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DESCRIPTION OF NONOMURAEA MESOPHILA SP. NOV. Nonomuraea mesophila (me.so ′ phi.la Gr. n. mesos middle; Gr. fem. adj. philos loving; N.L. fem. adj. mesophila middle temperature loving). Aerobic, Gram-stain-positive, non-motile actinobacterium that forms extensively branched substrate and aerial mycelia. Aerial hyphae differentiate into spore chains with smooth surfaces. Good growth occurs on ISP 2, ISP 4, modified Bennett’s and tryptic soy agar and moderate growth on Czapek’s, nutrient and ISP 5–7 agar. A range of substrate mycelial pigments are formed including cream, light brown, dark brown, reddish brown, reddish black or claret red ones. Melanoid pigments are not produced on the ISP 6 agar. Red coloured diffusible pigments are observed on modified Bennett’s agar. Grows from 20–37 °C (optimum, 28 °C), from pH 6.0–8.0 (optimum, pH 7.0) and in the presence of up to 5 % (w/v) NaCl. Hydrolyses aesculin, reduces nitrate, but does not produce H 2 S, does not hydrolyse allantoin, arbutin or urea. Degrades hypoxanthine, starch and Tweens (20, 40 and 80), but not adenine, casein, chitin, gelatin, guanine, xanthine or xylan. Adonitol, D-arabinose, D-fructose, raffinose, cellobiose, D-galactose, melezitose, melibiose, dextran, D-glucose, inulin, lactose, D-maltose, D-mannitol, sucrose and D-xylose are used as sole carbon and energy sources but not D-sorbitol, D-mannose, dextrin, L-sorbose, L-arabinose, L-rhamnose, L-glutamine, myo-inositol, sodium succinate or xylitol. L-Alanine, L-arginine, L-asparagine, L-cysteine, glycine, L-hydroxyproline, α-isoleucine, L-methionine, L-phenylalanine, L-proline, L-serine, L-tyrosine and L-valine are utilized as sole nitrogen sources, but not L-histidine. The predominant menaquinones are MK-9(H 4) and MK-9(H 6). The polar lipid profile include diphosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, hydroxy-phosphatidylethanolamine, dihydroxy-phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, two unidentified aminophospholipids, an unidentified glycophospholipid, four unidentified phospholipids and an unidentified lipid. Whole-cell hydrolysates include meso -A 2 pm, glucose, mannose, madurose and ribose. The major fatty acids are C 16:0, iso-C 16:0 and C 17:0 10-methyl. The DNA G+C content of strain 6K102 T is 70.8 mol% and its genome size 10.14 Mbp. The type strain, 6K102 T (=CGMCC 4.7541 T = JCM 32916 T), was isolated from desert soil sample collected from the Karakum Desert, Turkmenistan. The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain 6K102 T is MG770753 and draħ genome sequence accession number SMLD00000000., Published as part of Saygin, Hayrettin, Nouioui, Imen, Ay, Hilal, Guven, Kiymet, Cetin, Demet, Klenk, Hans-Peter, Goodfellow, Michael & Sahin, Nevzat, 2020, Polyphasic classification of Nonomuraea strains isolated from the Karakum Desert and description of Nonomuraea deserti sp. nov., Nonomuraea diastatica sp. nov., Nonomuraea longispora sp. nov. and Nonomuraea mesophila sp. nov., pp. 636-647 in International Journal of Systematic and Evolutionary Microbiology 70 (1) on page 645, DOI: 10.1099/ijsem.0.003808, http://zenodo.org/record/3745026

DESCRIPTION OF NONOMURAEA DESERTI SP. NOV. Nonomuraea deserti sp. nov. (de.ser ′ ti. L. gen. n. deserti of a desert, referring to the source of the type strain). Aerobic, Gram-stain-positive, non-motile actinobacterium that forms extensively branched substrate and aerial mycelia. Aerial hyphae differentiated to hooked chains of spores with a smooth surfaces. Good growth occurs on ISP 2, 3, modified Bennett’s and nutrient agar and moderate growth on ISP 4–7, Czapek’s and tryptic soy agar, substrate mycelia may be brown, cream or yellow. Does not produce diffusible or melanoid pigments. Grows from 28–37 °C (optimum, 28 °C), from pH 6.0–8.0 (optimum, pH 7.0) and in the presence of up to 3 % (w/v) NaCl. Hydrolyses aesculin and arbutin, reduces nitrate but does not form H 2 S and does not hydrolyse allantoin or urea. Gelatin, hypoxanthine and xylan are degraded, but not adenine, casein, chitin, guanine, starch, Tweens 40 or 80 or xanthine. D-Arabinose, cellobiose, dextran, D-fructose, D-glucose, lactose, D-mannitol and raffinose are utilized as sole carbon and energy sources, but not adonitol, L-arabinose, dextrin, D-galactose, L-glutamine, maltose, myo-inositol, D-mannose, melezitose, melibiose, inulin, L-rhamnose, D-sorbitol, L-sorbose, sodium succinate, sucrose, xylitol or xylose. L-Arginine, L-asparagine, L-cysteine, glycine, L-hydroxyproline, L-methionine, L-phenylalanine, L-proline, *Data taken from Le Roes and Meyers [77]. †Data taken from Qin et al. [18]. ‡Data taken from Li et al. [29]. L-serine, L-tyrosine and L-valine are utilized as sole nitrogen sources, but not L-alanine, L-histidine or α-iso-leucine. The predominant menaquinones are MK-9(H 4) and MK-9(H 6). The polar lipid profile includes diphosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, hydroxy-phosphatidylethanolamine, dihydroxy-phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, three unidentified glycophospholipids, an unidentified phospholipid, an unidentified glycolipid and four unidentified lipids. Whole-cell hydrolysates contain meso -A 2 pm, glucose, mannose, madurose, ribose and galactose. The major fatty acids are iso-C 16:0, iso - C 16:0 2OH and C 17:0 10-methyl. The DNA G+C content of strain KC310 T is 70.9 mol% and the genome size 9.69 Mbp. The type strain, KC 310 T (= CGMCC 4.7331 T = DSM 102919 T =KCTC 39774 T), was isolated from desert soil sample collected from the Karakum Desert, Turkmenistan. The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain KC310 T is MG770639 and draħ genome sequence accession number SMKO00000000., Published as part of Saygin, Hayrettin, Nouioui, Imen, Ay, Hilal, Guven, Kiymet, Cetin, Demet, Klenk, Hans-Peter, Goodfellow, Michael & Sahin, Nevzat, 2020, Polyphasic classification of Nonomuraea strains isolated from the Karakum Desert and description of Nonomuraea deserti sp. nov., Nonomuraea diastatica sp. nov., Nonomuraea longispora sp. nov. and Nonomuraea mesophila sp. nov., pp. 636-647 in International Journal of Systematic and Evolutionary Microbiology 70 (1) on pages 641-644, DOI: 10.1099/ijsem.0.003808, http://zenodo.org/record/3745026, {"references":["77. Roes Mle, Meyers PR. Nonomuraea candida sp. nov., a new species from South African soil. Antonie van Leeuwenhoek 2008; 93: 133 - 139.","18. Qin S, Zhao GZ, Klenk HP, Li J, Zhu WY et al. Nonomuraea antimicrobica sp. nov., an endophytic actinomycete isolated from a leaf of Maytenus austroyunnanensis. Int J Syst Evol Microbiol 2009; 59: 2747 - 2751.","29. Li X, Zhang L, Ding Y, Gao Y, Ruan J et al. Nonomuraea jiangxiensis sp. nov., isolated from acidic soil. Int J Syst Evol Microbiol 2012; 62: 1409 - 1413."]}