1. In vitro biological response of human osteoblasts in 3D chitosan sponges with controlled degree of deacetylation and molecular weight
- Author
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Mousumi Sukul, Håvard J. Haugen, João F. Mano, Priyanka Sahariah, Janne E. Reseland, Hélène L. Lauzon, João Borges, and Már Másson
- Subjects
Vascular Endothelial Growth Factor A ,Bone Regeneration ,Polymers and Plastics ,Primary Cell Culture ,Gene Expression ,02 engineering and technology ,010402 general chemistry ,01 natural sciences ,Models, Biological ,Chitosan ,chemistry.chemical_compound ,Osteoprotegerin ,Materials Chemistry ,medicine ,Humans ,Osteopontin ,Bone regeneration ,Chemokine CCL2 ,Adaptor Proteins, Signal Transducing ,Cell Proliferation ,Osteoblasts ,biology ,Molecular Structure ,Interleukin-6 ,Monocyte ,Organic Chemistry ,Acetylation ,021001 nanoscience & nanotechnology ,Alkaline Phosphatase ,In vitro ,0104 chemical sciences ,Cell biology ,Molecular Weight ,medicine.anatomical_structure ,chemistry ,Delayed-Action Preparations ,biology.protein ,Alkaline phosphatase ,Sclerostin ,Intercellular Signaling Peptides and Proteins ,0210 nano-technology ,Biomarkers - Abstract
We have studied the effect of chitosan sponges, produced from chitosan batches with distinct degree of deacetylation (DDA) and molecular weight (Mw), on the adhesion, growth and differentiation of primary human osteoblasts with an aim to offer a suitable tool for guided bone regeneration. All the chitosan sponges revealed similar microstructure, irrespective of the DDA (58, 73, 82, 88, and 91 %) and Mw (749, 547, 263, 215, and 170 kDa, respectively). Cell spreading was higher on sponges having a higher DDA. Higher DDA induced a more pronounced increase in alkaline phosphatase activity, osteopontin (OPN), vascular endothelial growth factor-A (VEGF), interleukin-6 (IL-6), and reduction in monocyte chemoattractant protein-1 (MCP-1), sclerostin (SOST) and dickkopf related protein-1 as compared to lower DDA. Lower DDA induced the increased secretion of osteoprotegerin and SOST as compared to higher DDA. The combination of higher DDA and Mw induced an increased secretion of VEGF and IL-6, however reduced the secretion of OPN as compared to chitosan with similar DDA but with lower Mw. In summary, the variations in cellular responses to the different chitosan sponges indicate a potential for individual tailoring of desired responses in guided bone regeneration.
- Published
- 2020