using in situ X-ray diffraction. Cytotoxicity experiments showed that all iron(III) fumarate variants feature adequate biotolerability and no distinct interference with cellular metabolism at low concentrations. Magnetic resonance relaxivity studies using clinical MRI equipment, on the other hand, proved that the MRI contrast characteristics depend on particle size and morphology. All in all, this study demonstrates the possibility of tuning the morphological appearance of iron(III) fumarate particles and illustrates the importance of optimizing synthesis conditions for the development of new biomedical materials.

39. Receptor-Targeted Dual pH-Triggered Intracellular Protein Transfer.

Author

Lyu M, Yazdi M, Lin Y, Höhn M, Lächelt U, and Wagner E

Subjects
Polyethylene Glycols chemistry, Polyethylene Glycols metabolism, Endosomes metabolism, Hydrogen-Ion Concentration, Melitten metabolism, Cell-Penetrating Peptides chemistry, Cell-Penetrating Peptides metabolism
Abstract

Protein therapeutics are of widespread interest due to their successful performance in the current pharmaceutical and medical fields, even though their broad applications have been hindered by the lack of an efficient intracellular delivery approach. Herein, we fabricated an active-targeted dual pH-responsive delivery system with favorable tumor cell entry augmented by extracellular pH-triggered charge reversal and tumor receptor targeting and pH-controlled endosomal release in a traceless fashion. As a traceable model protein, the enhanced green fluorescent protein (eGFP) bearing a nuclear localization signal was covalently coupled with a pH-labile traceless azidomethyl-methylmaleic anhydride (AzMMMan) linker followed by functionalization with different molar equivalents of two dibenzocyclooctyne-octa-arginine-cysteine (DBCO-R 8 C)-modified moieties: polyethylene glycol (PEG)-GE11 peptide for epidermal growth factor receptor-mediated targeting and melittin for endosomal escape. The cationic melittin domain was masked with tetrahydrophthalic anhydride revertible at mild acidic pH 6.5. At the optimally balanced ratio of functional units, the on-demand charge conversion at tumoral extracellular pH 6.5 in combination with GE11-mediated targeting triggered enhanced electrostatic cellular attraction by the R 8 C cell-penetrating peptides and melittin, as demonstrated by strongly enhanced cellular uptake. Successful endosomal release followed by nuclear localization of the eGFP cargo was obtained by taking advantage of melittin-mediated endosomal escape and rapid traceless release from the AzMMMan linker. The effectiveness of this multifunctional bioresponsive system suggests a promising strategy for delivery of protein drugs toward intracellular targets. A possible therapeutic relevance was indicated by an example of cytosolic delivery of cytochrome c initiating the apoptosis pathway to kill cancer cells.

Published
2024
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