16 results on '"Gustavo Capistrano"'
Search Results
2. Noninvasive intratumoral thermal dose determination during in vivo magnetic nanoparticle hyperthermia: combining surface temperature measurements and computer simulations
- Author
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Gustavo Capistrano, Harley F. Rodrigues, Nicholas Zufelato, Cristhiane Gonçalves, Clever G. Cardoso, Elisangela P. Silveira-Lacerda, and Andris F. Bakuzis
- Subjects
thermal nanomedicine ,fluorescence molecular tomography ,photogrammetry ,treatment planning ,cancer ,Medical technology ,R855-855.5 - Abstract
Purpose Noninvasive thermometry during magnetic nanoparticle hyperthermia (MNH) remains a challenge. Our pilot study proposes a methodology to determine the noninvasive intratumoral thermal dose during MNH in the subcutaneous tumor model. Methods Two groups of Ehrlich bearing-mice with solid and subcutaneous carcinoma, a control group (n = 6), and a MNH treated group (n = 4) were investigated. Histopathology was used to evaluate the percentage of non-viable lesions in the tumor. MNH was performed at 301 kHz and 17.5 kA.m−1, using a multifunctional nanocarrier. Surface temperature measurements were obtained using an infrared camera, where an ROI with 750 pixels was used for comparison with computer simulations. Realistic simulations of the bioheat equation were obtained by combining histopathology intratumoral lesion information and surface temperature agreement of at least 50% of the pixel’s temperature data calculated and measured at the surface. Results One animal of the MNH group showed tumor recurrence, while two others showed complete tumor remission (monitored for 585 days). Sensitivity analysis of the simulation parameters indicated low tumor blood perfusion. Numerical simulations indicated, for the animals with complete remission, an irreversible tissue injury of 91 ± 5% and 100%, while the one with recurrence had a lower value, 56 ± 7%. The computer simulations also revealed the in vivo heat efficiency of the nanocarrier. Conclusion A new methodology for determining noninvasively the three-dimensional intratumoral thermal dose during MNH was developed. The method demonstrates the potential for predicting the long-term preclinical outcome of animals treated with MNH.
- Published
- 2020
- Full Text
- View/download PDF
3. New Prospects in Neutering Male Animals Using Magnetic Nanoparticle Hyperthermia
- Author
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José Luiz P. R. Jivago, Juliana Lis Mendes Brito, Gustavo Capistrano, Marcus Vinícius-Araújo, Ediron Lima Verde, Andris Figueiroa Bakuzis, Paulo E. N. Souza, Ricardo Bentes Azevedo, and Carolina Madeira Lucci
- Subjects
infertility ,testicles ,spermatogenesis ,magnetohyperthermia ,nanocontraception ,Pharmacy and materia medica ,RS1-441 - Abstract
Controlling populations of free-roaming dogs and cats poses a huge challenge worldwide. Non-surgical neutering strategies for male animals have been long pursued, but the implementation of the procedures developed has remained limited to date. As submitting the testes to high temperatures impairs spermatogenesis, the present study investigated localized application of magnetic nanoparticle hyperthermia (MNH) to the testicles as a potential non-surgical sterilization method for animals. An intratesticular injection of a magnetic fluid composed of manganese-ferrite nanoparticles functionalized with citrate was administered followed by testicle exposure to an alternate magnetic field to generate localized heat. Testicular MNH was highly effective, causing progressive seminiferous tubule degeneration followed by substitution of the parenchyma with stromal tissue and gonadal atrophy, suggesting an irreversible process with few side effects to general animal health.
- Published
- 2021
- Full Text
- View/download PDF
4. Noninvasive intratumoral thermal dose determination during in vivo magnetic nanoparticle hyperthermia: combining surface temperature measurements and computer simulations
- Author
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Clever Gomes Cardoso, Cristhiane Goncalves, Elisangela de Paula Silveira-Lacerda, Andris F. Bakuzis, Nicholas Zufelato, Harley F Rodrigues, and Gustavo Capistrano
- Subjects
Hyperthermia ,treatment planning ,Cancer Research ,lcsh:Medical technology ,Materials science ,Physiology ,Nanoparticle ,photogrammetry ,Temperature measurement ,030218 nuclear medicine & medical imaging ,03 medical and health sciences ,0302 clinical medicine ,In vivo ,Physiology (medical) ,Noninvasive thermometry ,medicine ,cancer ,fluorescence molecular tomography ,Fluorescence molecular tomography ,equipment and supplies ,medicine.disease ,lcsh:R855-855.5 ,thermal nanomedicine ,030220 oncology & carcinogenesis ,Thermal dose ,human activities ,Biomedical engineering - Abstract
Purpose Noninvasive thermometry during magnetic nanoparticle hyperthermia (MNH) remains a challenge. Our pilot study proposes a methodology to determine the noninvasive intratumoral thermal dose during MNH in the subcutaneous tumor model. Methods Two groups of Ehrlich bearing-mice with solid and subcutaneous carcinoma, a control group (n = 6), and a MNH treated group (n = 4) were investigated. Histopathology was used to evaluate the percentage of non-viable lesions in the tumor. MNH was performed at 301 kHz and 17.5 kA.m−1, using a multifunctional nanocarrier. Surface temperature measurements were obtained using an infrared camera, where an ROI with 750 pixels was used for comparison with computer simulations. Realistic simulations of the bioheat equation were obtained by combining histopathology intratumoral lesion information and surface temperature agreement of at least 50% of the pixel’s temperature data calculated and measured at the surface. Results One animal of the MNH group showed tumor recurrence, while two others showed complete tumor remission (monitored for 585 days). Sensitivity analysis of the simulation parameters indicated low tumor blood perfusion. Numerical simulations indicated, for the animals with complete remission, an irreversible tissue injury of 91 ± 5% and 100%, while the one with recurrence had a lower value, 56 ± 7%. The computer simulations also revealed the in vivo heat efficiency of the nanocarrier. Conclusion A new methodology for determining noninvasively the three-dimensional intratumoral thermal dose during MNH was developed. The method demonstrates the potential for predicting the long-term preclinical outcome of animals treated with MNH.
- Published
- 2020
5. Predictive Model for Delivery Efficiency: Erythrocyte Membrane-Camouflaged Magnetofluorescent Nanocarriers Study
- Author
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José Ricardo de Arruda Miranda, Gustavo Capistrano, Ailton A. Sousa-Junior, Emílio Ramos Cintra, Sônia F. O. Santos, Sebastião Antonio Mendanha, Clever Gomes Cardoso, Antonio Alonso, Guilherme Augusto Soares, Marcus S. Carrião, Andris F. Bakuzis, Nicholas Zufelato, Eliana Martins Lima, Elisângela de Paula Silveira-Lacerda, André G. Próspero, Universidade Federal de Goiás (UFG), and Universidade Estadual Paulista (Unesp)
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Biodistribution ,photothermal therapy ,Photothermal Therapy ,tumor delivery efficiency ,Pharmaceutical Science ,02 engineering and technology ,Ferric Compounds ,030226 pharmacology & pharmacy ,Theranostic Nanomedicine ,Mice ,03 medical and health sciences ,0302 clinical medicine ,Pharmacokinetics ,In vivo ,Drug Discovery ,Tumor Microenvironment ,Animals ,Tissue Distribution ,magnetic hyperthermia ,Particle Size ,Carcinoma, Ehrlich Tumor ,Fluorescent Dyes ,Drug Carriers ,Tumor microenvironment ,near-infrared dye ,Chemistry ,Erythrocyte Membrane ,membrane-coated nanoparticles ,Hyperthermia, Induced ,Photothermal therapy ,021001 nanoscience & nanotechnology ,Tumor Burden ,Disease Models, Animal ,Magnetic hyperthermia ,Manganese Compounds ,Magnets ,pharmacokinetic model ,Biophysics ,Molecular Medicine ,Female ,Magnetic Iron Oxide Nanoparticles ,iron oxide-based nanoparticles ,Nanocarriers ,0210 nano-technology ,Ex vivo - Abstract
Made available in DSpace on 2020-12-12T01:17:17Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-03-02 Delivery efficiencies of theranostic nanoparticles (NPs) based on passive tumor targeting strongly depend either on their blood circulation time or on appropriate modulations of the tumor microenvironment. Therefore, predicting the NP delivery efficiency before and after a tumor microenvironment modulation is highly desirable. Here, we present a new erythrocyte membrane-camouflaged magnetofluorescent nanocarrier (MMFn) with long blood circulation time (92 h) and high delivery efficiency (10% ID for Ehrlich murine tumor model). MMFns owe their magnetic and fluorescent properties to the incorporation of manganese ferrite nanoparticles (MnFe2O4 NPs) and IR-780 (a lipophilic indocyanine fluorescent dye), respectively, to their erythrocyte membrane-derived camouflage. MMFn composition, morphology, and size, as well as optical absorption, zeta potential, and fluorescent, magnetic, and magnetothermal properties, are thoroughly examined in vitro. We then present an analytical pharmacokinetic (PK) model capable of predicting the delivery efficiency (DE) and the time of peak tumor uptake (tmax), as well as changes in DE and tmax due to modulations of the tumor microenvironment, for potentially any nanocarrier. Experimental PK data sets (blood and tumor amounts of MMFns) are simultaneously fit to the model equations using the PK modeling software Monolix. We then validate our model analytical solutions with the numerical solutions provided by Monolix. We also demonstrate how our a priori nonmechanistic model for passive targeting relates to a previously reported mechanistic model for active targeting. All in vivo PK studies, as well as in vivo and ex vivo biodistribution studies, were conducted using two noninvasive techniques, namely, fluorescence molecular tomography (FMT) and alternating current biosusceptometry (ACB). Finally, histopathology corroborates our PK and biodistribution results. Physics Institute Federal University of Goiás Biomagnetism Lab Physics and Biophysics Department São Paulo State University Laboratory of Pharmaceutical Nanotechnology and Drug Delivery Systems School of Pharmacy Federal University of Goiás Biological Sciences Institute Federal University of Goiás Biomagnetism Lab Physics and Biophysics Department São Paulo State University
- Published
- 2020
6. New Prospects in Neutering Male Animals Using Magnetic Nanoparticle Hyperthermia
- Author
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Ricardo Bentes Azevedo, Paulo E. N. Souza, Juliana Lis Mendes de Brito, Carolina Madeira Lucci, Andris F. Bakuzis, E. L. Verde, Gustavo Capistrano, Marcus Vinícius-Araújo, and José Luiz P. R. Jivago
- Subjects
Hyperthermia ,Pathology ,medicine.medical_specialty ,Stromal cell ,Nanopartículas magnéticas ,Pharmaceutical Science ,Testicle ,Infertilidade ,Article ,Pharmacy and materia medica ,Parenchyma ,medicine ,Hipertermia ,Castração ,CATS ,business.industry ,testicles ,magnetohyperthermia ,Testículos ,medicine.disease ,spermatogenesis ,RS1-441 ,Neutering ,Seminiferous tubule ,medicine.anatomical_structure ,nanocontraception ,business ,Espermatogênese ,infertility ,Spermatogenesis - Abstract
Controlling populations of free-roaming dogs and cats poses a huge challenge worldwide. Non-surgical neutering strategies for male animals have been long pursued, but the implementation of the procedures developed has remained limited to date. As submitting the testes to high temperatures impairs spermatogenesis, the present study investigated localized application of magnetic nanoparticle hyperthermia (MNH) to the testicles as a potential non-surgical sterilization method for animals. An intratesticular injection of a magnetic fluid composed of manganese-ferrite nanoparticles functionalized with citrate was administered followed by testicle exposure to an alternate magnetic field to generate localized heat. Testicular MNH was highly effective, causing progressive seminiferous tubule degeneration followed by substitution of the parenchyma with stromal tissue and gonadal atrophy, suggesting an irreversible process with few side effects to general animal health.
- Published
- 2021
7. IR-780-Albumin-Based Nanocarriers Promote Tumor Regression Not Only from Phototherapy but Also by a Nonirradiation Mechanism
- Author
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Maristela Pereira, Gustavo Capistrano, Clever Gomes Cardoso, Ailton A. Sousa-Junior, Emílio Ramos Cintra, Sônia F. O. Santos, Andris F. Bakuzis, Eliana Martins Lima, Nicholas Zufelato, Francyelli Mello-Andrade, Carlos Henrique de Castro, Sebastião Antonio Mendanha, André Luiz Silva Oliveira, Allancer D C Nunes, Elisângela de Paula Silveira-Lacerda, Roosevelt Alves da Silva, and Raisa Melo Lima
- Subjects
Biodistribution ,Indoles ,medicine.medical_treatment ,0206 medical engineering ,Biomedical Engineering ,Photodynamic therapy ,02 engineering and technology ,Pharmacology ,Ehrlich ascites carcinoma ,Biomaterials ,chemistry.chemical_compound ,Mice ,In vivo ,Cell Line, Tumor ,medicine ,Animals ,Tissue Distribution ,Bovine serum albumin ,biology ,Lonidamine ,Hyperthermia, Induced ,Photothermal therapy ,Phototherapy ,021001 nanoscience & nanotechnology ,020601 biomedical engineering ,Molecular Docking Simulation ,chemistry ,biology.protein ,Nanocarriers ,0210 nano-technology - Abstract
IR-780 iodide is a fluorescent dye with optical properties in the near-infrared region that has applications in tumor detection and photothermal/photodynamic therapy. This multifunctional effect led to the development of theranostic nanoparticles with both IR-780 and chemotherapeutic drugs such as docetaxel, doxorubicin, and lonidamine. In this work, we developed two albumin-based nanoparticles containing near-infrared IR-780 iodide multifunctional dyes, one of them possessing a magnetic core. Molecular docking with AutoDock Vina studies showed that IR-780 binds to bovine serum albumin (BSA) with greater stability at a higher temperature, allowing the protein binding pocket to better fit this dye. The theoretical analysis corroborates the experimental protocols, where an enhancement of IR-780 was found coupled to BSA at 60 °C, even 30 days after preparation, in comparison to 30 °C. In vitro assays monitoring the viability of Ehrlich ascites carcinoma cells revealed the importance of the inorganic magnetic core on the nanocarrier photothermal-cytotoxic effect. Fluorescence molecular tomography measurements of Ehrlich tumor-bearing Swiss mice revealed the biodistribution of the nanocarriers, with marked accumulation in the tumor tissue (≈3% ID). The histopathological analysis demonstrated strong increase in tumoral necrosis areas after 24 and 72 h after treatment, indicating tumor regression. Tumor regression analysis of nonirradiated animals indicate a IR-780 dose-dependent antitumoral effect with survival rates higher than 70% (animals monitored up to 600 days). Furthermore, an in vivo photothermal therapy procedure was performed and tumor regression was also verified. These results show a novel insight for the biomedical application of IR-780-albumin-based nanocarriers, namely cancer therapy, not only by photoinduced therapy but also by a nonirradiation mechanism. Safety studies (acute oral toxicity, cardiovascular evaluation, and histopathological analysis) suggest potential for clinical translation.
- Published
- 2021
8. Noninvasive intratumoral thermal dose determination during
- Author
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Gustavo, Capistrano, Harley F, Rodrigues, Nicholas, Zufelato, Cristhiane, Gonçalves, Clever G, Cardoso, Elisangela P, Silveira-Lacerda, and Andris F, Bakuzis
- Subjects
Mice ,Temperature ,Animals ,Computer Simulation ,Hyperthermia ,Pilot Projects ,Hyperthermia, Induced ,Neoplasm Recurrence, Local ,Magnetite Nanoparticles - Abstract
Noninvasive thermometry during magnetic nanoparticle hyperthermia (MNH) remains a challenge. Our pilot study proposes a methodology to determine the noninvasive intratumoral thermal dose during MNH in the subcutaneous tumor model.Two groups of Ehrlich bearing-mice with solid and subcutaneous carcinoma, a control group (One animal of the MNH group showed tumor recurrence, while two others showed complete tumor remission (monitored for 585 days). Sensitivity analysis of the simulation parameters indicated low tumor blood perfusion. Numerical simulations indicated, for the animals with complete remission, an irreversible tissue injury of 91 ± 5% and 100%, while the one with recurrence had a lower value, 56 ± 7%. The computer simulations also revealed theA new methodology for determining noninvasively the three-dimensional intratumoral thermal dose during MNH was developed. The method demonstrates the potential for predicting the long-term preclinical outcome of animals treated with MNH.
- Published
- 2021
9. Elaboration of magneto-thermally recyclable nanosorbents for remote removal of toluene in contaminated water using magnetic hyperthermia
- Author
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Marcelo Henrique Sousa, A. F. C. Campos, Andris F. Bakuzis, Juliano de Andrade Gomes, Gustavo Capistrano, Fernando F. Sodré, Fernanda L. Rodovalho, and Juliano A. Chaker
- Subjects
Aqueous solution ,Materials science ,General Chemical Engineering ,Analytical chemistry ,Langmuir adsorption model ,02 engineering and technology ,General Chemistry ,010501 environmental sciences ,021001 nanoscience & nanotechnology ,01 natural sciences ,Toluene ,Industrial and Manufacturing Engineering ,symbols.namesake ,chemistry.chemical_compound ,Magnetic hyperthermia ,Adsorption ,Chemical engineering ,chemistry ,Desorption ,symbols ,Environmental Chemistry ,Fourier transform infrared spectroscopy ,0210 nano-technology ,High-resolution transmission electron microscopy ,0105 earth and related environmental sciences - Abstract
Magnetic nanosorbents are novel materials that have been impacting various applications in environmental fields, including the detection of pollutants and remediation of polluted environments. In this work, the thermal heating effect caused by the interaction of nanoparticles’ magnetic moments with an alternating magnetic field (AMF) – magnetic hyperthermia – is proposed as proof of a concept to illustrate the remote controlled recycling of magnetic nanosorbents during the treatment of water containing toluene, a potentially toxic and volatile contaminant. Nanosorbents were synthesized by functionalization of ∼15 nm coprecipitated cobalt/manganese mixed ferrite with polydimethylsiloxane. Structural, chemical, and magnetic characteristics of the nanosized adsorbent were investigated by elemental analysis (ICP-OES), X-ray diffractometry (XRD), high resolution transmission electron microscopy (HRTEM), Fourier transform infrared spectroscopy (FTIR) and vibrating sample magnetometry (VSM). Batch experiments indicated that nanoadsorbents, which can be magnetically recovered, exhibit high adsorption affinity for toluene in aqueous solution (∼325 mg/g) and that adsorption follows the Langmuir model. Moreover, application of AMF resulted in uniform heating within the nanosorbents (after being magnetically separated from the contaminated water) and triggered toluene desorption/evaporation, leading to accelerated release of pollutants from the nanosorbent, improving its reusability; even after seven cycles the removal efficiency was quite high (83%).
- Published
- 2016
10. Precise determination of the heat delivery during in vivo magnetic nanoparticle hyperthermia with infrared thermography
- Author
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Elisângela de Paula Silveira-Lacerda, Francyelli Mariana dos Santos Mello, Harley F Rodrigues, Andris F. Bakuzis, Nicholas Zufelato, and Gustavo Capistrano
- Subjects
Hyperthermia ,Male ,Work (thermodynamics) ,Materials science ,Hot Temperature ,Infrared ,Infrared Rays ,Nanoparticle ,02 engineering and technology ,Ferric Compounds ,03 medical and health sciences ,Mice ,0302 clinical medicine ,In vivo ,Neoplasms ,medicine ,Dosimetry ,Animals ,Radiology, Nuclear Medicine and imaging ,Radiological and Ultrasound Technology ,Temperature ,Hyperthermia, Induced ,021001 nanoscience & nanotechnology ,medicine.disease ,Magnetic hyperthermia ,Manganese Compounds ,Thermography ,030220 oncology & carcinogenesis ,Magnets ,Nanoparticles ,0210 nano-technology ,Biomedical engineering - Abstract
Non-invasive and real-time monitoring of the heat delivery during magnetic nanoparticle hyperthermia (MNH) is of fundamental importance to predict clinical outcomes for cancer treatment. Infrared thermography (IRT) can determine the surface temperature due to three-dimensional heat delivery inside a subcutaneous tumor, an argument that is supported by numerical simulations. However, for precise temperature determination, it is of crucial relevance to use a correct experimental configuration. This work reports an MNH study using a sarcoma 180 murine tumor containing 3.9 mg of intratumorally injected manganese-ferrite nanoparticles. MNH was performed at low field amplitude and non-uniform field configuration. Five 30 min in vivo magnetic hyperthermia experiments were performed, monitoring the surface temperature with a fiber optical sensor and thermal camera at distinct angles with respect to the animal's surface. The results indicate that temperature errors as large as [Formula: see text]C can occur if the experiment is not properly designed. A new IRT error model is found to explain the data. More importantly, we show how to precisely monitor temperature with IRT during hyperthermia, which could positively impact heat dosimetry and clinical planning.
- Published
- 2017
11. IR-780-Albumin-Based Nanocarriers Promote Tumor Regression Not Only from Phototherapy but Also by a Nonirradiation Mechanism.
- Author
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Gustavo, Capistrano, Ailton A., Sousa-Junior, Roosevelt A., Silva, Francyelli, Mello-Andrade, Emilio R., Cintra, Sônia, Santos, Allancer D., Nunes, Raisa M., Lima, Nicholas, Zufelato, André S., Oliveira, Maristela, Pereira, Carlos H., Castro, Eliana M., Lima, Clever G., Cardoso, Elisângela, Silveira-Lacerda, Sebastião A., Mendanha, and Andris F., Bakuzis
- Published
- 2020
- Full Text
- View/download PDF
12. Elementos traço e parasitismo em tilápia do nilo cultivada no sul do Brasil
- Author
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Gustavo Capistrano Nunes, Maurício Laterça Martins, Gabriela Tomas Jerônimo, Luiz Rodrigo Mota Vicente, Rubens Riscala Madi, and Gustavo Moraes Ramos Valladão
- Subjects
0301 basic medicine ,Veterinary medicine ,Ecology ,Fish farming ,Fluorescence spectrometry ,chemistry.chemical_element ,Zinc ,030108 mycology & parasitology ,Aquatic Science ,Biology ,biology.organism_classification ,Manure ,Mercury (element) ,03 medical and health sciences ,Nile tilapia ,chemistry ,Animal Science and Zoology ,Monoculture ,Arsenic - Abstract
This study evaluated the trace elements and parasitological indices in Nile tilapia examined from two different facilities, named as swine-consorted, using pig manure, and monoculture. For trace element analysis, the fish muscle tissue was collected individually in each facility. Each portion was weighed, dried in a stove at 60°C for 48 h and analyzed by Energy Dispersive X-ray Fluorescence Spectrometry (EDXRF). Parasitological analysis followed the routine method for parasites collection, quantification and identification. The predominant element was zinc (Zn) followed by iron (Fe) and arsenic (As) in fish from both facilities. Fish from swine-consorted system had greater values of abundance and mean intensity of trichodinids and fish from monoculture showed higher abundance and mean intensity of monogeneans. Nevertheless, no signs of damage to fish production was observed. The contents of the elements Zn, Fe, As, cooper (Cu) and mercury (Hg) were above of the maximum permitted limits recommended by the Brazilian legislation. © 2016, Instytut Technologii Drewna. All rights reserved.
- Published
- 2016
13. Pharmacokinetic model for quantitative evaluation of the epr effect on albumin-based nanocarriers
- Author
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Francyelli Mello Andrade, Wanessa C. Pires, Gustavo Capistrano Pinto Leite, Ailton A. Sousa Junior, Andris F. Bakuzis, Nicholas Zufelato, and Elisângela de Paula Silveira Lacerda
- Subjects
Chromatography ,Pharmacokinetics ,law ,Chemistry ,Albumin ,Nanocarriers ,Electron paramagnetic resonance ,law.invention - Published
- 2018
14. Elementos traço e parasitismo em tilápia do Nilo cultivada na região sul de Santa Catarina, estudo de caso
- Author
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Nunes, Gustavo Capistrano, Universidade Federal de Santa Catarina, Martins, Maurício Laterça, and Jerônimo, Gabriela Tomas
- Subjects
Aquicultura ,Criação ,Elementos tracos ,Parasitismo ,Tilápia (Peixe) ,Bioacumulacao ,Santa Catarina, Sul - Abstract
Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro de Ciências Agrárias, Programa de Pós-Graduação em Aquicultura, Florianópolis, 2015. Abstract : Due to possible risks for human health, in the last decades, several studies have been realized in evaluating the fish capacity in concentrate trace elements in the internal organs and muscle. This study verifies the bioaccumulation of trace elements and parasitological indexes of 120 Nile tilapia examined in two different properties, named as ?Swine-consorted? using pig manure, and ?Semi intensive?. For trace element analysis, the fish muscle tissue was collected individually in each property. Each portion was weighed, dried in oven at 60°C for 48 h. and analyzed by the fluorescence spectrometry of X rays by energy dispersion (EDXRF). Parasitological analysis followed the routine method for parasites collection, quantification and identification. The predominant element was Zn followed by Fe and As in both properties. Parasitological analyses showed that fish from ?Swine-consorted? facility had greater values of abundance and mean intensity of trichodinids and in fish from ?Semi intensive? higher abundance and mean intensity of monogeneans was found. Nevertheless, no signs of injury to the creation was observed. The contents of the elements Zn, Fe, Cu, As and Hg were above the maximum limits recommended by the Brazilian legislation.
- Published
- 2015
15. Trace elements and parasitism in Nile tilapia farmed in the Southern Brazil
- Author
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NUNES, Gustavo Capistrano, primary, JERÔNIMO, Gabriela Tomas, additional, VICENTE, Luiz Rodrigo Mota, additional, MADI, Rubens Riscala, additional, VALLADÃO, Gustavo Moraes Ramos, additional, and MARTINS, Maurício Laterça, additional
- Published
- 2016
- Full Text
- View/download PDF
16. Precise determination of the heat delivery during in vivo magnetic nanoparticle hyperthermia with infrared thermography.
- Author
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Harley F Rodrigues, Gustavo Capistrano, Francyelli M Mello, Nicholas Zufelato, Elisângela Silveira-Lacerda, and Andris F Bakuzis
- Subjects
- *
MAGNETIC nanoparticle hyperthermia , *MEDICAL thermography , *CANCER treatment - Abstract
Non-invasive and real-time monitoring of the heat delivery during magnetic nanoparticle hyperthermia (MNH) is of fundamental importance to predict clinical outcomes for cancer treatment. Infrared thermography (IRT) can determine the surface temperature due to three-dimensional heat delivery inside a subcutaneous tumor, an argument that is supported by numerical simulations. However, for precise temperature determination, it is of crucial relevance to use a correct experimental configuration. This work reports an MNH study using a sarcoma 180 murine tumor containing 3.9 mg of intratumorally injected manganese-ferrite nanoparticles. MNH was performed at low field amplitude and non-uniform field configuration. Five 30 min in vivo magnetic hyperthermia experiments were performed, monitoring the surface temperature with a fiber optical sensor and thermal camera at distinct angles with respect to the animal’s surface. The results indicate that temperature errors as large as C can occur if the experiment is not properly designed. A new IRT error model is found to explain the data. More importantly, we show how to precisely monitor temperature with IRT during hyperthermia, which could positively impact heat dosimetry and clinical planning. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF
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