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The oncogenic role of long intergenic noncoding RNA for IGF2BP2 stability (LINRIS) has been reported in colorectal cancer. This research aimed to study its potential involvement in gastric cancer (GC). In this study, paired GC and non-tumor tissues were obtained from 64 GC patients, and the levels of LINRIS, mature microRNA-34a (miR-34a), and miR-34a precursor in these tissues were measured with RT-qPCR. Linear regression was used to analyze their correlations. The role of LINRIS overexpression and siRNA silencing in regulating the maturation of miR-34a was analyzed by RT-qPCR. Cell apoptosis was studied with flow cytometry. It was observed that LINRIS was overexpressed in GC and showed a negative correlation with mature miR-34a, but not miR-34a precursor. In GC cells, LINRIS siRNA silencing upregulated mature miR-34a level, but not miR-34a precursor level. LINRIS overexpression downregulated miR-34a level. Cell apoptosis analysis showed that LINRIS siRNA silencing and miR-34a overexpression promoted GC cell apoptosis and suppressed cell migration and invasion, while LINRIS overexpression suppressed cell apoptosis and enhanced cell migration and invasion. In addition, the effect of LINRIS overexpression was reversed by miR-34a overexpression. Therefore, LINRIS siRNA silencing in GC may promote cell apoptosis by promoting miR-34a maturation.

Objective To compare the clinical effects of coronary artery bypass grafting (CABG) between the left anterior small thoracotomy (LAST) and lower-end sternal splitting (LESS) approaches for coronary artery disease. Methods In total, 110 patients who underwent LAST from October 2015 to December 2020 in Tianjin Chest Hospital were selected as the observation group. Patients who underwent the LESS approach during the same period were analyzed. The propensity score was calculated by a logistic regression model, and nearest-neighbor matching was used for 1:1 matching. Results The length of hospital stay and ventilator support time were significantly shorter in the LAST than LESS group. The target vessels in the obtuse marginal branch and posterior left ventricular artery branch grafts were significantly more numerous in the LAST than LESS group, but those in the right coronary artery graft were significantly less numerous in the LAST group. Conclusions CABG using either the LAST or LESS approach is safe and effective, especially in low-risk patients. The LAST approach can achieve complete revascularization for multivessel lesions and has the advantages of less trauma and an aesthetic outcome. However, it requires a certain learning curve to master the surgical techniques and has specific surgical indications.

【Background】 Drought is an important factor restricting China’s agricultural development. Tianshui City of Gansu Province is located in the inland, with a warm temperate semi-humid and semi-arid climate, thin soil layer, unfertile land and lack of irrigation conditions. Natural rainfall is an important source of agricultural irrigation water, but affected by geographical location and climatic conditions, the average annual rainfall is small and uneven. As a result, crops have low utilization efficiency of rainfall. Therefore, it is particularly important to improve crop water use efficiency in arid areas. CLP type super absorbent polymer is a kind of new polymer materials, water absorption and water retention ability is very strong, containing a large number of strong water absorption, high permeability can be induced by association with and through the base mesh structure, absorb hundreds of times to thousands of times more than its weight of water, for a variety of crops, fruit trees, vegetables and so on root directly provide plenty of water, to ensure that the crop growth environment, It has positive significance to improve the water use efficiency of orchard. 【Objective】 The purpose of this paper is to study the effect of CLP type super absorbent polymer on soil physical and chemical properties and fruit quality of apple orchard in dryland. 【Method】 This experiment used crosslinked polyacrylamide (acrylamide-potassium acrylate copolymer, English name is CL-PAM-K, abbreviated as CLP) super absorbent polymer. Set four treatments, respectively were: CK: no super absorbent polymer; T1:150 g/plant; T2:250 g/plant; T3:350 g/plant. Soil water content, soil nutrients, fruit appearance quality and nutritional quality were measured to comprehensively evaluate the effects of each treatment on soil physical and chemical properties and fruit quality. 【Result】 At the bud stage, T1 treatment decreased the soil electrical conductivity by 5.70%, 10.82% and 19.64%, in the 0-20 cm, 20-40 cm and 40-60 cm soil layers, respectively. At fruit ripening stage, T1 treatment increased the soil water content by 7.26% and 39.12% in the 20-40 cm and 40-60 cm soil layers, respectively, while decreasing the pH value by 3.43%, 1.38% and 0.23% in the 0-20 cm, 20-40 cm and 40-60 cm soil layers, respectively. The fruit peel puncture strength increased by 16%. The application of 250 g/plant increased the soil water content and available nitrogen in the 0~20 cm, 20-40 cm and 40-60 cm soil layers at the bud stage, as well as the available potassium in the 0-20 cm and 40-60 cm soil layers at both the bud stage and fruit ripening stage. The application of 350 g/plant increased the available phosphorus in the 0-20 cm, 20-40 cm and 40-60 cm soil layers at both the bud stage and fruit ripening stage. The application of 250 g/plant increased the soluble protein and total phenol content of apple fruits. 【Conclusion】 The application of 350 g/plant was the optimal amount of drought-resistant soil amendment to improve soil physical and chemical properties and enhance fruit quality in dryland apple orchards.