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Background: Decision-making alterations are present in psychiatric illnesses like major depressive disorder (MDD), obsessivecompulsive disorder (OCD), and schizophrenia, linked to symptoms of the respective disorders. We sought to analyze unique and shared decision-making alterations in these disorders, which is crucial for early diagnosis and treatment, especially given potential comorbidities. Methods: Using 2 computational modelling approaches--logistic regression and hierarchical Bayesian modelling--we analyzed alterations in model-based and model-free decision-making in a transdiagnostic cohort of patients with MDD, OCD, or schizophrenia. Our aim was to identify disorder-specific and shared alterations and their associations with symptoms. Results: We included 23 patients with MDD, 25 patients with OCD, 27 patients with schizophrenia, and 25 controls. Overall, participants of all groups relied on model-free decision-making. Patients with schizophrenia had the lowest learning rate and highest switching rate, indicating low perseverance. Furthermore, patients with OCD were more random in both task stages than controls and patients with MDD. All patient groups exhibited more randomness in responses than controls, with the schizophrenia group showing the highest levels. Increased model- free behaviour correlated with elevated depressive symptoms, and more model-based decision-making was linked to lower anhedonia levels across all patient groups. Limitations: The sample size in each group was small. Conclusion: This study highlights disorder-specific and shared decision-making alterations among people with MDD, OCD, or schizophrenia. Our findings suggest that anhedonia and depressive symptoms, which are present in all 3 disorders, share underlying behavioural mechanisms. Improving model-based behaviour may be a target for intervention and treatment. Furthermore, completely random behaviour in the 2-step task appears to distinctly differentiate patients with schizophrenia in remission., Introduction Decision-making and learning impairments are core characteristics of a wide range of psychiatric disorders--such as major depressive disorder (MDD), obsessive-compulsive disorder (OCD), and schizophrenia--and are linked to their specific [...]

This study examines the commitments of an alternative teacher education program in linking theory and practice, one of the most prominent problems of the field. This qualitative phenomenological study drew from semi-structured interviews with seven faculty members and associate instructors, electronic surveys with seven program students, participant observation and field notes in seminar sessions, one program faculty meeting, and one community meeting, and curriculum materials. The findings suggest that the design of the program and the roles and (inter)actions of the stakeholders within the program created a dialectical interplay. Linked with this interplay, Teachers' Society provided a setting for collaborative learning during which theory is extracted from experience.

Abstract With advanced mass spectrometry (MS)-based proteomics, genome-scale proteome coverage can be achieved from bulk tissues. However, such bulk measurement lacks spatial resolution and obscures tissue heterogeneity, precluding proteome mapping of tissue microenvironment. Here we report an integrated wet collection of single microscale tissue voxels and Surfactant-assisted One-Pot voxel processing method termed wcSOP for robust label-free single voxel proteomics. wcSOP capitalizes on buffer droplet-assisted wet collection of single voxels dissected by LCM to the tube cap and SOP voxel processing in the same collection cap. This method enables reproducible, label-free quantification of approximately 900 and 4600 proteins for single voxels at 20 µm × 20 µm × 10 µm (~1 cell region) and 200 µm × 200 µm × 10 µm (~100 cell region) from fresh frozen human spleen tissue, respectively. It can reveal spatially resolved protein signatures and region-specific signaling pathways. Furthermore, wcSOP-MS is demonstrated to be broadly applicable for OCT-embedded and FFPE human archived tissues as well as for small-scale 2D proteome mapping of tissues at high spatial resolutions. wcSOP-MS may pave the way for routine robust single voxel proteomics and spatial proteomics.

Red raspberries are among the most significant wild edible fruits (WEFs) in Türkiye, thriving in cold to warm temperate regions across the country. This study focuses on 11 wild red raspberry ecotypes sampled from the Northern Anatolia region, examining their sensory, biochemical and antioxidant properties. Sensory analysis was used to compare ecotypes based on aroma, taste and juiciness. Biochemical assessments included soluble solid content (SSC), vitamin C amount, organic acids, total anthocyanins (TA), total phenolic content (TPC), total flavonoid content and total antioxidant capacity. The Ferric Reducing Antioxidant Power (FRAP) assay measured antioxidant capacity. Despite similar growing conditions, significant variations were observed among ecotypes and across years. In the first year, fruit weights ranged from 1.04 g to 1.33 g, and in the second year, they ranged from 0.97 g to 1.27 g. Fruit chroma values ranged between 26.11 and 33.70 in 2021, and 23.17 and 30.19 in 2022. Vitamin C exhibited considerable variability, ranging from 29.3 mg · 100 g−1 to 44.4 mg · 100 g−1 across ecotypes and years. TPC, total anthocyanin content (TAC) and total flavonoid content ranged from 164 mg to 390 mg gallic acid equivalent (GAE) · 100 g−1, 17.3 mg to 33.2 mg cyanidin-3-glucoside equivalent · 100 g−1 and 10.3 mg to 17.6 mg quercetin equivalent (QE) · 100 g−1, respectively, in both years. Citric acid emerged as the dominant organic acid across all ecotypes. Notably, the ecotypes V-4, V-8, V-3 and V-10 showcased larger, more appealing fruits suitable for fresh consumption, whereas V-3 and V-5 presented sweeter fruits ideal for processing. Additionally, the ecotypes V-6, V-7 and V-11 displayed higher levels of health-promoting compounds, such as TPC and antioxidant capacity, suggesting their potential as functional foods and valuable sources of natural antioxidants in the future.

We have conducted a study to review and synthesize the current state of data availability for cradle-to-grave life-cycle emissions from major building technologies and electricity generation and storage technologies as specific to California as could be found. Results from 280 building technologies (120 unique) were organized across 9 categories and 27 subcategories. Many of the technologies in the list are common building materials, appliances, and process equipment used in the construction and operation of agricultural, residential, commercial, and industrial buildings. Target electricity generation technologies covered the GHG emissions from natural gas, solar, wind, geothermal, biomass and storage technologies for the California context. The search for relevant environmental impact data was in the form of Environmental Product Declarations (EPD) (if available), peer-reviewed journal articles, and publicly available reports from government and industry for each technology. In general, the “Building Materials” category in the building technologies area and “Wind Turbines” in the electricity generation and storage area have the most current and relevant data for California. However, we have identified several data gaps in our survey of the remaining categories. Due to lack of relevant data for California in building systems, there is an urgent need for policy makers and industry stakeholders to replicate policies such as AB 2446 to expand the coverage of availability of EPDs for products. Similarly, to achieve the SB 350 (Clean Energy and Pollution Reduction Act) goals and to support the state’s efforts to reduce GHG emissions by 80% below 1990 levels by the year 2050, we need to account for embodied emissions together with the other important life-cycle stages of renewable energy sources.

This study examines the potential strategies for reducing embodied energy and greenhouse gas emissions through adaptive reuse of non-residential buildings for residential purposes, as compared to new construction of apartment buildings. Such an approach can address housing crises in urban areas with an abundance of underutilized non-residential buildings, promoting sustainable housing growth. A comprehensive assessment of repurposing in California reveals approximately 510 million m² of floor space across 230,000 non-residential buildings in the current building stock. The potential reduction in embodied energy and CO2eq emissions ranges from 0.14 to 1.4 billion GJ and 5.0–70 million metric tons for the state, respectively, contingent upon the percentage of repurposed floor space (10–100%) and adaptive reuse scenario (retaining structural components and façade or solely the structure). A repurposed building avoids about 56% of embodied energy, 34-48% of CO2 eq emissions, and 72% of materials by mass compared to building a new apartment building. However, various technical, financial, and regulatory challenges may hinder emissions reductions, necessitating proactive policy measures. Cities can potentially expedite the process by streamlining approvals for mixed-use adaptive reuse projects involving both commercial and residential spaces.

While recognized as important, calculation of embodied energy and greenhouse gas (GHG) emissions associated with buildings, especially at a large scale, has scant literature. A model has been created for estimating the inventory of structural and non-structural materials and building components and their associated embodied energy and GHG emissions for the approximately 807,400 office buildings constructed in the United States between 1946 and 2018. The buildings were modeled using eight prototypical designs. We estimate that 1100–1300 million metric tons of materials are embodied in these 807,000 buildings (90% of which have just 1–3 floors), as well as 6–7 years' worth of national construction and demolition waste. In total, 6.5 billion Gigajoules of primary energy use (∼6% of the U.S.’s 2021 energy consumption) and 0.5 billion metric tons of carbon dioxide equivalent emissions (∼8% of the U.S.’s 2020 total GHG emissions) are estimated to be embodied in these buildings. One-floor steel and wood buildings were about equally GHG intensive from structural materials as well as combined structural and non-structural materials perspectives, while reinforced concrete (RC) buildings were 50% and 27%–47% more GHG intensive, respectively. From the all-materials-use perspective, 5-floor steel buildings were 54% more GHG intensive to construct than wood buildings, and in turn RC buildings were 68% more GHG intensive than steel buildings. Non-structural material contributions were significant. Increasing economies of scale in embodied impacts can be observed as the number of floors increases. Results constitute points of reference for those who seek to find ways of reducing the carbon footprint of buildings.

BackgroundLeishmaniasis, a parasitic infection affecting both humans and animals, is increasingly spreading across Mediterranean and European regions, largely driven by human migration and environmental changes. In countries like Türkiye and across Europe, which have seen large influxes of migrants, the incidence of cutaneous leishmaniasis (CL) is rising, with cases now appearing in cities where the disease was previously undocumented. In these previously non-endemic areas, physicians unfamiliar with the characteristic lesions may misdiagnose CL, particularly in cases with only cutaneous manifestations. This study aims to evaluate the impact of re-emerging CL on the routine diagnostic practices of pathologists in Türkiye, by retrospectively reviewing cases.MethodsWe conducted a retrospective analysis of CL cases diagnosed between 2013 and 2022 at a single pathology center in Türkiye, covering multiple provinces. Twelve cases of CL were identified and analyzed based on clinical presentation, pre-diagnosis, histopathological findings, and molecular diagnostics. DNA extraction and PCR were performed on paraffin-embedded tissue samples to identify the Leishmania species involved.ResultsOut of the twelve CL cases reviewed, seven exhibited morphological findings strongly suggestive of CL (MFSS of CL), warranting further microbiological evaluation. All patients presented with non-healing skin lesions characterized by central ulceration, crater-like formations, or papulonodular lesions. Notably, CL was included in the clinical pre-diagnosis in only 58.3% of cases, while it was not considered in the remaining 41.7% of cases. Clinicians initially pre-diagnosed skin tumors in six cases (50%), four of which led to wide surgical excision. Histopathological examination in all cases revealed chronic or mixed (acute/chronic) inflammation, predominantly rich in histiocytes. To further investigate the role of Leishmania species in the pre-diagnosis, DNA extraction and PCR were performed on paraffin-embedded tissue samples, identifying L. infantum as the causative agent in 10 cases and L. major in two cases. Notably, L. infantum was the causative agent in all five cases initially misdiagnosed as skin tumors, which were also associated with a granulomatous type of chronic inflammation.

This article reports a case study that examined the peace education practice of a 5th and 6th grade teacher at an independent, non-profit school in the Mid-western United States. The study used Paulo Freire's (1970) conception of dialogue as its conceptual framework. After describing the study's context and methods, we present data focusing on the teacher's background and development as a peace educator, her teaching practices, and her relationships with her students, school and local community. We discuss Michelle's interdisciplinary approach to peace education linked with her personal background and her use of dialogue as dynamic, fluid, and relational. Our analyses also prompted emergent themes for which we used curriculum theory to capture forms of integration between the personal and political dimensions of peace education, based on which, we propose an "integrated peace education."

This study emphasizes that research and practice regarding employing dialogue as transformative pedagogy should be investigated and cultivated by peace educators in ways relevant to various contexts. In this regard, Critical Discourse Analysis (CDA) has a valuable potential to contribute to the development of this relatively new scholarly field by providing effective tools to problematize and analyze social practices. This paper explores critical peace education as it is informed by the dialogical method of emancipatory education, and scrutinizes the promising potential of CDA as an essential tool for on-going research in the field. Towards this goal, this paper includes two subsections. The first section discusses Freirean dialogue and his six preconditions (i.e., love, humility, faith, hope, trust, and critical thinking) as fundamental constructs for critical peace education. The second section explores how the theories, goals, and methods of CDA in current discourse studies connect to constructs in Freirean dialogue and peace education. This section concentrates on four prominent approaches developed by Scollon, Gee, Kress, and Fairclough to underline their key aspects from a Freirian dialogic perspective. The final section discusses the possible affordances and limitations for employing CDA in the study of critical peace education.

Abstract The role of high mobility group box 1 (HMGB1) in inflammation is well characterized in the immune system and in response to tissue injury. More recently, HMGB1 was also shown to initiate an “inflammatory signaling cascade” in the brain parenchyma after a mild and brief disturbance, such as cortical spreading depolarization (CSD), leading to headache. Despite substantial evidence implying a role for inflammatory signaling in prevalent neuropsychiatric disorders such as migraine and depression, how HMGB1 is released from healthy neurons and how inflammatory signaling is initiated in the absence of apparent cell injury are not well characterized. We triggered a single cortical spreading depolarization by optogenetic stimulation or pinprick in naïve Swiss albino or transgenic Thy1-ChR2-YFP and hGFAP-GFP adult mice. We evaluated HMGB1 release in brain tissue sections prepared from these mice by immunofluorescent labeling and immunoelectron microscopy. EzColocalization and Costes thresholding algorithms were used to assess the colocalization of small extracellular vesicles (sEVs) carrying HMGB1 with astrocyte or microglia processes. sEVs were also isolated from the brain after CSD, and neuron-derived sEVs were captured by CD171 (L1CAM). sEVs were characterized with flow cytometry, scanning electron microscopy, nanoparticle tracking analysis, and Western blotting. We found that HMGB1 is released mainly within sEVs from the soma of stressed neurons, which are taken up by surrounding astrocyte processes. This creates conditions for selective communication between neurons and astrocytes bypassing microglia, as evidenced by activation of the proinflammatory transcription factor NF-ĸB p65 in astrocytes but not in microglia. Transmission immunoelectron microscopy data illustrated that HMGB1 was incorporated into sEVs through endosomal mechanisms. In conclusion, proinflammatory mediators released within sEVs can induce cell-specific inflammatory signaling in the brain without activating transmembrane receptors on other cells and causing overt inflammation.

Wearable technologies represent a significant advancement in facilitating communication between humans and machines. Powered by artificial intelligence (AI), human gestures detected by wearable sensors can provide people with seamless interaction with physical, digital, and mixed environments. In this paper, the foundations of a gesture-recognition framework for the teleoperation of infrared consumer electronics are established. This framework is based on force myography data of the upper forearm, acquired from a prototype novel soft pressure-based force myography (pFMG) armband. Here, the sub-processes of the framework are detailed, including the acquisition of infrared and force myography data; pre-processing; feature construction/selection; classifier selection; post-processing; and interfacing/actuation. The gesture recognition system is evaluated using 12 subjects’ force myography data obtained whilst performing five classes of gestures. Our results demonstrate an inter-session and inter-trial gesture average recognition accuracy of approximately 92.2% and 88.9%, respectively. The gesture recognition framework was successfully able to teleoperate several infrared consumer electronics as a wearable, safe and affordable human–machine interface system. The contribution of this study centres around proposing and demonstrating a user-centred design methodology to allow direct human–machine interaction and interface for applications where humans and devices are in the same loop or coexist, as typified between users and infrared-communicating devices in this study.

Immersive Virtual Environments (IVEs), particularly Mixed-Reality (MR) technologies, continue to have an increasing impact on design processes and design education. This study focuses on MR's potentials in Basic Design (BD) education and its influence on novice designers' design thinking abilities. In this study, through a newly developed MR-based design framework, DesignMR, a comparative analysis between design processes in MR and the physical environment is presented. A hybrid evaluation methodology is used in the context of three-dimensional (3D) BD tasks through two sets of protocol studies: Linkography and exit interviews. Linkography is a method to analyze the design process based on detecting design moves and their links with each other. In this study, linkographic analyses point to an increase in critical moves and link index values in MR as compared to the physical environment for all participants. This indicates that MR can trigger improved creativity, design productivity, and design exploration by idea generation. Exit interviews highlighted the positive impact of DesignMR on the motivation, work efficiency, and 3D perception of the participants. Further research will be pursued for improved hand gestures to increase the effectiveness of object interaction.

Abstract Introduction Invasive cervical cancer (ICC) is an HIV-associated cancer that is preventable and precancerous stages including early ICC stages could be detected through screening offering opportunities for treatment and cure. The high incidence in women living with HIV and late presentation often at advanced stages of ICC with limited treatment facilities often result in early mortality. We sought to compare the epidemiologic characteristics and survival differences in HIV status of ICC patients in Nigeria. Methods We conducted a cohort study at two federal academic hospital-based research sites in Jos University Teaching Hospital, and Lagos University Teaching Hospital Nigeria, between March 2018 and September 2022. We enrolled women with histologically confirmed ICC with known HIV status, and FIGO staging as part of the United States of America’s National Institutes of Health/National Cancer Institute funded project titled ‘Epigenomic Biomarkers of HIV-Associated Cancers in Nigeria’. The primary outcome was all-cause mortality with assessment of overall survival (OS) and time to death after ICC diagnosis. OS distribution was estimated using the method of Kaplan–Meier and compared between groups using the log-rank test. Results A total of 239 women with confirmed ICC were enrolled and included in this analysis, of whom 192 (80.3%) were HIV-negative (HIV−/ICC +), and 47 (19.7%) were HIV-positive (HIV +/ICC +). The HIV +/ICC + patients were younger with median age 46 (IQR: 40–51) years compared to 57 (IQR: 45–66) among HIV−/ICC + (P

5,6-dimethylxanthenone-4-acetic acid (DMXAA) is a mouse-selective stimulator of interferon gene (STING) agonist exerting STING-dependent anti-tumor activity. Although DMXAA cannot fully activate human STING, DMXAA reached phase III in lung cancer clinical trials. How DMXAA is effective against human lung cancer is completely unknown. Here, we show that DMXAA is a partial STING agonist interfering with agonistic STING activation, which may explain its partial anti-tumor effect observed in humans, as STING was reported to be pro-tumorigenic for lung cancer cells with low antigenicity. Furthermore, we developed a DMXAA derivative—3-hydroxy-5-(4-hydroxybenzyl)-4-methyl-9H-xanthen-9-one (HHMX)—that can potently antagonize STING-mediated immune responses both in humans and mice. Notably, HHMX suppressed aberrant responses induced by STING gain-of-function mutations causing STING-associated vasculopathy with onset in infancy (SAVI) in in vitro experiments. Furthermore, HHMX treatment suppressed aberrant STING pathway activity in peripheral blood mononuclear cells from SAVI patients. Lastly, HHMX showed a potent therapeutic effect in SAVI mouse model by mitigating disease progression. Thus, HHMX offers therapeutic potential for STING-associated autoinflammatory diseases.