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5 results on '"Gutierrez-Beltran, Emilio [0000-0001-7978-3164]"'

1. A proxitome-RNA-capture approach reveals that processing bodies repress coregulated hub genes

Author

Carl Tryggers Foundation, Helge Ax:son Johnsons Stiftelse, Hellenic Foundation for Research & Innovation, European Commission, Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Junta de Andalucía, Foundation for Research and Technology, Swedish Research Council for Sustainable Development, Kempestiftelserna, Liu, Chen [0000-0002-1604-0694], Mentzelopoulou, Andriani [0000-0003-4266-8714], Hatzianestis, Ioannis H [0009-0009-0213-775X], Tzagkarakis, Epameinondas [0009-0008-5615-2296], Skaltsogiannis, Vasileios [0000-0003-4155-9540], Ma, Xuemin [0000-0001-5690-7929], Michalopoulou, Vassiliki A [0000-0002-7663-2472], Romero-Campero, Francisco J. [0000-0001-9834-030X], Romero-Losada, Ana B [0000-0003-4073-3631], Sarris, Panagiotis F [0000-0001-7000-8997], Marhavy, Peter [0000-0002-0178-2230], Bölter, Bettina [0000-0002-7780-7289], Kanterakis, Alexandros [0000-0003-4276-0115], Gutierrez-Beltran, Emilio [0000-0001-7978-3164], Moschou, Panagiotis N [0000-0001-7212-0595], Liu, Chen, Mentzelopoulou, Andriani, Hatzianestis, Ioannis H, Tzagkarakis, Epameinondas, Skaltsogiannis, Vasileios, Ma, Xuemin, Michalopoulou, Vassiliki A, Romero-Campero, Francisco J., Romero-Losada, Ana B, Sarris, Panagiotis F, Marhavy, Peter, Bölter, Bettina, Kanterakis, Alexandros, Gutierrez-Beltran, Emilio, Moschou, Panagiotis N, Carl Tryggers Foundation, Helge Ax:son Johnsons Stiftelse, Hellenic Foundation for Research & Innovation, European Commission, Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Junta de Andalucía, Foundation for Research and Technology, Swedish Research Council for Sustainable Development, Kempestiftelserna, Liu, Chen [0000-0002-1604-0694], Mentzelopoulou, Andriani [0000-0003-4266-8714], Hatzianestis, Ioannis H [0009-0009-0213-775X], Tzagkarakis, Epameinondas [0009-0008-5615-2296], Skaltsogiannis, Vasileios [0000-0003-4155-9540], Ma, Xuemin [0000-0001-5690-7929], Michalopoulou, Vassiliki A [0000-0002-7663-2472], Romero-Campero, Francisco J. [0000-0001-9834-030X], Romero-Losada, Ana B [0000-0003-4073-3631], Sarris, Panagiotis F [0000-0001-7000-8997], Marhavy, Peter [0000-0002-0178-2230], Bölter, Bettina [0000-0002-7780-7289], Kanterakis, Alexandros [0000-0003-4276-0115], Gutierrez-Beltran, Emilio [0000-0001-7978-3164], Moschou, Panagiotis N [0000-0001-7212-0595], Liu, Chen, Mentzelopoulou, Andriani, Hatzianestis, Ioannis H, Tzagkarakis, Epameinondas, Skaltsogiannis, Vasileios, Ma, Xuemin, Michalopoulou, Vassiliki A, Romero-Campero, Francisco J., Romero-Losada, Ana B, Sarris, Panagiotis F, Marhavy, Peter, Bölter, Bettina, Kanterakis, Alexandros, Gutierrez-Beltran, Emilio, and Moschou, Panagiotis N

Abstract

Cellular condensates are usually ribonucleoprotein assemblies with liquid- or solid-like properties. Because these subcellular structures lack a delineating membrane, determining their compositions is difficult. Here we describe a proximity-biotinylation approach for capturing the RNAs of the condensates known as processing bodies (PBs) in Arabidopsis (Arabidopsis thaliana). By combining this approach with RNA detection, in silico, and high-resolution imaging approaches, we studied PBs under normal conditions and heat stress. PBs showed a much more dynamic RNA composition than the total transcriptome. RNAs involved in cell wall development and regeneration, plant hormonal signaling, secondary metabolism/defense, and RNA metabolism were enriched in PBs. RNA-binding proteins and the liquidity of PBs modulated RNA recruitment, while RNAs were frequently recruited together with their encoded proteins. In PBs, RNAs follow distinct fates: in small liquid-like PBs, RNAs get degraded while in more solid-like larger ones, they are stored. PB properties can be regulated by the actin-polymerizing SCAR (suppressor of the cyclic AMP)-WAVE (WASP family verprolin homologous) complex. SCAR/WAVE modulates the shuttling of RNAs between PBs and the translational machinery, thereby adjusting ethylene signaling. In summary, we provide an approach to identify RNAs in condensates that allowed us to reveal a mechanism for regulating RNA fate.

Published
2024

2. Arabidopsis metacaspase MC1 localizes in stress granules, clears protein aggregates, and delays senescence

Author

Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Ministerio de Economía y Competitividad (España), European Commission, Generalitat de Catalunya, Ministerio de Universidades (España), German Research Foundation, Else Kröner-Fresenius Foundation, European Cooperation in Science and Technology, CSIC - Unidad de Recursos de Información Científica para la Investigación (URICI), Ruiz-Solaní, Nerea [0000-0002-0813-0956], Salguero-Linares, José Manuel [0000-0003-0559-7793], Armengot, Laia [0000-0002-3790-9838], Santos, Jaime [0000-0001-9045-7765], Pallarès, Irantzu [0000-0002-8205-2060], Midden, Katarina P. [0000-0002-4962-5410], Phukkan, Ujjal J. [0000-0001-9788-4938], Koyuncu, Seda [0000-0001-7937-3974], Borràs-Bisa, Júlia [0009-0009-0096-9990], Li, Liang [0009-0003-2816-1885], Popa, Crina [0000-0002-4294-9949], Eisele, Frederik [0000-0001-9995-5816], Eisele-Bürger, Anna Maria [0000-0002-4009-094X], Hill, S. M. [0000-0001-7969-097X], Gutierrez-Beltran, Emilio [0000-0001-7978-3164], Nyström, Thomas [0000-0001-5489-2903], Valls, Marc [0000-0003-2312-0091], Llamas, Ernesto [0000-0001-9262-2402], Vilchez, David [0000-0002-0801-0743], Klemenčič, Marina [0000-0002-3079-4492], Ventura, Salvador [0000-0002-9652-6351], Coll, Núria S. [0000-0002-8889-0399], Ruiz-Solaní, Nerea, Salguero-Linares, José Manuel, Armengot, Laia, Santos, Jaime, Pallarès, Irantzu, Midden, Katarina P. van, Phukkan, Ujjal J., Koyuncu, Seda, Borràs-Bisa, Júlia, Li, Liang, Popa, Crina, Eisele, Frederik, Eisele-Bürger, Anna Maria, Hill, S. M., Gutierrez-Beltran, Emilio, Nyström, Thomas, Valls, Marc, Llamas, Ernesto, Vilchez, David, Klemenčič, Marina, Ventura, Salvador, Coll, Núria S., Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Ministerio de Economía y Competitividad (España), European Commission, Generalitat de Catalunya, Ministerio de Universidades (España), German Research Foundation, Else Kröner-Fresenius Foundation, European Cooperation in Science and Technology, CSIC - Unidad de Recursos de Información Científica para la Investigación (URICI), Ruiz-Solaní, Nerea [0000-0002-0813-0956], Salguero-Linares, José Manuel [0000-0003-0559-7793], Armengot, Laia [0000-0002-3790-9838], Santos, Jaime [0000-0001-9045-7765], Pallarès, Irantzu [0000-0002-8205-2060], Midden, Katarina P. [0000-0002-4962-5410], Phukkan, Ujjal J. [0000-0001-9788-4938], Koyuncu, Seda [0000-0001-7937-3974], Borràs-Bisa, Júlia [0009-0009-0096-9990], Li, Liang [0009-0003-2816-1885], Popa, Crina [0000-0002-4294-9949], Eisele, Frederik [0000-0001-9995-5816], Eisele-Bürger, Anna Maria [0000-0002-4009-094X], Hill, S. M. [0000-0001-7969-097X], Gutierrez-Beltran, Emilio [0000-0001-7978-3164], Nyström, Thomas [0000-0001-5489-2903], Valls, Marc [0000-0003-2312-0091], Llamas, Ernesto [0000-0001-9262-2402], Vilchez, David [0000-0002-0801-0743], Klemenčič, Marina [0000-0002-3079-4492], Ventura, Salvador [0000-0002-9652-6351], Coll, Núria S. [0000-0002-8889-0399], Ruiz-Solaní, Nerea, Salguero-Linares, José Manuel, Armengot, Laia, Santos, Jaime, Pallarès, Irantzu, Midden, Katarina P. van, Phukkan, Ujjal J., Koyuncu, Seda, Borràs-Bisa, Júlia, Li, Liang, Popa, Crina, Eisele, Frederik, Eisele-Bürger, Anna Maria, Hill, S. M., Gutierrez-Beltran, Emilio, Nyström, Thomas, Valls, Marc, Llamas, Ernesto, Vilchez, David, Klemenčič, Marina, Ventura, Salvador, and Coll, Núria S.

Abstract

Stress granules (SGs) are highly conserved cytoplasmic condensates that assemble in response to stress and contribute to maintaining protein homeostasis. These membraneless organelles are dynamic, disassembling once the stress is no longer present. Persistence of SGs due to mutations or chronic stress has been often related to age-dependent protein-misfolding diseases in animals. Here, we find that the metacaspase MC1 is dynamically recruited into SGs upon proteotoxic stress in Arabidopsis (Arabidopsis thaliana). Two predicted disordered regions, the prodomain and the 360 loop, mediate MC1 recruitment to and release from SGs. Importantly, we show that MC1 has the capacity to clear toxic protein aggregates in vivo and in vitro, acting as a disaggregase. Finally, we demonstrate that overexpressing MC1 delays senescence and this phenotype is dependent on the presence of the 360 loop and an intact catalytic domain. Together, our data indicate that MC1 regulates senescence through its recruitment into SGs and this function could potentially be linked to its remarkable protein aggregate-clearing activity.

Published
2023

3. Phenolic acid-induced phase separation and translation inhibition mediate plant interspecific competition

Author

National Natural Science Foundation of China, Department of Agriculture (US), European Commission, Natural Science Foundation of Fujian Province, Zhao, Shuai [0000-0002-8482-452X], Shi, Yabo [0000-0002-0062-7909], Chen, Xiaoyuan [0009-0009-1665-8900], Wang, Xingwei [0000-0002-6437-7187], Gutierrez-Beltran, Emilio [0000-0001-7978-3164], Bozhkov, Peter V [0000-0002-8819-3884], Qian, Weiqiang [0000-0002-3135-6689], Zhou, Mian [0000-0002-9332-0147], Wang, Wei [0000-0002-3780-5158], Xie, Zhouli, Zhao, Shuai, Li, Ying, Deng, Yuhua, Shi, Yabo, Chen, Xiaoyuan, Li, Yue, Li, Haiwei, Chen, Changtian, Wang, Xingwei, Liu, Enhui, Tu, Yuchen, Shi, Peng, Tong, Jinjin, Gutierrez-Beltran, Emilio, Li, Jiayu, Bozhkov, Peter V, Qian, Weiqiang, Zhou, Mian, Wang, Wei, National Natural Science Foundation of China, Department of Agriculture (US), European Commission, Natural Science Foundation of Fujian Province, Zhao, Shuai [0000-0002-8482-452X], Shi, Yabo [0000-0002-0062-7909], Chen, Xiaoyuan [0009-0009-1665-8900], Wang, Xingwei [0000-0002-6437-7187], Gutierrez-Beltran, Emilio [0000-0001-7978-3164], Bozhkov, Peter V [0000-0002-8819-3884], Qian, Weiqiang [0000-0002-3135-6689], Zhou, Mian [0000-0002-9332-0147], Wang, Wei [0000-0002-3780-5158], Xie, Zhouli, Zhao, Shuai, Li, Ying, Deng, Yuhua, Shi, Yabo, Chen, Xiaoyuan, Li, Yue, Li, Haiwei, Chen, Changtian, Wang, Xingwei, Liu, Enhui, Tu, Yuchen, Shi, Peng, Tong, Jinjin, Gutierrez-Beltran, Emilio, Li, Jiayu, Bozhkov, Peter V, Qian, Weiqiang, Zhou, Mian, and Wang, Wei

Abstract

Phenolic acids (PAs) secreted by donor plants suppress the growth of their susceptible plant neighbours. However, how structurally diverse ensembles of PAs are perceived by plants to mediate interspecific competition remains a mystery. Here we show that a plant stress granule (SG) marker, RNA-BINDING PROTEIN 47B (RBP47B), is a sensor of PAs in Arabidopsis. PAs, including salicylic acid, 4-hydroxybenzoic acid, protocatechuic acid and so on, directly bind RBP47B, promote its phase separation and trigger SG formation accompanied by global translation inhibition. Salicylic acid-induced global translation inhibition depends on RBP47 family members. RBP47s regulate the proteome rather than the absolute quantity of SG. The rbp47 quadruple mutant shows a reduced sensitivity to the inhibitory effect of the PA mixture as well as to that of PA-rich rice when tested in a co-culturing ecosystem. In this Article, we identified the long sought-after PA sensor as RBP47B and illustrated that PA-induced SG-mediated translational inhibition was one of the PA perception mechanisms.

Published
2023

4. Stress-related biomolecular condensates in plants

Author

Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Junta de Andalucía, Ministry of Development (Greece), European Commission, Swedish Research Council for Sustainable Development, Hellenic Foundation for Research and Innovation, Knut and Alice Wallenberg Foundation, Carl Trygger Foundation, Swedish Research Council, Solis-Miranda, Jorge [0000-0001-7140-2558], Chodasiewicz, Monika [0000-0002-0295-0638], Skirycz, Aleksandra [0000-0002-7627-7925], Fernie, Alisdair R [0000-0001-9000-335X], Moschou, Panagiotis N [0000-0001-7212-0595], Bozhkov, Peter V [0000-0002-8819-3884], Gutierrez-Beltran, Emilio [0000-0001-7978-3164], Solis-Miranda, Jorge, Chodasiewicz, Monika, Skirycz, Aleksandra, Fernie, Alisdair R, Moschou, Panagiotis N, Bozhkov, Peter V, Gutierrez-Beltran, Emilio, Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Junta de Andalucía, Ministry of Development (Greece), European Commission, Swedish Research Council for Sustainable Development, Hellenic Foundation for Research and Innovation, Knut and Alice Wallenberg Foundation, Carl Trygger Foundation, Swedish Research Council, Solis-Miranda, Jorge [0000-0001-7140-2558], Chodasiewicz, Monika [0000-0002-0295-0638], Skirycz, Aleksandra [0000-0002-7627-7925], Fernie, Alisdair R [0000-0001-9000-335X], Moschou, Panagiotis N [0000-0001-7212-0595], Bozhkov, Peter V [0000-0002-8819-3884], Gutierrez-Beltran, Emilio [0000-0001-7978-3164], Solis-Miranda, Jorge, Chodasiewicz, Monika, Skirycz, Aleksandra, Fernie, Alisdair R, Moschou, Panagiotis N, Bozhkov, Peter V, and Gutierrez-Beltran, Emilio

Abstract

Biomolecular condensates are membraneless organelle-like structures that can concentrate molecules and often form through liquid-liquid phase separation. Biomolecular condensate assembly is tightly regulated by developmental and environmental cues. Although research on biomolecular condensates has intensified in the past 10 years, our current understanding of the molecular mechanisms and components underlying their formation remains in its infancy, especially in plants. However, recent studies have shown that the formation of biomolecular condensates may be central to plant acclimation to stress conditions. Here, we describe the mechanism, regulation, and properties of stress-related condensates in plants, focusing on stress granules and processing bodies, 2 of the most well-characterized biomolecular condensates. In this regard, we showcase the proteomes of stress granules and processing bodies in an attempt to suggest methods for elucidating the composition and function of biomolecular condensates. Finally, we discuss how biomolecular condensates modulate stress responses and how they might be used as targets for biotechnological efforts to improve stress tolerance.

Published
2023

5. An actin remodeling role for Arabidopsis processing bodies revealed by their proximity interactome

Author

European Commission, Carl Tryggers Foundation, Swedish Research Council for Sustainable Development, Helge Ax:son Johnsons Stiftelse, Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Liu, Chen [0000-0002-1604-0694], Mentzelopoulou, Andriani [0000-0003-4266-8714], Weijers, Dolf [0000-0003-4378-141X], Gutierrez-Beltran, Emilio [0000-0001-7978-3164], Moschou, Panagiotis N [0000-0001-7212-0595], Liu, Chen, Mentzelopoulou, Andriani, Muhammad, Amna, Volkov, Andriy, Weijers, Dolf, Gutierrez-Beltran, Emilio, Moschou, Panagiotis N, European Commission, Carl Tryggers Foundation, Swedish Research Council for Sustainable Development, Helge Ax:son Johnsons Stiftelse, Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Liu, Chen [0000-0002-1604-0694], Mentzelopoulou, Andriani [0000-0003-4266-8714], Weijers, Dolf [0000-0003-4378-141X], Gutierrez-Beltran, Emilio [0000-0001-7978-3164], Moschou, Panagiotis N [0000-0001-7212-0595], Liu, Chen, Mentzelopoulou, Andriani, Muhammad, Amna, Volkov, Andriy, Weijers, Dolf, Gutierrez-Beltran, Emilio, and Moschou, Panagiotis N

Abstract

Cellular condensates can comprise membrane-less ribonucleoprotein assemblies with liquid-like properties. These cellular condensates influence various biological outcomes, but their liquidity hampers their isolation and characterization. Here, we investigated the composition of the condensates known as processing bodies (PBs) in the model plant Arabidopsis thaliana through a proximity-biotinylation proteomics approach. Using in situ protein-protein interaction approaches, genetics and high-resolution dynamic imaging, we show that processing bodies comprise networks that interface with membranes. Surprisingly, the conserved component of PBs, DECAPPING PROTEIN 1 (DCP1), can localize to unique plasma membrane subdomains including cell edges and vertices. We characterized these plasma membrane interfaces and discovered a developmental module that can control cell shape. This module is regulated by DCP1, independently from its role in decapping, and the actin-nucleating SCAR-WAVE complex, whereby the DCP1-SCAR-WAVE interaction confines and enhances actin nucleation. This study reveals an unexpected function for a conserved condensate at unique membrane interfaces.

Published
2023

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