17 results on '"Gutman, Martin J."'
Search Results
2. Induction of Transient Virus Replication Facilitates Antigen-Independent Isolation of SIV-Specific Monoclonal Antibodies
- Author
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Pedreño-Lopez, Nuria, primary, Dang, Christine M., additional, Rosen, Brandon C., additional, Ricciardi, Michael J., additional, Bailey, Varian K., additional, Gutman, Martin J., additional, Gonzalez-Nieto, Lucas, additional, Pauthner, Matthias G., additional, Le, Khoa, additional, Song, Ge, additional, Andrabi, Raiees, additional, Weisgrau, Kim L., additional, Pomplun, Nicholas, additional, Martinez-Navio, José M., additional, Fuchs, Sebastian P., additional, Wrammert, Jens, additional, Rakasz, Eva G., additional, Lifson, Jeffrey D., additional, Martins, Mauricio A., additional, Burton, Dennis R., additional, Watkins, David I., additional, and Magnani, Diogo M., additional
- Published
- 2020
- Full Text
- View/download PDF
3. The Frequency of Vaccine-Induced T-Cell Responses Does Not Predict the Rate of Acquisition after Repeated Intrarectal SIVmac239 Challenges inMamu-B*08 +Rhesus Macaques
- Author
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Martins, Mauricio A., primary, Gonzalez-Nieto, Lucas, additional, Shin, Young C., additional, Domingues, Aline, additional, Gutman, Martin J., additional, Maxwell, Helen S., additional, Magnani, Diogo M., additional, Ricciardi, Michael J., additional, Pedreño-Lopez, Núria, additional, Bailey, Varian K., additional, Altman, John D., additional, Parks, Christopher L., additional, Allison, David B., additional, Ejima, Keisuke, additional, Rakasz, Eva G., additional, Capuano, Saverio, additional, Desrosiers, Ronald C., additional, Lifson, Jeffrey D., additional, and Watkins, David I., additional
- Published
- 2019
- Full Text
- View/download PDF
4. Analysis of Simian Immunodeficiency Virus-specific CD8+ T-cells in Rhesus Macaques by Peptide-MHC-I Tetramer Staining
- Author
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Gonzalez-Nieto, Lucas, Domingues, Aline, Ricciardi, Michael, Gutman, Martin J., Maxwell, Helen S., Pedreño-Lopez, Nuria, Bailey, Varian, Magnani, Diogo M., and Martins, Mauricio A.
- Subjects
Staining and Labeling ,Immunology ,Histocompatibility Antigens Class I ,Animals ,Simian Immunodeficiency Virus ,CD8-Positive T-Lymphocytes ,Flow Cytometry ,Peptides ,Macaca mulatta ,Immunophenotyping - Abstract
Peptide-major histocompatibility complex class I (pMHC-I) tetramers have been an invaluable tool to study CD8+ T-cell responses. Because these reagents directly bind to T-cell receptors on the surface of CD8+ T-lymphocytes, fluorochrome-labeled pMHC-I tetramers enable the accurate detection of antigen (Ag)-specific CD8+ T-cells without the need for in vitro re-stimulation. Moreover, when combined with multi-color flow cytometry, pMHC-I tetramer staining can reveal key aspects of Ag-specific CD8+ T-cells, including differentiation stage, memory phenotype, and activation status. These types of analyses have been especially useful in the field of HIV immunology where CD8+ T-cells can affect progression to AIDS. Experimental infection of rhesus macaques with simian immunodeficiency virus (SIV) provides an invaluable tool to study cellular immunity against the AIDS virus. As a result, considerable progress has been made in defining and characterizing T-cell responses in this animal model. Here we present an optimized protocol for enumerating SIV-specific CD8+ T-cells in rhesus macaques by pMHC-I tetramer staining. Our assay permits the simultaneous quantification and memory phenotyping of two pMHC-I tetramer+ CD8+ T-cell populations per test, which might be useful for tracking SIV-specific CD8+ T-cell responses generated by vaccination or SIV infection. Considering the relevance of nonhuman primates in biomedical research, this methodology is applicable for studying CD8+ T-cell responses in multiple disease settings.
- Published
- 2016
5. Mamu-B*17 + Rhesus Macaques Vaccinated with env , vif , and nef Manifest Early Control of SIVmac239 Replication
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Martins, Mauricio A., primary, Tully, Damien C., additional, Pedreño-Lopez, Núria, additional, von Bredow, Benjamin, additional, Pauthner, Matthias G., additional, Shin, Young C., additional, Yuan, Maoli, additional, Lima, Noemia S., additional, Bean, David J., additional, Gonzalez-Nieto, Lucas, additional, Domingues, Aline, additional, Gutman, Martin J., additional, Maxwell, Helen S., additional, Magnani, Diogo M., additional, Ricciardi, Michael J., additional, Bailey, Varian K., additional, Altman, John D., additional, Burton, Dennis R., additional, Ejima, Keisuke, additional, Allison, David B., additional, Evans, David T., additional, Rakasz, Eva G., additional, Parks, Christopher L., additional, Bonaldo, Myrna C., additional, Capuano, Saverio, additional, Lifson, Jeffrey D., additional, Desrosiers, Ronald C., additional, Allen, Todd M., additional, and Watkins, David I., additional
- Published
- 2018
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- View/download PDF
6. Fetal demise and failed antibody therapy during Zika virus infection of pregnant macaques
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Magnani, Diogo M., primary, Rogers, Thomas F., additional, Maness, Nicholas J., additional, Grubaugh, Nathan D., additional, Beutler, Nathan, additional, Bailey, Varian K., additional, Gonzalez-Nieto, Lucas, additional, Gutman, Martin J., additional, Pedreño-Lopez, Núria, additional, Kwal, Jaclyn M., additional, Ricciardi, Michael J., additional, Myers, Tereance A., additional, Julander, Justin G., additional, Bohm, Rudolf P., additional, Gilbert, Margaret H., additional, Schiro, Faith, additional, Aye, Pyone P., additional, Blair, Robert V., additional, Martins, Mauricio A., additional, Falkenstein, Kathrine P., additional, Kaur, Amitinder, additional, Curry, Christine L., additional, Kallas, Esper G., additional, Desrosiers, Ronald C., additional, Goldschmidt-Clermont, Pascal J., additional, Whitehead, Stephen S., additional, Andersen, Kristian G., additional, Bonaldo, Myrna C., additional, Lackner, Andrew A., additional, Panganiban, Antonito T., additional, Burton, Dennis R., additional, and Watkins, David I., additional
- Published
- 2018
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7. Ontogeny of the B- and T-cell response in a primary Zika virus infection of a dengue-naïve individual during the 2016 outbreak in Miami, FL
- Author
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Ricciardi, Michael J., primary, Magnani, Diogo M., additional, Grifoni, Alba, additional, Kwon, Young-Chan, additional, Gutman, Martin J., additional, Grubaugh, Nathan D., additional, Gangavarapu, Karthik, additional, Sharkey, Mark, additional, Silveira, Cassia G. T., additional, Bailey, Varian K., additional, Pedreño-Lopez, Núria, additional, Gonzalez-Nieto, Lucas, additional, Maxwell, Helen S., additional, Domingues, Aline, additional, Martins, Mauricio A., additional, Pham, John, additional, Weiskopf, Daniela, additional, Altman, John, additional, Kallas, Esper G., additional, Andersen, Kristian G., additional, Stevenson, Mario, additional, Lichtenberger, Paola, additional, Choe, Hyeryun, additional, Whitehead, Stephen S., additional, Sette, Alessandro, additional, and Watkins, David I., additional
- Published
- 2017
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8. Potent Plasmablast-Derived Antibodies Elicited by the National Institutes of Health Dengue Vaccine
- Author
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Magnani, Diogo M., primary, Silveira, Cassia G. T., additional, Ricciardi, Michael J., additional, Gonzalez-Nieto, Lucas, additional, Pedreño-Lopez, Núria, additional, Bailey, Varian K., additional, Gutman, Martin J., additional, Maxwell, Helen S., additional, Domingues, Aline, additional, Costa, Priscilla R., additional, Ferrari, Lilian, additional, Goulart, Raphaella, additional, Martins, Mauricio A., additional, Martinez-Navio, José M., additional, Fuchs, Sebastian P., additional, Kalil, Jorge, additional, Timenetsky, Maria do Carmo, additional, Wrammert, Jens, additional, Whitehead, Stephen S., additional, Burton, Dennis R., additional, Desrosiers, Ronald C., additional, Kallas, Esper G., additional, and Watkins, David I., additional
- Published
- 2017
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9. Dengue Virus Evades AAV-Mediated Neutralizing Antibody Prophylaxis in Rhesus Monkeys
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Magnani, Diogo M., primary, Ricciardi, Michael J., additional, Bailey, Varian K., additional, Gutman, Martin J., additional, Pedreño-Lopez, Núria, additional, Silveira, Cassia G.T., additional, Maxwell, Helen S., additional, Domingues, Aline, additional, Gonzalez-Nieto, Lucas, additional, Su, Qin, additional, Newman, Ruchi M., additional, Pack, Melissa, additional, Martins, Mauricio A., additional, Martinez-Navio, José M., additional, Fuchs, Sebastian P., additional, Rakasz, Eva G., additional, Allen, Todd M., additional, Whitehead, Stephen S., additional, Burton, Dennis R., additional, Gao, Guangping, additional, Desrosiers, Ronald C., additional, Kallas, Esper G., additional, and Watkins, David I., additional
- Published
- 2017
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10. Rare Control of SIVmac239 Infection in a Vaccinated Rhesus Macaque
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Martins, Mauricio A., primary, Tully, Damien C., additional, Shin, Young C., additional, Gonzalez-Nieto, Lucas, additional, Weisgrau, Kim L., additional, Bean, David J., additional, Gadgil, Rujuta, additional, Gutman, Martin J., additional, Domingues, Aline, additional, Maxwell, Helen S., additional, Magnani, Diogo M., additional, Ricciardi, Michael, additional, Pedreño-Lopez, Nuria, additional, Bailey, Varian, additional, Cruz, Michael A., additional, Lima, Noemia S., additional, Bonaldo, Myrna C., additional, Altman, John D., additional, Rakasz, Eva, additional, Capuano, Saverio, additional, Reimann, Keith A., additional, Piatak, Michael, additional, Lifson, Jeffrey D., additional, Desrosiers, Ronald C., additional, Allen, Todd M., additional, and Watkins, David I., additional
- Published
- 2017
- Full Text
- View/download PDF
11. Vaccine-induced immune responses against both Gag and Env improve control of simian immunodeficiency virus replication in rectally challenged rhesus macaques
- Author
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Martins, Mauricio A., primary, Shin, Young C., additional, Gonzalez-Nieto, Lucas, additional, Domingues, Aline, additional, Gutman, Martin J., additional, Maxwell, Helen S., additional, Castro, Iris, additional, Magnani, Diogo M., additional, Ricciardi, Michael, additional, Pedreño-Lopez, Nuria, additional, Bailey, Varian, additional, Betancourt, Dillon, additional, Altman, John D., additional, Pauthner, Matthias, additional, Burton, Dennis R., additional, von Bredow, Benjamin, additional, Evans, David T., additional, Yuan, Maoli, additional, Parks, Christopher L., additional, Ejima, Keisuke, additional, Allison, David B., additional, Rakasz, Eva, additional, Barber, Glen N., additional, Capuano, Saverio, additional, Lifson, Jeffrey D., additional, Desrosiers, Ronald C., additional, and Watkins, David I., additional
- Published
- 2017
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12. A human inferred germline antibody binds to an immunodominant epitope and neutralizes Zika virus
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Magnani, Diogo M., primary, Silveira, Cassia G. T., additional, Rosen, Brandon C., additional, Ricciardi, Michael J., additional, Pedreño-Lopez, Núria, additional, Gutman, Martin J., additional, Bailey, Varian K., additional, Maxwell, Helen S., additional, Domingues, Aline, additional, Gonzalez-Nieto, Lucas, additional, Avelino-Silva, Vivian I., additional, Trindade, Mateus, additional, Nogueira, Juliana, additional, Oliveira, Consuelo S., additional, Maestri, Alvino, additional, Felix, Alvina Clara, additional, Levi, José Eduardo, additional, Nogueira, Mauricio L., additional, Martins, Mauricio A., additional, Martinez-Navio, José M., additional, Fuchs, Sebastian P., additional, Whitehead, Stephen S., additional, Burton, Dennis R., additional, Desrosiers, Ronald C., additional, Kallas, Esper G., additional, and Watkins, David I., additional
- Published
- 2017
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13. Analysis of Simian Immunodeficiency Virus-specific CD8+ T-cells in Rhesus Macaques by Peptide-MHC-I Tetramer Staining
- Author
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Gonzalez-Nieto, Lucas, primary, Domingues, Aline, primary, Ricciardi, Michael, primary, Gutman, Martin J., primary, Maxwell, Helen S., primary, Pedreño-Lopez, Nuria, primary, Bailey, Varian, primary, Magnani, Diogo M., primary, and Martins, Mauricio A., primary
- Published
- 2016
- Full Text
- View/download PDF
14. The Frequency of Vaccine-Induced T-Cell Responses Does Not Predict the Rate of Acquisition after Repeated Intrarectal SIVmac239 Challenges in Mamu-B*08+ Rhesus Macaques.
- Author
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Martins, Mauricio A., Gonzalez-Nieto, Lucas, Shin, Young C., Domingues, Aline, Gutman, Martin J., Maxwell, Helen S., Magnani, Diogo M., Ricciardi, Michael J., Pedreño-Lopez, Núria, Bailey, Varian K., Altman, John D., Parks, Christopher L., Allison, David B., Ejima, Keisuke, Rakasz, Eva G., Capuano III, Saverio, Desrosiers, Ronald C., Lifson, Jeffrey D., and Watkins, David I.
- Subjects
- *
RHESUS monkeys , *SIMIAN immunodeficiency virus diseases vaccines , *T cells , *CD8 antigen , *HIV infections - Abstract
Approximately 50% of rhesus macaques (RMs) expressing the major histocompatibility complex class I (MHC-I) allele Mamu-B*08 spontaneously control chronic-phase viremia after infection with the pathogenic simian immunodeficiency virus mac239 (SIVmac239) clone. CD8+ T-cell responses in these animals are focused on immunodominant Mamu-B*08-restricted SIV epitopes in Vif and Nef, and prophylactic vaccination with these epitopes increases the incidence of elite control in SIVmac239-infected Mamu-B*08-positive (Mamu-B*08+) RMs. Here we evaluated if robust vaccine-elicited CD8+ T-cell responses against Vif and Nef can prevent systemic infection in Mamu-B*08+ RMs following mucosal SIV challenges. Ten Mamu-B*08+ RMs were vaccinated with a heterologous prime/boost/boost regimen encoding Vif and Nef, while six sham-vaccinated MHC-I-matched RMs served as the controls for this experiment. Vaccine-induced CD8+ T cells against Mamu-B*08-restricted SIV epitopes reached high frequencies in blood but were present at lower levels in lymph node and gut biopsy specimens. Following repeated intrarectal challenges with SIVmac239, all control RMs became infected by the sixth SIV exposure. By comparison, four vaccinees were still uninfected after six challenges, and three of them remained aviremic after 3 or 4 additional challenges. The rate of SIV acquisition in the vaccinees was numerically lower (albeit not statistically significantly) than that in the controls. However, peak viremia was significantly reduced in infected vaccinees compared to control animals. We found no T-cell markers that distinguished vaccinees that acquired SIV infection from those that did not. Additional studies will be needed to validate these findings and determine if cellular immunity can be harnessed to prevent the establishment of productive immunodeficiency virus infection. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
15. Mamu-B*17+ Rhesus Macaques Vaccinated with env, vif, and nef Manifest Early Control of SIVmac239 Replication.
- Author
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Martins, Mauricio A., Tully, Damien C., Pedreño-Lopez, Núria, Von Bredow, Benjamin, Pauthner, Matthias G., Shin, Young C., Yuan, Maoli, Lima, Noemia S., Bean, David J., Gonzalez-Nieto, Lucas, Domingues, Aline, Gutman, Martin J., Maxwell, Helen S., Magnani, Diogo M., Ricciardi, Michael J., Bailey, Varian K., Altman, John D., Burton, Dennis R., Ejima, Keisuke, and Allison, David B.
- Subjects
- *
RHESUS monkeys , *MAJOR histocompatibility complex , *ANIMAL vaccination , *SIMIAN immunodeficiency virus diseases , *HIV prevention - Abstract
Certain major histocompatibility complex class I (MHC-I) alleles are associated with spontaneous control of viral replication in human immunodeficiency virus (HIV)-infected people and simian immunodeficiency virus (SIV)- infected rhesus macaques (RMs). These cases of "elite" control of HIV/SIV replication are often immune-mediated, thereby providing a framework for studying anti-lentiviral immunity. In this study, we examined how vaccination impacts SIV replication in RMs expressing the MHC-I allele Mamu-B*17. Approximately 21% of Mamu-B*17+ and 50% of Mamu-B*08+ RMs control chronic-phase viremia after SIVmac239 infection. Because CD8+ T cells targeting Mamu-B*08-restricted SIV epitopes have been implicated in virologic suppression in Mamu-B*08+ RMs, we investigated whether this might also be true for Mamu-B*17+ RMs. Two groups of Mamu-B*17+ RMs were vaccinated with genes encoding Mamu-B*17-restricted epitopes in Vif and Nef. These genes were delivered by themselves (group 1) or together with env (group 2). Group 3 included MHC-I-matched RMs and served as the control group. Surprisingly, the group 1 vaccine regimen had little effect on viral replication compared to group 3, suggesting that unlike Mamu-B*08+ RMs, preexisting SIV-specific CD8+ T cells alone do not facilitate long-term virologic suppression in Mamu-B*17+ RMs. Remarkably, however, 5/8 group 2 vaccinees controlled viremia to <15 viral RNA copies/ml soon after infection. No serological neutralizing activity against SIVmac239 was detected in group 2, although vaccine-elicited gp140-binding antibodies correlated inversely with nadir viral loads. Collectively, these data shed new light on the unique mechanism of elite control in Mamu-B*17+ RMs and implicate vaccine-induced, nonneutralizing anti- Env antibodies in the containment of immunodeficiency virus infection. [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
- View/download PDF
16. Induction of Transient Virus Replication Facilitates Antigen-Independent Isolation of SIV-Specific Monoclonal Antibodies.
- Author
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Pedreño-Lopez N, Dang CM, Rosen BC, Ricciardi MJ, Bailey VK, Gutman MJ, Gonzalez-Nieto L, Pauthner MG, Le K, Song G, Andrabi R, Weisgrau KL, Pomplun N, Martinez-Navio JM, Fuchs SP, Wrammert J, Rakasz EG, Lifson JD, Martins MA, Burton DR, Watkins DI, and Magnani DM
- Abstract
Structural characterization of the HIV-1 Envelope (Env) glycoprotein has facilitated the development of Env probes to isolate HIV-specific monoclonal antibodies (mAbs). However, preclinical studies have largely evaluated these virus-specific mAbs against chimeric viruses, which do not naturally infect non-human primates, in contrast to the unconstrained simian immunodeficiency virus (SIV)mac239 clone. Given the paucity of native-like reagents for the isolation of SIV-specific B cells, we examined a method to isolate SIVmac239-specific mAbs without using Env probes. We first activated virus-specific B cells by inducing viral replication after the infusion of a CD8β-depleting mAb or withdrawal of antiretroviral therapy in SIVmac239-infected rhesus macaques. Following the rise in viremia, we observed 2- to 4-fold increases in the number of SIVmac239 Env-reactive plasmablasts in circulation. We then sorted these activated B cells and obtained 206 paired Ab sequences. After expressing 122 mAbs, we identified 14 Env-specific mAbs. While these Env-specific mAbs bound to both the SIVmac239 SOSIP.664 trimer and to infected primary rhesus CD4
+ T cells, five also neutralized SIVmac316. Unfortunately, none of these mAbs neutralized SIVmac239. Our data show that this method can be used to isolate virus-specific mAbs without antigenic probes by inducing bursts of contemporary replicating viruses in vivo ., (© 2020 The Author(s).)- Published
- 2020
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- View/download PDF
17. The Frequency of Vaccine-Induced T-Cell Responses Does Not Predict the Rate of Acquisition after Repeated Intrarectal SIVmac239 Challenges in Mamu-B*08 + Rhesus Macaques.
- Author
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Martins MA, Gonzalez-Nieto L, Shin YC, Domingues A, Gutman MJ, Maxwell HS, Magnani DM, Ricciardi MJ, Pedreño-Lopez N, Bailey VK, Altman JD, Parks CL, Allison DB, Ejima K, Rakasz EG, Capuano S 3rd, Desrosiers RC, Lifson JD, and Watkins DI
- Subjects
- Animals, Epitopes, T-Lymphocyte immunology, Gene Products, nef administration & dosage, Gene Products, vif administration & dosage, Histocompatibility Antigens Class I immunology, Macaca mulatta, Vaccination, Viral Vaccines immunology, Viremia immunology, CD8-Positive T-Lymphocytes immunology, Gene Products, nef immunology, Gene Products, vif immunology, SAIDS Vaccines immunology, Simian Acquired Immunodeficiency Syndrome immunology, Simian Immunodeficiency Virus immunology
- Abstract
Approximately 50% of rhesus macaques (RMs) expressing the major histocompatibility complex class I (MHC-I) allele Mamu-B*08 spontaneously control chronic-phase viremia after infection with the pathogenic simian immunodeficiency virus mac239 (SIVmac239) clone. CD8
+ T-cell responses in these animals are focused on immunodominant Mamu-B*08-restricted SIV epitopes in Vif and Nef, and prophylactic vaccination with these epitopes increases the incidence of elite control in SIVmac239-infected Mamu-B*08 -positive ( Mamu-B*08+ ) RMs. Here we evaluated if robust vaccine-elicited CD8+ T-cell responses against Vif and Nef can prevent systemic infection in Mamu-B*08+ RMs following mucosal SIV challenges. Ten Mamu-B*08+ RMs were vaccinated with a heterologous prime/boost/boost regimen encoding Vif and Nef, while six sham-vaccinated MHC-I-matched RMs served as the controls for this experiment. Vaccine-induced CD8+ T cells against Mamu-B*08-restricted SIV epitopes reached high frequencies in blood but were present at lower levels in lymph node and gut biopsy specimens. Following repeated intrarectal challenges with SIVmac239, all control RMs became infected by the sixth SIV exposure. By comparison, four vaccinees were still uninfected after six challenges, and three of them remained aviremic after 3 or 4 additional challenges. The rate of SIV acquisition in the vaccinees was numerically lower (albeit not statistically significantly) than that in the controls. However, peak viremia was significantly reduced in infected vaccinees compared to control animals. We found no T-cell markers that distinguished vaccinees that acquired SIV infection from those that did not. Additional studies will be needed to validate these findings and determine if cellular immunity can be harnessed to prevent the establishment of productive immunodeficiency virus infection. IMPORTANCE It is generally accepted that the antiviral effects of vaccine-induced classical CD8+ T-cell responses against human immunodeficiency virus (HIV) are limited to partial reductions in viremia after the establishment of productive infection. Here we show that rhesus macaques (RMs) vaccinated with Vif and Nef acquired simian immunodeficiency virus (SIV) infection at a lower (albeit not statistically significant) rate than control RMs following repeated intrarectal challenges with a pathogenic SIV clone. All animals in the present experiment expressed the elite control-associated major histocompatibility complex class I (MHC-I) molecule Mamu-B*08 that binds immunodominant epitopes in Vif and Nef. Though preliminary, these results provide tantalizing evidence that the protective efficacy of vaccine-elicited CD8+ T cells may be greater than previously thought. Future studies should examine if vaccine-induced cellular immunity can prevent systemic viral replication in RMs that do not express MHC-I alleles associated with elite control of SIV infection., (Copyright © 2019 American Society for Microbiology.)- Published
- 2019
- Full Text
- View/download PDF
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