369 results on '"Guodong Gao"'
Search Results
2. Maintaining Drosha expression with Cdk5 inhibitors as a potential therapeutic strategy for early intervention after TBI
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Lu Huang, Li Xia, Tiejian Nie, Bozhou Cui, Jianjun Lu, Fangfang Lu, Feiyan Fan, Dongni Ren, Yuan Lu, Guodong Gao, and Qian Yang
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Medicine ,Biochemistry ,QD415-436 - Abstract
Abstract Traumatic brain injury (TBI) is a major cause of death and disability in adults. The pathological process of TBI involves a multifactorial cascade in which kinases have been proven contribute to interactions between relevant factors and amplification of signaling cascades. Cyclin-dependent kinase 5 (Cdk5) is a promising kinase that has been implicated in various brain disorders, including TBI. However, the mechanism by which Cdk5 induces neuronal damage remains unclear. Here, we show for the first time that Drosha, a key enzyme in microRNA biogenesis, is a pivotal substrate of abnormally activated Cdk5. Cdk5-mediated phosphorylation decreases Drosha expression and exacerbates nerve injury in TBI. We proved that maintaining Drosha expression via the administration of repurposed Cdk5 inhibitors that were previously studied in clinical trials is a promising approach for the early treatment of TBI. Together, our work identifies Drosha as a novel target for neuroprotective strategies after TBI and suggests Cdk5-mediated regulation of Drosha expression as a potential therapeutic strategy for early TBI intervention.
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- 2024
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3. FGF21 alleviates endothelial mitochondrial damage and prevents BBB from disruption after intracranial hemorrhage through a mechanism involving SIRT6
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Runfeng Wang, Jin Wang, Zhiguo Zhang, Bo Ma, Shukai Sun, Li Gao, and Guodong Gao
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FGF21 ,SIRT6 ,Endothelial cells ,BBB ,Mitochondria quality control ,ICH ,Therapeutics. Pharmacology ,RM1-950 ,Biochemistry ,QD415-436 - Abstract
Abstract Background Disruption of the BBB is a harmful event after intracranial hemorrhage (ICH), and this disruption contributes to a series of secondary injuries. We hypothesized that FGF21 may have protective effects after intracranial hemorrhage (ICH) and investigated possible underlying molecular mechanisms. Methods Blood samples of ICH patients were collected to determine the relationship between the serum level of FGF21 and the $$\Delta$$ Δ GCS%. Wild-type mice, SIRT6flox/flox mice, endothelial-specific SIRT6-homozygous-knockout mice (eSIRT6−/− mice) and cultured human brain microvascular endothelial cells (HCMECs) were used to determine the protective effects of FGF21 on the BBB. Results We obtained original clinical evidence from patient data identifying a positive correlation between the serum level of FGF21 and $$\Delta$$ Δ GCS%. In mice, we found that FGF21 treatment is capable of alleviating BBB damage, mitigating brain edema, reducing lesion volume and improving neurofunction after ICH. In vitro, after oxyhemoglobin injury, we further explored the protective effects of FGF21 on endothelial cells (ECs), which are a significant component of the BBB. Mitochondria play crucial roles during various types of stress reactions. FGF21 significantly improved mitochondrial biology and function in ECs, as evidenced by alleviated mitochondrial morphology damage, reduced ROS accumulation, and restored ATP production. Moreover, we found that the crucial regulatory mitochondrial factor deacylase sirtuin 6 (SIRT6) played an irreplaceable role in the effects of FGF21. Using endothelial-specific SIRT6-knockout mice, we found that SIRT6 deficiency largely diminished these neuroprotective effects of FGF21. Then, we revealed that FGF21 might promote the expression of SIRT6 via the AMPK–Foxo3a pathway. Conclusions We provide the first evidence that FGF21 is capable of protecting the BBB after ICH by improving SIRT6-mediated mitochondrial homeostasis.
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- 2023
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4. Vitamin C Alleviates Intestinal Inflammation Caused by Aeromonas hydrophila in Juvenile Blunt Snout Bream (Megalobrama amblycephala)
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Abdullateef Mukhtar Muhammad, Chang Yang, Jingyuan Wang, Xianping Ge, Bo Liu, Linghong Miao, Guodong Gao, and Qunlan Zhou
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VC ,immune responses ,antioxidant enzyme ,cytokines ,intestinal inflammation ,NF-kB ,Biology (General) ,QH301-705.5 ,Genetics ,QH426-470 - Abstract
Vitamin C (VC) can be used to increase disease resistance in practice in intensive aquaculture. But it is still unconfirmed whether VC could alleviate inflammation and what dosage is suitable. This study investigated the effects of dietary VC on the immunity and enteritis of juvenile blunt snout bream (Megalobrama amblycephala) challenged with Aeromonas hydrophila. The fish were fed with VC levels ranging from 25.35 to 2231.98 mg/kg for 60 days. After that, fish fed with 150.65 mg/kg, 573.79 mg/kg, and 2231.98 mg/kg VC were challenged with A. hydrophila orally to simulate enteritis. The results showed that 285.39 and 573.79 mg/kg dietary VC significant improved growth performance and feed utilization. Dietary VC (573.79 and 1133.79 mg/kg) significantly enhanced the serum immune parameters, antioxidant enzymes’ activities, and relative gene expressions of ikbα and cat in the liver in the 60-day feeding trail. The survival rate was significantly higher in 573.79 mg/kg dietary VC group than the other two treatments. Cytokines were activated after the bacteria challenge. Dietary 573.79 mg/kg VC caused significantly higher TNF-α level at 12 hpi and maintained a high level of IL-8 at 48 to 72 h compared to other treatments. In conclusion, more than 500 mg/kg VC was needed to maintain the health of blunt snout bream juveniles. Suitable VC would activate cytokines to increase disease resistance along with antioxidant enzymes.
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- 2024
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5. Preparation of antiadhesion polypropylene mesh using bacterial cellulose combined with chitosan hydrogel application in rat abdominal incisional hernia
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Zicheng Guo, Fangjie Zhang, Hao Wu, Guodong Gao, Kangbei Zhu, Yaqin Qi, and Mingliang He
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antiadhesion mesh ,bacterial cellulose ,chitosan ,incisional hernia ,Surgery ,RD1-811 - Abstract
OBJECTIVE: To develop an antiadhesion polypropylene (PP) mesh by incorporating bacterial cellulose (BC) combined with chitosan (CS) hydrogel and to evaluate its properties using an intraperitoneal mesh repair approach in a rat model of abdominal incisional hernia. MATERIALS AND METHODS: BC was used to prepare hydrogels through cross-linking of CS. The hydrogel was adhered to the surface of a PP mesh using a glue applicator to form an antiadhesion mesh. Forty-eight male rats were randomly divided into four groups of 12 members each: PP, antiadhesion mesh (Sepramesh), BC mesh, and BCCS mesh. Incisional hernia models of the abdominal wall of the rats were created sequentially, and meshes were subsequently inserted. At 7 days postoperation, six rats were randomly selected for euthanasia, and the repaired abdominal wall tissues, adhesions, and mesh were removed for pathological analysis. Similarly, the remaining six rats were euthanized at 14 days postoperation, and their respective abdominal wall tissues, adhesions, and mesh were also excised for pathological examination. RESULTS: BC is combined with CS hydrogel, and the mixture is adhered to the PP mesh using a glue applicator. The mesh adhesion scores in the PP group were consistently high. The adhesion scores of the BC group were better than those of the PP group. There were no statistically significant differences in the indices between the Sepramesh and BCCS groups, and both groups showed lower adhesion grades. Hematoxylin-eosin staining revealed that the natural tissue inflammatory response of the BCCS group was lower, and its prosthetic (mesh) surface inflammatory response was lower than that of the other groups. On the other hand, the inflammatory response in the BCCS group was lower. Masson’s trichrome staining showed that the number of cells formed in the scar plate of the Sepramesh and BCCS groups was significantly lower than that of the other groups, the degree of tissue ingrowth was also better in both groups, and the biocompatibility of the BCCS group was better. CONCLUSION: BC is bonded with CS hydrogel and then adhered to the surface of PP mesh by an applicator to make an antiadhesive mesh. This mesh may reduce intraabdominal adhesions and intraabdominal bacterial colonization while possessing good strength and biocompatibility, thereby contributing to peritoneal repair.
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- 2023
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6. Retraction Note: Single-wall carbon nanohorns inhibited activation of microglia induced by lipopolysaccharide through blocking of Sirt3
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Lihong Li, Jinqian Zhang, Yang Yang, Qiang Wang, Li Gao, Yanlong Yang, Tao Chang, Xingye Zhang, Guoan Xiang, Yongmei Cao, Zujin Shi, Ming Zhao, and Guodong Gao
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Materials of engineering and construction. Mechanics of materials ,TA401-492 - Published
- 2023
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7. Reducing CSF complications by a recycled Hadad’s flap combined with autologous mucosa in secondary endoscope transsphenoidal surgery
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Runfeng Wang, Gaoyang Zhou, Jin Wang, Bo Ma, Ping Wang, Guodong Gao, Shukai Sun, and Zhiguo Zhang
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repeated transsphenoidal surgery ,Hadad-Bassagasteguy flap ,CSF complications ,skull base surgery ,transsphenoid approach ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
BackgroundTranssphenoidal secondary operations are a minority but not a rare occurrence. How to viably prevent cerebral fluid (CSF)-related complications and confine surgery-caused injury in secondary surgery as minimally as possible is a huge challenge. This article shares our solution of recycling a prior Hadad-Bassagasteguy flap (HBF) along with a using small piece of free autologous mucosa to reconstruct the skull base.MethodsOf 69 patients, fitted criteria were assigned into 2 different groups: a recycled HBF incorporated with an autologous free mucosa and a recycled HBF incorporated with an artificial dura to rebuild the skull base in secondary transsphenoidal surgery. The postoperative morbidities of pseudomeningocele, CSF leakage and meningitis were recorded and analyzed.ResultsA recycled HBF incorporated with an autologous mucosa is capable of reducing CSF complications compared to that of the matched group, particularly decreasing the morbidity of meningitis in secondary transsphenoidal surgery. Diabetes mellitus, craniopharyngioma, chordoma and the utilization of artificial dura were independent risk factors for CSF complications in secondary transsphenoidal surgery through univariate and multivariate logistic regression. In addition, diabetes mellitus and artificial dura are more likely to induce CSF leakage and meningitis. Patients suffering from craniopharyngioma are more susceptible to meningitis. Chordoma indiscriminately increased the risk of each CSF complication.ConclusionA recycled HBF incorporated with an autologous mucosa is reliable for reconstructing the skull base in secondary transsphenoidal surgery, especially for patients simultaneously suffering from diabetes mellitus and central skull base tumors.
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- 2023
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8. Impact of hyperbilirubinemia associated acute kidney injury on chronic kidney disease after aortic arch surgery: a retrospective study with follow-up of 1-year
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Lin Lyu, Haicheng Song, Guodong Gao, He Dong, Pingping Liao, Ziying Shen, Hui Liu, Haichen Chu, and Li Yuan
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Hyperbilirubinemia ,Acute kidney injury ,Chronic kidney disease ,Aortic arch surgery ,Surgery ,RD1-811 ,Anesthesiology ,RD78.3-87.3 - Abstract
Abstract Background Hyperbilirubinemia (HB) is a serious complication in aortic arch surgery, which is associated with acute kidney injury (AKI). The association between HB and chronic kidney disease (CKD) is unknown. The aim of this study was to investigate the impact of HB associated AKI on CKD after aortic arch surgery. Methods We reviewed 284 patients who underwent aortic arch surgery from 2016 to 2020 in our hospital. AKI was defined as a 50% increase in sCr from baseline value within the first 7 postoperative days. HB was defined as total bilirubin > 51.3 μmol/L. Patients were divided into 3 groups based on AKI and HB: HB associated AKI (HB-AKI) group (AKI patients suffered HB within the first 7 postoperative days); AKI without HB group and Non-AKI group. Results Follow-up for 204 patients ranged from 3 to 12 months. Kaplan–Meier analysis showed that the 1-year cumulative incidence of CKD was highest in HB-AKI (32.6%) than AKI without HB (17.8%) and Non-AKI (7.4%, log-rank test, p
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- 2022
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9. Dietary supplementation with fermented antarctic krill shell improved the growth performance, digestive and antioxidant capability of Macrobrachium nipponense
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Ying Yan, Yan Lin, Lin Zhang, Guodong Gao, Shiyou Chen, Changhong Chi, Songqin Hu, Yuhang Sang, Xiaoyu Chu, Qunlan Zhou, Bo Liu, Yongfeng Zhao, Linghong Miao, and Xianping Ge
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Macrobrachium nipponense ,Fermented antarctic krill shell ,Growth performance ,Antioxidation ,Digestive enzyme ,Aquaculture. Fisheries. Angling ,SH1-691 - Abstract
To investigate the effects of different levels of fermented antarctic krill shell (FAKS) on the growth performance, hemolymph biochemistry, hepatopancreatic antioxidant capacity, and digestive enzyme activities of Macrobrachium nipponense, five isonitrogenous and isoenergetic experimental diets containing different proportions of FAKS (0 %, 0.25 %, 0.5 %, 1.0 %, 2.0 %) were fed to M. nipponense for 8 weeks. The results showed the weight gain rate (WGR), specific growth rate (SGR), head-to-body ratio (H/S), and head-to-tail ratio (H/T) increased first and then decreased with higher proportions of FAKS. The FAKS1.0 group exhibited the highest values, and significantly higher than that of the FAKS0 group (P
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- 2023
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10. The clinical and neurocognitive functional changes with awake brain mapping for gliomas invading eloquent areas: Institutional experience and the utility of The Montreal Cognitive Assessment
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Yuan Wang, Shaochun Guo, Na Wang, Jinghui Liu, Fan Chen, Yulong Zhai, Yue Wang, Yang Jiao, Wenjian Zhao, Chao Fan, Yanrong Xue, GuoDong Gao, Peigang Ji, and Liang Wang
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glioma ,awake brain mapping ,extent of resection (EOR) ,Karnofsky Performance Status (KPS) ,neurocognitive status ,Montreal Cognitive Assessment (MoCA) ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
ObjectiveAwake craniotomy with intraoperative brain functional mapping effectively reduces the potential risk of neurological deficits in patients with glioma invading the eloquent areas. However, glioma patients frequently present with impaired neurocognitive function. The present study aimed to investigate the neurocognitive and functional outcomes of glioma patients after awake brain mapping and assess the experience of a tertiary neurosurgical center in China over eight years.MethodsThis retrospective study included 80 patients who underwent awake brain mapping for gliomas invading the eloquent cortex between January 2013 and December 2021. Clinical and surgical factors, such as the extent of resection (EOR), perioperative Karnofsky Performance Score (KPS), progression-free survival (PFS), and overall survival (OS), were evaluated. We also used the Montreal Cognitive Assessment (MoCA) to assess the neurocognitive status changes.ResultsThe most frequently observed location of glioma was the frontal lobe (33/80, 41.25%), whereas the tumor primarily invaded the language-related cortex (36/80, 45%). Most patients had supratotal resection (11/80, 13.75%) and total resection (45/80, 56.25%). The median PFS was 43.2 months, and the median OS was 48.9 months in our cohort. The transient (less than seven days) neurological deficit rate was 17.5%, whereas the rate of persistent deficit (lasting for three months) was 15%. At three months of follow-up, most patients (72/80, 90%) had KPS scores > 80. Meanwhile, compared to the preoperative baseline tests, the changes in MoCA scores presented significant improvements at discharge and three months follow-up tests.ConclusionAwake brain mapping is a feasible and safe method for treating glioma invading the eloquent cortex, with the benefit of minimizing neurological deficits, increasing EOR, and extending survival time. The results of MoCA test indicated that brain mapping plays a critical role in preserving neurocognitive function during tumor resection.
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- 2023
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11. Refined imaging features of culprit plaques improve the prediction of recurrence in intracranial atherosclerotic stroke within the middle cerebral artery territory
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Guanmin Quan, Xuelian Wang, Yawu Liu, Lijuan Gao, Guodong Gao, Guojun Tan, and Tao Yuan
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Ischemic stroke ,Recurrence ,Middle cerebral artery ,Magnetic resonance imaging ,Vessel wall ,Computer applications to medicine. Medical informatics ,R858-859.7 ,Neurology. Diseases of the nervous system ,RC346-429 - Abstract
Recurrence is a significant adverse outcome of ischemic stroke (IS), particularly in cases of intracranial arteriosclerosis (ICAS). In this study, we investigated the impact of imaging features of culprit plaque using high-resolution magnetic resonance vessel wall imaging (HR-MR-VWI) on the prediction of IS recurrence. A total of 86 patients diagnosed with ICAS-related IS within the middle cerebral artery (MCA) territory were included, of which 23.25% experienced recurrent IS within one year. Our findings revealed significant differences between the recurrence and non-recurrence groups in terms of age (p = 0.007), diabetes mellitus (p = 0.031), hyperhomocysteinemia (p = 0.021), artery-artery embolism (AAE) infarction (p = 0.019), prominent enhancement (p = 0.013), and surface irregularity of the culprit plaque (p = 0.009). Age (HR = 1.063, p = 0.005), AAE infarction (HR = 5.708, p = 0.008), and prominent enhancement of the culprit plaque (HR = 4.105, p = 0.025) were identified as independent risk factors for stroke recurrence. The areas under the receiver operating characteristic curve (AUCs) for predicting IS recurrence using clinical factors, conventional imaging findings, HR-MR-VWI plaque features, and a combination of clinical and conventional imaging models were 0.728, 0.645, 0.705, and 0.814, respectively. Notably, the combination model demonstrated superior predictive performance with an AUC of 0.870. Similarly, AUC of combination model for predicting IS recurrence in validation cohort which enrolled another 37 patients was 0.865. In conclusion, the presence of obvious enhancement in culprit plaque on HR-MR-VWI is a valuable factor in predicting IS recurrence in ICAS-related strokes within the MCA territory. Furthermore, our combination model, incorporating plaque features, exhibited improved prediction accuracy.
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- 2023
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12. Relative T2-FLAIR signal intensity surrounding residual cavity is associated with survival prognosis in patients with lower-grade gliomas
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Tao Yuan, Zhen Gao, Fei Wang, Jia-Liang Ren, Tianda Wang, Hongbo Zhong, Guodong Gao, and Guanmin Quan
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lower-grade glioma ,survival ,prediction ,magnetic resonance imaging ,FLAIR = fluid-attenuated inversion recovery ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
AimsTo investigate whether the relative signal intensity surrounding the residual cavity on T2-fluid-attenuated inversion recovery (rFLAIR) can improve the survival prediction of lower-grade glioma (LGG) patients.MethodsClinical and pathological data and the follow-up MR imaging of 144 patients with LGG were analyzed. We calculated rFLAIR with Image J software. Logistic analysis was used to explore the significant impact factors on progression-free survival (PFS) and overall survival (OS). Several models were set up to predict the survival prognosis of LGG.ResultsA higher rFLAIR [1.81 (0.83)] [median (IQR)] of non-enhancing regions surrounding the residual cavity was detected in the progressed group (n=77) than that [1.55 (0.33)] [median (IQR)] of the not-progressed group (n = 67) (P1.622) were independent predictors for poor PFS (P1.622) in non-enhancing regions surrounding the residual cavity can be used as a biomarker of the poor survival of LGG. rFLAIR is helpful to improve the survival prediction of posttreatment LGG patients.
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- 2022
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13. Development of a nomogram to predict 30-day mortality of patients with sepsis-associated encephalopathy: a retrospective cohort study
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Yang Yang, Shengru Liang, Jie Geng, Qiuhe Wang, Pan Wang, Yuan Cao, Rong Li, Guodong Gao, and Lihong Li
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Sepsis ,Sepsis-associated encephalopathy ,30-day mortality ,Nomogram ,RDW ,Medical emergencies. Critical care. Intensive care. First aid ,RC86-88.9 - Abstract
Abstract Background Sepsis-associated encephalopathy (SAE) is related to increased short-term mortality in patients with sepsis. We aim to establish a user-friendly nomogram for individual prediction of 30-day risk of mortality in patients with SAE. Methods Data were retrospectively retrieved from the Medical Information Mart for Intensive Care (MIMIC III) open source clinical database. SAE was defined by Glasgow Coma Score (GCS) < 15 or delirium at the presence of sepsis. Prediction model with a nomogram was constructed in the training set by logistic regression analysis and then undergone internal validation and sensitivity analysis. Results SAE accounted for about 50% in patients with sepsis and was independently associated with the 30-day mortality of sepsis. Variables eligible for the nomogram included patient’s age and clinical parameters on the first day of ICU admission including the GCS score, lactate, bilirubin, red blood cell distribution width (RDW), mean value of respiratory rate and temperature, and the use of vasopressor. Compared with Sequential Organ Failure Assessment (SOFA) and Logistic Organ Dysfunction System (LODS), the nomogram exhibited better discrimination with an area under the receiver operating characteristic curve (AUROC) of 0.763 (95%CI 0.736–0.791, p < 0.001) and 0.753 (95%CI 0.713–0.794, p < 0.001) in the training and validation sets, respectively. The calibration plot revealed an adequate fit of the nomogram for predicting the risk of 30-day mortality in both sets. Regarding to clinical usefulness, the DCA of the nomogram exhibited greater net benefit than SOFA and LODS in both of the training and validation sets. Besides, the nomogram exhibited acceptable discrimination, calibration, and clinical usefulness in sensitivity analysis. Conclusions SAE is related to increased 30-day mortality of patients with sepsis. The nomogram presents excellent performance in predicting 30-day risk of mortality in SAE patients, which can be used to evaluate the prognosis of patients with SAE and may be more beneficial once specific treatments towards SAE are developed.
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- 2020
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14. Successful implementation of an enhanced recovery after surgery (ERAS) protocol reduces nausea and vomiting after infratentorial craniotomy for tumour resection: a randomized controlled trial
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Dan Lu, Yuan Wang, Tianzhi Zhao, Bolin Liu, Lin Ye, Lanfu Zhao, Binfang Zhao, Mingjuan Li, Lin Ma, Zhengmin Li, Jiangtao Niu, Wenhai Lv, Yufu Zhang, Tao Zheng, Yafei Xue, Lei Chen, Long Chen, Xude Sun, Guodong Gao, Bo Chen, and Shiming He
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Enhanced recovery after surgery (ERAS) ,Postoperative nausea and vomiting (PONV) ,Infratentorial craniotomy ,Anxiety ,Sleep quality ,Neurology. Diseases of the nervous system ,RC346-429 - Abstract
Abstract Background Infratentorial craniotomy patients have a high incidence of postoperative nausea and vomiting (PONV). Enhanced Recovery After Surgery (ERAS) protocols have been shown in multiple surgical disciplines to improve outcomes, including reduced PONV. However, very few studies have described the application of ERAS to infratentorial craniotomy. The aim of this study was to examine whether our ERAS protocol for infratentorial craniotomy could improve PONV. Methods We implemented an evidence-based, multimodal ERAS protocol for patients undergoing infratentorial craniotomy. A total of 105 patients who underwent infratentorial craniotomy were randomized into either the ERAS group (n = 50) or the control group (n = 55). Primary outcomes were the incidence of vomiting, nausea score, and use of rescue antiemetic during the first 72 h after surgery. Secondary outcomes included postoperative anxiety level, sleep quality, and complications. Results Over the entire 72 h post-craniotomy observation period, the cumulative incidence of vomiting was significantly lower in the ERAS group than in the control group. Meanwhile, the incidence of vomiting was significantly lower in the ERAS group on postoperative days (PODs) 2 and 3. Notably, the proportion of patients with mild nausea (VAS 0–4) was higher in the ERAS group as compared to the control group on PODs 2 or 3. Additionally, the postoperative anxiety level and quality of sleep were significantly better in the ERAS group. Conclusion Successful implementation of our ERAS protocol in infratentorial craniotomy patients could attenuate postoperative anxiety, improve sleep quality, and reduce the incidence of PONV, without increasing the rate of postoperative complications. Trial registration ChiCTR-INR-16009662 , 27 Oct 2016, Clinical study on the development and efficacy evaluation of Enhanced Recovery After Surgery (ERAS) in Neurosurgery.
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- 2020
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15. Strategy of awake surgical resection for glioma based on intraoperative functional mapping and monitoring: A case report
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Yingwu Shi, Shunnan Ge, Peigang Ji, Jinghui Liu, Yuan Wang, Shaochun Guo, Yulong Zhai, Min Chao, Guodong Gao, Yan Qu, and Liang Wang
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awake surgery ,brain mapping ,intraoperative ultrasound ,low-grade glioma ,neuronavigation ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
We reported a case of awake surgery for the left frontal low-grade glioma and reviewed the literature on the strategy of awake surgical resection for glioma. The eloquent cerebral areas that are involved in motor, language, memory, and visuospatial functions, which have to be preserved during surgery, is identified through intraoperative use of brain mapping techniques. This technique of combining intraoperative ultrasound and neuronavigation enabled extension of the surgical indications and improved the extent of resection, while minimizing postoperative morbidity and safeguarding the patient's quality of life. This work was approved by the Institutional Review Board of Tangdu Hospital, Fourth Military Medical University, China.
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- 2020
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16. Clinical Relevance of Gain- and Loss-of-Function Germline Mutations in STAT1: A Systematic Review
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Wenjing Zhang, Xuemei Chen, Guodong Gao, Shubin Xing, Lina Zhou, Xuemei Tang, Xiaodong Zhao, and Yunfei An
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signal transducer and activator of transcription 1 ,chronic mucocutaneous candidiasis ,Mendelian susceptibility to mycobacterial diseases ,GoF ,LOF ,hypothyroidism ,Immunologic diseases. Allergy ,RC581-607 - Abstract
Background: Germline mutations in signal transducer and activator of transcription 1 (STAT1), which lead to primary immunodeficiency, are classified as defects in intrinsic and innate immunity. To date, no comprehensive overview comparing GOF with LOF in early-onset immunodeficiency has been compiled.Objective: To collect and systematically review all studies reporting STAT1 GOF and LOF cases, and to describe the clinical, diagnostic, molecular, and therapeutic characteristics of all the conditions.Methods: A systematic review of the PubMed, EMBASE, Web of Science, Scopus, and Cochrane to identify articles published before May 23, 2020. Data pertaining to patients with a genetic diagnosis of STAT1 GOF or LOF germline mutations, along with detailed clinical data, were reviewed.Results: The search identified 108 publications describing 442 unique patients with STAT1 GOF mutations. The patients documented with chronic mucocutaneous candidiasis (CMC; 410/442), lower respiratory tract infections (210/442), and autoimmune thyroid disease (102/442). Th17 cytopenia was identified in 87.8% of those with GOF mutations. Twenty-five patients with GOF mutations received hematopoietic stem cell transplantation (HSCT), and 10 died several months later. Twelve of 20 patients who received JAK inhibitor therapy showed improved symptoms. Twenty-one publications described 39 unique patients with STAT1 LOF mutations. The most common manifestations were Mendelian susceptibility to mycobacterial diseases (MSMD) (29/39), followed by osteomyelitis (16/39), and lymphadenopathy (9/39). Missense, indel, and frameshift mutations were identified as LOF mutations. There were no obvious defects in lymphocyte subsets or immunoglobulin levels. Eighteen patients required antimycobacterial treatment. Three patients received HSCT, and one of the three died from fulminant EBV infection.Conclusions: STAT1 GOF syndrome is a clinical entity to consider when confronted with a patient with early-onset CMC, bacterial respiratory tract infections, or autoimmune thyroid disease as well as Th17 cytopenia and humoral immunodeficiency. HSCT is still not a reasonable therapeutic choice. Immunoglobulin replacement therapy and JAK inhibitors are an attractive alternative. STAT1 LOF deficiency is a more complicated underlying cause of early-onset MSMD, osteomyelitis, respiratory tract infections, and Herpesviridae infection. Anti-mycobacterial treatment is the main therapeutic choice. More trials are needed to assess the utility of HSCT.
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- 2021
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17. Microscopic decompressive laminectomy versus percutaneous endoscopic decompressive laminectomy in patients with lumbar spinal stenosis: protocol for a systematic review and meta-analysis
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Hua Zhang, Guodong Gao, Xiuxia Li, Xiaogang Zhang, Daping Qin, and Guodong Yang
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Medicine - Published
- 2020
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18. Propoxyphene Mediates Oxyhemoglobin-Induced Injury in Rat Cortical Neurons Through Up-Regulation of Active-β-Catenin
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Yuqian Li, Jiancai Wang, Zhihong Li, Hongyu Cheng, Zhuo Zhang, Tao Luo, Xingye Zhang, Guodong Gao, Huashan Lu, and Lihong Li
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Propoxyphene ,active-β-catenin ,apoptosis ,traumatic subarachnoid hemorrhage ,mitochondrial ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Wnt/β-catenin signaling is involved in various biological processes, including the development of the central nervous system. The dysfunction of mitochondria has been shown to participate in the progress of subarachnoid hemorrhage (SAH). Traumatic subarachnoid hemorrhage (tSAH) is a serious complication in acute craniocerebral trauma. Opioids can activate the canonical Wnt/β-catenin signaling pathway. c-Myc, a downstream protein of Wnt/β-catenin signaling, contributes to the fusion of mitochondria. Here, we investigated the protective roles of Propoxyphene (Pro) against Oxyhemoglobin (OxyHb)-induced primary cultured neuron apoptosis. The data indicated that Pro rescued active-β-catenin from OxyHb-induced decline. Furthermore, Pro attenuated OxyHb-induced apoptosis and fission of mitochondria in primary cortical neurons. However, the protective effects were abrogated under active-β-catenin-deficient conditions. Together, the data presented here showed that Pro, a weak opioid analgesic drug, attenuates OxyHb-induced mitochondria-dependent apoptosis in an active-β-catenin-c-Myc-dependent manner.
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- 2020
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19. Chinese expert consensus on programming deep brain stimulation for patients with Parkinson’s disease
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Shengdi Chen, Guodong Gao, Tao Feng, Jianguo Zhang, The Chinese Medical Association neurosurgery branch of functional neurosurgery group, The Chinese Medical Association neurology branch of Parkinson’s disease, Movement disorders group, The Chinese Physician Association neurosurgeon branch of functional neurosurgery expert committee, The Chinese Physician Association neurologist branch of Parkinson’s disease and Movement disorders professional committee, and Chinese expert committee on Deep Brain Stimulation for Parkinson’s Disease
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Neurology. Diseases of the nervous system ,RC346-429 - Abstract
Abstract Background Deep Brain Stimulation (DBS) therapy for the treatment of Parkinson’s Disease (PD) is now a well-established option for some patients. Postoperative standardized programming processes can improve the level of postoperative management and programming, relieve symptoms and improve quality of life. Main body In order to improve the quality of the programming, the experts on DBS and PD in neurology and neurosurgery in China reviewed the relevant literatures and combined their own experiences and developed this expert consensus on the programming of deep brain stimulation in patients with PD in China. Conclusion This Chinese expert consensus on postoperative programming can standardize and improve postoperative management and programming of DBS for PD.
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- 2018
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20. Retrospective single-center experience with the transversus abdominis muscle release procedure in complex abdominal wall reconstruction
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Yonggang Huang, Ping Wang, Jing Ye, Guodong Gao, Fangjie Zhang, and Hao Wu
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abdominal wall reconstruction ,incisional hernia ,posterior component separation ,transversus abdominis muscle release ,Surgery ,RD1-811 - Abstract
OBJECTIVE: The objective of the study was to investigate the clinical utility of the transversus abdominis muscle release (TAR) procedure in complex abdominal wall reconstruction. MATERIALS AND METHODS: Retrospective study of 32 patients with complex abdominal wall defects admitted to Hangzhou First People's Hospital between January 2016 and December 2017. Clinical materials were collected and analyzed. RESULTS: Among 32 cases of large incisional hernias, there were 19 males (59.4%) and 13 females (40.6%). Mean age was 64.41 ± 12.11 years, body mass index was 30.00 ± 5.97 kg/m2, and mean width of the abdominal defect was 11.34 ± 1.82 cm. Twenty-four cases were midline incisional hernias with one case of planned incisional hernia after severe pancreatitis, and three cases were lateral. Five cases were recurrent incisional hernias. All patients underwent retromuscular mesh repair, with abdominal wall reconstruction using the TAR procedure. The operative time was 151.59 ± 28.64 min, and estimated blood loss was 118.12 ± 83.41 cm3. Length of hospital stay was 13.66 ± 2.72 days. Two cases had a superficial surgical site infection, five had Type II seroma, and one had intestinal obstruction. All postoperative complications resolved with nonsurgical therapy. No mesh infection, fistula, recurrence, or postoperative bulging was reported during follow-up. CONCLUSION: Posterior component separation through TAR is a reliable and effective technique for complex abdominal wall reconstruction. Long-term follow-up is needed to assess potential recurrence.
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- 2018
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21. Glucose Deficiency Elevates Acid-Sensing Ion Channel 2a Expression and Increases Seizure Susceptibility in Temporal Lobe Epilepsy
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Haitao Zhang, Guodong Gao, Yu Zhang, Yang Sun, Huanfa Li, Shan Dong, Wei Ma, Bei Liu, Weiwen Wang, Hao Wu, and Hua Zhang
- Subjects
Medicine ,Science - Abstract
Abstract Brain hypometabolism is a common epilepsy-related finding in both patients and animal models. Fluorodeoxyglucose positron emission tomography studies have shown that recurrent seizures lead to reduced glucose metabolism in certain brain regions, but no studies have definitively determined whether this induces epileptogenesis. There is evidence that acid-sensing ion channel 2a (ASIC2a) affects epilepsy susceptibility. Transcription factor CP2 (TFCP2) regulates ASIC2a expression. We report that suppressed TFCP2 expression and elevated ASIC2a expression were associated with glucose hypometabolism in the hippocampi of humans with epilepsy and of rat epilepsy model brains. In cultured PC12 cells, we determined that glucose deficiency led to TFCP2 downregulating ASIC2a. Moreover, electrophysiological recordings from cultured rat hippocampal slices showed that ASIC2a overexpression resulted in more action potentials in CA1 pyramidal neurons and increased seizure susceptibility. Our findings suggest that hippocampal glucose hypometabolism elevates ASIC2a expression by suppressing TFCP2 expression, which further enhances the intrinsic excitability of CA1 pyramidal neurons and increases seizure susceptibility in patients with temporal lobe epilepsy.
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- 2017
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22. Retraction Note: Successful implementation of an enhanced recovery after surgery (ERAS) protocol reduces nausea and vomiting after infratentorial craniotomy for tumour resection: a randomized controlled trial
- Author
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Dan Lu, Yuan Wang, Tianzhi Zhao, Bolin Liu, Lin Ye, Lanfu Zhao, Binfang Zhao, Mingjuan Li, Lin Ma, Zhengmin Li, Jiangtao Niu, Wenhai Lv, Yufu Zhang, Tao Zheng, Yafei Xue, Lei Chen, Long Chen, Xude Sun, Guodong Gao, Bo Chen, and Shiming He
- Subjects
Neurology. Diseases of the nervous system ,RC346-429 - Abstract
This article has been retracted. Please see the Retraction Notice for more detail: https://doi.org/10.1186/s12883-020-02027-1.
- Published
- 2020
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23. GSK-3β Inhibitor Alsterpaullone Attenuates MPP+-Induced Cell Damage in a c-Myc-Dependent Manner in SH-SY5Y Cells
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Jiancai Wang, Yuqian Li, Li Gao, Fengqi Yan, Guodong Gao, and Lihong Li
- Subjects
Parkinson’s disease ,mitochondria ,wnt signaling ,c-Myc ,alsterpaullone ,Neurosciences. Biological psychiatry. Neuropsychiatry ,RC321-571 - Abstract
Mitochondrial dysfunction plays significant roles in the pathogenesis of Parkinson’s Disease (PD). The inactivation of c-Myc, a down-stream gene of Wnt/β-catenin signaling, may contribute to the mitochondria dysfunction. Inhibition of glycogen synthase kinase 3β (GSK-3β) with Alsterpaullone (Als) can activate the down-stream events of Wnt signaling. Here, we investigated the protective roles of Als against MPP+-induced cell apoptosis in SH-SY5Y cells. The data showed that Als effectively rescued c-Myc from the MPP+-induced decline via Wnt signaling. Furthermore, Als protected SH-SY5Y cells from the MPP+-induced mitochondrial fission and cell apoptosis. However, the protective roles of Als were lost under β-catenin-deficient conditions. These findings indicate that Als, a GSK-3β inhibitor, attenuated the MPP+-induced mitochondria-dependent apoptotic via up-regulation of the Wnt signaling.
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- 2018
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24. Pentazocine Protects SN4741 Cells Against MPP+-Induced Cell Damage via Up-Regulation of the Canonical Wnt/β-Catenin Signaling Pathway
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Jiancai Wang, Jintao Gu, Hao Wu, Gang Zhu, Dayun Feng, Yuqian Li, Wei Guo, Keyong Tian, Guodong Gao, and Li Gao
- Subjects
β-catenin ,Parkinson’s disease ,pentazocine ,MPP+ ,Dkk1 ,Neurosciences. Biological psychiatry. Neuropsychiatry ,RC321-571 - Abstract
The Wnt/β-catenin signaling pathway has been linked to many neurodegenerative diseases including Parkinson’s disease (PD). A glycoprotein named Dickkopf-1 (Dkk1) can combine with the receptor complex on cell membrane to inhibit Wnt/β-catenin signaling. Opioids, a series of compounds including morphine, fentanyl and pentazocine, have been reported to contribute to the up-regulation of Wnt/β-catenin signaling. Naloxone is an antagonist that has been used as an antidote to opioids through mu-opioid receptor. 1-methyl-4-phenylpyridinium (MPP+), which serves as a selective toxin for dopaminergic neurons, has been used to create experimental models of PD. In our study, we examined the protective effects of pentazocine against MPP+-induced cell death in the nigral dopaminergic cell line, SN4741 and tried to elucidate the molecular mechanisms underlying such protective effects. The data showed that pretreatment with pentazocine significantly rescued the SN4741 cell against MPP+. Moreover, the MPP+-exposed SN4741 cells exhibited a down-regulation of β-catenin, which could be restored by treatment with pentazocine. However, Dkk1 but not naloxonewas associated with the abrogation of protective effect of pentazocine. These results suggest that pentazocine alleviates MPP+-induced SN4741 cells apoptosis via the up-regulation of canonical Wnt/β-catenin signaling.
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- 2017
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25. RETRACTED ARTICLE: Single-wall carbon nanohorns inhibited activation of microglia induced by lipopolysaccharide through blocking of Sirt3
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Lihong Li, Jinqian Zhang, Yang Yang, Qiang Wang, Li Gao, Yanlong Yang, Tao Chang, Xingye Zhang, Guoan Xiang, Yongmei Cao, Zujin Shi, Ming Zhao, and Guodong Gao
- Subjects
Single-walled carbon nanohorns ,Septic encephalopathy ,Cell proliferation ,Apoptosis ,Lipopolysaccharide ,Sepsis ,Materials of engineering and construction. Mechanics of materials ,TA401-492 - Abstract
Abstract Single-wall carbon nanohorns (SWNHs) have been demonstrated to accumulate in cytotoxic levels within organs of various animal models and cell types, which emerge as a wide range of promising biomedical imaging. Septic encephalopathy (SE) is an early sign of sepsis and associated with an increased rate of morbidity and mortality. Microglia activation plays an important role in neuroinflammation, which contributes to neuronal damage. Inhibition of microglia activation may have therapeutic benefits, which can alleviate the progression of neurodegeneration. Therefore, we investigated the functional changes of mice microglia cell lines pre-treated with or without lipopolysaccharide (LPS) induced by SWNHs. To address this question, the research about direct role of SWNHs on the growth, proliferation, and apoptosis of microglia cell lines in mice (N9 and BV2) pre-treated with or without LPS had been performed. Our results indicate that the particle diameter of SWNHs in water is between 342 to 712 nm. The images in scanning electron microscope showed that SWNHs on polystyrene surface are individual particles. LPS induced activation of mice microglia, promoted its growth and proliferation, and inhibited its apoptosis. SWNHs inhibited proliferation, delayed mitotic entry, and promoted apoptosis of mice microglia cells. The effects followed gradually increasing cultured time and concentrations of SWNHs, especially in cells pre-treated with LPS. SWNHs induced a significantly increase in G1 phase and inhibition of S phase of mice microglia cells in a dose-manner dependent of SWNHs, especially in cells pre-treated with LPS. The transmission electron microscope images showed that individual spherical SWNH particles smaller than 100 nm in diameters were localized inside lysosomes of mice microglia cells. SWNHs inhibited mitotic entry, growth and proliferation of mice microglia cells, and promoted its apoptosis, especially in cells pre-treated with LPS. SWNHs inhibited expression of Sirt3 and energy metabolism related with Sirt3 in mice microglia cells in a dose-dependent manner, especially in cells pre-treated with LPS. The role of SWNHs on mice microglia was implicating Sirt3 and energy metabolism associated with it.
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- 2013
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26. Chaperone-Mediated Autophagy and Mitochondrial Homeostasis in Parkinson’s Disease
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Ruixin Yang, Guodong Gao, Zixu Mao, and Qian Yang
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Neurology. Diseases of the nervous system ,RC346-429 - Abstract
Parkinson’s disease (PD), a complex neurodegenerative disorder, is pathologically characterized by the formation of Lewy bodies and loss of dopaminergic neurons in the substantia nigra pars compacta (SNc). Mitochondrial dysfunction is considered to be one of the most important causative mechanisms. In addition, dysfunction of chaperone-mediated autophagy (CMA), one of the lysosomal proteolytic pathways, has been shown to play an important role in the pathogenesis of PD. An exciting and important development is recent finding that CMA and mitochondrial quality control may be linked. This review summarizes the studies revealing the link between autophagy and mitochondrial function. Discussions are focused on the connections between CMA and mitochondrial failure and on the role of MEF2D, a neuronal survival factor, in mediating the regulation of mitochondria in the context of CMA. These new findings highlight the need to further explore the possibility of targeting the MEF2D-mitochondria-CMA network in both understanding the PD pathogenesis and developing novel therapeutic strategies.
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- 2016
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27. Nanosized TiO2-induced reproductive system dysfunction and its mechanism in female mice.
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Xiaoyang Zhao, Yuguan Ze, Guodong Gao, Xuezi Sang, Bing Li, Suxin Gui, Lei Sheng, Qingqing Sun, Jie Cheng, Zhe Cheng, Renping Hu, Ling Wang, and Fashui Hong
- Subjects
Medicine ,Science - Abstract
Recent studies have demonstrated nanosized titanium dioxide (nano-TiO2)-induced fertility reduction and ovary injury in animals. To better understand how nano-TiO2 act in mice, female mice were exposed to 2.5, 5, and 10 mg/kg nano-TiO2 by intragastric administration for 90 consecutive days; the ovary injuries, fertility, hormone levels, and inflammation-related or follicular atresia-related cytokine expression were investigated. The results showed that nano-TiO2 was deposited in the ovary, resulting in significant reduction of body weight, relative weight of ovary and fertility, alterations of hematological and serum parameters and sex hormone levels, atretic follicle increases, inflammation, and necrosis. Furthermore, nano-TiO2 exposure resulted in marked increases of insulin-like growth factor-binding protein 2, epidermal growth factor, tumor necrosis factor-α, tissue plasminogen activator, interleukin-1β, interleukin -6, Fas, and FasL expression, and significant decreases of insulin-like growth factor-1, luteinizing hormone receptor, inhibin α, and growth differentiation factor 9 expression in mouse ovary. These findings implied that fertility reduction and ovary injury of mice following exposure to nano-TiO2 may be associated with alteration of inflammation-related or follicular atresia-related cytokine expressions, and humans should take great caution when handling nano-TiO2.
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- 2013
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28. Inhibition of STAT3 reduces astrocytoma cell invasion and constitutive activation of STAT3 predicts poor prognosis in human astrocytoma.
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Qinchuan Liang, Chenkai Ma, Yang Zhao, Guodong Gao, and Jie Ma
- Subjects
Medicine ,Science - Abstract
Astrocytoma cells characteristically possess high invasion potentials. Recent studies have revealed that knockdown of signal transducers and activators of transcription 3 (STAT3) expression by RNAi induces apoptosis in astrocytoma cell. Nevertheless, the distinct roles of STAT3 in astrocytoma's invasion and recurrence have not been elucidated. In this study, we silenced STAT3 using Small interfering RNAs in two human glioblastoma multiforme (GBM) cell lines (U251 and U87), and investigated the effect on GBM cell adhesion and invasion. Our results demonstrate that disruption of STAT3 inhibits GBM cell's adhesion and invasion. Knockdown of STAT3 significantly increased E-cadherin but decreased N-cadherin, vascular endothelial growth factor, matrix metalloproteinase 2 and matrix metalloproteinase 9. Additionally, expression of pSTAT3(Tyr705) correlates with astrocytoma WHO classification, Karnofsky performance status scale score, tumor recurrence and survival. Furthermore, pSTAT3(Tyr705) is a significant prognostic factor in astrocytoma. In conclusion, STAT3 may affect astrocytoma invasion, expression of pSTAT3(Tyr705) is a significant prognostic factor in tumor recurrence and overall survival in astrocytoma patients. Therefore, STAT3 may provide a potential target for molecular therapy in human astrocytoma, and pSTAT3(Tyr705)could be an important biomarker for astrocytoma prognosis.
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- 2013
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29. Synergetics and Acoustic Emission Approach for Crazing Nonlinear Dynamical Systems.
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Guodong Gao and Yongming Xing
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- 2020
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30. NdCo3 Molecular Catalyst Coupled with a BiVO4 Photoanode for Photoelectrochemical Water Splitting
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Guodong Gao, Rong Chen, Qingjie Wang, Daniel Wun Fung Cheung, Jia Zhao, and Jingshan Luo
- Subjects
Materials Chemistry ,Electrochemistry ,Energy Engineering and Power Technology ,Chemical Engineering (miscellaneous) ,Electrical and Electronic Engineering - Published
- 2023
31. Study on the Mechanism of Pathological Recognition Based on Bioelectrical Impedance Spectrum to the Elbow Joint
- Author
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Guodong Gao, Li Pan, Ping Zhang, Kai Su, and Jirong Zhao
- Subjects
General Immunology and Microbiology ,General Medicine ,General Biochemistry, Genetics and Molecular Biology - Abstract
The elbow joint is prone to stiffness and adhesion after trauma or surgery. High-energy trauma easily led to loss of mobility of the elbow joint. Mild trauma can also cause stiffness in the elbow joint. In order to investigate the pathogenesis of stiffness and adhesion in elbow joint, a feedback-type elbow joint control system based on network is proposed in this paper. The simulation results show that it has significant differences in elbow bioelectrical impedance in patients with elbow trauma, and this new method realizes the control strategy of converting the patient’s elbow joint pathological information into elbow joint orthosis control information.
- Published
- 2023
32. Study on Intelligent Traditional Chinese Medicine Fumigation for Treating Lumbar Intervertebral Disc Herniation Based on Medical Big Data Mining
- Author
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Jirong Zhao, Ping Zhang, and Guodong Gao
- Subjects
Lumbar Vertebrae ,Article Subject ,Fumigation ,Humans ,Data Mining ,Radiology, Nuclear Medicine and imaging ,Medicine, Chinese Traditional ,Intervertebral Disc Displacement - Abstract
With the improvement of the traditional Chinese medicine fumigation (TCMF), more and more people are studying lumbar intervertebral disc herniation (LIDH) by TCMF. In order to clarify the thermodynamic mechanism of TCMF to LIDH and provide a model reference for individualized diagnosis, the lower control system is compiled by the microprocessor, and the upper control system is compiled by computer technology of VB. In this new system, the medical information of patients is recorded in the databases by the upper control system, and clinical diagnosis and treatment experience are packaged in the lower control system. The simulation results and clinical examples show that the new control system of TCMF has better clinical efficacy for LIDH patients, which not only effectively improves the pain symptoms of LIDH patients but is also economical and safe.
- Published
- 2022
33. Automatic test system development for digital beam position monitor of HEPS and BEPCII
- Author
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Xuhui Tang, Yaoyao Du, Jianshe Cao, Shujun Wei, Zhi Liu, Qiang Ye, Huizhou Ma, Jing Yang, Guodong Gao, Yukun Li, Yanfeng Sui, and Junhui Yue
- Subjects
Nuclear and High Energy Physics ,Nuclear Energy and Engineering - Published
- 2022
34. USP30 impairs mitochondrial quality control and aggravates oxidative damage after traumatic brain injury
- Author
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Yang Wu, Qing Hu, Xun Wu, Hongbo Cheng, Jiegang Yu, Li Gao, and Guodong Gao
- Subjects
Biophysics ,Cell Biology ,Molecular Biology ,Biochemistry - Published
- 2023
35. Analysis of Bioelectrical Impedance Spectrum for Elbow Stiffness Based on Hilbert–Huang Transform
- Author
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Guodong Gao, Ping Zhang, Bin Xu, Xiaogang Zhang, QuanZeng Yang, Rong Wang, ShuHuan Han, and Zhen Quan
- Subjects
body regions ,musculoskeletal diseases ,Article Subject ,Movement ,Elbow Joint ,Elbow ,Electric Impedance ,Humans ,Computer Simulation ,Radiology, Nuclear Medicine and imaging ,musculoskeletal system - Abstract
With the advent of posttraumatic elbow rehabilitation, prevention of elbow stiffness has become a key part of the development of sports medicine. In order to clarify the time point of joint movement after internal fixation to the elbow and to provide a mechanical model for individualized diagnosis. This paper uses electromagnetic wave detection technology to quickly detect the bioelectrical impedance signal of the patient's lesion location, then passes the message to the upper control system for processing, summarizes the improved Hilbert–Huang transform to deep learning, and deep learning algorithms and computer technology are used to mine the bioelectrical impedance signal of the elbow joint. The simulation and human experiment results show that bioelectrical impedance signals can clarify the pathogenesis of elbow joint stiffness and the relationship between rehabilitation treatment time and duration. It has the advantages of low cost, high fitting accuracy, strong robustness, and noninvasiveness.
- Published
- 2022
36. Transport of the Patients Supported with Extracorporeal Life Support
- Author
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Guodong Gao
- Published
- 2023
37. Risk Mutations Identified by Whole-Exome Sequencing in 21 Patients with the 'Sandwich Fusion' Subtype of Klippel-Feil Syndrome
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Nanfang Xu, Xiaoli Gong, Kan-Lin Hung, Dongwei Fan, Yinglun Tian, Jinguo Chen, Xiangyu Hou, Guodong Gao, Shilin Xue, Ming Yan, Yuan Wei, and Shenglin Wang
- Published
- 2023
38. Building social identity-based groups to enhance online peer support for patients with chronic disease: a pilot study using mixed-methods evaluation
- Author
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Jingyuan Su, Michelle Dugas, Xitong Guo, and Guodong Gao
- Subjects
Glycated Hemoglobin ,Behavioral Neuroscience ,Social Identification ,Chronic Disease ,Humans ,Social Support ,Pilot Projects ,Peer Group ,Applied Psychology - Abstract
Online peer support is increasingly important to encourage patients with chronic diseases to engage in successful self-management. However, studies mainly focus on individual-level participation and have not fully explored how to maximize the impact of online peer support through group identification. In this study, we aim to build an online social identity-based group to examine the impact of group identity on peer support. Twenty-five participants who completed the first phase of a larger study were randomly assigned either to the treatment group (identity-based group level, n = 15, three subgroups, five members in each subgroup) or to the control group (individual-level, n = 10). All participants in both treatment and control groups received the same tasks and incentives. Peer support behavior (informational support and emotional support), task completion (knowledge learning, self-tracking behavior), and health-related outcomes (self-efficacy [SE] and HbA1c) were collected for qualitative and quantitative analysis. Results from a 3-month pilot experiment showed that the treatment group offered substantial enhancement in peer support compared to the control group. It also significantly promoted improvement in SE. However, there was no significant difference in task completion or changes in HbA1c between the two groups. The results of the content analysis suggest that having a team leader, timely responsiveness, and intergroup competition played important roles in building social identity-based online groups and subsequently generating peer support. We provide some encouraging results that indicate how online groups may be effectively designed to promote peer support.
- Published
- 2022
39. A water-stable organolead iodide material for overall photocatalytic CO2 reduction
- Author
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Rong Chen, Guodong Gao, and Jingshan Luo
- Subjects
General Materials Science ,Electrical and Electronic Engineering ,Condensed Matter Physics ,Atomic and Molecular Physics, and Optics - Published
- 2022
40. Consumer Journeys of Adolescent Girls and Young Women in South Africa: Implications for Marketing HIV Prevention Products
- Author
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Louise Kenmuir, Fulvia Veronese, Ritu Agarwal, Kenyon Crowley, Jeanna M. Piper, Lorcan McHarry, Guodong Gao, and Michelle Dugas
- Subjects
Marketing ,Gender inequality ,Economics and Econometrics ,Human immunodeficiency virus (HIV) ,medicine ,medicine.disease_cause ,Psychology ,Socioeconomics ,Consumer behaviour - Abstract
Background: Female-initiated prevention products could reduce HIV infection rates in contexts with pronounced gender inequality like South Africa, but uptake and adherence remain low when available. Insights into the behavior of target consumers are needed to effectively promote these products; however, perceptions of stigma may discourage honest reporting. Focus of the Article: To address this need, we examined differences among the consumer journeys of six segments of South African adolescent girls and young women (AGYW), who vary on sexual health beliefs, sexual experience, and self-enhancement, when buying hygiene products. Research Question: We hypothesized that segments would differ in what motivated their purchases and in engagement with different touchpoints, reflecting a need for targeted outreach strategies. Methods: 1,500 low-income, Black South African AGYW (14–25 years of age) were surveyed face-to-face in their homes about their consumer journeys when purchasing deodorant and sanitary products, with the aim of extending the insights obtained to HIV prevention. Results: We found notable similarities across segments but also several important differences underscoring the potential for tailored marketing of HIV prevention products. Among some of the segments, differences were found in prepurchase mindsets and touchpoints, retail and brand drivers, and postpurchase feelings. Recommendations for Research or Practice: These findings highlight the need for tailored outreach among AGYW and may inform the design of effective, personalized marketing strategies that enhance the appeal of HIV prevention products. Limitations: To circumvent potential stigma associated with HIV, survey questions were anchored on personal hygiene products. While this may encourage greater honesty, findings may not fully generalize to HIV prevention products.
- Published
- 2021
41. Globus pallidus neuron spike time series prediction based on local-region multi-step forecasting model.
- Author
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Yan He, Jue Wang, Qingfeng Wang, Guangjun Zhang, Julei Wang, Weixin Li, Mingming Zhang, and Guodong Gao
- Published
- 2008
- Full Text
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42. Polydatin alleviates traumatic brain injury: Role of inhibiting ferroptosis
- Author
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Dayun Feng, Kai Tao, Qing Cai, Siwei Wang, Lu Huang, Ye Xi, Huaizhou Qin, Guodong Gao, Shulei He, and Fei Li
- Subjects
Male ,0301 basic medicine ,Programmed cell death ,Cell signaling ,Cell Survival ,Traumatic brain injury ,Iron ,Biophysics ,Reversion ,SLC7A11 ,Pharmacology ,GPX4 ,Biochemistry ,Neuroprotection ,Cell Line ,Mice ,03 medical and health sciences ,0302 clinical medicine ,Glucosides ,Brain Injuries, Traumatic ,Stilbenes ,medicine ,Animals ,Ferroptosis ,Molecular Biology ,Neurons ,chemistry.chemical_classification ,biology ,business.industry ,Brain ,Cell Biology ,Phospholipid Hydroperoxide Glutathione Peroxidase ,medicine.disease ,nervous system diseases ,Mice, Inbred C57BL ,Disease Models, Animal ,Neuroprotective Agents ,030104 developmental biology ,Enzyme ,chemistry ,030220 oncology & carcinogenesis ,biology.protein ,Hemin ,business - Abstract
Secondary injury is the main cause of high mortality and poor prognosis of TBI, which has recently been suggested to be related to ferroptosis. Polydatin, a monocrystalline compound extracted from the rhizome of Polygonum, has been shown to exert potential neuroprotective effects. However, its role and mechanism in the secondary injury of TBI has not been elucidated. In this study, the inhibition of Polydatin on ferroptosis was observed both in the hemoglobin treated Neuro2A cells in vitro and in TBI mouse model in vivo, characterized by reversion of accumulation or deposition of free Fe2+, increased content of MDA, decreased activity of key REDOX enzyme GPx4, cell death and tissues loss. Although Polydatin corrected the increased mRNA levels of ferroptosis signaling molecules GPX4, SLC7A11, PTGS2, and ATP5G3 after TBI, TBI and Polydatin treatment had no significant effect on their protein expression. Notably, Polydatin could completely reverse the decrease of GPx4 activity after TBI in vivo and in vitro, and the effect was stronger than that of the classical ferroptosis inhibitor FER-1 in vitro. Further, Polydatin has been shown to reduce the severity of acute neurological impairment and significantly improve subacute motor dysfunction in TBI mice. Our findings provided translational insight into neuroprotection with Polydatin in TBI by inhibiting ferroptosis mainly depending on the maintenance of GPx4 activity.
- Published
- 2021
43. Preoperative prediction of granulation pattern subtypes in GH‐secreting pituitary adenomas
- Author
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Li Gong, Wei Guo, Guodong Gao, Shuo Zhang, Dong Jia, Xiaoyan Liu, Yan Qu, and Li-Jun Heng
- Subjects
Adenoma ,medicine.medical_specialty ,Endocrinology, Diabetes and Metabolism ,Octreotide ,030209 endocrinology & metabolism ,Growth hormone ,Gastroenterology ,03 medical and health sciences ,Cytokeratin ,0302 clinical medicine ,Endocrinology ,Pituitary adenoma ,Internal medicine ,Acromegaly ,medicine ,Humans ,Pituitary Neoplasms ,Receiver operating characteristic ,business.industry ,Area under the curve ,medicine.disease ,030220 oncology & carcinogenesis ,Growth Hormone-Secreting Pituitary Adenoma ,Neoplasm Recurrence, Local ,business ,Immunostaining ,medicine.drug - Abstract
Objective The aim of this study was to establish a preoperative prediction method for sparsely granulated (SG) growth hormone (GH) secreting pituitary adenoma, an aggressive tumor subtype with high recurrence risk, in acromegaly patients. Methods Eighty-three patients with GH-secreting pituitary adenomas were included in this study. GH measurements, cytokeratin immunostaining and electron microscopy were performed to detect granulation patterns. Preoperative factors, including general, radiological and endocrinological features and acute octreotide suppression test outcomes, were compared between SG and densely granulated (DG) groups. The predictive capabilities of these features were analyzed using a receiver operating characteristic (ROC) curve, and the most predictive features were combined to establish a grading scale. Results Thirty-nine of the 83 patients had SG GH-secreting pituitary adenomas; 44 had DG tumors. SG tumors tended to occur in younger patients and have larger diameters and volumes, higher Knosp grades, lower GH indexes and normalized insulin-like growth factor-1 (IGF-1) levels, and a lower ∆GH% after octreotide treatment. The tumor size, Knosp grade, GH index and ∆GH% after octreotide treatment had good predictive performance, with area under the curve (AUC) values ranging from 0.70 to 0.80. Combining four parameters, including diameter, Knosp grade, GH index and ∆GH% after octreotide treatment, we established a grading scale for predicting SG GH-secreting pituitary adenomas with an AUC of 0.84 and relatively high sensitivity and specificity. Conclusions We propose a predictive method for distinguishing SG and DG GH-secreting pituitary adenomas preoperatively. This method will help physicians identify candidates for presurgical medical treatment and neurosurgeons determine radical surgical strategies for high-risk tumors.
- Published
- 2021
44. Mechanism of pathological recognition based on bioelectrical impedance spectrum of elbow joint
- Author
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Ping Zhang, Kai Su, and GuoDong Gao
- Published
- 2022
45. How Digital Word-of-Mouth Affects Consumer Decision Making: Evidence from Doctor Appointment Booking
- Author
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Guodong Gao, Ritu Agarwal, and Aishwarya Deep Shukla
- Subjects
Information retrieval ,Computer science ,Feature (computer vision) ,Strategy and Management ,Word of mouth ,Consideration set ,Management Science and Operations Research ,Clickstream - Abstract
We use detailed clickstream data on online word-of-mouth (WOM) to uncover mechanisms underlying its influence on consumer decision making. A feature launch on a major doctor appointment booking platform allows us to examine the effects of online WOM on three dimensions of a consumer’s choice process: the consideration set size, the time taken to consider alternatives (web session duration), and the geographic dispersion of the choices considered. Results indicate that the effects of WOM on decision-making processes are not monotonic but rather are contingent on the abundance of WOM (number of rated doctors) in a market. When the abundance of WOM is high, the introduction of WOM makes patients consider fewer doctors, browse for a shorter duration, and focus on doctors that are geographically more proximate. In contrast, when the abundance of WOM is low, the introduction of WOM makes patients consider more doctors, browse for longer duration, and consider doctors that are geographically more dispersed. We also find that WOM can lead to a cannibalization effect: when ratings are published, the highly rated doctors reap the benefits (in the form of increased demand) at the expense of unrated doctors. Our study contributes to the extant literature on online WOM by providing new insights into how WOM influences consumer decision making and by examining this question at a more granular level than prior work. This paper was accepted by Anandhi Bharadwaj, information systems.
- Published
- 2021
46. A Mandarin edutainment system integrated virtual learning environments.
- Author
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Yue Ming 0001, Qiuqi Ruan, and Guodong Gao
- Published
- 2013
- Full Text
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47. Antagonism of Protease-Activated Receptor 4 Protects Against Traumatic Brain Injury by Suppressing Neuroinflammation via Inhibition of Tab2/NF-κB Signaling
- Author
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Jianing Luo, Wei Guo, Hao Yan, Xun Wu, Haixiao Liu, Yingwu Shi, Yan Qu, Fei Li, Guodong Gao, Dayun Feng, Wenxing Cui, Kang Guo, Hao Guo, and Kai Tao
- Subjects
0301 basic medicine ,MAPK/ERK pathway ,Physiology ,Traumatic brain injury ,Inflammation ,Pharmacology ,Mice ,03 medical and health sciences ,0302 clinical medicine ,Thrombin ,Brain Injuries, Traumatic ,medicine ,Animals ,Humans ,Receptor ,Neuroinflammation ,Adaptor Proteins, Signal Transducing ,business.industry ,General Neuroscience ,General Medicine ,medicine.disease ,030104 developmental biology ,medicine.anatomical_structure ,Original Article ,Receptors, Thrombin ,medicine.symptom ,Signal transduction ,business ,030217 neurology & neurosurgery ,Signal Transduction ,medicine.drug ,Astrocyte - Abstract
Traumatic brain injury (TBI) triggers the activation of the endogenous coagulation mechanism, and a large amount of thrombin is released to curb uncontrollable bleeding through thrombin receptors, also known as protease-activated receptors (PARs). However, thrombin is one of the most critical factors in secondary brain injury. Thus, the PARs may be effective targets against hemorrhagic brain injury. Since the PAR1 antagonist has an increased bleeding risk in clinical practice, PAR4 blockade has been suggested as a more promising treatment. Here, we explored the expression pattern of PAR4 in the brain of mice after TBI, and explored the effect and possible mechanism of BMS-986120 (BMS), a novel selective and reversible PAR4 antagonist on secondary brain injury. Treatment with BMS protected against TBI in mice. mRNA-seq analysis, Western blot, and qRT-PCR verification in vitro showed that BMS significantly inhibited thrombin-induced inflammation in astrocytes, and suggested that the Tab2/ERK/NF-κB signaling pathway plays a key role in this process. Our findings provide reliable evidence that blocking PAR4 is a safe and effective intervention for TBI, and suggest that BMS has a potential clinical application in the management of TBI. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s12264-020-00601-8) contains supplementary material, which is available to authorized users.
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- 2020
48. Comparison of Minimally Invasive Surgery Transforaminal Lumbar Interbody Fusion and TLIF for Treatment of Lumbar Spine Stenosis
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Guodong Gao, Linzhong Cao, Xiaozheng Du, Bin Xu, Ping Zhang, Xiaogang Zhang, Rong Wang, and Zhen Quan
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Medicine (General) ,Lumbar Vertebrae ,Article Subject ,Biomedical Engineering ,Blood Loss, Surgical ,Health Informatics ,Constriction, Pathologic ,Middle Aged ,R5-920 ,Spinal Fusion ,Spinal Stenosis ,Treatment Outcome ,Medical technology ,Humans ,Minimally Invasive Surgical Procedures ,Surgery ,R855-855.5 ,Biotechnology ,Research Article ,Aged - Abstract
With the development of minimally invasive technology, minimally invasive surgery transforaminal lumbar interbody fusion has become an effective way to treat lumbar spinal stenosis. Lumbar spinal stenosis is one of the common diseases that cause backache or lumbago and sciatica. This article compares and analyzes the clinical efficacy of 60 patients with lumbar spinal stenosis surgery. It can be seen that the wound by MIS-TLIF is significantly less than that of traditional open surgery, and the postoperative recovery of MIS-TLIF is faster. So, MIS-TLIF is one of the concepts of minimally invasive surgery. The age distribution ranged from 56 to 78 years, with an average of 65.7 years. 31 cases were treated with MIS-TLIF (MIS-TLIF group), and 29 were treated with traditional posterior open surgery (TLIF group). The operation time, intraoperative blood loss, and postoperative drainage of the operation area were recorded. After statistical testing, the intraoperative blood loss, incision size, and postoperative drainage volume of the wound in the MIS-TLIF group were significantly less than those in the TLIF group. The results of JOA score, ODI score, and VAS score during the postoperative follow-up period were comparable to those of open surgery. Therefore, minimally invasive transforaminal lumbar interbody fusion is effective in treating lumbar spinal stenosis.
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- 2022
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49. An investigation of pressure loss and dynamical model of reed-type valves in compressors based on Euler–Bernoulli beam theory
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Guangyu Mu, Feng Wang, and Guodong Gao
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Cantilever ,Mechanical Engineering ,ComputingMilieux_PERSONALCOMPUTING ,Data_CODINGANDINFORMATIONTHEORY ,Mechanics ,Bending ,Physics::Classical Physics ,GeneralLiterature_MISCELLANEOUS ,Vibration theory of olfaction ,Reed valve ,Limiter ,Physics::Accelerator Physics ,Euler–Bernoulli beam theory ,Hardware_ARITHMETICANDLOGICSTRUCTURES ,Gas compressor ,Beam (structure) ,Computer Science::Information Theory ,Mathematics - Abstract
Reed-type valve is widely used in refrigeration compressor; its dynamic performance directly affects the energy efficiency of compressors. In order to reveal the motion law of the reed-type valve of refrigeration compressor, a dynamic model based on vibration theory is established. According to the actual movement characteristics of the reed valve, the motion process of the reed is divided into two stages (i.e. before the reed bending to the limiter and after the reed bending to the limiter). The one-degree-of-freedom system is used to model before the valve reed bending to the limiter, and the vibration theory of Euler–Bernoulli beam is used to model after the reed bending to the limiter. The fourth-order Runge–Kutta method is applied to solve the new model in the MATLAB environment. In order to verify the validity of the new model, dynamic performance experiments of discharge reed valve at various operating conditions were carried out. The predicted results of the new model, the basic valve theory model and the cantilever beam model are compared with the experimental results. The analysis of error band and root mean square error shows that the calculation results of the new model can more accurately reveal the motion law of reed valve than that of other two models. Then, the effects of valve lift, reed stiffness and compressor speed on the valve dynamics and pressure loss are analyzed. This research can provide a reference for optimizing the structure parameters of reed-type valve and improving the energy efficiency of compressors.
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- 2020
50. Development of a nomogram to predict 30-day mortality of patients with sepsis-associated encephalopathy: a retrospective cohort study
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Rong Li, Shengru Liang, Lihong Li, Qiuhe Wang, Jie Geng, Guodong Gao, Yuan Cao, Pan Wang, and Yang Yang
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medicine.medical_specialty ,30-day mortality ,Sepsis-associated encephalopathy ,030204 cardiovascular system & hematology ,Critical Care and Intensive Care Medicine ,Nomogram ,03 medical and health sciences ,0302 clinical medicine ,Intensive care ,Internal medicine ,Sepsis ,medicine ,Risk of mortality ,RDW ,Receiver operating characteristic ,business.industry ,Research ,Organ dysfunction ,Glasgow Coma Scale ,lcsh:Medical emergencies. Critical care. Intensive care. First aid ,030208 emergency & critical care medicine ,Red blood cell distribution width ,Retrospective cohort study ,lcsh:RC86-88.9 ,medicine.symptom ,business - Abstract
Background Sepsis-associated encephalopathy (SAE) is related to increased short-term mortality in patients with sepsis. We aim to establish a user-friendly nomogram for individual prediction of 30-day risk of mortality in patients with SAE. Methods Data were retrospectively retrieved from the Medical Information Mart for Intensive Care (MIMIC III) open source clinical database. SAE was defined by Glasgow Coma Score (GCS) < 15 or delirium at the presence of sepsis. Prediction model with a nomogram was constructed in the training set by logistic regression analysis and then undergone internal validation and sensitivity analysis. Results SAE accounted for about 50% in patients with sepsis and was independently associated with the 30-day mortality of sepsis. Variables eligible for the nomogram included patient’s age and clinical parameters on the first day of ICU admission including the GCS score, lactate, bilirubin, red blood cell distribution width (RDW), mean value of respiratory rate and temperature, and the use of vasopressor. Compared with Sequential Organ Failure Assessment (SOFA) and Logistic Organ Dysfunction System (LODS), the nomogram exhibited better discrimination with an area under the receiver operating characteristic curve (AUROC) of 0.763 (95%CI 0.736–0.791, p < 0.001) and 0.753 (95%CI 0.713–0.794, p < 0.001) in the training and validation sets, respectively. The calibration plot revealed an adequate fit of the nomogram for predicting the risk of 30-day mortality in both sets. Regarding to clinical usefulness, the DCA of the nomogram exhibited greater net benefit than SOFA and LODS in both of the training and validation sets. Besides, the nomogram exhibited acceptable discrimination, calibration, and clinical usefulness in sensitivity analysis. Conclusions SAE is related to increased 30-day mortality of patients with sepsis. The nomogram presents excellent performance in predicting 30-day risk of mortality in SAE patients, which can be used to evaluate the prognosis of patients with SAE and may be more beneficial once specific treatments towards SAE are developed.
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- 2020
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