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2. UVR and Vitamin D Synthesis

Author

Chopra, Deepti, Kumar, Dhanananajay, Dubey, Divya, Singh, Jyoti, Srivastav, Ajeet Kumar, Gupta, Kailash Chand, Dwivedi, Ashish, editor, Agarwal, Neeraj, editor, Ray, Lipika, editor, and Tripathi, Amit Kumar, editor

Published
2019
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4. Protective Role of Phytochemicals Against UVR

Author

Chopra, Deepti, Singh, Jyoti, Srivastav, Ajeet Kumar, Dubey, Divya, Ray, Ratan Singh, Gupta, Kailash Chand, Ray, Ratan Singh, editor, Haldar, Chandana, editor, Dwivedi, Ashish, editor, Agarwal, Neeraj, editor, and Singh, Jyoti, editor

Published
2018
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7. Conventional and molecular breeding for disease resistance in chickpea: status and strategies.

Author

Choudhary, Arbind K., Jain, Shailesh Kumar, Dubey, Abhishek Kumar, Kumar, Jitendra, Sharma, Mamta, Gupta, Kailash Chand, Sharma, Leela Dhar, Prakash, Ved, and Kumar, Saurabh

Abstract

Chickpea (Cicer arietinum L.) is an important grain legume at the global level. Among different biotic stresses, diseases are the most important factor limiting its production, causing yield losses up to 100% in severe condition. The major diseases that adversely affect yield of chickpea include Fusarium wilt, Ascochyta blight and Botrytis gray mold. However, dry root rot, collar rot, Sclerotinia stem rot, rust, stunt disease and phyllody have been noted as emerging biotic threats to chickpea production in many production regions. Identification and incorporation of different morphological and biochemical traits are required through breeding to enhance genetic gain for disease resistance. In recent years, remarkable progress has been made in the development of trait-specific breeding lines, genetic and genomic resources in chickpea. Advances in genomics technologies have opened up new avenues to introgress genes from secondary and tertiary gene pools for improving disease resistance in chickpea. In this review, we have discussed important diseases, constraints and improvement strategies for enhancing disease resistance in chickpea. [ABSTRACT FROM AUTHOR]

Published
2023
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15. Synthesis of PLGA nanoparticles of tea polyphenols and their strong in vivo protective effect against chemically induced DNA damage [Erratum]

Author

Srivastava,Amit Kumar, Bhatnagar,Priyanka, Singh,Madhulika, Mishra,Sanjay, Kumar,Pradeep, Shukla,Yogeshwer, Gupta,Kailash Chand, Srivastava,Amit Kumar, Bhatnagar,Priyanka, Singh,Madhulika, Mishra,Sanjay, Kumar,Pradeep, Shukla,Yogeshwer, and Gupta,Kailash Chand

Abstract

Srivastava AK, Bhatnagar P, Singh M, et al. Int J Nanomedicine. 2013;8:1451–1462.Upon reviewing the article, the authors noticed the PCR gel images were difficult to view in Figures 6 and 7 (pages 1458 and 1459). The authors confirm that the higher resolution figures provided do not impact the findings of the study. Figures 6 and 7 should be presented as follows:Read the original article

Published
2019

16. PLGA-Encapsulated Tea Polyphenols Enhance The Chemotherapeutic Efficacy Of Cisplatin Against Human Cancer Cells And Mice Bearing Ehrlich Ascites Carcinoma [Retraction]

Author

Singh,Madhulika, Bhatnagar,Priyanka, Mishra,Sanjay, Kumar,Pradeep, Shukla,Yogeshwer, Gupta,Kailash Chand, Singh,Madhulika, Bhatnagar,Priyanka, Mishra,Sanjay, Kumar,Pradeep, Shukla,Yogeshwer, and Gupta,Kailash Chand

Abstract

Singh M, Bhatnagar P, Mishra S, Kumar P, Shukla Y, Gupta KC. PLGA-encapsulated tea polyphenols enhance the chemotherapeutic efficacy of cisplatin against human cancer cells and mice bearing Ehrlich ascites carcinoma. International Journal of Nanomedicine. 2015;10:6789–6809.The Editor-in-Chief and Publisher of International Journal of Nanomedicine wish to retract the published article.Concerns were raised about the possibility of image manipulation and duplication in the published article. The alleged image manipulation relates to the Western blot bands presented in:● Figure 6A p-NF-κB and p-IκB-α● Figure 6A, Figure S3A and S3C β-actin bands● Figure S3C Bax and Bcl-2It was also found that images in Figure 4 (TF-NP + CDDP; EGCG + CDDP and EGCG-NP + CDDP) were duplicated from images presented in Figure 5 of the article: Singh M, Bhui K, Singh R, Shukla Y. Tea polyphenols enhance cisplatin chemosensitivity in cervical cancer cells via induction of apoptosis. Life Sciences. 2013;93:7–16. doi: 10.1016/j.lfs.2013.02.001.The authors were contacted but were unable to provide a satisfactory explanation for the alleged image duplication and could not supply satisfactory revisions for the article. The Director of CSIR-Indian Institute of Toxicology Research (CSIR-IITR) was contacted and was very helpful throughout our investigation. The Editor-in-Chief determined the findings and conclusions presented in the study were no longer upported.This retraction relates to this paper

Published
2019

19. Correction to Trans-Blood Brain Barrier Delivery of Dopamine-Loaded Nanoparticles Reverses Functional Deficits in Parkinsonian Rats

Author

Pahuja, Richa, primary, Seth, Kavita, additional, Shukla, Anshi, additional, Shukla, Rajendra Kumar, additional, Bhatnagar, Priyanka, additional, Chauhan, Lalit Kumar Singh, additional, Saxena, Prem Narain, additional, Arun, Jharna, additional, Chaudhari, Bhushan Pradosh, additional, Patel, Devendra Kumar, additional, Singh, Sheelendra Pratap, additional, Shukla, Rakesh, additional, Khanna, Vinay Kumar, additional, Kumar, Pradeep, additional, Chaturvedi, Rajnish Kumar, additional, and Gupta, Kailash Chand, additional

Published
2019
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20. Correction to Curcumin-Loaded Nanoparticles Potently Induce Adult Neurogenesis and Reverse Cognitive Deficits in Alzheimer’s Disease Model via Canonical Wnt/β-Catenin Pathway

Author

Tiwari, Shashi Kant, primary, Agarwal, Swati, additional, Seth, Brashket, additional, Yadav, Anuradha, additional, Nair, Saumya, additional, Bhatnagar, Priyanka, additional, Karmakar, Madhumita, additional, Kumari, Manisha, additional, Chauhan, Lalit Kumar Singh, additional, Patel, Devendra Kumar, additional, Srivastava, Vikas, additional, Singh, Dhirendra, additional, Gupta, Shailendra Kumar, additional, Tripathi, Anurag, additional, Chaturvedi, Rajnish Kumar, additional, and Gupta, Kailash Chand, additional

Published
2019
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21. ERRATUM Mechanism of Nanotization-Mediated Improvement in the Efficacy of Caffeine Against 1-Methyl-4-Phenyl-1,2,3,6-Tetrahydropyridine-Induced Parkinsonism (Journal of Biomedical Nanotechnology, Vol. 11(12), pp. 2211–2222 (2015))

Author

Singhal, Naveen Kumar, primary, Agarwal, Swati, additional, Bhatnagar, Priyanka, additional, Tiwari, Manindra Nath, additional, Tiwari, Shashi Kant, additional, Srivastava, Garima, additional, Kumar, Pradeep, additional, Seth, Brashket, additional, Patel, Devendra Kumar, additional, Chaturvedi, Rajnish Kumar, additional, Singh, Mahendra Pratap, additional, and Gupta, Kailash Chand, additional

Published
2019
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26. PLGA-encapsulated tea polyphenols enhance the chemotherapeutic efficacy of cisplatin against human cancer cells and mice bearing Ehrlich ascites carcinoma

Author

Singh,Madhulika, Bhatnagar,Priyanka, Mishra,Sanjay, Kumar,Pradeep, Shukla,Yogeshwer, Gupta,Kailash Chand, Singh,Madhulika, Bhatnagar,Priyanka, Mishra,Sanjay, Kumar,Pradeep, Shukla,Yogeshwer, and Gupta,Kailash Chand

Abstract

This paper has been retracted Madhulika Singh,1 Priyanka Bhatnagar,2 Sanjay Mishra,1 Pradeep Kumar,2 Yogeshwer Shukla,1 Kailash Chand Gupta1,2 1CSIR-Indian Institute of Toxicology Research, Lucknow, 2CSIR-Institute of Genomics and Integrative Biology, Delhi University Campus, Delhi, India Abstract: The clinical success of the applicability of tea polyphenols awaits efficient systemic delivery and bioavailability. Herein, following the concept of nanochemoprevention, which uses nanotechnology for enhancing the efficacy of chemotherapeutic drugs, we employed tea polyphenols, namely theaflavin (TF) and epigallocatechin-3-gallate (EGCG) encapsulated in a biodegradable nanoparticulate formulation based on poly(lactide-co-glycolide) (PLGA) with approximately 26% and 18% encapsulation efficiency, respectively. It was observed that TF/EGCG encapsulated PLGA nanoparticles (NPs) offered an up to ~7-fold dose advantage when compared with bulk TF/EGCG in terms of exerting its antiproliferative effects and also enhanced the anticancer potential of cisplatin (CDDP) in A549 (lung carcinoma), HeLa (cervical carcinoma), and THP-1 (acute monocytic leukemia) cells. Cell cycle analysis revealed that TF/EGCG-NPs were more efficient than bulk TF/EGCG in sensitizing A549 cells to CDDP-induced apoptosis, with a dose advantage of up to 20-fold. Further, TF/EGCG-NPs, alone or in combination with CDDP, were more effective in inhibiting NF-κB activation and in suppressing the expression of cyclin D1, matrix metalloproteinase-9, and vascular endothelial growth factor, involved in cell proliferation, metastasis, and angiogenesis, respectively. EGCG and TF-NPs were also found to be more effective than bulk TF/EGCG in inducing the cleavage of caspase-3 and caspase-9 and Bax/Bcl2 ratio in favor of apoptosis. Further, in vivo evaluation of these NPs in combination with CDDP showed an increase in life span (P<0.05) in mice bearing Ehrlich’s ascites carcino

Published
2015

27. Mechanism of Nanotization-Mediated Improvement in the Efficacy of Caffeine Against 1-Methyl-4-Phenyl-1,2,3,6-Tetrahydropyridine-Induced Parkinsonism

Author

Singhal, Naveen Kumar, primary, Agarwal, Swati, additional, Bhatnagar, Priyanka, additional, Tiwari, Manindra Nath, additional, Tiwari, Shashi Kant, additional, Srivastava, Garima, additional, Kumar, Pradeep, additional, Seth, Brashket, additional, Patel, Devendra Kumar, additional, Chaturvedi, Rajnish Kumar, additional, Singh, Mahendra Pratap, additional, and Gupta, Kailash Chand, additional

Published
2015
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29. Trans-Blood Brain Barrier Delivery of Dopamine-Loaded Nanoparticles Reverses Functional Deficits in Parkinsonian Rats

Author

Pahuja, Richa, primary, Seth, Kavita, additional, Shukla, Anshi, additional, Shukla, Rajendra Kumar, additional, Bhatnagar, Priyanka, additional, Chauhan, Lalit Kumar Singh, additional, Saxena, Prem Narain, additional, Arun, Jharna, additional, Chaudhari, Bhushan Pradosh, additional, Patel, Devendra Kumar, additional, Singh, Sheelendra Pratap, additional, Shukla, Rakesh, additional, Khanna, Vinay Kumar, additional, Kumar, Pradeep, additional, Chaturvedi, Rajnish Kumar, additional, and Gupta, Kailash Chand, additional

Published
2015
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35. Synthesis of PLGA nanoparticles of tea polyphenols and their strong in vivo protective effect against chemically induced DNA damage

Author

Srivastava,Amit Kumar, Bhatnagar,Priyanka, Singh,Madhulika, Mishra,Sanjay, Kumar,Pradeep, Shukla,Yogeshwer, Gupta,Kailash Chand, Srivastava,Amit Kumar, Bhatnagar,Priyanka, Singh,Madhulika, Mishra,Sanjay, Kumar,Pradeep, Shukla,Yogeshwer, and Gupta,Kailash Chand

Abstract

Amit Kumar Srivastava,1 Priyanka Bhatnagar,2 Madhulika Singh,1 Sanjay Mishra,1 Pradeep Kumar,2 Yogeshwer Shukla,1 Kailash Chand Gupta1,2 1Proteomics Laboratory, Indian Institute of Toxicology Research (CSIR), Lucknow, India; 2Nucleic Acid Research Laboratory, Institute of Genomics and Integrative Biology (CSIR), Delhi University Campus, India Abstract: In spite of proficient results of several phytochemicals in preclinical settings, the conversion rate from bench to bedside is not very encouraging. Many reasons are attributed to this limited success, including inefficient systemic delivery and bioavailability under in vivo conditions. To achieve improved efficacy, polyphenolic constituents of black (theaflavin [TF]) and green (epigallocatechin-3-gallate [EGCG]) tea in poly(lactide-co-glycolide) nanoparticles (PLGA-NPs) were entrapped with entrapment efficacy of ~18% and 26%, respectively. Further, their preventive potential against 7,12-dimethylbenzanthracene (DMBA)-induced DNA damage in mouse skin using DNA alkaline unwinding assay was evaluated. Pretreatment (topically) of mouse skin with either TF or EGCG (100 µg/mouse) doses exhibits protection of 45.34% and 28.32%, respectively, against DMBA-induced DNA damage. However, pretreatment with TF-loaded PLGA-NPs protects against DNA damage 64.41% by 1/20th dose of bulk, 71.79% by 1/10th dose of bulk, and 72.46% by 1/5th dose of bulk. Similarly, 51.28% (1/20th of bulk), 57.63% (1/10th of bulk), and 63.14% (1/5th of bulk) prevention was noted using EGCG-loaded PLGA-NP doses. These results showed that tea polyphenol-loaded PLGA-NPs have ~30-fold dose-advantage than bulk TF or EGCG doses. Additionally, TF- or EGCG-loaded PLGA-NPs showed significant potential for induction of DNA repair genes (XRCC1, XRCC3, and ERCC3) and suppression of DNA damage responsive genes (p53, p21, MDM2, GADD45α, and COX-2) as compared with respective bulk TF or EGCG doses. Taken together, TF- or EGCG-loaded PLGA-NPs showed a

Published
2013

36. Curcumin-Loaded Nanoparticles Potently Induce Adult Neurogenesis and Reverse Cognitive Deficits in Alzheimer’s Disease Model via Canonical Wnt/β-Catenin Pathway

Author

Tiwari, Shashi Kant, primary, Agarwal, Swati, additional, Seth, Brashket, additional, Yadav, Anuradha, additional, Nair, Saumya, additional, Bhatnagar, Priyanka, additional, Karmakar, Madhumita, additional, Kumari, Manisha, additional, Chauhan, Lalit Kumar Singh, additional, Patel, Devendra Kumar, additional, Srivastava, Vikas, additional, Singh, Dhirendra, additional, Gupta, Shailendra Kumar, additional, Tripathi, Anurag, additional, Chaturvedi, Rajnish Kumar, additional, and Gupta, Kailash Chand, additional

Published
2013
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45. Size dependent toxicity of zinc oxide nano-particles in soil nematode Caenorhabditis elegans.

Author

Khare, Priyanka, Sonane, Madhavi, Nagar, Yogendra, Moin, Nida, Ali, Shakir, Gupta, Kailash Chand, and Satish, Aruna

Subjects
ZINC oxide, NANOPARTICLES, APOPTOSIS inhibition, DRUG side effects, SOIL nematodes, CAENORHABDITIS elegans, HEALTH risk assessment
Abstract

Zinc oxide nano-particles (ZnO NPs), with their unique physico-chemical properties conferred by various size formulations, are extensively used in consumer products. The enormous usage coupled with their release to the environment demands risk assessment of ZnO NPs on health and the environment. Toxicity of ZnO NPs is well understood in comparison to the bulk ZnO. However, toxicity in relation to the NP size is poorly understood. In this context, we examined the adverse effects of different sizes (35 nm, 50 nm and 100 nm) of ZnO NPs in soil nematodeC. elegansalong with bulk ZnO and ZnCl2. Here, we show that growth, reproduction and behavior of worms were adversely affected by ZnO NPs in a size dependent manner. Further, exposure to ZnO NPs caused modulation of expression/function of genes associated with Insulin/IGF-like signaling pathway and/or stress response pathway in a size dependent manner in exposed worms. The expression of pro-apoptotic gene and suppression of anti-apoptotic genes, together with increased numbers of cell corpses in the germ line, indicated that apoptosis was also dependent on the size of the ZnO NP. Taken together, our study provides evidence that exposure to ZnO NPs disrupts various physiological processes and causes apoptosis in the germ-line even at very low concentration in a size dependent manner. Our finding suggests the inclusion of size as an additional measure for the cautious monitoring of ZnO NP disposal into the environment. [ABSTRACT FROM PUBLISHER]

Published
2015
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46. Choice of Polymer Matrix, Its Functionalization and Estimation of Functional Group Density for Preparation of Biochips.

Author

Walker, John M., Rampal, Jang B., Mahajan, Shweta, Vaijayanthi, Bhashyam, Rembhotkar, Gopal, Gupta, Kailash Chand, and Kumar, Pradeep

Abstract

Oligonucleotide microarray has become an important and powerful tool for various genomic analyses, where, unlike conventional methods, one can identify simultaneously a large number of targets in a given sample. Postsynthesis immobilization, the most widely used method, involves the noncovalent and covalent fixing of suitably modified oligonucleotides on the solid supports. Among the various functional groups aminoalkyl, hydroxyalkyl, mercaptoalkyl, aldehyde, epoxy, and carboxyl the most frequently used functional groups on the polymeric surfaces. Because glass and polypropylene, the most widely used materials, are nonporous in nature, the functional groups density on the surface remains very low. In order to know the exact concentration of a ligand to be immobilized, it is essential to estimate the accessible functional groups on these surfaces. For this purpose, sensitive methods are required to estimate exact density of available functional groups on the surfaces. Apart from physical methods, a number of sensitive chemical methods, by making use of high extinction coefficient of 4,4′-dimethoxytrityl cation (ε498 = 70,000 Lmol−11cm−1), have been reported to estimate accessible functional groups on the glass based polymer supports. In this chapter, use of these reagents for spectrophotometric determination of functional group density on glass microslides and polypropylene film has been discussed. [ABSTRACT FROM AUTHOR]

Published
2007
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47. Construction of Oligonucleotide Microarrays (Biochip) Using Heterobifunctional Reagents.

Author

Walker, John M., Rampal, Jang B., Choithani, Jyoti, Vaijayanthi, B., Kumar, Pradeep, and Gupta, Kailash Chand

Abstract

A number of hetero- and homobifunctional reagents have been reported to immobilize biomolecules on a variety of supports. However, efforts are on to search for a method, which is relatively simple, involving minimum of steps, cost effective, easy to reproduce, and that produces stable oligonucleotide arrays. Two new reagents, viz., [N-(2-trifluoroethanesulfonatoethyl)-N-(methyl)- triethoxysilylpropyl-3-amine], and [N-(3-trifluoroethanesulfonyloxypropyl)anthraquinone-2- carboxamide] have been designed considering the above points. These reagents contain different functional groups at their two ends. In [N-(2-trifluoroethanesulfonatoethyl)-N-(methyl)- triethoxysilylpropyl-3-amine], one end (triethoxysilyl) is capable of binding to the virgin glass surface and the other one consists of trifluoroethanesulfonate (tresyl) function specific toward aminoalkyl and mercaptoalkyl functionalities, which are easy to introduce at the 3′- or 5′-end of oligonucleotides. Likewise, in [N-(3-trifluoroethanesulfonyloxypropyl)anthraquinone-2- carboxamide], one end consists of photoactivatable moiety (anthraquinone) capable of reacting to a C-H containing surface and the tresyl function at the other end reacts specifically with aminoalkyl and mercaptoalkyl functionalities in modified oligonucleotides. These reagents have successfully been utilized to construct a number of oligonucleotide arrays and subsequently used for the detection of mismatches. [ABSTRACT FROM AUTHOR]

Published
2007
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49. Biocomposite Cryogels as Tissue-Engineered Biomaterials for Regeneration of Critical-Sized Cranial Bone Defects

Author

Mishra, Ruchi, Goel, Sudhir Kumar, Gupta, Kailash Chand, and Kumar, Ashok

Abstract

Analysis of the in vivoregeneration capability of any tissue-engineered biomaterial is necessary once it shows potential characteristics during in vitrostudies. Thus, we applied polyvinyl alcohol-tetraethylorthosilicate-alginate-calcium oxide (PTAC) biocomposite cryogel on critical-sized cranial bone defects in wistar rats for examining the comparative bone regeneration of cryogel-treated and nontreated defects over a period of 4 weeks. An in-depth analysis was performed from macroscopic level till the gene level. Bone regeneration in cryogel-treated defects was clearly evident from the results, whereas the nontreated group did not show any defect healing except at few peripheral areas. At the macroscopic level, micro-computed tomography analysis revealed new bone formation. This was further confirmed at the cellular level, wherein, new bone formation was demonstrated by hematoxylin and eosin staining. Osteoblastic differentiation was further validated by immunohistological staining of runt-related transcription factor-2 (Runx-2) protein and via calcium-phosphate crystal formation after 2 weeks through scanning electron microscopy and energy dispersive X-ray spectroscopy. Finally, at the gene level, real-time PCR analysis confirmed the mRNA expression of osteoblastic markers, that is, runx-2, collagen type I (Col I), alkaline phosphatase (ALP), and osteocalcin (OCN). Therefore, the results of in vivocranial defect model studies suggest that PTAC biocomposite cryogels can show suitable potential for human bone regeneration.

Published
2014
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50. Curcumin-Loaded Nanoparticles Potently Induce Adult Neurogenesis and Reverse Cognitive Deficits in Alzheimer’s Disease Model viaCanonical Wnt/β-Catenin Pathway

Author

Tiwari, Shashi Kant, Agarwal, Swati, Seth, Brashket, Yadav, Anuradha, Nair, Saumya, Bhatnagar, Priyanka, Karmakar, Madhumita, Kumari, Manisha, Chauhan, Lalit Kumar Singh, Patel, Devendra Kumar, Srivastava, Vikas, Singh, Dhirendra, Gupta, Shailendra Kumar, Tripathi, Anurag, Chaturvedi, Rajnish Kumar, and Gupta, Kailash Chand

Abstract

Neurogenesis, a process of generation of new neurons, is reported to be reduced in several neurodegenerative disorders including Alzheimer’s disease (AD). Induction of neurogenesis by targeting endogenous neural stem cells (NSC) could be a promising therapeutic approach to such diseases by influencing the brain self-regenerative capacity. Curcumin, a neuroprotective agent, has poor brain bioavailability. Herein, we report that curcumin-encapsulated PLGA nanoparticles (Cur-PLGA-NPs) potently induce NSC proliferation and neuronal differentiation in vitroand in the hippocampus and subventricular zone of adult rats, as compared to uncoated bulk curcumin. Cur-PLGA-NPs induce neurogenesis by internalization into the hippocampal NSC. Cur-PLGA-NPs significantly increase expression of genes involved in cell proliferation (reelin, nestin, and Pax6) and neuronal differentiation (neurogenin, neuroD1, neuregulin, neuroligin, and Stat3). Curcumin nanoparticles increase neuronal differentiation by activating the Wnt/β-catenin pathway, involved in regulation of neurogenesis. These nanoparticles caused enhanced nuclear translocation of β-catenin, decreased GSK-3β levels, and increased promoter activity of the TCF/LEF and cyclin-D1. Pharmacological and siRNA-mediated genetic inhibition of the Wnt pathway blocked neurogenesis-stimulating effects of curcumin. These nanoparticles reverse learning and memory impairments in an amyloid beta induced rat model of AD-like phenotypes, by inducing neurogenesis. In silicomolecular docking studies suggest that curcumin interacts with Wif-1, Dkk, and GSK-3β. These results suggest that curcumin nanoparticles induce adult neurogenesis through activation of the canonical Wnt/β-catenin pathway and may offer a therapeutic approach to treating neurodegenerative diseases such as AD, by enhancing a brain self-repair mechanism.

Published
2014
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