Author: "Hélène Bracq-Dieye" - Searchworks@Jio Institute Digital Library Search Results

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1. Genome-Based Characterization of Listeria monocytogenes, Costa Rica

Author: María Giralt-Zúñiga, Mauricio Redondo-Solano, Alexandra Moura, Nathalie Tessaud-Rita, Hélène Bracq-Dieye, Guillaume Vales, Pierre Thouvenot, Alexandre Leclercq, Carolina Chaves-Ulate, Kattia Núñez-Montero, Rossy Guillén-Watson, Olga Rivas-Solano, Grettel Chanto-Chacón, Francisco Duarte-Martínez, Vanessa Soto-Blanco, Javier Pizarro-Cerdá, and Marc Lecuit
Subjects: Listeria monocytogenes, bacteria, food safety, whole-genome sequencing, pathogen surveillance, outbreak detection, Medicine, Infectious and parasitic diseases, RC109-216
Abstract: Genomic data on the foodborne pathogen Listeria monocytogenes from Central America are scarce. We analyzed 92 isolates collected during 2009–2019 from different regions in Costa Rica, compared those to publicly available genomes, and identified unrecognized outbreaks. Our findings suggest mandatory reporting of listeriosis in Costa Rica would improve pathogen surveillance.
Published: 2023
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2. Phenotypic and genotypic antimicrobial resistance of Listeria monocytogenes: an observational study in FranceResearch in context

Author: Alexandra Moura, Alexandre Leclercq, Guillaume Vales, Nathalie Tessaud-Rita, Hélène Bracq-Dieye, Pierre Thouvenot, Yoann Madec, Caroline Charlier, and Marc Lecuit
Subjects: Listeriosis, Antimicrobial resistance, Genome, Phenotype, Prediction, Public aspects of medicine, RA1-1270
Abstract: Summary: Background: Large-scale studies are needed to clarify antimicrobial resistance in the foodborne pathogen Listeria monocytogenes (Lm) and the effectiveness of listeriosis treatment options. Here we examined the antimicrobial resistance patterns in Lm over time and assessed genotype-phenotype concordances. Methods: We analyzed 5339 Lm isolates (2908 clinical and 2431 food isolates) collected in France and overseas territories, between 2012 and 2019. Whole genome sequencing was performed for all isolates and antimicrobial resistance profiles inferred from draft assemblies. Antimicrobial susceptibility towards 22 antimicrobials was determined for all clinical isolates, and in food isolates with acquired resistance genes. Findings: All tested isolates were resistant to at least 3 different classes of antimicrobials, consistent with Lm intrinsic traits. Acquired antimicrobial resistance in Lm was rare (2.23% isolates) and more prevalent in food (mainly lineage II) compared to clinical isolates (mainly lineage I) (3.74% vs 0.98%, p 99%), except for ciprofloxacin. Acquired antimicrobial phenotypes were towards tetracyclines (mostly due to tetM), trimethoprim (dfrD), lincosamides (lnuG), macrolides (ermB, mphB) and phenicols (fexA). Interpretation: The reference treatment for listeriosis (aminopenicillins/aminoglycosides) remains effective, with no acquired resistance observed. Continuous surveillance of antimicrobial resistance in clinical and food isolates is crucial to detect the emergence of novel resistance. Funding: Institut Pasteur, INSERM, Santé Publique France, Investissement d’Avenir program Laboratoire d’Excellence ‘Integrative Biology of Emerging Infectious Diseases’ (ANR-10-LABX-62-IBEID).
Published: 2024
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3. Hypervirulent Listeria monocytogenes clones’ adaption to mammalian gut accounts for their association with dairy products

Author: Mylène M. Maury, Hélène Bracq-Dieye, Lei Huang, Guillaume Vales, Morgane Lavina, Pierre Thouvenot, Olivier Disson, Alexandre Leclercq, Sylvain Brisse, and Marc Lecuit
Subjects: Science
Abstract: Here, Maury et al. show that hypervirulent Listeria monocytogenes (Lm) clones associated to dairy products exhibit higher adaptation to the mammalian gut environment, while hypovirulent clones persist in food processing environment, suggesting a relationship between Lm pathogenic potential and niche adaptation.
Published: 2019
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4. Real-Time Whole-Genome Sequencing for Surveillance of Listeria monocytogenes, France

Author: Alexandra Moura, Mathieu Tourdjman, Alexandre Leclercq, Estelle Hamelin, Edith Laurent, Nathalie Fredriksen, Dieter Van Cauteren, Hélène Bracq-Dieye, Pierre Thouvenot, Guillaume Vales, Nathalie Tessaud-Rita, Mylène M. Maury, Andreea Alexandru, Alexis Criscuolo, Emmanuel Quevillon, Marie-Pierre Donguy, Vincent Enouf, Henriette de Valk, Sylvain Brisse, and Marc Lecuit
Subjects: Listeria monocytogenes, bacteria, whole-genome sequencing, high-throughput DNA sequencing, molecular typing, public health, Medicine, Infectious and parasitic diseases, RC109-216
Abstract: During 2015–2016, we evaluated the performance of whole-genome sequencing (WGS) as a routine typing tool. Its added value for microbiological and epidemiologic surveillance of listeriosis was compared with that for pulsed-field gel electrophoresis (PFGE), the current standard method. A total of 2,743 Listeria monocytogenes isolates collected as part of routine surveillance were characterized in parallel by PFGE and core genome multilocus sequence typing (cgMLST) extracted from WGS. We investigated PFGE and cgMLST clusters containing human isolates. Discrimination of isolates was significantly higher by cgMLST than by PFGE (p
Published: 2017
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5. Author Correction: Hypervirulent Listeria monocytogenes clones’ adaptation to mammalian gut accounts for their association with dairy products

Author: Mylène M. Maury, Hélène Bracq-Dieye, Lei Huang, Guillaume Vales, Morgane Lavina, Pierre Thouvenot, Olivier Disson, Alexandre Leclercq, Sylvain Brisse, and Marc Lecuit
Subjects: Science
Abstract: An amendment to this paper has been published and can be accessed via a link at the top of the paper.
Published: 2019
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6. Phenotypic and Genotypic Antimicrobial Resistance of Listeria Monocytogenes in France

Author: Alexandra Moura, Alexandre Leclercq, Guillaume Valès, Nathalie Tessaud-Rita, Hélène Bracq-Dieye, Pierre Thouvenot, Yoann Madec, Caroline Charlier, and Marc Lecuit
Published: 2023
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7. Genomic characterization of Listeria spp. isolated from tonsils, udder and feces of domestic dairy ruminants in Spain

Author: Carla Palacios-Gorba, Alexandra Moura, Yuval Markovich, Nathalie Tessaud-Rita, Ángel Gómez-Martín, Hélène Bracq-Dieye, Jesús Gomis, Guillaume Vales, María Pastor-Martín, Pierre Thouvenot, Carles Escrig, Alexandre Leclercq, Marc Lecuit, Juan J. Quereda, Universidad Cardenal Herrera-CEU (CEU-UCH), Biologie des Infections - Biology of Infection, Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité), Centre National de Référence Listeria - National Reference Center Listeria (CNR), Centre collaborateur de l'OMS Listeria / WHO Collaborating Centre Listeria (CC-OMS / WHO-CC), Institut Pasteur [Paris] (IP)-Organisation Mondiale de la Santé / World Health Organization Office (OMS / WHO)-Université Paris Cité (UPCité), Generalitat Valenciana, CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Imagine - Institut des maladies génétiques (IHU) (Imagine - U1163), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité), and This work was supported by Generalitat Valenciana (Project reference AICO/2021/278) (JJQ), the Spanish Ministry of Science and Innovation (Project references PID2019-110764RA-I00/AEI/10.13039/501100011033 (JJQ) and PID2020-119462RA-I00/AEI/10.13039/501100011033 (AGM)) and Universidad CEU Cardenal Herrera Programa INDI 20/40 (JJQ), Institut Pasteur, Inserm, and Santé Publique France (ML). J.J. Quereda is supported by a 'Ramón y Cajal' contract of the Spanish Ministry of Science, Innovation, and Universities (RYC-2018-024985-I). C. Palacios-Gorba and Yuval Markovich are supported by a Predoctoral contract from the Universidad Cardenal Herrera-CEU.
Subjects: [SDV]Life Sciences [q-bio], Immunology, ruminant, Microbiology, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, Infectious Diseases, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, udder, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, tonsil, dairy, Listeria spp, WGS, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract: International audience; Two species of Listeria are pathogenic, Listeria monocytogenes and Listeria ivanovii. Although studies have shown that dairy ruminants shed Listeria spp. in feces, there is little information about ruminants that do not shed Listeria spp. in their feces but asymptomatically carry them in organs. We evidence that ruminants can asymptomatically carry L. ivanovii in udders and L. monocytogenes and L. ivanovii in tonsils without fecal shedding. Whole-genome sequence of L. monocytogenes and L. ivanovii contained known core genes involved in virulence and antibiotic resistance. This work highlights tonsils and udders as a Listeria intra-host site of colonization.
Published: 2022
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8. Listeria valentina sp. nov., isolated from a water trough and the faeces of healthy sheep

Author: Alexandra Moura, Ángel Gómez-Martín, Guillaume Vales, Nathalie Tessaud-Rita, Ángel García-Muñoz, Marc Lecuit, Juan J. Quereda, Alexandre Leclercq, Hélène Bracq-Dieye, Pierre Thouvenot, Universidad Cardenal Herrera-CEU (CEU-UCH), Biologie des Infections - Biology of Infection, Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre National de Référence Listeria - National Reference Center Listeria (CNRL), Centre collaborateur de l'OMS Listeria / WHO Collaborating Centre Listeria (CC-OMS / WHO-CC), Institut Pasteur [Paris] (IP)-Organisation Mondiale de la Santé / World Health Organization Office (OMS / WHO), Service des Maladies infectieuses et tropicales [CHU Necker], CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), This work was supported by Generalitat Valenciana (Project reference GV/2018/A/183) and by the Spanish Ministry of Science and Innovation (Project reference PID2019-110764RA-I00), Institut Pasteur, Inserm and Santé Publique France. J.J. Quereda is supported by a ‘Ramón y Cajal’ contract of the Spanish Ministry of Science, Innovation and Universities (RYC-2018–024985-I)., DIAKITE, andrée, Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), and Institut Pasteur [Paris]-Organisation Mondiale de la Santé / World Health Organization Office (OMS / WHO)
Subjects: POCP, [SDV]Life Sciences [q-bio], Context (language use), Microbiology, new taxa, 03 medical and health sciences, Monophyly, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, Clade, [SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology, isDDH, Ecology, Evolution, Behavior and Systematics, 030304 developmental biology, Whole genome sequencing, whole genome sequencing, 0303 health sciences, [SDV.MHEP] Life Sciences [q-bio]/Human health and pathology, biology, Phylogenetic tree, AAI, 030306 microbiology, General Medicine, isDDH Subject category: International Journal of Systematic and Evolutionary Microbiology Designation of Neotype Strains Subject: New Taxa -Firmicutes and Related Organisms, 16S ribosomal RNA, biology.organism_classification, [SDV] Life Sciences [q-bio], [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, Taxon, ANI, [SDV.MHEP.MI] Life Sciences [q-bio]/Human health and pathology/Infectious diseases, Listeria, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract: In the context of a study on the occurrence of Listeria species in an animal farm environment in Valencia, Spain, six Listeria -like isolates could not be assigned to any known species. Phylogenetic analysis based on the 16S rRNA gene and on 231 Listeria core genes grouped these isolates in a monophyletic clade within the genus Listeria , with highest similarity to Listeria thailandensis . Whole-genome sequence analyses based on in silico DNA–DNA hybridization, the average nucleotide blast and the pairwise amino acid identities against all currently known Listeria species confirmed that these isolates constituted a new taxon within the genus Listeria . Phenotypically, these isolates differed from other Listeria species mainly by the production of acid from inositol, the absence of acidification in presence of methyl α-d-glucoside, and the absence of α-mannosidase and nitrate reductase activities. The name Listeria valentina sp. nov. is proposed for this novel species, and the type strain is CLIP 2019/00642T (=CIP 111799T=DSM 110544T).
Published: 2020
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9. Listeria-associated lymphadenitis: a series of 11 consecutive cases and review of the literature

Author: Mathieu Blot, Olivier Disson, Alexandre Leclercq, Alexandra Moura, Hélène Bracq-Dieye, Pierre Thouvenot, Guillaume Valès, Barbara Burroni, Audrey Lupo, Marc Lecuit, Caroline Charlier, Centre National de Référence Listeria - National Reference Center Listeria (CNRL), Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre collaborateur de l'OMS Listeria / WHO Collaborating Centre Listeria (CC-OMS / WHO-CC), Institut Pasteur [Paris] (IP)-Organisation Mondiale de la Santé / World Health Organization Office (OMS / WHO), Service des Maladies infectieuses et tropicales [CHU Necker], CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Imagine - Institut des maladies génétiques (IHU) (Imagine - U1163), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité), Biologie des Infections - Biology of Infection, Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité), UFR Médecine [Santé] - Université Paris Cité (UFR Médecine UPCité), Université Paris Cité (UPCité), This work was funded by Institut Pasteur, Institut national de la santé et de la recherche médicale (INSERM), and Santé Publique France., We thank David Hardy (Experimental Neuropathology Unit, Institut Pasteur Paris) for section preparations. We thank Dr. Marie-Albane Bensussan (University Hospital, Amiens), Drs. Stéphane Bland and Helene Petitprez (Annecy-Genevois Hospital), Karine Aubry (University Hospital, Limoges), Dr. Burroni Barbara (Cochin University Hospital, Paris), Dr. Jean Puyhardy (HIA Legouest Hospital, Metz), and Dr. Barrans Alain (Bassin de Thau Hospital, Sete)., DIAKITE, andrée, and Université Paris Cité - UFR Médecine [Santé] (UPCité UFR Médecine)
Subjects: adenitis, [SDV.MHEP] Life Sciences [q-bio]/Human health and pathology, Brief Report, [SDV]Life Sciences [q-bio], Listeria monocytogenes, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, [SDV] Life Sciences [q-bio], AcademicSubjects/MED00290, Infectious Diseases, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, Oncology, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, [SDV.MHEP.MI] Life Sciences [q-bio]/Human health and pathology/Infectious diseases, cancer, [SDV.MP.BAC] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, [SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract: We studied 11 cases of culture-proven Listeria-associated lymphadenitis reported to the French National Reference Center for Listeria from 1994 to 2019 and 8 additional published cases. Listeria-associated lymphadenitis is rare, but it is associated with a mortality as high as for invasive listeriosis, and it is frequently diagnosed with concomitant neoplasia., We studied 11 cases of Listeria-associated lymphadenitis reported to the French Reference Center for Listeria, and 8 published cases. Listeria-associated lymphadenitis is rare but associated with a mortality as high as for invasive listeriosis, and frequently diagnosed with concomitant neoplasia.
Published: 2022
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10. Ruminant-associated Listeria monocytogenes isolates belong preferentially to dairy-related hypervirulent clones: a longitudinal study in 19 farms

Author: Pierre Thouvenot, Guillaume Vales, Juan J. Quereda, Empar García-Roselló, María L. Mocé, Ángel Gómez-Martín, Nathalie Tessaud-Rita, Alexandre Leclercq, E. Jiménez-Trigos, Marc Lecuit, Hélène Bracq-Dieye, Ángel García-Muñoz, Alexandra Moura, Jesús Gomis, and Carla Palacios-Gorba
Subjects: Genetic diversity, Veterinary medicine, Longitudinal study, biology, Listeria monocytogenes, Ruminant, Listeria, medicine, biology.organism_classification, medicine.disease_cause, Pathogen, Asymptomatic carrier, Feces
Abstract: The increasing prevalence of Listeria monocytogenes infections is a public health issue. Although studies have shown that ruminants constitute reservoirs of this foodborne pathogen, little is known about its epidemiology and genetic diversity within ruminant farms. Here we conducted a large-scale genomic and epidemiologic longitudinal study of Listeria spp. in dairy ruminants and their environments, comprising 19 farms monitored for three consecutive seasons (N=3251 samples). L. innocua was the most prevalent Listeria spp, followed by L. monocytogenes. L. monocytogenes was detected in 52.6% of farms (prevalence in feces samples 3.8%, in farm environment samples 2.5%) and more frequently in cattle (4.1%) and sheep (4.5%) than in goat farms (0.2%). Lineage I accounted for 69% of L. monocytogenes isolates. Among animal samples, the most prevalent sublineages (SL) and clonal complexes (CC) were SL1/CC1, SL219/CC4, SL26/CC26 and SL87/CC87, whereas SL666/CC666 was prevalent in environmental samples. 61 different L. monocytogenes CTs (cgMLST sequence types) were found, 17 of them (27.9%) common to different animals and/or surfaces within the same farms. L. monocytogenes prevalence was not affected by farm hygiene but by season: the overall prevalence of L. monocytogenes in cattle farms was higher during winter, and in sheep farms was higher during winter and spring. Cows in their second lactation had a higher probability of L. monocytogenes fecal shedding than other lactating cows. This study highlights that dairy farms constitute a reservoir for hypervirulent L. monocytogenes and the importance of continuous animal surveillance to reduce the burden of human listeriosis.IMPORTANCEListeria monocytogenes is a bacterial pathogen responsible for listeriosis, the foodborne disease with the highest hospitalization and case-fatality rate. Despite increasing evidence that dairy products and ruminant farms are important reservoirs of L. monocytogenes, little is known about the epidemiology and genetic diversity of Listeria spp. within dairy ruminant farms. We report the largest Listeria spp. longitudinal study in individual domestic animals, and the first using whole-genome sequencing for a deep isolate characterization. Here, we show that domestic ruminants can be asymptomatic carriers of pathogenic Listeria, that L. monocytogenes fecal shedding is often intermittent, and that hypervirulent L. monocytogenes clones are overrepresented in dairy farms. Moreover, we uncover the effect of seasons and lactation number on the prevalence of L. monocytogenes in ruminants. Our study highlights the need for Listeria spp. monitoring in farm animals to control the spread of hypervirulent L. monocytogenes and reduce the burden of human listeriosis.
Published: 2021
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11. Neonatal Listeriosis Presentation and Outcome: A Prospective Study of 189 Cases

Author: Alexandra Moura, Pierre Thouvenot, Elodie Perrodeau, Elsa Kermorvant-Duchemin, Hélène Bracq-Dieye, Alexandre Leclercq, Marc Lecuit, Guillaume Vales, Mylène M. Maury, Philippe Ravaud, Caroline Charlier, Centre National de Référence Listeria - National Reference Center Listeria (CNRL), Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre collaborateur de l'OMS Listeria / WHO Collaborating Centre Listeria (CC-OMS / WHO-CC), Institut Pasteur [Paris] (IP)-Organisation Mondiale de la Santé / World Health Organization Office (OMS / WHO), Biologie des Infections - Biology of Infection, Université Paris Cité (UPCité), CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Service des Maladies infectieuses et tropicales [CHU Necker], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Imagine - Institut des maladies génétiques (IHU) (Imagine - U1163), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité), Université de Paris (1896-1970), Equipe 5 : METHODS - Méthodes de l’évaluation thérapeutique des maladies chroniques (CRESS - U1153), Centre de Recherche Épidémiologie et Statistique Sorbonne Paris Cité (CRESS (U1153 / UMR_A_1125 / UMR_S_1153)), Conservatoire National des Arts et Métiers [CNAM] (CNAM), HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Conservatoire National des Arts et Métiers [CNAM] (CNAM), HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), This work was supported by the Institut Pasteur, Inserm, Santé Publique France, and Programme Hospitalier Recherche Clinique, Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Pasteur [Paris]-Organisation Mondiale de la Santé / World Health Organization Office (OMS / WHO), Université Paris Cité (UPC), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPC), HESAM Université (HESAM)-HESAM Université (HESAM)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPC)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Conservatoire National des Arts et Métiers [CNAM] (CNAM), and HESAM Université (HESAM)-HESAM Université (HESAM)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPC)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)
Subjects: Microbiology (medical), Pediatrics, medicine.medical_specialty, Resuscitation, [SDV]Life Sciences [q-bio], Context (language use), Gestational Age, medicine.disease_cause, Infant, Newborn, Diseases, Listeria monocytogenes, Informed consent, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, Pregnancy, medicine, Humans, Listeriosis, Prospective Studies, Prospective cohort study, amoxicillin, business.industry, neonatal listeriosis, Infant, Newborn, Gestational age, meningitis, Infant, Institutional review board, medicine.disease, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, Infectious Diseases, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, outcome, Gestation, Female, business, Meningitis, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology, Neonatal Listeriosis
Abstract: Background Listeriosis is caused by the foodborne pathogen Listeria monocytogenes. It can present as a maternal–neonatal infection. We implemented a nationwide prospective cohort and analyzed the features of neonatal listeriosis. Methods We studied all neonates born alive from mothers with microbiologically proven maternal–neonatal listeriosis enrolled from November 2009 to December 2017. We analyzed presentation, neonatal outcome at discharge, and predictors of severe presentation and outcome. Results We studied 189 infants; 133 of 189 (70%) had abnormal clinical status at birth, including acute respiratory distress in 106 of 189 (56%). There were 132 of 189 (70%) infants who developed early-onset listeriosis and 12 of 189 (6%) who developed late-onset listeriosis; all presented with acute meningitis. There were 17 of 189 (9%) infants who had major adverse outcomes: 3%, (5 of 189) death; 6% (12 of 189), severe brain injury; and 2% (3 of 189), severe bronchopulmonary dysplasia. Fifteen of 17 infants were born Conclusions Antenatal maternal antimicrobial treatment is associated with reduced neonatal listeriosis severity, justifying the prescription of preemptive maternal antimicrobial therapy when maternal–fetal listeriosis is suspected. Neonatal outcome is better than reported earlier, and its major determinant is gestational age at birth. Clinical Trials Registration NCT01520597.
Published: 2021
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12. Cutaneous listeriosis, a case series of 16 consecutive patients over 25 years

Author: Alexandra Moura, Alexandre Leclercq, Caroline Charlier, Pierre Thouvenot, Mylène M. Maury, Guillaume Vales, Marc Lecuit, Benoit Pilmis, Hélène Bracq-Dieye, Centre National de Référence Listeria - National Reference Center Listeria (CNRL), Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre collaborateur de l'OMS Listeria / WHO Collaborating Centre Listeria (CC-OMS / WHO-CC), Institut Pasteur [Paris] (IP)-Organisation Mondiale de la Santé / World Health Organization Office (OMS / WHO), Service des Maladies infectieuses et tropicales [CHU Necker], CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Biologie des Infections - Biology of Infection, Université Paris Descartes - Paris 5 (UPD5), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Pasteur [Paris]-Organisation Mondiale de la Santé / World Health Organization Office (OMS / WHO), and DIAKITE, andrée
Subjects: Microbiology (medical), medicine.medical_specialty, skin, [SDV]Life Sciences [q-bio], Cutaneous listeriosis, Biology, medicine.disease_cause, 03 medical and health sciences, Listeria monocytogenes, listeriosis, Risk Factors, medicine, Humans, Listeria monocytogenes Word count: 866, ComputingMilieux_MISCELLANEOUS, 030304 developmental biology, 0303 health sciences, [SDV.MHEP] Life Sciences [q-bio]/Human health and pathology, 030306 microbiology, [SDV.MHEP.DERM] Life Sciences [q-bio]/Human health and pathology/Dermatology, Dermatology, 3. Good health, [SDV] Life Sciences [q-bio], Infectious Diseases, England, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology, [SDV.MHEP.DERM]Life Sciences [q-bio]/Human health and pathology/Dermatology
Abstract: International audience
Published: 2020
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13. Author Correction: Hypervirulent Listeria monocytogenes clones’ adaptation to mammalian gut accounts for their association with dairy products

Author: Morgane Lavina, Mylène M. Maury, Pierre Thouvenot, Olivier Disson, Guillaume Vales, Alexandre Leclercq, Marc Lecuit, Sylvain Brisse, Hélène Bracq-Dieye, and Lei Huang
Subjects: Food Handling, Science, General Physics and Astronomy, Food Contamination, Biology, medicine.disease_cause, General Biochemistry, Genetics and Molecular Biology, Microbial ecology, Foodborne Diseases, 03 medical and health sciences, Listeria monocytogenes, Bacterial genetics, Drug Resistance, Bacterial, medicine, Animals, Humans, lcsh:Science, Author Correction, Ecosystem, 030304 developmental biology, Genetics, 0303 health sciences, Multidisciplinary, Virulence, 030306 microbiology, General Chemistry, Adaptation, Physiological, Clone Cells, Intestines, Meat Products, Biofilms, Anti-Infective Agents, Local, lcsh:Q, Dairy Products, Adaptation, Pathogens, Benzalkonium Compounds
Abstract: Listeria monocytogenes (Lm) is a major human and animal foodborne pathogen. Here we show that hypervirulent Lm clones, particularly CC1, are strongly associated with dairy products, whereas hypovirulent clones, CC9 and CC121, are associated with meat products. Clone adaptation to distinct ecological niches and/or different food products contamination routes may account for this uneven distribution. Indeed, hypervirulent clones colonize better the intestinal lumen and invade more intestinal tissues than hypovirulent ones, reflecting their adaption to host environment. Conversely, hypovirulent clones are adapted to food processing environments, with a higher prevalence of stress resistance and benzalkonium chloride tolerance genes and a higher survival and biofilm formation capacity in presence of sub-lethal benzalkonium chloride concentrations. Lm virulence heterogeneity therefore reflects the diversity of the ecological niches in which it evolves. These results also have important public health implications and may help in reducing food contamination and improving food consumption recommendations to at-risk populations.
Published: 2019

14. Listeria monocytogenes-associated endovascular infections: A study of 71 consecutive cases

Author: Guillaume Vales, Grégory Jouvion, Caroline Charlier, Gabrielle Coulpier, Olivier Disson, Marc Lecuit, Benoit Pilmis, Alexandre Leclercq, Pierre Thouvenot, Mylène M. Maury, Michka Shoai-Tehrani, Hélène Bracq-Dieye, Olivier Robineau, Laboratoire des Listeria, Centre National de Référence des Listeria, et Centre collaborateur de l’OMS pour la listériose d’origine alimentaire (CNR - CCOMS), Institut Pasteur [Paris], Service des Maladies infectieuses et tropicales [CHU Necker], CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Centre collaborateur de l'OMS Listeria / WHO Collaborating Centre Listeria (CC-OMS / WHO-CC), Institut Pasteur [Paris]-Organisation Mondiale de la Santé / World Health Organization Office (OMS / WHO), Centre National de Référence Listeria - National Reference Center Listeria (CNRL), Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre Hospitalier de Tourcoing, Biologie des Infections - Biology of Infection, Neuropathologie expérimentale - Experimental neuropathology, Institut Pasteur [Paris]-Université Paris Descartes - Paris 5 (UPD5), Université Paris Descartes - Paris 5 (UPD5), This study received financial support from Institut Pasteur, Inserm and Santé Publique France., We thank Dr. Isabelle Serre (Gui Chauliac University Hospital, Montpellier, France), Dr. Valérie Hervieu (Edouard Herriot University Hospital, Lyon, France), Dr. Beatrice Luciani-Laillou (Périgueux, France) and Dr. Luc Marcellin (Hautepierre University Hospital, Strasbourg, France), David Hardy and Magali Tichit (Experimental Neuropathology Unit, Institut Pasteur, Paris) for their contribution to histopathology studies, as well as Giulia Nigro (Molecular Microbial Pathologenesis Unit, Institut Pasteur, Paris)., Institut Pasteur [Paris] (IP), Institut Pasteur [Paris] (IP)-Organisation Mondiale de la Santé / World Health Organization Office (OMS / WHO), Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Pasteur [Paris] (IP)-Université Paris Descartes - Paris 5 (UPD5), and DIAKITE, andrée
Subjects: 0301 basic medicine, Male, medicine.medical_treatment, [SDV]Life Sciences [q-bio], Prosthesis, medicine.disease_cause, Intracardiac injection, 0302 clinical medicine, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, Risk Factors, Medicine, Listeriosis, 030212 general & internal medicine, Hospital Mortality, Aged, 80 and over, [SDV.MHEP] Life Sciences [q-bio]/Human health and pathology, Endocarditis, Middle Aged, 3. Good health, [SDV] Life Sciences [q-bio], Infectious Diseases, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, Heart Valve Prosthesis, Epidemiological Monitoring, [SDV.MHEP.MI] Life Sciences [q-bio]/Human health and pathology/Infectious diseases, Female, France, Microbiology (medical), medicine.medical_specialty, 030106 microbiology, 03 medical and health sciences, Aneurysm, Listeria monocytogenes, Humans, [SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology, Aged, Retrospective Studies, business.industry, Retrospective cohort study, Endocarditis, Bacterial, medicine.disease, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, Surgery, Heart failure, Concomitant, [SDV.MP.BAC] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, business, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract: International audience; Background: Listeria monocytogenes-associated endovascular infections are not well characterized. Methods: Retrospective study of 71 culture-proven cases reported to the French National Reference Center for Listeria from 1993 to 2018. Results: Seventy-one cases were identified: 42 with vascular aneurysms/prosthetic infections, 27 with endocarditis, 2 with both. Fifty-eight were men (82%); median age was 75 years [46-92]; 93% reported co-morbidities (66/71), including 50% with immunosuppressive conditions. Vascular infections consisted of infected aneurysms (68%) or prosthetic graft infections (32%); vascular rupture was reported in 25/42 (60%). Tissue samples grew L. monocytogenes in 98% (43/44) and blood cultures in 64% (27/42). Endo-carditis cases involved prosthetic or native valves or intracardiac devices in respectively 62% (18/29), 28% (8/29) and 10% (3/29). Infected valves were aortic (62%, 16/26), mitral (31%, 8/26) or both (8%, 2/26); 38% patients required surgery; 45% displayed heart failure; 17% had concomitant neurolisteriosis. In-hospital mortality in vascular infections was 12% (5/42) and 41% (12/29) for Lm-associated endocarditis. Conclusions: Endovascular listeriosis is a rare but severe infection. It manifests as vascular infections and endocarditis, mostly in older patients with vascular or cardiac valve prosthetic devices and co-morbidities. Mortality in Lm-associated endocarditis is twice higher than with other pathogens, requiring prompt recognition and treatment.
Published: 2019
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15. Listeria monocytogenes-associated respiratory infections: a study of 38 consecutive cases

Author: M. Morgand, Mylène M. Maury, Caroline Charlier, Alexandre Leclercq, Pierre Thouvenot, Guillaume Vales, Hélène Bracq-Dieye, Marc Lecuit, Service des Maladies infectieuses et tropicales [CHU Necker], CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Centre National de Référence Listeria - National Reference Center Listeria (CNRL), Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre collaborateur de l'OMS Listeria / WHO Collaborating Centre Listeria (CC-OMS / WHO-CC), Institut Pasteur [Paris]-Organisation Mondiale de la Santé / World Health Organization Office (OMS / WHO), Biologie des Infections - Biology of Infection, Université Paris Descartes - Paris 5 (UPD5), Funding was received from the Institut Pasteur, Santé Publique, France., Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Pasteur [Paris] (IP)-Organisation Mondiale de la Santé / World Health Organization Office (OMS / WHO), and DIAKITE, andrée
Subjects: Lung Diseases, Male, 0301 basic medicine, MESH: Respiratory Tract Infections/epidemiology, MESH: Listeria monocytogenes/isolation & purification, MESH: Empyema, Pleural/microbiology, Pleural effusion, MESH: Empyema, Pleural/etiology, medicine.medical_treatment, [SDV]Life Sciences [q-bio], MESH: Empyema, Pleural/drug therapy, MESH: Listeriosis/epidemiology, MESH: Listeriosis/drug therapy, MESH: Aged, 80 and over, listeriosis, pleural effusion, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, MESH: Sepsis/etiology, MESH: Anti-Bacterial Agents/therapeutic use, Respiratory Tract Infections, MESH: Respiratory Tract Infections/etiology, Aged, 80 and over, MESH: Aged, Pleuropneumonia, [SDV.MHEP] Life Sciences [q-bio]/Human health and pathology, MESH: Middle Aged, Respiratory infection, Immunosuppression, General Medicine, Middle Aged, MESH: Pneumonia, Bacterial/drug therapy, MESH: Pneumonia, Bacterial/etiology, MESH: Sepsis/epidemiology, Anti-Bacterial Agents, 3. Good health, [SDV] Life Sciences [q-bio], Infectious Diseases, medicine.anatomical_structure, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, MESH: Lung Diseases/microbiology, [SDV.MHEP.MI] Life Sciences [q-bio]/Human health and pathology/Infectious diseases, MESH: Pneumonia, Bacterial/microbiology, Female, MESH: Empyema, Pleural/epidemiology, medicine.drug, Adult, Microbiology (medical), MESH: Pleuropneumonia/microbiology, MESH: Pneumonia, Bacterial/epidemiology, MESH: Lung Diseases/epidemiology, medicine.medical_specialty, 030106 microbiology, MESH: Pleuropneumonia/drug therapy, 03 medical and health sciences, MESH: Listeriosis/complications, Sepsis, Internal medicine, Pneumonia, Bacterial, medicine, Humans, pneumonia, MESH: Sepsis/drug therapy, MESH: Lung Diseases/drug therapy, [SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology, MESH: Respiratory Tract Infections/drug therapy, Empyema, Pleural, Aged, Retrospective Studies, MESH: Pleuropneumonia/etiology, MESH: Sepsis/microbiology, Lung, MESH: Humans, business.industry, MESH: Respiratory Tract Infections/microbiology, MESH: Adult, MESH: Retrospective Studies, Amoxicillin, medicine.disease, Listeria monocytogenes, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, Empyema, MESH: Male, Pneumonia, MESH: Lung Diseases/etiology, Concomitant, MESH: Listeriosis/microbiology, MESH: Listeria monocytogenes/drug effects, MESH: Pleuropneumonia/epidemiology, [SDV.MP.BAC] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, business, MESH: Female, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract: Listeria monocytogenes (Lm) is a foodborne human pathogen responsible for severe infections, including septicaemia, neurolisteriosis, and maternal-foetal and focal infections. Little is known about Lm-associated respiratory tract or lung infections.We conducted a retrospective study of culture-proven cases of Lm pleural infections and pneumonia reported to the French National Reference Centre for Listeria from January 1993 to August 2016.Thirty-eight consecutive patients with pleural infection (n = 32), pneumonia (n = 5), or both (n = 1) were studied; 71% of these were men. Median age was 72 (range 29-90). Two patients presented with concomitant neurolisteriosis. All patients but one reported at least one immunosuppressive condition (97%), with a median number of 2 (range 0-5), including 29% (8/28) with current exposure to immunosuppressive therapy and 50% (17/34) with ongoing neoplasia; 75% (21/28) reported previous pleural or pulmonary disease. Antibiotic therapy mostly consisted in amoxicillin (72%) associated with aminoglycoside in 32%. Chest-tube drainage was performed in 7/19 patients with empyema (37%); 25% of the patients (7/30) required intensive care management. In-hospital mortality reached 35% and occurred after a median time interval of 4 days (range 1-33 days). Three patients had recurrence of empyema (time interval of 1 week to 4 months after treatment completion). Altogether, only 13/31 patients (42%) diagnosed with Lm respiratory infection experienced an uneventful outcome at 2-year follow-up.Lm is a rare but severe cause of pneumonia and pleural infection in older immunocompromised patients, requiring prompt diagnosis and adequate management and follow-up.
Published: 2018
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16. Prevalence of Listeria spp. and characterization of Listeria monocytogenes isolated from food products in Tetouan, Morocco

Author: Hélène Bracq-Dieye, Marc Lecuit, José Miguel Soriano, Mohammed El Maadoudi, Jamal Abrini, Nadia Skalli Senhaji, Alexandre Leclercq, Ayoub Kounnoun, Alexandra Moura, Nadia Amajoud, Université Abdelmalek Essaâdi (UAE), Centre National de Référence Listeria - Biologie des Infections (CNRL), Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre collaborateur de l'OMS Listeria - Biologie des Infections (CCOMS), Biologie des Infections - Biology of Infection, Universitat de València (UV), Office National de Sécurité Sanitaire des Produits Alimentaires [Maroc] (ONSSA), Service des Maladies infectieuses et tropicales [CHU Necker], Université Paris Descartes - Paris 5 (UPD5)-CHU Necker - Enfants Malades [AP-HP]-Institut des Maladies Génétiques Imagine [Paris], Université Paris Descartes - Paris 5 (UPD5), CHU Necker - Enfants Malades [AP-HP], Centre National de Référence Listeria - National Reference Center Listeria (CNRL), Centre collaborateur de l'OMS Listeria / WHO Collaborating Centre Listeria (CC-OMS / WHO-CC), Institut Pasteur [Paris]-Organisation Mondiale de la Santé / World Health Organization Office (OMS / WHO), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), and Institut Pasteur [Paris] (IP)-Organisation Mondiale de la Santé / World Health Organization Office (OMS / WHO)
Subjects: 0301 basic medicine, medicine.drug_class, Listeria, PCR-Serogrouping, 030106 microbiology, Antibiotics, Prevalence, Biology, medicine.disease_cause, Microbiology, 03 medical and health sciences, Listeria monocytogenes, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, medicine, ComputingMilieux_MISCELLANEOUS, 2. Zero hunger, Gel electrophoresis, business.industry, PFGE, biology.organism_classification, Food safety, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, Morocco, 030104 developmental biology, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, Food products, Multilocus sequence typing, cgMLST, business, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology, Food Science, Biotechnology
Abstract: A total of 1096 food samples were purchased in Tetouan, North-Western of Morocco, to examine the presence of Listeria spp. Eighty (7.3%) of the tested samples were found positive for the presence of Listeria spp., while L. monocytogenes was detected in 16 (1.5%) samples. L. monocytogenes isolates belonged to clonal complexes CC2 (PCR serogroup IVb) and CC199 (PCR serogroup Ila) and to 8 different combined AscI/Apal pulsed-field gel electrophoresis profiles. Core genome multilocus sequence typing (cgMLST) allowed to distinguish 12 different cgMLST types and revealed the presence of 3 clusters of closely-related isolates from different samples, suggesting the existence of common sources of contamination. Isolates showed no resistance to the reference antibiotics used for the treatment of listeriosis. This study underlines the circulation of L. monocytogenes strains of clonal complex CC2 in Morocco and highlights the importance of microbiological surveillance in order to minimize consumers' exposure to this foodborne pathogen. (C) 2017 Elsevier Ltd. All rights reserved.
Published: 2018
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17. MALDI-TOF mass spectrometry-based identification of Listeria species in surveillance: a prospective study

Author: Guillaume Vales, Mylène M. Maury, Pierre Thouvenot, Marc Lecuit, Alexandra Moura, Hélène Bracq-Dieye, Nathalie Tessaud-Rita, Alexandre Leclercq, Centre collaborateur de l'OMS Listeria / WHO Collaborating Centre Listeria (CC-OMS / WHO-CC), Institut Pasteur [Paris] (IP)-Organisation Mondiale de la Santé / World Health Organization Office (OMS / WHO), Biologie des Infections - Biology of Infection, Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Paris Descartes - Paris 5 (UPD5), CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Imagine - Institut des maladies génétiques (IHU) (Imagine - U1163), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité), This work was supported by Institut Pasteur and Public Health France., We thank Dr. Martin Wiedmann (Cornell University, USA) for providing type strains of L. marthii, L. grandensis, L. aquatica, L. cornellensis, L. riparia and L. floridensis, Dr. Leo Meile (Swiss Federal Institute of Technology Zurich, Switzerland) for L. fleischmannii, Dr. C. Bizet (Collection de l'Institut Pasteur, France) for providing type strains of L. weihenstephanensis, L. booriae, L. newyorkensis and the access to the Bruker Daltonics MALDI-TOF MS system. We are also thankful to the P2M platform (Institut Pasteur, Paris, France) for genome sequencing., Institut Pasteur [Paris]-Organisation Mondiale de la Santé / World Health Organization Office (OMS / WHO), Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Paris (UP), and DIAKITE, andrée
Subjects: 0301 basic medicine, Microbiology (medical), MALDI-TOF, MESH: Sequence Analysis, DNA, Listeria, 030106 microbiology, Context (language use), Biology, Bioinformatics, Listeria identification, Microbiology, MESH: Bacterial Typing Techniques, Listeria species, 03 medical and health sciences, MESH: Listeria, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, Public health surveillance, Prospective Studies, MESH: Food Microbiology, Molecular Biology, [SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology, Whole Genome Sequencing, Mass spectrometry, MESH: Public Health Surveillance, Computational Biology, Sequence Analysis, DNA, MALDI-TOF Mass Spectrometry, biology.organism_classification, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, MESH: Prospective Studies, Bacterial Typing Techniques, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, MESH: Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Food, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Food Microbiology, [SDV.MHEP.MI] Life Sciences [q-bio]/Human health and pathology/Infectious diseases, Identification (biology), [SDV.MP.BAC] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, MESH: Whole Genome Sequencing, MESH: Computational Biology
Abstract: International audience; This study aimed to evaluate MALDI-TOF MS for species discrimination of Listeria in the context of routine surveillance. MALDI-TOF MS yielded 100% accuracy for the identification of L. monocytogenes, L. innocua, L. ivanovii, L. fleischmannii, L. grayi, L. seeligeri, L. weihenstephanensis and L. welshimeri, as confirmed by whole genome sequence analyses.
Published: 2017
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18. Spontaneous Loss of Virulence in Natural Populations of Listeria monocytogenes

Author: Mariela Scortti, Alexandra Moura, Lei Han, Guillaume Vales, Alexandre Leclercq, Olivier Disson, Marc Lecuit, Hélène Bracq-Dieye, Mylène M. Maury, Edith Gouin, José A. Vázquez-Boland, Viviane Chenal-Francisque, Centre collaborateur de l'OMS Listeria / WHO Collaborating Centre Listeria (CC-OMS / WHO-CC), Institut Pasteur [Paris] (IP)-Organisation Mondiale de la Santé / World Health Organization Office (OMS / WHO), Centre National de Référence Listeria - National Reference Center Listeria (CNRL), Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), University of Edinburgh, Biologie des Infections - Biology of Infection, Interactions Bactéries-Cellules (UIBC), Institut National de la Recherche Agronomique (INRA)-Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Paris Descartes - Paris 5 (UPD5), CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Imagine - Institut des maladies génétiques (IHU) (Imagine - U1163), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité), Work in M.L.'s laboratory was supported by Institut Pasteur, Inserm, LabEx IBEID, the European Research Council, and Santé Publique France. Work in J.A.V.-B.'s laboratory was supported by the Wellcome Trust (program grant WT074020MA) and partially by core Roslin Institute funding from BBSRC (BB/J004227/1), We acknowledge Anne Morvan, Thomas Cantinelli, Nathalie Tessaud-Rita and Laetitia Bellon for their help in strain collection and isolation at the National Reference Centre for Listeria. We thank Pascale Cossart for providing antibodies against LLO and the Institut Pasteur P2M platform for genome sequencing., ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), DIAKITE, andrée, Integrative Biology of Emerging Infectious Diseases - - IBEID2010 - ANR-10-LABX-0062 - LABX - VALID, Institut Pasteur [Paris]-Organisation Mondiale de la Santé / World Health Organization Office (OMS / WHO), Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut National de la Recherche Agronomique (INRA)-Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Paris (UP), Centre collaborateur de l'OMS Listeria - Biologie des Infections (CCOMS), Centre National de Référence Listeria - Biologie des Infections (CNRL), Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Pasteur [Paris]-Institut National de la Recherche Agronomique (INRA), Institut des Maladies Génétiques Imagine [Paris], and ANR-10-LABX-62-IBEID,IBEID,Laboratoire d'Excellence 'Integrative Biology of Emerging Infectious Diseases'(2010)
Subjects: 0301 basic medicine, Erythrocytes, MESH: Selection, Genetic, Mutant, MESH: Heat-Shock Proteins, MESH: Virulence, medicine.disease_cause, MESH: Listeria monocytogenes, Severity of Illness Index, MESH: Recombinant Proteins, Hemolysin Proteins, Mice, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, Listeriosis, MESH: Animals, Cloning, Molecular, MESH: Phylogeny, Pathogen, MESH: Bacterial Proteins, Heat-Shock Proteins, Phylogeny, Mice, Inbred BALB C, MESH: Gene Expression Regulation, Bacterial, [SDV.MHEP] Life Sciences [q-bio]/Human health and pathology, MESH: Erythrocytes, Listeriolysin O, Biological Evolution, spontaneous mutations, MESH: Amino Acid Substitution, MESH: Hemolysis, Recombinant Proteins, Infectious Diseases, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, MESH: Hemolysin Proteins, [SDV.MHEP.MI] Life Sciences [q-bio]/Human health and pathology/Infectious diseases, MESH: Peptide Termination Factors, Peptide Termination Factors, Transposable element, MESH: Mutation, Bacterial Toxins, 030106 microbiology, Immunology, MESH: Mice, Inbred BALB C, Virulence, Molecular Genomics, MESH: Biological Evolution, Biology, Microbiology, Frameshift mutation, 03 medical and health sciences, Bacterial Proteins, Listeria monocytogenes, MESH: Severity of Illness Index, medicine, genomics, Animals, Humans, MESH: Cloning, Molecular, Selection, Genetic, [SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology, Gene, MESH: Mice, MESH: Humans, Gene Expression Regulation, Bacterial, biochemical phenomena, metabolism, and nutrition, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, virulence, Amino Acid Substitution, MESH: Bacterial Toxins, MESH: Listeriosis, Mutation, Parasitology, [SDV.MP.BAC] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, hemolysis, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract: The pathogenesis of Listeria monocytogenes depends on the ability of this bacterium to escape from the phagosome of the host cells via the action of the pore-forming toxin listeriolysin O (LLO). Expression of the LLO-encoding gene ( hly ) requires the transcriptional activator PrfA, and both hly and prfA genes are essential for L. monocytogenes virulence. Here, we used the hemolytic activity of LLO as a phenotypic marker to screen for spontaneous virulence-attenuating mutations in L. monocytogenes . Sixty nonhemolytic isolates were identified among a collection of 57,820 confirmed L. monocytogenes strains isolated from a variety of sources (0.1%). In most cases (56/60; 93.3%), the nonhemolytic phenotype resulted from nonsense, missense, or frameshift mutations in prfA . Five strains carried hly mutations leading to a single amino acid substitution (G299V) or a premature stop codon causing strong virulence attenuation in mice. In one strain, both hly and gshF (encoding a glutathione synthase required for full PrfA activity) were missing due to genomic rearrangements likely caused by a transposable element. The PrfA/LLO loss-of-function (PrfA − /LLO − ) mutants belonged to phylogenetically diverse clades of L. monocytogenes , and most were identified among nonclinical strains (57/60). Consistent with the rare occurrence of loss-of-virulence mutations, we show that prfA and hly are under purifying selection. Although occurring at a low frequency, PrfA − /LLO − mutational events in L. monocytogenes lead to niche restriction and open an evolutionary path for obligate saprophytism in this facultative intracellular pathogen.
Published: 2017
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19. Listeria monocytogenes isolation from urine: a series of 15 cases and review

Author: V. Perronne, Alexandre Leclercq, Alexandra Moura, François Danion, B. Tanguy, M. Dary, Mylène M. Maury, Caroline Charlier, Marc Lecuit, Sophie Leotard, Pierre Thouvenot, Hélène Bracq-Dieye, Centre National de Référence Listeria - National Reference Center Listeria (CNRL), Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre collaborateur de l'OMS Listeria / WHO Collaborating Centre Listeria (CC-OMS / WHO-CC), Institut Pasteur [Paris]-Organisation Mondiale de la Santé / World Health Organization Office (OMS / WHO), Biologie des Infections - Biology of Infection, Université Paris Descartes - Paris 5 (UPD5), CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Imagine - Institut des maladies génétiques (IHU) (Imagine - U1163), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Paris (UP), Centre Hospitalier François Quesnay, Centre hospitalier de Grasse, Centre hospitalier universitaire de Nantes (CHU Nantes), Hôpital de Guingamp, Financial support was received from the Institut Pasteur, Inserm, Santé Publique France., Doctors Bachar Al Jalaby (Centre Hospitalier (CH) Saint Lo, France), Philippe Aucher (CH Saint Jean D'Angely, France), Séverine Chanier (CH Riom, France), Manuel Etienne (CHU Rouen, France), Alix Greder Bellan (CH Versailles, France), Mohamed Guerzou (CH Alès, France), Beatrice Lesimple (CH Cholet, France), Alban Michaud Herbst (CH La Rochelle, France), Loic Mignot (CH Avranches-Granville, France), Ana Mendes Moreira (CH La Rochelle, France), Herve Paris (Clinique Pole Santé République, Clermont-Ferrand, France), Centre National de Référence Listeria - Biologie des Infections (CNRL), Centre collaborateur de l'OMS Listeria - Biologie des Infections (CCOMS), Institut des Maladies Génétiques Imagine [Paris], hôpital François Quesnay, Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Pasteur [Paris] (IP)-Organisation Mondiale de la Santé / World Health Organization Office (OMS / WHO), and Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité)
Subjects: 0301 basic medicine, Microbiology (medical), business.industry, [SDV]Life Sciences [q-bio], 030106 microbiology, General Medicine, Urine, Isolation (microbiology), medicine.disease_cause, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, 3. Good health, Microbiology, 03 medical and health sciences, 030104 developmental biology, Infectious Diseases, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, Listeria monocytogenes, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, medicine, ComputingMethodologies_DOCUMENTANDTEXTPROCESSING, business, ComputingMilieux_MISCELLANEOUS, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract: International audience; Letter to the Editor
Published: 2017
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20. Whole genome-based population biology and epidemiological surveillance of Listeria monocytogenes

Author: Viviane Chenal-Francisque, Marie Touchon, Thomas Cantinelli, Hannes Pouseele, Jonas T. Björkman, Marc Lecuit, Celine Nadon, Aleisha Reimer, Mylène M. Maury, Heather A. Carleton, Lee S. Katz, Zuzana Kucerova, Peter Gerner-Smidt, Alexandre Leclercq, Mathieu Tourdjman, Eduardo P. C. Rocha, Alexandra Moura, Alexis Criscuolo, Kathie Grant, Cheryl L. Tarr, Bruno Pot, Hélène Bracq-Dieye, Vincent Enouf, Elise Larsonneur, Eva Møller Nielsen, Timothy J. Dallman, Sylvain Brisse, Steven Stroika, Matthew G. Walker, Louis Jones, Lassailly-Bondaz, Anne, Integrative Biology of Emerging Infectious Diseases - - IBEID2010 - ANR-10-LABX-0062 - LABX - VALID, Microbial invasion and dissemination within the host, mechanisms and effects - INVADIS - 2016-11-01 - 2021-10-31 - 681829 - VALID, Génomique évolutive des Microbes / Microbial Evolutionary Genomics, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Centre National de Référence Listeria - National Reference Center Listeria (CNRL), Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Hub Bioinformatique et Biostatistique - Bioinformatics and Biostatistics HUB, Applied-Maths, Centers for Disease Control and Prevention [Atlanta] (CDC), Centers for Disease Control and Prevention, Statens Serum Institut [Copenhagen], Public Health England [London], Public Health Agency of Canada, Pasteur International Bioresources network (PIBNet), Institut Pasteur [Paris] (IP), Institut Français de Bioinformatique - UMS CNRS 3601 (IFB-CORE), Institut National de la Recherche Agronomique (INRA)-Institut National de Recherche en Informatique et en Automatique (Inria)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut de Veille Sanitaire (INVS), Centre collaborateur de l'OMS Listeria / WHO Collaborating Centre Listeria (CC-OMS / WHO-CC), Institut Pasteur [Paris] (IP)-Organisation Mondiale de la Santé / World Health Organization Office (OMS / WHO), Biologie des Infections - Biology of Infection, Université Paris Descartes - Paris 5 (UPD5), CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), This work was supported by Institut Pasteur,INSERM, Public Health France, French government’s Investissement d’Avenir programLaboratoire d’Excellence 'Integrative Biology of Emerging Infectious Diseases' (grantANR-10-LABX-62-IBEID), European Research Council, Swiss National Fund for Researchand the Advanced Molecular Detection (AMD) initiative at CDC, ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), European Project: 681829,ERC-CoG-2015,INVADIS(2016), Department of Bio-engineering Sciences, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Pasteur [Paris], Institut Pasteur [Paris]-Organisation Mondiale de la Santé / World Health Organization Office (OMS / WHO), Centre National de Référence Listeria - Biologie des Infections (CNRL), Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur [Paris], Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de la Recherche Agronomique (INRA), Centre collaborateur de l'OMS Listeria - Biologie des Infections (CCOMS), and ANR-10-LABX-62-IBEID,IBEID,Laboratoire d'Excellence 'Integrative Biology of Emerging Infectious Diseases'(2010)
Subjects: 0301 basic medicine, Microbiology (medical), Genotyping Techniques, Lineage (evolution), 030106 microbiology, Immunology, Virulence, Population biology, Biology, Global Health, Applied Microbiology and Biotechnology, Microbiology, Genome, Article, 03 medical and health sciences, Genetics, Humans, Listeriosis, Typing, Genotyping, epidemiological monitoring, Molecular Epidemiology, Molecular Epidemiology/methods, Strain (biology), Genetic Variation, Genotyping Techniques/methods, Outbreak, Cell Biology, Listeria monocytogenes, Listeriosis/epidemiology, 3. Good health, Phylogeography, Listeria monocytogenes/classification, 030104 developmental biology, [SDV.SPEE] Life Sciences [q-bio]/Santé publique et épidémiologie, [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie, Genome, Bacterial
Abstract: International audience; Listeria monocytogenes (Lm) is a major human foodborne pathogen. Numerous Lm outbreaks have been reported worldwide and associated with a high case fatality rate, reinforcing the need for strongly coordinated surveillance and outbreak control. We developed a universally applicable genome-wide strain genotyping approach and investigated the population diversity of Lm using 1,696 isolates from diverse sources and geographical locations. We define, with unprecedented precision, the population structure of Lm, demonstrate the occurrence of international circulation of strains and reveal the extent of heterogeneity in virulence and stress resistance genomic features among clinical and food isolates. Using historical isolates, we show that the evolutionary rate of Lm from lineage I and lineage II is low (∼2.5 × 10-7 substitutions per site per year, as inferred from the core genome) and that major sublineages (corresponding to so-called 'epidemic clones') are estimated to be at least 50-150 years old. This work demonstrates the urgent need to monitor Lm strains at the global level and provides the unified approach needed for global harmonization of Lm genome-based typing and population biology.
Published: 2016
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21. Listérioses pleuropulmonaires : à propos de 38 cas

Author: M. Morgand, Marc Lecuit, Hélène Bracq-Dieye, Guillaume Vales, Alexandre Leclercq, Caroline Charlier, Mylène M. Maury, and Pierre Thouvenot
Subjects: Infectious Diseases
Abstract: Introduction Listeria monocytogenes (Lm) est une bacterie Gram positif de transmission alimentaire, responsable d’infections severes chez les patients immunodeprimes (septicemies, infections du systeme nerveux central), et d’infections materno-neonatales. Des infections respiratoires, pleurales et/ou pulmonaires a Lm ont ete rapportees, sans jamais etre precisement decrites. Materiels et methodes La surveillance des infections a Lm en France repose sur la declaration obligatoire des cas a l’Agence nationale de sante publique et sur l’envoi volontaire des souches au Centre national de reference des Listeria. Nous avons mene une etude retrospective des cas declares de janvier 1993 a juillet 2015 avec identification de Lm dans des prelevements respiratoires, pleuraux ou dans des hemocultures au cours de pneumonies sans autre etiologie identifiee (n = 3). Les donnees cliniques ont ete analysees et les isolats genotypes. Resultats Les donnees de 38 patients ont ete etudiees : 28 pleuresies, 5 pleuropneumopathies, 4 pneumonies et une bronchite. Vingt-huit patients (68 %) etaient des hommes. L’âge median etait de 74 ans [29–90]. Dix patients (27 %) avaient plus de 80 ans. Trente-sept patients (97 %) avaient au moins 1 comorbidite immunosuppressive associee (0 a 5, mediane de 2). La principale etant une neoplasie, presente chez 20 patients (53 %). Les autres etaient : cirrhose (n = 8), ethylisme sans cirrhose (n = 8), diabete (n = 5), maladie auto-immune (n = 3) et insuffisance renale terminale (n = 1). Trois malades presentaient une neurolisteriose et/ou une infection du liquide d’ascite avec une pleuresie. Dix patients (27 %) avaient une bacteriemie associee. Cinq malades (13 %) n’avaient pas de fievre. Parmi les 33 cas de pleuresie, 15 (45 %) avaient une pathologie pleurale sous-jacente (transudative, neoplasique, tuberculeuse, pleuresie recurrente idiopathique) guerie ou actuelle. Le liquide pleural etait exsudatif dans 72 % des cas (liquide purulent 61 %), hemorragique (17 %) ou transudatif (11 %). Vingt-sept patients (72 %) ont recu de l’amoxicilline et 12 (32 %) une association amoxicilline-aminoside. La duree mediane de traitement etait de 14 jours (extremes [0–42]). Elle etait de 21 jours [10–42] parmi les survivants. La duree mediane de suivi etait de 9 mois. Quinze patients sont decedes (39 %). Le delai median entre le diagnostic et le deces etait de 4 jours ([1–23]). Conclusion Les infections pleuropulmonaires a Lm sont une entite reelle. Elles surviennent chez des patients fragiles, âges et immunodeprimes, dont plus de 50 % ont une neoplasie sous-jacente. Le pronostic de ces formes est tres defavorable, les classant, avec les septicemies, parmi les formes les plus graves de listerioses.
Published: 2017
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22. Listeria monocytogenes-associated biliary tract infections: a study of 12 consecutive cases and review

Author: Céline Bossard, Benoît Cazenave, Laetitia Travier, Caroline Charlier, Juliette Podevin, Daher Assomany, Lydie Guilbert, Valérie Cales, Hélène Bracq-Dieye, Marc Lecuit, Françoise Carpentier, Cindy Fevre, and Alexandre Leclercq
Subjects: Male, Cholangitis, Biliary Cyst, Bacteremia, Comorbidity, medicine.disease_cause, Gastroenterology, Cholecystitis, Medication Errors, Listeriosis, Aged, 80 and over, 0303 health sciences, biology, Cysts, General Medicine, Middle Aged, 3. Good health, Anti-Bacterial Agents, medicine.anatomical_structure, Biliary tract, Population Surveillance, Female, France, Immunosuppressive Agents, Adult, medicine.medical_specialty, Fever, Biliary Tract Diseases, Gallbladder disease, Observational Study, Gallbladder Diseases, Penicillins, Article, 03 medical and health sciences, Necrosis, Listeria monocytogenes, Internal medicine, Drug Resistance, Bacterial, medicine, Humans, Cholecystectomy, 030304 developmental biology, Aged, Retrospective Studies, 030306 microbiology, business.industry, Gallbladder, Cholecystolithiasis, biology.organism_classification, medicine.disease, Surgery, Abdominal Pain, Chronic infection, Listeria, business
Abstract: At present, little is known regarding Listeria monocytogenes-associated biliary tract infection, a rare form of listeriosis. In this article, we will study 12 culture-proven cases reported to the French National Reference Center for Listeria from 1996 to 2013 and review the 8 previously published cases. Twenty cases were studied: 17 cholecystitis, 2 cholangitis, and 1 biliary cyst infection. Half were men with a median age of 69 years (32–85). Comorbidities were present in 80%, including cirrhosis, rheumatoid arthritis, and diabetes. Five patients received immunosuppressive therapy, including corticosteroids and anti-tumor necrosis factor biotherapies. Half were afebrile. Blood cultures were positive in 60% (3/5). Gallbladder histological lesions were analyzed in 3 patients and evidenced acute, chronic, or necrotic exacerbation of chronic infection. Genoserogroup of the 12 available strains were IVb (n = 6), IIb (n = 5), and IIa (n = 1). Their survival in the bile was not enhanced when compared with isolates from other listeriosis cases. Adverse outcome was reported in 33% (5/15): 3 deaths, 1 recurrence; 75% of the patients with adverse outcome received inadequate antimicrobial therapy (P = 0.033). Biliary tract listeriosis is a severe infection associated with high mortality in patients not treated with appropriate therapy. This study provides medical relevance to in vitro and animal studies that had shown Listeria monocytogenes ability to survive in bile and induce overt biliary infections.
Published: 2014

23. Optimized Multilocus variable-number tandem-repeat analysis assay and its complementarity with pulsed-field gel electrophoresis and multilocus sequence typing for Listeria monocytogenes clone identification and surveillance

Author: Viviane Chenal-Francisque, Laure Diancourt, Hélène Bracq-Dieye, Coralie Tran-Hykes, Virginie Passet, Marc Lecuit, Alexandre Leclercq, Sylvain Brisse, Christine Pourcel, Thomas Cantinelli, Centre National de Référence Listeria - National Reference Center Listeria (CNRL), Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre collaborateur de l'OMS Listeria / WHO Collaborating Centre Listeria (CC-OMS / WHO-CC), Institut Pasteur [Paris]-Organisation Mondiale de la Santé / World Health Organization Office (OMS / WHO), Biologie des Infections - Biology of Infection, Génotypage des Pathogènes et Santé Publique (Plate-forme) (PF8), Institut Pasteur [Paris], Institut de génétique et microbiologie [Orsay] (IGM), Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS), Université Paris Descartes - Paris 5 (UPD5), CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Service des Maladies infectieuses et tropicales [CHU Necker], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Génomique évolutive des Microbes / Microbial Evolutionary Genomics, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), DIAKITE, andrée, Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Pasteur [Paris] (IP)-Organisation Mondiale de la Santé / World Health Organization Office (OMS / WHO), Institut Pasteur [Paris] (IP), and Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS)
Subjects: Microbiology (medical), DNA, Bacterial, Genotype, Epidemiology, [SDV]Life Sciences [q-bio], Minisatellite Repeats, Multiple Loci VNTR Analysis, Biology, 03 medical and health sciences, Tandem repeat, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, Pulsed-field gel electrophoresis, Animals, Cluster Analysis, Humans, Mass Screening, Listeriosis, [SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology, Mass screening, 030304 developmental biology, 2. Zero hunger, Genetics, 0303 health sciences, Molecular Epidemiology, [SDV.MHEP] Life Sciences [q-bio]/Human health and pathology, Molecular epidemiology, 030306 microbiology, Reproducibility of Results, bacterial infections and mycoses, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, Listeria monocytogenes, Subtyping, 3. Good health, Electrophoresis, Gel, Pulsed-Field, [SDV] Life Sciences [q-bio], Variable number tandem repeat, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, [SDV.MHEP.MI] Life Sciences [q-bio]/Human health and pathology/Infectious diseases, Multilocus sequence typing, [SDV.MP.BAC] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology, Multilocus Sequence Typing
Abstract: International audience; Populations of the food-borne pathogen Listeria monocytogenes are genetically structured into a small number of major clonal groups, some of which have been implicated in multiple outbreaks. The goal of this study was to develop and evaluate an optimized multilocus variable number of tandem repeat (VNTR) analysis (MLVA) subtyping scheme for strain discrimination and clonal group identification. We evaluated 18 VNTR loci and combined the 11 best ones into two multiplexed PCR assays (MLVA-11). A collection of 255 isolates representing the diversity of clonal groups within phylogenetic lineages I and II, including representatives of epidemic clones, were analyzed by MLVA-11, multilocus sequence typing (MLST), and pulsed-field gel electropho-resis (PFGE). MLVA-11 had less discriminatory power than PFGE, except for some clones, and was unable to distinguish some epidemiologically unrelated isolates. Yet it distinguished all major MLST clones and therefore constitutes a rapid method to identify epidemiologically relevant clonal groups. Given its high reproducibility and high throughput, MLVA represents a very attractive first-line screening method to alleviate the PFGE workload in outbreak investigations and listeriosis surveillance. L isteriosis is a food-borne infection caused by the bacterium Listeria monocytogenes. Invasive forms of human listeriosis include septicemia, meningitis, and maternal-fetal infections (1). Listeriosis is associated with high hospitalization and fatality rates (almost 100% and 25 to 30%, respectively). Populations at risk include pregnant women, immunocompromised individuals, and the elderly. L. monocytogenes is widely present in the environment, including soil, water, vegetation, and silage, as well as in animals and animal-derived food, and can contaminate food in processing plants and retail establishments. L. monocytogenes is recognized as a public health issue and a serious challenge for the food industry, and this has led to the establishment of national surveillance systems in several countries. L. monocytogenes also stands out as a model system in the fields of microbiology, cell biology, and im-munology and for the study of host-pathogen interactions (2-5). L. monocytogenes strain characterization on the basis of sero-typing and molecular typing methods is used for surveillance, epidemiological tracking, and outbreak investigation purposes (6, 7). Genetic variants of L. monocytogenes have diversified into four major phylogenetic lineages, with lineages 1 and 2 each containing multiple clonal groups of public health importance (8-14). As these groups appear to differ in virulence and epidemic potential (6, 15), it will be interesting to better define their epidemiological, clinical, and microbiological specificities. For this purpose, tools for the easy identification of clonal groups are needed to recognize such groups and determine their presence in a large variety of sources. Several typing methods are currently available for L. monocytogenes strains. Conventional serotyping (16) and its molecular proxy PCR serogrouping (17) discriminate major categories of strains that correlate strongly (albeit not totally) with lin-eages and clones (11, 12, 14), but these methods do not have the necessary discriminatory power in the context of outbreak investigations. Pulsed-field gel electrophoresis (PFGE) is established as the gold standard for L. monocytogenes strain subtyping and is widely used for listeriosis surveillance and outbreak investigations (18). Yet, PFGE presents several practical disadvantages, as it is time-consuming and requires stringent standardization for inter-laboratory data comparison. Multilocus sequence typing (MLST) is a well-established reference method for global epidemiology and population biology (19, 20), as it renders interlaboratory genotype comparisons easy and unambiguous and as sequence data can be used to infer useful population genetic information such as amounts of genetic diversity, recombination rates, and strain phy-logeny. MLST also provides backward compatibility with genome sequencing (21). However, MLST is neither rapid nor cheap and has limited discriminatory power within L. monocytogenes (12, 22). Given the current limitations of available methods for L. monocytogenes strain typing, a potentially useful complementary approach is multilocus variable number of tandem repeats
Published: 2013
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24. Listeria spp. Isolated from Tonsils of Wild Deer and Boars: Genomic Characterization

Author: Carla Palacios-Gorba, Juan J. Quereda, Marc Lecuit, Jesús Gomis, María L. Mocé, Ángel Gómez-Martín, Alexandre Leclercq, E. Jiménez-Trigos, Alexandra Moura, Universidad Cardenal Herrera-CEU (CEU-UCH), Centre National de Référence Listeria - National Reference Center Listeria (CNRL), Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre collaborateur de l'OMS Listeria / WHO Collaborating Centre Listeria (CC-OMS / WHO-CC), Institut Pasteur [Paris] (IP)-Organisation Mondiale de la Santé / World Health Organization Office (OMS / WHO), Service des Maladies infectieuses et tropicales [CHU Necker], CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Imagine - Institut des maladies génétiques (IHU) (Imagine - U1163), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité), This work was supported by Generalitat Valenciana (project reference GV/2018/A/183) and by the Spanish Ministry of Science and Innovation (project reference PID2019-110764RA-I00) (J.J.Q.), Institut Pasteur, Inserm, and Santé Publique France. J. J. Quereda is supported by a 'Ramón y Cajal' contract of the Spanish Ministry of Science, Innovation and Universities (RYC-2018-024985-I). C. Palacios-Gorba is supported by a predoctoral contract from the Universidad Cardenal Herrera-CEU., We thank Hélène Bracq-Dieye, Nathalie Tessaud-Rita, Pierre Thouvenot, and Guillaume Vales (Institut Pasteur, Paris, France) for DNA extraction and the P2M platform (Institut Pasteur, Paris, France) for genome sequencing., DIAKITE, andrée, Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Pasteur [Paris]-Organisation Mondiale de la Santé / World Health Organization Office (OMS / WHO), and Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Paris (UP)
Subjects: [SDV.BA] Life Sciences [q-bio]/Animal biology, [SDV]Life Sciences [q-bio], medicine.disease_cause, MESH: Genome, Bacterial, Applied Microbiology and Biotechnology, MESH: Listeria, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, MESH: Animals, MESH: Phylogeny, Pathogen, 2. Zero hunger, 0303 health sciences, education.field_of_study, [SDV.MHEP] Life Sciences [q-bio]/Human health and pathology, Ecology, Public and Environmental Health Microbiology, [SDV.BA]Life Sciences [q-bio]/Animal biology, MESH: Feces, 3. Good health, wild animal, [SDV] Life Sciences [q-bio], MESH: Multilocus Sequence Typing, MESH: Palatine Tonsil, [SDV.MHEP.MI] Life Sciences [q-bio]/Human health and pathology/Infectious diseases, Listeria ivanovii, MESH: Whole Genome Sequencing, Biotechnology, Population, MESH: Deer, Biology, Microbiology, 03 medical and health sciences, Listeria monocytogenes, medicine, education, Feces, 030304 developmental biology, 030306 microbiology, biology.organism_classification, Pathogenicity island, MESH: Sus scrofa, virulence, MESH: Listeriosis, tonsil, Listeria, Multilocus sequence typing, cgMLST, WGS, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology, Food Science
Abstract: Listeria monocytogenes is a major human and animal foodborne pathogen. However, data from environmental reservoirs remain scarce. Here, we used whole-genome sequencing to characterize Listeria species isolates recovered over 1 year from wild animals in their natural habitats in Spain. Three different Listeria spp. (L. monocytogenes [n = 19], Listeria ivanovii subsp. londoniensis [n = 4], and Listeria innocua [n = 3]) were detected in 23 animal tonsils (9 deer, 14 wild boars) and 2 feeding troughs. No Listeria species was detected in feces. L. monocytogenes was detected in tonsils of 44.4% (8 out of 18) of deer and 40.7% (11 out of 27) of wild boars. L. monocytogenes isolates belonged to 3 different core genome multilocus sequence typing (cgMLST) types (CTs) of 3 distinct sublineages (SL1, SL387, and SL155) from lineages I and II. While cgMLST type L1-SL1-ST1-CT5279 (IVb; clonal complex 1 [CC1]) occurred only in one animal, types L1-SL387-ST388-CT5239 (IVb; CC388) and L2-SL155-ST155-CT1170 (IIa; CC155) were retrieved from multiple animals. In addition, L1-SL387-ST388-CT5239 (IVb; CC388) isolates were collected 1 year apart, revealing their long-term occurrence within the animal population and/or environmental reservoir. The presence of identical L. monocytogenes strains in deer and wild boars suggests contamination from a common food or environmental source, although interhost transmission cannot be excluded. Pathogenicity islands LIPI-1, LIPI-3, and LIPI-4 were present in 100%, 5%, and 79% of the L. monocytogenes isolates, respectively, and all L. monocytogenes lineage II isolates (n = 3) carried SSI-1 stress islands. This study highlights the need for monitoring L. monocytogenes environmental contamination and the importance of tonsils as a possible L. monocytogenes intrahost reservoir. IMPORTANCEListeria monocytogenes is a foodborne bacterial pathogen responsible for listeriosis. Whole-genome sequencing has been extensively used in public health and food industries to characterize circulating Listeria isolates, but genomic data on isolates occurring in natural environments and wild animals are still scarce. Here, we show that wild animals carry pathogenic Listeria and that the same genotypes can be found at different time points in different host species. This work highlights the need of Listeria species monitoring of environmental contamination and the importance of tonsils as a possible L. monocytogenes intrahost reservoir.
Published: 2021
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25. Listeria monocytogenes faecal carriage is common and depends on the gut microbiota

Author: Lukas Hafner, Marc Garcia-Garcera, Christophe Burucoa, Marc Lecuit, Sophie H A Nusser, Maxime Pichon, Alexandra Moura, Frasca, Guillaume, Integrative Biology of Emerging Infectious Diseases - - IBEID2010 - ANR-10-LABX-0062 - LABX - VALID, Institut Pasteur International Docotal Program - PASTEURDOC - - H20202015-10-01 - 2020-10-01 - 665807 - VALID, Biologie des Infections - Biology of Infection, Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité), Centre hospitalier universitaire de Poitiers (CHU Poitiers), Pharmacologie des anti-infectieux (PHAR), Université de Poitiers-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre National de Référence Listeria - National Reference Center Listeria (CNRL), Centre collaborateur de l'OMS Listeria / WHO Collaborating Centre Listeria (CC-OMS / WHO-CC), Institut Pasteur [Paris] (IP)-Organisation Mondiale de la Santé / World Health Organization Office (OMS / WHO)-Université Paris Cité (UPCité), Université de Lausanne = University of Lausanne (UNIL), Service des Maladies infectieuses et tropicales [CHU Necker], CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Imagine - Institut des maladies génétiques (IHU) (Imagine - U1163), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité), L.H. is supported by the Pasteur-Paris University (PPU) International Ph.D. Programme, funded by the European Union’s Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement No 665807, the ‘Ecole Doctorale FIRE-Programme Bettencourt’ of the CRI Paris and the Fondation Pasteur Suisse. M.L. laboratory is funded by Institut Pasteur, Inserm, the European Research Council and Laboratoire d’Excellence Integrative Biology of Emerging Infectious Diseases. M.L. is a member of Institut Universitaire de France., We thank Hélène Bracq-Dieye for technical help, Georges Michel Haustant, Cédric Fund, Elodie Turc, Laure Lemée, Biomics Platform, C2RT, Institut Pasteur, Paris, France, supported by France Génomique (ANR-10-INBS-09-09) and IBISA for 16S sequencing, Auguste Fourneau and Amandine Brunet for PCR assays and Sandrine Isaac, Henrik Salje and Olivier Disson for critical reading., ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), European Project: 665807,H2020,H2020-MSCA-COFUND-2014,PASTEURDOC(2015), Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire Inflammation, Tissus épithéliaux et Cytokines (LITEC), Université de Poitiers, Institut Pasteur [Paris]-Organisation Mondiale de la Santé / World Health Organization Office (OMS / WHO), Université de Lausanne (UNIL), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Paris (UP), and Centre National de Référence Listeria - National Reference Center Listeria (CNR)
Subjects: DNA, Bacterial, Male, Science, [SDV]Life Sciences [q-bio], General Physics and Astronomy, Virulence, Datasets as Topic, Gut flora, medicine.disease_cause, General Biochemistry, Genetics and Molecular Biology, Article, Microbiology, 03 medical and health sciences, Feces, Mice, fluids and secretions, Listeria monocytogenes, Abundance (ecology), RNA, Ribosomal, 16S, medicine, Animals, Humans, Phylogeny, 030304 developmental biology, 0303 health sciences, Multidisciplinary, biology, 030306 microbiology, Inoculation, General Chemistry, Bacterial pathogenesis, biology.organism_classification, 16S ribosomal RNA, Gastrointestinal Microbiome, [SDV] Life Sciences [q-bio], Disease Models, Animal, Metagenomics, Carrier State, Listeria
Abstract: Listeria genus comprises two pathogenic species, L. monocytogenes (Lm) and L. ivanovii, and non-pathogenic species. All can thrive as saprophytes, whereas only pathogenic species cause systemic infections. Identifying Listeria species’ respective biotopes is critical to understand the ecological contribution of Listeria virulence. In order to investigate the prevalence and abundance of Listeria species in various sources, we retrieved and analyzed 16S rRNA datasets from MG-RAST metagenomic database. 26% of datasets contain Listeria sensu stricto sequences, and Lm is the most prevalent species, most abundant in soil and host-associated environments, including 5% of human stools. Lm is also detected in 10% of human stool samples from an independent cohort of 900 healthy asymptomatic donors. A specific microbiota signature is associated with Lm faecal carriage, both in humans and experimentally inoculated mice, in which it precedes Lm faecal carriage. These results indicate that Lm faecal carriage is common and depends on the gut microbiota, and suggest that Lm faecal carriage is a crucial yet overlooked consequence of its virulence., Here, the authors show that Listeria monocytogenes faecal carriage is common in healthy asymptomatic individuals and, together with mouse studies, they associate carriage with a specific microbiome signature.
Published: 2021
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25 results on '"Hélène Bracq-Dieye"'

1. Genome-Based Characterization of Listeria monocytogenes, Costa Rica

2. Phenotypic and genotypic antimicrobial resistance of Listeria monocytogenes: an observational study in FranceResearch in context

3. Hypervirulent Listeria monocytogenes clones’ adaption to mammalian gut accounts for their association with dairy products

4. Real-Time Whole-Genome Sequencing for Surveillance of Listeria monocytogenes, France

5. Author Correction: Hypervirulent Listeria monocytogenes clones’ adaptation to mammalian gut accounts for their association with dairy products

6. Phenotypic and Genotypic Antimicrobial Resistance of Listeria Monocytogenes in France

7. Genomic characterization of Listeria spp. isolated from tonsils, udder and feces of domestic dairy ruminants in Spain

8. Listeria valentina sp. nov., isolated from a water trough and the faeces of healthy sheep

9. Listeria-associated lymphadenitis: a series of 11 consecutive cases and review of the literature

10. Ruminant-associated Listeria monocytogenes isolates belong preferentially to dairy-related hypervirulent clones: a longitudinal study in 19 farms

11. Neonatal Listeriosis Presentation and Outcome: A Prospective Study of 189 Cases

12. Cutaneous listeriosis, a case series of 16 consecutive patients over 25 years

13. Author Correction: Hypervirulent Listeria monocytogenes clones’ adaptation to mammalian gut accounts for their association with dairy products

14. Listeria monocytogenes-associated endovascular infections: A study of 71 consecutive cases

15. Listeria monocytogenes-associated respiratory infections: a study of 38 consecutive cases

16. Prevalence of Listeria spp. and characterization of Listeria monocytogenes isolated from food products in Tetouan, Morocco

17. MALDI-TOF mass spectrometry-based identification of Listeria species in surveillance: a prospective study

18. Spontaneous Loss of Virulence in Natural Populations of Listeria monocytogenes

19. Listeria monocytogenes isolation from urine: a series of 15 cases and review

20. Whole genome-based population biology and epidemiological surveillance of Listeria monocytogenes

21. Listérioses pleuropulmonaires : à propos de 38 cas

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23. Optimized Multilocus variable-number tandem-repeat analysis assay and its complementarity with pulsed-field gel electrophoresis and multilocus sequence typing for Listeria monocytogenes clone identification and surveillance

24. Listeria spp. Isolated from Tonsils of Wild Deer and Boars: Genomic Characterization

25. Listeria monocytogenes faecal carriage is common and depends on the gut microbiota

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25 results on '"Hélène Bracq-Dieye"'

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