2,445 results on '"Gupta, Sudhir"'
Search Results
2. Fatality due to unusual femoral vein rupture in an injectable drug user
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Yadav, Abhishek, Aravindan, V, Abilash, S, and Gupta, Sudhir K
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- 2021
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3. Scientific interpretation of unusual injuries observed in a death caused by firing from an unlicensed rifled firearm
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Raj, K Karthi Vignesh, Chandran, A. Varun, Gupta, Sudhir K., Yadav, Abhishek, and Ali, Zahid
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- 2022
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4. Long-Term Safety of Facilitated Subcutaneous Immunoglobulin 10% Treatment in US Clinical Practice in Patients with Primary Immunodeficiency Diseases: Results from a Post-Authorization Safety Study.
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Rubinstein, Arye, Mabudian, Mohsen, McNeil, Donald, Patel, Niraj, Wasserman, Richard, Gupta, Sudhir, Carrasco, Paz, Chen, Jie, Garcia, Enrique, Nagy, Andras, and Yel, Leman
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Immunogenicity ,Immunoglobulin replacement ,Inborn errors of immunity ,Quality of life ,Tolerability ,Humans ,Male ,Female ,United States ,Adult ,Adolescent ,Prospective Studies ,Hyaluronoglucosaminidase ,Primary Immunodeficiency Diseases ,Middle Aged ,Infusions ,Subcutaneous ,Child ,Young Adult ,Immunoglobulins ,Injections ,Subcutaneous ,Treatment Outcome ,Aged ,Child ,Preschool ,Immunologic Deficiency Syndromes - Abstract
Facilitated subcutaneous immunoglobulin (fSCIG) 10% is an immunoglobulin replacement therapy that utilizes recombinant human hyaluronidase (rHuPH20) to enhance immunoglobulin dispersion and absorption, allowing for longer treatment intervals similar to intravenous immunoglobulin (up to once monthly). fSCIG 10% is indicated in the USA for treating adults and children aged ≥ 2 years with primary immunodeficiency diseases (PIDs). This prospective, non-interventional, open-label, multicenter, post-authorization safety study (NCT02593188) was conducted in the USA from November 2015 to October 2021 to assess the long-term safety of fSCIG 10% in routine clinical practice. Patients with PIDs aged ≥ 16 years who were prescribed and/or had started fSCIG 10% treatment were enrolled. In total, 253 patients were enrolled and included (full analysis set). Participants received fSCIG 10% treatment for a median (interquartile range) of 10.0 (3.5-11.8) months, with the majority of infusions administered every 4 weeks (54.4% [1197/2201 infusions]) and at home (62.6% [1395/2230 infusions]). Overall, 98.5% of infusions were administered without rate reduction, interruption, or discontinuation due to adverse events (AEs). Treatment-related, non-serious AEs were experienced by 52 patients (20.6%, 284 events). Two patients (0.8%) each experienced one treatment-related serious AE (aseptic meningitis and deep vein thrombosis). Development of antibodies against rHuPH20 was uncommon; 14/196 patients (7.1%) tested positive for binding antibodies (titer ≥ 1:160) with no neutralizing antibodies detected. There was no relationship between anti-rHuPH20 antibody positivity and the occurrence of treatment-related serious or non-serious AEs. Long-term, repeated self-administration of fSCIG 10% was well tolerated in US clinical practice by patients with PIDs.
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- 2024
5. Assess the Living Experiences of Men Suffering from Prostate Cancer in Selected Hospital of North India: A Qualitative Study
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Gupta, Sudhir, Srinivasan, P., and Bankim, Sanasam
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- 2019
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6. Adaptive Cellular Responses following SARS-CoV-2 Vaccination in Primary Antibody Deficiency Patients.
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Gupta, Sudhir, Su, Houfen, Agrawal, Sudhanshu, Demirdag, Yesim, Tran, Michelle, and Gollapudi, Sastry
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Breg ,CD4 Treg ,CD8 Treg ,COVID-19 vaccine ,CVID ,Cytotoxic T cells ,IFNγ ,TFR - Abstract
Since the start of the COVID-19 pandemic, in a short span of 3 years, vaccination against SARS-CoV-2 has resulted in the end of the pandemic. Patients with inborn errors of immunity (IEI) are at an increased risk for SARS-CoV-2 infection; however, serious illnesses and mortality, especially in primary antibody deficiencies (PADs), have been lower than expected and lower than other high-risk groups. This suggests that PAD patients may mount a reasonable effective response to the SARS-CoV-2 vaccine. Several studies have been published regarding antibody responses, with contradictory reports. The current study is, perhaps, the most comprehensive study of phenotypically defined various lymphocyte populations in PAD patients following the SARS-CoV-2 vaccine. In this study, we examined, following two vaccinations and, in a few cases, prior to and following the 1st and 2nd vaccinations, subsets of CD4 and CD8 T cells (Naïve, TCM, TEM, TEMRA), T follicular helper cells (TFH1, TFH2, TFH17, TFH1/17), B cells (naïve, transitional, marginal zone, germinal center, IgM memory, switched memory, plasmablasts, CD21low), regulatory lymphocytes (CD4Treg, CD8Treg, TFR, Breg), and SARS-CoV-2-specific activation of CD4 T cells and CD8 T cells (CD69, CD137), SARS-CoV-2 tetramer-positive CD8 T cells, and CD8 CTL. Our data show significant alterations in various B cell subsets including Breg, whereas only a few subsets of various T cells revealed alterations. These data suggest that large proportions of PAD patients may mount significant responses to the vaccine.
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- 2024
7. Concealed Homicides
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Kanwar, Hemant Kumar, DebBarma, Antara, Dey, Arijit, Yadav, Abhishek, and Gupta, Sudhir K
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- 2018
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8. IgD+IgM- B Cells in Common Variable Immunodeficiency.
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Kasahara, Taissa and Gupta, Sudhir
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B cell ,CVID ,IgD ,respiratory allergic diseases ,tolerance - Abstract
Common variable immunodeficiency (CVID) is the most frequent form of primary hypogammaglobulinemia in adults. In addition to recurrent infections and respiratory manifestations, CVID patients may present several non-infection complications such as autoimmune diseases. The mechanisms that lead to immune dysregulation in CVID are not completely understood. Given the role of IgD on naïve B cells in the maintenance of tolerance and secreted IgD in the respiratory mucosa, we evaluated the frequency of IgD+ naïve and IgD+ memory B cells in CVID patients. Here, no differences were observed in the percentages and proliferative responses of anergic IgD+IgM-CD27- B cells between CVID patients, with or without autoimmune disease, and the control group. Interestingly, in the compartment of memory B cells, the percentage of IgD+IgM- cells was higher only in CVID patients with allergic rhinitis/allergic asthma. Our results may indicate that anergic IgD+IgM-CD27- B cells may not be compromised in our CVID cohort. However, IgD+IgM- memory B cells may play a role in the immunopathogenesis of allergic rhinitis/allergic asthma in CVID patients. Further studies are needed to better understand the participation of IgD+IgM- memory B cells in the immunopathogenesis of allergic rhinitis/allergic asthma in CVID patients.
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- 2024
9. Alleged Medical Negligence Cases: An Autopsy Study
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Yadav, Abhishek, Tellewar, Sumit, Swain, Rajanikanta, and Gupta, Sudhir Kumar
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- 2017
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10. An Interesting Case of Accidental Smothering in an Unusual Place
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Yadav, Abhishek, Kumar, Mahesh, Debbarma, Antara, and Gupta, Sudhir Kumar
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- 2018
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11. Extraction and Identification of ‘Finit 'In Biological Samples Using Different Solvent Systems of TLC
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Choudhary, Pallavi, Jaiswal, A.K., Kumar, Adarsh, and Gupta, Sudhir Kumar
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- 2015
12. Higgs Information and NMSSM at the Large Hadron Collider
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Gupta, Surabhi and Gupta, Sudhir Kumar
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High Energy Physics - Phenomenology ,High Energy Physics - Experiment ,High Energy Physics - Theory - Abstract
Information theory has proven to be a worthwhile tool for investigating the implications of the Higgs sector in the Next-to-minimal supersymmetric Standard Model (NMSSM) using Higgs information at the Large Hadron Collider assessed through the entropy constructed by means of the branching ratios of decay channels of the Higgs boson. The present article focuses on the parameter space of supersymmetric extension with an extra term of gauge singlet in light of various experimental constraints. Our findings show the most preferred values of $m_0$, $m_{1/2}$, $ A_0$, $ tan\beta$, $\lambda$, $\mu_{eff}$, neutralino LSP $ m_{\tilde\chi^{0}_{1}}$, lightest chargino $ m_{\tilde\chi^{\pm}_{1}}$, singlino $ m_{\tilde\chi^{0}_{5}}$, and gluino $ m_{\tilde g}$ to be around 1.93 TeV, 1.78 TeV, $-$3.62 TeV, 27.5, 0.012, 665.7 GeV, 0.74 TeV, 0.79 TeV, 11.24 TeV, and 3.70 TeV, respectively, that is compatible with the relic density of dark matter., Comment: 16 pages, 4 figures
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- 2023
13. PMCT-based sex determination using posterior segment of greater sciatic notch in North Indian population
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Kumari, Ruchi, Pandit, Jay Narayan, Panga, Surya Kiran, Tyagi, Swati, Yadav, Abhishek, and Gupta, Sudhir Kumar
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- 2024
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14. Transferability of cowpea and azuki bean derived SSR markers to other Vigna species
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Bansal, Ravindra, Gupta, Sudhir Kumar, and Gopalakrishna, T.
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- 2012
15. Effect of integrated nutrient management on yield, nutrient uptake and changes in soil fertility under sugarcane-ratoon system in sub- humid agro eco-system of rajasthan
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Dashora, Pramod and Gupta, Sudhir
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- 2012
16. New physics contributions to $Wtb$ anomalous couplings and top-quark decay
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Tiwari, Apurba and Gupta, Sudhir Kumar
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High Energy Physics - Phenomenology ,High Energy Physics - Experiment - Abstract
In this work, we study the new physics effects arising due the presence of anomalous $Wtb$ vertex through the semileptonic decay modes of the top-quark at the Large Hadron Collider. An estimate on the sensitivities of the aforementioned interaction at 5$\sigma$ CL in the context of top-quark decay-width measurements and cross-section measurements would also be discussed for the pre-existing 13 TeV LHC data and its projections for the proposed LHC runs at 14 TeV, 27 TeV and 100 TeV. We also incorporate the $\mathcal CP$-violating effects to such interactions by constructing the $\mathcal CP$-violating asymmetries., Comment: 4 pages, 2 figures, 2 tables, To be published in the proceedings of International Conference on High Energy Physics (ICHEP 2022), Bologna (Itly)
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- 2022
17. $\mathcal CP$-violating anomalous interactions at Large Hadron Collider
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Tiwari, Apurba and Gupta, Sudhir Kumar
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High Energy Physics - Phenomenology ,High Energy Physics - Experiment - Abstract
In this study, we explore the effects of $\mathcal CP$-violating anomalous interactions of the top-quark through the semileptonic decay modes of the top-quark arising due to pair-production of $t\bar{t}$ at the Large Hardon Collider. Predictions on the LHC sensitivities of the coupling strength to such $\mathcal CP$-violating interactions would be discussed for the 13 TeV LHC data and for the future hadron collider with 14 TeV energy., Comment: 6 pages, 2 figures, Proceedings of the LHCP 2022 conference
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- 2022
18. Higgs boson in a flavor-extension of the CMSSM
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Gupta, Surabhi, Gupta, Sudhir Kumar, and Ren, Keven
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High Energy Physics - Phenomenology ,High Energy Physics - Experiment - Abstract
Flavour-violating couplings of Higgs boson with stop and scharm quarks could be very important as in addition to lifting the mass of the Higgs boson by a few GeV, it could also play a vital phenomenological role in reducing the Supersymmetry breaking scale significantly. In this work, we investigate effects of such flavour-violating couplings within the Constrained Minimal Supersymmetric Standard Model (CMSSM) framework in the context of LEP data, Higgs data at the LHC, precision observables and the relic density of the dark matter using Bayesian statistics. Our detailed analysis reveals that the most probable values of $m_{0}$, $m_{1/2}$, $A_{0}$, $\tan{\beta}$, $\delta^{LR}_{ct}$ are expected to be around 4.83 TeV, 2.54 TeV, 1.90 TeV, 41.5, and 6.1$\times10^{-2}$, respectively, with flat priors. The corresponding values translate into 3.25 TeV, 2.13 TeV, 1.90 TeV, 44.7, and 5.9$\times10^{-2}$, respectively, if the natural priors are used. Furthermore, a comparison of our model with the CMSSM of flavour-conservation as the base model yields a Bayes factor of about 6 while taking into account all the experimental constraints used in our study. Our analysis also reflects that the lightest neutralino would have a mass of about 1 TeV., Comment: 17 pages, 7 figures
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- 2022
19. $t\bar{t}H$ Interactions and T-odd Correlations at Hadron Colliders
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Tiwari, Apurba and Gupta, Sudhir Kumar
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High Energy Physics - Phenomenology ,High Energy Physics - Experiment - Abstract
We explore $\mathcal CP$-violation effects of Higgs-top interactions in the associated production of a Higgs boson with top-pair in the dileptonic decay modes of top-quark originating from proton proton collisions $pp \to t\bar{t}H \to (l^+ \nu_l b)(l^{-} \bar{\nu_l} \bar{b})H$ at NLO in QCD matched to parton shower via T-odd observables using momenta of various particles involved in the process. In particular, we predict the constraints on the $\mathcal CP$-violating $t\bar{t}H$ coupling obtained through the production asymmetries associated with the T-odd observables in the dileptonic decay channel of the $t\bar{t}$ pair for the LHC with centre-of-mass energy of 13 TeV and an integrated luminosity of 139 fb$^{-1}$. We also present the corresponding limits for future hadron colliders, namely the High Luminosity LHC (HL-LHC) and the Future Circular Collider (FCC-HH). Our estimates of the $t\bar{t}H$ interaction strength reveal that the upper bound on pseudoscalar coupling $c_p$ corresponding to the largest asymmetry would be of about $1.96 \times 10^{-2}$ at 2.5$\sigma$ C.L. for $c_s$ = 1 at the LHC with $\sqrt{S}$ = 13 TeV for an integrated luminosity of 139 fb$^{-1}$. The respective limits for HL-LHC and FCC-hh with the projected Luminosities of 3 ab$^{-1}$ and 30 ab$^{-1}$ are found to be to $3.4\times 10^{-3}$ and $1.6\times 10^{-4}$ respectively at 2.5$\sigma$ C.L., Comment: 31 pages, 8 figures, 6 Tables
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- 2022
20. Higgs information in Split-SUSY at the LHC
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Gupta, Surabhi and Gupta, Sudhir Kumar
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High Energy Physics - Phenomenology ,High Energy Physics - Experiment - Abstract
Information theory turns out to be an interesting tool for studying the consequences of Higgs observations to various new physics candidate theories by means of the information measure as the entropy of Higgs-Boson through its various detection modes at the Large Hadron Collider. The present article investigates the parameter space of a supersymmetric scenario where sfermions and one of the Higgs superfields are decoupled, while the gauginos, Higgsinos, and the remaining Higgs doublet are still allowed to be lighter. Our analysis reveals that this is quite a viable choice in the light of LHC discovery of a Higgs which resembles the SM Higgs-Boson and nothing else so far. While the supersymmetry breaking scale $M_S$ could be as high as $10^{11}$ GeV or so, the most preferred values of the $M_{S}$ and $\tan\beta$ are found to be around 3.6$\times10^7$ GeV and $41$ respectively, which is also consistent with the relic abundance of the neutralino dark matter. The corresponding value of neutralino ($ m_{\tilde\chi^{0}_{1}}$) LSP is estimated to be around 1.01 TeV. The preferred values of other parameters, namely, the Higgsino mass ($\mu$) and gaugino mass parameters ($M_1$ and $M_2$) are found to be about 1.05 TeV, 1.74 TeV, and 2.57 TeV, respectively., Comment: 13 pages, 3 figures, 3 tables
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- 2022
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21. Leptin favors imbalance of antigen-specific CD4+ T-cells associated with severity of cat allergy.
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Vollmer, Carolina, Dias, Aleida, Sales, Marisa, Sacramento, Priscila, Silva, Júlio, Oyamada, Hugo, Linhares, Ulisses, Gupta, Sudhir, Kasahara, Taissa, and Bento, Cleonice
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Fel d1 ,Tfh cells ,Tfr cells ,Th2/Th9 ,Treg/Tr-1 cells ,leptin ,Humans ,CD4-Positive T-Lymphocytes ,Cytokines ,Hypersensitivity ,Immunoglobulin E ,Interleukin-13 ,Interleukin-4 ,Interleukin-5 ,Leptin ,Leukocytes ,Mononuclear ,Obesity - Abstract
INTRODUCTION: Obesity can complicate IgE-mediated allergic diseases. In the present study, we aimed to investigate the ability of obesity-related concentrations of leptin to modulate the in vitro effector and regulatory Fel d1-specific CD4+ T-cell subsets in patients allergic to cat, considered the third most common cause of respiratory allergy in humans. METHODS: For this study, plasma and peripheral blood mononuclear cells (PBMC) from 30 cat-allergic patients with mild, moderate and severe respiratory symptoms were obtained. The PBMC cultures were stimulated with Fel d1 antigen (10 µg/mL) in the presence or absence of obesity-related leptin dose (50 ηg/mL). After 6 days, the levels of cytokines and IgE in the supernatants were evaluated by multiplex and ELISA, respectively. The frequency of different non-follicular (CXCR5-) and follicular (CXCR5+) Fel d1-specific CD4+ T cell subsets was determined by flow cytometry. The plasma levels of leptin and IgE anti-cat titers were evaluated by ELISA and ImmunoCAP, respectively. RESULTS AND CONCLUSIONS: Fel d1 induced both IgE production and release of cytokines related to Th2, Th9 and Th17 cell phenotypes. Feld1 was more efficient in increasing the frequency of TFHIL-21- cells positive for IL-4, IL-5 and IL-13 than TFHIL-21+ cell subsets. Leptin favored the expansion Th2-like and Th9-like cells and TFHIL-21- cells positive for IL-4, IL-5 and IL-13, but reduced the proportion of conventional (Treg/Tr-1) and follicular (TFR) regulatory CD4+ T-cell subsets expressing or not CD39 marker. Finally, many of the imbalances between Fel d1-specific CD4+ T-cells were also correlated with plasma leptin and anti-Fel d1 IgE titers. In summary, hyperleptinemia should negatively impact on the severity of cat allergies by favoring the expansion of pathogenic Fel d1-specific CD4+ T-cell phenotypes and damaging the functional status of regulatory CD4+ T-cell subsets.
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- 2023
22. Dendritic cells in inborn errors of immunity.
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Gupta, Sudhir and Agrawal, Anshu
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Dendritic Cells ,Humans ,Inflammation ,Autoimmunity ,Drug Tolerance ,Primary Immunodeficiency Diseases ,classical DCs ,dendritic cells ,inborn errors of immunity ,monocyte derived DCs ,myeloid DCs ,plasmacytoid DCs ,primary immunodeficiencies ,Vaccine Related ,Autoimmune Disease ,Genetics ,Prevention ,Biodefense ,Pediatric ,Inflammatory and immune system ,Immunology ,Medical Microbiology - Abstract
Dendritic cells (DCs) are crucial cells for initiating and maintaining immune response. They play critical role in homeostasis, inflammation, and autoimmunity. A number of molecules regulate their functions including synapse formation, migration, immunity, and induction of tolerance. A number of IEI are characterized by mutations in genes encoding several of these molecules resulting in immunodeficiency, inflammation, and autoimmunity in IEI. Currently, there are 465 Inborn errors of immunity (IEI) that have been grouped in 10 different categories. However, comprehensive studies of DCs have been reported in only few IEI. Here we have reviewed biology of DCs in IEI classified according to recently published IUIS classification. We have reviewed DCs in selected IEI in each group category and discussed in depth changes in DCs where significant data are available regarding role of DCs in clinical and immunological manifestations. These include severe immunodeficiency diseases, antibody deficiencies, combined immunodeficiency with associated and syndromic features, especially disorders of synapse formation, and disorders of immune regulation.
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- 2023
23. Investigating non-minimal flavour-violating CMSSM in the light of Higgs-Boson mass using information theory
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Gupta, Surabhi and Gupta, Sudhir Kumar
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High Energy Physics - Phenomenology ,High Energy Physics - Experiment ,High Energy Physics - Theory - Abstract
Flavour-violating interactions of the stop-quarks are expected to provide an additional few GeV contributions to the Higgs-Boson mass, particularly when mix with scharm-quarks, thereby allowing reduced supersymmetry (SUSY) breaking scale compared to flavour-conserving constrained minimal supersymmetric Standard Model (CMSSM). Inspired by this, we analyse the interactions mentioned above in the context of CMSSM using the information entropy of the Higgs-Boson for a wider region of flavour-violating CMSSM parameter space $(m_0,m_{1/2},A_0,$ $\tan\beta,sgn(\mu),\delta_{ct}^{ij})$, where $\delta_{ct}^{ij}$ represents the flavour-violating coupling of the top-quark with the charm-quark and $i, j$ defining left and right chiralities of squarks. Our information-theoretic analysis of the model mentioned above reveals the most favourable values of $(m_0, m_{1/2},A_0, \tan\beta,\delta_{ct}^{ij})$ as $(4.30 {\rm~TeV}, 2.32 {\rm~TeV}, -4.96{\rm~ TeV},$ $ 22.8,0.037)$ and $(4.16 {\rm ~TeV},$ $3.89~{\rm TeV},-4.10~{\rm TeV}, 19.4, 0.039)$ for $(i, j) = (L, R)$ and $(R, L)$, respectively, corresponding to the maximum entropy which suggest the SUSY breaking scale to be about $5$ TeV, thereby allowing considerable low values of sparticles masses than the flavour-conserving CMSSM., Comment: 17 pages, 8 figures
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- 2022
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24. The anomalous $Wtb$ vertex and top-pair production at the LHC
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Tiwari, Apurba and Gupta, Sudhir Kumar
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High Energy Physics - Phenomenology - Abstract
We perform an exclusive study of the anomalous $Wtb$ interaction in the context of LHC. The limits on $Wtb$ anomalous couplings have been estimated via the measurements of the top-quark decay width and cross-section as well as production asymmetries in case the CP is violated. Our investigations reveal that the upper bounds on $(|C_L|, |C_R|)$ would be of about (1.82, 0.03) $\times 10^{-4}$ at $2.5 \sigma$ C.L., for the already collected data at the LHC with $\sqrt{S} = 13$ TeV for an integrated luminosity of 139 fb$^{-1}$. The corresponding limits for future hadron colliders, namely, High Luminosity LHC (HL-LHC), High Energy LHC (HE-LHC) and Future Circular Collider (FCC-hh) for the projected luminosities of 3 ab$^{-1}$, 12 ab$^{-1}$ and 30 ab$^{-1}$ are found to be to $(0.81, 0.006)\times 10^{-4}$, $(0.44, 0.0017)\times 10^{-4}$ and $(0.21, 0.0004)\times 10^{-4}$ respectively at 2.5$\sigma$ C. L.., Comment: 18 pages, 11 figures
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- 2022
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25. Potential mechanisms of death in cases of fatal acid attacks
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Byard, Roger W., Behera, Chittaranjan, Gupta, Sudhir K., Chauhan, Mohit, and Kaur, Navpreet
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- 2023
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26. Quantitative analysis of methanol in blood, urine, vitreous humor, and cerebrospinal fluid by using gas chromatography-head space in two suspected cases of methanol poisoning
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Afaria, Neha, Jaiswal, A. K., Janarthanan, Venkatesh, Gupta, Sudhir Kumar, Millo, T., and Gokul, G.
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- 2023
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27. The Impact of Long–Term Orientation Traits on Pandemic Fatigue Behavior: Evidence from the Columbian Exchange
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Roy, Sutanuka, Gupta, Sudhir, and Tourky, Rabee
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- 2023
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28. Subcutaneous Immunoglobulin 16.5% (Cutaquig®) in Primary Immunodeficiency Disease: Safety, Tolerability, Efficacy, and Patient Experience with Enhanced Infusion Regimens
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Gupta, Sudhir, DeAngelo, James, Melamed, Isaac, Walter, Jolan E., Kobayashi, Ai-Lan, Bridges, Tracy, Sublett, J. Wesley, Bernstein, Jonathan A., Koterba, Alan, Manning, Michael, Maltese, Joanna, Hoeller, Sonja, Turpel-Kantor, Eva, Kreuwel, Huub, and Kobayashi, Roger H.
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- 2023
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29. SARS-CoV-2-Specific and Functional Cytotoxic CD8 Cells in Primary Antibody Deficiency: Natural Infection and Response to Vaccine
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Gupta, Sudhir, Agrawal, Sudhanshu, Sandoval, Ashley, Su, Houfen, Tran, Michelle, and Demirdag, Yesim
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Biomedical and Clinical Sciences ,Immunology ,Vaccine Related ,Emerging Infectious Diseases ,Biodefense ,Lung ,Clinical Research ,Pneumonia ,Prevention ,Immunization ,Infectious Diseases ,Infection ,Good Health and Well Being ,Antibodies ,Viral ,BNT162 Vaccine ,CD8-Positive T-Lymphocytes ,COVID-19 ,COVID-19 Vaccines ,Humans ,Immunologic Memory ,Primary Immunodeficiency Diseases ,SARS-CoV-2 ,Spike Glycoprotein ,Coronavirus ,CTLs ,SARS-CoV-2-specific CD8 T cells ,XLA ,CVID ,Pfizer/BNT vaccine - Abstract
PurposeCD8 cytotoxic T cells (CTLs) play a critical role in the clearance of virally infected cells. SARS-CoV-2-specific CD8 T cells and functional CTLs in natural infections and following COVID-19 vaccine in primary antibody deficiency (PAD) have not been reported. In this study, we evaluated T cell response following COVID-19 or COVID-19 mRNA vaccination in patients with PADs by assessing SARS-CoV-2 tetramer-positive CD8 T cells and functional CTLs.MethodsSARS-CoV-2-specific CD8 and functional CTLs were examined in a patient with X-linked agammaglobulinemia (XLA) and a patient with common variable immunodeficiency (CVID) following COVID-19 infection, and in 5 patients with CVID and 5 healthy controls 1 month following 2nd dose of COVID-19 mRNA vaccine (Pfizer-BioNTech). Cells were stained with SARS-CoV-2 spike protein-specific tetramers, and for functional CTLs (CD8+ CD107a+ granzyme B+ perforin+), with monoclonal antibodies and isotype controls and analyzed by flow cytometry.ResultsSARS-CoV-2-specific tetramer + CD8 T cells and functional CTLs in the patient with XLA following COVID-19 infection were higher, as compared to healthy control subject following COVID-19 infection. On the other hand, SARS-CoV2-tetramer + CD8 T cells and functional CTLs were lower in CVID patient following COVID19 infection as compared to healthy control following COVID-19 infection. SARS-CoV2-tetramer + CD8 T cells and functional CTLs were significantly lower in SARS-CoV2-naive CVID patients (n = 10) following vaccination when compared to SARS-CoV-2-naive healthy vaccinated controls (n = 10).ConclusionsCVID is associated with reduced SARS-CoV-2-specific CD8 T cells and functional CTLs in both natural SARS-CoV-2 infection and in response to SARS-CoV-2 mRNA vaccine, whereas natural infection in XLA is associated with a robust SARS-CoV-2-specific CD8 and functional CTL responses.
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- 2022
30. Leptin favors Th17/Treg cell subsets imbalance associated with allergic asthma severity
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Vollmer, Carolina M, Dias, Aleida SO, Lopes, Lana M, Kasahara, Taissa M, Delphim, Letícia, Silva, Júlio Cesar C, Lourenço, Lucas Paulo, Gonçalves, Hilary Cesário, Linhares, Ulisses C, Gupta, Sudhir, and Bento, Cleonice AM
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Nutrition ,Lung ,Clinical Research ,Obesity ,2.1 Biological and endogenous factors ,Aetiology ,Inflammatory and immune system ,allergic asthma ,leptin ,obesity ,regulatory T cells ,Th17 cells - Abstract
BackgroundObesity has often been associated with severe allergic asthma (AA). Here, we analyzed the frequency of different circulating CD4+T-cell subsets from lean, overweight and obese AA patients.MethodsMononuclear cells from peripheral blood were obtained from 60 AA patients and the frequency of different CD4+T-cell subsets and type 1 regulatory B cells (Br1) was determined by cytometry. The effect of obese-related leptin dose on cytokine production and Treg cell function in AA-derived CD4+ T cell cultures was evaluated by ELISA and 3H thymidine uptake, respectively. Leptin levels were quantified in the plasma by ELISA. According to the BMI, patients were stratified as lean, overweight and obese.ResultsAA severity, mainly among obese patients, was associated with an expansion of hybrid Th2/Th17 and Th17-like cells rather than classic Th2-like cells. On the other hand, the frequencies of Th1-like, Br1 cells and regulatory CD4+ T-cell subsets were lower in patients with severe AA. While percentages of the hybrid Th2/Th17 phenotype and Th17-like cells positively correlated with leptin levels, the frequencies of regulatory CD4+ T-cell subsets and Br1 cells negatively correlated with this adipokine. Interestingly, the obesity-related leptin dose not only elevated Th2 and Th17 cytokine levels, but also directly reduced the Treg function in CD4+ T cell cultures from lean AA patients.ConclusionIn summary, our results indicated that obesity might increase AA severity by favoring the expansion of Th17-like and Th2/Th17 cells and decreasing regulatory CD4+T cell subsets, being adverse effects probably mediated by leptin overproduction.
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- 2022
31. Comparative study of postmortem computed tomography (PMCT) against traditional forensic autopsy findings in fatal road traffic accidents — a pilot analysis
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K., Karthi Vignesh Raj, Khan, Anam, Yadav, Abhishek, Jana, Manisha, Gupta, Sudhir K., A., Varun Chandran, D., Balaji, Simon, Alex Eldo, and Chauhan, Aditya Pratap Singh
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- 2023
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32. Corrigendum: Clinical efficacy, safety and tolerability of a new subcutaneous immunoglobulin 16.5% (Octanorm [Cutaquig®]) in the treatment of patients with primary immunodeficiencies
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Kobayashi, Roger H, Gupta, Sudhir, Melamed, Isaac, Mandujano, J Fernando, Kobayashi, Ai Lan, Ritchie, Bruce, Geng, Bob, Atkinson, Thomas Prescott, Rehman, Syed, Turpel-Kantor, Eva, and Litzman, Jiří
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Biomedical and Clinical Sciences ,Clinical Sciences ,Good Health and Well Being ,primary immunodeficiencies ,immunoglobulins ,antibodies ,SCIG ,infections ,infusion site reactions ,Immunology ,Medical Microbiology ,Biochemistry and cell biology ,Genetics - Abstract
[This corrects the article DOI: 10.3389/fimmu.2019.00040.].
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- 2022
33. Members of the Regulatory Lymphocyte Club in Common Variable Immunodeficiency
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Gupta, Sudhir, Demirdag, Yesim, and Gupta, Ankmalika Abha
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Biomedical and Clinical Sciences ,Immunology ,Autoimmune Disease ,Clinical Research ,2.1 Biological and endogenous factors ,Aetiology ,Inflammatory and immune system ,Animals ,Autoimmune Diseases ,Autoimmunity ,Common Variable Immunodeficiency ,Humans ,Mice ,T-Lymphocytes ,Regulatory ,Breg ,CD8 Treg ,CVID ,T follicular regulatory cells ,Treg ,autoimmunity ,germinal center ,Medical Microbiology ,Biochemistry and cell biology ,Genetics - Abstract
The role of CD4 T regulatory cells is well established in peripheral tolerance and the pathogenesis of the murine model and human autoimmune diseases. CD4 T regulatory cells (CD4 Tregs) have been investigated in common variable immunodeficiency (CVID). Recently, additional members have been added to the club of regulatory lymphocytes. These include CD8 T regulatory (CD8 Tregs), B regulatory (Bregs), and T follicular helper regulatory (TFR) cells. There are accumulating data to suggest their roles in both human and experimental models of autoimmune disease. Their phenotypic characterization and mechanisms of immunoregulation are evolving. Patients with CVID may present or are associated with an increased frequency of autoimmunity and autoimmune diseases. In this review, we have primarily focused on the characteristics of CD4 Tregs and new players of the regulatory club and their changes in patients with CVID in relation to autoimmunity and emphasized the complexity of interplay among various regulatory lymphocytes. We suggest future careful investigations of phenotypic and functional regulatory lymphocytes in a large cohort of phenotypic and genotypically defined CVID patients to define their role in the pathogenesis of CVID and autoimmunity associated with CVID.
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- 2022
34. Immune Response to SARS-CoV-2 Vaccine in 2 Men
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Gupta, Sudhir, Su, Houfen, and Agrawal, Sudhanshu
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Biomedical and Clinical Sciences ,Immunology ,Prevention ,Clinical Research ,Pneumonia & Influenza ,Vaccine Related ,Infectious Diseases ,Immunization ,Biotechnology ,Emerging Infectious Diseases ,HIV/AIDS ,Biodefense ,Inflammatory and immune system ,Good Health and Well Being ,Aged ,Antibodies ,Viral ,B-Lymphocytes ,BNT162 Vaccine ,CD4-Positive T-Lymphocytes ,CD8-Positive T-Lymphocytes ,COVID-19 ,Humans ,Immunoglobulin G ,Male ,Middle Aged ,SARS-CoV-2 ,T-Lymphocytes ,Regulatory ,Vaccination ,Memory T cells ,Memory B cells ,Regulatory lymphocytes ,Follicular helper T cells ,SARS-CoV-2-specific CD8 T cells ,Vaccine ,Allergy - Abstract
IntroductionIn the trials of corona virus vaccines, detailed analyses of subsets of lymphocytes were not carried out. We present perhaps the most comprehensive immunological analysis of 29 subsets of B and T cells in 2 healthy subjects receiving 2 doses of the Pfizer SARS-CoV-2 (COVID-19) vaccine.MethodsAnalyses were performed prior to vaccination, 3 weeks following the 1st dose, and 4 weeks following the 2nd dose. Total, naïve (TN), and different memory and effector subsets (TCM, TEM, and TEMRA) of CD4+ and CD8+ T cells; SARS-CoV-2 spike protein-specific tetramer+, and cytotoxic CD8+ T; subsets of T follicular cells (TFH, TFH1, TFH2, TFH1/TFH17, and TFH17); B-cell subsets (mature B cells, naive B cells, transitional B cells, marginal zone B cells, class-switched memory B cells, germinal center B cells, and CD21low B cells), and plasmablasts; and regulatory lymphocytes (CD4+ Treg, CD8+ Treg, Breg, and TFR cells) were evaluated with specific monoclonal antibodies by flow cytometry.ResultsA lack of COVID-19 IgG antibodies after the 1st dose in one of 2 subjects was associated with increased regulatory lymphocytes and decreased plasmablasts. Seroconversion after the 2nd dose in this subject was associated with decreased TFR cells and increased plasmablasts. In both subjects, CD4 TEM and CD8 TCM were markedly increased following the 2nd dose. TFH1 and regulatory lymphocytes were increased (except Breg) following the 1st dose. A striking increase in SARS-CoV-2-specific CD8+ T cells was observed following the 2nd dose.ConclusionOur data support the need for 2nd dose of vaccine to induce strong SARS-CoV-2 CD8 T-cell specific response and generation of memory subsets of CD4+ and CD8+ T cells. Regulatory lymphocytes appear to play a role in the magnitude of response.
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- 2022
35. Interindividual immunogenic variants: Susceptibility to coronavirus, respiratory syncytial virus and influenza virus
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Darbeheshti, Farzaneh, Mahdiannasser, Mojdeh, Uhal, Bruce D, Ogino, Shuji, Gupta, Sudhir, and Rezaei, Nima
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Microbiology ,Biological Sciences ,Genetics ,Prevention ,Influenza ,Immunization ,Vaccine Related ,Lung ,Pneumonia & Influenza ,Infectious Diseases ,Emerging Infectious Diseases ,Biodefense ,Aetiology ,2.1 Biological and endogenous factors ,Infection ,Inflammatory and immune system ,Good Health and Well Being ,Antiviral Agents ,Biological Variation ,Individual ,COVID-19 ,Cytokine Release Syndrome ,Gene Expression ,Genetic Predisposition to Disease ,Humans ,Immunity ,Innate ,Immunologic Factors ,Influenza ,Human ,Mannose-Binding Lectin ,Orthomyxoviridae ,Respiratory Syncytial Virus Infections ,Respiratory Syncytial Viruses ,Severe acute respiratory syndrome-related coronavirus ,SARS-CoV-2 ,Severe Acute Respiratory Syndrome ,Toll-Like Receptors ,COVID-19 Drug Treatment ,Covid-19 ,cytokine storm ,genetic susceptibility ,influenza virus ,immune-related variants ,RSV ,Medical Microbiology ,Virology - Abstract
The coronavirus disease (Covid-19) pandemic is the most serious event of the year 2020, causing considerable global morbidity and mortality. The goal of this review is to provide a comprehensive summary of reported associations between inter-individual immunogenic variants and disease susceptibility or symptoms caused by the coronavirus strains severe acute respiratory syndrome-associated coronavirus, severe acute respiratory syndrome-associated coronavirus-2, and two of the main respiratory viruses, respiratory syncytial virus and influenza virus. The results suggest that the genetic background of the host could affect the levels of proinflammatory and anti-inflammatory cytokines and might modulate the progression of Covid-19 in affected patients. Notably, genetic variations in innate immune components such as toll-like receptors and mannose-binding lectin 2 play critical roles in the ability of the immune system to recognize coronavirus and initiate an early immune response to clear the virus and prevent the development of severe symptoms. This review provides promising clues related to the potential benefits of using immunotherapy and immune modulation for respiratory infectious disease treatment in a personalized manner.
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- 2021
36. Cancer: A Complex Problem Requiring Interdisciplinary Research
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Yazdanpanah, Niloufar, Dochy, Filip, Darmstadt, Gary L., Peters, Godefridus J., Tsitlakidis, Abraham, Aifantis, Elias C., Cerda, Artemi, Comini, Elisabetta, Brand, Serge, Gupta, Manoj, Cheson, Bruce D., Thomas, Sabu, Tanzer, Michael, Weiskirchen, Ralf, Bella, Federico, Fereshtehnejad, Seyed-Mohammad, Nikita, Konstantina, Ali, Imran, Kato, Koichi, Poggi, Alessandro, Jon, Ernest Chua Kian, Rao, Idupulapati M., Tao, Xiaoming, He, Ji-Huan, Rao, Lingamallu Jagan Mohan, Leemans, Alexander, Pomponio, Alessio, Hernandez, Alfredo Martínez, Ahmadieh, Hamid, Sahraian, Mohammad Ali, Kelishadi, Roya, Thongboonkerd, Visith, Bahinipati, Seema, Toi, Masakazu, von Herrath, Matthias, Sellke, Frank, Sherwood, Steven, Perry, George, Nieto, Juan J., Gupta, Sudhir, Dorigo, Tommaso, Mobasher, Bahram, Ochs, Hans D., Rezaei, Nima, Rezaei, Nima, Series Editor, Ahmed, Atif A., Editorial Board Member, Aguiar, Rodrigo, Editorial Board Member, Ambrosio, Maria R., Editorial Board Member, Artac, Mehmet, Editorial Board Member, Augustine, Tanya N., Editorial Board Member, Bambauer, Rolf, Editorial Board Member, Bhat, Ajaz Ahmad, Editorial Board Member, Bertolaccini, Luca, Editorial Board Member, Bianchini, Chiara, Editorial Board Member, Cavic, Milena, Editorial Board Member, Chakrabarti, Sakti, Editorial Board Member, Cho, William C. S., Editorial Board Member, Czarnecka, Anna M., Editorial Board Member, Domingues, Cátia, Editorial Board Member, Eşkazan, A. Emre, Editorial Board Member, Fares, Jawad, Editorial Board Member, Fonseca Alves, Carlos E., Editorial Board Member, Fru, Pascaline, Editorial Board Member, Da Gama Duarte, Jessica, Editorial Board Member, García, Mónica C., Editorial Board Member, Gener, Melissa A.H., Editorial Board Member, Estrada Guadarrama, José Antonio, Editorial Board Member, Hargadon, Kristian M., Editorial Board Member, Holvoet, Paul, Editorial Board Member, Jurisic, Vladimir, Editorial Board Member, Kabir, Yearul, Editorial Board Member, Katsila, Theodora, Editorial Board Member, Kleeff, Jorg, Editorial Board Member, Liang, Chao, Editorial Board Member, Tan, Mei Lan, Editorial Board Member, Li, Weijie, Editorial Board Member, Prado López, Sonia, Editorial Board Member, Macha, Muzafar A., Editorial Board Member, Malara, Natalia, Editorial Board Member, Orhan, Adile, Editorial Board Member, Prado-Garcia, Heriberto, Editorial Board Member, Pérez-Velázquez, Judith, Editorial Board Member, Rashed, Wafaa M., Editorial Board Member, Sanguedolce, Francesca, Editorial Board Member, Sorrentino, Rosalinda, Editorial Board Member, Shubina, Irina Zh., Editorial Board Member, de Araujo, Heloisa Sobreiro Selistre, Editorial Board Member, Torres-Suárez, Ana Isabel, Editorial Board Member, Włodarczyk, Jakub, Editorial Board Member, Yeong, Joe Poh Sheng, Editorial Board Member, Toscano, Marta A., Editorial Board Member, Wong, Tak-Wah, Editorial Board Member, Yin, Jun, Editorial Board Member, and Yu, Bin, Editorial Board Member
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- 2023
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37. Malignancies in Inborn Errors of Immunity
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Demirdag, Yesim Yilmaz, Gupta, Sudhir, Rezaei, Nima, Series Editor, Ahmed, Atif A., Editorial Board Member, Aguiar, Rodrigo, Editorial Board Member, Ambrosio, Maria R., Editorial Board Member, Artac, Mehmet, Editorial Board Member, Augustine, Tanya N., Editorial Board Member, Bambauer, Rolf, Editorial Board Member, Bhat, Ajaz Ahmad, Editorial Board Member, Bertolaccini, Luca, Editorial Board Member, Bianchini, Chiara, Editorial Board Member, Cavic, Milena, Editorial Board Member, Chakrabarti, Sakti, Editorial Board Member, Cho, William C. S., Editorial Board Member, Czarnecka, Anna M., Editorial Board Member, Domingues, Cátia, Editorial Board Member, Eşkazan, A. Emre, Editorial Board Member, Fares, Jawad, Editorial Board Member, Fonseca Alves, Carlos E., Editorial Board Member, Fru, Pascaline, Editorial Board Member, Da Gama Duarte, Jessica, Editorial Board Member, García, Mónica C., Editorial Board Member, Gener, Melissa A.H., Editorial Board Member, Estrada Guadarrama, José Antonio, Editorial Board Member, Hargadon, Kristian M., Editorial Board Member, Holvoet, Paul, Editorial Board Member, Jurisic, Vladimir, Editorial Board Member, Kabir, Yearul, Editorial Board Member, Katsila, Theodora, Editorial Board Member, Kleeff, Jorg, Editorial Board Member, Liang, Chao, Editorial Board Member, Tan, Mei Lan, Editorial Board Member, Li, Weijie, Editorial Board Member, Prado López, Sonia, Editorial Board Member, Macha, Muzafar A., Editorial Board Member, Malara, Natalia, Editorial Board Member, Orhan, Adile, Editorial Board Member, Prado-Garcia, Heriberto, Editorial Board Member, Pérez-Velázquez, Judith, Editorial Board Member, Rashed, Wafaa M., Editorial Board Member, Sanguedolce, Francesca, Editorial Board Member, Sorrentino, Rosalinda, Editorial Board Member, Shubina, Irina Zh., Editorial Board Member, de Araujo, Heloisa Sobreiro Selistre, Editorial Board Member, Torres-Suárez, Ana Isabel, Editorial Board Member, Włodarczyk, Jakub, Editorial Board Member, Yeong, Joe Poh Sheng, Editorial Board Member, Toscano, Marta A., Editorial Board Member, Wong, Tak-Wah, Editorial Board Member, Yin, Jun, Editorial Board Member, and Yu, Bin, Editorial Board Member
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- 2023
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38. Human STAT3 variants underlie autosomal dominant hyper-IgE syndrome by negative dominance
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Asano, Takaki, Khourieh, Joëlle, Zhang, Peng, Rapaport, Franck, Spaan, András N, Li, Juan, Lei, Wei-Te, Pelham, Simon J, Hum, David, Chrabieh, Maya, Han, Ji Eun, Guérin, Antoine, Mackie, Joseph, Gupta, Sudhir, Saikia, Biman, Baghdadi, Jamila EI, Fadil, Ilham, Bousfiha, Aziz, Habib, Tanwir, Marr, Nico, Ganeshanandan, Luckshman, Peake, Jane, Droney, Luke, Williams, Andrew, Celmeli, Fatih, Hatipoglu, Nevin, Ozcelik, Tayfun, Picard, Capucine, Abel, Laurent, Tangye, Stuart G, Boisson-Dupuis, Stéphanie, Zhang, Qian, Puel, Anne, Béziat, Vivien, Casanova, Jean-Laurent, and Boisson, Bertrand
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Rare Diseases ,Genetics ,Biotechnology ,Aetiology ,2.1 Biological and endogenous factors ,Adolescent ,Adult ,Alleles ,Alternative Splicing ,Child ,Child ,Preschool ,Codon ,Nonsense ,Evolution ,Molecular ,Family ,Female ,Frameshift Mutation ,Genes ,Dominant ,Genetics ,Population ,HEK293 Cells ,Humans ,Infant ,Infant ,Newborn ,Job Syndrome ,Male ,Middle Aged ,Mutation ,Pedigree ,Protein Biosynthesis ,RNA ,Messenger ,STAT3 Transcription Factor ,Medical and Health Sciences ,Immunology - Abstract
Most patients with autosomal dominant hyper-IgE syndrome (AD-HIES) carry rare heterozygous STAT3 variants. Only six of the 135 in-frame variants reported have been experimentally shown to be dominant negative (DN), and it has been recently suggested that eight out-of-frame variants operate by haploinsufficiency. We experimentally tested these 143 variants, 7 novel out-of-frame variants found in HIES patients, and other STAT3 variants from the general population. Strikingly, all 15 out-of-frame variants were DN via their encoded (1) truncated proteins, (2) neoproteins generated from a translation reinitiation codon, and (3) isoforms from alternative transcripts or a combination thereof. Moreover, 128 of the 135 in-frame variants (95%) were also DN. The patients carrying the seven non-DN STAT3 in-frame variants have not been studied for other genetic etiologies. Finally, none of the variants from the general population tested, including an out-of-frame variant, were DN. Overall, our findings show that heterozygous STAT3 variants, whether in or out of frame, underlie AD-HIES through negative dominance rather than haploinsufficiency.
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- 2021
39. An Information Theoretic Exploration of Constrained MSSM
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Gupta, Surabhi and Gupta, Sudhir Kumar
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High Energy Physics - Phenomenology - Abstract
We discuss information theory as a tool to investigate constrained minimal supersymmetric Standard Model (CMSSM) in the light of observation of Higgs boson at the Large Hadron Collider. The entropy of the Higgs boson using its various detection modes has been constructed as a measure of the information and has been utilized to explore a wide range of CMSSM parameter space after including various experimental constraints from the LEP data, B-physics, electroweak precision observables and relic density of dark matter. According to our study while the lightest neutralino is preferred to have a mass around 1.92 TeV, the gluino mass is estimated to be around 7.44 TeV. The values of CMSSM parameters $m_0$, $m_{1/2}$, $A_0$ and $\tan\beta$ correspond to the most preferred scenario are found to be about 6 TeV, 3.6 TeV, $-$6.9 TeV and 36.8 respectively., Comment: 16 pages, 7 figures, 3 tables
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- 2020
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40. Collagenous Gastritis in Primary Selective IgM Deficiency: Transition to EBV+ Gastric Adenocarcinoma
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Narsai, Tejal, Su, Houfen, Braxton, David, and Gupta, Sudhir
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Biomedical and Clinical Sciences ,Immunology ,Digestive Diseases ,Rare Diseases ,Cancer - Abstract
Selective IgM deficiency (SIgMD) and isolated collagenous gastritis are two independent rare disorders. Our purpose is to report the 1st case of SIgMD and isolated collagenous gastritis and collagenous gastritis that has transitioned to EBV + gastric adenocarcinoma. Gastric biopsy tissue was analyzed by EBV-related encoded RNA in situ hybridization assay. Subsets of CD4, CD8, T follicular helper cells (TFH), and members of the "regulatory lymphocytes club" were measured with multiple panels of monoclonal antibodies and isotype controls by multicolor flow cytometry. The patient was diagnosed with SIgMD (extremely low serum IgM 9 mg/dl and normal IgG and IgA and exclusion of secondary causes of low IgM). Soon after SIgMD diagnosis, the patient developed collagenous gastritis and, 8 years later, developed gastric adenocarcinoma that was positive for EBV. An extensive immunological analysis revealed reduced naïve CD4 and CD8 effector memory T cells and increased naïve and central memory CD8 T cells. Among the circulating follicular helper T cells (cTFH), TFH1 and TFH2 were increased whereas TFH17 was decreased. CD4 Treg cells and TFR cells were increased, whereas Breg and CD8 Treg were comparable to control. In conclusion, SIgMD may be associated with isolated collagenous gastritis, and collagenous gastritis may transition to EBV + gastric adenocarcinoma. A role of regulatory lymphocytes in gastric cancer is discussed.
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- 2021
41. Coronavirus disease 2019 in patients with inborn errors of immunity: An international study
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Meyts, Isabelle, Bucciol, Giorgia, Quinti, Isabella, Neven, Bénédicte, Fischer, Alain, Seoane, Elena, Lopez-Granados, Eduardo, Gianelli, Carla, Robles-Marhuenda, Angel, Jeandel, Pierre-Yves, Paillard, Catherine, Sankaran, Vijay G, Demirdag, Yesim Yilmaz, Lougaris, Vassilios, Aiuti, Alessandro, Plebani, Alessandro, Milito, Cinzia, Dalm, Virgil ASH, Guevara-Hoyer, Kissy, Sánchez-Ramón, Silvia, Bezrodnik, Liliana, Barzaghi, Federica, Gonzalez-Granado, Luis Ignacio, Hayman, Grant R, Uzel, Gulbu, Mendonça, Leonardo Oliveira, Agostini, Carlo, Spadaro, Giuseppe, Badolato, Raffaele, Soresina, Annarosa, Vermeulen, François, Bosteels, Cedric, Lambrecht, Bart N, Keller, Michael, Mustillo, Peter J, Abraham, Roshini S, Gupta, Sudhir, Ozen, Ahmet, Karakoc-Aydiner, Elif, Baris, Safa, Freeman, Alexandra F, Yamazaki-Nakashimada, Marco, Scheffler-Mendoza, Selma, Espinosa-Padilla, Sara, Gennery, Andrew R, Jolles, Stephen, Espinosa, Yazmin, Poli, M Cecilia, Fieschi, Claire, Hauck, Fabian, Cunningham-Rundles, Charlotte, Mahlaoui, Nizar, Errors of Immunity, IUIS Committee of Inborn, Warnatz, Klaus, Sullivan, Kathleen E, and Tangye, Stuart G
- Subjects
Vaccine Related ,Clinical Research ,Pneumonia & Influenza ,Prevention ,Pediatric ,Emerging Infectious Diseases ,Pneumonia ,Infectious Diseases ,Lung ,Biodefense ,Rare Diseases ,Good Health and Well Being ,Adolescent ,Adult ,Aged ,COVID-19 ,Child ,Child ,Preschool ,Female ,Genetic Diseases ,Inborn ,Humans ,Immunologic Deficiency Syndromes ,Infant ,Infant ,Newborn ,Male ,Middle Aged ,Retrospective Studies ,Risk Factors ,SARS-CoV-2 ,Severity of Illness Index ,Young Adult ,primary immunodeficiencies ,inborn errors of immunity ,hypogammaglobulinemia ,immune dysregulation ,IUIS Committee of Inborn Errors of Immunity ,Immunology ,Allergy - Abstract
BackgroundThere is uncertainty about the impact of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection in individuals with rare inborn errors of immunity (IEI), a population at risk of developing severe coronavirus disease 2019. This is relevant not only for these patients but also for the general population, because studies of IEIs can unveil key requirements for host defense.ObjectiveWe sought to describe the presentation, manifestations, and outcome of SARS-CoV-2 infection in IEI to inform physicians and enhance understanding of host defense against SARS-CoV-2.MethodsAn invitation to participate in a retrospective study was distributed globally to scientific, medical, and patient societies involved in the care and advocacy for patients with IEI.ResultsWe gathered information on 94 patients with IEI with SARS-CoV-2 infection. Their median age was 25 to 34 years. Fifty-three patients (56%) suffered from primary antibody deficiency, 9 (9.6%) had immune dysregulation syndrome, 6 (6.4%) a phagocyte defect, 7 (7.4%) an autoinflammatory disorder, 14 (15%) a combined immunodeficiency, 3 (3%) an innate immune defect, and 2 (2%) bone marrow failure. Ten were asymptomatic, 25 were treated as outpatients, 28 required admission without intensive care or ventilation, 13 required noninvasive ventilation or oxygen administration, 18 were admitted to intensive care units, 12 required invasive ventilation, and 3 required extracorporeal membrane oxygenation. Nine patients (7 adults and 2 children) died.ConclusionsThis study demonstrates that (1) more than 30% of patients with IEI had mild coronavirus disease 2019 (COVID-19) and (2) risk factors predisposing to severe disease/mortality in the general population also seemed to affect patients with IEI, including more younger patients. Further studies will identify pathways that are associated with increased risk of severe disease and are nonredundant or redundant for protection against SARS-CoV-2.
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- 2021
42. SARS-CoV-2-Associated T-Cell Responses in the Presence of Humoral Immunodeficiency.
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Gupta, Sudhir, Su, Houfen, Narsai, Tejal, and Agrawal, Sudhanshu
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B-Lymphocyte Subsets ,T-Lymphocyte Subsets ,Humans ,Common Variable Immunodeficiency ,Immunocompetence ,Adult ,Middle Aged ,Female ,Male ,COVID-19 ,SARS-CoV-2 ,Follicular helper T cells ,Germinal center B cells ,Memory T and B cells ,Plasmablasts ,Regulatory lymphocytes ,Immunology ,Allergy - Abstract
We report perhaps the most comprehensive study of subsets of CD4+ and CD8+ and subsets of B cells in a mild symptomatic SARS-CoV-2+ immunocompetent patient and a common variable immunodeficiency disease (CVID) patient who had normal absolute lymphocyte counts and remained negative for SARS-CoV-2 IgG antibodies. Naïve (TN), central memory (TCM), effector memory (TEM), and terminally differentiated effector memory (TEMRA) subsets of CD4+ and CD8+ T cells, subsets of T follicular helper cells (cTFH, TFH1, TFH2, TFH17, TFH1/TFH17, and TFR), CD4 Treg, CD8 Treg, mature B cells, transitional B cells, marginal zone B cells, germinal center (GC) B cells, CD21low B cells, antibody-secreting cells (plasmablasts), and Breg cells were examined in patients and age-matched controls with appropriate monoclonal antibodies and isotype controls using multicolor flow cytometry. Different patterns of abnormalities (often contrasting) were observed in the subsets of CD4+ T, CD8+ T, B-cell subsets, and regulatory lymphocytes among the immunocompetent patient and CVID patient as compared to corresponding healthy controls. Furthermore, when data were analyzed between the 2 patients, the immunocompetent patient demonstrated greater changes in various subsets as compared to the CVID patient. These data demonstrate different immunological responses to SARS-CoV-2 infection in an immunocompetent patient and the CVID patient. A marked decrease in GC B cells and plasmablasts may be responsible for failure to make SARS-CoV-2 antibodies. The lack of SARS-CoV-2 antibodies with mild clinical disease suggests an important role of T-cell response in defense against SARS-CoV-2 infection.
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- 2021
43. Human pregnancy levels of estrogen and progesterone contribute to humoral immunity by activating TFH/B cell axis
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Monteiro, Clarice, Kasahara, Taissa, Sacramento, Priscila M, Dias, Aleida, Leite, Simone, Silva, Vander G, Gupta, Sudhir, Agrawal, Anshu, and Bento, Cleonice AM
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Biomedical and Clinical Sciences ,Immunology ,Infectious Diseases ,Estrogen ,Aetiology ,2.1 Biological and endogenous factors ,Good Health and Well Being ,Adult ,B-Lymphocyte Subsets ,B-Lymphocytes ,Cell Differentiation ,Cytokines ,Estradiol ,Female ,Hepatitis B Vaccines ,Humans ,Immunity ,Humoral ,Immunoglobulin G ,In Vitro Techniques ,Interleukins ,Pregnancy ,Progesterone ,T Follicular Helper Cells ,Young Adult ,anti-HBsAg IgG ,B cells ,estrogen ,progesterone ,T(FH)cells ,TFH cells - Abstract
Circulating TFH (cTFH ) cells express CXCR5, PD-1, and, when activated, ICOS, and release IL-21. According to the production of IFN-γ, IL-4, and IL-17 and expression of FoxP3, these cells are also classified as cTFH 1, cTFH 2, cTFH 17, and cTFR cells, respectively. This CD4+ T-cell subset is pivotal to efficient humoral immunity, and pregnancy appears to favor IgG production. Here, not only pregnancy amplified the in vivo production of anti-HBsAg IgG in HBV immunized women, but the frequency of cTFH cells was directly correlated with estradiol levels. In vitro, pregnancy-related dose of 17-β-estradiol (E2) directly increased the percentage of different cTFH subsets. While E2 and progesterone (P4) increased the proportion of differentiated TFH cells derived from naïve CD4+ T-cells, only E2 amplified the release of IL-21 in those cell cultures. In addition, E2 and P4 increased the proportion of memory B cells and plasma cells, respectively. In SEB-activated B/TFH cell co-cultures, E2, in the presence of P4, increased the production of total IgG. Finally, among the hormones, P4 was stronger in upregulating the percentage of IL-10+ TFR cells. Collectively, our findings suggested that E2 and P4 cooperate in the humoral immune response by favoring the expansion of different cTFH and B cell subsets.
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- 2021
44. Update on Infections in Primary Antibody Deficiencies
- Author
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Demirdag, Yesim Yilmaz and Gupta, Sudhir
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Biomedical and Clinical Sciences ,Clinical Sciences ,Infectious Diseases ,Prevention ,Infection ,Agammaglobulinemia ,Animals ,Bacterial Infections ,Class I Phosphatidylinositol 3-Kinases ,Common Variable Immunodeficiency ,Humans ,Immunocompromised Host ,Immunoglobulins ,Primary Immunodeficiency Diseases ,Prognosis ,Respiratory Tract Infections ,Risk Assessment ,Risk Factors ,primary immunodeficiencies ,infections ,common variable immunodeficiency ,immunoglobulin therapy ,selective IgA deficiency ,specific antibody deficiency ,IgG subclass deficiency ,selective IgM deficiency ,Immunology ,Medical Microbiology ,Biochemistry and cell biology ,Genetics - Abstract
Bacterial respiratory tract infections are the hallmark of primary antibody deficiencies (PADs). Because they are also among the most common infections in healthy individuals, PADs are usually overlooked in these patients. Careful evaluation of the history, including frequency, chronicity, and presence of other infections, would help suspect PADs. This review will focus on infections in relatively common PADs, discussing diagnostic challenges, and some management strategies to prevent infections.
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- 2021
45. Editorial: Advances in Primary Immunodeficiencies in India
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Gupta, Sudhir and Sehgal, Shobha
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Humans ,India ,Primary Immunodeficiency Diseases ,prenatal diagnosis ,SCID ,XLA ,WAS ,LAD ,MSMD ,HSCT ,FLH ,Immunology ,Medical Microbiology - Published
- 2021
46. Human pregnancy levels of estrogen and progesterone contribute to humoral immunity by activating TFH /B cell axis.
- Author
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Monteiro, Clarice, Kasahara, Taissa, Sacramento, Priscila M, Dias, Aleida, Leite, Simone, Silva, Vander G, Gupta, Sudhir, Agrawal, Anshu, and Bento, Cleonice AM
- Subjects
B cells ,TFH cells ,anti-HBsAg IgG ,estrogen ,progesterone ,T(FH)cells ,Immunology - Abstract
Circulating TFH (cTFH ) cells express CXCR5, PD-1, and, when activated, ICOS, and release IL-21. According to the production of IFN-γ, IL-4, and IL-17 and expression of FoxP3, these cells are also classified as cTFH 1, cTFH 2, cTFH 17, and cTFR cells, respectively. This CD4+ T-cell subset is pivotal to efficient humoral immunity, and pregnancy appears to favor IgG production. Here, not only pregnancy amplified the in vivo production of anti-HBsAg IgG in HBV immunized women, but the frequency of cTFH cells was directly correlated with estradiol levels. In vitro, pregnancy-related dose of 17-β-estradiol (E2) directly increased the percentage of different cTFH subsets. While E2 and progesterone (P4) increased the proportion of differentiated TFH cells derived from naïve CD4+ T-cells, only E2 amplified the release of IL-21 in those cell cultures. In addition, E2 and P4 increased the proportion of memory B cells and plasma cells, respectively. In SEB-activated B/TFH cell co-cultures, E2, in the presence of P4, increased the production of total IgG. Finally, among the hormones, P4 was stronger in upregulating the percentage of IL-10+ TFR cells. Collectively, our findings suggested that E2 and P4 cooperate in the humoral immune response by favoring the expansion of different cTFH and B cell subsets.
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- 2021
47. The urgent need for integrated science to fight COVID-19 pandemic and beyond
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Moradian, Negar, Ochs, Hans D, Sedikies, Constantine, Hamblin, Michael R, Camargo, Carlos A, Martinez, J Alfredo, Biamonte, Jacob D, Abdollahi, Mohammad, Torres, Pedro J, Nieto, Juan J, Ogino, Shuji, Seymour, John F, Abraham, Ajith, Cauda, Valentina, Gupta, Sudhir, Ramakrishna, Seeram, Sellke, Frank W, Sorooshian, Armin, Wallace Hayes, A, Martinez-Urbistondo, Maria, Gupta, Manoj, Azadbakht, Leila, Esmaillzadeh, Ahmad, Kelishadi, Roya, Esteghamati, Alireza, Emam-Djomeh, Zahra, Majdzadeh, Reza, Palit, Partha, Badali, Hamid, Rao, Idupulapati, Saboury, Ali Akbar, Jagan Mohan Rao, L, Ahmadieh, Hamid, Montazeri, Ali, Fadini, Gian Paolo, Pauly, Daniel, Thomas, Sabu, Moosavi-Movahed, Ali A, Aghamohammadi, Asghar, Behmanesh, Mehrdad, Rahimi-Movaghar, Vafa, Ghavami, Saeid, Mehran, Roxana, Uddin, Lucina Q, Von Herrath, Matthias, Mobasher, Bahram, and Rezaei, Nima
- Subjects
Biomedical and Clinical Sciences ,Health Sciences ,Infection ,Good Health and Well Being ,Betacoronavirus ,Biomedical Research ,COVID-19 ,Coronavirus Infections ,Delivery of Health Care ,Integrated ,Emergencies ,Health Services Needs and Demand ,History ,21st Century ,Humans ,Interdisciplinary Communication ,Interdisciplinary Studies ,Pandemics ,Pneumonia ,Viral ,Public Health ,SARS-CoV-2 ,Coronavirus ,Complex problems ,Collaboration ,Interdisciplinarity ,Public health ,Medical and Health Sciences ,Immunology ,Biomedical and clinical sciences ,Health sciences - Abstract
The COVID-19 pandemic has become the leading societal concern. The pandemic has shown that the public health concern is not only a medical problem, but also affects society as a whole; so, it has also become the leading scientific concern. We discuss in this treatise the importance of bringing the world's scientists together to find effective solutions for controlling the pandemic. By applying novel research frameworks, interdisciplinary collaboration promises to manage the pandemic's consequences and prevent recurrences of similar pandemics.
- Published
- 2020
48. T-odd anomalous interactions of the top-quark at the Large Hadron Collider
- Author
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Tiwari, Apurba and Gupta, Sudhir Kumar
- Subjects
High Energy Physics - Phenomenology ,High Energy Physics - Experiment - Abstract
We study the effects of T-odd interactions of top-quark via the pair production of top-quark in the semileptonic detection modes at the Large Hadron Collider by means of the T-odd observables constructed through the momenta of the observed decay products of the top (and anti-top)-quark for a wide range of CP-violating scale $\Lambda$. Estimates on the sensitivities of the coupling strength of such interactions for 13 TeV LHC energy with $\int \mathcal L dt$ = 36.1 fb$^{-1}$, 140 fb$^{-1}$ and for HL-LHC with 14 TeV energy with integrated luminosities of 0.3 ab$^{-1}$, 1 ab$^{-1}$, 2 ab$^{-1}$ and 3 ab$^{-1}$ are also presented for $\Lambda$ ranging between $M_W$ and 2 TeV., Comment: 13 Pages and 10 figures
- Published
- 2019
- Full Text
- View/download PDF
49. Chemically modified jackfruit leaves as a low-cost agro-waste adsorbent for Pb(II) removal from synthetic wastewater
- Author
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Gupta, Ritu, Gupta, Sudhir Kumar, Gehlot, Chhagan Lal, and Bahadur, Indra
- Published
- 2023
- Full Text
- View/download PDF
50. Phenotypically defined subpopulations of circulating follicular helper T cells in common variable immunodeficiency
- Author
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Yesillik, Sait and Gupta, Sudhir
- Subjects
Biomedical and Clinical Sciences ,Immunology ,Vaccine Related ,Aetiology ,2.1 Biological and endogenous factors ,Good Health and Well Being ,Adolescent ,Adult ,Aged ,Aged ,80 and over ,Common Variable Immunodeficiency ,Female ,Germinal Center ,Humans ,Male ,Middle Aged ,Receptors ,CXCR5 ,T Follicular Helper Cells ,T-Lymphocytes ,Helper-Inducer ,Young Adult ,autoimmunity ,CVID ,follicular helper T cells - Abstract
BackgroundCommon variable immunodeficiency (CVID) is characterized by low immunoglobulin G and IgA/IgM, decreased switched memory B cells, impaired response to vaccine, and an increased susceptibility to infections and autoimmunity. TFH cells play an important role in germinal center reaction where it supports isotype switching, somatic hypermutation, generation of memory B cells, and differentiation of B cells to plasma cells. The objective was to study the distribution of three subsets of TFH cells and their relationship with autoimmune diseases associated with CVID.MethodsTFH cells have been divided into TFH 1 (interleukin 21 [IL-21] and interferon γ), TFH 2 (IL-21 and IL-4), and TFH 17 (IL-21 and IL-17) cells. Mononuclear cells from 25 patients with CVID and age and gender-matched controls were stained with various monoclonal antibodies (anti-CD4 APC, anti-CXCR5 FITC, anti-CCR6 PerCP, and anti-CXCR3 PE) and isotype controls and analyzed for TFH 1 (CD4+ CXCR5+ CXCR3+ CCR6- ), TFH 2 (CD4+ CXCR5+ CXCR3- CCR6- ), and TFH 17 (CD4+ CXCR5+ CXCR3- CCR6+ ) cells by multicolor flow cytometry. Twenty thousand cells were acquired and analyzed by FlowJo software. Statistical analysis of comparison of patients and healthy controls was performed by paired t test using PRISM 7 software.ResultsTFH 2 and TFH 17 cells subpopulations of TFH cells were significantly decreased (P
- Published
- 2020
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