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Rodents are the primary hosts of Bartonella species and carry more than 22 Bartonella species. However, the information on epidemiological characteristics and genetic diversity of Bartonella species in rodents in southeastern China is limited. From 2015 to 2020, 1,137 rodents were captured. Bartonella-positive DNA was detected in 14.9% (169/1,137) of rodents by PCR for both the ssrA and gltA genes. A highest Bartonella prevalence was detected in Apodemus agrarius (33.5%) and lowest in B. indica (1.8%). The probability of Bartonella infection in summer (20.1%) was higher than in spring (14.6%; p = .011, OR = 1.756). Sequencing and phylogenetic analysis revealed that nine known Bartonella species were identified in rodents, including B. tribocorum, B. grahamii, B. rattimassiliensis, B. queenslandensis, B. elizabethae, B. phoceensis, B. coopersplainsensis, B. japonica and B. rochalimae. In our study, Bartonella species exhibited a strong association with their hosts. Zoonotic B. tribocorum, B. grahamii, B. elizabethae and B. rochalimae were found in synanthropic rodent species in southeastern China, which pose a potential threat to the public health. To prevent the spread of zoonotic Bartonella species to humans, preventive and control measures should be implemented, and more research is needed to confirm the pathogen's association with human disease.

Background: ISO 15189:2012 is widely recommended for clinical laboratories. However, it has not been paid enough attention in medical laboratory education and experiments in clinical biochemistry courses. Methods/Approach: The teaching program of clinical biochemistry aims to improve students’ awareness and ability of quality management according to ISO 15189 technical requirements. It is a 4-stage experimental training program to teaching skills: "establish an overall testing process based on the patient's clinical indicator, clarifying principles, improve operational skills, review process and continuous improvement". The program was implemented in our college during the winter semesters of 2019 and 2020. 185 students participate in the program as an experimental group, other 172 students as control group adopt the conventional method. Participants completed an online survey after each class to evaluate the class. Results/Outcomes: The test group had significantly better examination scores not only in experimental operational skills (89.27±7.16 vs. 77.51±4.72, pConclusions: A 4-stage experimental training program based on ISO 15189 technical requirements combined with CBL in clinical biochemistry is an effective and acceptable teaching strategy for the conventional training program.

Endothelial cell dysfunction plays a fundamental role in the pathogenesis of atherosclerosis (AS), and endothelial autophagy has protective effects on the development of AS. Our previous study had shown that oxidized low-density lipoprotein/β2-glycoprotein I/anti-β2-glycoprotein I antibody (oxLDL/β2GPI/anti-β2GPI) complex could promote the expressions of inflammatory cytokines and enhance the adhesion of leukocytes to endothelial cells. In the present study, we aimed to assess the effects of oxLDL/β2GPI/anti-β2GPI complex on endothelial autophagy and explore the associated potential mechanisms. Human umbilical vein endothelial cells (HUVECs) and mouse brain endothelial cell line (bEnd.3) were used as models of the vascular endothelial cells. Autophagy was evaluated by examining the expressions of autophagic proteins using western blotting analysis, autophagosome accumulation using transmission electron microscopy, and RFP-GFP-LC3 adenoviral transfection and autophagic flux using lysosome inhibitor chloroquine. The expressions of phospho-PI3K, phospho-AKT, phospho-mTOR, and phospho-eNOS were determined by western blotting analysis. 3-Methyladenine (3-MA) and rapamycin were used to determine the role of autophagy in oxLDL/β2GPI/anti-β2GPI complex-induced endothelial cell dysfunction. We showed that oxLDL/β2GPI/anti-β2GPI complex suppressed the autophagy, evidenced by an increase in p62 protein, a decrease in LC3-II and Beclin1, and a reduction of autophagosome generation in endothelial cells. Moreover, inhibition of autophagy was associated with PI3K/AKT/mTOR and eNOS signaling pathways. Rapamycin attenuated oxLDL/β2GPI/anti-β2GPI complex-induced endothelial inflammation, oxidative stress, and apoptosis, whereas 3-MA alone induced the endothelial injury. Our results suggested that oxLDL/β2GPI/anti-β2GPI complex inhibited endothelial autophagy via PI3K/AKT/mTOR and eNOS signaling pathways and further contributed to endothelial cell dysfunction. Collectively, our findings provided a novel mechanism for vascular endothelial injury in AS patients with an antiphospholipid syndrome (APS) background.

Traditional Chinese medicine recommends kidney-tonifying treatment for osteoporosis. Zuogui pill is one such treatment composed of eight well characterized Chinese medicinal herbs (Radix rehmanniae preparata, Fructus lycii, Rhizoma dioscoreae, F. corni, Semen cuscutae, Colla cornus cervi, C. carapacis et Plastri testudinis, and R. achyranthis bidentatae). The aim of the study is to evaluate the effects of the Zuogui pill on osteoporosis in ovariectomized rats and to identify the signaling pathways that mediate its action on the bones. A total of 46 6-month-old female Sprague-Dawley rats were divided into four groups: sham-operated control, bilaterally ovariectomized, ovariectomized rats receiving diethylstilbestrol (the positive control group), and ovariectomized rats receiving the Zuogui pill. By the end of 8-week treatments, the following parameters were assessed: (a) bone mineral density and trabecular histomorphology, (b) the expression levels of receptor activator of nuclear factor kappa B ligand and osteoprotegerin in rat tibial osteoblasts and (c) bone marrow stromal cells. The Zuogui pill treatment elevated the trabecular bone area and trabecular thickness and reduced the trabecular spacing in rats with ovariectomy-induced osteoporosis. Compared to the ovariectomized group, the Zuogui pill treatment significantly increased the levels of bone mineral density and osteoprotegerin and suppressed the level of receptor activator of the nuclear factor kappa B ligand. These data led us to conclude that the Zuogui pill regulates bone metabolism and improves osteoporosis symptoms possibly through the osteoprotegerin/receptor activator of the nuclear factor kappa B ligand/receptor activator of the nuclear factor kappa B signaling pathway.

TianWei Guo,1,* Wei Wang,2,* YueXia Ji,1 Min Zhang,3 GuoYing Xu,4 Sen Lin5 1Department of Pathology, Changshu Hospital Affiliated to Nanjing University of Chinese Medicine, Changshu, Jiangsu, People’s Republic of China; 2Department of Pathology, The Affiliated Obstetrics and Gynecology Hospital of Nanjing Medical University, Nanjing Maternity and Child Health Care Hospital, Nanjing, Jiangsu, People’s Republic of China; 3Department of Pathology, Children’s Hospital Affiliated to Soochow University, Suzhou, Jiangsu, People’s Republic of China; 4School of Medical Technology, Jiangsu College of Nursing, Huai’an, Jiangsu, People’s Republic of China; 5The Affiliated Huai’an Hospital of Xuzhou Medical University and the Second People’s Hospital of Huai’an, Huai’an, Jiangsu, People’s Republic of China*These authors contributed equally to this workCorrespondence: Sen LinThe Affiliated Huai’an Hospital of Xuzhou Medical University and the Second People’s Hospital of Huai’an, Huai’an, Jiangsu, People’s Republic of ChinaEmail linsen378@163.comGuoYing XuSchool of Medical Technology, Jiangsu College of Nursing, Huai’an, Jiangsu, People’s Republic of ChinaEmail xuguoying3@163.comIntroduction: Growing evidences imply that multiple long non-coding RNAs (lncRNAs) play a significant role in the treatment of cancer. Therefore, it is of great significance to discover new biomarkers or therapeutic targets of gastric cancer (GC). However, the potential molecular mechanism of lncPROX1-AS1 in GC remains unknown. The objective of current study is to investigate the effect of PROX1-AS1 in GC.Methods: Thus, we detect that PROX1-AS1 is over-expressed in tissues and cell lines of GC using qRT-PCR analysis. CCK-8, colony formation, flow cytometry, wounding healing and transwell analyses were performed to explore the effect of PROX1-AS1 on GC malignant behaviors.Results: It is further disclosed that silencing of PROX1-AS1 represses cell proliferation, migration, and invasion, whereas promotes cell apoptosis in GC. Bioinformatics analysis suggests that miR-877-5p is negatively regulated by PROX1-AS1 and ectopic of miR-877-5p alleviates the malignant behaviors of GC. Subsequently, miR-877-5p suppresses the activity of PD-L1-3ʹ UTR. At last, rescue assays demonstrated that the GC progression is suppressed by sh-PROX1-AS1 and facilitated on account of miR-877-5p inhibitors and then is retrieved by sh-PD-L1.Discussion: Our findings reveal that PROX1-AS1 exerts its role via miR-877-5p/PD-L1 axis in the GC progression, suggesting that PROX1-AS1 may represent a new therapeutic target for the diagnosis and treatment of GC patients.Keywords: PROX1-AS1, miR-877-5p, PD-L1, proliferation, apoptosis, gastric cancer

Background Leptospirosis is a significant emerging infectious disease worldwide. Rodents are considered to be the most critical hosts for Leptospira spp. Fujian Province is a region highly endemic for leptospirosis in China. However, the genetic diversity of leptospires circulating among rodents in Fujian is limited. Results The carrier status of Leptospira spp. in rats was investigated by culturing and serological detection in Fujian during 2018-2020. A total of 710 rodents, including 11 species, were trapped, with R. rattoides being the dominant species (50.56%). Fourteen pathogenic Leptospira strains were obtained. Two species including L. interrogans and L. borgpetersenii were identified using 16S rRNA gene. Four serogroups were identified by the microscopic agglutination test (MAT), including 7 Javanica, 4 Icterohaemorrhagiae, 2 Bataviae and 1 Pyrogenes. Six STs were identified by MLST, including 7 ST143, 2 ST1, 2 ST17, 1 ST96, 1 ST332 and 1 ST333. L. borgpetersenii serogroup Javanica belonging to ST143 was the dominant type (50.00%). A total of 387 rat serum samples were tested by MAT. Sera were considered positive for seroreactivity at a titer ≥1:20 against at least one serovar. A total of 138 (35.66%) sera samples tested positive, and seven serogroups were identified, with Javanica being the dominant serogroup (81.16%), the similar serogroup isolated from rodents. This study demonstrates a high prevalence of leptospirosis in rats and public health education among high-risk workers is highly recommended. Conclusions This study confirmed R. rattoides were the most abundantly rats, and L. borgpetersenii serogroup Javanica ST143, L. interrogans serogroup Icterohaemorrhagiae ST1 and ST17 were widely distributed among rodents in Fujian during 2018-2020. Despite the low number of isolates obtained from rodents, this study suggests that continuous epidemiological surveillance on the etiological characteristics of pathogenic Leptospira in wild animal reservoirs may help reduce the possible risk of disease transmission.

RNA, one of the major building blocks of the cell, participates in many essential life processes. RNA stability is well-established to be closely related to various RNA modifications. To date, hundreds of different RNA modifications have been identified. N6-methyladenosine (m

Background: Babesiosis is a tick-borne disease that mainly affects small mammals and it has been reported in at least five provinces in China. However, the host range and geographical distribution of the parasite in Fujian Province are unclear. Therefore, this study aimed to investigate the prevalence and genetic characteristics of Babesia in Fujian Province, Southeast China, between 2015 and 2020.Methods: Rodent blood samples were collected from 26 different surveillance sites across Fujian Province. Genomic DNA was extracted to screen for Babesia infection using polymerase chain reaction (PCR) amplification, based on 18S rRNA. DNA samples from 316 domestic goats, 85 water buffalo, 56 domestic dogs, and 18 domestic pigs were examined. The prevalence of Babesia was statistically analyzed using the Chi-square test or Fisher’s exact test. A phylogenetic tree was constructed using MEGA 5.0 by gene sequence alignment.Results: Babesia infections were found in 3.96% (43/1,087) of rodents and 1.26% (6/475) of other mammals. Multivariate logistic regression analysis revealed that irrigated cropland, shrubs, and forests were risk factors for Babesia microti infections. The infection rates among domestic pigs, dogs, and goats were found to be 5.56%, 1.79%, and 1.27%, respectively, and no infection was found in water buffalo. Sequencing (18S rRNA gene) revealed that rodents were infected with Babesia (sensu lato) while other mammals were infected with Babesia (sensu stricto).Conclusions: The results indicate that there is wide geographical distribution and phylogenetic relationship of Babesia in Southeast China. This study suggests that mammals, especially wild rodents, are the main natural hosts of Babesia in Fujian. Our findings provide a solid foundation for public health officials to develop Babesia prevention and control measures.

Background: Babesiosis is a tick-borne infectious disease of socio-economic importance. The clinical manifestations of babesiosis are usually intermittent fever, headache, chills, sweats, and myalgia. Babesia infections in small mammals and ticks have been reported in at least five provinces in China. However, the host range and geographical distribution of this parasite in Fujian Province are unclear. The aim of this study was to investigate the prevalence and genetic characteristics of Babesia in Fujian Province, Southeast China, between 2015 and 2020. Methods: Rodent blood samples were collected from 26 different surveillance sites across Fujian Province. Genomic DNA was extracted to screen for Babesia infection via PCR amplification based on 18S rRNA. The prevalence of Babesia was compared using the chi-square test or Fisher’s exact test. A phylogenetic tree was constructed using MEGA 5.0 by gene sequence alignment. DNA samples from 316 domestic goats, 85 water buffalo, 56 domestic dogs, and 18 domestic pigs were also collected in this survey.Results: Babesia infection was confirmed in 3.96% (43/1087) of rodents and in 1.26% (6/475) of other mammals. Multivariate logistic regression analysis revealed that irrigated cropland, shrub, and forest were risk factors for Babesia microti infection. The infection rates of domestic pigs, dogs, and goats were determined to be 5.56%, 1.79%, and 1.27%, respectively, and no infection was found in water buffalo. Sequencing using the 18S rRNA gene revealed that rodents were infected with Babesia (sensu lato) while other mammals were infected with Babesia (sensu stricto). Conclusions: The results indicate that there is a broad geographical distribution and phylogenetic relationship of Babesia in Southeast China. This study suggests that mammals, especially wild rodents, are the main natural hosts of Babesia in Fujian. Our research provides new insights into the exposure risk of Babesia in humans and animals, laying a solid foundation for the development of babesiosis prevention and control measures.

1066 Background: Dalpiciclib (Dalp; SHR6390) is a novel cyclin-dependent kinase 4/6 inhibitor which showed tolerability and preliminary clinical activity as monotherapy for pretreated advanced breast cancer (ABC). Here we conducted a multicenter, phase 1b trial to further assess the safety, pharmacokinetics and efficacy of Dalp in combination with endocrine therapy (ET) in HR+/HER2- ABC. Methods: 5 cohorts of patients with HR+/HER2- locally recurrent or metastatic BC and any menopausal status were enrolled (~15 patients/combination regimen). Patients without prior treatment for ABC (cohort1/2) were given Dalp (125 or 150 mg po qd, d1-21, q4w) plus letrozole (LTZ; 2.5 mg po qd) or anastrozole (ATZ; 1 mg po qd); patients who progressed after ET (cohort 3-5) were given Dalp (125, 150, or 175 mg po qd, d1-21, q4w) plus fulvestrant (Fulv; 500 mg im, cycle 1 d1, d15, then d1 q4w). The primary endpoint was safety. The data cutoff date was Sep. 20, 2021. Results: 58 patients received Dalp plus LTZ/ATZ and 46 received Dalp plus Fulv. No maximum tolerated dose of Dalp was reached with LTZ/ATZ or Fulv. Across all cohorts, 75.0%-93.8% of patients had a grade ≥3 treatment-related adverse event (TRAE), with the most common being neutropenia (grade 3, 40.0%-87.5%; grade 4, 4.2%-46.7%) and leukopenia (grade 3, 33.3%-80.0%; grade 4, 0%; Table). Treatment-related serious AEs occurred in 2 (3.4%) patients with Dalp plus LTZ/ATZ and none with Dalp plus Fulv. At the tested dose levels, steady-state areas under the concentration curve and peak concentration of Dalp increased with dose in combination with LTZ/ATZ or Fulv. Dalp 150 mg was associated with a numerically higher objective response rate in both ET-untreated (67.6%, 95% CI 49.5-82.6) and ET-pretreated (53.3%, 95% CI 26.6-78.7) patients per investigator. The median progression-free survival with Dalp 150 mg was 20.3 mo (95% CI 16.9-not reached [NR]) and 16.7 mo (95% CI 1.9-24.1) in ET-untreated and ET-pretreated patients respectively. Conclusions: Dalpiciclib plus letrozole/anastrozole or fulvestrant showed an acceptable safety profile, with hematological toxicities as the most common TRAEs. The recommended phase 3 dose of dalpiciclib was 150 mg. Together with the promising anti-tumor activity observed with the combination therapy in HR+/HER2- ABC, further trials are warranted. Clinical trial information: NCT03481998. [Table: see text]

Objective This study aimed to investigate the expression of long non-coding RNA (lncRNA) growth arrest-special transcript 5 (GAS5) in the serum of tuberculosis (TB) patients and discuss the mechanism of GAS5 in TB by establishing an in-vitro TB cell model. Methods Serum expressions of GAS5 and miR-18a-5p were determined by quantitative real-time PCR (qRT-PCR). The effects of GAS5 on macrophage cell viability and the inflammatory response after MTB infection were assessed by CCK-8 and ELISA. Luciferase reporter gene assay was applied to delve into the potential target gene of GAS5. Results The expression of GAS5 in TB patients was down-regulated, while miR-18a-5p was up-regulated, and the serum inflammatory factors were negatively correlated with the expression level of GAS5. MTB infection induced significant upregulation on the cell viability and inflammatory response but the acceleration effect could be rescued by GAS5-overexpression. Meanwhile, miR-18a-5p was recognized as the target gene of GAS5. Conclusion This study indicated that the expression level of GAS5 in the serum of TB patients was decreased, while in the cells infected with MTB, the down-regulated GAS5 might develop a role in facilitating the cell vitality and the inflammatory response by adsorbing miR-18a-5p in the form of molecular sponge.

Endothelial cell dysfunction plays a fundamental role in the pathogenesis of atherosclerosis (AS), and endothelial autophagy has protective effects on the development of AS. Our previous study had shown that oxidized low-density lipoprotein/β2-glycoprotein I/anti-β2-glycoprotein I antibody (oxLDL/β2GPI/anti-β2GPI) complex could promote the expressions of inflammatory cytokines and enhance the adhesion of leukocytes to endothelial cells. In the present study, we aimed to assess the effects of oxLDL/β2GPI/anti-β2GPI complex on endothelial autophagy and explore the associated potential mechanisms. Human umbilical vein endothelial cells (HUVECs) and mouse brain endothelial cell line (bEnd.3) were used as models of the vascular endothelial cells. Autophagy was evaluated by examining the expressions of autophagic proteins using western blotting analysis, autophagosome accumulation using transmission electron microscopy, and RFP-GFP-LC3 adenoviral transfection and autophagic flux using lysosome inhibitor chloroquine. The expressions of phospho-PI3K, phospho-AKT, phospho-mTOR, and phospho-eNOS were determined by western blotting analysis. 3-Methyladenine (3-MA) and rapamycin were used to determine the role of autophagy in oxLDL/β2GPI/anti-β2GPI complex-induced endothelial cell dysfunction. We showed that oxLDL/β2GPI/anti-β2GPI complex suppressed the autophagy, evidenced by an increase in p62 protein, a decrease in LC3-II and Beclin1, and a reduction of autophagosome generation in endothelial cells. Moreover, inhibition of autophagy was associated with PI3K/AKT/mTOR and eNOS signaling pathways. Rapamycin attenuated oxLDL/β2GPI/anti-β2GPI complex-induced endothelial inflammation, oxidative stress, and apoptosis, whereas 3-MA alone induced the endothelial injury. Our results suggested that oxLDL/β2GPI/anti-β2GPI complex inhibited endothelial autophagy via PI3K/AKT/mTOR and eNOS signaling pathways and further contributed to endothelial cell dysfunction. Collectively, our findings provided a novel mechanism for vascular endothelial injury in AS patients with an antiphospholipid syndrome (APS) background.

Background Bone homeostasis is a tightly orchestrated process maintained by osteoblasts and osteoclasts, and a disruption of their steady-state equilibrium can lead to the occurrence of osteoporosis (OP). Methods We investigated the differential expression of micro (mi)RNAs in the bone tissues of a postmenopausal osteoporosis rat model induced by ovariectomy (OVX). Real-time PCR was used to verify the differentially expressed miRNAs in bone samples from OP patients and controls. The specific targets of two differentially expressed miRNAs in osteogenic or osteoclast differentiation were determined by bioinformatic prediction, and mRNA and protein detection. Results miR-708-5p and miR-708-3p were highly expressed in the bone tissue of OVX rats and OP patients. miR-708-5p negatively regulated osteoblast differentiation in bone marrow mesenchymal stem cells by targeting SMAD specific E3 ubiquitin protein ligase 2, while miR-708-3p positively regulated osteoclast differentiation in bone marrow monocytes by targeting cerebellar degeneration associated protein 1 antisense RNA. miR-708-5p and miR-708-3p were shown to originate from the same precursor miRNA and to have a synergistic effect on the development of osteoporosis with different temporal and spatial patterns. Conclusion Our findings provide a referential theoretical basis and targets for the prevention and treatment of osteoporosis.

Immunotherapy is the most promising new cancer treatment for various pediatric tumors and has resulted in an unprecedented surge in the number of novel immunotherapeutic treatments that need to be evaluated in clinical trials. Most phase I/II trial designs have been developed for evaluating only one candidate treatment at a time, and are thus not optimal for this task. To address these issues, we propose a Bayesian phase I/II platform trial design, which accounts for the unique features of immunotherapy, thereby allowing investigators to continuously screen a large number of immunotherapeutic treatments in an efficient and seamless manner. The elicited numerical utility is adopted to account for the risk-benefit trade-off and to quantify the desirability of the dose. During the trial, inefficacious or overly toxic treatments are adaptively dropped from the trial and the promising treatments are graduated from the trial to the next stage of development. Once an experimental treatment is dropped or graduated, the next available new treatment can be immediately added and tested. Extensive simulation studies have demonstrated the desirable operating characteristics of the proposed design.

Energy crisis and environment damage are the two worldwide urgent problems and building energy plays an important role in the energy and environment field. To reduce heating and cooling energy consumption in buildings, a novel solar assisted hybrid power gas heat pump system is proposed. The novel system runs in five working conditions and applies solar energy combined with battery pack to guarantee the engine always works in its economic zones. Because of the complexity of photovoltaic panels in environment effect, the life cycle assessment method is introduced to evaluate the whole system’s energy and environment effect. According to different photovoltaic ratios, simulation shows that the application of solar panels achieves a significant improvement in energy consumption while also causes a large mount in environment damage. At last the environmental payback time is put forward and the best environment payback time for the photovoltaic hybrid power gas heat pump system is 13.4 years at a photovoltaic ratio of 40%.

Spray flash evaporation (SFE) technology is a promising way for efficient low-temperature desalination that can utilize waste heat or solar energy as heat source. In this paper, a solar driven-SFE desalination system enhanced by latent heat fluids is presented. The NaCl aqueous solution with addition of microcapsulated phase change material (MPCM) acts as the working fluid for thermal energy storage and the SFE process. Based on the physical properties measurements of the MPCM slurry, the flash evaporation characteristics of sprayed droplets were simulated and performances of the SFE desalination system were evaluated. The simulative results reveals that the temperature drop of droplets with MPCM may slow down during flash evaporation process, due to their higher effective heat capacity. Hence, by adding MPCM with mass fraction of 0.18 into the flash evaporation solution, the water production ratio could be increased by 23.1 %, and the unit energy consumption of the system reduced by 18.3 %. Furthermore, the effects of operating pressure the chamber and inlet superheat temperature on the SFE desalination system enhanced by MPCM were discussed, providing guidance for optimum design.

Building energy consumption accounts for a large proportion in the total social energy consumption and solar energy plays an important role in the building renewable energy system. A novel hybrid power gas heat pump system integrated with photovoltaic is put forward in this paper. An optimization design based on life cycle assessment for the solar assisted hybrid power gas heat pump system (SHPGHP) is proposed in consideration of three main environmental impact factors: human health, ecosystem quality and resources. The normalized environmental impact for the system during the whole life cycle is chosen as the objective to minimize the life cycle environmental impact. The influence of main independent decision variables is employed including the photovoltaic ratio, transmission ratio and mixing degree. Results show that the optimal photovoltaic ratio is 0.4. The system's environmental impact potential increases with the increase of transmission ratio and decreases with the increase of mixing degree.

BACKGROUND Eph receptors comprise the largest group of the receptor tyrosine kinase (RTK) family, and Eph receptors interacting with their ligand ephrins play an important role in development and tumorigenesis. EphB6, a special Eph receptor that lacks tyrosine kinase activity, was reported to be expressed in some human cancers. The clinical significance of EphB6 in gastric carcinoma has not been well investigated. METHODS In this study, we detected expression of EphB6 protein in four gastric cancer cell lines and a set of gastric carcinoma tissue specimens by using immunohistochemistry. The relationship between EphB6 protein expression and clinicopathological parameters was statistically analyzed. RESULTS EphB6 protein was differentially detected in four gastric cancer cell lines. EphB6 protein was low expressed in 52.6%, moderately expressed in 32.59%, and strongly expressed in 14.5% of gastric carcinomas. EphB6 expression was positively associated with tumor differentiation (P< 0.001, rs= 0.476), and negatively associated with lymph node metastasis (P< 0.001, rs=-0.444) and tumor stage (P= 0.001, rs=-0.269). Low EphB6 expression was detected more often in female patients (P= 0.031). No significant relationship between EphB6 expression and patient age, tumor location, or depth of tumor invasion was identified. CONCLUSION Our data indicate that EphB6 protein was decreased in gastric carcinoma compared with normal mucosa. Analytic results based on pathological parameters suggests that EphB6 protein may inhibit metastasis of gastric carcinoma and could be a potential therapeutic target for gastric carcinoma.